KR100835024B1 - producing method of tissue calture and changing botanically make use to silver NANO. - Google Patents

Abstract

In the present invention, 50 ml / l of silver nano silver solution and 1 ml / l of π Water (piwater), 3 g / l of activated carbon, 1.0 mg / l of NAA, and 0.1 mg / l of Kinetin were added to MS Media. During the weekly cancer cultivation, the median with silver nano-silver solution reduced the contamination rate by 92%, and the plant growth rate was increased by 63% in MS Media with 1 ml / l of π Water. After 5 days of rotation (shaking) incubated in Colchicine solution, the plant was converted from diploid to tetraploid, and then p-Dimethylaminobenzaldehyde [(CH3) 2NC6H4CHO] was added to the growth medium at a concentration of 0.15%. After 20 days of incubation, PLBs of various colors are produced, and the generated PLBs are selected to obtain various and excellent genetically modified plants.

Silver nanoparticles; One billionth of nanoparticles. Reagent; p-Dimethylaminobenzaldehyde [(CH3) 2NC6H4CHO]. π Water. Colchicine. MS Media

Description

Producing method of tissue calture and changing botanically make use to silver NANO.

1: PLB production step in the growth medium

FIG. 2: Adding p-Dimethylaminobenzaldehyde [(CH ₃ ) ₂ NC ₆ H ₄ CHO] at a concentration of 0.15% to the growth medium to produce PLBs of various colors.

Figure 3: Mutant plants of small species (monolitic) with chimera on the leaves.

Figure 4: Mutant plants of large species with chimera on the leaves.

◎ Stage 1: PLB production stage by cultivation

In-flight mass breeding has been carried out by aseptic germination and tissue culture of seeds, but the challenge is how to reduce contamination rate, increase survival rate, and reduce whitening.

To compensate for this drawback, 50 ml / l of silver nanosilver solution and 1 ml / l of π Water (piwater), activated carbon 3g / l, NAA 1.0mg / l and Kinetin 0.1mg / l were added to MS Media. After 2 weeks of cancer cultivation, the rate of contamination was reduced by 92% in the media containing silver nanosilver solution, and the growth rate of plants in MS Media with 1 ml / l of π Water was added. Was increased by 63% and bleaching was reduced by more than 50%.

◎ Stage 2: Mutant Plant Production Stage.

Since the expression of mutant plants is about 30% higher than the use of side eggs, choose a negative child, and then make a whole piece tissue of 5㎣ size with Tissu again and dilute 20,000-fold colchicine solution 5 45 ml of MS Media (MS medium) was mixed, and after 5 days of rotation (shaking), the plants were converted from diploid to tetraploid, and the tissue was washed well so that no colchicine solution remained. After 0.01-0.5% of p-Dimethylaminobenzaldehyde [(CH ₃ ) ₂ NC ₆ H ₄ CHO] was added to the growth medium and incubated for 20 days, the most diverse color of PLB appeared at 0.15% concentration. Could.

◎ Step 3: Cultivation of tissue cells.

The chlorophyll-rich and slow-growing PLB in various colored tissues (Tissu) was grown in solid medium for growth and grown as a complete plant. As a result, all the tissues are thick and the plant is dwarfed (aka monofoliate). ,

Chlorophyll-free and fast-growing PLBs were subcultured in growth medium to grow into complete plants. As a result, thick stems, wide leaves, large plants, and large petals blossomed into large plants. Is possible,

The pigmented PLB was subcultured in a growth medium and grown as a complete plant, resulting in leaf-transformed plants of various shapes and colors.

Excellent leaf mutant plants have excellent ornamental value and commercialization, and can be bred.

As observed above, the concentration of 0.15% p-Dimethylaminobenzaldehyde [(CH ₃ ) ₂ NC ₆ H ₄ CHO] causes the most somatic mutation, the loss of the plastid, resulting in the chromosomal mutation, leaves, stems and petals The chimera phenomenon also appears.

Conventional tissue culture removes organs, tissues and cells from plants and aseptically incubates in a nutrient-containing medium in the cabin (Tube or Bottle) to induce callus or single cell populations or to regenerate a fully functional plant. Means technology.

The rationale for tissue culture can be aggregated into totilotency and plasticity. Totilotency, published by Schleiden (1838) and Schwann (1839), is that cells are the basic units of life, and all living cells in an organism have the ability to develop independently given the appropriate circumstances. Is called totilotency. Plant cells are associated with the growth of plants and have a greater ability to withstand extreme environments than animals' cells, and plants change their metabolism or growth processes to adapt to the environment. The ability of plants to change their growth and metabolism in response to the environment is called plasticity. Totilotency and plasticity are the reasons for the start of plant tissue culture.

Nowadays, tissue culture has been universalized for the purpose of mass production of the same varieties all over the world.

Variant plants that occur during plant tissue culture vary depending on the plant, but the number, duration, and number of subcultures, variances, malformations, and physiological alterations in the explants or mothers are often caused, resulting in good varieties. Disposal at unprofitable companies resulted in huge economic losses.

The present invention is to systematically produce and improve breeding of excellent mutant plants generated during plant tissue culture, to open the way of mass production, to increase the self-sustainability of the flower industry and seedling industry, which depend on imports, It is to improve.

The contents of the present invention for realizing the above object is made as follows.

After organs, tissues and cells are removed from the plant, the plant is converted from diploid to tetraploid using colchicine solution in the cabin (Tube or Bottle).
Growth rate by sterile incubation of silver nanoparticles (silver of more than 99.99% pure silver with nano-sized fine particles) and π-Water (water containing bivalent trivalent iron salt (Fe ₂ Fe ₃ Cl ₅ ) lipid complex) Abolish callus or unicellular populations, increase and decrease bleaching,

0.15% of p-Dimethylaminobenzaldehyde [(CH ₃ ) ₂ NC ₆ H ₄ CHO] was added to the growth medium and cultured for 20 days to produce PLBs of various colors with genetic modification.

According to the shape and shape of the mutated PLB subcultured to each plant, it is an invention to complete the new adult mutant plants.

Large and small flowers, large and small flowers, and the meaning of flower and flower colors are very large, and by applying the present invention, it is possible to reduce contamination rate and bleaching during tissue culture, and to improve plants. Its strong pride and the flower industry contribute to domestic exports by developing and mass-producing and breeding them to realize the excellent value that our flowers and our trees have as biological resources. It should be recognized as one of the industries that can be done.

Claims (2)

Cultivation in a medium containing silver nanoparticles (purity of more than 99.99% pure silver is made of nano-sized fine particles) and π-Water (water containing a bivalent trivalent iron salt (Fe ₂ Fe ₃ Cl ₅ ) lipid complex) And converting the plant from diploid to tetraploid using colchicine solution, and then treating p-dimethylaminobenzaldehyde [(CH3) 2NC6H4CHO] alone or in combination. delete

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