KR100883566B1 - The healthy and funtional foods for the cancer prevention of using new type of solanum tuberosum l cv. gogu valley - Google Patents

Abstract

A dietary supplement having the anticancer activity, and a food additive having the anticancer activity are provided to suppress the skin cancer cell and the stomach cancer cell. A dietary supplement having the anticancer activity contains an extract of Solanum tuberosum L. (Gogu valley). Preferably the dietary supplement is prepared into a form of a raw juice, a pouch, a beverage or a tea. Also the food additive having the anticancer activity contains an extract of Solanum tuberosum L.

Description

The healthy and funtional foods for the cancer prevention of using new type of Solanum tuberosum L cv. Gogu Valley}

The present invention relates to a health functional food containing a new kind of potatoes, Solanum tuberosum L. (cv. Gogu valley) extract, and has a preventive function of cancer.

Korea has shown a distinctive disease structure since 1982. In particular, they are exposed to a number of carcinogens, including severe air pollution, pesticides, smoking, chemicals, bad diets (harmful food additives, fried instant foods, and excessive fatty foods), and stress over time. Cancer caused by these various factors is the most common cause of death as a single disease and has emerged as a social problem.

Accumulation of various harmful substances in the body will eventually contaminate the blood. Cell displacement occurs due to the production of harmful oxygen (active oxygen), which occurs during digestion and metabolism of food, and the damaged cell mass is cancer. Cancer is an abnormal tissue of white seaweed that grows freely and destroys normal tissue, and is called a malignant tumor.

There are two types of tumors, benign and malignant. Benign tumors continue to grow, but they do not significantly affect life, and surgery can heal without recurrence. However, cancer, a malignant tumor, can occur in any tissue of the body, and cancer cells generally penetrate and destroy adjacent tissues, gradually invade the circulatory system, and spread to other parts of the body far from the cancerous area, eventually leading to host (e.g., To kill a person).

Cancer is divided into solid tumors and blood cancers. Cancers in blood cells such as leukemia and lymphoma are called hematologic cancers, accounting for about 5% of all cancers. The rest are solid cancers of the organs and skin. The latter are again classified according to organs. For example, it is called stomach cancer and liver cancer. It is also classified according to the tissue that occurred. The most common solid cancer is epithelial cancer that occurs in the tissues of the epithelium (the cells that cover the intestines, respiratory tract, and urinary tract). It occurs in glandular epithelium and is called adenocarcinoma. Some occur in muscles or bones, and this is called sarcoma. For example, when cancer occurs in the epithelium of the stomach, it is called stomach adenocarcinoma, and when cancer occurs in the muscles of the stomach, it is called stomach sarcoma.

When the cancer looks smooth in accordance with the form seen under a microscope, it is called a cancer with a good degree of differentiation or a low degree of differentiation (differentiation degree 1). Conversely, if it looks dangerous, it is called a differentiation or a high (differentiation 3) cancer, and the prognosis is bad. The middle type with the highest degree of differentiation is most common. Early cancers are mostly cancers with good differentiation.

Cancer develops in stages and progresses more malignant over time. Cancer can be classified according to the stage of onset, and the concept of development is stage. The stage before cancer is called precancerous lesion. Some of the precancerous lesions are identical in form to complete cancers, but remain in the epithelium and are called epithelial cancer or stage 0 cancer.

The earliest of the cancers is stage 1 cancer with a slight degree of invasion, stage 2 cancer stays in the organ where it occurred, but it clearly invades normal tissue, stage III cancer almost penetrates the surrounding organ or invades surrounding organs. Is metastasis to lymph nodes or distal organs.

There are largely local and systemic therapies for the treatment of cancer, and the choice of treatment depends on the stage of cancer, the general condition of the patient, the desire of the patient, and the judgment of the treating physician. Topical therapies are the resection or destruction of the primary cancer and are of significant value when the stage is low, especially stage 1-2 cancer. The most common treatment is the so-called radical resection, which involves the removal of cancer under the skin incision, including the cancerous organs and the surrounding lymph nodes. On the other hand, recently, so-called conservative surgery to relieve cancer by using an instrument such as an endoscope, a laser, a freezing device, and save an organ is also popular in recent years. The following systemic treatments are added when micro-metastatic cancer is found after resection of local cancer, or when the degree of differentiation is poor. Systemic treatment is to treat metastatic cancer that has spread throughout the body and is selected in stage 4 or 3 cancers. This includes chemotherapy, immunotherapy, gene therapy, and cell therapy.

The most widely attempted chemotherapy is the use of drug sensitivity between normal and cancer cells, which is relatively less toxic to normal cells and more selective for cancer cells, but also to some extent normal cells. The damage causes side effects. Since anti-cancer drugs have a characteristic of acting anywhere in fast-dividing cells, they act not only on rapidly dividing cancer cells but also bone marrow, gastrointestinal tract, and hair follicle cells, which are normal cells, are also affected by anti-cancer drugs.

Therefore, common side effects of these anticancer drugs include temporary blood cell loss, nausea, vomiting, diarrhea, loss of appetite, and hair loss. And another problem is that cancer is often resistant to anticancer chemicals. Today, the only cancer that can be cured by anticancer drugs is blood cancer, some childhood cancers, testicular cancer, and most adult solid cancers, such as lung cancer, stomach cancer, and liver cancer, have about one-third less cancer volume and longer survival. The development of medicines and health functional foods that can suppress production has been required.

Potatoes are native to the Andes of South America and are useful crops for coarse grain crops. Potatoes are one of the four largest food crops next to rice, wheat and corn, which are grown in more than 130 countries because of their short growing period, relatively high yields per unit area, and relatively high environmental adaptability. Potatoes are not only important as staple foods in some countries, but they are also low in calories compared to other crops such as rice and barley, and are used to make a variety of processed products because nutrients such as vitamins C, B ₁ , B ₂ , and niacin are almost close to vegetables. For this reason, there are many studies on the general nutritional analysis of potatoes and physiological activities such as antioxidant activity, and the consumption of potatoes is increasing further as the food culture is westernized. This increase in consumption necessitates the development of healthier potato varieties.

Recent changes in the international agricultural environment are putting increasing pressure on food wars and seed wars, and countries are focusing their efforts on protecting crop rights in relation to food crops because food will become the weapon of the future. Korea also joined UPOV, a new plant protection international federation in 2002, and the protection period for varieties varies slightly from country to country, but at least 18 to 35 years have been enacted.

In this situation, the inventors developed various functional potato varieties and registered as national varieties (National Seed Administration, 15 new varieties and 4 varieties of application), and foreign potato varieties (Sumi and American varieties Superior, Motherland and Japanese varieties 大地, Atlantic / USA) In addition to replacing the cultivar Atlantic, they are also undergoing trial cultivation in the United States, Canada, France, Russia, Mongolia, China, Kazakhstan and India.

Potatoes are said to be useful in many diseases in the private sector. The present inventors have developed new varieties of potatoes by improving potatoes, and the new varieties of potatoes developed by the present inventors have different efficacy for each new varieties. That is, it is known that the new varieties of Valley Potato (Bora Valley, Juice Valley, Dasom Valley, Gogu Valley) are effective in enhancing human intestinal function among the new potato varieties developed by the present inventors. Korean Patent Publication No. 654231 is known that the peptide isolated from the potato ( Solanum tuberosum L cv. Gogu Valley) excellent antibacterial activity, the Republic of Korea Patent Publication No. 654232, the potato of the Golden Valley variety ( It is known that peptides isolated from Solanum tuberosum L cv. In addition, Korean Patent Publication No. 583192 discloses an antioxidant of Borlan valley varieties Potato ( Solanum tuberosum L., cv Bora valley) extract, Republic of Korea Patent No. 709238, Purple Potato ( Solanum tuberosum L., cv Bora) It is known that the ethanol extract of the valley has an anti-obesity effect by blocking mast cells from differentiating and reducing leptin protein that differentiates into mast cells.

On the other hand, Korean Patent Laid-Open Publication No. 2001-29185 discloses that potato has antibacterial and anticancer effects by inhibiting hypertension, heart disease, constipation, hepatitis and other microorganisms, but its efficacy is unknown at all.

Solanum tuberosum L., cv Gogu valley, developed by the present inventors, was filed with the National Protection Agency for seed protection (application number: 2002-13) and registered as a national variety in 2004 (registration number: 1097) As a new breed of red skinned potatoes, it was developed to be easier to eat raw than ordinary potatoes, and has excellent shelf life and does not rot even after long periods of time.

The inventors of the new varieties potato potato potato ( Solanum tuberosum L., cv Gogu valley), while in the Republic of Korea Patent Publication No. 2001-29185 describes the potato has anti-cancer activity, while ordinary potatoes show little significant anti-cancer activity, Potato extract confirmed that the action of inhibiting the proliferation of cancer cells is very large can be used as a health functional food for cancer patients and completed the present invention.

An object of the present invention to provide a health functional food that can prevent cancer by using a new varieties of Gogu Valley potatoes ( Solanum tuberosum L., cv Gogu valley).

In order to achieve the above object, the present invention contains Solanum tuberosum L. cv. Gogu valley extract, prevents cancer and provides a health functional food for the prognosis to cancer patients.

In the present invention, Solanum tuberosum L. cv. buffer) is added to the protein extract of the sweet potato valley potato extracted. The sweet potato valley potato extract may be prepared in powder form by lyophilization.

Sweet potato valley potato extract according to the present invention has an anticancer effect by inhibiting cancer cell proliferation. In another aspect, the present invention includes inhibiting the proliferation of cancer cells by the extract of a new varieties Golan Valley potato ( Solanum tuberosum L., cv. Gogu valley) registered as a national variety, not a genetically modified crop (GMO).

Golan valley potato extract of the present invention ( Solanum tuberosum L., cv. Gogu valley) extract can be developed as a health functional food as a result of toxicity test, it is also suitable for use in addition to food.

Therefore, the present invention can be prepared by the conventional method known by adding a potato valley potato extract in the usual palatability food, that is, noodles, tofu, cereals, breads, chewing gum, candy, confectionary, such as ramen, raw noodles Tablets, granules, It may be formulated into a general formulation such as pills, hard capsules, soft capsules, or liquid formulations, and may be prepared in juice, pouches, beverages, or polysaccharides. You can mix.

Health functional foods for cancer patients of the present invention can be prepared as a main ingredient or a supplementary ingredient of Solanum tuberosum L., cv. At this time, food supplements that can be used food acceptable.

The present invention has an effective effect of Solanum tuberosum L., cv Gogu valley extracts in inhibiting human skin cancer and gastric cancer cells, and since there is no cytotoxic effect on normal cells, It can be used as a dietary supplement for the prognosis of cancer patients.

Hereinafter, the present invention will be described in more detail with reference to the following examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

Example 1 Preparation of Potato Valley Potato Ethanol Extract

33.2 Kg of sweet potato valley potatoes were extracted three times with 63 L of ethanol, concentrated under reduced pressure in a rotary vacuum still (Buchi 461), and lyophilized to obtain 388 g of powder.

<Example 2> Preparation of Sweet Potato Potato Protein Extract

By applying a known method (Ana Segura et al, Mol Plant Micro Interact ., 12 (1): 16-23, 1999), 20 kg of tubers of sweet potato valley potatoes were powdered using a mortar and pestle in a state where liquid nitrogen was added. After pulverizing with 10 mM Tris-hydrochloric acid, 1.5 M lithium chloride (10 mM Tris-HCl, 1.5 M LiCl pH 7.2) mixed solvent, the protein of the potato valley potato was extracted, and dried under reduced pressure to obtain a dry powder.

Comparative Example 1 Preparation of Common Potato Ethanol Extract

In the same manner as in Example 1, the mixture was extracted from a potato, dried under reduced pressure and dried to obtain a dry powder.

Experimental Example 1 Measurement of Hamam Activity of Potato Valley Potato Extract

end. Experimental Materials and Experimental Cell Lines

As an experimental material, the extracts of the potatoes prepared in Examples 1 and 2 and Comparative Example 1 were used, and the experimental cell line was grown from a bank of Korean cell lines and sufficiently grown under constant conditions (temperature: 37 ° C., carbon dioxide: 5%). G-361 (G-361), a human skin cancer cell, and AGS, a human gastric cancer cell, were prepared.

I. Experiment method

Dilute the cell line to 2 X 10 ³ for each well of the 96-well plate, dispense 100 microliters of Media-RPM-1641 (RPMI-1640) containing cancer cells onto the plate, and attach the cell line in a 37 ° C incubator. Incubate until After the cell lines were attached, the potato extracts prepared in Examples 1, 2 and Comparative Example 1 were put in 100 micrograms per well, and cultured in a 37 ° C. incubator for 24 hours. And 50 micrograms of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide (3- (4,5-dimethyl-2-thiazolyl) in each well. ), 2,5-diphenyl-2H-tetrazolium bromide) solution was added and incubated for 4 hours at 37 ℃ incubator. The absorbance of each well was measured at 570 nm (nm) using an ELISA reader and shown in Tables 1 and 2.

All. Experiment result

Human skin cancer cell line G-361 (G-361) and human gastric cancer cell AGS (AGS) two cell lines were confirmed to be inhibited by the extract of potato yam produced in Examples 1 and 2 according to the present invention.

As can be seen in Table 1 and Table 2, Examples 1 and 2 according to the present invention shows an inhibitory effect on human skin cancer and gastric cancer cells, but it can be seen that it has anti-cancer activity, Comparative Example 1 is a regular potato Unlike the anti-cancer effects that have been orally treated by folk remedies, it can be seen that they have little effect.

Therefore, the new varieties, Golan valley ( Solanum tuberosum L., cv. Gogu valley), can be seen that the anti-cancer activity is very effective unlike the other potatoes.

Experimental Example 2 Cytotoxicity Measurement

Under normal conditions (temperature: 37 ° C, carbon dioxide: 5%), the normal keratinocyte cell line (HaCaT) of humans grown enough to dilute to 2 x 10 ³ in 96-well plate to median DM containing normal cells (DEME ) 100 microliters were aliquoted onto the plate and incubated in a 37 ° C. incubator until the cell lines adhered. After the cell line was attached, 100 micrograms of protein extract of the potato prepared in Example 2 was placed in the well, and cultured in a 37 ° C. incubator for 24 hours. And 50 micrograms of empty (MTT, 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide) solution was added to the wells and incubated for 4 hours at 37 ℃ incubator. The absorbance of the wells was measured in the 570 nm (nm) using an ELISA reader, and the results are shown in Table 3.

As can be seen in Table 3, the sweet potato valley according to the present invention potato The extract does not appear to be toxic to normal cells of humans, so it is not considered to adversely affect the human body.

1 is a potato potato potato Solanum according to the present invention tuberosum L., cv. A real photograph of the Gogu valley is shown.

Claims (6)

A dietary supplement containing the extract of Solanum tuberosum L., cv. According to claim 1, the health functional food is a tablet, granules, A health functional food having a preventive function of cancer, characterized in that selected from pills, hard capsules, soft capsules or liquid formulations. According to claim 1, wherein the health functional food is a juice, pouch, Health functional food with a preventive function of cancer, characterized in that the drink or tea. Golan valley potato ( Solanum tuberosum L., cv. Gogu valley) is a food additive with a preventive function of cancer. Health with a preventive function of cancer, characterized by containing a food additive, the extract of Solanum tuberosum L. (cv. Gogu valley) according to claim 4, in a favorite food selected from chewing gum, candy, and confectionary. Nutraceutical. Solanum potato according to claim 4 for foods selected from noodles, tofu, cereals, and breads such as ramen and raw noodles tuberosum L., cv. Gogu valley) health functional food having a preventive function of cancer, characterized in that it contains a food additive which is an extract.

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