KR100787175B1 - A herbal mixture extract comprising Atractylodis Rhizoma Alba, Gastrodia elata, Aurantii nobilis pericarpium, Poria cocos, Crataegur, Siegesbeckia glabrescens Makino, and food supplement comprising the same for prevention and treatment of Arteriosclerosis - Google Patents

Abstract

The present invention is an extract for the prevention and treatment of atherosclerosis and related diseases of herbal mixtures, including baekchul, cheonma, dermis, bokyeong, hawthorn and haejugo, and for the prevention and treatment of atherosclerosis and related diseases comprising the same as an active ingredient. It relates to a health food composition, it is preferable to extract using water or ethanol as a solvent in the preparation of the extract. In addition, the present invention is an extract for preventing and treating atherosclerosis and related diseases of the Chinese herbal medicine mixture, which is selected by adding one or more selected from the group consisting of rhubarb, and the arteriosclerosis comprising the same as an active ingredient It provides a health food composition for the prevention and treatment of related diseases.

As a result of experiments on the extract of the present invention, the extract inhibited about 2 times the proliferation of vascular smooth muscle cells increased by platelet derived growth factor (PDGF) compared to the positive control PDGF alone group, In addition, when the extract was treated with vascular smooth muscle cells at a concentration of 1 mg / ml for 24 hours, it showed a significant cell migration inhibition effect of about 50% compared to the positive control. Therefore, the extract according to the present invention can be usefully used as an agent for preventing and treating atherosclerosis and related diseases.

 Eruption, shunt, vascular smooth muscle cell (SMC), platelet derived growth factor (PDGF), proliferation, migration, arteriosclerosis

Description

Extracts for the prevention and treatment of atherosclerosis and related diseases of herbal mixtures including baekchul, cheonma, dermis, bokyeong, hawthorn and haejugo, and health food compositions for the prevention and treatment of atherosclerosis and related diseases containing the same as an active ingredient {A herbal mixture extract comprising Atractylodis Rhizoma Alba, Gastrodia elata, Aurantii nobilis pericarpium, Poria cocos, Crataegur, Siegesbeckia glabrescens Makino, and food supplement comprising the same for prevention and treatment of Arteriosclerosis}

1 is a graph showing the effect of the extract on the vascular smooth muscle cells according to the concentration of the extract according to the present invention and measuring the effect on cell viability.

Figure 2 is a graph showing the effect of inhibiting the extract according to the present invention vascular smooth muscle cell proliferation increased by platelet derived growth factor.

Figure 3 is a graph showing the effect of the extract according to the invention on the migration of vascular smooth muscle measured by Transwell migration ability analysis method.

Figure 4 is a photograph showing the effect of the extract according to the present invention to inhibit vascular smooth muscle cell migration increased by platelet-derived growth factor.

The present invention is an extract for the prevention and treatment of atherosclerosis and related diseases of the herbal medicine mixture including baekchul, cheonma, dermis, bokyeong, hawthorn and haejugo, and for the prevention and treatment of atherosclerosis and related diseases comprising the same as an active ingredient It relates to a health food composition.

Atherosclerosis is a disease in which thickening or tissue degeneration occurs in the artery wall, causing it to stiffen. The inner lining of the arteries becomes thick, narrowing the inner diameter, and supplying oxygen and various nutrients to the coronary arteries, brain, and lower limbs. Blood flow disorders appear in the arteries, one of the major diseases that occur with aging. Atherosclerosis is a process in which fibrosis occurs around an atherosclerosis caused by excessive cell proliferation in the artery's lining, thereby stiffening blood vessels. Atherosclerosis is sometimes referred to as atherosclerosis.

Atherosclerosis begins around the age of 10 years and blood flow disorders appear in late 40 years. Sometimes, the fibrous membrane ruptures to cause blood clots in the blood vessels, and bleeding occurs within the atherosclerosis, which causes the disease to narrow rapidly. Atherosclerosis is more likely to occur in the cerebral artery or coronary arteries. In the case of cerebral arteriosclerosis, headache, dizziness, and mental disorders are indicated and cause encephalopathy, and in the case of coronary arteriosclerosis, pain and arrhythmia in the heart cause angina and myocardial infarction. It is known to become. In addition, this causes heart disease, cerebral hemorrhage, and diseases caused by arteriosclerosis are emerging as the leading cause of death in modern society, especially among men in their 50s and 60s. In the West, it is a common cause of mortality, and according to the National Statistical Office's announcement, it increased from 20% in 1995 to 73% in 2000, and the atherosclerosis-related mortality rate increased about 50% in Korea. In addition, atherosclerosis is deeply related to the development and progression of diseases such as metabolic syndrome such as obesity (overweight), blood clots, hypercoagulation and thrombolytic conditions (arteries and veins), including hypertrophy, and dyslipidemia. Causes or worsens chronic complications.

Arteries are divided into aorta, middle and small arteries, and are composed of three layers of inner, middle, and outer membranes. In particular, atherosclerosis occurs mainly in elastic and muscular arteries. Vascular smooth muscle cells that function as vasoconstrictors and dilates can migrate and proliferate into the lining when stimulated and have receptors for LDL. In normal conditions, the proliferation is controlled by a very strict mechanism, but in abnormal cases it can overproliferate and cause vascular disease. In addition, vascular smooth muscle cells cause vascular restenosis in the treatment of ischemic heart disease such as angina pectoris and myocardial infarction. To prevent this, vascular smooth muscle cells are used to inhibit smooth muscle cell proliferation.Antioxidants, antiplatelets, vasodilators, anticoagulants Although administration of the back is performed, it does not show clinically sufficient effect.

Banha, Baekchul, Cheonma are the main components of Banha Baekchulcheonmatang, and they are prescribed for the symptom of headaches caused by nasal injuries, prudent acid, nausea, cold limbs, vomiting, and vertigo. In recent years, it has been applied to the prevention and treatment of cardiovascular diseases due to the dehumidification. Pathologically, weakness of the stomach is caused by intragastric infiltration and venom. Also, because of the impairment of blood flow, it is associated with the condition of cardiovascular disease. It is expected to improve the disease and lower blood pressure.

Therefore, the present inventors have studied the efficacy of treating arteriosclerosis by extracting a mixture of herbal preparations including baekchul, cheonma, dermis, bokyeong, hawthorn and haejugo, the extract according to the present invention is a platelet derived growth factor (PDGF) The present invention was found to have an effect of inhibiting the growth and vascular activity of vascular smooth muscle cells increased by).

Accordingly, it is an object of the present invention to uncover the objective efficacy of inhibiting the proliferation and lipogenic activity of vascular smooth muscle cells against extracts of herbal mixtures including baekchul, cheonma, dermis, bokyeong, hawthorn and hunger, preventing the prevention of atherosclerosis and related diseases. And to provide a therapeutic extract.

Another object of the present invention is to provide a health food composition for the prevention and treatment of atherosclerosis and related diseases, including extracts of herbal mixtures including baekchul, cheonma, dermis, bokyeong, hawthorn and hunger.

Another object of the present invention is an extract for the prevention and treatment of atherosclerosis and related diseases of the Chinese herbal medicine mixture, in addition to the herbal medicine mixture, by selecting one or more from the group consisting of rhubarb, and further comprising the same as an active ingredient To provide a health food composition for the prevention and treatment of atherosclerosis and related diseases.

In order to achieve the above object, the present invention provides an extract for the prevention and treatment of atherosclerosis and related diseases of the herbal medicine mixture, including baekchul, cheonma, dermis, bokyeong, hawthorn and hunger. The present invention also provides a health food composition for the prevention and treatment of atherosclerosis and related diseases comprising the extract as an active ingredient.

In addition, the present invention is an extract for preventing and treating atherosclerosis and related diseases of the Chinese herbal medicine mixture, which is further selected by selecting one or more from the crowd consisting of rhubarb, and an artery comprising the same as an active ingredient, in contrast to the herbal medicine mixture. It provides a health food composition for the prevention and treatment of sclerosis and related diseases.

It is preferable to extract using water or ethanol as a solvent in the preparation of the extracts.

EMBODIMENT OF THE INVENTION Hereinafter, the structure of this invention is demonstrated concretely.

The present invention provides extracts for the prevention and treatment of atherosclerosis and related diseases of herbal mixtures, including baekchul, cheonma, dermis, Bokryeong, hawthorn and hunger.

The pharmacological properties of each ingredient medicine of the composition of the present invention are as follows.

Atractylodis Rhizoma Alba used in the present invention causes rooting of perennial plant inserts belonging to the Asteraceae family, and is a fragrant conditional agent used in oriental medicine to supplement the spleen, supplemented with sputum and bobby prescription, soothing, diuretic, cold, nourishing, ante effect, Analgesic, anti-inflammatory, hypoglycemic effect, lowering blood pressure, diuretic effect is effective.

Gastrodia elata is a perennial herb of Orchidaceae. It is known for its effects on neurasthenia, pain, headache, stroke and hypertension. It is also used for kidney disease and diabetes.

The dermis (Aurantii nobilis pericarpium) is a tangerine peel, ripened such as Citrus Auranticum L. or C. Junos and C. nobilis belonging to the Sanaceae or Rutaceae family. It is peeled and dried in the sun, and it is known that the longer it stays in motion therapy, the ingredients contain essential oils and flavonoids, hesperidin, which are widely used in fragrant dry stomach, Jinhae, sputum, stomachache, abdominal pain, etc. It is a kind of herbal medicine with stability.

Poor cocos (Poria cocos) is parasitic in the roots of the tree, the size of the fungal nucleus (菌核) is 10 ~ 30cm and white is called the white baekbokyeong (白 茯 笭), red is called the red bokbokyeong (赤 茯 笭). It is an herbal medicine and is effective in tonic, diuretic and soothing. It is used for kidney disease, cystitis and urethritis.

Crataegur is a deciduous arborescent belonging to the family Rosaceae, and its components include hyperside, quercetin, and oleanol. Fruits are spherical or obovate, and hawthorn. The drug is used in stomach, digestion, tonic, high blood pressure, dysmenorrhea, etc.

Siegesbeckia glabrescens Makino is an annual herb of Asteraceae, and its components include diterpentauroside, daturin, alkaloids, saponins, and essential oils, and are effective in lowering blood pressure, swelling, customs, and stroke.

Cell experiments were carried out to investigate the inhibitory effect of vascular smooth muscle cells of the medicinal herb extract according to the present invention. Table 1 shows the results of the extract of the present invention observed the efficacy of inhibiting the migration ability of vascular smooth muscle cells. As a result, vascular smooth muscle cells migrated up to 95% by PDGF, but the extract of the present invention exhibited 42% vascular smooth muscle cell migration at a concentration of 1 mg / ml, indicating an effect of inhibiting cell migration by PDGF.

PDGF (5 ng / ml) + + Extract concentration of Example 1 (mg / ml) - One Cell migration distance (%) 12 hours 95 42

The present invention provides an extract for the prevention and treatment of atherosclerosis and related diseases of the Chinese herbal medicine mixture in which at least one selected from the group consisting of rhubarb is further mixed with the herbal medicine mixture.

Vanellia (Pinellia termate) is a perennial herb that removes all cork layers and uses tubers. It is known to have the effects of clay, diuresis, intraocular pressure drop, antitussive expectoration, anti-secretion, antiarrhythmic and anticancer activity.

Coptis Rhizoma is a perennial herb belonging to the Ranunculaceae, and its main ingredients include berberine, copticine, borenine, palmin, and yaterorhizine. It is used as a high-density gastrointestinal pill, and the main ingredient is berberine, which has an antibacterial effect of inhibiting the growth of intestinal bacteria, and is used in herbal medicine for gastrointestinal diseases, bleeding, arteriosclerosis, inflammation, and mental anxiety.

Cell experiments were carried out to investigate the effect of inhibiting the proliferation and migration activity of vascular smooth muscle cells of the medicinal herb extract of the present invention containing both halves and barberry. First, in the experiment on vascular smooth muscle cell proliferation, the percentage of cell proliferation increased by 60% to 160% when PDGF alone was administered when 100% of cells proliferated at 48 hours in the negative control group. On the other hand, when the co-administration of the extract of the present invention including both PDGF and halves and rhubarb shows about 90-100% proliferation of vascular smooth muscle cells similar to the negative control, compared with the positive control PDGF alone group. Vascular smooth muscle cell proliferation was significantly suppressed (FIG. 2).

In addition, as a result of measuring the efficacy of the extract of the present invention containing both halves and rhubarb on the migration of vascular smooth muscle cells by using the transwell migration assay, PDGF alone was administered 48 hours after the smooth muscle cell migration activity. When expressed as 100%, the group administered with the concentration of the extract of the present invention containing both PDGF and half and rhubarb appeared in 80% -40%. Therefore, the extract containing both halves and rhubarb was shown to inhibit the migration of vascular smooth muscle cells by PDGF in a concentration-dependent manner (Fig. 3).

In addition, the effect of inhibiting the migration activity of vascular smooth muscle cells in the herbal extracts of the present invention including both halves and rhubarb was measured by wound healing assay, and vascular smooth muscle cells 24 hours after PDGF alone was administered. When the induction activity of 100%, the group administered with the extract of the present invention containing both PDGF and half and rhubarb showed about 20-50% induction activity and the migration of vascular smooth muscle cells by PDGF It was shown to inhibit (Table 2, Figure 4). Table 2 shows the results of quantifying the results of statistical treatment after administering the extracts of the present invention including both halves and rhubarb and repeating the effects of increased platelet-derived growth factor on vascular smooth muscle cell migration inhibition 3-4 times. Table.

PDGF (5 ng / ml) + + + + Extract concentration of Example 1 (mg / ml) - 0.1 0.5 One  Cell migration distance (%) 12 hours 95 95 59 36 24 hours 100 100 76 52

Therefore, the extracts according to the present invention effectively inhibit the migration and proliferation of vascular smooth muscle cells, and thus, arteriosclerosis and the related diseases as coronary heart disease, myocardial infarction, angina pectoris, hyperlipidemia, encephalopathy It can be used for the prevention and treatment of latent metabolic diseases such as cerebral hemorrhage, stroke, cerebral infarction, cerebral hemorrhage, vascular restenosis and obstruction as well as diabetes and its complications, obesity, and dyslipidemia.

The present invention provides an extract for preventing and treating atherosclerosis, wherein the herbal mixture is selected by extracting at least one compound from a crowd consisting of water and an organic solvent.

Herbal medicine mixture extracts according to the present invention can be obtained by extracting the herbal medicine mixture with water or an organic solvent, or a mixed solvent of water and an organic solvent, the organic solvent is lower alcohol, acetone, chloroform, methylene chloride, ether, Ethyl acetate, hexane, etc. can be illustrated. The lower alcohol may be exemplified by methanol, ethanol, propanol and butanol, with ethanol being most preferred.

When extracting the herbal mixtures using water as a solvent, 1 to 2 times, preferably 1.5 times, water of the total weight of the herbal mixture is added and 80 to 100 ° C, preferably 90 to 100 ° C. Extraction for 1 to 24 hours, preferably 3 to 10 hours at a temperature and then filtered to prepare a hydrothermal extract of the herbal mixtures according to the present invention. When extracting the herbal mixtures using an organic solvent, an organic solvent of 1 to 10 times, preferably 5 times the total weight of the herbal mixture is added, extracted at room temperature, and the filtrate obtained by filtration is concentrated under reduced pressure. It can manufacture. In the extraction methods, the extraction process may be repeated two or more times as necessary. In addition, the extracts of the herbal mixtures of the present invention include extracting the concentrated filtrate or preparing the powdered filtrate by concentrating and drying the filtrate. In addition, the drying may be any conventional method may be used but is preferably carried out by a lyophilization method.

Chinese herbal medicines used in the present invention have been used as a herbal medicine for a long time there is no problem such as toxicity and side effects. The present invention also provides health food compositions for the prevention and treatment of atherosclerosis and related diseases, characterized in that the mixed herbal extracts as an active ingredient. Herbal medicine mixture extract according to the present invention can be used as an active ingredient of the health food composition for the prevention and treatment of atherosclerosis and related diseases do not have problems such as side effects or toxicity even when administered to rats. When the extracts according to the present invention are used as food or beverage additives, the extracts may be added as is or used together with other food or food ingredients, and may be appropriately used according to conventional methods. Generally, in the preparation of food or beverages, the herbal extracts of the present invention are added in an amount of 0.0001 to 50% by weight, preferably 0.001 to 20% by weight, based on the total raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. . In addition, since the active ingredient has no problem in terms of safety, it can be taken for a long time for health and hygiene purposes or for health control purposes.

There is no particular limitation on the kind of food. The preferred health food composition according to the present invention can be formulated and administered according to a conventional method, and the hot water extract can be powdered by extracting it with water, or concentrated to extract or dried, and then dried by putting a carrier in the hot water extract. It may be encapsulated or formulated in the form of tablets, granules, beverages, powders and the like. Examples of the food to which the substance may be added include juice, juice, jelly, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream including ice cream, various soups, Drinks, pouches, ampoules, teas, drinks and vitamin complexes, and the like, and includes all of the health food in the usual sense.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at 0.001 to 300 mg / kg, preferably 0.001 to 100 mg / kg per day. Administration may be administered once a day or may be divided several times.

Hereinafter, the present invention will be described in more detail based on the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Extracts for Preventing and Treating Arteriosclerosis and Related Diseases According to the Present Invention

12 g of Baekchul, 6 g of Cheonma, 6 g of Dermis, 9 g of Bokryeong, 9 g of Sansa, 9 g of Happiness were washed well, and then put into an extraction container and 500 ml of water was added to extract hot water at 100 ° C. for 3 hours. The resulting solution was cooled at room temperature and then filtered through a filter paper. The extract was concentrated under reduced pressure at 40 ° C. or lower using a vacuum rotary evaporator and lyophilized to obtain a mixed herbal extract according to the present invention in powder form (yield: 15 w / v%). The herbal mixture extract was used in the following Experimental Example 2 and Formulation Example.

Example 2 Preparation of Extracts for Preventing and Treating Arteriosclerosis and Related Diseases

After washing 9g, Baekchul 12g, Cheonma 6g, Dermis 6g, Bokryeong 9g, Sansa 9g, Happiness 9g, 9g of rhubarb, wash well and put into an extraction container and add 500ml of water and heat it at 100 ℃ for 3 hours. Extracted. The resulting solution was cooled at room temperature and then filtered through a filter paper. The extract was concentrated under reduced pressure at 40 ° C. or lower using a vacuum rotary evaporator and lyophilized to obtain a mixed herbal extract according to the present invention in powder form (yield: 15 w / v%). The herbal mixture extract was used in the following experimental example.

Experimental Example 1 Toxicity Test of Vascular Smooth Muscle Cells According to the Concentration of the Extract Obtained in Example 2

Toxicity experiments on vascular smooth muscle cells of the extract obtained in Example 2 were observed through the MTT method. Vascular smooth muscle cells were incubated in 96 well-plates until they grew 90-100%, and then serum was depleted by grinding the existing medium with serum-free medium for 24 hours. Then, the extract obtained in Example 2 was dissolved in medium without serum and treated to cells of 96 well-plates by concentration (mg / ml). After 24 hours of treatment with the extract, MTT solution was added at a concentration of 2 mg / ml. After about 4 hours, the formalin formed on the plate was dissolved in 200 µl of DMSO, and the absorbance was measured at 540 nm with an ELISA reader. The survival rate of the cells by the sample was calculated using the following equation, and the samples showing survival rates of 70% or less with respect to the control group in each experimental group for each concentration were determined as samples showing cytotoxicity.

% Survival rate of cells = (absorbance of experimental group / absorbance of control group) × 100

The results of the experiment are shown in FIG. 1. As shown in FIG. 1, the vascular smooth muscle cells were found to be non-toxic because the cell viability was increased by about 20% in 1 mg / ml of the extract obtained in Example 2, but 5 mg / ml of the extract obtained in Example 2 was observed. It was observed that the survival rate of vascular smooth muscle cells decreased by about 50% at the concentration. Therefore, toxicity against vascular smooth muscle cells was observed at the concentration of 1 mg / ml or more of the extract obtained in Example 2.

Experimental Example 2 Effect of Inhibiting Migration of Vascular Smooth Muscle Cells According to the Concentration of the Extract Obtained in Example 1

The inhibitory effect of vascular smooth muscle cell migration was also observed by wound healing assay. Vascular smooth muscle cells were incubated until growth was about 100% in 24 well-plates, and a growth inhibitory substance, mitomycin, was administered at a concentration of 25 µg / ml to stop the proliferation of vascular smooth muscle cells. After 1 hour, using a 200 μl pipette tip, a line of constant thickness was drawn at the bottom of the well-plate, and the medium was removed and washed with PBS. Then, in the medium to which 1% FBS was added, the extract obtained in Example 1 was not administered or administered at a concentration of 1 mg / ml, and 5 ng / ml PDGF was added 30 minutes later. The distance was measured.

The experimental results are shown in Table 3 below.

PDGF (5 ng / ml) + + Extract concentration of Example 1 (mg / ml) - One Cell migration distance (%) 12 hours 95 42

Table 3 is a table showing the migrated distance in percentage. As shown in Table 1, as measured by 12 hours after PDGF alone administration, about 95% of vascular smooth muscle cell migration was observed.However, when the extract obtained in Example 1 was subjected to combat, the cell migration was about 42%. It was observed that cell migration by PDGF was inhibited.

Experimental Example 3 Inhibition of Proliferation of Vascular Smooth Muscle Cells of the Extract Obtained in Example 2

Vascular smooth muscle cells were cultured in 96 well-plates until about 50% growth, and the serum was depleted by grinding the existing medium with serum-free medium for 24 hours. Next, 100 μl of the medium in the negative control group, and 100 μl of the extract obtained in Example 2 in the medium at a concentration of 1 mg / ml in the experimental group. After 1 hour, PDGF was administered to the positive control group and the experimental group at a concentration of 5 ng / ml. Each control group and the experimental group were incubated for 12 hours to 72 hours, and after each incubation time, 50 µl of 2 mg / ml MTT solution was added, and formagen formed in each plate was dissolved in 200 µl of DMSO, followed by ELISA reader. Absorbance was measured at 540 nm.

The experimental results are shown in FIG. 2. As shown in FIG. 2, when PDGF alone was administered, vascular smooth muscle cells showed a cell survival rate of about 2 times compared to that of the negative control group, but the extract obtained in Example 2 and the PDGF combination group showed similar survival rates as the negative control group. It was.

Experimental Example 4 The effect of the extract obtained in Example 2 on the migration of vascular smooth muscle

The migration inhibitory effect of vascular smooth muscle cells was observed using a transwell coated with type I collagen. Vascular smooth muscle cells were incubated until the growth of about 100% in three plates, the existing medium was changed to a medium without serum and cultured for 24 hours to deplete the serum. 100 μl (1 × 10 ⁶ ) of vascular smooth muscle cells and the extract according to the present invention were administered to the upper chamber at 0, 0.1 and 1 mg / ml, respectively, and then 600 μl of serum was added to the lower chamber. No medium was added. After 1 hour 50 ng / ml of PDGF was added to the lower chamber and incubated at 37 ° C. After 48 hours, the upper surfaces were washed, fixed in methanol for 30 minutes, stained with Gimessa solution for 1 hour, and then the number of migrated vascular smooth muscle cells was measured.

The experimental results are shown in FIG. 3.

As shown in FIG. 3, when the PDGF alone was administered for 48 hours and the migration of vascular smooth muscle cells was represented as 100%, the extract obtained in Example 2 was administered in combination of 0.1 mg / ml and 1 mg / ml. In this case, about 80% and 40%, respectively, were observed to inhibit the migration of vascular smooth muscle cells.

Experimental Example 5 Inhibition of Migration of Vascular Smooth Muscle Cells According to the Concentration of the Extract Obtained in Example 2

The inhibitory effect of vascular smooth muscle cell migration was also observed by wound healing assay. Vascular smooth muscle cells were incubated until growth was about 100% in 24 well-plates, and a growth inhibitory substance, mitomycin, was administered at a concentration of 25 µg / ml to stop the proliferation of vascular smooth muscle cells. After 1 hour, using a 200 μl pipette tip, a line of constant thickness was drawn at the bottom of the well-plate, and the medium was removed and washed with PBS. Then, the concentration of vascular smooth muscle cells was measured at 12 hour intervals by administering the extract obtained in Example 2 to the medium to which 1% FBS was added at different concentrations, and adding 5 ng / ml PDGF after 30 minutes.

The experimental results are shown in Table 4 and FIG. 4.

PDGF (5 ng / ml) + + + + Extract concentration of Example 2 (mg / ml) - 0.1 0.5 One Cell migration distance (%) 12 hours 95 95 59 36 24 hours 100 100 76 52

4 is a photograph of vascular smooth muscle cells, and Table 4 is a table showing the migrated distance as a percentage. As shown in FIG. 4, as a result of inducing the migration of cells by PDGF administration, the migration of vascular smooth muscle cells was observed 100% at 24 hours, and when the extract obtained in Example 2 and PDGF were co-administered in Example 2 It was observed that the obtained extract suppressed the migration of vascular smooth muscle cells. In addition, as shown in Table 4, as measured by 12 hours after PDGF alone administration, about 95% of vascular smooth muscle cell migration was observed.However, when the extract obtained in Example 2 was subjected to combat, it was 59.3% and 35.5%. About 40-60% of the cell migration was observed to inhibit cell migration by PDGF. In addition, at 24 hours, 100% migration progressed in the PDGF-administered group, whereas about 50% migration of vascular smooth muscle cells was observed when the extract obtained in Example 2 was battled, and the extract inhibited cell migration by PDGF. Could be observed.

Formulation example  One. Chewing gum  Produce

0.24 to 0.64% of the extract obtained in Example 1

Gum Base 20%

Sugar 76.36-76.76%

1% of fruit flavor

Water 2%

Chewing gum was prepared using a conventional method with the above composition and content.

Formulation example  2. Manufacture of beverage

0.5 g to 1.3 g extract obtained in Example 1

522mg of honey

Chioctosanamide 5mg

Nicotinamide 10mg

Riboflavin Sodium Hydrochloride 3mg

Pyridoxine hydrochloride 2mg

Inositol 30mg

Ortic Acid 50mg

300 ml of water

With the above composition and content, beverages were prepared using conventional methods.

Formulation example  3. Manufacture of Candy

The materials used in the manufacture of the candy are as follows.

Extract obtained in Example 1

Crystalline Lactitol (Danisco Sweeteners)

water

Acesulfame K (Hoechst)

Blueberry Scent (DI 27328)

Citric Acid (Bahrat Starch Industries, Ltd)

A mixture of 20% of the extract obtained in Example 1 and 80% of crystalline lactitol (Danisco Sweeteners) was diluted with water and placed in a sauce pan. The batch was first heated on a hot plate until all material was solubilized. The batch was then transferred to a vacuum cooker and further heated. The blend formed formed a viscous mass even at relatively low temperatures. The syrup was then transferred from the cooker to the thick plate and cured until a suitable tissue for drop rolling was achieved. The cured mass was fed through a drop roller. Once imprinted, the candy had an acceptable quality.

Formulation example  4. Production of alcohol

Wash out 240 g, Cheonma 120 g, dermis 120 g, Bokryeong 180 g, Sansa 180 g, Happiness 180 g, put it in an extraction container, add 10,000 ml of water, and concentrate it for about 3 hours at 100 ° C to obtain an extract. Separately, the mixture was gradually mixed with steamed rice made from 4 kg of rice, 1 kg of traditional yeast, and 0.05 kg of jujube. The extract was poured and fermented at 10-25 ° C. for about 3 days. The fermentation broth was filtered to separate the liquid and solid portions, heated to 55-60 ° C., and purified water was added to the filtrate to adjust 10,000 ml to prepare a subject.

As described above, the extract according to the present invention significantly inhibited the proliferation of vascular smooth muscle cells increased by platelet derived growth factor (PDGF) at a concentration of 1 mg / ml, and inhibited lactose activity to atherosclerosis. And excellent effects in the prevention and treatment of related diseases.

Claims (5)

delete delete Arteriosclerosis, hyperlipidemia, coronary heart disease, containing hot water extract or ethanol extract of a mixture comprising baekchul, cheonma, dermis, bokyeong, hawthorn and euphoria, or a mixture of herbal medicines with the addition of contrary and sulfuric acid to the mixture as an active ingredient, Health foods for improving any of the diseases selected from myocardial infarction, angina pectoris, cerebral inflammation, cerebral hemorrhage, stroke, cerebral infarction, cerebral hemorrhage, vascular restenosis and obstruction, diabetes and its complications, obesity, or lipodysemia. delete delete

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