CN104782486A - Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer - Google Patents
Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer Download PDFInfo
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Abstract
The invention discloses a tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer. The method comprises the following steps: selecting Haworthia cooperivar. pilfera M. B. Bayer leaves in a good growing state as explants, after disinfecting the explants, inoculating the disinfected explants to clustered shoot inducting medium for inducing clustered shoots, then placing the clustered shoots into a rooting medium for taking roots, or after subculturing of the clustered shoots, placing the clustered shoots into the rooting medium for taking roots, and then carrying out seedling adaptation and plant transplanting on intact plantlets after root growing. According to the method, Haworthia cooperivar. pilfera M. B. Bayer seedlings can be obtained by tissue culturing to Haworthia cooperivar. pilfera M. B. Bayer leaves, the material used in the method is easy to obtain, the disinfecting is convenient, the survival rate is high, the production period is short, the propagation coefficient and the propagation speed of the Haworthia cooperivar. pilfera M. B. Bayer are improved, the strain advantages of the Haworthia cooperivar. pilfera M. B. Bayer can be well kept, a great amount of excellent Haworthia cooperivar. pilfera M. B. Bayer seedlings suitable for transplanting can be propagated quickly, the production demands are met, and the economic benefits of planters are improved.
Description
Technical field
The present invention relates to a kind of Plant Tissue Breeding method for quickly breeding.
Background technology
Beautiful dew (Haworthia cooperivar.pilfera M.B.Bayer) belongs to the soft leaf system kind in succulent for american agave order Eremurus chinensis section (also claiming Asphodelaceae, Aloeaceae or A Fu flower section) 12 volumes; originate in South Africa, this life circle, many ground can be cultivated.Plant is just Dan Sheng, and later gradually in all living creatures's shape, fleshy leaf is arranged in rosette-stape, and garden, both sides is convex; Blade tip is transparent or translucent, is called " window ", and there is longitudinal dark strokes on surface, and there is tiny palpus on top, and leaf look dark green; Loose raceme, little floral white, has green vertical stripe.Beautiful dew is small and exquisite, blade is glittering and translucent, forms as jade carving, peculiar and beautiful, as lived handicraft, very lovely, intersperse the places such as desk, desk, windowsill, Fresh and elegant by little potted plant kind, famous and precious indoor appreciation flowers, be one of small-sized succulent kind that popularity is more prosperous in recent years, be subject to pursuing of succulent fan, there is good market prospects and economic worth.
The common propagation method of beautiful dew has cuttage, plant division, leaf are inserted and the several method such as sowing.But for jade dew, these method reproduction speeds are comparatively slow, and efficiency is lower, and planting seed is utilized also to be unfavorable for preserving rare famous and precious strain.And only there is an example in existing Yu Lu tissue culturing system, be utilize beautiful dew scape or scape ovary as explant induction Multiple Buds, the acquisition of material is subject to the restriction of growth cycle, is unfavorable for putting into production.
Summary of the invention
Technical problem to be solved by this invention is providing a kind of quick breeding method for tissue culture of jade dew.
Solve the problems of the technologies described above adopted technical scheme to be made up of following step:
1, the selection of explant and sterilization
With jade dew blade for explant, jade clean for surface clean dew blade is first soaked 1 minute with the mercury chloride that mass fraction is 0.1% on superclean bench, 5 minutes are soaked again with the aqueous sodium hypochlorite solution that mass fraction is 5%, each immersion is complete rear all with sterile water washing, obtains aseptic explant.
2, the induction of Multiple Buds
Aseptic explant is cut into the section of 0.3 ~ 0.6cm, being inoculated in induced bundle sprouts in medium, 25 ± 2 DEG C, intensity of illumination 1000 ~ 1200lux, light application time be 12 ~ 16 hours/day, cultivate 14 ~ 20 days under the environment of relative moisture 50% ~ 60%, obtain Multiple Buds, wherein induced bundle medium of sprouting is for minimal medium with MS medium, adding 0.8 ~ 1.2mg 6-benzyladenine, 0.8 ~ 1.2mg 6-glycosyl aminopurine, 25 ~ 30g sucrose, 5.0 ~ 6.0g agar in often liter of minimal medium, is 5.5 ~ 6.0 by NaOH adjust ph.
3, Multiple Buds is taken root
Be directly to proceed in root media culture of rootage 3 ~ 6 weeks after a strain is separated by Multiple Buds by 3 ~ 4 buds, obtain the whole plant of band root; Or be first to proceed in subculture medium squamous subculture 4 ~ 8 weeks after a strain is separated by Multiple Buds by 3 ~ 4 buds, then by the Multiple Buds after squamous subculture by 8 ~ 10 buds be one strain be separated, to proceed in root media culture of rootage again 3 ~ 6 weeks, obtain the whole plant being with root.
Above-mentioned culture of rootage and squamous subculture condition are: temperature 25 ± 2 DEG C, intensity of illumination 1000 ~ 1200lux, light application time are 12 ~ 16 hours/day, relative moisture 50% ~ 60%; Described root media is with 1/2MS medium for minimal medium, adds 0.08 ~ 0.12mg indolebutyric acid, 25 ~ 30g sucrose, 5.0 ~ 6.0g agar in often liter of minimal medium, is 5.5 ~ 6.0 by NaOH adjust ph; Described subculture medium is with MS medium for minimal medium, adds 0.15 ~ 0.25mg 6-benzyladenine, 1 ~ 1.5g active carbon, 25 ~ 30g sucrose, 5.0 ~ 6.0g agar in often liter of minimal medium, is 5.5 ~ 6.0 by NaOH adjust ph.
4, hardening and transplanting
The whole plant of band root is opened tissue culture bottle bottle cap in 25 ± 2 DEG C of incubators, add running water, hardening was taken out after 1 ~ 2 day, the root media that root remains is rinsed well, In Shade air-dry, transplant in sand soil, shade, temperature be 15 ~ 25 DEG C, relative moisture be 50% ~ 70% time growth.
In above-mentioned steps 2, preferred induced bundle medium of sprouting is with MS medium for minimal medium, and adding 1.0mg 6-benzyladenine, 1.0mg 6-glycosyl aminopurine, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.
In above-mentioned steps 3, preferred root media is with 1/2MS medium for minimal medium, adds 0.1mg indolebutyric acid, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph; Preferred subculture medium is with MS medium for minimal medium, adds 0.2mg 6-benzyladenine, 1g active carbon, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.
The present invention utilizes beautiful dew blade to be explant, by cultivating to jade dew leaf tissue the plantlet in vitro obtaining beautiful dew, the method material is easy to get, and sterilization is convenient, and survival rate is high, with short production cycle, improve reproduction coefficient and the reproduction speed of beautiful dew, and the strain advantage of beautiful dew can be kept preferably, a large amount of excellent jade dew seedling being applicable to transplanting can be gone out by Fast-propagation, meet Production requirement, improve the economic benefit of grower.
Accompanying drawing explanation
Fig. 1 is the photo of the Multiple Buds that embodiment 1 obtains.
Fig. 2 is the photo of the whole plant of the band root that embodiment 1 obtains.
Fig. 3 is the photo of the Multiple Buds obtained after embodiment 2 squamous subculture.
Fig. 4 is the photo of the whole plant of the band root obtained after embodiment 2 culture of rootage.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in more detail, but protection scope of the present invention is not limited only to these embodiments.
Embodiment 1
1, the selection of explant and sterilization
Choose beautiful dew blade, wash beautiful dew blade surface earth and use running water again 5 hours, use filter paper suck dry moisture.Superclean bench first soaks beautiful dew blade 1 minute with the mercury chloride that mass fraction is 0.1%, then with sterile water washing 3 ~ 5 times, each 3 ~ 5 minutes; Again jade is revealed blade proceed to mass fraction be in the aqueous sodium hypochlorite solution of 5% soak 5 minutes, then with sterile water washing 4 ~ 6 times, each 5 ~ 7 minutes, obtain aseptic explant.
2, the induction of Multiple Buds
Aseptic explant is cut into the section of about 0.5cm, the induced bundle be inoculated in tissue culture bottle is sprouted in medium, every bottle graft kind 4 ~ 6,25 ± 2 DEG C, intensity of illumination 1000lux, light application time be 14 hours/day, cultivate 14 days under the environment of relative moisture 50% ~ 60%, obtains Multiple Buds.Wherein induced bundle medium of sprouting is with MS medium for minimal medium, and adding 1.0mg 6-benzyladenine, 1.0mg 6-glycosyl aminopurine, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.As seen from Figure 1, through 14 days Fiber differentiation, beautiful dew blade induced synthesis Multiple Buds, and all blades can differentiate the bud of more than 10.
3, Multiple Buds is taken root
Be in the root media proceeding in tissue culture bottle after a strain is separated by 3 ~ 4 buds by Multiple Buds, every bottle of 3 ~ 4 strains, be 14 hours/day in temperature 25 ± 2 DEG C, intensity of illumination 1000lux, light application time, cultivate 3 weeks under the environment of relative moisture 50% ~ 60%, obtain the whole plant of band root.Wherein root media is with 1/2MS medium for minimal medium, adds 0.10mg indolebutyric acid, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.As seen from Figure 2, in root media, culture of rootage is after 3 weeks, and beautiful dew unrooted shoot has grown the new root of white, and physically well develops, and rooting rate reaches more than 75%.
4, hardening and transplanting
The whole plant of band root is opened tissue culture bottle bottle cap in 25 ± 2 DEG C of incubators, add running water, hardening was taken out after 1 day, the root media that root remains is rinsed well, In Shade air-dry 1 day, transplant in sand soil, shade, temperature be 20 DEG C, relative moisture be 50% ~ 60% time growth.
Embodiment 2
1, the selection of explant and sterilization
Choose beautiful dew blade, wash beautiful dew blade surface earth and use running water again 5 hours, use filter paper suck dry moisture.Superclean bench first soaks beautiful dew blade 1 minute with the mercury chloride that mass fraction is 0.1%, then with sterile water washing 3 ~ 5 times, each 3 ~ 5 minutes; Again jade is revealed blade proceed to mass fraction be in the aqueous sodium hypochlorite solution of 5% soak 5 minutes, then with sterile water washing 4 ~ 6 times, each 5 ~ 7 minutes, obtain aseptic explant.
2, the induction of Multiple Buds
Aseptic explant is cut into the section of about 0.5cm, the induced bundle be inoculated in tissue culture bottle is sprouted in medium, every bottle graft kind 4 ~ 6,25 ± 2 DEG C, intensity of illumination 1000lux, light application time be 14 hours/day, cultivate 14 days under the environment of relative moisture 50% ~ 60%, obtains Multiple Buds.Wherein induced bundle medium of sprouting is with MS medium for minimal medium, and adding 1.0mg 6-benzyladenine, 1.0mg 6-glycosyl aminopurine, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.
3, Multiple Buds is taken root
Be in the subculture medium proceeding in tissue culture bottle after a strain is separated by 3 ~ 4 buds by Multiple Buds, every bottle of 3 ~ 4 strains, temperature 25 ± 2 DEG C, intensity of illumination 1000lux, light application time is 14 hours/day, cultivate 4 weeks under the environment of relative moisture 50%-60%, then by the Multiple Buds after squamous subculture by 8 ~ 10 buds be one strain be separated, proceed in the root media in tissue culture bottle again, every bottle of 3 ~ 4 strains, temperature 25 ± 2 DEG C, intensity of illumination 1000lux, light application time is 14 hours/day, cultivate 3 weeks under the environment of relative moisture 50% ~ 60%, obtain the whole plant being with root.Wherein root media is with 1/2MS medium for minimal medium, adds 0.10mg indolebutyric acid, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.As seen from Figure 3, formed after squamous subculture 4 weeks grow fine, healthy and strong Multiple Buds bunch; As seen from Figure 4, take root good, sturdy by the seedling carrying out culture of rootage after squamous subculture again.
4, hardening and transplanting
The whole plant of band root is opened tissue culture bottle bottle cap in 25 ± 2 DEG C of incubators, add running water, hardening was taken out after 1 day, the root media that root remains is rinsed well, In Shade air-dry 1 day, transplant in sand soil, shade, temperature be 20 DEG C, relative moisture be 50% ~ 60% time growth.
Claims (4)
1. a quick breeding method for tissue culture for succulent jade dew, is characterized in that it comprises the steps:
(1) selection of explant and sterilization
With jade dew blade for explant, jade clean for surface clean dew blade is first soaked 1 minute with the mercury chloride that mass fraction is 0.1% on superclean bench, 5 minutes are soaked again with the aqueous sodium hypochlorite solution that mass fraction is 5%, each immersion is complete rear all with sterile water washing, obtains aseptic explant;
(2) induction of Multiple Buds
Aseptic explant is cut into the section of 0.3 ~ 0.6cm, being inoculated in induced bundle sprouts in medium, 25 ± 2 DEG C, intensity of illumination 1000 ~ 1200lux, light application time be 12 ~ 16 hours/day, cultivate 14 ~ 20 days under the environment of relative moisture 50% ~ 60%, obtain Multiple Buds, wherein induced bundle medium of sprouting is for minimal medium with MS medium, adding 0.8 ~ 1.2mg 6-benzyladenine, 0.8 ~ 1.2mg 6-glycosyl aminopurine, 25 ~ 30g sucrose, 5.0 ~ 6.0g agar in often liter of minimal medium, is 5.5 ~ 6.0 by NaOH adjust ph;
(3) Multiple Buds is taken root
Be directly to proceed in root media culture of rootage 3 ~ 6 weeks after a strain is separated by Multiple Buds by 3 ~ 4 buds, obtain the whole plant of band root; Or be first to proceed in subculture medium squamous subculture 4 ~ 8 weeks after a strain is separated by Multiple Buds by 3 ~ 4 buds, then by the Multiple Buds after squamous subculture by 8 ~ 10 buds be one strain be separated, to proceed in root media culture of rootage again 3 ~ 6 weeks, obtain the whole plant being with root;
Above-mentioned culture of rootage and squamous subculture condition are: temperature 25 ± 2 DEG C, intensity of illumination 1000 ~ 1200lux, light application time are 12 ~ 16 hours/day, relative moisture 50% ~ 60%; Described root media is with 1/2MS medium for minimal medium, adds 0.08 ~ 0.12mg indolebutyric acid, 25 ~ 30g sucrose, 5.0 ~ 6.0g agar in often liter of minimal medium, is 5.5 ~ 6.0 by NaOH adjust ph; Described subculture medium is with MS medium for minimal medium, adds 0.15 ~ 0.25mg 6-benzyladenine, 1 ~ 1.5g active carbon, 25 ~ 30g sucrose, 5.0 ~ 6.0g agar in often liter of minimal medium, is 5.5 ~ 6.0 by NaOH adjust ph;
(4) hardening and transplanting
The whole plant of band root is opened tissue culture bottle bottle cap in 25 ± 2 DEG C of incubators, add running water, hardening was taken out after 1 ~ 2 day, the root media that root remains is rinsed well, In Shade air-dry, transplant in sand soil, shade, temperature be 15 ~ 25 DEG C, relative moisture be 50% ~ 70% time growth.
2. the quick breeding method for tissue culture of succulent jade dew according to claim 1, it is characterized in that: in described step (2), induced bundle medium of sprouting is for minimal medium with MS medium, adding 1.0mg 6-benzyladenine, 1.0mg 6-glycosyl aminopurine, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.
3. the quick breeding method for tissue culture of succulent jade dew according to claim 1, it is characterized in that: in described step (3), root media is for minimal medium with 1/2MS medium, adding 0.1mg indolebutyric acid, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.
4. the quick breeding method for tissue culture of succulent jade dew according to claim 1, it is characterized in that: in described step (3), subculture medium is for minimal medium with MS medium, adding 0.2mg 6-benzyladenine, 1g active carbon, 25g sucrose, 5.0g agar in often liter of minimal medium, is 5.8 by NaOH adjust ph.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010147A (en) * | 2015-08-14 | 2015-11-04 | 泓柯（天津）农业科技有限公司 | Special culture medium for improving tissue culture propagation speed of haworthia succulent plants and tissue culture method |
| CN105230487A (en) * | 2015-10-13 | 2016-01-13 | 宁波市鄞州万众生态果园专业合作社 | Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula |
| CN105638463A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Tissue-culture rapid propagation method for succulents |
| CN105766635A (en) * | 2016-03-14 | 2016-07-20 | 龙岩市禾康生物科技有限公司 | Method for tissue culture and rapid propagation of succulent plants |
| CN106258902A (en) * | 2016-08-31 | 2017-01-04 | 昆明理工大学 | The efficient leaf of a kind of succulent inserts propagation method |
| CN106258397A (en) * | 2016-08-12 | 2017-01-04 | 广西鑫雅皇庭园林工程有限责任公司 | A kind of implantation methods of Bryophyllum succulent |
| CN106857256A (en) * | 2017-02-21 | 2017-06-20 | 淮北师范大学 | The method that beautiful dew breeding potential is improved based on callus induction Regeneration Ways |
| CN107155890A (en) * | 2017-06-08 | 2017-09-15 | 北京农学院 | Beautiful dew rapid propagation in vitro method by explant of blade |
| CN107278849A (en) * | 2017-06-29 | 2017-10-24 | 安徽强农牧业有限公司 | A kind of preparation method for attached succulent culture medium particle of breathing freely |
| CN108401911A (en) * | 2018-06-13 | 2018-08-17 | 内蒙古自治区生物技术研究院 | Jade dew tissue culture medium (TCM) and preparation method thereof |
| CN108719065A (en) * | 2018-05-21 | 2018-11-02 | 句容市茂润苗木有限公司 | A kind of succulent rapid propagation method |
| CN110089431A (en) * | 2019-05-06 | 2019-08-06 | 绵阳师范学院 | The quick breeding by group culture method of jade dew |
| CN110447538A (en) * | 2019-08-30 | 2019-11-15 | 江苏省中国科学院植物研究所 | It is a kind of that rachis is revealed as the method for tissue culture of explant using Pan Shi ice lantern jade |
| CN115399243A (en) * | 2022-08-26 | 2022-11-29 | 内蒙古科技大学 | Tissue culture rapid propagation method for inducing regeneration of adventitious buds by taking rubiaceae yulu leaves as explants |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499090A (en) * | 2011-11-07 | 2012-06-20 | 上海旭东园艺有限公司 | Method for isolated culture of Haworthia succulent plants |
| CN103141387A (en) * | 2013-03-08 | 2013-06-12 | 浙江省农业科学院 | Method for cultivating haworthia maughanii tissue |
| CN105230487A (en) * | 2015-10-13 | 2016-01-13 | 宁波市鄞州万众生态果园专业合作社 | Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula |
-
2015
- 2015-04-23 CN CN201510197424.6A patent/CN104782486B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499090A (en) * | 2011-11-07 | 2012-06-20 | 上海旭东园艺有限公司 | Method for isolated culture of Haworthia succulent plants |
| CN103141387A (en) * | 2013-03-08 | 2013-06-12 | 浙江省农业科学院 | Method for cultivating haworthia maughanii tissue |
| CN105230487A (en) * | 2015-10-13 | 2016-01-13 | 宁波市鄞州万众生态果园专业合作社 | Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula |
Non-Patent Citations (2)
| Title |
|---|
| 陈红刚等: "玉露的组织培养与快速繁殖", 《北方园艺》 * |
| 高越等: "毛玉露的组织培养与快速繁殖", 《生物学通报》 * |
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| CN105010147A (en) * | 2015-08-14 | 2015-11-04 | 泓柯（天津）农业科技有限公司 | Special culture medium for improving tissue culture propagation speed of haworthia succulent plants and tissue culture method |
| CN105230487A (en) * | 2015-10-13 | 2016-01-13 | 宁波市鄞州万众生态果园专业合作社 | Haworthia cooperivar. pilfera M. B. Bayer OB-1 tissue culture formula |
| CN105638463A (en) * | 2015-12-30 | 2016-06-08 | 四川禾木本业农林科技有限公司 | Tissue-culture rapid propagation method for succulents |
| CN105766635B (en) * | 2016-03-14 | 2017-12-22 | 龙岩市禾康生物科技有限公司 | A kind of method of succulent tissue-culturing rapid propagation |
| CN105766635A (en) * | 2016-03-14 | 2016-07-20 | 龙岩市禾康生物科技有限公司 | Method for tissue culture and rapid propagation of succulent plants |
| CN106258397A (en) * | 2016-08-12 | 2017-01-04 | 广西鑫雅皇庭园林工程有限责任公司 | A kind of implantation methods of Bryophyllum succulent |
| CN106258902A (en) * | 2016-08-31 | 2017-01-04 | 昆明理工大学 | The efficient leaf of a kind of succulent inserts propagation method |
| CN106857256A (en) * | 2017-02-21 | 2017-06-20 | 淮北师范大学 | The method that beautiful dew breeding potential is improved based on callus induction Regeneration Ways |
| CN107155890A (en) * | 2017-06-08 | 2017-09-15 | 北京农学院 | Beautiful dew rapid propagation in vitro method by explant of blade |
| CN107278849A (en) * | 2017-06-29 | 2017-10-24 | 安徽强农牧业有限公司 | A kind of preparation method for attached succulent culture medium particle of breathing freely |
| CN108719065A (en) * | 2018-05-21 | 2018-11-02 | 句容市茂润苗木有限公司 | A kind of succulent rapid propagation method |
| CN108401911A (en) * | 2018-06-13 | 2018-08-17 | 内蒙古自治区生物技术研究院 | Jade dew tissue culture medium (TCM) and preparation method thereof |
| CN110089431A (en) * | 2019-05-06 | 2019-08-06 | 绵阳师范学院 | The quick breeding by group culture method of jade dew |
| CN110447538A (en) * | 2019-08-30 | 2019-11-15 | 江苏省中国科学院植物研究所 | It is a kind of that rachis is revealed as the method for tissue culture of explant using Pan Shi ice lantern jade |
| CN115399243A (en) * | 2022-08-26 | 2022-11-29 | 内蒙古科技大学 | Tissue culture rapid propagation method for inducing regeneration of adventitious buds by taking rubiaceae yulu leaves as explants |
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