KR100963094B1 - Pharmaceutical composition for the anticancer comprising the extracted protein or peptide from Solanum tuberosum L cv. Gogu Valley - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for anticancer containing a protein extract extracted from potato yam valley potato or a peptide isolated therefrom as an active ingredient.

Protein extract or peptide of potato yam potato according to the invention is excellent in the inhibitory activity of human skin cancer and gastric cancer cells, and also very safe in terms of toxicity can be usefully used as a pharmaceutical composition for anticancer.

Sweet potato valley, potato, peptide, protein, anticancer activity

Description

Pharmaceutical composition for the anticancer comprising the extracted protein or peptide from Solanum tuberosum L cv. Gogu Valley}

The present invention relates to a pharmaceutical composition for anticancer containing a protein extract extracted from a new kind of potato ( Solanum tuberosum L., cv. Gogu valley) or a peptide isolated therefrom as an active ingredient.

Korea has shown a distinctive disease structure since 1982. In particular, they are exposed to a number of carcinogens, including severe air pollution, pesticides, smoking, chemicals, bad diets (harmful food additives, fried instant foods, and excessive fatty foods), and stress over time. Cancer caused by these various factors is the most common cause of death as a single disease and has emerged as a social problem.

Accumulation of various harmful substances in the body will eventually contaminate the blood. Cells are displaced due to the production of harmful oxygen (active oxygen), which occurs during food digestion and metabolism, and the resulting mass of damaged cells is cancer. Cancer is an abnormal tissue of white seaweed that grows freely and destroys normal tissue, and is called a malignant tumor.

There are two types of tumors, benign and malignant. Benign tumors continue to grow, but they do not significantly affect life, and surgery can heal without recurrence. However, cancer, a malignant tumor, can occur in any tissue of the body, and cancer cells generally penetrate and destroy adjacent tissues, gradually invade the circulatory system, and spread to other parts of the body far from the cancerous area, eventually leading to host (e.g., To kill a person).

Cancer is divided into solid tumors and blood cancers. Cancers in blood cells such as leukemia and lymphoma are called hematologic cancers, accounting for about 5% of all cancers. The rest are solid cancers of the organs and skin. The latter are again classified according to organs. For example, it is called stomach cancer and liver cancer. It is also classified according to the tissue that occurred. The most common solid cancer is epithelial cancer that occurs in the tissues of the epithelium (the cells that cover the intestines, airways, and urinary tract). It occurs in glandular epithelium and is called adenocarcinoma. Some occur in muscles or bones, and this is called sarcoma. For example, when cancer occurs in the epithelium of the stomach, it is called stomach adenocarcinoma, and when cancer occurs in the muscles of the stomach, it is called stomach sarcoma.

When the cancer looks smooth in accordance with the form seen under a microscope, it is called cancer with good degree of differentiation or low degree of differentiation (differentiation degree 1), and has a good prognosis. Conversely, if it looks dangerous, it is called a differentiation or a high (differentiation 3) cancer, and the prognosis is bad. The middle type with the highest degree of differentiation is most common. Early cancers are mostly cancers with good differentiation.

Cancer develops in stages and progresses more malignant over time. Cancer can be classified according to the stage of onset, and the concept of development is stage. The stage before cancer is called precancerous lesion. Some of the precancerous lesions are identical in form to complete cancers, but remain in the epithelium and are called epithelial cancer or stage 0 cancer.

The earliest of the cancers is stage 1 cancer with a slight degree of invasion, stage 2 cancer stays in the organ where it occurred, but it clearly invades normal tissue, stage III cancer almost penetrates the surrounding organ or invades surrounding organs. Is metastasis to lymph nodes or distal organs.

There are largely local and systemic therapies for the treatment of cancer, and the choice of treatment depends on the stage of cancer, the general condition of the patient, the desire of the patient, and the judgment of the treating physician. Topical therapies are the resection or destruction of the primary cancer and are of significant value when the stage is low, especially stage 1-2 cancer. The most common treatment is the so-called radical resection, which involves the removal of cancer under the skin incision, including the cancerous organs and the surrounding lymph nodes. On the other hand, recently, so-called conservative surgery to relieve cancer by using instruments such as endoscopy, laser, and freezing equipment and to save organs has also become popular. The following systemic treatments are added when micro-metastatic cancer is found after resection of local cancer, or when the degree of differentiation is poor. Systemic treatment is to treat metastatic cancer that has spread throughout the body and is selected in stage 4 or 3 cancers. This includes chemotherapy, immunotherapy, gene therapy, and cell therapy.

The most widely attempted chemotherapy is the use of drug sensitivity between normal and cancer cells, which is relatively less toxic to normal cells and more selective for cancer cells, but also to some extent normal cells. The damage causes side effects. Since anti-cancer drugs have a characteristic of acting anywhere in fast-dividing cells, they act not only on rapidly dividing cancer cells but also bone marrow, gastrointestinal tract, and hair follicle cells, which are normal cells, are also affected by anti-cancer drugs.

Therefore, common side effects of these anticancer drugs include temporary blood cell loss, nausea, vomiting, diarrhea, loss of appetite, and hair loss. And another problem is that cancer is often resistant to anticancer chemicals. Today, the only cancer that can be cured by anticancer drugs is blood cancer, some childhood cancers, testicular cancer, and most adult solid cancers, such as lung cancer, stomach cancer, and liver cancer, have about one-third less cancer volume and longer survival. The development of medicines and health functional foods that can suppress production has been required.

Potatoes are native to the Andes of South America and are useful crops for coarse grain crops. Potatoes are one of the four largest food crops next to rice, wheat and corn, which are grown in more than 130 countries because of their short growing period, relatively high yields per unit area, and relatively high environmental adaptability. Potatoes are not only important as staple foods in some countries, but they are also low in calories compared to other crops such as rice and barley, and are used to make a variety of processed products because nutrients such as vitamins C, B ₁ , B ₂ , and niacin are almost like vegetables. For this reason, there are many studies on the general nutritional analysis of potatoes and physiological activities such as antioxidant activity, and the consumption of potatoes is increasing further as the food culture is westernized. This increase in consumption necessitates the development of healthier potato varieties.

Recent changes in the international agricultural environment are putting increasing pressure on food wars and seed wars, and countries are focusing their efforts on protecting crop rights in relation to food crops because food will become the weapon of the future. Korea also joined UPOV, a new plant protection international federation in 2002, and the protection period for varieties varies slightly from country to country, but at least 18 to 35 years have been enacted.

In this situation, the inventors developed various functional potato varieties and registered as national varieties (National Seed Service, 15 new varieties and 4 varieties of application), and foreign potato varieties (Sumi and American varieties Superior, Motherland and Japanese varieties 大地, Atlantic / USA In addition to replacing the cultivar Atlantic, they are also undergoing trial cultivation in the United States, Canada, France, Russia, Mongolia, China, Kazakhstan and India.

Potatoes are said to be useful in many diseases in the private sector. The present inventors have developed new varieties of potatoes by improving potatoes, and the new varieties of potatoes developed by the present inventors have different efficacy for each new varieties. That is, it is known that the new varieties of Valley Potato (Bora Valley, Juice Valley, Dasom Valley, Gogu Valley) are effective in enhancing human intestinal function among the new potato varieties developed by the present inventors. Korean Patent Publication No. 654231 is known that the peptide isolated from the potato ( Solanum tuberosum L cv. Gogu Valley) excellent antibacterial activity, the Republic of Korea Patent Publication No. 654232, the potato of the Golden Valley variety ( It is known that peptides isolated from Solanum tuberosum L cv. In addition, Korean Patent Publication No. 583192 discloses an antioxidant of Borlan valley varieties Potato ( Solanum tuberosum L., cv Bora valley) extract, Republic of Korea Patent No. 709238, Purple Potato ( Solanum tuberosum L., cv Bora) It is known that the ethanol extract of the valley has an anti-obesity effect by blocking mast cells from differentiating and reducing leptin protein that differentiates into mast cells.

On the other hand, Korean Patent Laid-Open Publication No. 2001-29185 discloses that potato has antibacterial and anticancer effects by inhibiting hypertension, heart disease, constipation, hepatitis and other microorganisms, but its efficacy is not known at all.

Solanum tuberosum L., cv Gogu valley, developed by the present inventors, was filed with the National Protection Agency for seed protection (application number: 2002-13) and registered as a national variety in 2004 (registration number: 1097) It is a new kind of potato with red skin, and it is more convenient to eat raw than ordinary potato, and it has excellent shelf life and does not decay even if left for a long time.

The inventors of the new varieties potato potato potato ( Solanum tuberosum L., cv Gogu valley), while in the Republic of Korea Patent Publication No. 2001-29185 describes the potato has anti-cancer activity, while ordinary potatoes show little significant anti-cancer activity, Protein extract extracted from potatoes or peptides isolated therefrom has been confirmed that the effect of inhibiting the proliferation of cancer cells is very large can be usefully used as a pharmaceutical composition for anticancer and completed the present invention.

An object of the present invention is to provide a novel medicinal use of protein extract or peptide isolated from Solanum tuberosum L. (cv Gogu valley).

In order to achieve the above object, the present invention provides an anticancer pharmaceutical composition containing a protein extract extracted from Solanum tuberosum L. cv. Gogu valley or a peptide isolated therefrom as an active ingredient.

In the present invention, Solanum tuberosum L. cv. Gogu valley extract having a cancer cell inhibitory activity is meant to contain the protein extract of the Gogur Valley potato extracted by adding a protein extraction buffer (buffer) to the Gogur Valley potato . The sweet potato valley potato extract is prepared by extracting the protein in a potato extract buffer (buffer) as a freeze-dried may be prepared in powder form.

In addition, the present invention is a step of grinding the tubers of Golan valley potato ( Solanum tuberosum L. cv. , Extracting the protein of the potato valley potato with a buffer solution of 1.5 M LiCl pH7.2), centrifuging the extract to recover the supernatant fraction of the extract, separating the fraction by chromatography and dialysis And it can be prepared by the production method consisting of a purification step.

Specifically, proteins were extracted by adding a protein extraction buffer to Solanum tuberosum L. cv. Gogu valley, followed by ion-exchange chromatography, a molecular weight of 10,000 or less, and a sample having a molecular weight of 10,000 or less (FIG. 2). Lane 3), which was subjected to reversed phase ultrafast liquid chromatography to obtain acetonitrile concentration of 20-25% (FIG. 3), and then subjected to reversed phase ultrafast liquid chromatography to SEQ ID NO. Peak 5, which is the indicated peptide (hereafter referred to as `` piti-five (PT-5) '') and peak 6, which is the peptide represented by SEQ ID NO: 2 (herein, `` piti-six (PT) -6) ').

Therefore, in the present invention, the peptide isolated from Solanum tuberosum L. cv. Gogu valley means a peptide having a molecular weight of 10,000 or less or a peptide represented by SEQ ID NO: 1 isolated therefrom and a peptide represented by SEQ ID NO: 2 .

Peptides represented by SEQ ID NO: 1 is the result of searching for similarity of the peptide sequence solanium lycopassicom (tomato-fog stress aging protein of tomato), solanium tubulosome (fruit-mature protein) and solanium chismania (water Deficient stress protein), and the peptide represented by SEQ ID NO: 2 is the c-terminal of a kunitz-type protease inhibitor or a serine protease inhibitor. ) And homology.

Solanum tuberosum L. cv. Gogu valley protein extracts and peptides of the present invention have high anticancer activity against human skin cancer cell line G-361 (G-361) and human gastric cancer cell AGS (AGS). Indicates.

The present invention can be administered in a variety of oral and parenteral formulations during actual clinical administration, with the most preferred route of administration being oral. In addition, when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants that are generally used are prepared.

Solid preparations for oral administration may include tablets, pills, powders, granules, capsules, and the like, which may include one or more excipients, for example microcrystalline cellulose, low-substituted hydroxypropyl cellulose, colloidal oxidation Silicon, calcium silicate, starch, calcium carbonate, sucrose or lactose, gelatin and the like can be mixed, and in addition to simple excipients, lubricants such as magnesium styrate talc can also be used. In addition, liquid preparations for oral administration include suspensions, solvents, emulsions, and syrups, and various excipients, for example, wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents, water and liquid paraffin. Etc. may be included. Formulations for parenteral administration include ointments, external preparations, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

In addition, the dosage and dosage of the protein extract or peptide of Solanum tuberosum L. cv. Gogu valley according to the present invention is the weight, age, sex, health status, diet, administration time, administration method, excretion of the patient The range varies depending on the rate and the severity of the disease, and protein extracts can be administered in the range of 1 mg to 500 mg / kg for adults. Peptides are divided into 0.001 to 100 mg / kg once daily or several times. desirable.

Peptides of Solanum tuberosum L. cv. Gogu valley according to the present invention was found to be nontoxic as a result of cytotoxicity test. Through these safety experiments, it is determined that the protein extract or peptide isolated from the potato of potato according to the present invention will not adversely affect the human body.

Protein extract of Solanum tuberosum L., cv Gogu valley according to the present invention or peptide isolated therefrom is excellent in the inhibitory activity of human skin cancer and gastric cancer cells, and also very safe in terms of toxicity and anticancer pharmaceutical composition It can be usefully used.

Hereinafter, the present invention will be described in more detail with reference to the following examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

<Example 1> Sweet potato valley potato protein extract according to the present invention

By applying a known method (Ana Segura et al, Mol Plant Micro Interact ., 12 (1): 16-23, 1999), 20 kg of tuber of Solanum tuberosum L., cv. Was added to the protein extraction buffer of 10 mM Tris-hydrochloric acid, 1.5 M lithium chloride (10 mM Tris-HCl, 1.5 M LiCl pH 7.2) using a mortar and pestle. Protein was extracted. The extract was centrifuged in a centrifuge (12,000 rpm) for 30 minutes, and then the supernatant was collected using a dialysis membrane (molecular weight 1,000) using 25 mM tris-hydrochloric acid, 10 mM sodium chloride, 1 mM idieti (25 mM Tris-HCl, 10). Dialysis was performed at 4 ° C. with a buffer of mM NaCl, 1 mM EDTA pH 7.50) to extract and extract the protein of the yam valley potato.

<Example 2> Peptide Separation from Sweet Potato Valley Potato Proteins According to the Present Invention

1. Protein Extraction of Sweet Potato Valley Potatoes

Protein was extracted from the potato valley potato by the same method as in Example 1.

2. Peptide separation up to 10,000 molecular weight

A) ion-exchange chromatography (ion-exchange chromatograhy)

Ion exchange chromatography using total protein equilibrated by dialysis with the 25 mM Tris-HCl, 10 mM Sodium Chloride, 1 mM IDT (25 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA pH 7.50) buffer ion-exchange chromatography) was performed. After collecting only the portion of the protein that does not bind to the resin using a DEAE resin, and using a dialysis membrane (molecular weight 1,000) with 20mM ammonium acetate pH 6.0 buffer solution at 4 ℃ secondary Dialysis was performed.

B) cutting to below 10,000 molecular weight

The total protein extracted by ion exchange resin chromatography and dialysis was cut into peptides having a molecular weight of 10,000 or more and peptides having a molecular weight of 10,000 or less using centricon having a size of molecular weight 10,000.

C) Confirmation of protein separation by electrophoresis

Tricin SDS-PAGE was performed to confirm the isolation of the protein. Samples and standard proteins were injected into 16.5% acrylamide gels. After electrophoresis, the gel was stained with Comageie brilliant blue G-250 and decolorized with 10% acetic acid to confirm the band of the protein. Molecular weight standard proteins include ultra-low molecular weight range of tritanose phosphate isomerase (26.6kDa, Triphosphphosphate isomerase), myoglobin (17kDa, Myoglobin), alpha lactalbumin (14.2 kDa, α-Lactalbumin) aprotinin 6.5kDa Aprotinin, insulin chain ratio (3.5kDa, Insulin chain B), Bradykinin (1kDa, Bradykinin) using the molecular weight of the protein was confirmed and shown in FIG.

As can be seen in Figure 2, after extraction of protein with potato potato potato, it was confirmed that after the ion-exchange chromatography, the molecular weight was cut to 10,000 or less, the peptides with small molecular weight were concentrated.

D) Reversed Phase Chromatography (RP-HPLC) Purification

Peptides separated to a molecular weight of 10,000 or less were purified by reverse phase chromatography using a reverse phase column (Vydac, 4.6 × 250 mm) of 18 carbons, subjected to reverse phase high speed chromatography (FIG. 2), and the fractions obtained therefrom. Electrophoresis (Fig. 3).

Purified peptide was increased in concentration of acetonitrile buffer containing 0.1% Trifluoroacetic acid (TFA) (10-15%, 15-20%, 20-25%, 25-30%, 30-60%) to elute the peptides in each compartment to increase the concentration of acetonitrile buffer (10-15%, 15-20%, 20-25%, 25-30%, 30-60%) Star peptides were obtained.

<Example 3> Peptide isolation and amino acid sequencing with high anticancer activity

1) Isolation of Peptides

In Example 2, the acetonitrile concentration fraction of 20-25% having the highest anticancer activity was once again subjected to reverse phase ultra high performance liquid chromatography to separate peptides of each peak from 1 to 11. .

FIG. 5 is a profile of peaks obtained by injecting a sample with a program of acetonitrile gradient of 20 to 30%, and FIG. 6 is an electrophoretic photograph.

2) Determination of amino acid sequence

Edman degration was performed to analyze the amino acid sequences of fractions 5 and 6, named PTI-FIVE (PT-5) and PTI-SIX (PT-6). Peptide fractions represented by SEQ ID NO: 1 having a molecular weight of about 7 kDa in 5) and peptide fractions represented by SEQ ID NO: 2 having a molecular weight of about 5 kDa in Piti-Six (PT-6) were obtained.

In the base state, PITC binds to the N-terminal amino group of the protein to form a PTC-polypeptide. The peptide bonds of the N (-)-terminal PTC (residue) residues are cleaved by acid hydrolysis. This results in the formation of en minyl (n-1) peptides and ATZ-amino acids. The unstable amino acid, ATZ-amino acid, is converted into a more stable form, PTH-amino acid. The final product, PTH-amino acid, is analyzed using HCPL (HPLC). As a result, Piti-Five (PT-5) was associated with Solanium Lycopiscomb (tomatofolate stress protein in tomato), Solanium tubulosome (fruit-maturated protein), and Solanium chismania (water deficient stress protein). I showed same sex. PT-6 was homologous to the c-terminal portion of a kunitz-type protease inhibitor or a serine protease inhibitor.

Comparative Example 1 Preparation of Common Potato Protein Extract

In the same manner as in Example 1, the protein was extracted from the potato, dried under reduced pressure and dried to obtain a dry powder.

<Experimental Example 1> Determination of the hamam activity of the protein extracted from potato yam 1

end. Experimental Materials and Experimental Cell Lines

As a test material, protein extracts of potatoes prepared in Examples 1, 2, and Comparative Example 1 were used, and experimental cell lines were obtained from a Korean cell line bank under constant conditions (temperature: 37 ° C., carbon dioxide: 5%). Fully grown human skin cancer cells, G-361 (G-361) and human gastric cancer cells (AGS) were prepared.

I. Experiment method

Dilute the cell line to 2 X 10 ³ for each well of a 96-well plate, and dispense 100 microliters of Media-RPM-1641 (RPMI-1640) including cancer cells to the plate and attach the cell line in a 37 ° C incubator. Incubate until Increased concentrations of acetonitrile buffer obtained in Examples 1 and 2 (10-15%, 15-20%, 20-25%, 25-30%, 30-60% after cell line attachment) After eluting with), the peptide of each compartment and the protein extract of potato prepared in Comparative Example 1 were put in 100 micrograms per well, and incubated in a 37 ° C. incubator for 24 hours. And 50 micrograms of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide (3- (4,5-dimethyl-2-thiazolyl) in each well. ), 2,5-diphenyl-2H-tetrazolium bromide) solution was added and incubated for 4 hours at 37 ℃ incubator. The absorbance of each well was measured at 570 nm (nm) using an ELISA reader and shown in Tables 1 and 2.

All. Experiment result

Human skin cancer cell line G-361 (G-361) and human gastric cancer cell line AGS (AGS) two cell lines are obtained from the Gochu Valley potato prepared in Example 1 according to the present invention and peptide obtained in Example 2 It was confirmed that it is inhibited by.

As can be seen in Table 1 and Table 2, Example 1 according to the present invention shows an inhibitory effect on human skin cancer and gastric cancer cells, but it can be seen that it has anticancer activity, Comparative Example 1 is a general potato Unlike the anti-cancer effects that have been given by therapy, it can be seen that they show little effect.

Therefore, the protein extract extracted from the new varieties of Golan valley ( Solanum tuberosum L., cv. Gogu valley) is very effective in anticancer activity unlike the other potatoes.

<Experiment 2> Determination of the hamam activity of the protein extracted from potato yam 2

A peak of 20-25% acetonitrile concentration obtained in Example 2 was obtained by reverse phase ultra-fast liquid chromatography, and the peaks were measured in the same manner as Experimental Example 1 in activity of gaseous cancer cells (AGS). 7 is shown.

As can be seen in Figure 7, the peptide fraction represented by SEQ ID NO: 1 having a molecular weight of about 7 kDa (No. 5) and the peptide fraction represented by SEQ ID NO: 2 having a molecular weight of about 5 kDa (No. 6) It showed anticancer activity.

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Figure 1 shows a real picture of a potato potato Solanum tuberosum L. (cv. Gogu valley) according to the present invention.

Figure 2 is a tricine electrophoresis picture of each step for separating only a small molecular weight peptide of the protein extract of potato yam according to the present invention.

Lane 1: Total Protein Extracted from Potato Valley Potatoes

Lane 2: Sample obtained by ion-exchange chromatography

Lane 3: The peptide after cleavage to molecular weight 10,000 or less is shown.

Figure 3 is a photograph showing the results of performing reversed phase ultrafast liquid chromatography with a peptide having a small molecular weight finally obtained in the protein extract of the sweet potato valley potato according to the present invention.

(a): 10-15% concentration of acetonitrile,

(b): 15-20% concentration of acetonitrile,

(c): 20-25% concentration of acetonitrile,

(d): 25-30% concentration of acetonitrile,

(e): It shows the 30-60% concentration of acetonitrile.

Figure 4 is a photograph of the electrophoresis of each compartment obtained by performing reverse phase ultra high-speed liquid chromatography with proteins and peptides extracted from the sweet potato valley potato according to the present invention. Depending on the concentration of acetonitrile, 10 to 15%, 2 to 15-20%, 3 to 20-25%, 4 to 25-30% and 5 to 30-60% are shown.

FIG. 5 is a profile obtained by performing reverse phase ultra high performance liquid chromatography once again in acetonitrile concentration 20-25%.

FIG. 6 is a photograph showing electrophoresis of each peak 1 to 11 obtained by performing reverse phase ultra-high performance liquid chromatography once again in acetonitrile concentration of 20-25%.

FIG. 7 is a graph showing the results of measuring anticancer activity against gastric cancer cells at each peak obtained by performing reverse phase ultra-fast liquid chromatography in acetonitrile concentration of 20-25% once again.

FIG. 8 shows the results of amino acid sequencing of five times of the peaks obtained by injecting acetonitrile concentration 20-25% in reverse phase ultrafast liquid chromatography, ie, piti-five (PT-5).

FIG. 9 shows the results of amino acid sequence analysis of 6 times of peaks obtained by injecting acetonitrile concentration 20-25% into reverse phase ultrafast liquid chromatography, ie, piti-five (PT-6).

<110> Potato Valley Inc.          KNU-Industry Cooperation Foundation          Industry-Academic Cooperation Foundation, Chosun University <120> Pharmaceutical composition for the anticancer configuring the          extracted protein or peptide from Solanum tuberosum L cv. Gogu          Valley <160> 2 <170> KopatentIn 1.71 <210> 1 <211> 10 <212> PRT <213> Solanum tuberosum <220> <221> PEPTIDE (222) (1) .. (10) <400> 1 Thr Val Ala Ala Gly Ala Tyr Ala Leu His   1 5 10 <210> 2 <211> 10 <212> PRT <213> Solanum tuberosum <220> <221> PEPTIDE (222) (1) .. (10) <400> 2 Ser Asp Asp Gln Phe Cys Leu Lys Val Gly   1 5 10  

Claims (9)

A pharmaceutical composition for treating skin cancer or stomach cancer, comprising extract of Solanum tuberosum L., cv. Gogu valley, which is extracted by adding a protein extraction buffer. delete According to claim 1, wherein the extract is a pharmaceutical composition for the treatment of skin cancer or stomach cancer, characterized in that the formulation is formulated by a formulation selected from liquids, tablets, granules, capsules, suspensions, syrups, ointments or injections. 20 of acetonitrile buffer containing 0.1% Trifluoroacetic acid (TFA) from the extract of Solanum tuberosum L., cv.Gogu valley extracted with protein extraction buffer A pharmaceutical composition for the treatment of skin cancer or gastric cancer containing a peptide having a molecular weight of 10,000 or less eluted in a -25% concentration compartment. delete According to claim 4, wherein the peptide eluted at 20-25% concentration increase of acetonitrile (actronitrile) is a pharmaceutical composition for the treatment of skin cancer or gastric cancer, characterized in that the peptide represented by SEQ ID NO: 1. According to claim 4, wherein the peptide eluted at 20-25% concentration increase of acetonitrile (actronitrile) is a pharmaceutical composition for the treatment of skin cancer or gastric cancer, characterized in that the peptide represented by SEQ ID NO: 2. delete 8. The skin cancer according to any one of claims 4, 6 or 7, wherein the peptide is formulated by a formulation selected from liquids, tablets, granules, capsules, suspensions, syrups, ointments or injections. Or a pharmaceutical composition for treating stomach cancer.

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