KR101136089B1 - Antihypertensive natural seasoning for Jjigae containing crab extract and preparation Thereof - Google Patents

Antihypertensive natural seasoning for Jjigae containing crab extract and preparation Thereof Download PDF

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KR101136089B1
KR101136089B1 KR1020090088496A KR20090088496A KR101136089B1 KR 101136089 B1 KR101136089 B1 KR 101136089B1 KR 1020090088496 A KR1020090088496 A KR 1020090088496A KR 20090088496 A KR20090088496 A KR 20090088496A KR 101136089 B1 KR101136089 B1 KR 101136089B1
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powder
weight
stew
natural
raw material
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KR20110030864A (en
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도정룡
김현구
임상동
김병삼
김병목
백수연
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한국식품연구원
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/40Shell-fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives

Abstract

본 발명은, 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료 및 그의 제조방법에 관한 것이다. The present invention relates to an antihypertensive natural seasoning for stew containing blue crab extract and a method for producing the same.

이는 특히, 고추분말 1-5중량%, 꽃게추출물 1-10중량%, 다시마분말 10-16중량%, 마늘분말 1-5중량%, 멸치분말 12-20중량%, 바지락분말 10-16중량%, 새우분말 12-20중량%, 쇠고기분말 10-20중량%, 표고버섯분말 10-20중량%, 그리고 황태분말 5-15중량%를 혼합하여 감칠맛이 풍부한 찌개용 천연조미료를 제조하는 방법에 관한 것이다. In particular, red pepper powder 1-5% by weight, blue crab extract 1-10% by weight, kelp powder 10-16% by weight, garlic powder 1-5% by weight, anchovy powder 12-20% by weight, clam powder 10-16% by weight , 12-20% by weight of shrimp powder, 10-20% by weight of beef powder, 10-20% by weight of shiitake mushroom powder, and 5-15% by weight of yellow powder, to prepare a natural seasoning for stew rich flavor .

이에 따라, 김치찌개, 된장찌개, 꽃게탕, 해물탕, 생선찌개, 두부고추장찌개, 등의 찌개에 천연의 감칠맛을 낼 수 있도록 하는 것이다.Accordingly, kimchi stew, soybean paste stew, crab soup, seafood soup, fish stew, tofu hot pepper stew, such as to make a natural rich flavor.

Description

꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료 및 그의 제조방법{Antihypertensive natural seasoning for Jjigae containing crab extract and preparation Thereof}Antihypertensive natural seasoning for Jjigae containing crab extract and preparation method thereof

본 발명은 김치찌개, 된장찌개, 꽃게탕, 해물탕, 생선찌개, 두부고추장찌개, 등의 찌게에 첨가하여 천연의 감칠맛을 낼 수 있도록 함으로써 기능성 천연조미료 생산 및 외식산업에 이용할 수 있는 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료 및 그의 제조방법에 관한 것이다. The present invention is added to the kimchi stew, soybean paste stew, crab soup, seafood soup, fish stew, tofu hot pepper stew, etc. to make a natural flavor, so as to contain a functional crab extract that can be used in the production of functional natural seasoning and catering industry It relates to an antihypertensive natural seasoning for stew and a preparation method thereof.

시중에 판매되고 있는 조미료는 대부분이 인공합성 화합물로 감칠맛을 내기위해 글루탐산나트룸 (MSG)과 핵산을 사용한 발효조미료다. Most seasonings on the market are fermented seasonings that use glutamate natrum (MSG) and nucleic acids to give a rich flavor to artificial synthetic compounds.

이와같은 인공합성 화합물의 문제점을 개선하도록 최근에는 천연 (자연)조미료를 표방하며 천연 소재 위주의 종합조미료 생산 기술이 크게 늘고 있으며, 일부 제품은 합성 성분을 배제하고 쇠고기, 각종 해산물, 양파, 마늘, 표고버섯 같은 천연 재료만을 사용하여 맛과 향을 내는데 주력하고 있다. In order to improve the problems of artificial synthetic compounds, in recent years, natural (natural) seasonings are being promoted, and natural seasoning-oriented synthetic seasoning technology is greatly increasing. Some products exclude synthetic ingredients, and include beef, various seafood, onions, garlic, It uses only natural ingredients such as shiitake mushrooms to focus on flavor and aroma.

그러나, 천연조미료가 과연 인공조미료 만큼의 맛과 향을 내기에는 부족하다는 시선이 많다. However, there are many eyes that natural seasonings are not enough to produce as much flavor and aroma as artificial seasonings.

특히, MSG와 핵산 성분은 강력한 감칠맛을 내기 때문으로 일부 조미료는 여전히 MSG성분을 제외시키지 못하고 있다. In particular, some seasonings still cannot exclude the MSG component because the MSG and nucleic acid component has a strong umami.

그나마 천연조미료가 인공조미료에 가까운 감칠맛을 내기 위해서는 글루탐산을 다량 함유한 쇠고기 함량을 높였기 때문이다. At the same time, natural seasonings increased the content of beef containing glutamic acid in order to give a rich taste close to artificial seasonings.

글루탐산은 20가지 아미노산 중 하나로 일반 조미료는 5-8%의 쇠고기를 함유하는데 반해 천연 조미료는 쇠고기 함량을 20%까지 높였다. Glutamic acid is one of 20 amino acids, while ordinary seasonings contain 5-8% beef, while natural seasonings increase beef content by 20%.

또한, 감칠맛을 내는 또 다른 성분인 핵산도 화학적으로 합성하는 대신 효모와 같은 천연 성분을 이용하였으며, 단맛은 설탕 대신 양파를 사용하고 있다. In addition, instead of chemically synthesizing nucleic acid, which is another umami-flavor, natural ingredients such as yeast were used. On the other hand, onions are used instead of sugar.

이러한, 인공조미료에 들어가는 MSG는 자율신경계를 망가뜨리는 등 문제점이 있어 MSG의 사용이 인체에 유해할 수 있다는 연구가 보고됨에 따라 자연적이고 친환경적인 천연조미료가 많은 주목을 받고 있다. As such, MSG in artificial seasonings has problems such as damaging the autonomic nervous system. As a result of research reports that the use of MSG may be harmful to the human body, natural and environmentally friendly natural seasonings have attracted much attention.

이에 따라 천연조미료 제조기술 뿐만 아니라, 나아가 용도를 구체적으로 하는 맞춤형 천연조미료의 개발에 대한 연구의 필요성이 대두되고 있으며, 특히 단백질과 정미성분이 풍부한 신선 식품 원료를 이용한 천연조미료의 성분과 효능을 좀 더 엄밀하게 분석하고 연구할 필요가 있다. Accordingly, the necessity of research on the development of natural seasonings that not only natural seasonings but also customized natural seasonings that specifically use them has emerged. Especially, the ingredients and efficacy of natural seasonings using fresh food ingredients rich in protein and seasoning ingredients are emerging. More rigorous analysis and research are needed.

이와 관련되어 천연재료를 이용한 조미료에 대하여 기술하고 있는 종래의 기술자료들을 살펴보면, In this regard, the conventional technical data describing seasonings using natural ingredients are described.

등록특허 제836586호에는 멸치나 다시마, 미역이나 새우등의 주재료로 이루어진 추출물을 금강송 톱밥이 충진된 관을 통해 여과시키는 방법으로 제조한 후 간장등의 부재료를 추가하여 동결건조하는 구성으로 이루어진 금강송을 이용한 천연 조미료 및 제조방법이, Patent No. 836586 uses an extract made of anchovies, kelp, seaweed, shrimp, etc. to extract extracts made of main ingredients through a tube filled with kumgangsong sawdust and then freeze-dried by adding subsidiary materials such as soy sauce. Natural seasonings and manufacturing methods

등록특허 제805355호에, 멸치와 가수분해효소를 첨가하여 교반한 후 상등액만을 취하고 이를 가열 농축하여 정치하였다가 원심분리하여 얻은 맑은 여액을 준비하고 여기에 멸치분말, 무분말, 표고분말, 다시마분말, 된장 또는 고추가루, 다진마늘을 첨가하고 락토오스 등의 결착제를 이용하여 큐브형으로 제조하는 멸치 가수분해물을 포함하는 큐브형 천연조미료 및 그 제조방법이, In Patent No. 805355, anchovies and hydrolase were added to the mixture, followed by stirring, and only the supernatant was taken. The mixture was left to heat and left to prepare a clear filtrate obtained by centrifugation. Anchovy powder, powder, shiitake powder, kelp powder Cube type natural seasoning comprising anchovy hydrolyzate prepared by adding a doenjang or red pepper powder, chopped garlic, and using a binder such as lactose into a cube,

등록특허 제862594호에, 진주조개육 엑기스 분말에 굴자숙농축액과 식염,유당, 모노소디움글루타메이트, 정제수, 효모추출물, 다시마 추출물 등을 포함하여 제조되는 풍미가 개선된 진주조개육 엑기스를 이용한 천연조미료의 제조방법이,In Korean Patent No. 862594, a natural seasoning using an improved pearl clam meat extract prepared from oyster meat concentrate and salt, lactose, monosodium glutamate, purified water, yeast extract, and kelp extract in pearl clam meat extract The manufacturing method of this,

등록특허 제732609호에, 주재료 다시마, 멸치, 표고버섯, 조개, 먹물을 제거하지 않은 오징어, 게, 마늘, 대파, 양파, 생강등을 분말구성한 주재료에 일반간장, 후추, 다랑어분말, 콩가루, 땅콩가루를 등을 혼합하는 구성으로 이루어진 천연 복합 조미료 등의 기술이 알려져 있다.In Patent No. 732609, the main ingredients are powdered kelp, anchovy, shiitake mushrooms, shellfish, squid, crab, garlic, green onions, onions, ginger, etc., without any soy sauce, pepper, tuna powder, soy flour, peanuts. BACKGROUND ART Techniques such as natural compounding seasonings composed of a mixture of powders and the like are known.

그러나, 상기와 같은 천연조미료는, 김치찌개, 된장찌개, 꽃게탕, 해물탕, 생선찌개, 두부고추장찌개, 등의 찌개에 등에 첨가하였을 경우 항고혈압 활성이 높은 천연의 감칠맛을 낼 수 없었다.However, the natural seasoning as described above, when added to the stew such as kimchi stew, doenjang stew, crab soup, seafood soup, fish stew, tofu cabbage stew, etc. could not give a natural rich flavor with high antihypertensive activity.

상기와 같은 종래의 문제점들을 개선하기 위한 본 발명의 목적은, 김치찌개, 된장찌개, 꽃게탕, 해물탕, 생선찌개, 두부고추장찌개, 등의 찌개에 사용하여 천연의 감칠맛을 낼 수 있도록 하는 찌개용 천연조미료의 제조 방법을 제공하는 데 있다.An object of the present invention for improving the conventional problems as described above, using natural kimchi stew, soybean paste stew, crab soup, seafood soup, fish stew, tofu cabbage stew, such as natural seasoning for stew to give a natural flavor It is providing the manufacturing method of the.

또한, 본 발명은, 찌개류에 첨가하여 사용할 때 항고혈압 활성이 높은 천연의 감칠맛을 낼 수 있도록 하는 찌개용 천연조미료의 제조 방법을 제공하는 데 있다.In addition, the present invention is to provide a method of producing a natural seasoning for stew so that when used in addition to the stew can be used to produce a natural rich flavor with high antihypertensive activity.

본 발명은 상기 목적을 달성하기 위하여, 고추분말, 꽃게추출물, 다시마분말, 마늘분말, 멸치분말, 바지락분말, 새우분말, 쇠고기분말, 표고버섯분말, 그리고 황태분말의 적정배합 비율을 결정하고, 이들을 혼합하여 감칠맛이 풍부하면서도 항고혈압 기능성을 나타내는 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료 를 제공한다.  In order to achieve the above object, the present invention, red pepper powder, blue crab extract, kelp powder, garlic powder, anchovy powder, clam powder, shrimp powder, beef powder, shiitake powder, and determine the appropriate mixing ratio of these powders, It provides a natural flavor for stew containing a crab extract that is rich in umami and yet exhibits antihypertensive functionality.

더하여, 본 발명은 고추분말 1-5중량%, 꽃게추출물 1-10중량%, 다시마분말 10-16중량%, 마늘분말 1-5중량%, 멸치분말 12-20중량%, 바지락분말 10-16중량%, 새우분말 12-20중량%, 쇠고기분말 10-20중량%, 표고버섯분말 10-20중량%, 그리고 황태분말 5-15중량%를 혼합하는 구성으로 이루어진 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료를 제공한다.In addition, the present invention is red pepper powder 1-5% by weight, blue crab extract 1-10% by weight, kelp powder 10-16% by weight, garlic powder 1-5% by weight, anchovy powder 12-20% by weight, clam powder 10-16 Antihypertensive for stew containing crab extract consisting of a mixture of weight%, shrimp powder 12-20% by weight, beef powder 10-20% by weight, shiitake mushroom powder 10-20% by weight, and yellow powder 5-15% by weight Provide natural seasonings.

그리고, 본 발명은 고추분말, 게추출물, 다시마분말, 마늘분말, 멸치분말, 바지락분말, 새우분말, 쇠고기분말, 표고버섯분말, 그리고 황태분말을 준비하면서 The present invention, while preparing red pepper powder, crab extract, kelp powder, garlic powder, anchovy powder, clam powder, shrimp powder, beef powder, shiitake mushroom powder, and yellow powder

쇠고기 분말은 쇠고기에 붙어있는 기름을 제거하고 파파인효소를 60℃에서 1시간 처리한 후 진공 동결 건조한 분말로 사용하며,Beef powder is used as a vacuum freeze-dried powder after removing the oil attached to the beef and processing papain enzyme for 1 hour at 60 ℃,

상기 원료의 혼합과 유효성분 및 맛 성분을 추출하기 위하여 10-15배의 정제수를 넣고 90-100℃에서 30-60분간 가열 추출하며,In order to extract the mixture and the active ingredient and taste components of the raw materials, 10-15 times purified water was added and heat-extracted at 90-100 ° C. for 30-60 minutes,

상기 추출한 원료를 6000 rpm에서 30분간 원심분리 하여 상층액을 얻고,The extracted raw material was centrifuged at 6000 rpm for 30 minutes to obtain a supernatant,

상기 추출한 원료는 40-50 brix가 되도록 가열농축 하고,The extracted raw material is heated and concentrated to 40-50 brix,

가열 농축한 원료는 분무건조 또는 진공동결 건조기를 사용하여 건조한 후 분쇄하고, The heat-concentrated raw material is dried using a spray drying or vacuum freeze dryer and then pulverized.

분말화한 원료는 80mesh 체를 사용하여 선별한 후 수분 투과도가 낮은 포장 용기를 사용하여 일정량 포장하는 구성으로 이루어진 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료의 제조방법을 제공한다.The powdered raw material provides a method for producing an antihypertensive natural seasoning for stew containing blue crab extract consisting of a predetermined amount of packaging using a packaging container with low moisture permeability after screening using an 80mesh sieve.

이상과 같이 본 발명에 의하면, 찌개의 맛을 돋우며 항고혈압 기능성을 나타내는 천연조미료를 제조하는 것이다.According to the present invention as described above, it is to produce a natural seasoning that enhances the taste of the stew and exhibits antihypertensive functionality.

또한, 김치찌개, 된장찌개, 꽃게탕, 해물탕, 생선찌개, 두부고추장찌개, 등의 찌개에 천연의 감칠맛을 낼 수 있는 것이다.In addition, kimchi stew, soybean paste stew, crab soup, seafood soup, fish stew, tofu hot pepper stew, etc., can give a natural flavor.

이하, 본 발명의 실시예를 구체적으로 설명한다.Hereinafter, embodiments of the present invention will be described in detail.

본 발명은, 찌개용으로 사용되는 천연조미료의 특성을 시험하기 위하여 항고혈압활성 및 관능검사용 시료를 제조한다.The present invention prepares a sample for antihypertensive activity and sensory test in order to test the characteristics of natural seasonings used for stew.

아래에서, 상기 항고혈압활성 및 관능검사용 시료의 제조 공정을 상세하게 설명하면 다음과 같다.Hereinafter, the manufacturing process of the antihypertensive activity and sensory test sample will be described in detail.

1) 원료의 준비 1) Preparation of raw materials

찌개용 천연조미료 제조용 원료로는 고추분말, 게추출물, 다시마분말, 마늘분말, 멸치분말, 바지락분말, 새우분말, 쇠고기분말, 표고버섯분말, 그리고 황태분말을 사용한다. Raw materials for manufacturing natural seasoning for stew are red pepper powder, crab extract, kelp powder, garlic powder, anchovy powder, clam powder, shrimp powder, beef powder, shiitake powder, and yellow powder.

이들 재료는 식품용 재료로서 품질이 우수한 것을 선별하여 사용한다. These materials are used for selecting foods having excellent quality as food materials.

그리고, 상기 꽃게추출물은 꽃게를 깨끗이 세척하여 2등분으로 절단하여 준비하고 게의 무게를 측정하여 3배가량의 정제수를 가하여 4시간 가열 추출하였다.The blue crab extract was prepared by washing the blue crab cleanly, cutting it into two portions, and weighing the crab, adding purified water of about 3 times, and extracting the mixture by heating for 4 hours.

계속하여, 상기 꽃게추출물을 냉각, 여과시켜 농축한 후, 진공 동결 건조하여 꽃게추출물을 제조하였다. Subsequently, the blue crab extract was cooled, filtered and concentrated, and then vacuum freeze-dried to prepare a blue crab extract.

특히, 쇠고기 분말의 제조에 있어서는 쇠고기에 붙어있는 기름을 제거하고 파파인 30,000효소를 60℃에서 1시간 처리한 후 진공 동결 건조하여 분말로 제조하였다.In particular, in the production of beef powder, the oil attached to the beef was removed and papain 30,000 enzyme was treated at 60 ° C. for 1 hour, and then vacuum-freeze-dried to prepare a powder.

이때, 상기 쇠고기의 가수분해효소인 파파인은, 플라보자임(flavourzyme) 500MG, 프로타멕스(protamex), 프로테아제(protease) NP, GC 106, 멀티펙트 누트랄(multifect nutral), 뉴트라아제(neutrase), 알칼라아제(alcalase) 효소 중에서 선택되는 어느 하나로 대체하여 사용할 수 있다.At this time, papain, a hydrolase of beef, is flavozyme 500MG, protamex, protease NP, GC 106, multifect nutral, neutrase , Alcalase (alcalase) can be used in place of any one selected from enzymes.

2) 가열 추출2) heat extraction

상기 원료의 혼합과 유효성분 및 맛 성분을 추출하기 위하여 10-15배의 정제수를 넣고 90-100℃에서 30-60분간 가열 추출한다.In order to extract the mixture and the active ingredient and taste components of the raw material, 10-15 times purified water was added and heat-extracted at 90-100 ° C. for 30-60 minutes.

3) 원심분리 3) Centrifugation

상기 추출한 원료를 6000 rpm에서 30분간 원심분리 하여 상층액을 얻는다.The extracted raw material is centrifuged at 6000 rpm for 30 minutes to obtain a supernatant.

4) 가열농축 4) heating concentration

상기 추출한 원료는 40-50 brix가 되도록 가열농축 한다.The extracted raw material is heated and concentrated to 40-50 brix.

5) 건조 5) drying

가열 농축한 원료는 분무건조 또는 진공동결 건조기를 사용하여 건조한다. The heat-concentrated raw material is dried using a spray drying or vacuum freeze dryer.

6) 분쇄 6) crushing

진공동결 건조한 원료를 분쇄한다. Vacuum freeze Grind dry material.

7) 선별 7) Screening

분말화한 원료는 80mesh 체를 사용하여 선별한다.The powdered raw material is screened using an 80mesh sieve.

8) 포장 8) Packing

수분 투과도가 낮은 포장 용기를 사용하여 일정량 포장한다. The container is packaged in a certain amount using a packaging container with low moisture permeability.

이하, 상기와 같이 준비된 시료를 다음의 실시 예 및 시험 예에 의하여 설명하고자 한다. Hereinafter, the sample prepared as described above will be described by the following examples and test examples.

그러나, 이들이 본 발명의 기술적 범위를 한정하는 것은 아니다.However, these do not limit the technical scope of the present invention.

<실시 예 1> 천연조미료에 사용한 원료 및 배합비Example 1 Raw Material Used in Natural Seasoning and Blending Ratio

정제수 1 L, 꽃게추출물 5 g, 다시마분말 15 g, 마늘분말 1 g, 멸치분말 15 g, 바지락분말 14 g, 새우분말 15 g, 쇠고기분말 15 g, 표고버섯분말 15 g, 그리고 황태분말 5 g을 90℃에서 60분 동안 가열추출한 후, 원심분리한 상층액을 50 brix가 되도록 가열 농축하여 농도를 조절한 후 진공 동결 건조하여 관능검사 및 항고혈압활성에 사용하였다.Purified water 1 L, blue crab extract 5 g, kelp powder 15 g, garlic powder 1 g, anchovy powder 15 g, clam powder 14 g, shrimp powder 15 g, beef powder 15 g, shiitake mushroom powder 15 g, and yellow powder 5 g After heat-extracting at 90 ° C. for 60 minutes, the supernatant was centrifuged to be heated to 50 brix, the concentration was adjusted, and vacuum lyophilization was used for the sensory test and antihypertensive activity.

<실시 예 2> 천연조미료에 사용한 원료 및 배합비Example 2 Raw Material Used in Natural Seasoning and Blending Ratio

정제수 1 L, 고추분말 1 g, 다시마분말 15 g, 마늘분말 1 g, 멸치분말 16 g, 바지락분말 16 g, 새우분말 16 g, 쇠고기분말 10 g, 표고버섯분말 10 g, 그리고 황태분말 15 g을 90℃에서 60분 동안 가열추출한 후, 원심분리한 상층액을 50 brix가 되도록 가열 농축하여 농도를 조절한 후 진공 동결 건조하여 관능검사 및 항고혈압활성에 사용하였다.1 L of purified water, 1 g of red pepper powder, 15 g of kelp powder, 1 g of garlic powder, 16 g of anchovy powder, 16 g of clam powder, 16 g of shrimp powder, 10 g of beef powder, 10 g of shiitake powder, and 15 g of yellow powder After heat-extracting at 90 ° C. for 60 minutes, the supernatant was centrifuged to be heated to 50 brix, the concentration was adjusted, and vacuum lyophilization was used for sensory and antihypertensive activity.

<실시 예 3> 천연조미료에 사용한 원료 및 배합비Example 3 Raw Material Used in Natural Seasoning and Compounding Ratio

정제수 1 L, 고추분말 1 g, 다시마분말 12 g, 마늘분말 1 g, 멸치분말 12 g, 바지락분말 12 g, 새우분말 12 g, 쇠고기분말 20 g, 표고버섯분말 20 g, 그리고 황태분말 10 g을 90℃에서 60분 동안 가열추출한 후, 원심분리한 상층액을 50 brix가 되도록 가열 농축하여 농도를 조절한 후 진공 동결 건조하여 관능검사 및 항고혈압활성에 사용하였다.1 L of purified water, 1 g of red pepper powder, 12 g of kelp powder, 1 g of garlic powder, 12 g of anchovy powder, 12 g of clam powder, 12 g of shrimp powder, 20 g of beef powder, 20 g of shiitake powder, and 10 g of yellow powder After heat-extracting at 90 ° C. for 60 minutes, the supernatant was centrifuged to be heated to 50 brix, the concentration was adjusted, and vacuum lyophilization was used for the sensory test and antihypertensive activity.

<실시 예 4> 천연조미료에 사용한 원료 및 배합비Example 4 Raw Material Used in Natural Seasoning and Compounding Ratio

정제수 1 L, 고추분말 1 g, 꽃게추출물 5 g, 다시마분말 16 g, 마늘분말 1 g, 멸치분말 16 g, 바지락분말 12 g, 새우분말 12 g, 쇠고기분말 16 g, 표고버섯분말 16 g, 그리고 황태분말 5 g을 90℃에서 60분 동안 가열추출한 후, 원심분리한 상층액을 50 brix가 되도록 가열 농축하여 농도를 조절한 후 진공 동결 건조하여 관능검사 및 항고혈압활성에 사용하였다.1 L of purified water, 1 g of red pepper powder, 5 g of blue crab extract, 16 g of kelp powder, 1 g of garlic powder, 16 g of anchovy powder, 12 g of clam powder, 12 g of shrimp powder, 16 g of beef powder, 16 g of shiitake mushroom powder, 5 g of yellow powder was heat-extracted at 90 ° C. for 60 minutes, and then, the supernatant was centrifuged and concentrated to 50 brix.

[표 1]. 관능검사 TABLE 1 Sensory evaluation

처리구Treatment flavor incense 기호도Symbol 실시 예 1Example 1 5.0 (0.2)5.0 (0.2) 6.5 (0.3)6.5 (0.3) 5.0 (0.4)5.0 (0.4) 실시 예 2Example 2 4.0 (0.5)4.0 (0.5) 6.0 (0.4)6.0 (0.4) 4.5 (0.3)4.5 (0.3) 실시 예 3Example 3 3.5 (0.5)3.5 (0.5) 5.5 (0.3)5.5 (0.3) 3.5 (0.5)3.5 (0.5) 실시 예 4Example 4 4.5 (0.4)4.5 (0.4) 5.5 (0.2)5.5 (0.2) 5.0 (0.2)5.0 (0.2)

*()는 표준편차임.* () Is the standard deviation.

상기 실험은 원료 배합 비율 및 제조공정을 달리 하여 제조한 찌개용 천연조미료의 관능 검사를 7점(매우 우수) 평점법으로 시행한 결과이다. The experiment is a result of the sensory test of the natural seasoning for stew prepared by different raw material blending ratio and manufacturing process by a 7-point (very good) grading method.

시험 예 1과 시험 예 4의 경우에도 관능검사 결과 기호도가 우수하였으나 시험 예 1의 경우, 향과 맛에서도 매우 우수하게 평가되었다.In the case of Test Example 1 and Test Example 4, the sensory test result showed that the acceptability was excellent, but in Test Example 1, the taste and taste were evaluated very well.

<시험 예> 천연조미료에 사용한 원료 및 천연조미료의 항고혈압 활성<Test Example> Antihypertensive Activity of Raw Materials Used in Natural Seasonings and Natural Seasonings

항고혈압활성은 Angiotensin-1 converting enzyme(ACE) 저해활성을 측정하여 분석하였다[표2]. Antihypertensive activity was analyzed by measuring Angiotensin-1 converting enzyme (ACE) inhibitory activity [Table 2].

천연조미료에 사용한 원료의 항고혈압활성은 쇠고기가 86.4로 가장 높았고, 꽃게, 새우, 바지락, 표고버섯, 마늘 순으로 나타났다.The antihypertensive activity of raw materials used in natural seasonings was highest in beef (86.4), followed by crab, shrimp, clams, shiitake mushrooms and garlic.

실시예에서 제조한 천연조미료의 항고혈압활성을 분석한 결과[표3] 실시예1이 61.6으로 가장 높았고, 실시예 4, 실시예 2, 실시예 3의 순으로 나타났다.As a result of analyzing the antihypertensive activity of the natural seasoning prepared in Example [Table 3] Example 1 was the highest as 61.6, followed by Example 4, Example 2, Example 3.

쇠고기를 파파인 30000으로 가수분해한 쇠고기의 항고혈압활성을 분석한 결과[표4] 20분 40분 60분 80분 가수분해 하였을 때 항고혈압활성은 74.7%, 82.5%, 86.4% 그리고 87.5%로 나타났다.The antihypertensive activity of beef hydrolyzed with papain 30000 was shown to be 74.7%, 82.5%, 86.4% and 87.5% when hydrolyzed 20 minutes 40 minutes 60 minutes 80 minutes. .

[표 2]. 천연조미료 원료 및 천연조미료의 항고혈압활성TABLE 2 Antihypertensive Activity of Natural Seasonings and Natural Seasonings

원료Raw material ACE저해활성(%)ACE inhibitory activity (%) 꽃게crab 57.557.5 쇠고기beef 86.486.4 새우shrimp 47.447.4 바지락Clam 43.143.1 표고버섯Shiitake mushrooms 26.126.1 다시마Kelp 3.23.2 마늘garlic 19.019.0 멸치Anchovy 2.92.9 황태Emperor 3.03.0

[표 3]. 천연조미료의 항고혈압활성TABLE 3 Antihypertensive Activity of Natural Seasonings

원료Raw material ACE저해활성(%)ACE inhibitory activity (%) 시험 예 1Test Example 1 61.661.6 시험 예 2Test Example 2 50.350.3 시험 예 3Test Example 3 47.847.8 시험 예 4Test Example 4 56.556.5

[표4]. 쇠고기 가수분해물의 항고혈압활성[Table 4]. Antihypertensive Activity of Beef Hydrolysates

기수분해 시간Hydrolysis time ACE저해활성(%)ACE inhibitory activity (%) 20분20 minutes 74.774.7 40분40 minutes 82.582.5 60분60 minutes 86.486.4 80분80 minutes 87.587.5

도1은 본 발명에 따른 찌개용 천연조미료의 제조 공정도이다.1 is a manufacturing process of the natural seasoning for stew according to the present invention.

Claims (3)

고추분말 1-5중량%, 꽃게추출물 1-10중량%, 다시마분말 10-16중량%, 마늘분말 1-5중량%, 멸치 12-20중량%, 바지락분말 10-16중량%, 새우분말 12-20중량%, 쇠고기분말 10-20중량%, 표고버섯분말 10-20중량%, 그리고 황태분말 5-15중량%를 혼합하는 구성으로 이루어진 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료Red pepper powder 1-5%, blue crab extract 1-10%, kelp powder 10-16%, garlic powder 1-5%, anchovy 12-20%, clam powder 10-16%, shrimp powder 12 Anti-hypertensive natural seasoning for stew containing blue crab extract consisting of 20% by weight, 10-20% by weight of beef powder, 10-20% by weight of shiitake mushroom powder, and 5-15% by weight of yellow powder 제1항에 있어서, 상기 쇠고기 분말은, 플라보자임(flavourzyme) 500MG, 프로타멕스(protamex), 파파인(papain) 30,000, 프로테아제(protease) NP, GC 106, 멀티펙트 누트랄(multifect nutral), 뉴트라아제(neutrase), 알칼라아제(alcalase) 효소 중에서 선택되는 어느 하나 이상의 가수분해효소로서 가수분해되는 것을 특징으로 하는 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료The method of claim 1, wherein the beef powder, Flavozyme (500 mg), protamex (protamex), papain (papain) 30,000, protease (protease NP), GC 106, multifect nutral (multifect nutral), Anti-hypertensive natural seasoning for stew containing blue crab extract, characterized in that it is hydrolyzed as at least one hydrolase selected from neutrase and alcalase enzymes 제1항의 조성으로 이루어진 재료를 준비하면서 While preparing the material of the composition of claim 1 쇠고기분말은 쇠고기에 붙어있는 기름을 제거하고 파파인 30,000효소를 투입하여 60℃에서 1시간 처리한 후 진공 동결 건조시켜 준비하며, 꽃게추출물은 게의 무게를 측정하여 3배가량의 정제수를 가하여 4시간 가열 추출하여 준비하고,Beef powder is prepared by removing oil attached to beef, adding papain 30,000 enzyme, treating it for 1 hour at 60 ° C, and vacuum freeze-drying.The crab extract is weighed by adding 3 times the amount of purified water for 4 hours. Prepared by heat extraction, 제1항의 조성으로 이루어진 상기 원료의 혼합과 유효성분 및 맛 성분을 추출하기 위하여 10-15배의 정제수를 넣고 90-100℃에서 30-60분간 가열 추출하며,In order to extract the mixture of the raw material and the active ingredient and the taste component of the composition of claim 1, 10-15 times purified water, and extracted by heating for 30-60 minutes at 90-100 ℃, 상기 추출한 원료를 6000 rpm에서 30분간 원심분리 하여 상층액을 얻고,The extracted raw material was centrifuged at 6000 rpm for 30 minutes to obtain a supernatant, 상기 추출한 원료는 40-50 brix가 되도록 가열농축 하고,The extracted raw material is heated and concentrated to 40-50 brix, 가열 농축한 원료는 분무건조 또는 진공동결 건조기를 사용하여 건조한 후 분쇄하고, The heat-concentrated raw material is dried using a spray drying or vacuum freeze dryer and then pulverized. 분말화한 원료는 80mesh 체를 사용하여 선별한 후 수분 투과도가 낮은 포장 용기를 사용하여 일정량 포장하는 구성으로 이루어진 꽃게추출물을 함유하는 찌개용 항고혈압 천연조미료의 제조방법Powdered raw material is screened using a 80mesh sieve and then prepared using a packaging container with low moisture permeability to produce a certain amount of anti-hypertensive natural seasoning for stew containing blue crab extract.
KR1020090088496A 2009-09-18 2009-09-18 Antihypertensive natural seasoning for Jjigae containing crab extract and preparation Thereof KR101136089B1 (en)

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KR100701824B1 (en) * 2005-08-26 2007-04-02 송운학 a manufacturing method of chitosan kimchi with sweet pumpkin and blue crab
KR20080092649A (en) * 2007-04-13 2008-10-16 양영남 Famous abalone flavor

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