WO2017195951A1 - Cosmetic and nutritional supplement using aptamer, and method for producing same - Google Patents
Cosmetic and nutritional supplement using aptamer, and method for producing same Download PDFInfo
- Publication number
- WO2017195951A1 WO2017195951A1 PCT/KR2016/011741 KR2016011741W WO2017195951A1 WO 2017195951 A1 WO2017195951 A1 WO 2017195951A1 KR 2016011741 W KR2016011741 W KR 2016011741W WO 2017195951 A1 WO2017195951 A1 WO 2017195951A1
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- WIPO (PCT)
- Prior art keywords
- vitamin
- aptamer
- antioxidant
- extract
- present
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/13—Nucleic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
Definitions
- the present invention relates to cosmetics and nutritional supplements using aptamers and to a method of manufacturing the same, and more particularly, to cosmetics and nutritional supplements using long-term maintenance and antioxidant properties of antioxidants such as reduced vitamin C by aptamers. Is to apply.
- ROS active oxygen species
- Antioxidants can be divided into those that exist naturally in the human body and those that are administered externally.
- Antioxidants that are naturally present in the human body include enzymes such as superoxide dismutase (SOD), glutathione, peroxidase and catalase. phytochemicals such as kaempferol, catechin and genistein; Vitamin E, vitamin C and beta carotene; And minerals such as selenium.
- SOD superoxide dismutase
- glutathione glutathione
- peroxidase and catalase
- phytochemicals such as kaempferol, catechin and genistein
- Vitamin E Vitamin C and beta carotene
- minerals such as selenium.
- Vitamin C L-ascorbic acid, ascorbate
- Vitamin C deficiency will cause diseases such as scurvy (Scurvy).
- Vitamin C in animals is an important factor in preventing wound healing and capillary bleeding, and is an important antioxidant in the body along with Vitamin A and Vitamin E.
- UVA UVA
- UVB ultraviolet B
- pollutants pollutants
- stress smoking, drinking, fatty foods, etc.
- cells may age or die.
- these substances reduce or degenerate the production of collagen and elastin, causing the skin to lose elasticity and wrinkles.
- antioxidants such as vitamin A, C, E
- the present invention has been made in view of the above necessity, and an object of the present invention is to provide a substance which acts as an antioxidant for antioxidant substances.
- the present invention provides an aptamer for preventing oxidation of an antioxidant substance.
- the present invention also provides an aptamer and an antioxidant complex obtained by combining an antioxidant with the aptamer of the present invention.
- the antioxidant is preferably a substance selected from the group consisting of vitamin C, vitamin A, retinol, vitamin E, astaxanthin, resveratinol, polyphenols, coenzyme Q10, peptides and oils.
- the antioxidant is not limited thereto.
- the present invention provides a cosmetic composition comprising the aptamer and antioxidant complex of the present invention as an active ingredient.
- the cosmetic composition preferably further comprises one or more of collagen, elastin, hyaluronic acid, and a peptide, but is not limited thereto.
- the cosmetic composition is to release the bound antioxidant (eg, vitamin C) when the structure of the aptamer changes according to the ultraviolet irradiation of the skin, or ATP according to the change in the temperature or acidity of the skin It is preferable to release vitamin C as the structure of the aptamer which binds thereto when the amount is changed, but is not limited thereto.
- the bound antioxidant eg, vitamin C
- the cosmetic composition of the present invention can use any raw material used in cosmetics regardless of any kind of extracts, active substances.
- green tea extract, licorice extract, medicinal extract, lettuce extract, golden extract, pueraria extract, red ginseng extract and apricot extract oil extract, orange extract, lemon extract, bamboo extract guava extract Rosemary Extract Cornus Extract, Ganoderma Lucidum Extract Ginkgo Extract Seoxiok-Yuksan Extract Consonant Extract, Moisturizing Chinese Quince Extract, White Lotus Extract, Paprika Extract, Aloe Extract, Scrubber Extract, Seaweed Extract, Antioxidant Carrot Extract, Soybean Extract, Grapefruit Extract Grape Seed Extract, Portulaca Extract, Caviar to Improve Wrinkles, Pomegranate, Ginseng Extract, Peach Extract for Skin Regeneration, Cheongung Extract, Centella asiatic Extract, Chamomile Extract, Licorice Extract, Ginseng Extract, Angelica Extract, Acne Good peppermint extract, triticale extract, eosungcho extract , Pe
- the present invention provides a nutritional supplement composition comprising the aptamer and antioxidant complex of the present invention as an active ingredient.
- vitamins for the nutritional supplements of the present invention include vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, vitamin B 6, vitamin B 12, thiamine, riboflavin, biotin, folic acid, niacin, pantothenic acid, mixtures thereof, and the like. to be.
- suitable mineral nutrients to be included in the dietary supplement composition are selected from sodium, potassium, calcium, magnesium, phosphorus, sulfur, chlorine, iron, copper, iodine, zinc, selenium, manganese, chromium, molybdenum, fluorine, cobalt, and compounds thereof They hold more than one element.
- herbs can also be used as nutritional supplements.
- herbs are selected from those having various medicinal or dietary supplement properties.
- herbs are aromatic plants or parts of plants that can be used for medicinal or flavoring purposes.
- the present invention provides a method for binding an aptamer to an antioxidant to maintain the reduced state of the antioxidant and prevent oxidation.
- the present invention like Vitamin C, vitamin A (Retinol), Vitamin E, Astaxanthin, Resveratinol, Polyphenol, Coenzyme Q10, Peptide, Oil, etc. Can be used to manufacture. This prevents the oxidation (corrosion) of the materials to ensure that they maintain the maximum effect they require. In addition, by releasing it under a target condition through apta-sensing, the effect can be increased as much as possible.
- Aptamer is a method of detecting a specific substance by using the three-dimensional structure of single strand DNA or RNA, which is similar to an antigen-antibody reaction, but has a much smaller substance, and can be controlled in various ways. This is an easy advantage. In addition, unlike antibodies, it is possible to synthesize aptamers that bind to very small chemicals (vitamins) and the like.
- Vitamin C is a water-soluble six carbon compound, which includes the 3-, 4- and 3-hydrocarbons of the furan ring and the semidehydroascorbic acid and dehydroascorbic acid, which are oxidized in the reduced form and biparticulate, respectively.
- Vitamin C which is combined with aptamers and maintains a reduced state, may be used in skin care compositions and nutritional supplements of various types of cream or hydrogel types including collagen, elastin, hyaluronic acid and peptides.
- the present invention also includes a method of slowly releasing Vitamin C according to various conditions of the skin (Aptasensing) through aptamers that react differently depending on the condition of the skin or external stimuli (eg ultraviolet rays or the temperature or acidity of the skin). For example, when the structure of the aptamer is changed by UV irradiation of the skin, the bound antioxidant (e.g., vitamin C) is released or the structure of the aptamer that binds when the amount of ATP changes according to the temperature or acidity of the skin is changed. And a method of releasing Vitamin C as a substitute.
- aptamers that react differently depending on the condition of the skin or external stimuli (eg ultraviolet rays or the temperature or acidity of the skin). For example, when the structure of the aptamer is changed by UV irradiation of the skin, the bound antioxidant (e.g., vitamin C) is released or the structure of the aptamer that binds when the amount of ATP changes according to the temperature or
- the aptamer of the present invention has an antioxidant effect of an antioxidant substance such as vitamin C, and the complex of the aptamer and antioxidant of the present invention may be, for example, contained in Vitamin C (ascorbic acid).
- Vitamin C ascorbic acid
- Aptamers that selectively bind to maintain the reduced state of Vitamin C to maintain its antioxidant function for a long time can be used in functional cosmetics and oral dietary supplements of various formulations.
- RNA or DNA RNA
- Figures 2 to 4 show that the three aptamers of the present invention prevent vitamin C oxidation by hydrogen peroxide solution
- 5 to 10 are graphs of dissociation constants (KD) for AA (ascorbic acid) and DHA (dehydroascorbic acid) of the aptamer of the present invention.
- Example 1 bound to reduced vitamin C Aptamer Military building
- SELEX systematic evolution of ligands by exponential enrichment
- aptamers were selected and subjected to the anti-oxidation experiment of vitamin C. After heating the aptamer dissolved in the annealing buffer to 95 o C and slowly lowering the temperature to room temperature, the secondary structure of the aptamer is mixed. The reaction was carried out for about 30 minutes. After oxidizing conditions by adding hydrogen peroxide solution, oxidation of L-ascorbic acid was measured by adding OPDA (o-phenylenediamine) as a fluorescent dye. DHA, the oxide of L-ascorbic acid, can be quantitatively analyzed by measuring the amount of fluorescence from DHA-OPDA produced by reaction with OPDA. Under the above conditions, the amount of fluorescence of DHA-OPDA was measured every 34 seconds for 25 minutes.
- OPDA o-phenylenediamine
- Example 3 of the present invention Aptamer ascorbic acid (AA) and dehydroascorbic acid ( DHA Steady-state solution dissociation constant (KD)
- Dissociation constants were determined using microscale thermophoresis (MST).
- This example includes the MST data and the calculated KD for the three aptamers in the assay buffer for both targets.
- All reagents containing ascorbic acid and dehydroascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO) and deionized water was treated with Chelex-100 resin for 1 hour to remove accidental metals prior to buffer preparation. After Chelex treatment, the water was sprayed with nitrogen gas for 10 minutes to minimize oxygen and then sealed and stored. This water was used for all aqueous solutions.
- Final buffer is 50 mM sodium acetate, pH 5.5, 1 mM MgCl 2, and 0.05% Tween-20, both AA and DHA were 153 pM (final) at e5 uM in buffer (for aptamers # 10 &# 12) Or at 1: 1 passage dilution at 1.53 nM (final) (Aptamer # 8) at 50 uM.
- the final concentration of each Cy5-conjugated aptamer is 20 nM.
Abstract
Description
Claims (8)
- 항산화 물질의 산화를 방지하는 압타머.Aptamers to prevent oxidation of antioxidants.
- 제1항에 있어서, 상기 항산화 물질은 비타민 C, 비타민 A, 레티놀, 비타민E, 아스타잔틴, 레스베라티놀, 폴리페놀, 코엔자임 Q10, 펩티드 및 오일로 구성된 군으로부터 선택된 물질인 것을 특징으로 하는 압타머.The method according to claim 1, wherein the antioxidant substance is selected from the group consisting of vitamin C, vitamin A, retinol, vitamin E, astaxanthin, resveratinol, polyphenol, coenzyme Q10, peptide and oil. Tamer.
- 항산화 물질과 제1항의 압타머를 결합시켜 얻어진 압타머와 항산화 물질 복합체.An aptamer and an antioxidant complex obtained by combining an antioxidant with the aptamer of claim 1.
- 제3항에 있어서, 상기 항산화 물질은 비타민 C, 비타민 A, 레티놀, 비타민E, 아스타잔틴, 레스베라티놀, 폴리페놀, 코엔자임 Q10, 펩티드 및 오일로 구성된 군으로부터 선택된 물질인 것을 특징으로 하는 압타머와 항산화물질 복합체.The method of claim 3, wherein the antioxidant substance is selected from the group consisting of vitamin C, vitamin A, retinol, vitamin E, astaxanthin, resveratinol, polyphenols, coenzyme Q10, peptides and oils. Tamer and Antioxidant Complex.
- 제3항 또는 제4항의 압타머와 항산화 물질 복합체를 유효성분으로 포함하는 화장료 조성물.Cosmetic composition comprising the aptamer and antioxidant complex of claim 3 or 4 as an active ingredient.
- 제5항에 있어서, 상기 화장료 조성물은 콜라젠, 엘라스틴, 히알루론산, 및 펩티드 중 하나 이상을 추가로 포함하는 것을 특징으로 하는 화장료 조성물.The cosmetic composition of claim 5, wherein the cosmetic composition further comprises one or more of collagen, elastin, hyaluronic acid, and a peptide.
- 제5항에 있어서, 상기 화장료 조성물은 피부의 자외선 조사에 따라 압타머의 구조가 바뀌면 결합된 항산화 물질이 방출되게 하거나, 피부의 온도나 산도의 변화에 따라 ATP양이 바뀔 때 이에 결합하는 압타머의 구조가 바뀜으로서 항산화 물질을 방출하는 것을 특징으로 하는 화장료 조성물.The aptamer of claim 5, wherein the cosmetic composition releases bound antioxidants when the structure of the aptamer changes according to ultraviolet irradiation of the skin, or binds to the ATP when the amount of ATP changes according to a change in skin temperature or acidity. Cosmetic composition, characterized in that to release the antioxidant substance by changing the structure of.
- 제3항의 압타머와 항산화물질 복합체를 유효성분으로 포함하는 영양 보조제 조성물.Nutritional supplement composition comprising the aptamer and antioxidant complex of claim 3 as an active ingredient.
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PCT/KR2017/004823 WO2017196077A1 (en) | 2016-05-12 | 2017-05-10 | Cosmetic and nutritional supplement using aptamer, and method for producing same |
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KR10-2016-0058022 | 2016-05-12 | ||
KR20160058022 | 2016-05-12 |
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PCT/KR2017/004823 WO2017196077A1 (en) | 2016-05-12 | 2017-05-10 | Cosmetic and nutritional supplement using aptamer, and method for producing same |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020096296A1 (en) * | 2018-11-06 | 2020-05-14 | 주식회사 넥스모스 | Method for preventing oxidation of polyphenol by means of aptamer, material thereof, and use thereof |
CN113164442A (en) * | 2018-10-25 | 2021-07-23 | (株)纳斯摩仕 | Composition for improving and treating depilation, hair damage and skin disease in animals comprising Aptamin-C as active ingredient |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108543079B (en) * | 2018-05-22 | 2019-12-24 | 广东药科大学 | Astaxanthin-collagen coupling compound and preparation method and application thereof |
WO2020096240A1 (en) * | 2018-11-06 | 2020-05-14 | 주식회사 넥스모스 | Dna aptamer specifically binding to glutathione and use thereof |
WO2020222506A1 (en) * | 2019-05-02 | 2020-11-05 | 주식회사 넥스모스 | Oral composition comprising aptamer as active ingredient |
Citations (4)
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US6235721B1 (en) * | 1999-02-17 | 2001-05-22 | Natreon Inc. | Stabilization of vitamin C |
US20070112064A1 (en) * | 2003-07-24 | 2007-05-17 | Lyles Mark B | Nucleic acid antioxidant compositions, methods for obtaining such compositions and formulations thereof |
WO2009031788A1 (en) * | 2007-09-04 | 2009-03-12 | Cotde, Ltd. | Cosmetic composition comprising vitamin c |
KR20120043167A (en) * | 2009-04-09 | 2012-05-03 | 시므라이즈 아게 | Compositions comprising trans-tert-butyl cyclohexanol as skin irritation-reducing agent |
-
2016
- 2016-10-19 WO PCT/KR2016/011741 patent/WO2017195951A1/en active Application Filing
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2017
- 2017-05-10 WO PCT/KR2017/004823 patent/WO2017196077A1/en active Application Filing
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US6235721B1 (en) * | 1999-02-17 | 2001-05-22 | Natreon Inc. | Stabilization of vitamin C |
US20070112064A1 (en) * | 2003-07-24 | 2007-05-17 | Lyles Mark B | Nucleic acid antioxidant compositions, methods for obtaining such compositions and formulations thereof |
WO2009031788A1 (en) * | 2007-09-04 | 2009-03-12 | Cotde, Ltd. | Cosmetic composition comprising vitamin c |
KR20120043167A (en) * | 2009-04-09 | 2012-05-03 | 시므라이즈 아게 | Compositions comprising trans-tert-butyl cyclohexanol as skin irritation-reducing agent |
Non-Patent Citations (2)
Title |
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HE, XIAOXIAO ET AL.: "ATP-Responsive Controlled Release System Using Aptamer -Functionalized Mesoporous Silica Nanoparticles", LANGMUIR, vol. 28, no. 35, 23 August 2012 (2012-08-23), pages 12909 - 12915, XP055106436 * |
KRYLOVA, SVETLANA M. ET AL.: "Mechanistic Studies on the Application of DNA Aptamers as Inhibitors of 2-oxoglutarate-dependent Oxygenases", JOURNAL OF MEDICINAL CHEMISTRY, vol. 55, no. 7, 3 March 2012 (2012-03-03), pages 3546 - 3552, XP055414197 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113164442A (en) * | 2018-10-25 | 2021-07-23 | (株)纳斯摩仕 | Composition for improving and treating depilation, hair damage and skin disease in animals comprising Aptamin-C as active ingredient |
WO2020096296A1 (en) * | 2018-11-06 | 2020-05-14 | 주식회사 넥스모스 | Method for preventing oxidation of polyphenol by means of aptamer, material thereof, and use thereof |
CN113439122A (en) * | 2018-11-06 | 2021-09-24 | (株)纳斯摩仕 | Method for preventing oxidation of polyphenols using aptamers, substances thereof and uses thereof |
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