KR100742387B1 - Breeding facilities of gnotobiotic pigs for the medical and biological experiment - Google Patents

Abstract

The present invention relates to a breeding structure of sterile pig, having a facility that can fundamentally block the pollutant that may occur inside the outside and the inside of the pig, and by having a copper wire that can effectively operate, predetermined laboratory SPF pig In addition, it is effective in mass-producing QPF pigs to be used as a long-term source of biotechnology.

Sterile, Pig, Breeding, Structures, SPF, QPF

Description

Breeding facilities of gnotobiotic pigs for the medical and biological experiment}

1 is a perspective view showing a conventional pig breeding structure.

Figure 2 schematically shows a sterile pig breeding structure according to the present invention.

3 is a view showing the breeding aid zone of FIG.

4 shows the SPF zone of FIG. 2;

FIG. 5 shows the QPF zone of FIG. 2. FIG.

Figure 6 is a perspective view showing the room of the SPF zone and the QPF zone in accordance with the present invention.

7 is a cross-sectional view of FIG. 6.

8 is a block diagram showing the movement of a person according to the present invention.

Figure 9 is a block diagram showing the copper wire of the pig in accordance with the present invention.

10 is a block diagram showing the copper wire of the feed according to the present invention.

<Description of the symbols for the main parts of the drawings>

100: structure 210: breeding assistance area

310: SPF zone 410: QPF zone

510: sewage treatment area

The present invention relates to a breeding structure of sterile pigs, and more particularly, a sterile plant equipped with a facility and an operation plan to maintain a predetermined sterile state for raising pigs most similar to human anatomical characteristics for various experiments as well as a bio-organ source. A pig breeding structure.

Until now, research in medical disease models and drug safety tests, testing of therapeutic efficacy of certain therapeutic agents, in vivo metabolic processes of drugs, research on drug resistance and interactions between drugs, and development of other artificial organs, such as the development of artificial organs Therefore, most of the animals used as experimental animals are rodents such as rats, mice, and guinea pigs, and since they are different from humans in terms of anatomical physiology, the results of these experiments are directly applied to humans. It is difficult to apply in terms of the validity of the test.

In comparison, pigs have similar physiological functions and genetic structure to humans, have been living with humans for a long time, providing artificial cells or insulin, and similar to humans in terms of organ size and function in relation to organ transplantation. It has recently attracted attention in the life sciences, especially in the field of medicine, because it can produce up to 12 babies per head, thus ensuring abundant organs. However, in the case of a normal pig, the weight reaches 250-280 kg at maturity, and thus, there are many difficulties in the management of the specification and the experiment as an experimental animal.

On the other hand, genetic and microbiological standardization procedures of experimental animals are required to ensure the accuracy, uniformity, and reproducibility of the experiment. Genetic standardization of laboratory animals can be achieved through breeding techniques through special breeding programs and biotechnological techniques, and one method of microbiological standardization is the production of specific pathogen free (SPF) animals. There is a method to utilize in the experiment. Breeding of these SPF animals should be breeding and managed to prevent microbial infections such as bacteria, viruses, fungi and parasites in order to maintain a specific disease-free condition for experimental purposes.

Furthermore, in order to breed pigs as a bio-organic source for transplanting the organs of cloned pigs into the human body, breeding and management are performed to maintain a sterile state (Qualified Pathogen Free, hereinafter referred to as 'QPF') that is free of harmful pathogens. Common infectious diseases and related diseases should be blocked.

In particular, the latter case is one of the countermeasures, since the demand for organ transplants increases due to the increase of the long-term disease and degenerative diseases due to the increase of the world population and the aging society. There is much interest and research on organ transplantation technology.

As a result, it has been found that superacute and acute rejection, which have been a problem in transplanting pig organs, can be overcome by producing certain transgenic cloned pigs (eg, alpha-gal transgenic pigs). Efforts have been made to overcome by regulation or induction of immune tolerance.

Therefore, there is a need for a facility for mass production of a transgenic cloned pig for a bio-organic source and a xenotransplantation research experiment, that is, a sterile pig breeding facility having QPF conditions, but there are currently no specific technologies related thereto.

However, as shown in Figure 1, conventional pig breeding facility is simply pigs 10 consisting of a roof 13 to form the bottom surface 11 and the wall surface 12 and cover the upper with a concrete or the like is formed The work path 15 is provided in the central longitudinal direction in the inside of the pig 10, and partitions 17 are provided on both sides of the work path 15 to form a plurality of pig rooms 16. It is widely used.

Such a conventional pig breeding facility is not equipped with a separate device that can handle the waste, such as pig meal, pig urine generated during the breeding process, simply clean and disinfect periodically, so that the pig flour or pig urine is not cleaned. Until it is left untouched, it smells like odor inside the pig house, becomes a habitat for germs and pests, and it is also a major cause of polluting groundwater and rivers by leaking pig urine.

To solve these problems, various techniques for maintaining and managing clean pigs have been developed and applied, but most of them are related to improving the pigs' environment, and can only solve some problems such as odors generated from pigs. There is no device to prevent the invasion of various germs, and is still exposed to various diseases such as foot-and-mouth disease, swine cholera, infectious gastroenteritis, swine epidemic diarrhea, and Ozeski's disease.

In particular, pigs bred in a conventional breeding facility may be used for food, but are not microbiologically selected, so when used as a long-term source of humans, pathogens may be transferred from pigs to humans, and microbes exhibiting non-pathogenicity in pigs. There is also a possibility that these humans may show pathogenicity, and there are many problems that are unsuitable for use in biological and medical experiments.

The present invention has been made to solve this problem, a sterile pig breeding structure equipped with a facility and operation plan to maintain a predetermined aseptic condition for raising pigs most similar to human anatomical characteristics for various experiments and bio-organ sources. The purpose is to provide a description of the technology.

The present invention provides a pig breeding zone, pig breeding subsidiary zone, entry and exit zones, sewage treatment zone, and ventilation and other facilities of pigs and other facilities, the pig breeding zone is a plurality of It is divided into a pig room, and the entrance and exit of each pig room is provided with an entire room, and the breeding auxiliary area is composed of an indoor preparation room and a laboratory adjacent to the pig breeding area, and the entry and exit area is for a person to enter and exit the breeding auxiliary area. And a pig inlet for introducing the pigs and other objects into the breeding aid zone, and a pig outlet for exporting the pigs and other goods from the breeding aid zone or the pig breeding zone. Treating the dirt from the pig breeding area and the breeding auxiliary area by the pig Adjacent to the meat area is installed underground or outside of the pig breeding structure, the ventilation and other facilities are a ventilation facility for maintaining a predetermined cleanliness, predetermined sterilized drinking water and the pig breeding area and It includes a water supply facility for supplying a predetermined washing water to the breeding auxiliary area, and an electrical facility for proper temperature control of the pig breeding area, each piglet in the pig breeding area is separated from the predetermined specific pathogens such as floor, wall, It is characterized in that the ceiling surface is sealed.

Preferred embodiments for implementing the present invention as described above are as follows, each of which is another feature of the present invention.

First, the pig breeding zone may be divided into the SPF zone and the QPF zone adjacent to the breeding subsidiary zone,

The SPF zone is again connected to the SPF corridor with the whole room adjacent to the breeding aid zone; The SPF corridor between the SPF piglets, SPF breeding pig room, SPF pregnant pig room, SPF delivery pig room can be partitioned on both sides, and further includes an air piglet in which SPF sows implanted with a given fertilized egg are reared until delivery. Can be partitioned,

A QPF corridor connected to the QPF area with an entire room adjacent to the breeding auxiliary area; The QPF corridor between the two can be divided into QPF piglets, QPF rearing piglet, QPF pregnant piglet, QPF delivery piglet, and further divided into a QPF preparation room for breeding QPF pigs in the QPF area. have.

The indoor preparation room of the breeding auxiliary area includes a warehouse for storing consumables and feed brought into the pig inlet, and an internal preparation room for disinfection and anesthesia of the pig body. A first operating room for implanting fertilized eggs, a second operating room for caesarean section, an isolator room adjacent to the second operating room, an autopsy cleaning room for cleaning sows for surgery, an autopsy of laboratory pigs, and a dead body; It may be configured as a freezer compartment for freezing the dead body.

The entrance and exit of the person includes a shower room for men and a shower room separated from the entrance; The shower room may be composed of a male shower restroom and a female shower restroom and the breeding auxiliary area, and further preferably, the entire chamber is equipped with an infrared sterilizer and an air shower.

On the other hand, it is preferable that the entire room where the breeding auxiliary area and the QPF corridor are adjacent is equipped with a personal decontamination system (PDS).

In addition, it is preferable that all chambers at the entrance of each pig room of the pig breeding area have a structure in which both doors with airtightness are not opened at the same time, and the air pressure of the chambers is set higher than the surroundings.

And, the pig inlet is preferably equipped with a disinfection room that can disinfect any of the objects brought in from the outside,

The pig outlet is a QPF experimental pig outlet connected to the QPF corridor and the front chamber; An SPF test outlet outlet connected to the SPF corridor and the front room; It may be configured as a sow outlet for taking out the sow stored in the freezer.

In addition, it is preferable that the pig room is composed of a plurality of cages, the partition of the cage is composed of two stages, and the bottom of the cage is composed of a plurality of rods.

In addition, the sewage treatment zone is a dust collecting chamber for collecting the sewage in a specific direction is the bottom of the pig breeding area is gradient; A drain pipe connected to the dust chamber; It may be composed of a waste water treatment chamber for purifying the sewage flowing into the drain pipe.

In addition, the ventilation facility is the SPF zone and the breeding assistance zone is based on class 10,000, the QPF zone is preferably ventilated on the basis of class 1,000,

The air-conditioning duct of the ventilation system adopts a Top Down Air Flow method, so that the air flow flows from the low pollution area to the high pollution area,

In particular, it is preferable that the ventilation system is an all-out air system, wherein the SPF section uses a HEPA filter at the inlet and a carbon filter at the outlet, and the QPF section uses a ULPA filter at the inlet and a HEPA filter at the outlet. Do.

In addition, the water supply facility of the SPF zone uses R / O water or chlorine sterilized water as the negative water, and the chlorine sterilized water is used as the wash water, and the water supply facility of the QPF zone is R / O water and chlorine. It is preferable to use the sterilized water as drinking water and washing water,

The electrical facilities of the pig breeding area is installed in each pig room with a halogen lamp to light 12 hours a day with illumination of 150 ~ 300 lux, the temperature is 21 to 25 ℃, humidity is preferably maintained 40 to 60%. .

And, the bottom of each piglet is injected into the concrete to be gradient in a specific direction, and the walls of each piglet is made of impermeable brick or epoxy paint grouted with epoxy, and the cloth of each piglet The front face is preferably made of vinyl cloth having waterproofness and impermeability.

Further, it is preferable to operate the sterile pig breeding structure by displaying the entrance and exit movement lines of the person in the human entrance and exit, the breeding assistance zone and the pig breeding zone, wherein the copper movement of the person indicated in the pig breeding zone is the SPF zone. It is desirable to distinguish the and QPF areas so that they do not cross each other.

In addition, the pig inlet, pig outlet, breeding assistance zone and pig breeding zone is preferably marked pig pigeon,

To remove SPF test pigs, pig inlet (sterilization room)-> autopsy cleaning room (washing)-> internal preparation room (body disinfection, anesthesia)-> second operating room (cesarean section)-> isolator room (pigment protection) -> SPF whole room-> SPF piglet-> SPF nursing pig room-> SPF pregnant pig room-> SPF delivery pig room-> SPF whole room-> SPF experimental pig outlet, it is preferable to mark, here to remove sows after caesarean section It is more preferable to further display the second operating room (caesarean section)-> autopsy cleaning room (sow washing)-> freezing room (sow storage)-> sow outlet.

To remove the QPF test pigs, the pig copper inlet (sterilization room)-> autopsy cleaning room (washing)-> internal preparation room (body disinfection, anesthesia)-> second operating room (cesarean section)-> isolator room (piglet protection) -> SPF all rooms-> SPF piglet-> SPF nursing pig room-> SPF pregnant pig room-> SPF delivery pig room-> SPF all rooms-> autopsy cleaning room (washing)-> internal preparation room (body disinfection, anesthesia)-> 1st Operating room (fertilized egg transplant)-> SPF room-> waiting pig room (fertilized egg)-> SPF room-> autopsy room (washing pregnant)-> internal preparation room (body disinfection, anesthesia)-> second operating room (cesarean section)- > Isolator room (Swine protection)-> QPF all rooms (PDS, PR)-> QPF corridor-> QPF pig room-> QPF preparation room (in-house propagation)->-QPF room-> QPF experimental pig outlet In this case, it is more preferable to mark the second operating room (cesarean section)-> autopsy washing room (sow washing)-> freezing room (sow storage)-> sow outlet for the sow removal after cesarean section.

Hereinafter, the embodiment will be described in detail with reference to the accompanying drawings.

First, as shown in Figure 2, schematically showing the aseptic pig breeding structure 100 according to the present invention, there is a pig breeding zone (310, 410) adjacent to the breeding auxiliary area 210 as a reference.

The breeding auxiliary area 210, as shown in Figure 3, the entrance and exit door 211, the man shower return room (212a), the woman shower return room (212b), an infrared sterilizer and an air shower room for the person to enter and exit 213, an internal preparation room 214 for disinfection and anesthesia of the pig body, a first operating room 215 for implanting fertilized eggs into SPF pigs, a second operating room 216 for caesarean sows, and in the second operating room Isolator room for protecting piglets born by cesarean section (217), pig inlet (218) for importing pigs and other items, disinfection room (219) for disinfecting pigs, etc. Warehouse 220, which stores goods and feed, etc., washing of sows for surgery, autopsy of laboratory pigs, autopsy washing room 221 for arranging carcasses, freezer 222 for freezing and storing the carcasses, and the freezer Sows that carry out sows stored in It consists of the outlet 223, and the mechanical / electrical room 110 for controlling the mechanical / electrical facilities of the sterile pig breeding structure 100.

In addition, the pig breeding area adjacent to the breeding auxiliary area 210, as shown in Figure 2, the SPF section 310 on one side, the QPF section 410 on the other side to isolate each other to meet the purpose Raise money. Of course, depending on the purpose of the facility, the pig breeding area may be a SPF area only or a QPF area only.

The SPF section 310, as shown in Figure 4, connected to the breeding subsidiary area 210, where the person or pigs, etc., the entrance room 311, SPF corridor 312, equipped with an infrared sterilizer and an air shower, SPF piglet (313), SPF breeding piglet (314), SPF pregnant piglet (315), SPF delivery piglet (316), atmospheric piglet (317), male and fertilized eggs implanted until the birth sow so that cloned fertilized eggs SPF take out pigs 318, 314 ', 314', 315 ', 316', 317 ', 318', which are bred to the outside carrying air for breeding sows, etc. The discharge outlet 319, and the front chamber 319 'of the SPF experimental pig outlet.

In addition, an air conditioner 320 and a large ventilator 321 are provided as a ventilation facility of the SPF zone 310, and a predetermined sewage treatment zone 510 is also installed.

In particular, the ventilation system of the SPF zone is required to be installed and operated to meet the standard of class 10,000. More specifically, it is preferable to use a HEPA filter for the inlet of the air conditioner 320, and a carbon filter for the outlet.

In addition, the sewage treatment zone 510 is a dust collecting chamber for collecting sewage in a specific direction by the bottom of the SPF zone, a drain pipe associated with the dust collecting chamber, and a wastewater treatment chamber for purifying sewage flowing into the drain pipe. Can be configured.

Similarly, the QPF area 410 is connected to the breeding assistance area 210, as shown in FIG. 5, where all rooms 411, QPF corridors 412, and QPF piglets are equipped with a PDS facility where a human or a pig is introduced. (413), QPF rearing piglet (414), QPF pregnant piglet (415), QPF birthing piglet (416), QPF preparation room (417) for breeding QPF pigs in QPF itself, males and females that are not crossbreeding, etc. Experimental pig room for breeding QPF for breeding as an external carry-out atmosphere for experimental and long-term supply, all rooms (413 ', 414', 415 ', 416', 418 '), QPF laboratory pig outlet (419) ), And the front chamber 419 'of the QPF experimental pig outlet.

In addition, the QPF zone 410 is also equipped with ventilation facilities 420, 421 and sewage treatment zone 510, the ventilation facilities of the QPF zone is required to be installed and operated to meet the class 1,000 standards, The air conditioner 420 of the ventilation system preferably uses a ULPA filter for the inlet and a HEPA filter for the outlet.

Next, each piglet of the SPF zone 310 and the QPF zone 410 may have the same structure. The structure of the piglet is described with reference to FIG. 6, which shows one of the piglets in detail 313. When described as follows.

First, there is a front door 313 'having a double door in the SPF corridor 312, and a plurality of cages 332 are formed on both sides of the corridor 331. The bottom 333 of the cage is composed of a plurality of rods, in addition to the air conditioner (air conditioning duct, 334), water supply facilities (335a, 335b), feed tank (336), lighting equipment for supplying air to the room and pig room 337 is provided and there is a dust collecting chamber 512 at the bottom.

Next, the structural features, ventilation, and sewage treatment methods of the pig room will be described in more detail with reference to FIG. 7 showing one cross section of FIG. 6 as follows.

The front chamber 313 'is a double door with airtightness, and is always maintained at a higher air pressure than the SPF corridor 312 by the air pressure supplied by the air conditioner 334 installed on both sides of the upper part of the pig compartment. By not using it, it is possible to prevent the pollutant in the SPF corridor 312 from entering the room when entering or exiting the room.

In addition, the cage is divided into a plurality by the height adjustable partition, both sides of the partition is provided with a water dispenser 335b and the feeding container 338 symmetrically spaced when the pigs drink water, the water falls into the feeding container The negative water supplied to the water supply is supplied to one water supply pipe 335a installed at the bottom of the air conditioner 334.

At this time, the negative water supply pipe 335a supplied to the pig room may be used as one water supply pipe if the SPF zone 310 is used, but it should not be used together in the QPF zone 410. This is sufficient if the negative water in the SPF zone 310 uses R / O water or chlorine sterilized water, but the negative water in the QPF zone 410 should be used such as R / O water and chlorine sterilized water. This is because the water used is different.

The bottom of the cage is composed of a plurality of rods at intervals in which the pig's legs do not fall out, so that the manure of the pig is discharged to the lower dust collecting chamber 512 through the space between the rods 333 so as not to accumulate on the bottom of the cage do.

On the other hand, the manure of the pig collected in the dust collecting chamber 512 is moved to the sewage treatment area 510 by the bottom surface 514 of the dust collecting chamber gradientd at a predetermined angle. That is, the sewage collected in a specific direction by the bottom surface 514 of the grated dust chamber is moved to a wastewater treatment chamber (not shown) through a drain pipe (not shown) connected to the dust chamber 512 to a predetermined discharge water. Is purified.

In addition, the ventilation facility of the pig room receives a predetermined purified air by the air conditioning duct 334 installed on both sides of the upper piglet, and a large discharge fan (ventilator; 321) installed in connection with the dust collecting chamber 512 outside the pig house. By forcibly forming the air flow of the piglet in the Top Down Air Flow method, the air flows from the low polluted area to the high polluted area so that the contaminants of neighboring pigs are not transmitted, and the It also serves to help move more smoothly along the graded bottom surface 514. Here, the air purification filter required for the ventilation conditions and the air conditioning duct 334 is as described above.

The upper part of the dust collecting chamber 512 below the front chamber 313 ′ may be used to remove impurities such as manure remaining on the bottom surface of the dust collecting chamber with a high-pressure washing water by a predetermined piping facility (not shown).

In addition, the electrical facility of Donbang is properly installed with a lamp (337) on the ceiling, and lights up about 12 hours a day with illumination of 150 to 300 lux (from 7 am to 7 pm), and the temperature is 21 to 25 ° C. and humidity. It is desirable to maintain 40 to 60%. In order to adjust the temperature and humidity of the pig room, the lamp 337 may be installed by lowering the lamp 337 to an appropriate length from a ceiling with a halogen lamp, and a switch for turning on the lamp 337 and adjusting the illuminance may be provided in the front room 313 '. desirable.

In addition, the pig room is provided with a separate gazebo (not shown) on one side wall of the pig room so that the inside of the pig room can be observed in the breeding auxiliary area 210, and also equipped with a sunshade that can block the gazebo. Can be.

In addition, the floor (dust collection floor), walls, and ceiling surfaces of the pig room should be completely sealed, so that bacteria, etc. invade the pig room from other areas or the outside. To this end, the dust collecting chamber floor 514 is injected into the concrete to be gradient in a specific direction, and the walls of the pig room use impermeable bricks or epoxy blocks grouted with epoxy, and the ceiling surface of the pig room. It is desirable to seal the vinyl cloth with silver waterproof and impermeable. That is, the floor, the wall, and the ceiling may be made of a material resistant to water, chemical resistance, abrasion resistance, impact resistance, heat resistance, and the like.

In particular, certain pathogens that are blocked in each pig house with such facilities and proper disinfection

Brucella suis , Leptospira species , Listeria monocytogenes , Mycobacterium bovis , Mycobacterium tuberculosis, Mycobacterium avium -intracellulare complex , Mycobacterium hyopneumoniae , Salmonella enterica subsp. typhi , Salmonella enterica serovar typhimurium , Salmonella enterica subsp. bacteria consisting of cholerasuis or Shigella species;

Ascaris suum , Echinococcus sp. , Cryptosporidium parvum , Strongyloides ransomi, Toxoplasma gondi , Trichinella spiralis , Neospora a parasite consisting of a species or Isospora species;

Syndrome virus Candida species, Aspergillus species, Cryptococcus neoformans or Histoplasma fungi consisting of capsulatum ;

It is preferred to include a virus consisting of Porcine adenovirus, Porcine hepatitis E, Encephalomyocarditis virus, Influenza virus, Porcine cytomegalovirus, Porcine a-herpesvirus, Porcine reproductive and respiratory virus, Porcine circovirus, Porcine parvovirus, Rabis virus or Pseudorabies virus.

In addition, the mechanical / electrical room 110 is equipped with a central control system for controlling the mechanical / electrical facilities related to the structure of the present invention, in particular, including an air conditioner, a fan, water supply and drainage, sanitation, electricity, fire fighting and disaster prevention. . Furthermore, it is desirable to have an emergency power generation facility. In addition, it is preferable to block the noise, vibration, and electromagnetic waves generated in the mechanical / electrical chamber 110 to prevent the pigs from being transferred to the branch breeding area such that the pigs in the pig breeding area are not subjected to stress.

Next will be described the operation (function) between the components of the breeding assistance zone 210, SPF pig breeding zone 310 and QPF pig breeding zone 410.

First, the male / female shower return room (212a, 212b) of the breeding auxiliary area 210 to prevent the invasion of germs from the outside of the person, and after taking off the usual clothes showered with water and then sanitary clothes After changing the sterilization and air shower in the room (213) equipped with an infrared sterilizer and an air shower is entered.

On the other hand, the disinfection room 219 is for disinfecting pigs, feeds, other goods, and equipment used in the inside, sterilization apparatus (for example, autoclave, EO gas, etc.) to carry and sterilize feeds A sterilizer) and a pig-carrying cage sterilizer. Also, all items except humans must be partitioned so that they can pass through and enter the interior (cleaning area).

In this way, in the male / female shower restroom 212a, 212b and the disinfection chamber 219, all germs which can penetrate from the outside are provided adjacent to the entrance 211 and the entrance 218, respectively. It will shut off automatically.

Then, to enter the SPF pig breeding zone 310, the room passes through the front room 311 equipped with an infrared sterilizer and an air shower again, and to enter the QPF pig breeding zone 410, the whole room having facilities such as a reinforced PDS ( 411).

Here, the PDS (Personal Decontamination System) is a mechanism that disinfects the entire outer surface of a person with a disinfectant, which is necessary for aseptic identification of the personnel entering the QPF area.

However, the front rooms 311 and 411 may function as a PR (Pass Room) in the case of pigs and goods, where secondary disinfection is performed by formalin, gas, spraying, and the like.

The next pass through the double doors (313 ', 314', 315 ', 316', 317 ', 318', 413 ', 414', 415 ', 416' and 418 ') The function of the front room is to physically block the contaminants of the SPF corridor or the QPF corridor from entering the room as mentioned above.

In addition, the SPF experimental pig outlet 319 and the QPF experimental pig outlet 419 are also placed in the front chamber (319 ', 419'), respectively, to physically block the external contaminant from entering inside when the experimental pigs are taken out. At this time, the air pressure of the front chambers 319 'and 419' is required to be sufficiently higher than the external atmospheric pressure.

By doing so, each piglet of the SPF zone 310 and the QPF zone 410 is sufficiently blocked from external pollutants to maintain a state in which specific pathogens are removed.

On the other hand, in order to remove the pollutants caused by pigs raised in each pig room, as described above, the water supply and feeding facilities are spaced apart, and a predetermined manure processing device (the bottom of each pig house has a plurality of rods, and While falling into the space between the inclined and the bottom of the dust chamber is inclined, while manure treatment with a high-pressure washing water by a predetermined piping facility) and the ventilation facility (Top Down Air Flow method) of each zone is essential.

Since the ventilation facilities are different in cleanliness of each zone, it is preferable to install a separate air conditioner and a fan, and their operation must be separately operated so that predetermined cleanliness of each zone is satisfied (SPF zone: class 10,000, QPF zone: class). 1,000).

However, the breeding assistance zone 210 may have the same ventilation facility as the SPF zone 310.

In addition, in order to prevent contamination due to the water supply or washing water, as previously mentioned, a predetermined sterilized one is used, and the water supply pipe is installed by separating the SPF zone and the QPF zone.

In addition, each of the internal compartments of the breeding assistance zone 210, the SPF pig breeding zone 310, and the QPF pig breeding zone 410 may vary according to the purpose, and is not limited to the above-described embodiment.

Finally, in order to effectively use the sterile pig breeding structure according to the present invention and maintain a predetermined sterile state, copper wires representing the movement paths of humans, pigs, and other articles are displayed in each zone.

First, the movement of a person may vary depending on the worker and the visitor, but once all enter the breeding assistance area 210 according to the present invention, as shown in FIG. 8, the shower return rooms 212a and 212b connected to the entrance 211. After taking a shower in a predetermined clothes and then in the front room 213 should be infrared sterilization and air shower.

After that, only the relevant workers once again sterilize the infrared sterilization and air shower in the SPF room 311 and enters the SPF money room (313, etc.) via the SPF corridor (312). Of course, as mentioned above, before entering the SPF room, each room will go through all rooms (131 ', etc.). Likewise, the QPF area will be sterilized thoroughly by PDS, etc. in the entire QPF room 411, and enter the QPF money room through the QPF corridor. In this case, in order to prevent mutual infection with the SPF region, it is preferable to mark them so as not to cross each other.

The visitor observes the pig room through the observation window (not shown) in the internal preparation room 214 of the breeding assistance area 210.

When you come out, you can come out in the reverse order. You do not need to disinfect and shower in each room.

Next, the pig's copper wire can be largely divided into the SPF experimental pigs and the QPF experimental pigs. In the former case, as shown in FIG. 9, external pregnant pigs are brought into the pig inlet 218 (this is the first time). Necessary only when operating the facility), imported pregnant pigs are inspected and disinfected in the disinfection room 219, then washed in the autopsy washing room 221, body disinfection and anesthesia in the internal preparation room 214, 2 cesarean section in the operating room 216 to obtain the piglets, the sows are transferred to the autopsy cleaning room 221, washed, and then stored in the freezer compartment 222 and taken out through the sow outlet 223, above The piglets obtained are placed in an incubator of the isolator chamber 217 and passed through the SPF front chamber 311 to be reared through the SPF piglets / growing piglets 313 and 314, and are transferred to the SPF pregnant piglet 315 when pregnant. When it's time to go to bed Childbirth is induced, and partly male sows and males are relocated to SPF pregnant piglets (315, 314) and back to SPF pregnant piglets (315). It is carried out to the outside through the SPF experimental money outlet 319. At this time, the male piglets are moved from the SPF delivery pig room 316 to the experimental pig room for transporting SPF 318 and waited for a while, and then transported to the outside through the SPF experimental pig outlet 319 via the SPF chamber 319 '. It may be.

In the case of QPF experimental pigs, as shown in Figure 9, when the female piglets obtained in the SPF delivery piglet 316 about 6 days of weaning, the sows are passed through the SPF chamber (PR; 311) and the autopsy washing room ( 221, then disinfect and anesthetize the body in the internal preparation room 214, implant a predetermined cloned egg in the first operating room 215, and pass through the SPF chamber 311 if implantation is found. Breeding in the waiting room until 317, and when the delivery time is again passed through the SPF room 311 to wash the pregnant pigs in the autopsy cleaning room 221, and then the necessary disinfection and anesthesia in the internal preparation room 214 and the second The piglets are obtained by caesarean section in the operating room 216, the sows are transferred to the autopsy cleaning room 221, washed, and then stored in the freezer compartment 222 and taken out through the sow outlet 223, the piglets obtained above Incubator in isolator room 217 In the QPF pig room (413 to 418) and breeding in each QPF pig room (413) through the QPF corridor 411 through the QPF corridor through the QPF corridor (natural delivery), etc. After waiting, if necessary, the tank is taken out through the QPF test chamber 419 'to the outside through the QPF test money outlet 419.

If the implantation is not implanted after transplanting the cloned fertilized egg in the first operating room 215 through the QPF front chamber 411 and SPF front chamber 311 is transferred to the experimental pig room for transporting SPF 318 and later SPF chamber (319 '). Through SPF experiment money outlet 319 is carried out for SPF experiment.

Then, the feed line is fed to the pig inlet 218 as shown in Figure 10, and after a predetermined disinfection procedure in the disinfection room 219, and then temporarily stored in the internal warehouse 220, the SPF zone for SPF Through the front room 311 is stored in the SPF warehouse and supplied to each SPF pig room (313 to 318) if necessary, and for the QPF area through the QPF front room 411, through the QPF corridor, to the QPF preparation room 417 Stored and supplied to each QPF piglet 413-418 as needed. Here, the SPF warehouse can be used to replace one of the SPF pig room.

Other article coppers may also be displayed in accordance with the feed copper.

As described above, by varying the copper wires of the imported goods such as humans, pigs and feeds, and the copper wires for disposal of dirt and carcasses, cross-infection is prevented from each other.

In particular, when two or more need to move along the same copper wire, it is preferable to prevent the cross infection by varying the moving time.

Although a preferred embodiment of the present invention has been described in detail above, those skilled in the art to which the present invention pertains have various forms of the present invention within the technical spirit of the present invention described in the appended claims. It can be carried out by modifying or changing, the description thereof is omitted.

Sterile pig breeding structure according to the present invention is equipped with a facility that can fundamentally block the pollutant generated from the outside and the inside of the pig, and equipped with a copper wire that can effectively operate, as well as a predetermined experimental SPF pig bio-organ It is effective in mass production of QPF pigs to be used as a source.

Claims (29)

In pig breeding zones, breeding aid zones, entry and exit areas for humans and other pigs, sewage treatment areas, and pig breeding structures with ventilation and other facilities, The pig breeding area is divided into a plurality of pig rooms, the entrance of each pig room is equipped with a full room, The breeding auxiliary area is composed of an indoor preparation room and a laboratory adjacent to the pig breeding area, The entry / exit zone may include a person entrance / exit for a person to enter and exit the kennel, a pig inlet for bringing pigs or other goods into the kennel, and a pig or other article for export from the kennel or the kennel. It is divided into a pig outlet. The sewage treatment zone is disposed in the basement or outside of the pig breeding structure adjacent to the pig breeding zone to treat wastes from the pig breeding zone and the breeding auxiliary zone. The ventilation and other facilities include a ventilation facility for maintaining a predetermined cleanliness, a water supply facility for supplying predetermined sterilized drinking water to the pig breeding area and a predetermined washing water to the pig breeding area and the breeding auxiliary area, and the pig. Includes electrical facilities for proper temperature control of the breeding area, Swine breeding structure, characterized in that the bottom, wall, ceiling surface is sealed so that each pig room of the pig breeding area is isolated from a predetermined specific pathogen. The method of claim 1, The pig breeding zone is a sterilized pig breeding structure, characterized in that the breeding subsidiary area adjacent to each other divided into SPF zone and QPF zone. The method of claim 2, The SPF zone is An SPF corridor connected with the entire room adjacent to the breeding assistance area; Aseptic pig breeding structure, characterized in that partitioned between the SPF piglets, SPF breeding pig room, SPF pregnant pig room, SPF delivery pig room between the SPF corridor. The method of claim 3, wherein The SPF section is a sterile pig breeding structure, characterized in that the compartment further comprises a spawning SPF sows implanted with a given fertilized egg until breeding. The method of claim 2, The QPF zone is A QPF corridor connected with the entire room adjacent to the breeding assistance area; Aseptic pig breeding structure, characterized in that divided into the QPF piglets, QPF rearing piglet, QPF pregnant piglet, QPF delivery piglet on both sides of the QPF corridor. The method of claim 5, The QPF zone is aseptic pig breeding structure, characterized in that the compartment further comprises a QPF preparation chamber capable of breeding the QPF pig in itself. The method according to any one of claims 1 to 6, The indoor preparation room of the breeding auxiliary area includes a warehouse for storing consumables and feeds brought into the pig inlet, and an internal preparation room for disinfection and anesthesia of the pig body. The laboratory of the breeding auxiliary area includes a first operating room for implanting a predetermined fertilized egg, a second operating room for caesarean section, an isolator room adjacent to the second operating room, washing of sows for surgery, an autopsy of an experimental pig, A sterile pig breeding structure comprising an autopsy cleaning room for organizing dead bodies, and a freezer compartment for freezing and storing the dead bodies. The method of claim 7, wherein The entrance and exit of the person is separated from the entrance door shower room and woman shower recovery room; Sterile pig breeding structure, characterized in that consisting of the entire room is installed between the male shower room and the female shower room and the breeding auxiliary area. The method of claim 8, The pig inlet is a sterile pig breeding structure, characterized in that equipped with a disinfection room to disinfect any of the objects brought in from the outside. The method of claim 9, The pig outlet is A QPF experimental money outlet connected to the QPF corridor and the front chamber; An SPF test outlet outlet connected to the SPF corridor and the front room; Sterile pig breeding structure, characterized in that consisting of the sow outlet for taking out the sow stored in the freezer. The method of claim 10, The whole room is installed between the male shower room and the female shower room and the breeding auxiliary area is a sterilized pig breeding structure, characterized in that the infrared sterilizer and the air shower. The method of claim 11, Aseptic pig breeding structure, characterized in that the entire room in the vicinity of the breeding auxiliary area and the QPF corridor is provided with a PDS. The method of claim 12, Sterile pig breeding structure, characterized in that the entrance and exit movement line of the person is displayed in the human entrance and exit, breeding assistance area and pig breeding area. The method of claim 13, Aseptic pig breeding structure, characterized in that the copper wire of the person displayed in the pig breeding zone is displayed not to cross each other by separating the SPF zone and the QPF zone. The method of claim 12, Aseptic pig breeding structure, characterized in that the pig copper line is marked in the pig inlet, pig outlet, breeding assistance zone and pig breeding zone. The method of claim 15, The pig copper wire is a pig inlet (sterilization room)-> autopsy cleaning room (washing)-> internal preparation room (body disinfection, anesthesia)-> second operating room (cesarean section)-> isolator room (piglet protection) -> SPF whole room-> SPF piglet-> SPF nursing pig room-> SPF pregnant pig room-> SPF delivery pig room-> SPF whole room-> SPF experimental pig outgoing structure, characterized in that the outlet marked. The method of claim 16, The pig wire is a sterile pig breeding structure, characterized in that the second operating room (cesarean section)-> autopsy washing room (sow washing)-> freezing room (sow sow)-> sow outlet is further displayed for the removal of sows after cesarean section . The method of claim 15, The pig copper wire is used to carry out QPF test pigs (sterilization room)-> autopsy cleaning room (washing)-> internal preparation room (body disinfection, anesthesia)-> second operating room (cesarean section)-> isolator room (pigment protection) -> SPF all rooms-> SPF piglet-> SPF nursing pig room-> SPF pregnant pig room-> SPF delivery pig room-> SPF all rooms-> autopsy cleaning room (washing)-> internal preparation room (body disinfection, anesthesia)-> 1st Operating room (fertilized egg transplant)-> SPF room-> waiting pig room (fertilized egg)-> SPF room-> autopsy room (washing pregnant)-> internal preparation room (body disinfection, anesthesia)-> second operating room (cesarean section)- > Isolator room (Swine protection)-> QPF all rooms (PDS, PR)-> QPF corridor-> QPF pig room-> QPF preparation room (in-house breeding)->-QPF room-> QPF experimental pig outlet Aseptic pig breeding structure. The method of claim 18, The pig wire is a sterile pig breeding structure, characterized in that the second operating room (cesarean section)-> autopsy washing room (sow washing)-> freezing room (sow sow)-> sow outlet is further displayed for the removal of sows after cesarean section . The method of claim 12, The money room is composed of a plurality of cages, The partition of the cage consists of two stages, Sterile pig breeding structure, characterized in that the bottom of the cage consists of a plurality of rods. The method of claim 20, The sewage treatment zone is A dust collecting chamber configured to slope the bottom of the pig breeding area to collect sewage in a specific direction; A drain pipe connected to the dust chamber; Sterile pig breeding structure, characterized in that consisting of a waste water treatment chamber for purifying the sewage flowing into the drain pipe. The method of claim 21, The ventilation facility is a sterile pig breeding structure, characterized in that the SPF zone and the breeding assistance zone is based on class 10,000, the QPF zone is ventilated on the basis of class 1,000. The method of claim 22, The air conditioning duct of the ventilation facility adopts a Top Down Air Flow method, aseptic pig breeding structure, characterized in that the air flow from the low pollution area to the high pollution area. The method of claim 23, All rooms at the entrance of each pig room in the pig breeding area are constructed so that both doors of confidentiality cannot be opened at the same time. Sterile pig breeding structure, characterized in that the air pressure of the front room is set higher than the surrounding. The method of claim 23, The ventilation system is an external air system, The ventilation system of the SPF zone uses a HEPA filter at the inlet and a carbon filter at the outlet, respectively. The ventilation facility of the QPF zone is a sterile pig breeding structure, characterized in that using the ULPA filter in the inlet, and the HEPA filter in the outlet respectively. The method of claim 23, The water supply facility of the SPF zone uses R / O water or chlorinated water as negative water, chlorinated water as washing water, The water supply facility of the QPF zone is a sterilized pig breeding structure, characterized in that the R / O water and chlorine sterilized water is used as drinking water and washing water. The method of claim 23, The electrical facilities of the pig breeding area is installed in each pig room with a halogen lamp to light for 12 hours a day with illumination of 150 ~ 300 lux, temperature is 21 ~ 25 ℃, humidity is maintained to 40 to 60% Aseptic pig breeding structure. The method of claim 23, The bottom of each pig room is injected into the concrete to be gradient in a specific direction, The wall of each pig room is made of an impermeable brick or epoxy block grouted with epoxy, The ceiling surface of each pig room is a sterile pig breeding structure, characterized in that the vinyl cloth with waterproof and impermeable. The method of claim 28, Certain specific pathogens blocked in each pig room Brucella suis , Leptospira species , Listeria monocytogenes , Mycobacterium bovis , Mycobacterium tuberculosis, Mycobacterium avium -intracellulare complex , Mycobacterium hyopneumoniae , Salmonella enterica subsp. typhi , Salmonella enterica serovar typhimurium , Salmonella enterica subsp. bacteria consisting of cholerasuis or Shigella species; Ascaris suum , Echinococcus sp. , Cryptosporidium parvum , Strongyloides ransomi, Toxoplasma gondi , Trichinella spiralis , Neospora a parasite consisting of a species or Isospora species; Syndrome virus Candida species, Aspergillus species, Cryptococcus neoformans or Histoplasma fungi consisting of capsulatum ; Aseptic piglet characterized by comprising a virus consisting of Porcine adenovirus, Porcine hepatitis E, Encephalomyocarditis virus, Influenza virus, Porcine cytomegalovirus, Porcine a-herpesvirus, Porcine reproductive and respiratory virus, Porcine circovirus, Porcine parvovirus, Rabis virus or Pseudorabies virus Breeding structures.

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