KR101878847B1 - Artificial nursing method for specific pathogen free pig - Google Patents

Abstract

The present invention provides a method of feeding a mixed milk in which water is appropriately blended with water in place of milk of a sterile surrogate mother for two weeks after birth according to a predetermined schedule, The present invention provides an artificial feeding method of an SPF pig capable of effectively mass-producing the SPF pig.

Description

[0001] ARTIFICIAL NURSING METHOD FOR SPECIFIC PATHOGEN FREE PIG [0002]

The present invention relates to a method of producing specific pathogen free (SPF) pigs, and more particularly to a method of artificial insemination for 2 weeks after piglets are obtained from sows.

Regarding the production method of the SPF pig, it was first disclosed in the domestic patent No. 10-0628700 filed and registered under the name of the applicant before changing the corporation name.

This patent discloses an overall technique for producing pigs with a body weight of 50 to 70 kg in an SPF facility. Here, early lactation including colostrum was milk obtained from a sterile surrogate mother, and it was difficult to mass-produce SPF pigs because they were born for 25 to 30 days of birth.

Accordingly, in order to solve the above-mentioned problems, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a method of feeding a mixed milk containing a Milk replacer, Colostrum stick and / The purpose of this study is to provide an artificial feeding method of SPF pigs for 2 weeks after birth by breeding environment.

In order to achieve the above object, the method of artificial feeding of SPF pig according to the present invention is characterized in that pigs are obtained by hysterectomy from sows and then artificial mammal is fed for 2 weeks after harvesting piglets obtained from sows according to body weight To an automatic artificial incubator provided in the SPF facility; And controlling the humidity and temperature of the room of the automatic artificial incubator and mixing the milk with a milk replacer at different mixing ratios and feeding periods to feed the isolated piglets in the first week and the second week, .

In the first week, the mammalian stage is divided into a mammalian stage on the first day, a mammalian stage on the second day, and a mammalian stage on the third to seventh day, As well as other features of the artificial feeding method of SPF pigs.

In the first week and the second week, the first day is divided into 6 hours and 12 hours is divided into 6 hours. The second week is from 12:00 to 18:00, the third day is from 18:00 to the third hour, The feeding step is divided into a fourth step and a feeding step on the first day and the second day by a predetermined time interval according to the body weight in the fourth step and the feeding step on the third day and the seventh day is performed on the first day and the second day Wherein the feeding of the food is carried out in accordance with the body weight in the same cycle as the feeding of the third to seventh days in the second week, In the fourth step, sleeping time is another feature of the artificial feeding method of SPF pig according to the present invention.

On the first day and the second day of the feeding step, a mixed flow of a mixture of 50-60 g of the dampening oil, 1 g of colostrum stick and 1 g of the vitamin is added to 500 ml of the water, 500 ml of a mixture of 61-75 g of the dredged oil, 1 g of the colostrum stick and 1 g of the vitamin preparation, and the 5th to 7th days of the feeding step were carried out by mixing three kinds Mixed feed of the SPF pigs, and in the second week, the mixed feed of four kinds of the SPF pigs according to the present invention is characterized in that 500 ml of the water is mixed with 91 to 100 g of the substitute oil and 1 g of the vitamin.

On the first day and the second day of lactation, 3.75 ml / hour of 7.5 ml / hour of piglets of 200-400 g (average 300g) or 6.75 ml / hour of piglets of 400-600 g (500 g / m 2), and the second and fourth day feeding stages were 18 ml, 400 to 600 g (average 500 g) for 200 to 400 g (average 300 g) The piglets were fed 30 ml for every three and a half hours, and on the 5th to 7th days of the feeding, 18 ml of the above three kinds of piglets were infused with 200 to 400g (average 300g) (Average 500g) in the second and third weeks, 18 ml and 400-600 g (average 500g) in the piglets of 200-400g (average 300g) Lt; / RTI > of the SPF pig according to the present invention, And the other features.

The first feeding step includes feeding the sows at a predetermined time interval according to the weight of the sows after 1 ml of glucose in the second step, Is another characteristic feature of the artificial feeding method of SPF pig according to the present invention that the feeding of 1 ml of the ferrous iron solution in the second step and the quantitative feeding at a predetermined time interval according to the body weight are performed.

1 g of the colostrum stick contained 0.66 g of carbohydrate, 0.25 g of protein, 1.6 mg of sodium, 1.2 g of vitamin D ₃ , 0.4 mg of vitamin E, 5.5 mg of vitamin C, 0.06 mg of vitamin B ₆ , 10 g of folic acid, 0.8 mg of pantothenic acid, , Calcium 5mg, zinc 0.65mg, IGF 1.68g, TGF-beta 0.12g, IgG 23.9mg, lactoferrin 1.65mg, sphingomyelin 59.8g, phosphatidylcholine 1044g, phosphatidylethanolamine 880g, phosphatidylserine 38.4g, phosphatidylinositol 591 [mu] g of DHA and 50 [mu] g of DHA are included as other characteristics of the artificial milking method of SPF pig according to the present invention.

The above-mentioned substitute oil contains not less than 20.5 g of crude protein, 15.0 g of crude fat, 3.0 g of crude fiber, 11.0 g of crude protein, 0.6 g of calcium, 1.5 g of phosphorus and 1.32 mg of vitamin A, Vitamin A 1,815 mg, vitamin D ₃ 27.5 mg, vitamin E 2,200 mg, vitamin K ₃ 400 mg, vitamin B ₂ 500 mg, vitamin B ₆ 400 mg, vitamin B ₁₂ 4 mg, folic acid 500 mg, calcium pantothenate 1,000 mg, nicotinamide 500 mg, DL methionine Of the SPF porcine artificial insemination method according to the present invention, which contains 1,000 mg, 3,600 mg of manganese sulfate, 2,000 mg of iron sulfate, 250 mg of copper sulfate, 160 mg of zinc sulfate, 80 mg of potassium iodide, 40 mg of cobalt sulfate, 20 mg of selenium and 10,000 mg of citric acid .

The indoor temperature of the automatic artificial incubator was maintained at 38 ° C and the humidity was maintained at 50 to 60%. In addition, the automatic artificial incubator periodically started at 40 ° C with a self-contained infrared heaters, And then kept at 25 ± 1 ° C for one week thereafter. This is another feature of the artificial milking method of SPF pig according to the present invention.

The temperature of the infrared heater was 43 ± 2 ° C on the first day, 42 ± 2 ° C on the second day, 41 ± 2 ° C on the third day, 38 ± 2 ° C on the fourth day, 35 ± 2 ° C on the fifth day, 33 ± 2 ° C, 31 ± 2 ° C on the 7th day, and 27 ± 2 ° C on the week after that, which is another feature of the SPF pig artificial feeding method according to the present invention.

The present invention provides a method of feeding a mixed milk in which water is appropriately blended with water in place of milk of a sterile surrogate mother for two weeks after birth according to a predetermined schedule, Can be mass-produced effectively.

FIG. 1 is a structural view showing a starting position of an artificial feeding method of an SPF pig according to an embodiment of the present invention.
FIGS. 2 to 5 are schedule charts of an artificial feeding method of an SPF pig according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings.

The method for artificial insemination of SPF pigs according to the present invention relates to a method (100) for artificial insemination for 2 weeks after obtaining piglets from sows as in Fig.

As shown in FIG. 1, the artificial feeding method of SPF pig according to the embodiment of the present invention is basically separated from the survival count of piglets obtained by hysterectomy from sows according to the body weight at birth, And controlling the indoor humidity and the temperature of the automatic artificial incubator as shown in FIGS. 2 to 5 and controlling the mixing ratio and the feeding period of the mixed oil in which water is mixed with a milk replacer , And dividing the isolated piglets into first week and second week.

Here, the SPF facility may be a breeding structure having an SPF facility and an SPF zone, respectively, according to the applicants' Korean Patent Nos. 10-0628700 and 10-0742387.

The automatic artificial incubator may comprise a plurality of rooms each equipped with an infrared heater. For example, a room having a size of 335 (W, horizontal) x 490 (D, vertical) x 350 (H, height) mm is composed of four or eight rooms, And each room may further include a HEPA filter in which 99.97% of dust is 0.3 mu m or more in size.

The indoor humidity of each room of the automatic artificial incubator was maintained at 50 to 60%, and the temperature was controlled by a self-contained infrared heater, starting at 40 ° C immediately after isolation (birth) and decreasing by 1 to 3 ° C every day Is maintained at 25 占 폚 for one week thereafter. This is because the difference in physiological response between mini-pigs and other non-rodent animals depends on the incubation temperature. Under the above conditions, starting from the temperature of the sows for one week after the isolation, the temperature is lowered at a certain temperature every day and adapted to the breeding environment, so that the sudden temperature drop can reduce the mortality of the piglets due to respiratory infections.

For this, the temperature of the infrared heater was 43 ± 2 ° C on the first day, 42 ± 2 ° C on the second day, 41 ± 2 ° C on the third day, 38 ± 2 ° C on the fourth day, 35 ± 2 ° C, 33 ± 2 ° C on the 6th day, 31 ± 2 ° C on the 7th day, and 27 ± 2 ° C for the following week.

Specifically, the step of mammalian ingestion in the first week is divided into a mammalian stage (e.g., FIG. 2) on day 1, a mammalian stage on day 2 (e.g., FIG. 3), and a mammalian stage on day 3 to 7 The steps for embedding in the second week may be carried out by the same constant humidity at the same room humidity and temperature as in Fig.

2 to 5, the first week and the second week are divided into 6-hour intervals from 6:00 to 12:00 in the first phase, 12:00 to 18:00 in the second phase, 18:00 to 0:00, The time can be divided into the third stage, the 0th stage (24:00 midnight), the 6th stage, and the fourth stage.

As shown in FIGS. 2 and 3, the first and second days of the feeding step are performed in the first to third steps as well as the fourth step (0 to 6 hours) (not shown).

2 and 3, the first mixed oil may be prepared by blending 500 ml of water with 50 to 60 g of a frying oil, 1 g of a colostrum stick, and 1 g of a vitamin preparation. For piglets between 200 and 400 g (average 300 g), it is preferable to feed 3.75 ml per hour or 7.5 ml per hour, 6.75 ml per hour or 12.5 ml per hour for piglets between 400 and 600 g (average 500 g). Here, the weight of the piglets refers to the weight at birth (hereinafter the same). When over feeding is performed, the pigs are killed due to diarrhea and digestive disorders. If the pigs are down fed, they suffer from hypoglycemia and dehydration.

Particularly, as shown in FIG. 2, the first day's milking step records data according to the delivery of the sows in the first step (from 6 o'clock to 12 o'clock), and in the second step (12 o'clock to 18 o'clock) After feeding 1 ml of glucose, it is preferable to carry out a fixed amount of feeding at a predetermined time interval according to the body weight. At this time, the glucose is orally glucose (5% of glucose), and it can be supplemented with body fluids and electrolytes of the piglets by preferentially feeding it, and also helps detoxification of toxins, thereby increasing the survival rate.

As shown in FIG. 3, the second feeding step is preferably performed at a predetermined time interval according to the body weight after injecting 1 ml of the ferrous iron solution in the second step (from 12 o'clock to 18 o'clock). This is because the stored iron at the birth of piglets is about 15 mg, and iron that can be reused from red blood cells that are destroyed every day is less than 1 mg, and about 1 mg of milk can be supplied. However, since SPF piglets are in a state of breast milk deficiency, This is because it is necessary to prevent this.

The colostrum stick was prepared to replace the colostrum obtained from conventional surrogate mothers. The colostrum stick (1 stick) contained 0.66 g of carbohydrate, 0.25 g of protein, 1.6 mg of sodium, 1.2 쨉 g of vitamin D ₃ , 0.4 mg of vitamin E, 5.5mg, 0.06mg vitamin B _6, folate 10㎍, pantothenic acid 0.8mg, 4㎍ biotin, calcium 5mg, zinc 0.65mg, IGF 1.68㎍, TGF-β 0.12㎍, IgG 23.9mg, lactoferrin 1.65mg, sphingomyelin , 1044 占 퐂 of phosphatidylcholine, 880 占 퐂 of phosphatidylethanolamine, 38.4 占 퐂 of phosphatidylserine, 591 占 퐂 of phosphatidylinositol and 50 占 퐂 of DHA.

By using the colostrum stick, it is possible to overcome the difficulty in matching the sowing time of sows and surrogate mothers in order to obtain colostrum obtained from a conventional surrogate mother. Colostrum is produced only within 24 hours after delivery. If colostrum IgG (immunoglobulin) is not supplied to piglets within 3 hours after delivery, it will be able to overcome the conventional problem of being exposed to pathogenic bacteria in the surrounding environment.

The above-mentioned substitute oil contains not less than 20.5 g of crude protein, 15.0 g of crude fat, 3.0 g of crude fiber, 11.0 g of crude protein, 0.6 g of calcium, 1.5 g of phosphorus and 1.32 mg of vitamin A, Vitamin A 1,815 mg, vitamin D ₃ 27.5 mg, vitamin E 2,200 mg, vitamin K ₃ 400 mg, vitamin B ₂ 500 mg, vitamin B ₆ 400 mg, vitamin B ₁₂ 4 mg, folic acid 500 mg, calcium pantothenate 1,000 mg, nicotinamide 500 mg, DL methionine 1,000 mg, 3,600 mg of manganese sulfate, 2,000 mg of iron sulfate, 250 mg of copper sulfate, 160 mg of zinc sulfate, 80 mg of potassium iodide, 40 mg of cobalt sulfate, 20 mg of selenium and 10,000 mg of citric acid.

As shown in FIG. 4, it is preferable that the feeding step on the 3rd to 7th days is carried out by increasing the time interval between the first and second day feeding steps and quantitatively feeding according to the 2 or 3-way mixed flow path body in which the substitute oil content is increased stepwise.

The mixed oil of the two or three kinds may be mixed with 500 to 75 g of the mixed oil, 1 g of the colostrum stick and 1 g of the vitamin, and 2 The mixed oil may be a mixture of 76 to 90 g of refined oil and 1 g of a vitamin preparation in 500 ml of water.

As a specific example of feeding amount, on the 3rd and 4th day of the feeding step, 18 ml of milk of 200 ml to 400 g (average of 300 g) of the two kinds of mixed piglets is sampled every three and a half hours, piglets of 400 to 600 g (Average 500g) in the piglets of 200-400g (average 300g) every three and a half hours, 18ml, 400-600g (average 500g) in the three- Of piglets can be fed 30 ml every three and a half hours.

As shown in FIG. 4, in the third to seventh days, the feeding step has a sleeping time to reduce feeding in the fourth step (0 to 6 hours), and in the second week, the third to seventh days It is preferable to feed the four-kind mixed flow quantity feed according to the body weight in the same cycle as the feeding step, and to have only the sleeping time in the fourth step.

Here, the above-mentioned four kinds of mixed oil is higher in the oil content than the above-mentioned three kinds of mixed oil, and it may be a mixture of 91 to 100 g of oil and 500 g of water and 1 g of vitamin.

The specific feeding amount of the second week is 18 ml, and 400 to 600 g (for example, 300 ml) for the piglets of 200-400 g (average 300g) for three and a half hours, Average 500g) piglets can be fed 30ml every three and a half hours.

Each of the above-described mixing steps may be performed by self-feeding and / or hand feeding. Here, the self-feeding is a case where the health condition of the piglets is good and the mixed milk can be ingested alone, and the hand feeding is a case in which the child is less self-fed because the body weight is less than 300 g or the health condition is poor.

The average survival rate at 14 days was 68.5%, which was higher than the conventional 60.7% when artificial feeding was carried out for 2 weeks by the artificial feeding method of SPF pig described above. The surrogate mother can overcome difficulties such as adjusting the sow and delivery time, And the basis for mass production.

100: Artificial mammal blocks for 2 weeks after piglets were obtained from sows

Claims (10)

In a method for artificial mammalian embryos for 2 weeks after obtaining piglets from sows by hysterectomy,
Isolating the piglets obtained from the sows according to body weight and isolating them with an automatic artificial incubator provided in the SPF facility; And
Controlling the indoor humidity and temperature of the automatic artificial incubator, and mixing the milk with a milk replacer by mixing the water with the mixing ratio and the feeding period, and storing the isolated piglet in the first week and the second week Respectively,
The indoor humidity of the automatic artificial incubator was maintained at 50 to 60%, and the temperature was controlled by the self-contained infrared heaters at 40 ° C, 1 ° C to 3 ° C daily, and 28 ° C on the 7th day of isolation. Lt; RTI ID = 0.0 > 25 1 C < / RTI >
The method according to claim 1,
In the first week, the mammalian stage is divided into a mammalian stage on day 1, a mammalian stage on day 2, and a mammalian stage on day 3 to 7,
Wherein the second feeding step is carried out in the same constant humidity at the same room humidity and temperature.
3. The method of claim 2,
In the first week and the second week, the first day is divided into 6 hours and 12 hours is divided into 6 hours. The second week is from 12:00 to 18:00, the third day is from 18:00 to the third hour, Is divided into a fourth step,
The first and second mammalian feeding steps are carried out at a predetermined time interval according to body weight in the fourth step,
The third to seventh day of mammalian feeding is performed by increasing the time interval between the first and second day of feeding, depending on the body weight. In the fourth step, the feeding and sleeping times are reduced,
Wherein the second week is a fixed amount of feeding according to body weight at the same cycle as the third to seventh day of the feeding step, and only the sleeping time is provided in the fourth step.
The method of claim 3,
On the first day and the second day, 500 ml of the above-mentioned water was mixed with one kind of a mixture of 50 to 60 g of a dredged oil, 1 g of a colostrum stick and 1 g of a vitamin preparation,
On the 3rd and 4th days of the feeding step, 500 ml of the above water was fed with a mixture of 61-75 g of refined oil, 1 g of colostrum stick and 1 g of a vitamin preparation,
On the 5th to 7th days of the feeding step, 500ml of the above water was mixed with a mixture of three kinds of the mixture of 76-90g of the substitute oil and 1g of the vitamin preparation,
In the second week, a mixed flow of four kinds of blended oils is mixed with 500 ml of water and 91 to 100 g of a mixed oil and 1 g of a vitamin preparation.
5. The method of claim 4,
On the first day and the second day of lactation, 3.75 ml / hour of 7.5 ml / hour of piglets of 200-400 g (average 300g) or 6.75 ml / hour of piglets of 400-600 g Or 12.5 ml feeds every two hours,
On the 3rd and 4th days, the milking step was carried out by feeding 18 ml of milk to the piglets of 200-400 g (average 300 g) every three and a half hours, 30 ml feeding of piglets of 400-600 g and,
On the 5th day to 7th day, the milking step was carried out for 18 ml for every three and a half hours in the piglets of 200 to 400 g (average 300 g), 30 ml Feeding,
In the second week, 18 ml of milk is added to piglets of 200-400 g (average 300 g), and 30 ml of piglets of 400 to 600 g (average 500 g) is fed every three and a half hours. How to artificially feed SPF pigs.
The method of claim 3,
The first feeding step is a step of feeding the sows in the first step and feeding the sows in a predetermined amount of time according to the weight of the sows,
Wherein the feeding step is carried out by feeding 1 ml of a ferrous iron solution in the second step at a predetermined time interval according to the body weight.
5. The method of claim 4,
1 g of the colostrum stick contained 0.66 g of carbohydrate, 0.25 g of protein, 1.6 mg of sodium, 1.2 g of vitamin D ₃ , 0.4 mg of vitamin E, 5.5 mg of vitamin C, 0.06 mg of vitamin B ₆ , 10 g of folic acid, 0.8 mg of pantothenic acid, , Calcium 5mg, zinc 0.65mg, IGF 1.68g, TGF-beta 0.12g, IgG 23.9mg, lactoferrin 1.65mg, sphingomyelin 59.8g, phosphatidylcholine 1044g, phosphatidylethanolamine 880g, phosphatidylserine 38.4g, phosphatidylinositol Lt; RTI ID = 0.0 > of DHA < / RTI >
5. The method of claim 4,
The crude oil contains 20.5 g or more of crude protein, 15.0 g or more of crude fat, 3.0 g or less of crude fiber, 11.0 g or less of query, 0.6 g or more of calcium, 1.5 g or less of phosphorus and 1.32 mg or more of vitamin A,
The above-mentioned vitamin medicines include vitamin A 1,815 mg, vitamin D ₃ 27.5 mg, vitamin E 2,200 mg, vitamin K ₃ 400 mg, vitamin B ₂ 500 mg, vitamin B ₆ 400 mg, vitamin B ₁₂ 4 mg, folate 500 mg, calcium pantothenate 1,000 mg, Characterized in that it contains 500 mg of amide, 1,000 mg of DL methionine, 3,600 mg of manganese sulfate, 2,000 mg of iron sulfate, 250 mg of copper sulfate, 160 mg of zinc sulfate, 80 mg of potassium iodide, 40 mg of cobalt sulfate, 20 mg of selenium and 10,000 mg of citric acid. Feeding method.
delete 9. The method according to any one of claims 1 to 8,
The temperature of the infrared heater was 43 ± 2 ° C on the first day, 42 ± 2 ° C on the second day, 41 ± 2 ° C on the third day, 38 ± 2 ° C on the fourth day, 35 ± 2 ° C on the fifth day, 33 ± 2 ° C, 31 ± 2 ° C on the 7th day, and 27 ± 2 ° C on the following day.

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