KR100819173B1 - Cosmetic compostion comprising vitamin c - Google Patents

Abstract

A cosmetic composition comprising vitamin C is provided to preserve the vitamin C for 3 years or more by keeping the vitamin C in a solid form from a liquid if there is no artificial temperature increase, strong transmission of light and supply of oxygen and also maintain the vitamin C stable for at least 6 months when in use by being mixed with the liquid. In a cosmetic composition including a solid containing vitamin C and a liquid phase separately to mix them right before use, the solid further comprises at least one antioxidizing agent selected from the group consisting of kojic acid, alpha-lipoic acid, proanthocyanidin, retinol, a retinol derivative, co-enzyme Q10, a cysteine-based antioxidizing agent, selenium, pycnogenol, enzogenol and fingenol and carbonate is dissolved in demineralized water or de-ionized water to decrease dissolved oxygen in the liquid phase. The composition further comprises at least one selected from the group consisting of a moisturizer, a surfactant, a thickener, a pH adjusting agent and a sequestering agent.

Description

Cosmetic composition capable of applying vitamin C stably for a long time {Cosmetic compostion comprising Vitamin C}

The present invention has a composition for inhibiting skin wrinkle formation and whitening effect but inhibiting the oxidation of vitamin C, which is limited in use due to instability, which is easily oxidized by dissolved oxygen in water, so that the bioactive effect is not destroyed. In particular, the present invention relates to a cosmetic composition capable of applying vitamin C stably for a long time by separating, filling and containing a solid and a liquid containing vitamin C so as to be mixed immediately before use.

In the field of cosmetic and skin pharmaceutical preparations, vitamin C has many advantages.

Dermato Surg. 31 (July 2005) pages 814-817, Clin. Exp. Dermatol. As published in Vol. 29, No. 2 (March 2004), pages 168 to 175, and Exp. Dermatol. Vol. 12, No. 3 (June 2003), pages 237 to 244, vitamin C contains lysine in collagen. It acts as a cofactor for the hydroxylation of lysine and proline amino acid residues (Cofactor), which stimulates collagen synthesis to give skin elasticity and reduce skin wrinkles.

J Invest Dermatol. Vol. 126, No. 5 (May 2006) Pages 1185 to 1187, Free Radic Res. 40, No. 2 (February 2006) pp. 213-221, J Invest Dermato. As published in 125, No. 4 (October 2005) pages 826 ~ 832, Vitamin C is a powerful antioxidant that improves the defense of skin tissue by eliminating the free radicals generated in the environment where the skin receives ultraviolet rays. It also supplements vitamin E deficiency, another bio-oxidant that acts as an antioxidant in the skin, and prevents pigmentation of the skin by inhibiting melanin production from pigment cells in epidermal tissue (Int J Dermatol, Vol. 43, No. 8 ( August 2004) pages 604 to 607, Biofactors Volume 21 pages 415 to 418). For this reason, it is an excellent candidate material for cosmetics and skin medicines for the treatment and / or prevention of skin aging in relation to pigmentation and wrinkles.

However, vitamin C has been found to contain light, oxygen, and water (pH) due to its chemical structure (α-ketolactone, which has a double bond between the 2nd and 3rd carbons, and the 2nd and 3rd carbons have a hydroxyl group). And in the presence of a small amount of metal), the electrons are easily lost and oxidized to prevent the physiological activity of vitamin C. Therefore, many efforts have been made to obtain cosmetics that are stable to oxidation in the air, and various prior arts have been reported.

One of them is phosphate (JP7017989, JP8034791, JP98363316, JP98201242, JP10324627, US Pat. No. 3,671,549, DE1805958, US Pat.No. 5,098,898,), Sulphate or alkyl compounds (2,3-di-O-acyl L-ascorbic acids (JP63104971, DE3613590) US-A-5,087,446, JP-A-69 / 115558 and JP-A-83 / And esterification or etherification so that the electrons are not lost easily.

In US Pat. No. 2,400,171, Ruskin uses a technique to chloride vitamin C with calcium or zinc ions to maintain a stable aqueous solution within a pH range of 7 to 7.3. In US Pat. No. 2,442,461, Karrer uses a calcium aqueous solution of vitamin C. A technique for stabilizing by the addition of Alphahatic thiocarboxylic acid and keeping it in the range of pH 5.2 to 5.6, Opplt in US Pat. No. 2,585,580, vitamin C is thio-sugar sugars) and a technique for stabilization in the range of pH 4.0 to 6.5, Sherman in US Pat. No. 4,367,157 invented the addition of monothioglycerol to obtain a stable aqueous solution in the range of pH 4-7. .

Another method is to separate vitamin C from oxidative factors so that vitamin C does not oxidize. In US Pat. No. 4,372,874, Modrovich stabilized through the invention of adding a desiccant capable of binding water to vitamin C solution to lower the amount of water remaining below 0.5%. In US Pat. No. 5,140,043, Darr et al. Vitamin C was stabilized by combining C 1% with a transport consisting of water, alkylene glycol, and hydroxyalkylcellulose.

In U.S. Pat. No. 5, 296, 249, Todd invented stabilizing micro-sized vitamin C particles in a dispersion medium that does not dissolve the vitamin C particles. U.S.Pat. In No. 5,308, 621 Taylor et al. Invented successfully dispersing small particulate vitamin C in drug transporters such as glycol or petroleum jelly to facilitate transdermal absorption.

U.S. Pat. No. 5,587, 149 invented a technique for a PEG in oil emulsion product of vitamin C.

Another solution is to use vitamin C in powder form to dissolve it in water just before use. Korean Patent 0178377 has invented a skin pathological composition that is used to store vitamin C in a solid state and then mixed with a hydrophilic gelling agent resistant to acid immediately before use. Document FR-A-2,645,740 also discloses a composition containing an oil phase, an aqueous phase and a solid phase immediately before use.

The solid phase of the document FR-A-2,645,740 contains at least one active agent (particularly vitamin A) and emulsifiers and the other two contain liquid phases, which are spatially separated and then mixed with three phases upon use. The preservation time of the composition is long before, but after mixing the composition has a long settling time and the resulting mixture is a non-homogeneous crude. In addition, there is a disadvantage that the stability of the emulsion is not secured to contain the active ingredient having an acid.

In addition, Korean Patent 0178377 invents a composition in which vitamin C is stored separately in a solid state and mixed with an acid-resistant hydrophilic carrier just before use. When it is in solid state before use, vitamin C is more stable than dissolved in water. It can be preserved, but it is difficult to completely block the water molecules or oxygen molecules, so the vitamin C is also hydrated and discolored through oxidation after a long time.

In addition, if it is mixed with the aqueous phase when used on the skin and is not used within a short time, the vitamin C dissolved in the aqueous solution will start to break down, and the content of vitamin C will decrease, and at the same time, it will be discolored and discolored, thereby damaging the value of the product There is this.

The present invention is a way to overcome these shortcomings, vitamin C is maintained in a solid form, separated from the liquid and circulated separately and can be preserved for a long time without separate artificial temperature rise, strong light transmission, oxygen supply, etc. It is to provide a cosmetic composition in which the vitamin C remains stable for a long time after the start of mixing with the liquid, that is, even after the use of the product.

The present invention to achieve the above technical problem

In the cosmetic composition is separated and contained so that the solid and liquid phase containing vitamin C is mixed immediately before use,

The solid is kojic acid (Kojic acid), alpha-lipoic acid (alpha-lipoic acid), proanthocyanidin (Proanthocyanidin), retinol (Retinol), retinol derivatives, coenzyme Q10, cysteine-based antioxidants, selenium, pycnogenol (pycnogenol), enzogenol and fingenol further comprises any one or more antioxidants selected from the group consisting of

The liquid phase is characterized by reducing dissolved oxygen by dissolving carbonate in demineralized to deionized water to provide a cosmetic composition that can be applied to vitamin C stably for a long time.

In the cosmetic composition according to the present invention as described above, the vitamin C is 0.1 to 20% of the total weight of the composition, the antioxidant is 0.1 to 10% of the total weight of the composition, the carbonate is 0.05 to 0.5% of the total weight of the composition It is preferable to contain.

In addition, in the cosmetic composition according to the present invention as described above, it is preferable that the liquid phase further comprises any one or more selected from the group consisting of a moisturizer, a surfactant, a thickener, a pH adjusting agent and a metal ion blocking agent.

As described above, the cosmetic composition of the present invention can be stored for a long period of time more than 3 years if vitamin C is maintained in a solid form and separated from the liquid and circulated separately and there is no separate artificial temperature rise, strong light transmission, oxygen supply, etc. Vitamin C starts to mix with the liquid, that is, for a long time even after the start of use of the product, there is an advantage that the vitamin C remains stable for at least six months.

The present invention relates to a cosmetic composition which is contained in a container in which a solid and a liquid containing vitamin C are spatially separated and then mixed so as to be mixed immediately before use. May be used without limitation as long as it is known in the art.

In the cosmetic composition containing a solid containing a vitamin C and the liquid as described above in a spatially separated container to be mixed immediately before use, in the present invention, the solid is koji acid (Kojic acid), Alpha-lipoic acid, proanthocyanidin, retinol, retinol, retinol derivatives, coenzyme Q10, cysteine-based antioxidants, selenium, pycnogenol, enzogenol And one or more antioxidants selected from the group consisting of fingenol, wherein the liquid phase dissolves the carbonate in demineralized to deionized water to reduce dissolved oxygen to stably apply vitamin C for a long time. It is characterized by that it was possible to.

The cysteine-based sulfate agent refers to cysteine, glutathione, and N-acetyl cysteine having a SH (sulfur hydroxyl) functional group, and the proanthocyanidins are grape seed extract and pine bark. It is an antioxidant component contained in extracts and the like. Pine bark extracts containing proanthocyanidins include pycnogenol from France, enzogenol from New Zealand, and fingenol from Finland.

Among the antioxidants included in the solid, those present in the liquid state, such as retinol and retinol derivatives, may be mixed with other solid components and solidified. Alternatively, the antioxidant may be artificially coated with a polymer such as Agar or Collagen. What solidified may be used.

As described above, the antioxidant contained in the composition of the present invention has a stronger antioxidant power than vitamin C, thereby oxidizing before vitamin C and protecting vitamin C against external influences, and thus the composition according to the present invention. In the stability of vitamin C is enhanced to ensure the retention period of vitamin C of at least six months.

Such a composition according to the present invention is opened by the first pumping action, the part containing the solid part is mixed with the liquid (되고) and can be mixed by shaking by hand, the present invention through various experiments of the present inventors The composition according to the present invention was confirmed that the vitamin C remains stable at room temperature for at least 6 months after mixing. The six-month period is a period in which vitamin C-containing cosmetics are consumed on average after the consumer starts using them, and thus the cosmetic composition according to the present invention can be applied to the skin without destroying the active ingredient throughout the use time.

In addition, the cosmetic composition according to the present invention, unlike the products of the prior art in which the function of penetrating the skin using a modified derivative for stabilization of the active ingredient to be converted to the active form by enzymes in the skin, the active ingredients are immediately It is much more effective because it is dissolved in the liquid and applied to the skin.

In the composition according to the present invention as described above, the vitamin C is 0.1 to 20% of the total weight of the composition, the antioxidant is 0.1 to 10% of the total weight of the composition, the carbonate contains 0.05 to 0.5% of the total weight of the composition It is desirable to be.

The carbonate is added to remove dissolved oxygen in the liquid phase. When carbonate is added to the liquid phase, carbon dioxide is formed and oxygen is replaced with carbon dioxide having better solubility than oxygen, thereby reducing dissolved oxygen in the liquid phase. Examples of carbonates usable in the compositions according to the invention include sodium bicarbonate.

In addition, in the cosmetic composition according to the present invention as described above, the liquid phase may further include any one or more selected from the group consisting of a moisturizer, a surfactant, a thickener, a pH adjusting agent, a metal ion blocking agent and a water-soluble active ingredient. I can explain them.

The thickeners used in the liquid portion of the present invention are acid probes, carbomers, guar gums, carrageenan gums, cellulose hydroxy alkyl celluloses, sodium carboxy celluloses, polyacrylate based polymers, polyurethane based polymers, polyacrylamide based polymers, gelatin As long as it is known in the art to which the present invention pertains, such as can be used without particular limitation.

In addition, one or more pH adjusters may be added to the liquid portion to prevent the solids from mixing with the liquid portion to form a strongly acidic composition below pH 3.0. For example, sodium lactate, sodium citrate, sodium acetate buffer may be used. Can be. The amount of buffer used is determined by the amount of acidic active ingredient used and the final pH. The final pH is advantageously 3.0 to 6.0, more preferably 3.5 to 5.5.

In the present invention, the moisturizing agent used in the liquid part is glycerin, glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, etc.), pyrrolidone-sodium carboxylate, trehalose, lactic acid, lactate, Examples of amino acids such as arginine and glutamine and polymeric moisturizers include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, sodium hyaluronate, chondroitin sulfate or salts thereof, and gamma polyglutamic acid.

Moreover, surfactant used for this invention contains an emulsion type surfactant or a solubilization type surfactant. Examples of the emulsifying surfactant include polysorbate, sorbitan fatty acid, fatty acid ether, and lecithin. Examples of the solubilizing surfactant include ethoxylated alkyl ether and P.J. hardened castor oil. Oils usable in the present invention include vegetable, synthetic, mineral, silicon and / or fluorinated oils and the like.

In addition, the composition according to the present invention must contain a metal ion sequestrant in the liquid phase to avoid the presence of heavy metals that can catalyze the decomposition and oxidation of the active ingredient in the liquid phase and the liquid phase should be made from demineralized or deionized water. The amount of metal ion sequestrant may generally be selected from 0.01 to 0.2% of the total weight of the composition.

The liquid portion of the present invention may contain other additives apart from those mentioned above, for example, those commonly used in the cosmetic field, such as water-soluble activators, preservatives, colorants, fragrances, etc., which remain stable in liquid form for a long time.

The water-soluble active agent that can be used in the liquid portion is, for example, floral water, hot water plant extract (willow extract acting as an anti-inflammatory and protective agent, soybean extract, safflower extract, chamomile extract, etc.) or an organic solvent and then a cosmetic solvent (for example, Plant extracts (green tea extract, tomato extract, grape extract, coffee extract, etc.) dissolved in 1,3 butylene glycol, ethanol, and propylene glycol), panthenol, hyaluronic acid, etc., and these active agents are generally used up to 5% of the total weight of the composition. do.

In order to preserve the composition of the present invention, the solid and the liquid may be separately packed into two separate containers before mixing to form a single product. In other words, the solids are filled, closed with a stopper, separated, and placed in a larger container containing liquid. Such filling forms are disclosed in documents FR-A-2,476,607, FR-A-2,453,793, FR-A-2,645,740 and Korean Patent 0178377, which may be used.

Given the sensitivity of vitamin C, etc. to light, it is preferable to use opaque and / or amber containers that do not allow light transmission, and the light may be blocked using a cover covering the body of the container.

Powdered vitamin C usable in the present invention is provided in the form of L-ascorbic acid having a purity of 99 to 100%, and the cosmetic composition according to the present invention is used for the whitening effect of wrinkle removal, blemishes, removal or alleviation of senile spots, and ultraviolet irradiation. It can be used to protect skin, regenerate skin tissue and prevent skin aging.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

<Example>

In order to confirm that the oxidation of vitamin C is inhibited by natural antioxidants, the following examples were completed using natural antioxidant components of cysteine, pycnogenol, retinol, and alpha lipophosphate.

Vitamin C 99.0% (Cat # A5960), Cysteine 98.0% ((Cat # C7352) Retinol 95.0% (Cat # R7632) Alpha lipophosphate 99.0% (Cat # T5625) was purchased from Sigma Co., Ltd. Pinus-PA-ES, isolated from the startup company Linux, was used.

Comparative example Example 1 Example 2 Example 3 Example 4 Solid part Vitamin c 10 10 10 10 10 Cysteine 0.5 0.5 0.5 0.5 Proanthocyanidin (Pycnogenol) 0.5 0.5 0.5 Retinol 0.1 0.1 Alpha-Lipoic acid 0.5 Liquid part Polysorbate 60 0.5 0.5 0.5 0.5 0.5 ethanol 2 2 2 2 2 Sodium lactate solution (60%) 2 2 2 2 2 Acid probe 0.1 0.1 0.1 0.1 0.1 Propylene glycol 3 3 3 3 3 Sodium Bicarbonate 0.05 0.05 0.05 0.05 0.05 1,3 butylene glycol 2 2 2 2 2 Metal Ion Blocker 0.1 0.1 0.1 0.1 0.1  Preservative 0.2 0.2 0.2 0.2 0.2  Purified water To100 To 100 To 100 To 100 To 100

Experimental Example: Comparative Experiment of Vitamin C Stabilization of Natural Antioxidant Composition and Simple Vitamin C Composition>

In order to determine that the composite of the complex having an antioxidant function is more useful than the raw material alone, the vitamin C content experiments of Examples 1 to 4 and Comparative Examples were conducted. The experiment was performed in Examples 1-4 and the Comparative Example, which was filled in a container, and the solid portion and the liquid portion were first mixed, and then the mixed contents were sampled every week while stored in an incubator at 30 ° C. The amount of vitamin C was measured.

Vitamin C quantitative method uses high-performance liquid chromatography (HPLC) to precisely weigh the equivalent of 50 mg of ascorbic acid, dissolve it in exactly 50 ml by adding water, and then dissolve the solution in a 0.45 μm syringe filter. Take 1 ml of the filtered solution accurately and make 10 ml with water as the sample solution. Separately, 50 mg of ascorbic acid standard is precisely weighed to make 50 ml of water, and then 1 ml of this solution is precisely prepared to make 10 ml of water as standard solution. Take 10 ㎕ of each sample and standard solution, test by liquid chromatography under the following operating conditions, and determine the peak area AT and As of the sample and standard solutions.

For each sample, the content of the initial value quantified immediately after preparation was expressed as a percentage and compared.

Calculation formula: 1) amount of ascorbic acid (mg / ml) = amount of standard product * area ratio of ascorbic acid in sample / area ratio of standard product

2) Ascorbic acid content ratio (%) = [(amount of ascorbic acid (mg / ml) * amount of diluent solvent * dilution multiple * 100 / weight of sample (mg)) / display content] * 100

The analyzer used Liquid Chromatography (HPLC) (Younglin UV 930 D) and under the following operating conditions.

Column: Waters Atlantis dC18 250 mm * 4.6 mm * 5 μm

Mobile phase: 0.25% phosphate solution (pH 2.0) 100%

Flow rate: 1.0 ml / min

Detector: UV 245 nm

The results are shown in Table 2 by expressing the remaining titer as a percentage that corresponds to the initial content of vitamin C 10%.

Time (week) Comparative example Example 1 Example. 2 Example 3 Example 4 0 101.14% 101.53% 101.88% 101.17% 101.16% One 97.50% 100.07% 100.29% 100.79% 100.34% 2 86.25% 98.23% 99.26% 99.60% 99.79% 3 83.21% 97.75% 97.72% 98.30% 99.30% 4 80.07% 97.58% 97.29% 98.57% 99.26% 5 76.05% 96.74% 97.13% 98.35% 99.30% 6 73.52% 96.25% 97.49% 97.81% 98.60% 7 70.52% 96.58% 96.59% 97.41% 98.53% 8 67.52% 96.10% 96.11% 97.31% 98.85% 9 64.52% 94.29% 95.70% 97.36% 98.52% 12 54.24% 91.51% 93.35% 95.92% 96.91% 16 45.26% 87.76% 91.49% 93.16% 96.03% 20 36.81% 84.17% 88.13% 90.38% 94.77% 24 25.67% 80.39% 85.72% 87.95% 93.82% 28 19.87% 76.53% 81.94% 84.21% 90.18%

As shown in Table 2, it can be seen that Examples 1 to 4, in which the natural antioxidants were combined to constitute a solid component, used a complex having an antioxidant function, which showed higher vitamin C stability than the comparative example used as a single component. As a result, it can be seen that the use of vitamin C containing an antioxidant complex can stabilize the vitamin C to achieve the original effect of vitamin C.

<Test Example 2: Whitening effect test on the human skin>

The whitening function, which is a representative effect on vitamin C, was tested as follows using Examples 1 to 4 and Comparative Examples.

Ten healthy adults (5 females, 5 males) were placed on the upper forearm of the subject, and an opaque tape with 4 holes of 1.5 t diameters was attached and 1.5 times the minimum Erythma Dose of each subject. After irradiating the skin with ultraviolet rays (UVB) to induce skin blackening, the skin was stabilized after one week, and then the composition of Examples 1 to 4 and Comparative Example was applied twice a day (morning and evening) every day and then 8 After weeks, the color value (Lab value) of the skin was measured using a colorimeter (Chrmomameter Minolta CR300). The determination of the effect was based on the 'L' value representing the contrast of the skin. The average Korean skin has an average value of around 60. If the test substance is applied, the L value is gradually increased. The comparison between the two test substances was expressed as ΔL value (final L value-L value before test material application). The larger the ΔL value, the greater the whitening effect. The ΔL values after 8 weeks of treatment are shown in Table 3 below.

Whitening effect (ΔL) Comparative example 2.21 Example 1 2.67 Example 2 2.87 Example 3 2.95 Example 4 3.01

As shown in Table 3, it can be seen that the composition according to the present invention has a greater whitening effect than the comparative example.

Claims (3)

In the cosmetic composition is separated and contained so that the solid and liquid phase containing vitamin C is mixed immediately before use, The solid is kojic acid (Kojic acid), alpha-lipoic acid (alpha-lipoic acid), proanthocyanidin (Proanthocyanidin), retinol (Retinol), retinol derivatives, coenzyme Q10, cysteine-based antioxidants, selenium, pycnogenol (pycnogenol), enzogenol and fingenol further comprises any one or more antioxidants selected from the group consisting of Wherein the liquid is characterized in that dissolved dissolved carbonate in demineralized to deionized water to reduce the dissolved oxygen, cosmetic composition that can be applied for a long time stable vitamin C. The method of claim 1, The vitamin C is 0.1 to 20% of the total weight of the composition, the antioxidant is 0.1 to 10% of the total weight of the composition, the carbonate is characterized in that it contains 0.05 to 0.5% of the total weight of the composition, it is stable to apply vitamin C for a long time Cosmetic composition that can be used. The method according to claim 1 or 2, Cosmetic composition which can be applied to vitamin C for a long time stable, characterized in that the liquid further comprises any one or more selected from the group consisting of a moisturizer, a surfactant, a thickener, a pH adjusting agent and a metal ion blocker.