KR100721644B1 - Functional food containing silkworm extract for the promotion of alcohol metabelism - Google Patents

Abstract

According to the present invention, after extracting the pulverized silkworm chrysalis from 12 to 13 days in a blender for 2 to 3 hours with ethanol for 2 to 3 hours, concentrated by filtration (60 mesh) to obtain silkworm chrysalis ethanol extract, and the residue of the second And extracted with purified water at 80 ~ 100 ℃ filtered (60 mesh), concentrated and then frozen and dried manufacturing method of silkworm pupa hot water extract, characterized in that

A health functional food having a hangover action by simultaneously enhancing the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), which are the silkworm pupa hot water extract as an active ingredient. will be.

Silkworm pupa hot water extract, hangover relief food

Description

Functional food containing silkworm extract for the promotion of alcohol metabelism

1 is a graph of alcohol dehydrogenase (ADH) activity,

2 is a graph of acetaldehyde dehydrogenase (ALDH) activity.

The present invention relates to a functional food for hangover relief according to alcohol metabolism promoting action containing silkworm extract, silkworm pupa hot water extract.

In Korea today, people habitually drink alcohol to socialize or to relieve the stress of office workers. 30% of Korean adults are addictive drinkers, and 10% of them are known as alcoholics. Based on the total alcohol consumption, it is reported that one bottle of liquor per person per year, that is, two bottles of soju per person per week, is based on 100% net alcohol.

This high consumption of alcohol is 7 kcal per gram of energy source, it is an energy source that helps sterilization and blood circulation, while showing addiction, habit, and dependence on alcohol, resulting in central nervous system, circulatory system, digestion Toxic effects such as mechanical, vascular, and endocrine systems cause various diseases.

Due to health problems caused by drinking alcohol, there is a great interest in the hangover before and after drinking. Traditional foods used to relieve hangover in Korea include sunjik soup, bean sprouts soup, northern fish soup, Korean country, oyster, vegetable juice, persimmon leaf tea, green tea, coarse salt, gun chestnut, etc. Cucumbers, spinach, lotus root, pine needles, ginseng, north fish, plum and aloe vera are known. In addition, Park Jong-cheol et al. (2) studied the effects of parsley extract on alcohol metabolism process and active ingredients, and Jeong-cheol et al. (3) presented the effects of aloe extract on alcohol metabolism.

Alcohol metabolism is shown in Table 1, categorized by metabolism, and the contents of each metabolism are as follows.

1) Ethanol oxidation by alcohol dehydrogenase (ADH) and oxidized nicotinamide adenine dinucleotide (NAD ⁺ ).

Mainly alcohol metabolism is the process of NAD ⁺ is reduced to NADH by alcohol dehydrogenase (ADH) to become acetaldehyde (acetaldehyde). In the next step, it is metabolized to acetone by acetaldehyde dehydrogenase (ALLDH), where acetaldehyde, which accumulates due to metabolic disorders, is toxic and mutagenic and carcinogenic. And is involved in repair and causes tumors.

2) Microsomal ethanol-oxidizing system (MEOS).

This reaction, which occurs in the liver microsomes, is the second most important alcohol metabolic pathway, which is mainly metabolized by chronic alcohol intake. In this metabolism, cytochrome (CYP2E1) acts nutritionally to produce kiache ketone bodies, while detoxifying alcohol produces free radicals and produces toxic and carcinogens that harm human health.

3) Reduced nicotinamide adenine dinucleotide phosphate (NADPH) Co-reaction with oxidase and catalase.

This is not a major metabolic pathway but a hydrogen peroxide-dependent reaction for alcohol metabolism in MEOS, where NADPH oxidase and catalase jointly metabolize hydrogen peroxide. Hydrogen peroxide is produced by NADPH oxidase, so NADPH is directly metabolized for alcohol oxidation, but indirect MEOS alcohol metabolism by metabolizing hydrogen peroxide produced by catalase.

4) Co-reaction with Xanthine oxidase and catalase.

Similar to the co-reaction by NADPH oxidase and catalase, hydrogen peroxide-dependent reaction for alcohol metabolism in MEOS, indirectly as xanthine oxidase and catalase jointly metabolize hydrogen peroxide. It is a reaction to metabolize alcohol.

Table 1. Alcohol Metabolic Pathways

Furthermore, looking at the effects of alcohol on the antioxidant system,

Animals and alcoholics exposed to alcohol develop immune reactions with alcohol metabolites such as acetaldehyde and hydroxyethyl free radicals in the liver. Radical attack on the immune system produces lipid peroxides, resulting in protein denaturation. Alcoholic liver disease is caused by oxidative damage caused by malondealdehyde (MDA), a lipid peroxide produced by alcohol consumption. You get

Then, looking at antioxidants and disease prevention,

Oxidative stress is a chronic disease that manifests itself as it ages, and oxidative stress causes other chronic diseases such as heart disease and cancer.

1) Definition of Free Radical

Free radicals can be defined as "a substance in which one or more unpaired electrons are present." In this case, it is very unstable in terms of energy and very strong in responsiveness, and by obtaining electrons from surrounding molecules, forming electron pairs, oneself gets stability. But the material that reacts with the radicals again has an unpaired electron and becomes itself a free radical. One free radical thus causes a chain of redox reactions to occur.

In the process of obtaining ATP (see Table 2), oxygen loses its electrons in the mitochondria and has a very unstable free group, which is highly unstable because the electrons do not form a dipole, resulting in high activity due to the nature of obtaining electrons in other orbits. Have

Table 2. Generation of hydroxy radicals from oxygen (Generation of Hydroxyl Radical from O ₂ )

O _2- ^. = Superoxide radical, OH. = Hydroxyl radical.

2. Factors for Creating Free Radicals

Oxygen is the most abundant element on earth (53.8%), accounting for 21% of the dry atmosphere, and aerobic organisms acquire energy through breathing with this rich oxygen as an electron acceptor. However, the ground state triplet oxygen, an oxygen but stable molecular state that is absolutely necessary for life support, has various physical, chemical and environmental factors such as enzyme system, reduction metabolism, chemicals, pollutants, and photochemical reactions. such as by (see Table 3) superoxide radicals ^{_{(superoxide radical, O 2 -)}} , hydroxyl radicals (hydroxyl radical, and HO), hydrogen peroxide _{(hydrogen peroxide, H 2 O 2} ), singlet oxygen (singlet oxygen, ¹ When converted to highly reactive active oxygen such as O ₂ ), it has a double sided effect that causes fatal oxygen toxicity in the living body.

In other words, these free radicals are non-selective and irreversible destructive action on cell components such as lipids, proteins, sugars, DNA, etc. as well as aging, cancer, stroke, Parkinson's disease, brain diseases, ischemia, arteriosclerosis It is known to cause various diseases such as skin diseases, digestive diseases, inflammation, rheumatism, autoimmune diseases. In addition, lipid peroxides resulting from lipid peroxidation by these free radicals also cause various functional disorders due to oxidative destruction of cells, thereby causing aging and disease.

Table 3. Factors for Creating Free Radicals

3) Enzymes on Antioxidant System

In humans, substances that cause oxidative stress can occur. Superoxide anion (O 2- ^. ) Is released into the cytoplasm when oxygen is incompletely reduced in the electron transport system in the mitochondria. Superoxide radicals are relatively less reactive in themselves, but the presence of metals such as iron or copper makes them very reactive and histotoxic hydroxyl radicals (OH) ^. . Xanthine oxidase can also generate superoxide anions upon reperfusion of ischemic tissues. Inflammatory cells such as phagocytic cells and neutrophils also produce hydrogen peroxide and hypochlorous acid to kill bacteria. These oxidants can damage surrounding cells and cause tissue damage following inflammation. Drugs such as acetaminophen are also hepatotoxic because they increase the production of free radicals in the metabolism of the drug. Smoking produces a lot of free radicals, which can lead to oxidative damage that can seriously harm your health. However, the production of these free radicals occurs under normal circumstances, so without an antioxidant defense mechanism, we cannot survive. Therefore, our bodies have various forms of antioxidant defenses against normal oxidative stress. As shown in Table 4, SOD (superoxide dismutase) and GPX (glutathione peroxide) are enzymes that act as antioxidants by inhibiting radical formation, and SOD (superoxide dismutase) is copper (Cu), zinc (Zn) and manganese ( Subtype of Mn). Free radicals are produced when oxygen is incompletely reduced in the electron transport system in the mitochondria. As shown in Table 5, copper and Zn-SOD are superoxide radicals produced by oxidizing oxygen by acting on cytosol. It converts superoxide radicals into hydrogen peroxide. The produced hydrogen peroxide is decomposed into water and oxygen by catalase and simultaneously converted into water by GPX (glutathione peroxide). Meanwhile, Mn-SOD plays a role in mitochondria, and its role is to convert superoxide radicals produced from oxygen into hydrogen peroxide, which is converted into water and oxygen by catalase. At this time, GPX (glutathione peroxide) will act simultaneously. Other antioxidants include vitamin C, vitamin E, flavonoids, and cartenoids, which act as radical scavengers, as shown in Table 4. Antioxidants that have a role in healing damage include lipases, proteases, DNA repair enzymes, and transferases.

Table 4 Antioxidant Enzymes and Substances

SOD = Superoxide Dismutase, GPX = Glutathione Peroxidase,

Dashed line = suppression.

Table 5. In vivo Reactive Oxygen Species (ROS) Removal Reactions

ER = endoplasmic reticulum, ETC = electron transport chains, OX = oxidases, endogenous antioxidants are bolded: GSH = reduced glutathione, GSSG = oxidized glutathione, GR = glutathione reductase, GPX = glutathione peroxidase, SOD = superoxide dismutase.

On the other hand, silkworm chrysalis, a natural nourishing gangjeong food in Dongbogam, is called a sleep dragon in Chinese medicine. And there is also a drug that can eliminate diabetes or roundworms. A brief look at the life history of silkworm pupa water-soluble extract is as follows. Silkworms are fully transformed insects whose first generation overwinters as eggs after four periods of egg, larva, pupa and moth. The silkworm eggs hatch after a certain period of time to become ant silkworms, and ant silkworms fall asleep after eating mulberry leaves for about 3-4 days. As such, the first state of sleep is called 1 side, and the silkworm at this time is called 1 side sleep. 1 side sleeps for about 16 hours, take off the fault and become silkworm 2nd. A silkworm is a four-time sleep that usually sleeps four times. Silkworms become 5 years old and eat mulberry leaves for about 7-8 days. The silkworm at this time is called ripe silkworm. Ripe silkworms are made in cocoons for 2-3 days, and then into pupa, and then chrysalis in 10-15 days to moths to break through the cocoon to spawn mating and finish the first generation. Silkworm pupa has been shown to reduce the atherosclerosis index by 30% as a result of feeding unsaturated fatty acids extracted from silkworm pupa into experimental rats that induced hyperlipidemia. In addition, it has been reported that the skin's moisturization is improved by more than 50% compared to the non-oiled result after applying the unsaturated fatty acid after removing the oil from the skin to cause dry skin. And it has been experimentally proved that male silkworm chrysalis extracts and isolates a protein that promotes the synthesis of cGMP, which is known as one of the male's erectile promoting ingredients, to rescue its structure and enhance its erectile promoting activity.

On the other hand, the main components of the mulberry leaf feeding silkworms are moisture, protein, carbohydrates, fats, inorganic salts, vitamins, etc., and the physiological activity of the mulberry leaves lowers the hypoglycemic effect and lowers the amount of neutral lipids that cause obesity. It has been reported to lower the amount of, and inhibits the generation of active oxygen which is the cause of aging by about 20% and at the same time increases the activity of free radical scavenging enzyme by about 14% is reported to be effective in inhibiting aging. In addition, silkworms contain abundant essential amino acids, and according to the report of the Food Development Institute, alanine among essential amino acids promotes alcohol metabolism to help hangover and improve liver function.

An object of the present invention is to develop and provide a health functional food for hangover elimination according to alcohol metabolism promoting action containing silkworm pupa hot water extract, 8 weeks old to administer alcohol to reveal the alcohol detoxification effect on silkworm pupa hot water extract By comparing the activity of alcohol degrading enzymes in the liver of ICR mouse, it was confirmed that the hangover elimination effect of the present invention is superior to existing products, and completed the present invention.

The present invention relates to a hangover rehabilitation health functional food using a silkworm pupa hot water extract as an active ingredient, and more particularly, a hangover relief health function consisting of an effective amount of silkworm pupa hot water extract and at least one carrier It is food.

Silkworm pupa hot water extract of the present invention, after extracting the powder pulverized silkworm pupa of the 12 to 13 days in a blender for 2 to 3 hours without any discrimination between males and females for 2 to 3 hours, filtered and concentrated by 60 mesh sieve, silkworm pupa ethanol extract Then, the residue was secondly extracted with purified water to 80 ~ 100 ℃, filtered through a 60 mesh sieve, concentrated and then lyophilized silkworm pupa hot water extract.

Acceptable carriers in terms of formulation may be used food additives that can be added conventionally in food.

For example, liquid formulations may be conventionally formulated with silkworm pupa hot-water extracts, water-soluble calcium, including red beet, DHA, taurine, oligosaccharides, liquid fructose, citric acid, β-cyclodextrin, vitamins, and purified water. .

In tablet form, silkworm pupa hot water extract, excipients such as calcium carbonate and lactose, binders such as corn and starch, lubricants such as disintegrants, stearin and talc can be conventionally formulated.

In the form of a capsule, it may be hard capsuled together with silkworm pupa hot water extract and excipients such as calcium carbonate and kaolin, or emulsified using an oil solvent such as oil to form a soft capsule.

In addition, it can be formulated into granules, syrups and the like.

The dosage of the functional food according to the present invention may vary depending on the age, weight, general health, sex, meal, time of administration, rate of excretion, combination of drugs, the degree of disease during treatment, etc. An adult silkworm lyophilizate based on 60 kg body weight can be taken up to 100-500 mg per day.

Hereinafter, preparation examples and experimental examples according to the present invention are described.

Formulation example

1) Preparation of silkworm pupa hot water extract

Silkworm pupa hot water extract is 1 Kg of silkworm pupa on 12th to 13th day without any distinction between male and female, pulverized in a blender, first extracted with 80% ethanol (EtOH) for 2 hours, filtered through 60 mesh sieve, and the filtrate is evaporated. (Evaporator) to obtain a silkworm pupa ethanol extract, and then the residue was extracted twice with 500 ml at 80 ℃ with purified water, filtered through 60 mesh sieve and the filtrate was lyophilized for 7 days to obtain silkworm pupa hot water extract. See Table 6)

Table 6. Sample yield

N0. Sample name (Sample name) Scientific name (Science name) Yield (w / v,%) One Silkworm pupa hot water extract Bombyx mori (silkworm) 0.9 2 Silkworm pupa ethanol extract Bombyx mori 0.8

2) soft capsule

Prescription (mg) in 1 soft capsule:

Silkworm pupa hot water extract 100

Palm oil 40

Beeswax 15

Lecithin 15

Soybean Oil 220

Total 390mg

Method of manufacture:

After preparing a suspension by homogeneously mixing the composition amount of the formulation, it is prepared by filling in a gelatin soft capsule.

Experimental Example 1. Experiment for Measuring Alcohol Degrading Enzyme Activity

1. Experimental Materials

Silkworm pupa hot water extract of the product used in this experiment was used silkworm pupa hot water extract prepared according to Formulation Example 1, alcohol is a product of 00 Co., Ltd. Was used.

2. Experimental Animal

The animals used in this study were 8-week-old male mice of ICR system and weighed 19-22 g. The feed was experimented after adapting to the laboratory environment for 2 weeks while supplying enough 0000 (stock) of solid feed and water. The temperature of the animal cage was 24 ± 2 ℃, the humidity was 40-50%, and the contrast was 12 hours.

3. Experimental method

1) Acute Alcohol Induction Experiment

In order to observe the effects of acute alcohol induction, 8 animals were tested (Normal) (negative control: distilled water), Control (positive control: distilled water + alcohol (J company, Chamois)), 4 kinds of the present invention Treatment group KW1-KW4 (Silkworm pupa hot water extract + alcohol (J company, Chamois)), Drink group (B company, Dawning, 10-fold dilution + Alcohol (J company, Chamois)), Alcodex group (Alcodex) (A company, 10-fold dilution of alcodex + alcohol (J company, Chamois)) (see Table 7). The animals were fasted 24 hours before the test, and each sample was orally administered. To induce alcohol metabolism, alcohol (J Company, Chamois, 2 g / kg) was administered with a test sample (silk pupa hot water extract) (5%) 30 Minutes later orally (see Table 7). Two hours after alcohol administration, anesthesia was used to remove the liver. The liver was isolated by lysis with physiological saline. The isolated liver was prepared as enzyme solution for antioxidative and alcoholase activity experiments.

2) Determination of Liver Alcohol Degrading Enzyme Activity in vivo

① Determination of alcohol dehydrogenase (ADH) activity

The extracted liver was homogenized at 4 ° C. with 0.25 M sucrose buffer (pH 7.4) at 7 times the weight. Homogenate was centrifuged at 700 rpm for 10 minutes and 4,000 rpm for 10 minutes, and the supernatant was further centrifuged at 50,000 rpm for 1 hour to make the supernatant a cytosolic ADH (alcohol dehydrogenase) enzyme source.

Determination of ADH enzyme activity was performed by Lebsack et al. According to the method described above, the rate of NADH production was performed as an index at 340 nm (see Biochem. Pharmac. (26) pp 1151-1154 (1976) and Kor. J. Pharmacogn. 29 (2) pp 120-124 (1998)). ). The ADH activity was initiated by adding a substrate at 37 ° C. and expressed as inactive U / mg protein with 1 unit of coenzyme formation per unit of 1 uM per minute. The composition of the reaction solution was ethanol as a substrate, 2.6 ml of 0.1 M Tri-HCl buffer (pH 8.5), 0.2 M ethanol 0.1 ml, 0.05 M semicarbazide HCl 0.1 ml, 0.1 A mixture of 0.02 ml of M NAD (in 0.01 M hydrochloric acid) and 0.1 ml of enzyme source was added thereto, and then adjusted to a total of 3 ml by adding an inhibitor (rotenone) as an inhibitor. The limit value of the test group in which only the substrate was removed was defined as the enzyme activity.

② Measurement of acetaldehyde dehydrogenase (ALDH) activity

Pellets at 4,000 rpm were washed twice with 15 ml of sucrose buffer, suspended with 1.15% KCl of twice the volume of liver weight, and then again 0.3% sodium decay per 1 g of liver weight. Oxycholate (sodium deoxycholerate) was added and centrifuged at 50,000 rpm for 1 hour, and the supernatant was measured as a mitochondrial ALDH (acetaldehyde dehydrogenase) enzyme source.

The activity test for ALDH was performed as an index of NADH production rate at 340 nm as well as ADH activity measurement. The ALDH activity was initiated by adding a substrate at 25 ° C. and expressed as inactive U / mg protein with 1 unit of coenzyme formation per unit of 1 uM per minute. The composition of ALDH reaction solution was 1.25 ml of 0.2 M-tris HCl buffer (pH8.3), 1 M KCl 0.1 ml, 0.1 M pyrazole, 0.02 ml, using propionaldehyde as a substrate. 0.02 ml of 1 M 2-mercaptoethanol, 0.06 ml of 0.1 M propionaldehyde, 0.1 ml of 0.1 M NAD (in 0.01 M hydrochloric acid) and 0.1 ml of enzyme source are added and an inhibitor is added. Add and adjust to a total of 2.5 ml and then carry out the reaction. The limit value of the test group in which only the substrate was removed was defined as the enzyme activity. Quantification of enzyme protein was carried out by Lowry et al.

4. Data Analysis

Statistical analysis of all experimental results was performed using SAS statistics program (SAS institute, 1998). In order to verify the antioxidant activity and alcohol detoxification effect of each sample was analyzed by ANOVA, the significance between groups was significant at p <0.05 using Duncan's multiple range test. Verified.

5. Results and Discussion

1) Determination of Liver Alcohol Degrading Enzyme Activity in vivo

① Determination of alcohol dehydrogenase (ADH) activity

ADH activity can be seen as the difference between the optical density (OD) value when no ethanol was added and the optical density (OD) value when ethanol was added. In the case of%, the negative group (NC) was 1.21 times, silkworm pupa hot water extract KW4 (0.5 mg / ml) was 1.74 times, the drink (dawn) was 1.59 times, Alcodex was 1.19 times, and KW4 was ADH. The highest activity was shown, and KW4 (0.5 mg / ml) showed the highest ADH activity. In addition, ADH activity was 1.4 times higher at 0.5 mg / ml than alcohol only from 500 minutes to 50 minutes after the addition of ethanol. Table 7, FIG. 1

Table 7. Effect of silkworm pupa hot-water extract, drink and alcodex on hepatic ADH activity in acute alcohol induced mice.

 Sample  Administered Route  ADH activity; absorbance (O.D), (%)  0-50 (minutes)  50-500 (minutes)  Control Group Normal group (NC) Oral (p.o.) 0.178 ± 0.061 (121.09) 0.05 ± 0.07 (100) Positive group (PC) Oral (p.o.) 0.147 ± 0.049 (100) 0.05 ± 0.045 (100)   Sample Group Invention (KW1) Oral (p.o.) 0.143 ± 0.125 (97.28) 0.06 ± 0.139 (120) Invention (KW2) Oral (p.o.) 0.153 ± 0.066 (104.08) 0.07 ± 0.08 (140) Invention (KW3) Oral (p.o.) 0.136 ± 0.05 (92.52) 0.05 ± 0.044 (100) Invention (KW4) Oral (p.o.) 0.255 ± 0.07 (174.47) 0.07 ± 0.075 (140) Drink Oral (p.o.) 0.234 ± 0.085 (159.18) 0.06 ± 0.08 (120) Alcodex Oral (p.o.) 0.176 ± 0.095 (119.73) 0.06 ± 0.103 (120)

All values are expressed as mean ± standard deviation.

Samples were administered 30 minutes before ethanol administration.

Mean values with different alphabets in the same furnace show significance at p <0.05.

Negative control (NC): distilled water (D.W)

Positive group (PC; positive control): distilled water (D.W) + alcohol (alcohol) (J company, Chamois dew)

Alcodex Group: (A Company, Alcodex; 10 Bae Hee-Seok) Alcodex + Alcohol (J Company, Chamois)

Drink Group: (B Company, Dawning; 10 Bae Hee-Seok) Drink + Alcohol (Alcohol) (J Company, Charm Dew)

Invention KW1-KW4 group: Silkworm pupa hot water extract 4 groups (KW1: 0.01 mg / ml, KW2: 0.05 mg / ml, KW3: 0.1 mg / ml, KW4: 0.5 mg / ml) + alcohol (alcohol) (J company, Chamois)

* The other groups except NC were orally administered with alcohol (alcohol) (J company, Chamois) 30 minutes after the oral sample of each sample.

<Calculation method>

In terms of optical density (O.D) results: The difference in absorbance values from 0 to 50 minutes and the difference in absorbance values from 50 to 500 minutes are obtained. The difference can be used to compare the magnitude of enzymatic activity.

② Measurement of acetaldehyde dehydrogenase (ALDH) activity

ALDH activity was primarily determined by the difference between the optical density (OD) value when no substrate (propionaldehyde) was added and the optical density (OD) value when the substrate (propionaldehyde) was added. When the optical density (OD) is 100, normal group (NC) is 7.8 times, drink group (Drink) (dawn) is 5.6 times, Alcodex (Alcodex) is 4.8 times, silkworm The pupa hot water extract (0.5 mg / ml) group (KW4) showed 11.2 times higher activity than the KW 4 (Drink) and Alcodex (Alcodex) groups. In four concentrations of silkworm pupa hot water extract group (KW), KW 4 of 0.5 mg / ml showed the highest ALDH activity. When ALDH activity was observed from 50 minutes to 500 minutes after adding ethanol, silkworm extract tended to have higher ALDH activity than alcohol alone, especially 0.05 among silkworm pupa extracts. KW2, a concentration of mg / ml, showed slightly higher ALDH activity than KW4, but overall, the activity of silkworm pupa hot water extract (0.5 mg / ml) group (KW4) was the best (Table 8, Figure 2).

Table 8. Effect of silkworm pupa hot-water extract, drink and alcodex on hepatic ALDH activity in acute alcohol induced mice.

 Sample  Administered Route  ALDH activity; Absorbance (O.D), (%)  0-50 (minutes)  50-500 (minutes) Control Group Normal group (NC) Orally (p .o.) 0.039 ± 0.013 (780) 0.041 ± 0.061 (186.36) Positive group (PC) Orally (p .o.) 0.005 ± 0.016 (100) 0.022 ± 0.054 (100)  Sample Group Invention (KW1) Orally (p .o.) 0.028 ± 0.02 (560) 0.049 ± 0.092 (222.73) Invention (KW2) Orally (p .o.) 0.049 ± 0.02 (980) 0.065 ± 0.054 (295.45) Invention (KW3) Orally (p .o.) 0.043 ± 0.017 (860) 0.056 ± 0.037 (245.55) Invention (KW4) Orally (p .o.) 0.056 ± 0.021 (1120) 0.062 ± 0.088 (281.82) Drink Orally (p .o.) 0.028 ± 0.01 (560) 0.029 ± 0.018 (131.82) Alcodex Orally (p .o.) 0.024 ± 0.015 (480) 0.037 ± 0.033 (168.18)

All values are expressed as mean ± standard deviation.

Samples were administered 30 minutes before ethanol administration.

Mean values with different alphabets in the same furnace show significance at p <0.05.

Negative control (NC): distilled water (D.W)

Positive group (PC; positive control): distilled water (D.W) + alcohol (alcohol) (J company, Chamois dew)

Alcodex Group: (A Company, Alcodex; 10 Bae Hee-Seok) Alcodex + Alcohol (J Company, Chamois)

Drink Group: (B Company, Dawning; 10 Bae Hee-Seok) Drink + Alcohol (Alcohol) (J Company, Charm Dew)

Invention KW1-KW4 group: Silkworm pupa hot water extract 4 groups (KW1: 0.01 mg / ml, KW2: 0.05 mg / ml, KW3: 0.1 mg / ml, KW4: 0.5 mg / ml) + alcohol (alcohol) (J company, Chamois)

* The other groups except NC were orally administered with alcohol (alcohol) (J company, Chamois) 30 minutes after the oral sample of each sample.

Based on the above results, when comparing the normal group (NC) not fed with alcohol and the group fed with alcohol only (PC), alcohol degrading enzyme activity was decreased in the group fed with alcohol only (PC), so that alcohol decomposed after alcohol ingestion It was found that the enzyme acted, and compared with each extract, silkworm pupa hot water extract helped the function of alcohol degrading enzyme and showed high alcohol degrading enzyme activity. In general, alcohol detox drinks and drugs on the market increase the activity of alcohol dehydrogenase (ADH), whereas acetaldehyde dehydrogenase (ALDH) activity is less affected, silkworm according to the present invention Chrysalis hot water extract (KW extract) enhances the activity of ALDH as well as ADH, and is thought to prevent hangover and prevent liver damage. High ALDH activity is not only good for hangover, but also prevents the accumulation of acetaldehyde in the liver. ADH decomposes alcohol into acetaldehyde, and ALDH quickly decomposes acetaldehyde into acetic acid and water to relieve hangovers and will not damage the liver. Therefore, silkworm pupa hot water extract containing (KW) product of the present invention can be seen to be superior to other existing products to hangover.

Claims (3)

After extracting the pulverized silkworm chrysalis from 12 to 13 days in a blender for 1 to 2 hours with ethanol for 2 to 3 hours without any discrimination between males and females, filtered through a 60 mesh sieve and concentrated to obtain silkworm chrysalis ethanol extract, and then the residue was purified secondly. After extracting with 80 ~ 100 ℃ filtration, concentrated with 60 mesh sieve, freeze, dried silkworm pupa hot water extract prepared as an active ingredient, alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase Health functional food for hangover relief that increases the activity of (acetaldehyde dehydrogenase; ALDH). The health functional food of claim 1 further comprising one or more pharmaceutically acceptable carriers. delete

Images