KR100661488B1 - Tumor screening apparatus for liquid based cytology - Google Patents

Abstract

The present invention relates to a cell screening device, and more particularly, to a cell screening device performed by an automated means for separating the cells from the container containing the mixed solution of the cells to be collected on the slide. Cell test apparatus according to the present invention includes a fixed portion for fixing the solution cylinder with a piston built-in solution cylinder operating unit having a cylinder mechanism for linearly moving the solution cylinder toward the filter coupled to the solution cylinder And a cylinder rod fixed to the cylinder and a cylinder rod installed on the cylinder, wherein the cylinder rod is fixed to one side of the piston of the solution cylinder and disposed below the piston operation unit for linearly moving the piston and the solution cylinder operation unit. It is configured to include a coupling portion coupled to the coupling portion connected to the coupling portion guides the solution filled in the solution container and the suction mechanism connected to the inner space of the passage.

Uterine cells, fluids, slides, and samples

Description

Liquid cell screening device for various cancer screening {TUMOR SCREENING APPARATUS FOR LIQUID BASED CYTOLOGY}

1 is an exploded perspective view showing the configuration of a solution container according to a preferred embodiment of the present invention,

2 is a perspective view of a solution container according to a preferred embodiment of the present invention,

Figure 3 is a partially cut perspective view showing a snap coupling state of the casing and the piston of the solution container according to an embodiment of the present invention,

4 is a perspective view partially cut in the solution container according to an embodiment of the present invention,

Figure 5 is a perspective view showing the upper portion of the filter used in the cell screening apparatus according to the present invention,

6 is a view schematically showing the structure of a filter used in the cell screening apparatus according to the present invention,

7 is a perspective view showing a lower portion of the filter used in the cell screening apparatus according to the present invention,

8 is a perspective view showing a two-row cell screening apparatus according to the present invention,

Figure 9 is a schematic side cross-sectional view showing a mixed solution forming part of the cell screening apparatus according to the present invention,

10 is an enlarged perspective view of a portion of the cell detection device of FIG. 9,

11 is a partial cross-sectional view showing a coupling state of the end of the cylinder rod of the piston operation unit of the cell screen according to the present invention and the piston of the solution cylinder,

12 is a side cross-sectional view schematically showing a lid opening and closing part of the cell screen according to the present invention,

Figure 13 is a side view showing a portion of the slide operation of the cell screen according to the present invention,

14 is a cross-sectional view schematically showing an inhalation unit of the cell screen according to the present invention,

15 is a schematic front view showing a state in which the solution container is coupled to the piston engagement release portion of the cell screen according to the present invention from the front,

Figure 16 is a schematic side view showing a state in which the solution container is coupled to the piston coupling release portion of the cell screen according to the present invention,

17 is a view schematically showing a part of the conventional solution passing through the conventional cell screening device.

The present invention relates to a cell screening device, and more particularly, to a cell screening device performed by an automated means for separating cells from a container containing a mixed solution of cells and collecting them on a slide.

In particular, the solution can be used to collect cervical cells or the like into the body, and then mix the cells into the liquid to examine the cells.

17 is a view schematically showing a part of the conventional solution passing through the conventional cell screening device. As shown in FIG. 17, the conventional solution container 70 is a container structure containing a cap for collecting cells and closed with a stopper to allow cells to be mixed into a liquid by shaking a user using a hand or an apparatus. The solution container 70 is limited to use to store the liquid, and therefore, the device for transferring the cells to the slide was provided with a separate container 80 for receiving the liquid. That is, in addition to the solution container 70, a separate container 80 is required.

Moreover, since the container 80 containing the liquid of such a conventional apparatus has an open top structure, contact with air was inevitable. In addition, the cylindrical body 83 supports the filter 86 on which the collecting membrane 87 is placed, and sucks the liquid from the container 80 by the piston 90 entering and exiting from the body 83. And the container 80 is screwed.

Conventionally, there is a separate solution container for dispersing the cells attached to the brush in the liquid, shake the solution container and pour the liquid into another container while the cells are sufficiently spread in the liquid and inhale the liquid by closely attaching the suction device under the filter. Then, the filtered cells were collected by rubbing the slides.

Separating the container from the main body to bring the membrane to the slide, the mixed liquid left in the container was poured down by gravity and lost, so that the remaining liquid could not be reused.

In addition, the process of transferring cells from the solution container to the slide is time-consuming because it must be done manually by the user, and contaminants or impurities can be transferred from the user's hand to the slide, thereby reducing the accuracy of the cell test. .

In addition, even after pouring the liquid into the container and suctioning only as much as desired by the suction device, the liquid remaining in the container is already exposed to the atmosphere, so the accuracy of the inspection is not guaranteed when reused.

In addition, in the case of separating the filter under the gauze membrane container, the liquid remaining in the gauze membrane container was rushed down, and the liquid was discarded and the discarded liquid contaminated the device, which was not sanitary.

The present invention has been made to solve the above-mentioned problems of the prior art, an object of the present invention is to carry out a cell screening device is carried out by an automated means to separate the cells from the container containing the mixed solution of cells to be collected on a slide To provide.

The object of the present invention is a solution container operating unit having a cylinder mechanism for linearly moving the solution container toward the filter coupled to the solution container including a fixed portion for fixing the solution cylinder with a built-in piston; A piston operating part including a cylinder fixed to the solution container operating part and a cylinder rod installed on the cylinder, wherein the cylinder rod is fixed to one side of a piston of the solution container to linearly move the piston; And a suction part disposed below the solution container operation part and including a coupling part coupled to the filter, a suction pipe connected to the coupling part to guide the liquid filled in the solution container, and a suction mechanism connected to an internal space of the pipe. It is achieved by a cell screening device.

In order to achieve the above object, it is preferable to further include a slide operation portion consisting of a slide mounting portion on which the slide is mounted, a filter coupled to the suction portion, and a moving mechanism for moving the slide mounting portion to contact the slide.

In addition, the apparatus of the present invention preferably further includes an alcohol accommodating portion for accommodating alcohol, and the slide operation portion further includes slide discharging means for dropping the slide on the alcohol accommodating portion.

In addition, it is preferable to further include a filter operation unit consisting of a moving part for moving the filter down the solution container and a fixing part for fixing the filter.

Apparatus of the present invention preferably further comprises a mixed liquid forming portion consisting of a coupling means to which the solution container is coupled and a rotating means to rotate the coupling means.

In addition, the apparatus of the present invention further comprises a lid opening and closing portion made of a rotating mechanism installed in the moving unit and the rotating unit coupled to the moving unit and the lid installed in the solution can enter the position between the solution container operation unit and the suction unit. It is desirable to.

The piston of the solution container includes a piston rod, and the groove formed in the piston rod is snapped to a protrusion formed on two opposing flexible plates to be fixed at a specific position of the solution container, and the two flexible plates are inserted. The device includes a piston release mechanism comprising a tapered structure, a protrusion fixed to one side of the device, a cylinder installed at a predetermined interval on the top of the protrusion, and a cylinder rod installed to allow entry and exit from the cylinder.

Other objects, specific advantages and novel features of the invention will become more apparent from the following detailed description and the preferred embodiments associated with the accompanying drawings.

Hereinafter, with reference to the accompanying drawings will be described in detail the configuration of the solution container according to a preferred embodiment of the present invention.

1 is an exploded perspective view showing the coupling relationship of the configuration of the solution container according to a preferred embodiment of the present invention, Figure 2 is a perspective view of a solution container according to a preferred embodiment of the present invention, Figure 3 is an embodiment of the present invention Figure 4 is a partially cut perspective view showing a snap coupling state of the casing and the piston of the solution container according to, Figure 4 is a perspective view partially cut away the solution container according to an embodiment of the present invention. As shown in Figures 1 to 4, the solution container according to the present invention is the casing (1), the piston 20 is installed inside the casing (1), the inner space is formed to accommodate the liquid of the casing (1) It is configured to include a gauze film mounting portion 30 coupled to one end and a lid 40 coupled to the gauze film mounting portion 30. All of the above components are formed of a synthetic resin material.

The casing 1 has a liquid receiving part 105, 2 having a smooth inner surface formed therein so as to form a cylindrical inner space, and the lower part of the liquid receiving part 105, 2 extends more than the liquid receiving part 105, 2. It has an inner diameter, the end is open, and the inner surface is located inside the liquid stirring portion 4 protruding the stirring projections 5 inward, and the outer surface of the liquid stirring portion 4 is screwed with the gauze film mounting portion 30 to be described later. A screw portion for coupling is formed, and the upper portion of the liquid receiving portion 105, 2 is a closed structure except for the rectangular through hole 7, and the liquid receiving portion 105 (2) based on the through hole 7. Opposite side) is formed with a guide slit 8 which is a rectangular passage leading to the through hole 7. The guide slit 8 is a pair of elastic materials forming a pair of first surfaces 9 of elastic material forming relatively wide surfaces and a pair of surfaces perpendicular to the first surfaces 9. It consists of the second surface 12 of, the end of each of the first surface (9) is formed with a fixing projection 10 of the triangular bar structure. The outer upper portion of the casing 1 is formed with symmetrical planar portions 14, and there is a recessed portion below it, and the outer diameter of the liquid stirring portion 4, which is below the recessed portion, increases in outer diameter again. .

The piston 20 is embedded in the liquid receiving portion 105, 2 of the casing 1, and has a cylindrical contact portion 22 having the side surface in close contact with the inner surface of the liquid receiving portion 105, 2. It consists of the rod 27 which has the square cross section formed perpendicularly in one direction from (22). The side of the contact portion 22 has a structure in which the center portion is recessed, and a sealing rubber ring 23 is installed at the recessed portion, and a plurality of stirring wings 24 protruding downward are formed on the bottom surface of the contact portion 22. . The rod is installed in the casing 1 by passing through a rectangular through hole 7 above the liquid receiving portion 105 (2), the upper end 28 of the rod 27 is formed on the first surfaces (1) of the guide slit (8). 9) is tapered so as to pass between the fixing protrusions 10 while pushing outward, and directly below the tapered upper end 28 of the rod 27, the fixing protrusion 10 of the guide slit 8 and Coupling groove 29 is formed to snap. The upper end of the rod 27 is recessed inwardly by a predetermined length, and both sides of the recessed portion penetrate outwardly.

The gauze film mounting portion 30 has a structure in which a threaded portion is formed in the inner side thereof so as to be screwed with the liquid stirring portion 4 of the casing 1, and a gauze film in which small holes are formed in the center to prevent the liquid from passing rapidly. 32 is formed. A circumferential outer wall 34 and a circumferential coupling inner wall 35 formed at a predetermined distance from the outer wall 34 are formed below the gauze film mounting portion 30. The outer surface of the outer wall 34 is formed with a screw for screwing the cap 40 to be described later, the inner side of the inner wall 35 for the coupling is formed a passage connected to the gauze film (32). Protrusions 37 are formed on the outer surface of the gauze membrane mounting portion 30 so that the user can easily fasten or release the screwing of the casing 1 and the gauze membrane mounting portion 30 by hand.

Lid 40 is coupled to the gauze membrane mounting portion 30, the inner side of one surface is formed with a screw portion for coupling with the outer wall 34 of the gauze membrane mounting portion 30 and the other fastening mechanism insertion grooves 42 are Is formed.

Next, the filter 50 used with the solution container according to the present invention will be described.

Figure 5 is a perspective view showing the upper portion of the filter used in the cell screening apparatus according to the present invention, Figure 6 is a view schematically showing the structure of the filter used in the cell screening apparatus according to the present invention, Figure 7 It is a perspective view which shows the lower part of the filter used for the cell examination apparatus which concerns on this invention. As shown in FIGS. 5 to 7, the filter 50 includes a support part for supporting a permeable / permeable coin-shaped columnar member 52 that supports a collecting membrane 51 having a laminate structure of pores formed at the center of the upper surface thereof. 53 is formed, and a circumferentially upwardly engaging coupling flange 54 is inserted into the space between the outer wall 34 of the gauze membrane mounting portion 30 and the inner wall 35 for coupling, around the support portion. On both sides of the coupling flange 54, a plurality of filters 50 are stacked up and down, and at the same time the upper side of the linear protrusion having a predetermined height on the upper surface of the filter 50 so that the stacked filters 50 can slide each other. A guide member 56 is formed, and a straight groove is formed at the upper end of the upper guide member 56 in the longitudinal direction of the upper guide member 56. At the center of the lower surface of the filter 50 corresponding to the opposite surface of the portion where the coupling flange 54 is formed, a circumferential coupling groove 29 is fitted to fit the flange protruding circumferentially of the cell screening apparatus to be described later. The lower guide member 59 of the protruding structure is formed on both sides of the circumferential coupling groove 29 is fitted into the straight groove of the upper guide member 56.

Hereinafter will be described in detail with respect to the configuration of the cell screening apparatus using the solution container according to the present invention.

Figure 8 is a perspective view showing a two-row cell detection device according to the present invention, Figure 9 is an enlarged perspective view of a portion of the cell detection device of FIG. As shown in Fig. 8 and 9, the cell screening device is a mixed solution forming unit 100 for evenly mixing the cells with the solution filled in the solution container by rotating the solution container, the solution container operation unit 110 for manipulating the solution container, Piston operation unit 130 for operating the piston 20 installed in the solution container, lid opening and closing portion 140 for automatically opening and closing the lid 40 of the solution container, filter operation unit 150 for operating the filter 50, filter ( A slide operation unit 160 for operating the slide to contact the 50, a suction unit 170 is coupled to the filter 50 and sucks the mixed solution from the solution container, alcohol containing unit 200 containing the alcohol to which the slide is locked ), A piston coupling release portion 210 for releasing the piston 20 fixed to the end of the solution container is configured.

Figure 10 is a schematic side cross-sectional view showing a mixed solution forming portion of the cell screen according to the present invention. As shown in FIG. 8 and FIG. 10, the mixed liquid forming unit 100 includes a receiving unit 105 having a drawer structure having a storage space for entering and exiting the stirring chamber 102 and containing a solution container. On the bottom of the 105, a protrusion is formed so as to be coupled to the casing 1 of the solution container, and a rotating mechanism 107 connected to a motor (not shown) through the chain 109 and rotating is installed. The cells are mixed into the liquid in the solution container by the mixed liquid forming unit 100. First, the solution container is coupled to the rotary mechanism 107 while the container 105 is taken out of the stirring chamber 102, and the container ( 105 is returned to the stirring chamber 102, and then the rotating mechanism 107 rotates the solution container.

As shown in FIGS. 8 and 9, the solution container operation unit 110 supports portions having different outer diameters of the casing 1 of the solution container from both sides, respectively, and has two stages of blocks 112 arranged side by side up and down. 113 and the lowermost block 115 fixed by a spring (not shown) to the blocks 113 positioned below the two-stage blocks 112 and 113, and the two-stage blocks 112 and 113, respectively. Two stage blocks 112 and 113 are fixed to move 113 and include a moving block 118 moving along the linear guide 119, and one side of the moving block 118 is fixed in position. It is fixed to the cylinder rod 121 installed in the (120). The lowermost block 115 has a center penetrated structure and the inner diameter of the penetrated portion of the lowermost block 115 is formed to be smaller than the outer diameter of the gauze film mounting portion 30 of the solution container so that the solution container does not escape downward. Constrains the mounting portion 30 and limits the rotation of the gauze membrane mounting portion 30.

The piston manipulation unit 130 is disposed above the two blocks 112 and 113 of each stage of the solution container manipulation unit 110 and is pneumatically fixed to the blocks 112 and 113 through the coupling rod 136. A cylinder rod 134 coupled to the cylinder 132 and a pneumatic cylinder 132 to enable linear movement, and a lower end of the cylinder rod 134 is coupled to the rod 27 of the piston 20 of the solution cylinder. . As shown in FIG. 11, the lower end of the cylinder rod 134 is formed with a branched pair of hooks 135 and the pair of hooks 135 are connected to the pierced portion of the rod of the piston 20. Snap together. The cylinder rod of the piston operation unit 130 is formed of a synthetic resin material and has flexibility.

The blocks 112 and 113 of the solution container operation unit 110 and the pneumatic cylinder 132 of the piston operation unit 130 are integrally fixed to a pair of coupling rods 136 penetrating both sides thereof. The lowermost block 115 of the solution container operation unit 110 is elastically fixed to a block 113 positioned below one of the two blocks 112 and 113 through a spring (not shown).

12 is a side cross-sectional view schematically showing a lid opening and closing part of the cell screen according to the present invention. As shown in Figures 8, 9 and 12, the lid opening and closing portion 140 is installed on the upper surface of the moving part 142 and the moving part 142 which is installed at the bottom of the solution container operation unit 110 and can move forward and backward. The lid rotates 145. The lid rotating part 145 is a structure in which the top surface is recessed so that the lid 40 is settled, three coupling protrusions 146 inserted into the insertion grooves 42 of the lid 40 are formed in the center, and the lid rotating part 145 The rotating shaft 148 extending downwards) is embedded in the moving part 142 and connected to a chain 143 driven by a motor.

As shown in FIG. 9, the filter operation unit 150 has a configuration in which the tongs 152 that bite the filter 50 are moved by the pneumatic cylinder mechanism 155 and are drawn out from the filter loading unit in which the filters 50 are loaded. The forceps are bitten by the used filter 50 and are moved to the standby position of the filter 50.

Figure 13 is a side view showing a portion of the slide operation of the cell screen according to the present invention. As shown in Figs. 8, 9 and 13, the slide operation unit 160 slides while moving between the point where the filter 50 waits to drop the slide to engage with the suction unit 170 to be described later. To the filter 50 and subsequently drop the slide in contact with the filter 50 with the cell coagulating liquid, the moving part 161 and the movement connected to the cylinder rod 169 operated by the pneumatic cylinder And a rotary mount 163 hinged to the unit 161 so as to be rotatable, and a slide mounting unit 167 elastically fixed to the bottom of the rotary unit 163. The movable unit 161 moves along the linear guide. . The rotating unit 163 has a pneumatic cylinder 165, the slide mounting portion 167 is coupled to the cylinder rod coupled to the pneumatic cylinder 165. Rotating portion 163 includes a rectangular surface extending from the hinged portion, is connected to the motor can be rotated in a predetermined angle range, the slide mounting portion 167 is square through the bolt 168 through the spring It is elastically fixed to the surface of the. The lower portion of the slide mounting portion 167 is formed with a straight guide groove so that the slide can be inserted.

14 is a cross-sectional view schematically showing the suction unit of the cell screen according to the present invention. As shown in FIGS. 8, 9 and 14, the suction unit 170 is for sucking the mixed solution of the solution container via the filter 50. The suction unit 170 is coupled to the bottom of the through portion of the filter coupling portion 172, the filter coupling portion 172 of the penetrating structure that is coupled to the bottom of the portion formed with the collecting film 51 of the filter 50 And a liquid receiving tube 182 and a filter coupler 172 coupled to a lower end of the liquid receiving tube 182 and including a liquid discharge port 187 for discharging the liquid. The liquid receiving tube support 185 includes a moving block to which the moving block is coupled to the pneumatic cylinder mechanism to move along the linear guide. The filter coupling portion 172 has a fitting portion 174 which is fitted to the circumferential coupling groove 29 of the filter 50 on the upper surface thereof, and has an inner space opened downwardly therein. The liquid guide nozzle 178 is coupled to the bottom of 174 and extends down through the inner space 176. The inner space 176 of the filter coupling portion 172 is connected to the inlet 179 for sucking air in the inner space. The suction port 179 leads to a suction device (not shown) having a structure similar to that of the syringe. The liquid receiving tube support 185 has an inner space connected to the liquid receiving tube 182, and a liquid discharge port 187 that can be selectively opened and closed is formed at one side of the inner space to discharge the liquid to the outside. It is also possible to form a reservoir in the device and to discharge the liquid into the reservoir.

As shown in FIG. 9, the alcohol accommodating part 200 accommodates alcohol for coagulating the cells of the slide and is open at the top so that the dropped slide can enter the alcohol.

Figure 15 is a schematic front view showing a state in which the solution cylinder is coupled to the piston coupling release portion of the cell screen according to the present invention from the front, Figure 16 is a solution cylinder to the piston coupling release part of the cell screen according to the present invention It is a schematic side view which shows this combined state from the side. 1, 15 and 16, the piston engaging release portion 210 is configured to release the snap of the rod of the piston 20 and the guide slit 8 of the solution container, as long as formed on the surface of the table It consists of a pneumatic cylinder mechanism disposed on top of the triangular projections (212) through the pair of triangular projections (212) and the coupling rod (214). The lower portion of the triangular protrusion 212 has a width greater than the distance between the first surfaces 9 of the guide slit 8. The pneumatic cylinder mechanism consists of a pneumatic cylinder 216 fixed to the coupling rod 214 and a cylinder rod 218 for pressing the solution cylinder.

An LCD panel 222 is provided to display the driving of the pneumatic cylinders and the position of the moving members in detail.

Hereinafter, a process of mixing cells with a solution using a solution container according to the present invention and transferring the cells from the solution container to the slide by a cell screening apparatus will be described.

First, the process of injecting the harvesting cell where the cells are collected into the solution container to mix the cells with the liquid.

The user rotates the gauze membrane mounting portion 30 to which the lid 40 is coupled by hand, separates it from the casing 1, and injects a liquid into which the cells are to be dipped into the casing 1. Thereafter, the collecting port through which cells are collected is introduced into the casing 1, and the gauze membrane mounting part 30 is coupled to the casing 1 again.

Second, the process of moving the cells from the solution container to the slide by mounting the solution container containing the collecting port to the cell detection device.

Opening the receiving portion 105 of the mixed liquid forming portion 100 is mounted to the receiving portion 105, the solution container containing the collecting port. Specifically, the lower part of the casing 1 is coupled to the protrusion of the rotating plate formed in the receiving part 105, and the receiving part 105 having the solution container coupled thereto is pushed into the stirring chamber 102. The rotating plate is rotated at a set time and speed using the control means. As the solution container rotates, the stirring protrusions 5 of the liquid stirring part 4 of the casing 1 and the stirring blades 24 of the piston 20 stir the liquid, and the cells are separated from the collecting port and the Mixed into the liquid.

Next, the solution container, which has been sufficiently mixed, is inserted between the two blocks 112 and 113 in a state where the lowermost block 115 of the solution container operating unit 110 is pushed down and lowered downward by a predetermined dimension, and then the lowest level. When the force pushing the block 115 is removed, the lowermost block 115 is raised by the restoring force of the spring, and the lowermost block (in the state where the gauze film mounting portion 30 of the solution container is caught by the penetrating portion of the lowermost block 115) Since 115) pushes up the solution container, the solution container is fixed to the solution container operating unit 110. In this state, the pneumatic cylinder of the piston operating part 130 is operated to lower the piston 20 operating rod so that the branched end of the piston 20 operating rod snaps to the penetrated portion of the rod of the piston 20. do. Therefore, in the state in which the solution container is fixed to the solution container operating part 110, the coupling of the piston 20 and the piston operation part 130 of the solution container is completed.

Next, the forceps of the filter operation unit 150 bite off the filter 50 which is slid from the filter 50 loading unit, and move to the upper position of the suction unit 170 to stop.

Next, when the moving part of the lid opening and closing part 140 moves forward, the lid 40 rotating part is located under the lid 40 of the solution container. Next, when the moving block is lowered by operating the pneumatic cylinder of the solution container operating unit 110, the two-stage blocks 112 and 113 fixed integrally to the moving block descend and the solution container also descends at the same time. At this time, the insertion grooves 42 of the lid 40 of the solution container and the protrusions of the rotary part of the lid 40 are coupled, and when the lid 40 is rotated by controlling the motor, the lid 40 and the gauze membrane mounting part 30 are rotated. ), The lid 40 is released from the gauze membrane mounting portion 30 as the screw is loosened, and at the same time, the pneumatic cylinder is operated to raise the movable block of the solution container operating part 110 by the lead of the screw coupling, thereby closing the lid 40. Make it open smoothly. When the cylinder rod is raised by operating the pneumatic cylinder of the piston operating unit 130 at the moment the lid 40 is opened, the piston 20 coupled to the end of the cylinder rod is raised to lower the internal pressure of the casing 1 of the solution container. And prevents liquid from spilling down from the solution bottle as a result. The moving part of the lid opening and closing portion 140 in which the separated lid 40 is placed moves backward.

When the pneumatic cylinder of the solution container operation unit 110 is operated while the lid 40 of the solution container is open, the movable block is lowered so that between the circumferential outer wall 34 of the casing 1 and the inner wall 35 for the circumferential coupling. The coupling flange 54 of the upper surface of the filter 50 is fitted into the space of the filter 50. At the same time, when the movable block is raised by operating the pneumatic cylinder of the suction unit 170, the fitting portion of the upper surface of the filter coupling unit 172 is fitted. 174 fits into the circumferential engagement groove 29 of the bottom surface of the filter 50. Therefore, the solution passing filter 50 and the suction unit 170 is coupled to the outside air in a blocked state. At this time, the filter 50 is supported by the forceps of the filter operation unit 150, so that each of the solution container and the suction unit 170 may be coupled to the filter 50 at the same time.

Next, when the suction mechanism connected to the filter coupler 172 is operated while the liquid discharge port 187 formed in the liquid reservoir support 185 of the suction unit 170 is closed, the inside of the filter combiner 172 and the liquid reservoir tube As the pressure inside the 182 drops, the mixed liquid is sucked down through the gauze membrane 32 of the solution container, and the mixed liquid is filtered by the collecting membrane 51 of the filter 50 so that the cells are filtered. It goes down to (182). The amount of mixed liquor sucked from the solution bottle is controlled by controlling the suction device. While the mixed liquid is discharged from the solution container, pneumatic pressure does not act on the pneumatic cylinder of the piston operation unit 130, and the piston 20 freely descends.

Next, by operating the pneumatic cylinder of the solution container operation unit 110 to raise the moving block, while operating the pneumatic cylinder of the piston operation unit 130 to raise the piston 20 of the solution container, the solution container is above the filter 50. At the same time, since the pressure inside the casing 1 drops, the mixed liquid remaining in the solution container does not fall down.

Next, in the state in which the solution container is raised, the lid opening and closing part 140 moves forward to close the lid 40 of the solution container by performing a process opposite to opening the lid 40.

When the cylinder rod is raised by operating the pneumatic cylinder of the piston operating part 130 with respect to the solution container with the lid 40 closed, the guide slit 8 of the casing 1 is raised after the piston 20 caught at the end of the cylinder rod is raised. The coupling groove 29 of the piston 20 rod snaps to the fixing protrusion 10 of the rod. Since the close contact 22 of the piston 20 is in contact with the upper part of the casing 1 in the snapped state, if the cylinder rod continues to rise, the end of the cylinder rod slides inward and is separated from the piston 20 rod. Since the lid 40 is forcibly pulled the piston 20 from the closed solution container, the inside of the casing 1 is in a vacuum state.

Next, the lowermost block 115 of the solution container operation unit 110 is pushed down to take out the solution container from the down state.

Next, when the suction container of the suction unit 170 is further operated while the solution container is separated from the filter 50, the liquid remaining in the sampling membrane 51 of the filter 50 is sucked downward.

Next, by operating the pneumatic cylinder connected to the moving unit to move the slide operation unit 160 above the filter 50 and the pneumatic cylinder connected to the slide mounting unit 167 to lower the slide mounting unit 167 to the filter 50 The slide is brought into contact with the sampling membrane 51 of the filter 50. Thereafter, when the moving part is moved left and right while the slide and the filter 50 are in contact with each other, the slide repeatedly contacts the sampling membrane 51.

Moving the slide, which has transferred the cells from the collecting membrane 51 of the filter 50, to the upper part of the alcohol accommodating part 200 and rotating the rotating part by 90 degrees, the linear guide groove is vertically positioned, so that the slide slides to accommodate the alcohol accommodating part. Dropped to 200.

Take out the slide soaked in alcohol and use it to examine the cells.

When using the solution container that was stored in the casing (1) in a vacuum after use, release the snap coupling between the piston 20 and the casing (1) through the piston coupling release portion (210). Specifically, pneumatic pressure is placed so that the triangular protrusion 212 of the piston coupling release portion 210 is inserted into the space between the first surfaces 9 on which the fixing protrusion 10 of the guide slit 8 of the solution container is formed. When the cylinder is operated and the solution container is pushed downward, the first surfaces 9 of the casing 1 are opened by the triangular protrusion 212 to release the snap coupling between the piston 20 and the casing 1 and release the piston 20. ) Drops to a position corresponding to the amount of liquid left in the solution bottle. Therefore, the piston 20 returns to a state in which the piston 20 can move freely with respect to the solution container.

The solution container according to the present invention functions as a storage container that can contain a collection port of cells to make a mixed solution, and at the same time additionally directly mounted to the cell test device to achieve the function of discharging the liquid down.

In addition, since the solution container of the present invention is provided with a stirring means, when the solution container is rotated, the liquid inside is stirred, and when the solution is automatically rotated by setting the stirring time, the same level of cells and liquids are always achieved.

In the solution container of the present invention, since the piston 20 moves, the liquid is always kept in a state of being cut off from the outside while the liquid is discharged, and external air is prevented from penetrating into the inside. Therefore, the liquid left in the solution can be prevented from being deteriorated by air, and the liquid can be reused later.

In addition, when opening the lid 40, the solution can lower the pressure inside the casing 1 by slightly raising the piston 20, so that the liquid, which is the contents, is lowered through the gauze membrane 32 due to the suction effect caused by the pressure difference. Do not pour into. Therefore, it is possible to minimize the liquid is discarded and to prevent contamination of the filter 50 and the like by the spilled liquid.

Since the mixed liquid can be stored in the state where the piston 20 rod is snapped on the upper end of the guide slit 8 of the casing 1, the inside of the solution container forms a vacuum state, and thus the liquid can be prevented from being deteriorated. .

When the solution container according to the present invention is put into the mixed liquid forming unit, the cells are evenly mixed into the liquid by the stirring protrusion and the stirring wing of the solution container while the solution container rotates.

When the mixed liquid is discharged from the solution container, the piston can freely descend so that the liquid can be discharged in an airtight and closed state.

The lid opening automatically opens or closes the lid of the solution bottle, further reducing the time required for the process.

Since the liquid guide nozzle formed in the filter coupling portion of the suction unit is located below the suction port, the pressure in the inner space is reduced, so that the liquid penetrates into the suction port even if the liquid is sucked in.

The slide operation portion is prevented from contaminating the slide by the user's hand since the user does not participate in the process of transferring the cells from the filter membrane to the slide and dropping the alcohol.

The piston disengagement section automatically snaps off the piston and the solution casing so that the solution can be quickly reused.

When using the cell screen according to the present invention can be reused the remaining mixed solution can reduce the amount of waste generated without having to use a new solution each time.

All operation status is displayed on LCD panel, so it is easy to control.

Although the present invention has been described in connection with the above-mentioned preferred embodiments, it is possible to make various modifications or variations without departing from the spirit and scope of the invention. Accordingly, the appended claims will cover such modifications and variations as fall within the spirit of the invention.

The cell screening apparatus according to the present invention has the effect of separating the cells from the container containing the mixed solution in which the cells are collected and collecting them on the slide by an automated means.

Claims (7)

A solution container operating part including a fixing part for fixing a solution container in which a piston is embedded and having a cylinder mechanism for linearly moving the solution container toward a filter coupled to the solution container; A piston operating part including a cylinder fixed to the solution container operating part and a cylinder rod installed on the cylinder, wherein the cylinder rod is fixed to one side of a piston of the solution container to linearly move the piston; And A cell disposed in the lower part of the solution container operation part and including a coupling part coupled to the filter and a suction part including a pipe line connected to the coupling part to guide the liquid filled in the solution container and a suction device connected to an inner space of the pipe line; Screening device. The method of claim 1, And a slide operation unit comprising a slide mounting unit to which a slide is mounted, a filter coupled to the suction unit, and a moving mechanism to move the slide mounting unit to contact the slide. The method of claim 2, Further comprising an alcohol accommodating portion in which the alcohol is accommodated, And the slide operation part further comprises a slide dropping means for dropping the slide on the alcohol containing portion. The method of claim 1, And a filter operating unit including a moving unit for moving the filter down the solution container and a fixing unit for fixing the filter. The method of claim 1, Cell detection device further comprises a mixed solution forming unit consisting of a coupling means for coupling the solution container and a rotating means for rotating the coupling means. The method of claim 1, Cell detection device further comprises a lid opening and closing portion made of a rotating mechanism installed in the moving unit and the moving part and the lid installed in the solution container can enter into the position between the solution container operation unit and the suction unit; . The method of claim 1, The piston of the solution container includes a piston rod and the groove formed in the piston rod is snapped to the projection formed on the two opposite flexible plates fixed to a specific position of the solution container, The two flexible plates can be inserted into a tapered structure, and a piston decoupling portion comprising a protrusion fixed to one side of the apparatus, a cylinder installed at a predetermined interval on the upper portion of the protrusion, and a cylinder rod installed to be able to enter and exit from the cylinder. Cell detection device that further comprises.

Images