CN107155890A - Beautiful dew rapid propagation in vitro method by explant of blade - Google Patents
Beautiful dew rapid propagation in vitro method by explant of blade Download PDFInfo
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- CN107155890A CN107155890A CN201710429395.0A CN201710429395A CN107155890A CN 107155890 A CN107155890 A CN 107155890A CN 201710429395 A CN201710429395 A CN 201710429395A CN 107155890 A CN107155890 A CN 107155890A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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Abstract
The purpose of the present invention is that the reproduction speed to overcome traditional beautiful dew propagation method to exist is slow, Character instability, the low shortcoming of survival rate；Also for overcoming in forefathers' jade dew Tissue culture assays, inducing clumping bud differentiation rate is low, and the regeneration period is long, the shortcomings of being not suitable for factorial praluction.Invention provides a kind of method for in-vitro rapid propagation of jade dew, beautiful be exposed in the relatively short time is produced a large amount of genetic backgrounds stabilizations, the consistent regeneration plant of plant type.Jade can be made to be exposed at large-scale breeding in the relatively short time in the case where not influenceing maternal plant normal development and breeding, and materials are not subject to seasonal restrictions, and the regeneration period is short, and callus induction rate is high, and differentiation rate is high, and growth conditions are good after plantlet of transplant.It is highly suitable for the quick breeding of beautiful dew Rare Kinds, the cost of average individual plant regrowth is extremely low, is especially suitable for factorial praluction.
Description
Technical field
The invention belongs to flowers propagation method, and in particular to the asexual rapid propagation method of flowers.
Background technology
Jade dew, is Liliaceae, and the category of volume 12, herbaceos perennial, blade meat is plump full.South Africa is originated in, it is resistance to
Arid, can not resist cold, and avoids hot humid and burning sun is exposed to the sun.Jade dew shoot is generally Dan Sheng, after be in gradually all living creatures's shape, fleshy leaf is in compact
Rosette-stape arrangement.Jade dew profile is exquisite compact, and species is enriched, and leaf color is glittering and translucent, is full of variety, deep to be liked by domestic and international flowers
Good person's likes, with the very high market demand and economic value.The Traditional breeding processes of jade dew, use seed propagation more,
Cutting propagation and division propagation.Wherein, cutting propagation and division propagation are vegetative propagation, and the character of plant can be kept stable,
But the breeding cycle is long, output efficiency is relatively low, in addition, seed propagation is generative propagation, character is produced separation, loses female
This merit, and seedling percent is low, factors above all strong influences production efficiency and product quality.Plant group
Knit the i.e. Vitro Plant culture technique of culture, according to the totipotency of plant cell, using the in vitro organ of plant (such as root, stem,
Leaf, stem apex, flower, fruit etc.), tissue (such as forming layer, epidermis, cortex, endosperm) or cell (such as megaspore, microspore, body are thin
Born of the same parents etc.) and protobiont, under the conditions of sterile and suitable synthetic medium and illumination, temperature etc. are artificial, callus can be induced
Tissue, adventitious bud, adventitious root, form the technology of the complete other products of plant or generation with economic value.By plant group
Regeneration plant produced by knitting culture can keep parent's's (explant source plants) excellent except extremely low change is unusual, substantially
Character.Very big progress has been had been achieved for since plant tissue's culture technique invention.It is fast in vitro using plant tissue culture technique
The beautiful dew of speed breeding can produce the neat plant of plant type on a large scale, further meet the market demand.The beautiful dew tissue culture that forefathers are done
Although experiment also has successful precedent, callus inducing clumping bud differentiation rate is low, causes output efficiency low, greatly hinders
The tissue cultures rapid propagation in vitro of beautiful dew does not have market-oriented working condition in the large-scale application of actual production.The present invention
Jade can be made to be exposed within 1 year and realize quick breeding, and reproductive efficiency is high, is especially suitable for factorial praluction.
The content of the invention
The purpose of the present invention is that the reproduction speed to overcome traditional beautiful dew propagation method to exist is slow, and Character instability is survived
The low shortcoming of rate；Also for overcoming in forefathers' jade dew Tissue culture assays, inducing clumping bud differentiation rate is low, and the regeneration period is long, uncomfortable
The shortcomings of closing factorial praluction.A kind of method for in-vitro rapid propagation of beautiful dew is provided, jade is exposed at generation in the relatively short time
A large amount of genetic backgrounds are stable, the consistent regeneration plant of plant type.
The technical solution adopted in the present invention is：
1. explant is inoculated with and callus induction：The young leaflet tablet of beautiful dew plant is taken, is put into sterile super-clean bench, uses
75% (volume/volume) alcohol-pickled 30~60s, after aseptic water washing 2~3 times, pours into rapidly 1.5~3% (quality/body
Product) 8~10min is soaked in liquor natrii hypochloritis, incline liquor natrii hypochloritis, then with aseptic water washing 4~5 times, will handle well
Beautiful dew blade, it is crosscutting into 1cm or so segments；It is seeded in 0.5mg/L containing 6-BA, NAA 0.1mg/L, KT 1mg/L, sucrose
3.0% (mass/volume), agar 6.0% (mass/volume), pH value is intensity of illumination on 5.6~6.0 MS solid mediums
For 1500~2000Lux, daily 12~14 hours of light application time, cultivation temperature at 24 DEG C~28 DEG C, 40~50 days subcultures once,
Differentiation is can induce when yellow green granular callus is grown.
2. the induction of Multiple Buds：Callus is transferred to inductive differentiation medium：MS+KT0.5mg/L+6-BA (0.1~
0.2mg/L) the natural coconut juices (volume/volume) of+NAA (0~0.1mg/L)+IAA (0.1~0.5mg/L)+20.0%, agar
6.0% (mass/volume), pH value is on 5.6~6.0 MS culture medium.Intensity of illumination is 1500~2000Lux, light application time
Daily 12~14 hours, cultivation temperature at 24 DEG C~28 DEG C, 40~50 days subcultures once, sustainable 1 year of the callus differentiation capability
More than.800 plants or so of regrowth can be differentiated from one piece of callus by subculture repeatedly.
3. strong seedling culture：Regrowth goes to the natural coconut juices of 1/2MS+20% (volume/volume), pH value for 5.6~6.0 it is strong
On seedling culture medium, 1500~2000Lux of intensity of illumination, daily 12~14 hours of light application time, cultivation temperature is at 24 DEG C~28 DEG C.
4. Transplantation of Regenerated Plantlets：Strong sprout plant is grown to after 3~4 centimetres, and the tissue culture bottle of strong sprout plant is taken in greenhouse and beaten
Open, strong sprout plant is adapted to greenhouse after 5~7 days, strong sprout plant is taken out from tissue culture bottle, culture medium is cleaned up,
Transplant into 100% vermiculite or red beautiful native cultivation matrix, cultivation matrix needs to sterilize 30~60 minutes by 121 DEG C.7~15
It or so pours once permeable just viable.
The present invention makes the beautiful regeneration plant for being exposed at and largely producing that genetic background is identical, plant type is unified in the relatively short time
Strain.Leafcutting is revealed as explant using jade, collocation and the environment conditioning measure in the external world are cooperateed with by rational culture medium, takes off and divides
Rate is up to 43%, and inducing clumping bud rate is up to 84.1% referring now to prior art culture and improvement more than 15%, blade explant
Multiple Buds are produced after the callus of generation, differentiation, Multiple Buds can differentiate 800 plants or so of regrowth after subculture, regenerated
The survival rate of seedling up to 95%, under control environment transplant survival rate 100%, referring now to prior art improve 20% with
On.It is extensive numerous that the present invention can be such that jade is exposed in the relatively short time in the case where not influenceing maternal plant normal development and breeding
Grow, and materials are not subject to seasonal restrictions, and the regeneration period is short, and callus induction rate is high, and differentiation rate is high, after plantlet of transplant
Growth conditions are good.It is highly suitable for the quick breeding of beautiful dew Rare Kinds, the cost of average individual plant regrowth is extremely low, fits very much
Close factorial praluction.
Embodiment
Below in conjunction with example, the present invention is described further, but present disclosure is not limited only to following 2 realities
Apply example：
Embodiment one：
It is that explant carries out rapid propagation in vitro to select N1 jade dew blades.
1. explant is inoculated with and callus induction：The young leaflet tablet of N1 jade dew plant is taken, is put into sterile super-clean bench, uses
75% (volume/volume) alcohol-pickled 60s, after aseptic water washing 2~3 times, pours into rapidly 3% (mass/volume) hypochlorous acid
8min is soaked in sodium solution, incline liquor natrii hypochloritis, then with aseptic water washing 4~5 times, it is horizontal by the beautiful dew blade handled well
It is cut into 1cm or so segments；It is seeded in 0.5mg/L containing 6-BA, NAA 0.1mg/L, KT the 1mg/L, (quality/body of sucrose 3.0%
Product), agar 6.0% (mass/volume), pH value is on 5.8 MS solid mediums, intensity of illumination is 2000Lux, light application time
Daily 12 hours, cultivation temperature was at 25 DEG C, and 50 days subcultures once, differentiation are can induce when yellow green granular callus is grown.
2. the induction of Multiple Buds：Callus is transferred to inductive differentiation medium：MS+KT0.5mg/L+6-BA 0.1mg/L+
The natural coconut juices of IAA0.1mg/L+20.0% (volume/volume), agar 6.0% (mass/volume), pH value is 5.8 MS culture mediums
On.Intensity of illumination is 1800Lux, daily 12 hours of light application time, cultivation temperature at 25 DEG C, 45 days subcultures once, the callus point
Sustainable more than 1 year of change ability.820 plants of regrowth can be differentiated from one piece of callus by subculture repeatedly.
3. strong seedling culture：Regrowth goes to the natural coconut juices of 1/2MS+20% (volume/volume), and pH value is trained for 5.8 strong sprout
Support on base, intensity of illumination 2000Lux, daily 12 hours of light application time, cultivation temperature is at 25 DEG C.
4. Transplantation of Regenerated Plantlets：Strong sprout plant is grown to after 3~4 centimetres, and the tissue culture bottle of strong sprout plant is taken in greenhouse and beaten
Open, strong sprout plant is adapted to greenhouse after 5 days, strong sprout plant is taken out from tissue culture bottle, culture medium is cleaned up, transplant
Into 100% vermiculite culture matrix, vermiculite needs to sterilize 30~60 minutes by 121 DEG C.Pour once within 15 days or so and permeable just may be used
Survive.Embodiment two：
It is that explant carries out rapid propagation in vitro to select OB1 jade dew blades.
1. explant is inoculated with and callus induction：The young leaflet tablet of OB1 jade dew plant is taken, is put into sterile super-clean bench,
With 75% (volume/volume) alcohol-pickled 45s, after aseptic water washing 2~3 times, 1.5% (mass/volume) is poured into rapidly secondary
10min is soaked in sodium chlorate solution, incline liquor natrii hypochloritis, then with aseptic water washing 4~5 times, by the beautiful dew leaf handled well
Piece, it is crosscutting into 1cm or so segments；It is seeded in 0.5mg/L containing 6-BA, NAA 0.1mg/L, KT the 1mg/L, (matter of sucrose 3.0%
Amount/volume), agar 6.0% (mass/volume), pH value is on 6.0 MS solid mediums, intensity of illumination is 1500Lux, light
Daily 14 hours according to the time, cultivation temperature is at 24 DEG C, and 50 days subcultures once, can induce point when yellow green granular callus is grown
Change.
2. the induction of Multiple Buds：Callus is transferred to inductive differentiation medium：MS+KT0.5mg/L+6-BA0.1mg/L+
The natural coconut juices of NAA0.1mg/L+IAA0.1mg/L+20.0% (volume/volume), agar 6.0% (mass/volume), pH value is
On 6.0 MS culture mediums.Intensity of illumination is 1500Lux, daily 14 hours of light application time, and cultivation temperature is in 24 DEG C, 45 days subcultures
Once, sustainable more than 1 year of the callus differentiation capability.795 plants of regeneration can be differentiated from one piece of callus by subculture repeatedly
Seedling.
3. strong seedling culture：Regrowth goes to the natural coconut juices of 1/2MS+20% (volume/volume), and pH value is trained for 6.0 strong sprout
Support on base, intensity of illumination 1500Lux, daily 14 hours of light application time, cultivation temperature is at 24 DEG C.
4. Transplantation of Regenerated Plantlets：Strong sprout plant is grown to after 3~4 centimetres, and the tissue culture bottle of strong sprout plant is taken in greenhouse and beaten
Open, strong sprout plant is adapted to greenhouse after 7 days, strong sprout plant is taken out from tissue culture bottle, culture medium is cleaned up, transplant
Into 100% red beautiful native cultivation matrix, red beautiful soil needs to sterilize 30~60 minutes by 121 DEG C.Pour once within 7 days or so it is permeable just
It is viable.
Using jade dew leafcutting as explant, collocation and the environment conditioning in the external world is cooperateed with to arrange by rational culture medium
Apply, dedifferentiation frequency is up to 43%, and inducing clumping bud rate is up to 84.1% referring now to prior art culture and improvement more than 15%, blade
The callus that explant is produced, produces Multiple Buds, Multiple Buds can differentiate 800 plants or so of regeneration after subculture after differentiation
Seedling, the survival rate of regrowth is transplanted survival rate 100% under control environment, carried referring now to prior art up to 95%
It is high by more than 20%.The present invention can be such that jade is exposed in the relatively short time in the case where not influenceing maternal plant normal development and breeding
Large-scale breeding, and draw materials and be not subject to seasonal restrictions, the regeneration period is short, and callus induction rate is high, and differentiation rate is high, plants
Growth conditions are good after strain is transplanted.It is highly suitable for the quick breeding of beautiful dew Rare Kinds, the cost pole of average individual plant regrowth
It is low, it is especially suitable for factorial praluction.
Claims (6)
1. the beautiful dew rapid propagation in vitro method by explant of blade, it is characterised in that comprise the following steps：
1) explant inoculation and callus induction：The young leaflet tablet of beautiful dew plant is taken, is put into sterile super-clean bench, with 75%
(volume/volume) alcohol-pickled 30~60s, after aseptic water washing 2~3 times, pours into rapidly 1.5~3% (mass/volumes) secondary
8~10min is soaked in sodium chlorate solution, incline liquor natrii hypochloritis, then with aseptic water washing 4~5 times, the jade handled well is revealed
Blade, it is crosscutting into 1cm or so segments；It is seeded on MS solid mediums, can induce when yellow green granular callus is grown point
Change；
2) induction of Multiple Buds：Callus is transferred to inductive differentiation medium；Intensity of illumination is 1500~2000Lux, during illumination
Between daily 12~14 hours, cultivation temperature at 24 DEG C~28 DEG C, 40~50 days subcultures once, the callus differentiation capability sustainable one
More than year；800 plants or so of regrowth can be differentiated from one piece of callus by subculture repeatedly；
3) strong seedling culture：Regrowth goes to the natural coconut juices of 1/2MS+20% (volume/volume), and pH value is trained for 5.6~6.0 strong sprout
Support on base, 1500~2000Lux of intensity of illumination, daily 12~14 hours of light application time, cultivation temperature is at 24 DEG C~28 DEG C；
4) Transplantation of Regenerated Plantlets.
2. rapid propagation in vitro method as claimed in claim 1, it is characterised in that：1) the explant inoculation and callus induction
Middle MS solid mediums include 0.5mg/L containing 6-BA, NAA 0.1mg/L, KT 1mg/L, sucrose 3.0% (mass/volume), fine jade
Fat 6.0% (mass/volume), pH value is 5.6~6.0.
3. rapid propagation in vitro method as claimed in claim 1, it is characterised in that：2) induction differentiation training in the induction of the Multiple Buds
Foster base is included：：MS+KT0.5mg/L+6-BA (0.1~0.2mg/L)+NAA (0~0.1mg/L)+IAA (0.1~0.5mg/L)+
20.0% natural coconut juice (volume/volume), agar 6.0% (mass/volume), pH value is on 5.6~6.0 MS culture medium.
4. rapid propagation in vitro method as claimed in claim 1, it is characterised in that：3) strong seedling culture：Regrowth goes to 1/2MS
+ 20% natural coconut juice (volume/volume), pH value is on 5.6~6.0 strong seedling culture base.
5. rapid propagation in vitro method as claimed in claim 1, it is characterised in that：The intensity of illumination is 1500~2000Lux, light
Daily 12~14 hours according to the time, cultivation temperature is at 24 DEG C~28 DEG C, and 50 days subcultures are once.
6. rapid propagation in vitro method as claimed in claim 1, it is characterised in that：4) Transplantation of Regenerated Plantlets：：Strong sprout plant grows to 3
After~4 centimetres, the tissue culture bottle of strong sprout plant is taken in greenhouse and opened, strong sprout plant is adapted to greenhouse after 5~7 days, will
Strong sprout plant is taken out from tissue culture bottle, and culture medium is cleaned up, and is transplanted into 100% vermiculite or red beautiful native cultivation matrix,
Cultivation matrix needs to sterilize 30~60 minutes by 121 DEG C；Pour once within 7~15 days or so permeable just viable.
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| CN201710429395.0A CN107155890B (en) | 2017-06-08 | 2017-06-08 | Jade dew in-vitro rapid propagation method taking leaves as explants |
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| CN201710429395.0A CN107155890B (en) | 2017-06-08 | 2017-06-08 | Jade dew in-vitro rapid propagation method taking leaves as explants |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108401911A (en) * | 2018-06-13 | 2018-08-17 | 内蒙古自治区生物技术研究院 | Jade dew tissue culture medium (TCM) and preparation method thereof |
| CN113841616A (en) * | 2021-11-15 | 2021-12-28 | 南京林业大学 | Gynura bicolor callus high-frequency differentiation culture medium and Gynura bicolor callus culture method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104756871A (en) * | 2015-04-22 | 2015-07-08 | 南京晓庄学院 | Tissue culture method of haworthia retusa |
| CN104782486A (en) * | 2015-04-23 | 2015-07-22 | 陕西师范大学 | Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer |
| CN105638463A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Tissue-culture rapid propagation method for succulents |
| CN105746352A (en) * | 2016-03-10 | 2016-07-13 | 浙江大学 | Tissue culture method of succulent Haworthia emelyae v.comptoniana 'KYODAI AKASEN' HO1 |
| CN106577294A (en) * | 2016-12-24 | 2017-04-26 | 河南中医药大学 | In-vitro rapid propagation method for haworthia cooperi v. truncata |
-
2017
- 2017-06-08 CN CN201710429395.0A patent/CN107155890B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104756871A (en) * | 2015-04-22 | 2015-07-08 | 南京晓庄学院 | Tissue culture method of haworthia retusa |
| CN104782486A (en) * | 2015-04-23 | 2015-07-22 | 陕西师范大学 | Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer |
| CN105638463A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Tissue-culture rapid propagation method for succulents |
| CN105746352A (en) * | 2016-03-10 | 2016-07-13 | 浙江大学 | Tissue culture method of succulent Haworthia emelyae v.comptoniana 'KYODAI AKASEN' HO1 |
| CN106577294A (en) * | 2016-12-24 | 2017-04-26 | 河南中医药大学 | In-vitro rapid propagation method for haworthia cooperi v. truncata |
Non-Patent Citations (5)
| Title |
|---|
| SUZANNE M. DETHIER ROGERS: "Optimization of plant regeneration and rooting from leaf explants of five rare Haworthia", 《SCIENTIA HORTICULTURAE》 * |
| 何佳越等: "帝玉露的离体培养及快速繁殖技术研究", 《安徽农业科学》 * |
| 杨继富等: "《农村安全供水技术研究》", 31 July 2015, 中国水利水电出版社 * |
| 郝志华等: "常见多肉植物繁育技术的研究进展", 《广东蚕业》 * |
| 郭生虎等: "百合科十二卷属玉露的组培快繁关键技术研究", 《中国农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108401911A (en) * | 2018-06-13 | 2018-08-17 | 内蒙古自治区生物技术研究院 | Jade dew tissue culture medium (TCM) and preparation method thereof |
| CN113841616A (en) * | 2021-11-15 | 2021-12-28 | 南京林业大学 | Gynura bicolor callus high-frequency differentiation culture medium and Gynura bicolor callus culture method |
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| CN107155890B (en) | 2021-02-09 |
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