KR100864112B1 - Manufacture of ruminally protected fat supplemented with amino acids - Google Patents

Abstract

The present invention relates to a method for preparing protective fat for feed, and more particularly, to prepare a protective fat through a saponification reaction by mixing metal materials with vegetable fats and oils; Pulverizing the protective oil to powder; And mixing and stirring the powdered protective oils, amino acids, and binding coating agents, followed by extrusion, wherein the binding coating agents are any one or more selected from the group consisting of hardened oil, extremely hardened oil, palmitic acid and stearic acid. The present invention relates to a method for preparing rumen protective fat to which amino acids are added.

According to the manufacturing method of the present invention, the effective supply of amino acids and simultaneous supply of protective fats are also possible.

Rumen, protected fat

Description

Manufacture of ruminally protected fat supplemented with amino acids

Figure 1 shows the digestion rate investigation process for each digestive tract using a mobile bag.

Figure 2 shows the rumen protective fats added with amino acids for each oil type.

Figure 3 shows the post-rumen (fourth place + small intestine) and the total digestive tract digestibility (%) of the rumen protected fats added amino acids by oil type.

Figure 4 shows the crude fat digestibility (%) in the total digestive tract of the rumen protected fats added with amino acids by oil type.

The present invention relates to a method for preparing protective fat for feed, and more particularly, to prepare a protective fat through a saponification reaction by mixing metal materials with vegetable fats and oils; Pulverizing the protective oil to powder; And mixing and stirring the powdered protective oils, amino acids, and binding coating agents, followed by extrusion, wherein the binding coating agents are any one or more selected from the group consisting of hardened oil, extremely hardened oil, palmitic acid and stearic acid. The present invention relates to a method for preparing rumen protective fat to which amino acids are added.

The productivity of ruminants can be substantially improved through the strategic supply of energy and protein (amino acids), and vegetable fats as an energy source for ruminants due to increased energy density in the feed and the safety of adding animal by-products to livestock feed. There is an increasing interest in protected fats.

Protected fat has three times higher net energy than corn, so it can efficiently increase the energy density of the feed. Since it is insoluble at pH 6 ~ 7, it is insoluble in the rumen and dissolves in the low pH and small intestine. Absorbed and used. In other words, the feeding of protected fats for beef cattle and cows can increase the energy density of the feed without adverse effects on the rumen environment. It is possible to regulate fat synthesis.

On the other hand, NRC (2000) reported that both the amount and ratio of amino acids available in beef cattle is important in obtaining the maximum energy for optimal daily gain, and the amino acid source used in the small intestine of beef cattle and cows is largely the microbial body protein. Derived amino acids and ruminant undigested protein (UIP) -derived amino acids can be classified, and until recently, a variety of UIP feed has been used as an amino acid source for beef cattle.

In recent years, however, bypass proteins (amino acids) from fertilized cattle and high-performance cows have been restricted in accordance with restrictions on the use of animal by-products (protein and amino acid raw materials) in feed formulations for ruminants in Europe (94/381 / EC, 95/60 / EC). Difficulties in meeting requirements are increasing, and in countries other than Europe, the use of animal by-products for meeting protein (amino acid) requirements of ruminants is increasingly limited in terms of policy and consumer preference.

Therefore, interest in vegetable protein sources that can supply enough essential amino acids such as methionine and lysine at the same time as rumen degradation rate is low, but vegetable protein sources lack lysine supply compared to animal protein supplementation. Corn and corn gluten meal, which are most commonly used, have a low lysine content and low methionine content in the case of blood meal, an animal protein source. In addition, most of the feeds with high UIP content lack one or more essential amino acids, and even if the feeds are mixed, the increase in essential amino acids entering the small intestine is weak.

Thus, methionine and lysine are not only essential nutrients for livestock, but also ruminant protection of methionine and lysine can improve the feed efficiency and fertility of beef cattle. Some techniques for suppressing have been devised.

Amino acid-coating chelate method and encapsulation method using pH-sensitive polymers are currently available.Available products include Smartamine M ™ coated with 2-vinylpyridine-co-styrene and stearic acid. There are Mepron® M85 coated with ethylcellulose and stearic acid and METHIO-BY coated with lipid matrix, but there are disadvantages of using an excipient and increasing the price of the coating material in the coating process.

In addition, since none of the commercially available protected amino acid preparations supply methionine and lysine at the same time, these protective methionine have to be purchased and used separately when lysine is additionally supplied. Almost no situation.

The present invention to solve the above problems, by coating the methionine and lysine with vegetable protective fat for the purpose of simultaneously supplying the restriction amino acids methionine and lysine and energy to the ruminant, especially beef cattle and cows by inhibiting degradation in the rumen Efficient use of methionine and lysine and efficient energy through protecting fats, which are essential for proper feeding of these breeds, but have not been able to be easily decomposed in the rumen to achieve optimal feeding effects. The present invention is to provide a method for preparing a supplementary dietary supplement of ruminant protective fat supplemented with amino acids (methionine and lysine) that can improve productivity by improving nutrient utilization efficiency in finishing beef or high-capacity cows through controlling fatty acid supply.

Other objects and advantages of the invention will be described below and will be appreciated by the practice of the invention. In addition, the objects and advantages of the present invention can be realized by means and combinations indicated in the claims.

In order to achieve the above object, the present invention comprises the steps of preparing a protective oil through a saponification reaction by mixing a metal material with vegetable oils and fats; Pulverizing the protective oil to powder; And mixing and stirring the powdered protective oils, amino acids, and binding coating agents, followed by extrusion, wherein the binding coating agents are any one or more selected from the group consisting of hardened oil, extremely hardened oil, palmitic acid and stearic acid. Provided is a method for preparing rumen protected fat to which amino acids are added.

Vegetable oil is any one or more selected from the group consisting of cottonseed oil, soybean oil, rapeseed oil, palm oil, coconut oil, olive oil, sunflower oil, peanut oil, palm kernel oil, grape seed oil and corn oil.

The metal material is any one or more selected from the group consisting of sodium hydroxide, potassium hydroxide, triethanolamine, monoethanol amine, sodium carbonate, calcium hydroxide, calcium chloride, magnesium hydroxide and zinc hydroxide.

Amino acids include lysine, methionine, glycine, alanine, valine, leucine, isoleucine, threonine, serine, cysteine, cystine, aspartic acid, asparagine, glutamic acid, diyotyrosine, arginine, histidine, phenylalanine, tyrosine, tryptophan, proline and oxyproline Any one or more selected from the group consisting of are used.

The binder coating agent is bivalent soap such as biswax, PEG, surfactant, calcium stearate, zinc stearate, magnesium stearate, sodium monohydrate soap such as potassium soap, hardened oil, extremely hardened oil, palmitic acid, stearic acid, etc. Any one or more selected from the group consisting of fats and oils and fatty acids are used.

It is composed of 25 ~ 75% by weight of protective oil, 5 ~ 75% by weight of amino acid, and the remaining binding coating agent.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms or words used in the present specification and claims should not be construed as being limited to the common or dictionary meanings, and the inventors should properly explain the concept of terms in order to best explain their own invention. Based on the principle that can be defined, it should be interpreted as meaning and concept corresponding to the technical idea of the present invention.

Therefore, the embodiments described in the specification and the drawings shown in the drawings are only the most preferred embodiments of the present invention and do not represent all of the technical idea of the present invention, various equivalents that may be substituted for them at the time of the present application It should be understood that there may be water and variations.

<Example>

Protective oils were prepared by mixing and reacting vegetable oils with metals such as calcium hydroxide, and then pulverizing the protective oils to select powdery protective oils of 10 mesh or less. A predetermined proportion of amino acids were added to the selected protective oil, followed by mixing and stirring to prepare a rumen protective fat to which the amino acid was encapsulated and coated with amino acids.

Example 1

A protective oil was prepared by mixing and reacting 437 wt% of cottonseed oil with 59 wt% of calcium hydroxide. 10 wt% of the liquid cured oil was added to the prepared protective oil and 65 wt% of the oil was mixed well, and then 25 wt% of the amino acid was mixed well to prepare a ruminant protective fat to which the amino acid was added using an extruder. At this time, methionine and lysine in the amino acid was used in a mixture of 1: 2 ratio. The proportion of amino acids in the rumen protected fats added with the amino acids described above is 25.0%.

Example 2

437% by weight of soybean oil was mixed with 59% by weight of calcium hydroxide to prepare a protective oil. 10 wt% of the liquid cured oil was added to the prepared protective oil and 65 wt% of the oil was mixed well, and then 25 wt% of the amino acid was mixed well to prepare a ruminant protective fat to which the amino acid was added using an extruder. At this time, methionine and lysine in the amino acid was used in a mixture of 1: 2 ratio. The proportion of amino acids in the rumen protected fats added with the amino acids described above is 25.0%.

Example 3

Protective oil was prepared by mixing 59% by weight of calcium hydroxide with 441% by weight of rapeseed oil. 10 wt% of the liquid cured oil was added to the prepared protective oil and 65 wt% of the oil was mixed well, and then 25 wt% of the amino acid was mixed well to prepare a ruminant protective fat to which the amino acid was added using an extruder. At this time, methionine and lysine in the amino acid was used in a mixture of 1: 2 ratio. The proportion of amino acids in the rumen protected fats added with the amino acids described above is 25.0%.

Example 4

A protective fat was prepared by mixing and reacting 59 wt% of calcium hydroxide with 424 wt% of palm oil. 10 wt% of the liquid cured oil was added to the prepared protective oil and 65 wt% of the oil was mixed well, and then 25 wt% of the amino acid was mixed well to prepare a ruminant protective fat to which the amino acid was added using an extruder. At this time, methionine and lysine in the amino acid was used in a mixture of 1: 2 ratio. The proportion of amino acids in the rumen protected fats added with the amino acids described above is 25.0%.

On the other hand, to examine the digestibility and safety of rumen protected fats supplemented with amino acids prepared to provide both rumen protection and protective fats of methionine and lysine in order to increase the nutrient availability of ruminants and produce high quality livestock products, rumen and small intestine cannula A test was conducted to investigate the nutrient degradation rate of each digestive organ in castrated Korean cattle.

0.5 g of the ruminant protective fat added with the amino acid prepared in the above Example was placed in each mobile bag (4 cm × 3 cm) and suspended for 9 hours in the rumen of the test shaft subjected to the rumen cannula, followed by washing with a washer for 5 minutes. It was. The mobile bags for measuring the rumen bypass were dried for 48 hours at 39 ° C., and then weighed after cooling to measure the rumen bypass rate. The remaining mobile bags were stored for rumen digestion measurement experiments. After 9 hours of ruminant digestion, the mobile bags were reacted for 3 hours in artificial gastric juice (39 ° C.) containing HCl and pepsin, and then washed with a washer for 5 minutes. The mobile bags for the 4th digestibility was dried for 48 hours at 39 ℃ to measure the weight after cooling to calculate the 4th digestibility, and the remaining mobile bags were stored for the 4th lower organ digestibility measurement experiment. The mobile bags, which had undergone the fourth digestibility test, were injected into the test shaft of the duodenal cannula at 20 minute intervals, and then the mobile bags excreted in minutes from 36 hours were collected and washed with a washer for 5 minutes. The washed mobile bags were dried for 48 hours at 39 ° C., and cooled to calculate the small intestinal digestibility after cooling (see FIG. 1).

Figure 2 shows the rumen protective fats added with amino acids for each oil type.

<Table 1> Dry matter digestibility of digestive organs of rumen protected fats added with amino acids by oil type (%)

division Rumen 4th Intestine all Example 1 24.39 1.66 64.45 90.50 Example 2 27.70 2.45 61.26 91.41 Example 3 27.89 0.71 54.55 83.15 Example 4 29.10 2.53 63.44 95.07

In the test of the digestive organ digestibility of rumen protected fats supplemented with amino acids by oil type using the castrated Hanwoo with rumen and small intestine cannula, the rumen, fourth place, small intestine and total digestibility were 24.4-29.1% and 0.7-2.5%, respectively. For example, 54.6-64.5% and 83.15-95.07% confirmed that much of the rumen was able to migrate to the lower digestive tract, avoiding degradation. In particular, Examples 1 and 4 were higher in small intestine and total digestibility than in Examples 2 and 3, and in comparison between the two examples, the total digestibility was about 5% higher in Example 4 than in Example 1. Example 4 was found to be superior to Example 1 (see Figure 3).

Ruminant protective fat supplemented with amino acids by oil type is digested and absorbed in livestock and used in body tissues. The post-rumen digestion rate is 55.3-66.1%, especially in Examples 1 and 4. It was confirmed that it was excellent, and the digestibility of the entire digestive tract of amino acid-protected fats was 74.8-93.0%, and it was confirmed that a large amount of protected fats could be absorbed in the small intestine and used as an energy source for livestock. In particular, it was confirmed that Example 4 is higher in fat digestibility and economical than other examples (see Fig. 4).

The rumen protected fats added with the amino acids prepared by the method of the present invention are capable of simultaneously supplying the protected fats together with the effective coating of the amino acids. This simultaneous supply of protected fats and restriction amino acids (methionine and lysine) is the best way to supply energy and amino acids absorbed by the small intestine at the same time. There is an advantage that the productivity of the cow can be improved.

When the protective fat of the present invention is fed to the ruminant shaft, methionine and lysine are protected in the rumen, so it is possible to add them to the feed and use them as a feed, thereby producing high quality livestock products through the simultaneous feeding effect of amino acids and protective fat. .

Claims (8)

delete delete delete delete delete delete Mixing a vegetable material with vegetable oil to prepare a protective oil through a saponification reaction; Pulverizing the protective oil to powder; And To include the step of extruding after mixing and stirring the protective oils, amino acids and binding coating agent in the powder form, The binder coating agent is a method of producing a ruminate protective fat with an amino acid, characterized in that any one or more selected from the group consisting of hardened oil, extreme hardened oil, palmitic acid and stearic acid. delete

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