KR100805355B1 - Cube-type natural seasoning base using hydrolysate of anchovy and a preparation method thereof - Google Patents
Cube-type natural seasoning base using hydrolysate of anchovy and a preparation method thereof Download PDFInfo
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- KR100805355B1 KR100805355B1 KR1020070021675A KR20070021675A KR100805355B1 KR 100805355 B1 KR100805355 B1 KR 100805355B1 KR 1020070021675 A KR1020070021675 A KR 1020070021675A KR 20070021675 A KR20070021675 A KR 20070021675A KR 100805355 B1 KR100805355 B1 KR 100805355B1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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Abstract
Description
도 1은 본 발명 된장국용 큐브형 천연조미료의 포장 전 모습을 보여주는 사진도이다.Fig. 1 is a photograph showing a state before packaging of a cube-type natural seasoning for miso soup according to the present invention.
도 2는 본 발명 된장국용 큐브형 천연조미료를 알루미늄 포장지를 이용하여 낱개로 포장한 후의 모습을 보여주는 사진도이다.FIG. 2 is a photograph showing a state after cube-type natural seasoning for miso soup according to the present invention is packaged individually using an aluminum wrapping paper.
본 발명은 멸치 가수분해물을 포함하는 큐브형 천연조미료 및 그 제조방법에 관한 것으로, 멸치에 가수분해효소를 첨가하여 교반한 후 상등액만을 취하고 이를 가열 농축하여 정치하였다가 원심분리하여 얻은 맑은 여액을 준비하고 여기에 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘을 첨가하고 락토오스 등의 결착제를 이용하여 큐브형으로 1회 분량의 규격에 맞게 절단하여 건조시킴으로써 제조된 큐브형 천연조미료 및 이의 제조방법에 관한 것이다.The present invention relates to a cube-type natural seasoning containing anchovy hydrolyzate and a method of preparing the same, wherein the hydrolysis enzyme is added to anchovy and stirred, and then only the supernatant is taken, which is then concentrated by heating and centrifuged to obtain a clear filtrate , And anchovy powder, powder, starch powder, kelp powder, doenjang or chili powder and chopped garlic are added thereto, and the resulting mixture is cut into cubes in a cube shape using a binder such as lactose and dried, Type natural seasoning and a method for producing the same.
우리나라 전 연안에서 어획되는 일시 다획성 어류인 멸치(Engraulis japonicus)는 청어목(Order Clupeiformes), 멸치과 (Family Engraulidae), 멸치속 에 속하며, 엑스분의 주성분인 비단백태 질소화합물의 함량이 높고, 그 중에서도 맛 성분을 내는 유리 아미노산, 핵산 관련 물질의 함량이 높다. Anchovies ( Engraulis japonicus ), which is a temporally multiply fish caught in the coastal area of Korea, belongs to the order Clupeiformes, Family Engraulidae and anchovy, and has high content of nontoxic nitrogen compounds, Among them, the contents of free amino acids and nucleic acid-related substances that give taste components are high.
멸치는 맛과 영양적인 면에서는 우수하지만, 다른 어종에 비하여 부패속도가 빠르기 때문에 어획량의 90% 이상이 자건품, 젓갈, 액젓의 원료로 사용되고 있으며, 대멸치를 원료로 한 멸치 액젓은 예부터 김치의 부재료, 간장대용, 무침이나, 절임용 등 다양한 용도로 사용되어 왔다. Anchovy is superior in terms of taste and nutrition, but more than 90% of the catch is used as a raw material for fish, fish and fish sauce because the decay rate is faster than other fish species. Anchovy sauce, And has been used for various purposes such as a substitute for soy sauce, a substitute for soy sauce,
이에 본 발명자는 상기와 같은 점을 감안하여 선도변화가 대단히 빠르고 일시적으로 많은 양이 어획되는 멸치를 신속하게 처리가공하여 기능성 조미식품소재를 개발하기 위하여 사용 효소를 선발하여 제조 과정 중의 산패도와 일반성분 그리고 색의 변화까지 분석하여 가장 좋은 최적 조건의 가수 분해물을 제조하고 상기 멸치 가수분해물에 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘을 첨가하고 락토오스 등의 결착제를 이용하여 큐브형으로 1회 분량의 규격에 맞게 절단하여 건조시킴으로써 관능미와 편리성이 개선된 큐브형 천연조미료를 제조할 수 있음을 발견하고 본 발명을 완성하였다.Accordingly, in view of the above, the inventor of the present invention has found that, in order to rapidly process anchovy, in which the change of the line is very fast and a large amount of which is caught temporarily, the enzyme used is selected to develop functional seasoning food materials, Then, the hydrolyzate having the best optimum conditions is prepared by analyzing the change of color, and anchovy powder, powder, starch powder, kelp powder, miso or chili powder and chopped garlic are added to the anchovy hydrolyzate and the binder such as lactose A cube-type natural seasoning having improved sensuality and convenience can be produced by cutting the cube-shaped cube-shaped one according to the standard and drying the cube-type natural seasoning. Thus, the present invention has been completed.
따라서, 본 발명의 목적은 멸치 가수분해물을 이용하여 제조된 관능미와 편리성이 개선된 큐브형 천연조미료를 제공하는 데 있다.Accordingly, an object of the present invention is to provide a cube natural seasoning improved in sensuality and convenience, which is produced using anchovy hydrolyzate.
본 발명의 또 다른 목적은 멸치 가수분해물을 이용하여 관능미와 편리성이 개선된 큐브형 천연조미료를 제조하는 방법을 제공하는 데 있다.It is another object of the present invention to provide a method for producing a cube-type natural seasoning improved in sensuality and convenience by using anchovy hydrolyzate.
본 발명의 상기 목적은 멸치에 가수분해효소를 첨가하여 교반한 후 상등액만 을 취하고 이를 가열 농축하여 정치하였다가 원심분리하여 얻은 맑은 여액을 준비하고 여기에 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘을 첨가하고 락토오스 등의 결착제를 이용하여 큐브형으로 1회 분량의 규격에 맞게 절단하여 건조시킴으로써 큐브형 천연조미료를 제조하고 이의 관능미를 평가함으로써 달성하였다.The above object of the present invention can be accomplished by adding a hydrolytic enzyme to anchovy and stirring, taking only the supernatant, concentrating it by heating, concentrating and centrifuging to prepare a clear filtrate. Anchovy powder, no powder, , Doenjang or red pepper powder and chopped garlic were added and the mixture was cut into cubes in a cube shape by using a binder such as lactose and dried to prepare a cube-type natural seasoning and evaluation of its sensuality.
이하 본 발명의 구성을 설명한다.Hereinafter, the configuration of the present invention will be described.
본 발명은 멸치 가수분해물을 포함하는 큐브형 천연조미료 및 그 제조방법을 제공한다.The present invention provides a cube-type natural seasoning containing anchovy hydrolyzate and a process for producing the same.
더욱 상세하게는 본 발명은 멸치에 가수분해효소를 첨가하여 교반한 후 상등액만을 취하고 이를 가열 농축하여 정치하였다가 원심분리하여 얻은 맑은 여액을 준비하고 여기에 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘을 첨가하고 락토오스 등의 결착제를 이용하여 큐브형으로 1회 분량의 규격에 맞게 절단하여 건조시킴으로써 제조된 큐브형 천연조미료 및 이의 제조방법을 제공한다.More particularly, the present invention relates to anchovy, which comprises adding anhydrous enzyme to anchovy and stirring, taking only the supernatant, concentrating it by heating and concentrating, and then centrifuging to prepare a clear filtrate. Anchovy powder, The present invention also provides a cube-type natural seasoning prepared by adding doenjang or red pepper powder and chopped garlic, and cutting the mixture into a cube-shaped one-size standard using a binder such as lactose, followed by drying, and a method for producing the same.
본 발명에서 멸치 가수분해물은 하기의 단계로 이루어진 방법으로 제조되는 것이 효율성 및 관능미 면에서 바람직하다:In the present invention, anchovy hydrolyzate is preferably produced from the viewpoints of efficiency and sensuality by the process comprising the steps of:
멸치를 초퍼로 마쇄하는 단계;Crushing anchovies with a chopper;
멸치 중량의 1~5 중량%에 해당하는 효소(Alcalase)를 첨가하는 단계;Adding an enzyme (Alcalase) corresponding to 1 to 5% by weight of anchovy weight;
50~70℃ 항온수조에 넣고 교반기로 1~5시간 동안 교반하는 단계;Placing in a constant temperature water bath at 50 to 70 캜 and stirring with a stirrer for 1 to 5 hours;
상등액만을 분리해내는 단계;Separating only the supernatant;
상기 단계에서 분리한 상등액을 1~5시간 동안 가열하여 농축하는 단계;Heating the supernatant separated in the step for 1 to 5 hours to concentrate the supernatant;
1~5시간 동안 정치하거나 원심분리하여 맑은 여액을 분리해내는 단계; 및Separating the clear filtrate by standing or centrifuging for 1 to 5 hours; And
상기 단계의 맑은 여액을 동결건조하는 단계.And lyophilizing the clear filtrate of the above step.
상기 가수분해물의 제조방법에서 마지막 단계에 소금을 적량 첨가하여 적당한 염도로 조절할 수 있다.In the method for producing the hydrolyzate, an appropriate amount of salt may be added at the final stage to adjust the salinity to an appropriate level.
본 발명의 큐브형 천연조미료는 하기 단계로 이루어진 방법으로 제조됨을 특징으로 한다:The cube-like natural seasoning of the present invention is characterized in that it is prepared by the following process:
멸치 가수분해물, 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘 및 결착제를 혼합하는 단계;Anchovy hydrolyzate, anchovy powder, no powder, ointment powder, kelp powder, doenjang or chili powder, chopped garlic, and a binder;
상기 단계의 혼합물을 큐브형으로 1회 분량의 규격에 맞게 절단하는 단계; 및Cutting the mixture of the above step into a cubic shape according to a one-time standard; And
50~70℃ 건조기에서 1~5시간 동안 건조하는 단계.And drying in a dryer at 50 to 70 ° C for 1 to 5 hours.
상기 건조된 각 큐브형 천연조미료는 알루미늄 포장지를 이용하여 낱개로 포장하여 제품화할 수 있다.Each dried cube-type natural seasoning can be packaged individually by using aluminum wrapping paper.
상기 큐브형 천연조미료의 멸치 가수분해물, 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘 및 결착제의 배합비율은 멸치 가수분해물 5~15중량부, 멸치분말 1~3중량부, 무분말 0.5~2중량부, 표고분말 0.5~2중량부, 다시마분말 0.5~2중량부, 된장 또는 고추가루 1~15중량부, 다진마늘 0.5~2중량부 및 결착제 0.5~2중량부인 것이 관능면에서 바람직하며 결착제의 경우 0.5중량부보다 적으면 큐브형으로 결착이 용이하지 않고 2중량부보다 많이 넣으면 관능미가 떨어지는 단점이 있다.The mixing ratio of the anchovy hydrolyzate, the anchovy powder, the non-powder, the ointment powder, the kelp powder, the soybean paste or the red pepper powder, the chopped garlic and the binder is 5-15 parts by weight of anchovy hydrolyzate, 0.5 to 2 parts by weight of powdered starch, 0.5 to 2 parts by weight of starch powder, 0.5 to 2 parts by weight of kelp powder, 1 to 15 parts by weight of miso or red pepper powder, 0.5 to 2 parts by weight of chopped garlic, If the amount is less than 0.5 parts by weight, the binder is not easily cube-shaped. If the binder is added in an amount of more than 2 parts by weight, the sensibility is lowered.
상기 큐브형 천연조미료에서 된장과 고추가루는 된장국용 천연조미료일 경우 된장을 선택하여 첨가하고 얼큰 국물용 천연조미료일 경우 고추가루를 선택하여 첨가함을 특징으로 한다.In the cube-type natural seasoning, soybean paste and red pepper powder are selected by adding soybean paste for natural seasoning for miso soup, and chili powder is selected for natural seasoning for soup stock.
본 발명에서 된장국용 큐브형 천연조미료의 가장 바람직한 구성은 멸치 가수분해물을 기준으로 멸치 가수분해물 10중량부, 멸치분말 2중량부, 무분말 1중량부, 표고분말 1중량부, 다시마분말 1중량부, 된장 4중량부, 다진마늘 1중량부 및 결착제 1중량부이다.The most preferable constitution of the cube-type natural seasoning for miso soup according to the present invention is that 10 parts by weight of anchovy hydrolyzate, 2 parts by weight of anchovy powder, 1 part by weight of no powder, 1 part by weight of starch powder, 4 parts by weight of miso, 1 part by weight of minced garlic and 1 part by weight of binder.
본 발명에서 얼큰 국물용 큐브형 천연조미료의 가장 바람직한 구성은 멸치 가수분해물 10중량부, 멸치분말 2중량부, 무분말 1중량부, 표고분말 1중량부, 다시마분말 1중량부, 고추가루 10중량부, 다진마늘 1중량부 및 결착제 1중량부이다.In the present invention, the most preferable constitution of the cube-type natural seasoning for ice-cream soup is 10 parts by weight of anchovy hydrolyzate, 2 parts by weight of anchovy powder, 1 part by weight of no powder, 1 part by weight of starch powder, 1 part by weight of kelp powder, 1 part by weight of chopped garlic and 1 part by weight of binder.
이하, 본 발명의 구성을 실시 예를 들어 상세히 설명하지만, 본 발명의 권리 범위가 하기 실시예에만 한정되는 것은 아니다. Hereinafter, the structure of the present invention will be described in detail by way of examples, but the scope of the present invention is not limited to the following examples.
실시예Example 1: 가수분해방법에 따른 멸치 1: Anchovy according to the hydrolysis method 가수분해물의Hydrolyzate 품질 조사 Quality survey
하기와 같이 조건을 달리하여 멸치 가수 분해물을 제조하여 품질을 검토하였다. 대조군으로는 시중에서 구입한 H사의 시판품인 액젓을 사용하였다.Anchovy hydrolyzate was prepared by varying the conditions as described below. As a control group, commercially available fish sauce commercially available from Company H was used.
효소 enzyme 열수병용법Combination of hydrothermal method
냉동 상태의 멸치를 해동한 후 마쇄하고 60℃ 항온수조에서 멸치육이 따뜻해질 때까지 교반하며 pH 7로 조정하였다. 그 후 멸치육 중량의 2%에 해당하는 효소(alcalase 2.4L)를 첨가하여 3시간 동안 분해하고 가수 분해액을 거즈로 거른 후 상등액만을 취하였다. 상등액을 3시간 동안 100℃로 가열하여 멸치 가수분해물 A를 얻었다.The frozen anchovy was thawed and then ground and adjusted to pH 7 with stirring in an incubation bath at 60 ° C until the anchovy meat was warm. After that, 2% of the anchovy meat was added (2.4L of alcalase), and the digestion was performed for 3 hours. The hydrolyzate was filtered with gauze, and only the supernatant was taken. The supernatant was heated to 100 ° C. for 3 hours to obtain anchovy hydrolyzate A.
열수효소Hydrothermal enzyme 병용법 Combination method
상기 효소 열수병용법을 응용하여 먼저 멸치를 3시간 동안 100℃에서 가열하여 열수 추출한 후 거즈로 거른 다음 멸치육 중량의 2%에 해당하는 효소(alcalase 2.4L)를 첨가하여 3시간 동안 분해하고 거즈로 맑은 여액만을 취하여 멸치 가수분해물 B를 제조하였다,The anchovy was heated for 3 hours at 100 ° C and then extracted with hot water. Then, the anchovy was subjected to hot water extraction, and then gauze was filtered. An enzyme (alcalase 2.4 L) corresponding to 2% of anchovy meat weight was added, The anchovy hydrolyzate B was prepared by taking only the clear filtrate.
효소열수Enzyme hydrothermal 침전법 Precipitation method
상기 효소 열수병용법과 동일한 방법으로 제조하되 여과공정을 생략하여 침전물까지 함께 혼합되어 있는 상태로 멸치 가수분해물 C를 제조하였다.The anhydrous hydrolyzate C was prepared in the same manner as in the method for the hydrothermal treatment of the enzyme except that the filtration step was omitted and the precipitates were mixed together.
효소 분해법Enzyme digestion method
냉동 상태의 멸치를 해동한 후 마쇄하고 물을 가하지 않고 멸치육 중량의 2%에 해당하는 효소(alcalase 2.4L)만을 첨가하여 8시간 동안 충분히 분해되도록 하여서 멸치 가수분해물 D를 제조하였다.The anchovy in the frozen state was thawed, and then anchovy hydrolyzate D was prepared by adding only 2% of anchors (2.4%) of anchovy meat weight without adding water, and allowing the anchovy to be sufficiently decomposed for 8 hours.
열수Heat number 추출법 Extraction method
냉동 상태의 멸치를 해동한 후 마쇄하고 물의 양을 다르게 첨가하여 3종류의 열수 추출법을 이용한 멸치 가수분해물 E, F, G를 제조하였다.Anchovy hydrolysates E, F and G were prepared by three kinds of hydrothermal extraction methods.
멸치 가수분해물 E는 멸치와 물을 동량 즉, 1:1의 중량비로 혼합하여 3시간 동안 100℃에서 가열하여 열수 추출한 후 거즈로 걸러 여과액을 취함으로써 얻었다.The anchovy hydrolyzate E was obtained by mixing the anchovy and water at the same weight ratio of 1: 1, heating at 100 ° C for 3 hours, extracting with hot water, filtering with gauze, and taking the filtrate.
멸치 가수분해물 F는 멸치와 물을 1:1/2의 중량비로 혼합하여 3시간 동안 100℃에서 가열하여 열수 추출한 후 거즈로 걸러 여과액을 취함으로써 얻었다.The anchovy hydrolyzate F was obtained by mixing anchovy and water at a weight ratio of 1: 1/2, heating the mixture at 100 DEG C for 3 hours, extracting hot water, filtering the mixture with gauze, and taking the filtrate.
멸치 가수분해물 G는 멸치만을 3시간 동안 100℃에서 가열하여 열수 추출한 후 거즈로 걸러 여과액을 취함으로써 얻었다.The anchovy hydrolyzate G was obtained by heating the anchovy at 100 ° C for 3 hours, extracting hot water, filtering it with gauze, and taking the filtrate.
부재료 첨가 효소 Enzyme-added enzyme 열수병용법Combination of hydrothermal method
냉동 상태의 멸치를 해동한 후 마쇄하고 60℃ 항온수조에서 멸치육이 따뜻해질 때까지 교반하며 pH 7로 조정하였다. 그 후 멸치육 중량의 2%에 해당하는 효소(alcalase 2.4L)를 첨가하여 3시간 동안 분해하고 가수 분해액을 거즈로 거른 후 상등액만을 취하였다. 상등액을 3시간 동안 100℃로 가열하여 얻은 멸치 가수분해물 10중량부에 무건조분말 1중량부, 표고버섯분말 1중량부, 다시마분말 1중량부 및 다진마늘 1중량부의 부재료를 첨가하여 12시간 동안 가열하여 거즈로 걸러 상등액 만을 취하여 멸치 가수분해물 H를 제조하였다.The frozen anchovy was thawed and then ground and adjusted to pH 7 with stirring in an incubation bath at 60 ° C until the anchovy meat was warm. After that, 2% of the anchovy meat was added (2.4L of alcalase), and the digestion was performed for 3 hours. The hydrolyzate was filtered with gauze, and only the supernatant was taken. 1 part by weight of non-dried powder, 1 part by weight of mushroom powder, 1 part by weight of kelp powder and 1 part by weight of chopped garlic were added to 10 parts by weight of the anchovy hydrolyzate obtained by heating the supernatant for 3 hours at 100 DEG C for 12 hours The anchovy hydrolyzate H was prepared by heating and filtering with gauze to take only the supernatant.
상기와 같이 제조한 가수 분해물들을 냉장 보관하여 시료로 사용하였다.The hydrolyzate prepared as described above was stored in a refrigerator for use as a sample.
일반성분으로는 수분, 회분, 조지방, 조단백, 탄수화물, 열량을 측정하였다. 수분은 상압가열건조법(105℃ 건조법), 회분은 직접회화법(550℃ 가열회화법), 조지방은 신속지방추출장치(자동화된 soxhlet`s 추출기)를 이용, 조단백 및 총질소량은 semimicro Kjeldahl법으로 A.O.A.C법에 의하여 측정하였다.Moisture, ash, crude fat, crude protein, carbohydrates and calories were measured as general components. The amount of crude protein and the amount of the crude protein were measured by the semimicro Kjeldahl method using the rapid drying method (105 ° C drying method), the direct method (550 ° C heating method), and the quick fat extraction method (automated soxhlet`s extractor) AOAC method.
그 결과를 하기 표 1에 나타내었다.The results are shown in Table 1 below.
멸치 가수분해물의 산패도 측정을 위해 휘발성염기질소, 아미노태 질소함량 등을 분석하여 산패도를 측정하였다. 아미노태질소(Amino nitrogen, AN)의 함량은 Formol 적정법에 따라 측정하였다. 시료 5 g을 마쇄하여 증류수 250 mL를 가하여 30분 동안 교반한 후 교반용액 25 mL에 미리 pH 8.4로 제조한 포름알데히드 용액 30 mL와 증류수 20 mL를 가하여 0.1 N-NaOH 용액으로 pH 8.4로 적정하였다. 같은 조작으로 포름알데히드 대신 증류수를 가하여 공시험을 실시하여 하기 식에 따라 아미노 질소 함량을 측정하였다.To measure acidity of anchovy hydrolysates, volatile basic nitrogen and amino nitrogen content were analyzed and acidity was measured. The content of Amino nitrogen (AN) was measured by the Formol titration method. 5 g of the sample was ground, 250 mL of distilled water was added, and the mixture was stirred for 30 minutes. 30 mL of formaldehyde solution prepared in advance to pH 8.4 and 20 mL of distilled water were added to 25 mL of the stirring solution and titrated to pH 8.4 with 0.1 N NaOH solution . By the same procedure, distilled water was added instead of formaldehyde to perform a blank test, and the amino nitrogen content was measured according to the following formula.
상기 식에서, A는 0.1N-NaOH 용액의 시료 적정량(mL), B는 0.1N-NaOH 용액의 공시험 적정량(mL), F는 0.1N-NaOH 용액의 농도계수를 의미한다.In the above equation, A represents a sample volume (mL) of a 0.1N NaOH solution, B represents a blank test volume (mL) of a 0.1N NaOH solution, and F represents a concentration coefficient of a 0.1N NaOH solution.
휘발성염기질소(Volatile Basic Nitrogen, VBN)함량은 Conway unit를 사용하는 미량 확산법으로 분석하였다. 시료 10g에 증류수 10mL와 10% TCA 20mL를 합하여 소량의 해사를 가해 마쇄 여과하고 5% TCA로 잔사를 세척하여 50mL로 정용하였다. 시료용액1 mL를 conway수기 외실에 넣고 내실에 H3BO3 1mL를 넣은 후 10% formalin 용액 1mL, K2CO3 1mL를 외실에 빠르게 주입하고 즉시 덮개를 덮은 후 시료용액과 K2CO3 용액이 잘 섞이도록 흔들어주고 37℃에서 1시간 정치한 다음 0.02N H2SO4 용액으로 적정하여 VBN가를 측정하였다. Volatile Basic Nitrogen (VBN) content was analyzed by the microdiffusion method using Conway unit. 10 g of the sample was combined with 10 mL of distilled water and 20 mL of 10% TCA, subjected to a small amount of marine filtration, and then subjected to washing with 5% TCA to wash the residue. Add 1 mL of sample solution into the outer chamber of the conway, add 1 mL of H3BO3 to the inner chamber, inject 1 mL of 10% formalin solution and 1 mL of K 2 CO 3 rapidly into the outer chamber, immediately cover the lid and mix the sample solution and K 2 CO 3 solution After rocking, the plate was incubated at 37 ° C for 1 hour and then titrated with 0.02NH 2 SO 4 solution to measure VBN.
상기 식에서, V1는 본시험의 0.02N H2SO4 용액의 적정소비량(mL), V2는 공시 험의 0.02N H2SO4 용액의 적정소비량(mL), F는 0.02N H2SO4 용액의 역가, D는 희석배수(50), S는 시료의 채취량(g), 0.28은 0.02N H2SO4 용액 1 mL에 상당하는 휘발성 염기질소량(mg)을 의미한다.Wherein, V1 is adequate consumption (mL), V2 is a proper consumption (mL) of 0.02NH 2 SO 4 solution in the blank test, F is the titer of 0.02NH 2 SO 4 solution of 0.02NH 2 SO 4 solution of the test, D is the dilution factor (50), S is the amount of sample (g), and 0.28 is the volatile basic nitrogen (mg) equivalent to 1 mL of 0.02NH 2 SO 4 solution.
TBA(Thiobarbituric acid)가는 Tarlagdis의 방법에 시료의 자동산화를 방지하기 위해 BHT를 첨가한 Pikul등(1983) 등과 같이 시료 10 g 가량을 정확히 취한 후 BHT(1000 mg/kg) 10 ml, 증류수 97.5 ml와 4 N HCl을 2.5 ml를 가하여 산성으로 한 뒤 증류하여 그 증류액을 50 ml 취하였다. 증류액 5 ml에 0.02 M TBA시액(2-Thiobarbituric acid, Sigma Chem. Co., USA)을 5 ml를 가하여 수욕상에서 35분간 진탕하여 발색시킨 후 바로 냉각하였다. 이용액을 분광광도계를 이용하여 538 nm에서 흡광도를 측정하였다.In order to prevent autoxidation of samples, 10 g of BHT (1000 mg / kg) and 97.5 ml of distilled water were added to the sample to prevent the autooxidation of TBA (Thiobarbituric acid) in the Tarlagdis method, as in Pikul et al. And 2.5 ml of 4 N HCl were added to the reaction mixture to make it acidic, followed by distillation, and 50 ml of the distillate was taken. 5 ml of 0.02 M TBA solution (2-Thiobarbituric acid, Sigma Chem. Co., USA) was added to 5 ml of the distillate, and the mixture was shaken for 35 minutes in a water bath and then immediately cooled. Absorbance was measured at 538 nm using a spectrophotometer.
TBA값은 지방산화를 평가하는 데 가장 널리 사용하는 방법 중의 하나인데, 1 kg의 유지 중에 함유되어 있는 malonaldehyde의 몰수로서 표시한다. 이 방법은 thiobarbituric acid 두 분자가 불포화지방의 산화생성물인 malonaldehyde 한 분자와 축합하여 붉은 색소(530 nm)를 생성하는 데 근거를 두고 있다.The TBA value is one of the most widely used methods for assessing fat oxidation, expressed as the number of moles of malonaldehyde contained in 1 kg of fat. This method is based on the fact that two molecules of thiobarbituric acid are condensed with one molecule of malonaldehyde, an oxidation product of unsaturated fats, to produce red pigment (530 nm).
그 결과를 하기 표 2에 나타내었다.The results are shown in Table 2 below.
식품공전에서 액젓의 아미노태질소량은 600 ㎎% 이상으로 규정하고 있는데, 상기 표 2를 통해 알 수 있듯이 효소를 첨가하여 제조한 효소열수병용법에 의한 멸치 가수분해물의 경우 1176 ㎎%, 열수효소병용법에 의한 멸치 가수분해물의 경우 798㎎%, 효소열수침전법에 의한 멸치 가수분해물의 경우 1176 ㎎%, 효소분해법에 의한 멸치 가수분해물의 경우 1195.6 ㎎%로 식품공전의 규정에 적합함을 확인할 수 있었다. 아미노태질소량이 높으면 액젓 고유의 감칠맛이 더 좋게 나오므로 효소열수병용법이나 효소열수침전법 또는 효소분해법이 관능면에서 바람직할 것으로 판단되었다.As can be seen from Table 2, the amino acid content of the fish sauce in the fermented food is more than 600 ㎎%. In the case of anchovy hydrolyzate by the enzyme hydrothermal method prepared by adding the enzyme, 1176 ㎎% It was found that the anchovy hydrolyzate by enzyme hydrolysis was 798㎎%, the anhydrous hydrolyzate by enzyme hydrothermal precipitation was 1176 ㎎%, and the anchovy hydrolyzate by enzyme digestion was 1195.6 ㎎%. there was. The higher the amount of amino acids, the better the intrinsic flavor of the sauce. Therefore, the combination of the enzymatic hydrothermal method and the enzymatic hydrothermal precipitation method or the enzymatic digestion method is preferable.
시료 제조시 열수 추출을 할 때 가열처리를 하였는데 이러한 가열처리는 휘발성염기질소의 일부가 당류와 반응하여 제품의 갈변을 유도할 수 있으며 이에 따라 휘발성염기질소량은 약간씩은 감소함을 알 수 있었다.It was found that some of the volatile basic nitrogen reacts with sugars to induce the browning of the product and thus the volatile basic nitrogen content decreases slightly.
아울러, 가열처리를 하여 시료 제조시 액젓에 함유된 아미노산 등 일부 함질소 성분이 열분해 및 축중합 반응을 통해 감소하므로 총질소량이 약간씩 감소함을 알 수 있었다.In addition, it was found that the amount of total nitrogen was slightly decreased because some nitrogenous components such as amino acids contained in the fish sauce were reduced through pyrolysis and polycondensation during the heat treatment.
상기 실험 결과를 토대로 효소열수병용법이 가장 효율적인 방법임을 확인할 수 있었으며, 이후 이러한 실험값을 바탕으로 효소열수병용법으로 제조한 멸치 가수분해물을 동결건조하여 분말화하고 이를 이용하여 큐브형 천연조미료를 제조하였다.Based on the above experimental results, it was confirmed that the method of combining the hydrothermal enzymes is the most efficient method. Based on these experimental values, the hydrolyzate of anchovy prepared by the enzyme hydrothermal method is lyophilized and powdered, and the cube-type natural seasoning is manufactured Respectively.
실시예Example 2: 된장국용 큐브형 천연조미료의 제조 2: Preparation of cube-type natural seasoning for miso soup
상기 실시예 1에서 효소열수병용법으로 제조한 후 동결건조하여 얻은 멸치 가수분해물, 멸치분말, 무분말, 표고분말, 다시마분말, 된장, 다진마늘 및 결착제(락토오스)를 하기 표 3의 배합비로 혼합한 후 큐브형으로 1회 분량의 규격에 맞게 성형 절단하고 60℃ 건조기에서 3시간 동안 건조하여 본 발명 된장국용 큐브형 천연조미료를 제조하였다. 하기 표 3에 기재된 조미료 2의 모습을 도 1에 나타내었다. 더 나아가, 제품화 시에는 상기 건조된 큐브형 천연조미료를 알루미늄 포장지를 이용하여 낱개로 포장하여 제품화하였다. 제품화된 큐브형 천연조미료의 모습을 도 2에 나타내었다. Anchovy hydrolyzate, anchovy powder, powder, starch powder, kelp powder, doenjang, chopped garlic, and binder (lactose) obtained by freeze-drying prepared by the method of the present invention in Example 1 were mixed at a blending ratio shown in Table 3 below After mixing, the mixture was shaped and cut into cubes according to the size of one time and dried in a drier at 60 DEG C for 3 hours to prepare a cube-type natural seasoning for miso soup of the present invention. The state of the seasoning 2 shown in Table 3 is shown in Fig. Furthermore, at the time of commercialization, the dried cube-type natural seasoning was individually wrapped and commercialized using an aluminum wrapping paper. Fig. 2 shows the appearance of a commercialized cube-type natural seasoning.
※상기 중량부는 멸치 가수분해물을 기준으로 한 것임
※ The above parts are based on anchovy hydrolyzate.
비교예Comparative Example 1: 멸치 1: Anchovy 가수분해물을The hydrolyzate 이용하지 않은 된장국용 큐브형 천연조미료의 제조 Manufacture of cube-type natural seasoning for miso soup
멸치분말 2중량부, 무분말 1중량부, 표고분말 1중량부, 다시마분말 1중량부, 된장 4중량부, 다진마늘 1중량부 및 결착제(락토오스) 1중량부를 혼합한 후 큐브형으로 1회 분량의 규격에 맞게 성형 절단하고 60℃ 건조기에서 3시간 동안 건조한 다음 알루미늄 포장지를 이용하여 낱개로 포장하여 된장국용 큐브형 천연조미료를 제조하였다.2 parts by weight of anchovy powder, 1 part by weight of no powder, 1 part by weight of oat powder, 1 part by weight of tallow powder, 4 parts by weight of miso, 1 part by weight of minced garlic and 1 part by weight of binder (lactose) The cube - shaped natural seasoning for miso soup was prepared by cutting and shaping it according to the standard of the amount of the ash, drying it for 3 hours in a drier at 60 ° C, and packaging it individually using the aluminum wrapping paper.
실험예Experimental Example 1: 된장국용 천연조미료의 관능평가 1: Sensory evaluation of natural seasoning for miso soup
시금치 100g씩을 준비하고 물 1L를 끓여 상기 시금치를 넣고 5분간 더 끓이다가 상기 실시예 1 및 비교예 1에서 제조한 큐브형 천연조미료 1개(2g)를 넣고 다시 5분간 더 끓여 시금치 된장국을 각각 제조하였다. 대조구로는 천연조미료 대신 시판중인 멸치다시다(C사 제품) 2g을 넣어 끓인 것을 사용하였다.100 g of spinach were prepared, 1 L of water was boiled, and the spinach was added. After boiling for 5 minutes, 1 cube-type natural seasoning (2 g) prepared in Example 1 and Comparative Example 1 was added and further boiled for 5 minutes to prepare spinach miso soup Respectively. As a control, 2 g of commercial anchovy (C) was used instead of natural seasoning and boiled.
관능평가는 20~50세의 남녀 각각 10명씩을 무작위로 추출하여 향, 맛, 색의 기호도를 9점 등급제(9=가장 좋다; 8=대단히 좋다; 7=보통으로 좋다; 6= 약간 좋다; 5=좋지도 싫지도 않다; 4=약간 싫다; 3=보통으로 싫다; 2=대단히 싫다; 1=가장 싫다)에 준하여 2회 평가한 뒤 최종적으로 응답한 점수를 합산하여 평균치를 구하였다. 관능평가 결과를 하기 표 4에 나타내었다. 관능검사 대상자들은 전체적으로 본 발명 천연조미료를 첨가하여 제조한 시금치된장국에서 훨씬 더 구수한 향이 나고 감칠맛이 난다고 평가하였다.Sensory evaluation was done by randomly extracting 10 persons from 20 to 50 years old male and 9 kinds of grades (9 = best; 8 = very good; 7 = fairly good; 6 = slightly good; 5 = I do not like it or not; 4 = I do not like it; 3 = I hate it, 2 = I hate it, 1 = I hate it the most). The results of sensory evaluation are shown in Table 4 below. The subjects of the sensory evaluation evaluated that the flavor of the spinach miso soup made by adding the natural seasoning of the present invention to the whole was much more fragrant and rich.
실시예Example 3: 3: 얼큰Ice 국물용 큐브형 천연조미료의 제조 Preparation of cube-type natural seasoning for soup
상기 실시예 1에서 효소열수병용법으로 제조한 후 동결건조하여 얻은 멸치 가수분해물, 멸치분말, 무분말, 표고분말, 다시마분말, 고추가루, 다진마늘 및 결착제(락토오스)를 하기 표 5의 배합비로 혼합한 후 큐브형으로 1회 분량의 규격에 맞게 성형 절단하고 60℃ 건조기에서 3시간 동안 건조하여 본 발명 얼큰 국물용 큐브형 천연조미료를 제조하였다. 더 나아가, 제품화 시에는 상기 건조된 큐브형 천연조미료를 알루미늄 포장지를 이용하여 낱개로 포장하여 제품화하였다. Anchovy hydrolyzate, anchovy powder, powder, starch powder, kelp powder, red pepper powder, chopped garlic and binder (lactose) obtained by freeze-drying the enzyme hydrothermal method in Example 1 were mixed in the ratio shown in Table 5 , Cut into cubes according to the standard of one part, and dried in a drier at 60 DEG C for 3 hours to prepare a cube-like natural seasoning for the soup of the present invention. Furthermore, at the time of commercialization, the dried cube-type natural seasoning was individually wrapped and commercialized using an aluminum wrapping paper.
비교예Comparative Example 2: 멸치 2: Anchovy 가수분해물을The hydrolyzate 이용하지 않은 Unused 얼큰Ice 국물용 큐브형 천연조미료의 제조 Preparation of cube-type natural seasoning for soup
멸치분말 2중량부, 무분말 1중량부, 표고분말 1중량부, 다시마분말 1중량부, 고추가루 10중량부, 다진마늘 1중량부 및 결착제(락토오스) 1중량부를 혼합한 후 큐브형으로 1회 분량의 규격에 맞게 성형 절단하고 60℃ 건조기에서 3시간 동안 건조한 다음 알루미늄 포장지를 이용하여 낱개로 포장하여 얼큰 국물용 큐브형 천연조미료를 제조하였다.2 parts by weight of anchovy powder, 1 part by weight of no powder, 1 part by weight of ointment powder, 1 part by weight of kelp powder, 10 parts by weight of red pepper powder, 1 part by weight of chopped garlic and 1 part by weight of binder (lactose) The cube - shaped natural seasoning for ice - cube soup was prepared by cutting and shaping according to the size of one time, drying in a drier at 60 ° C for 3 hours, and then wrapping it individually in an aluminum wrapping paper.
실험예Experimental Example 2: 된장국용 천연조미료의 관능평가 2: Sensory evaluation of natural seasoning for miso soup
쇠고기 양지머리 400g에 물 3L를 넣고 1시간 30분 동안 끓인 뒤 국물은 식혀 기름을 거둬 두고, 고기는 건져서 식힌 후 결대로 찢은 다음 참기름 10g, 국간장 20g, 고춧가루 16g, 다진마늘 20g을 넣고 양념하여 준비하였다.Add 3L of water to 400g of beef brisket and boil it for 1 hour and 30 minutes. After cooling the soup, cool the meat and cool it. After ripping it, tear it and cut it into pieces. Then add sesame oil 10g, soy sauce 20g, red pepper powder 16g and chopped garlic 20g Respectively.
고사리는 6cm 길이로 썰고, 토란줄기도 삶아 물기를 제거한 후 같은 크기로 썰어 준비하였다. 대파는 썰어서 끓는 물에 살짝 데친 다음 헹궈 준비하고, 느타리 버섯은 손으로 찢어 끓는 물에 데쳐 물기를 제거하고 숙주도 손질해 끓는 물에 데쳐 준비하였다.The bracken was cut into 6 cm lengths, and the taro stalks were boiled, the water was removed, and then cut into the same size and prepared. The green onions are sliced, boiled in a little water and then ready to rinse. The oyster mushrooms are torn by hand, boiled in water, removed from the water, prepared in the boiling water to prepare the host.
냄비에 고추기름 26g을 두르고 상기에서 준비한 고사리, 토란줄기, 대파, 느타리버섯, 숙주를 각각 100g씩 넣고 볶은 뒤 여기에 상기 고기를 삶고 남겨둔 국물을 붓고 1시간 동안 끓인 다음 상기 실시예 3 및 비교예 2에서 제조한 천연조미료 3개(6g)와 양념한 고기를 넣고 다시 10분 동안 끓여 육개장을 제조하였다. In a pan, 26 g of pepper oil was placed, and 100 g of each of the above prepared fern, turban stem, green onion, oyster, and host were added and roasted. The soup was boiled and boiled for 1 hour. Three natural seasonings (6 g) and seasoned meat were added to the mixture and the mixture was boiled for 10 minutes to prepare a six-pack.
대조구로는 천연조미료 대신 시판중인 멸치다시다(C사 제품) 6g을 넣어 끓인 것을 사용하였다.As a control, 6 g of commercially available anchovy (C) was used instead of natural seasoning.
관능평가는 20~50세의 남녀 각각 10명씩을 무작위로 추출하여 향, 맛, 색의 기호도를 9점 등급제(9=가장 좋다; 8=대단히 좋다; 7=보통으로 좋다; 6= 약간 좋다; 5=좋지도 싫지도 않다; 4=약간 싫다; 3=보통으로 싫다; 2=대단히 싫다; 1=가장 싫다)에 준하여 2회 평가한 뒤 최종적으로 응답한 점수를 합산하여 평균치를 구하였다. 관능평가 결과를 하기 표 6에 나타내었다. 관능검사 대상자들은 전체적으로 본 발명 천연조미료를 첨가하여 제조한 육개장에서 훨씬 더 감칠맛이 난다고 평가하였다.Sensory evaluation was done by randomly extracting 10 persons from 20 to 50 years old male and 9 kinds of grades (9 = best; 8 = very good; 7 = fairly good; 6 = slightly good; 5 = I do not like it or not; 4 = I do not like it; 3 = I hate it, 2 = I hate it, 1 = I hate it the most). The results of sensory evaluation are shown in Table 6 below. The subjects of the sensory test were evaluated to be much more tasty in the whiskers prepared by adding the natural seasoning of the present invention as a whole.
이상 상기에서 살펴본 바와 같이 본 발명 멸치 가수분해물을 포함하는 큐브형 천연조미료 및 그 제조방법은 멸치에 가수분해효소를 첨가하여 교반한 후 상등액만을 취하고 이를 가열 농축하여 정치하였다가 원심분리하여 얻은 맑은 여액을 준비하고 여기에 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘을 첨가하고 락토오스 등의 결착제를 이용하여 큐브형으로 1회 분량의 규격에 맞게 절단하여 건조시킴으로써 관능미가 개선되고 조리의 편리성을 줄 수 있는 큐브형 천연조미료를 제공할 수 있는 뛰어난 효과가 있으므로 식품산업상 매우 유용한 발명인 것이다.As described above, the cube-type natural seasoning containing the hydrolyzate of anchovy according to the present invention and the preparation method thereof are prepared by adding a hydrolyzing enzyme to anchovy and stirring, taking only the supernatant, concentrating it by heating and concentrating, And anchovy powder, no powder, oyster powder, kelp powder, doenjang or chili powder and chopped garlic were added thereto, and the mixture was cut into cubes according to the size of one time using a binder such as lactose and dried. And it is an extremely useful invention in the food industry because it has an excellent effect of providing a cube-type natural seasoning that can provide convenience of cooking.
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KR101285152B1 (en) | 2011-12-08 | 2013-07-11 | 경성대학교 산학협력단 | Functional Anchovy Seasoning And Manufacturing Method Thereof |
KR20200009516A (en) * | 2018-07-19 | 2020-01-30 | 김인경 | Cube-type natural seasoning sauce of portion for clear soup |
KR102126720B1 (en) * | 2019-08-22 | 2020-06-29 | 어업회사법인 주식회사 조혜정의 멸치연구소 | Method of seasoning bag for beanpaste soup with soybean paste of cube shape |
KR102205016B1 (en) * | 2020-08-20 | 2021-01-19 | 김인경 | Method for seasoning using natural binding agent and seasoning using natural binding agent thereof |
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KR101285152B1 (en) | 2011-12-08 | 2013-07-11 | 경성대학교 산학협력단 | Functional Anchovy Seasoning And Manufacturing Method Thereof |
KR20200009516A (en) * | 2018-07-19 | 2020-01-30 | 김인경 | Cube-type natural seasoning sauce of portion for clear soup |
KR102142186B1 (en) * | 2018-07-19 | 2020-08-06 | 김인경 | Cube-type natural seasoning sauce of portion for clear soup |
KR102126720B1 (en) * | 2019-08-22 | 2020-06-29 | 어업회사법인 주식회사 조혜정의 멸치연구소 | Method of seasoning bag for beanpaste soup with soybean paste of cube shape |
KR102205016B1 (en) * | 2020-08-20 | 2021-01-19 | 김인경 | Method for seasoning using natural binding agent and seasoning using natural binding agent thereof |
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