KR100851289B1 - Method for producing fermented mixed grains using living effective microorganisms and fermented mixed grains produced by the same - Google Patents

Abstract

Fermented miscellaneous cereals containing effective microorganisms and its preparation method are provided to enable consumers to allow the effective microorganisms to survive even after boiling the fermented miscellaneous rice. A preparation method of fermented miscellaneous cereals containing effective microorganisms comprises the steps of: (a) making a culture solution with effective microorganisms including Lactobacillus casei or Lactobacillus plantarum, Saccharomyces cerevisiae, Bacillus subtilis, and Streptomyces griseus; and (b) adding the culture solution to miscellaneous cereals, and then fermenting them at 20~40°C for 3~14day. The miscellaneous cereals are composed of two kinds selected from the group consisting of glutinous rice, glutinous brown rice, glutinous black rice, glutinous Italian millet, millet, glutinous Indian millet, barley, glutinous barley, mung beans, red beans, white beans, black beans of which the flesh is green, black beans, yellow beans, sesame seeds, perilla seeds, black sesame seeds, and Rhynchosia Nulubilis.

Description

Method for producing fermented mixed grains using living effective microorganisms and fermented mixed grains produced by the same

The present invention comprises the steps of preparing a useful microbial culture solution for the production of fermented grains consisting of lactic acid bacteria, yeast, Bacillus subtilis and actinomycetes and adding the useful microbial vegetation to the grains for 3 to 14 days at 20-40 ℃. The present invention relates to a method for preparing fermented grains having useful microorganisms after cooking and to fermented grains prepared by the method.

Useful microorganisms (lactic acid bacteria, yeast, etc.) are beneficial to the human body and have various physiological activities. Among them, lactic acid bacteria refers to microorganisms that break down sugars such as glucose to produce lactic acid, and coexist relatively well with other microorganisms. Lactic acid produced by lactic acid fermentation prevents the growth of pathogens and harmful bacteria and is used in food production. In addition, it is an important microorganism that lives in the intestines of mammals to prevent abnormal fermentation by various bacteria and is also used as a dressing agent. It is a gram-positive bacterium and is anaerobic or anaerobic. There is no mobility and most are catalase negative, and growth requires various vitamins, amino acids, certain types of peptides, and the like.

Yeast is the mother of fermentation, which is essential for brewing and baking. It is a group of fungi and mushrooms, but it is a generic term for single-celled organisms without mycelia and without photosynthesis or motility. Most do not live in soil and grow in high concentrations of sugar such as flower glands or fruit surfaces. Many have the ability to ferment sugars to produce ethanol and carbon dioxide. This property is used for beer production and bread fermentation. Yeasts contain more than 50% protein and are a rich source of vitamins B ₁ and B ₂ , nicotinic acid and folic acid (folic acid). Brewed yeast is often taken as a vitamin supplement. Therefore, yeast is a kind of fungus in the classification of microorganisms and is indispensable for human life.

Rice is heated to make rice or powder is used to process rice cakes, bread, noodles, and confectionery, and the conventional technique is to produce liquor by fermentation with yeast, yeast, and the like. As a conventional technique for preparing rice using microorganisms, Korean Laid-Open Patent Publication No. 2005-121109 relates to a method for producing mushroom cultured rice comprising inoculating the cultured rice with mushroom fungi and incubating and drying the rice. Patent No. 723316 discloses a fermented rice culture of Bokryeong mycelium cultured by inoculating Bokryeong mycelium on sterilized rice.

However, these techniques are locally fermented rice using a single microorganism or mycelium, and stays in that fermented grains are not made using useful microorganisms that can be lived by boiling water, which is the same condition as cooking.

The present invention is to solve the above-mentioned conventional problems, the technology to which the present invention belongs to the use of useful microorganisms that can survive even boil water to survive even cooked multigrain rice. The present invention is to develop a fermented grains in which useful microorganisms are completely fermented by addition of two or more useful microorganisms, and even after cooking.

The technology to which the present invention belongs to achieve the above object is mainly to invent fermented grains using useful microorganisms composed of lactic acid bacteria and yeast, through the endless experiments and studies of the present inventors based on the principle that the lactic acid bacteria and yeast symbiosis together Using the selected useful microbial combination, fermentation of the whole grains allows the useful microorganisms to remain even after cooking.

To this end, the present invention comprises the steps of preparing a useful microorganism culture medium for the production of fermentation grains consisting of lactic acid bacteria, yeast, Bacillus subtilis and actinomycetes and adding the useful microorganisms to the grains for 3 to 14 days at 20-40 ℃. The present invention relates to a method for preparing fermented grains in which microorganisms are alive after cooking.

In addition, the present invention provides a fermented grains that are useful microorganisms after cooking prepared by the method for producing fermented grains of the present invention.

Fermented grains fermented completely by adding useful microorganisms have useful microorganisms even when cooked. If microbes eat live rice, they can promote digestion and produce antibiotics in the body to improve various immune functions. In addition, it inhibits the growth of common decaying bacteria and pathogens to inhibit the growth of decay and pathogenic bacteria. In addition, antimicrobial substances increase the shelf life of fermented products. In particular, it is convinced that the continuous use of useful microorganisms can be consumed as rice, thereby enhancing national health and competitiveness in rice imports. In particular, fermented grains contain a large amount of useful microorganisms and can be easily used for special nutritional products such as patient foods and healthy foods. Fermented brown rice contains considerable functionality and is expected to contribute to national health in the future. In the case of fermented grains, microorganisms that are useful even when cooked are alive and can be spotlighted as the best nutritional foods.

In order to achieve the object of the present invention, the present invention comprises the steps of preparing a useful microbial culture for the production of fermented grains consisting of lactic acid bacteria, yeast, Bacillus subtilis and actinomycetes; And it provides a method for producing fermented grains of the useful microorganisms after cooking comprising the step of fermenting for 3 to 14 days at 20 ~ 40 ℃ by adding the useful microbial culture to the grains.

In the method according to an embodiment of the present invention, in the useful microbial culture for the production of fermented grains, lactic acid bacteria is Lactobacillus casei ( Lactobacillus casei ), Lactobacillus plantarum , yeast may be Saccharomyces cerevisiae , Bacillus subtilis may be Bacillus subtilis , more preferably May be Bacillus subtilis subsp.spizizepii , and actinomycetes may be, but are not limited to, Streptomyces griseus .

Preferably, the useful microorganisms are Lactobacillus casei , Lactobacillus plantarum , Saccharomyces cerevisiae , Bacillus subtilis subspecies sp. subsp.spizizepii ) and Streptomyces griseus , but is not limited thereto.

Conditions and media for fermentation by adding useful microbial cultures to the grains may be those known in the art.

In the method of producing fermented grains according to an embodiment of the present invention, the grains are glutinous rice, brown rice glutinous rice, waxy black rice, green tea, millet, sorghum, barley, waxy barley, mung bean, red bean, white tae, seotae, black tae, yellow tae, sesame, perilla It may be two or more selected from the group consisting of black sesame and weak soybeans, but is not limited thereto.

In addition, the present invention provides a fermented grains that are useful microorganisms after cooking prepared by the method for producing fermented grains of the present invention. The fermented grains prepared above are useful microorganisms alive even after cooking (see Table 2), and the useful microorganisms can enhance rice's various immune functions by promoting digestion and producing antibiotics in the body. The fermented grains of the useful microorganisms can be prepared with glutinous rice, brown rice glutinous rice, waxy black rice, tea, millet, sorghum, barley, green barley, mung bean, red bean, white bean, frosted tree, black bean, yellow bean, sesame, perilla, black sesame, medicinal beans, etc. However, the present invention is not limited thereto.

In addition, the present invention provides a grain processed product manufactured from the fermented grains as a raw material. The processed grains manufactured from the fermented grains as raw materials may be, but are not limited to, noodles, rice cakes, breads, confectionary, and the like.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example

Preparation of useful microorganisms (θ-1, theta circle)

The following combination of useful microorganisms, through continuous research and experiments of the present inventors, through the fermentation process in rice to maintain a symbiotic relationship and create a safe vegetation group, highlighting that the optimal microbial combination that a large number of useful microorganisms exist even after cooking I will.

1) Lactobacillus and yeast are purchased from the GenBank as follows.

Lactobacillus casei

Lactobacillus plantarum

Yeast ( Saccharomyces cerevissiae )

2) Bacillus subtilis and actinomycetes are separated from the rice hulls and rice bran into individual progenitors by the following method.

Bacillus subtilis subsp.spizizepii

Actinomycetes ( Streptomyces griseus )

Place the eco-friendly rice husk on the medium prepared by mixing about 10% by weight of brown sugar in the gourd rice, cover the cloth, and incubate for 1 week while spraying the water suitable for growth twice a day at a temperature of about 25 ℃ to produce Bacillus subtilis and actinomycetes Then, it was inoculated again in a medium in which rice bran and brown sugar were mixed in a 50:50 weight ratio, and were used as individual progenitors after culturing for one week at 25 ° C.

Preparation of useful microorganisms (θ-1, theta circle)

The selected strains according to the present invention are inoculated through sterile platinum teeth in agar medium prepared by dissolving in distilled water in a clean bench, and the inoculated individual bacteria are grown in incubators according to their respective growth temperatures. Individual bacteria inoculated into the solid medium are inoculated into each liquid medium (PD broth-yeast; LB broth-Bacillus, actinomycetes; MRS broth-lactic acid bacteria), and the liquid medium is dissolved in boiling water and bottled in foil. After autoclave sterilization in autoclave for 15 minutes at 121 ℃, sterilize and cool through clean lamp with UV lamp and blowing and inoculate solid medium. The inoculated bottle is sealed with foil, resealed with Parafilm, and put into a shaker incubator for 1 to 3 days. The individual microorganisms produced as described above were mixed with water and starch syrup (1 to 35% by weight) in a stainless liquid fertilizer or fermenter, and incubated for about 3 days with a bubbler in operation. -1) is used.

All of the prepared individual bacteria may be purchased and used, and it is preferable to newly purchase and use the seed from the genetic bank after about six months through the passage of passage for fear of aging and contamination.

Preparation of Fermented Grains

1 to 18% by volume of water and 1 to 100-fold dilutions of the useful microorganism (θ-1) are inoculated by spraying at about 1 to 30% by weight, and fermented at 20 to 40 ° C for 3 to 14 days. .

Example 1 Fermented White Rice

Put 1.5kg into 15% moisture content in a plastic sealed container and apply 10% by weight (150g) of 2% by weight (30mL) of water and the 20-fold dilution of the useful microorganism (θ-1). And inoculated, and fermented at 38 ° C. for 7 days.

Example 2: Fermented Barley

Put 1.5kg into a plastic sealed container with 14% moisture content of the cultivated embryo in the Hanbit Farming Co., Ltd., and spray about 10% by weight (150g) of 9% by weight (135mL) of water and the 20-fold dilution of the useful microorganism (θ-1). And inoculated, and fermented at 38 ° C. for 7 days.

Example 3: Fermented Brown Rice

Put 1.5kg of plastic brown rice into 16% moisture content of Hanbit Farming Co., Ltd. in a plastic sealed container and spray about 10% by weight (150g) of 9% by weight (135mL) of water and 20 times the dilution of the useful microorganism (θ-1). And inoculated, and fermented at 38 ° C. for 7 days.

Example 4: Fermented Grains

1.5 kg of the same weight ratio of white rice, brown rice, glutinous rice, brown rice glutinous rice, waxed black rice, green tea, millet, sorghum, barley, green barley, mung bean, red bean, white bran, and frost tea are placed in a plastic sealed container and 5% by weight (75 mL) of water And inoculated by spraying the useful microorganism (θ-1) 20-fold dilution about 10% by weight (150g), and then fermented at 38 ℃ for 7 days.

Sensory evaluation

The palatability of brown rice was cooked with Bubang Techron LiHom (model name: LJ-NB041), and the best taste for taste, flavor, texture, juiciness and overall palatability was scored by 15 trained sensory inspectors. The sensory evaluation was performed using a 7-point symbol scale (Stone & Sidel. Sensory evaluation practices. Academic press Inc., New York, USA, p. 45, 1985).

Table 1. Comparison of Sensory Characteristics of Plain and θ-1 Fermented Brown Rice

Items Treatments Plain Brown Rice θ-1 fermented brown rice Taste 5.23 5.35 Flavor (flavor) 5.08 5.12 Tenderness 4.50 5.21 Juiciness 5.15 5.28 Palatability 4.99 5.24

RESULTS: The θ-1 fermented brown rice according to the present invention was found to have an excellent sensory evaluation compared to ordinary brown rice in overall palatability including taste, flavor, texture and juiciness.

Useful Microbial Survival

In order to measure the microbial bacterial count, yeast was used YM agar medium (Difco, MI, USA), lactic acid bacteria was used MRS medium (Difco, MI, USA), 1 mL of 100-fold dilution in the sample diluted to 10 ⁰ -10 ⁹ Each was dispensed into YM agar medium and MRS medium, respectively, and subjected to standard agar culture (Ministry of Health and Welfare. Offical book of foods, experimental methods. Ministry of Health and Welfare, Korea. P 94. 1997). Colonies were measured after YM agar medium (yeast) was cultured at 24-48 hours at 25 ° C. and MRS medium (lactic acid bacteria) at 30 ° C. for 64 hours. Table 2 below shows the useful microbial survival results.

Table 2. Comparison of useful microbial survival rates after cooking of general cereals and θ-1 fermented cereals

Useful Microorganisms Cereals General Rice Fermented Rice General Grains Fermented Grains Lactobacillus - 3.7 × 10 ³ CFU / mL - 5.6 × 10 ² CFU / mL leaven - 7.2 × 10 ² CFU / mL - 4.8 × 10 ¹ CFU / mL

RESULTS: Lactobacillus and yeast did not survive in normal grains, but useful microorganisms survived in θ-1 fermented grains.

Table 3.Comparison of Component Analysis for Plain and Fermented White Rice

Item＼Treatments protein(%) moisture(%) Amylose (%) Plain white rice 7.0 16.5 18.7 Fermented white rice 6.8 16.6 18.9

RESULTS: As a result of the component analysis, the content of amylose, which indicates that the fermented grains influenced the taste of rice, was slightly higher than that of ordinary white rice, indicating that the texture changed well.

Table 4. Comparison of Component Analysis for Plain and Fermented Brown Rice

Item＼Treatments protein(%) moisture(%) Amylose (%) Fatty acid (mg) Plain Brown Rice 8.5 11.6 18.4 14.9 Fermented Brown Rice 7.9 12.6 18.5 12.8

RESULTS: As a result of component analysis, fermented brown rice showed lower levels of protein and fatty acids than normal brown rice, and amylose content was slightly higher than that of normal brown rice, indicating good flavor and texture.

Antioxidative effect

Antioxidant effect test was used DPPH (1,1-diphenyl-2-picrylhydrazyl) to measure the free radacal scavenging activity by varying the concentration of the solvent (water, ethanol) extract. That is, 0.6 ml of extract was added to 2.4 ml of 4.0X10 ^-4 M DPPH solution, shaken for 10 seconds with a vortex mixer, left at room temperature for 30 minutes, and the absorbance was measured at 517 nm. Activity was shown. At this time, ascorbic acid, an antioxidant, was added to the sample concentration for the comparison of activity and measured by the same method.

Table 5. Comparison of Antioxidant Effects on Plain White Rice, Brown Rice, Fermented Brown Rice, and Fermented Grains

Item＼ Treatment DPPH (%) H ₂ O Ethanol Plain white rice 6.03 4.86 Brown rice 29.67 30.11 Fermented rice 10.69 10.37 Fermented embryo 12.03 11.89 Fermented germinated brown rice 30.36 31.28 Fermented Brown Rice 30.23 31.37 Fermented wheat 28.36 26.74 Fermented barley 32.46 31.22 Fermented Grains 27.95 28.46

RESULTS: The antioxidant effects of fermented embryonic rice, fermented brown rice, fermented brown rice, fermented wheat, fermented barley and fermented grains were higher than those of white rice and brown rice. This is because the antioxidant effect was increased by fermentation.

The features and advantages of the present invention will become more apparent from the following detailed description based on the accompanying drawings. Prior to this, the terms or words used in the present specification and claims are defined in the technical spirit of the present invention on the basis of the principle that the inventor can appropriately define the concept of the term in order to explain his invention in the best way. It must be interpreted to mean meanings and concepts.

Claims (3)

Preparing a useful microbial culture consisting of lactic acid bacteria, yeast, Bacillus subtilis and actinomycetes; And adding the useful microorganism culture medium to the grains for fermentation at 20 to 40 ° C. for 3 to 14 days. According to claim 1, wherein the grains are selected from the group consisting of glutinous rice, brown rice glutinous rice, waxy black rice, tea, millet, waxy water, barley, waxy barley, mung bean, red bean, white tae, frosted, black tae, yellow tae, sesame, perilla, black sesame and medicinal beans Fermented grains production method, characterized in that two or more. Fermented grains containing the useful microorganisms after cooking prepared by the method of claim 1 or 2.