KR100717891B1 - Cosmetic composition of anti-itching of atopic dermatis - Google Patents

Abstract

The present invention is mixed with extracts of natural herbs such as myth, thistle, turmeric, schisandra chinensis, burdock, etc. in order to alleviate the itching associated with atopic dermatitis, extracts of herbs that relieve or relieve each step such as itching, inflammation, microbial infection When applied to a patient, the present invention relates to a cosmetic composition for alleviating the symptoms of atopic dermatitis, wherein the herb extract selected from the group consisting of Xinhua extract, thistle extract, turmeric extract, Schisandra chinensis extract, burdock extract, or a mixture of two or more thereof is 0.1 To 20% by weight and the balance comprises a carrier.

Shin Ewha, Thistle, Turmeric, Burdock, Atopy

Description

Cosmetic composition exhibiting an itching effect associated with atopic dermatitis {Cosmetic composition of anti-itching of atopic dermatis}

The present invention relates to a cosmetic composition exhibiting an itching relief effect associated with atopic dermatitis. More specifically, the present invention mixes extracts of natural herbs such as myth, thistle, turmeric, schisandra chinensis, burdock, etc. in order to alleviate the itching associated with atopic dermatitis to relieve or relieve each step such as itching, inflammation, microbial infection, etc. When the extracts of herbs are applied to a patient, the present invention relates to a cosmetic composition for alleviating the symptoms of atopic dermatitis.

Atopic dermatitis is a recurrent chronic dermatitis with severe pruritus, the cause of which has not been clearly identified to date, but 10-20% of the world's population suffers from it. Turned out. In addition, the incidence of atopic dermatitis continues to rise in recent decades due to an increase in allergens of air-mediated allergy due to environmental pollution and an increase in conditions for forming dry skin. In Korea, the number of patients is increasing.

Although atopic dermatitis is thought to respond by an allergic reaction involving immunoglobulin E (IgE), the cause of sure onset is still not clearly identified. That is, atopic dermatitis often involves an increase in blood levels of IgE and an increase in eosinophils. From an immunological point of view, as the amount of IgE in the serum increases as inflammation increases in atopic dermatitis patients, there is a continuing discussion about whether this antibody is associated with atopic dermatitis. However, only a response to IgE is accepted in a broad sense as a reaction to the formation of urticaria (urticaria) on the skin. However, it is thought that dung margin does not change into an eczema reaction even if it is strongly scratched, and in case of skin with a deficit disorder, an eczema reaction is easily formed by scratching. Recent studies, however, have clearly confirmed that direct eczema can be caused by the action of IgE. In tests of patients with IgE antibodies against tick antigens, more than half of them showed positive reactions. When IgE antibodies were injected and allergens were applied to mice, IgE antibodies and allergens reacted to produce cytoactive substances in mast cells. It appeared to emit. This reaction is pathologically eczema with infiltration of lymphocytes and eosinophils and is a delayed response by IgE antibody. It can be considered that this immunological eczema forms atopic dermatitis. Although atopy itself belongs to type I allergy, atopic dermatitis is a pathological reaction similar to eczema and contact dermatitis, suggesting the involvement of type IV allergy (delayed type). In addition, it has been suggested that delayed-type reactions involving eosinophilic or lymphocyte infiltration play an important role in the onset and chronicization of atopic dermatitis (Iwamoto et al., J. Leukoc. Biol., 52, pp572- 578 (1992)). ; Frigas et al., J. Allergy. Clin. Immunol., Pp 527-537 (1986)).

The term atopy has a dictionary meaning of clinical hypersensitivity or allergy, which is inherently strange or inappropriate.

Persons with atopic dermatitis show urticaria response to a large number of antigens when skin tests on protein antigens, such as house dust, mites, animal hair, pollen, and food, are common in the environment.

Atopic dermatitis occurs in about 10 to 20% of the entire Korean population, especially in children. After 5 years of age, the incidence rate decreases, and many adults disappear or relieve the symptoms. Demonstrate the characteristics of As industrialization progresses rapidly, the probability of exposing external stimulants due to a clean living environment is lowered. Thus, the number of patients with atopic dermatitis due to the immune system is increasing by more than 10% every year. Although no definite model of the cause of the disease is known, the amount of immunoglobulin E (IgE) increases rapidly and the amount of gamma interferon decreases in atopic dermatitis patients.

In order to improve the skin of atopic dermatitis, a lipid complex of ceramide essential fatty acid cholesterol, which repairs the skin barrier, is applied by a method such as application (Korean Patent Laid-Open Publication No. 2001-0044622), oral use of an antihistamine to prevent itching, The application of steroids for anti-inflammatory and the like is generally used, taking and applying the essential fatty acid gamma-linolenic acid (Korean Patent Publication No. 2000-0046633), the application of oligosaccharides to improve the skin immune status (Korean Patent Publication No. 2000-0029754), the application of ginkgo biloba extract (Korean Patent Publication No. 1999-0076619), a composition for regulating skin immunity containing Shin-Ihwa extract (Korean Patent Publication No. 2003-0025975), but is satisfactory There was a lot of room for improvement because of no effect.

An object of the present invention is to mix the extracts of natural herbs such as myth, thistle, turmeric, Schisandra chinensis, burdock, etc. in order to alleviate the itching associated with atopic dermatitis, to relieve or alleviate the steps of itching, inflammation, microbial infection, etc. When the extract is applied to a patient, to provide a cosmetic composition to alleviate the symptoms of atopic dermatitis.

Cosmetic composition showing an itching relief effect associated with atopic dermatitis according to the present invention, the herb extract, thistle extract, turmeric extract, Schisandra chinensis extract, herb extract consisting of a mixture of burdock extract and skin, lotion, cream And a commercially known cosmetic composition selected from the group consisting of essences, shampoos, toothpastes, detergents, soaps.

Hereinafter, the present invention will be described in detail with reference to specific examples.

Cosmetic composition showing an itching relief effect associated with atopic dermatitis according to the present invention, the herb extract, thistle extract, turmeric extract, Schisandra chinensis extract, herb extract consisting of a mixture of burdock extract and skin, lotion, cream , Essence, shampoo, toothpaste, detergent, soap, characterized in that comprises a commercially known cosmetic composition selected from the group consisting of. That is, the present invention is characterized by providing a cosmetic composition exhibiting the alleviation effect of atopic dermatitis by mixing extracts of herbs known to have excellent anti-inflammatory and anti-inflammatory effects among natural herbs.

In the above, it means "myth", that is, the bud before flowering. Magnolias are magnolia, peony flower, white magnolia, magnolia, purple magnolia, Japanese magnolia, and magnolia, and the common magnolia is white magnolia from China. Magnolias are long, 6 to 9 petals long, and can be easily distinguished by the purple light streaks below the outside of the flowers. Shinyi refers to a bud that has not yet bloomed, and has been used as a medicine for a runny nose, especially sinusitis for a long time. The extract of Magnolia Biondii Bark Extract is obtained by adding alcohol to Magnolia Biondii Bark, that is, magnolia bud, and heating to obtain an extract, and then purifying the extract through column chromatography, and then freezing and drying the purified extract. To obtain in the form of a powder.

Thistle is common in the fields and fields, usually grows from 30cm to 1m, and in spring, the leaves are eaten with herbs. Thistle contains taracarterin acetate. Stigmasquerol. Alpha. It contains beta amylin, clears blood and stops hemostasis. Anti-inflammatory action. So, high blood pressure is very effective if you complement the juice of thistle vinegar, and if you have a bruise and drink it immediately, it can be painful. Another habitual bleeding nosebleed. It is known that drinking thistle juice is also very good for uterine bleeding and various respiratory inflammations and enterocolitis. However, pregnant mothers should not drink, and it is known that the vigor is very good when the elderly are drunk. The thistle extract (Silybum Mariaum Extract) is added to alcohol thistle (Silybum Mariaum), heated to obtain an extract, and then purified through column chromatography, the purified extract is obtained in the form of a powder by lyophilization. .

The turmeric, according to the pharmacist and torture, said, 'The turmeric is bitter. To shed blood; Used for bleeding, bleeding, and blood clots. Merchants often deceive turmeric as turmeric ', which is a herb that has been widely used in medicine since ancient times. Turmeric is a perennial herb that grows in the tropics, and its roots are solid or branched round pillars. It grows in mountainous areas. Its roots are stored in the winter, and they are planted in the spring in the field. The components of turmeric include 1 to 3% of yellow cattle, 1 to 5% of essential oils, 30 to 40% of starch, and some oil. Yellow cow is curcumin the main ingredient. The main components of the essential oils are turmeric and dehydroturmeron, about 50%, gingeryrene, dialphafellandrene, cineol, alpha sabinel and borneo. Turmeron is an unstable ingredient that gives the herb an unusual smell and is easily converted into alpha and gamma turmeron. Root stem powder or decoction has been found in animal experiments to promote diarrhea, gastric secretion, diuretic, detoxification, galactose treatment, reduce urobilinin, and promote the secretion and excretion of hot water. And it promotes the regeneration of hepatocytes. This action is believed to be due to the action of curcumin-like substances and essential oils. Curcumin also has a strong bactericidal action. p-Torylmethylcarbinol is known as a good hepatitis drug known as a natural compound with an antiallergic effect. Essential oils are also known to have strong bactericidal activity and to dissolve gallstones. Root stem extract has been shown to have a good therapeutic effect on chronic hepatitis, cholecystitis, biliary tract disease, and cholelithiasis in clinical trials. In oriental medicine, it is used as a fragrance or placebo medicine, and also used for nosebleeds, hemostasis, and hematuria. It separates curcumin and essential oils and is used for fragrance, stomach diuretic, antipyretic, digestive, acute cholecystitis, cholangitis, catarrhal jaundice, cholelithiasis and acute hepatitis. Curcuma Longa (Turmeric) Root Extract is added to the roots of Curcuma Longa or other homologous plants, heated to obtain an extract, and then purified through column chromatography. The extract is lyophilized to obtain the form of a powder.

In the above, Schisandra chinensis is a deciduous vine plant that grows in mountain valleys of various places, especially in all areas. Leaves are alternate, wide oval, with small toothed teeth on edges. Flowers are biphasic, bloom in June, slightly reddish yellowish white. Fissure fragments 6 to 9 Medicinal is strong sour fruit. It is called Gaomija without hair on the back of leaf. Schizandrin, Gomisin A-Q, Citric, αYlangene, Matic acid, Citric acic, etc. It is used for self, vivid dyeing, and inserting branch. It is also used for processing such as Schisandra tea, Schisandra juice, Schisandra wine. Pharmacological actions of Schizandra chinensis include central nervous excitability, antitussive action, expectoration, uterine contraction, antibacterial action, blood pressure lowering action, cardiovascular action, and transaminase lowering action. It is known. The Schisandra chinensis extract (Camellia Sinensis Root Extract) is obtained by adding alcohol to the root of Schisandra chinensis, heating to obtain an extract, and then purifying it through column chromatography, and then lyophilizing the purified extract to obtain a powder form. .

Burdock is a two-year-old herb that grows in mountain fields all over Korea and blooms from July to August. It is an edible and medicinal plant that has been cultivated in farmhouses since ancient times, and it grows wild. Country of origin is Europe. The roots are edible, the seeds are used as swelling as diuretics, and also used as an antidote for sore throats and poisons. Burdock roots contain inulin and some palmitic acid. The Burdock Root Extract (Arctium Lappa Root Extract) is obtained by adding alcohol to the root of burdock or other homogenous plants, heating to obtain an extract, and then purifying it through column chromatography. Obtained in form.

When the herb extract is included in less than 0.1% by weight, there may be a problem that the mitigating effect of the itch according to the present invention is not sufficient, on the contrary, more than 20% by weight, especially when the formulation is broken down into cosmetic formulations There may be a problem that the product becomes unstable.

The herbal extract in the composition may be included in an amount of preferably 5 to 15% by weight.

As the carrier, an aqueous solvent such as water or alcohol, in which the herbal extract can be stably present, may be used. Alternatively, the carrier may be any of those in which the herbal extract may be stably present without modifying the herbal extract or reacting with the herbal extract, and particularly, various cosmetic compositions or various pharmaceutically acceptable solvents may be used. Can be used. It will be apparent to those skilled in the art that other solid powdery carriers such as talc may be used.

In particular, the herbal extract as described above may be composed of a cosmetic composition including the known cosmetic composition components, wherein the effective amount of the herbal extract may be 0.1 to 20% by weight.

The commercially known cosmetic composition ingredients may be components that make up skin, lotion, cream, essence, shampoo, toothpaste, detergent, soap, and the like.

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Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Examples 1-6

Xinhua extract was cut into 10 kg of magnolia buds with a crusher, and 100 kg of 70% ethanol was added thereto, and extracted by heating at 80 ° C. for 2 hours using a device equipped with a reflux device. Thereafter, the extract was filtered using a filter aid (trade name: AW celite NF Media, manufacturer: Advanced Minerals Corporation, manufacturer nationality: USA). After ethanol was removed by concentration under reduced pressure, purified water was adjusted to 50 kg. 50 kg of the mixture was passed through a column equipped with an XAD-7 adsorption resin (trade name: XAD-7, manufacturer: Eichrom technologies Inc., nationality: USA) to selectively organic matter near molecular weight of 1,000 daltons. This part was adsorbed by using the property of adsorbing. After separating the active ingredients adsorbed on the resin with 70% ethanol, ethanol was removed by concentration under reduced pressure, and lyophilized to obtain the mythic extract in powder form (500 g).

Thistle, turmeric, Schisandra chinensis and burdock obtained the same extracts in the same way, with the turmeric using the roots of turmeric or other homogenous plants, Schisandra chinensis roots, and burdock roots of burdock or other homologous plants. Was used. The yield of thistle extract was 400 g, the yield of turmeric extract was 500 g, the yield of Schisandra chinensis extract was 500 g, and the yield of burdock extract was 300 g.

Using the extracts as described in Table 1 below to obtain a lotion-type cosmetic composition that can be applied to the skin.

For comparison, as a comparative example, except that it does not contain any extract according to the present invention as described above, the remaining ingredients were prepared together with the same cosmetic composition, and used in the experiment.

Raw material name Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative example Stearic acid 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Stearyl alcohol 2.5 2.5 2.5 2.5 2.5 2.5 2.5 1,3-butylene glycol 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Monostearic acid glycol 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Potassium hydroxide 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Glyceline 4.0 4.0 4.0 4.0 4.0 4.0 4.0 cholesterol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Carboxy Vinyl Polymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Xinhua Extract 2.0 0.0 2.0 2.0 2.0 2.0 0.0 Thistle extract 2.0 2.0 0.0 2.0 2.0 2.0 0.0 Turmeric extract 2.0 2.0 2.0 0.0 2.0 2.0 0.0 Schizandra Extract 2.0 2.0 2.0 2.0 0.0 2.0 0.0 Burdock Extract 2.0 2.0 2.0 2.0 2.0 0.0 0.0 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount

The oil and water components of the above components were heated to 75 ° C., respectively, and then emulsified using a homogenizer as a cosmetic mixer, followed by degassing, filtration and cooling.

Experimental Example 1

Efficacy effect experiment on itching relief

In order to confirm the atopic dermatitis alleviation effect on the cosmetic composition prepared in Examples 1 to 6 and Comparative Examples, 70 refractory atopic patients from 2 to 20 years old were classified into 7 groups and 10 patients were applied to each group. After 1 minute, 10 minutes, and 1 hour after the subject was asked whether there was an itching, the answer was obtained, and the number of people who felt itching is shown in Table 2 below. The definition of itching was defined as the condition in which the hand went more than twice at the site of the itch.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative example Itching after 1 minute 2 4 5 5 5 4 10 Itching after 10 minutes 3 6 6 7 6 6 10 Itching after 1 hour 3 7 7 7 6 6 10

In the above experiment, the effect on the itch of Example 1 showed an itch relief effect of 80% after 1 minute, Examples 2 and 6 are 60%, and Examples 3 to 5 are 50% itch relief effect It was shown that the comparative example had no itch relieving effect.

The results after 10 minutes and after 1 hour were also shown to be excellent in the synergy effect for the various extracts in Example 1 was excellent, it was determined that the composition according to the present invention showed an excellent effect on the relief of itching.

Experimental Example 2

Measures the inhibitory effect of TNF-α production

Whether to induce TNF-α production for Examples 1 to 6 and Comparative Examples was investigated as follows. Mast cell line HMC-1 was cultured in culture medium IMDM / FBS at 5% CO ₂ , 37 ° C., and then aliquoted to 3 × 10 ⁵ cell lines. In order to stabilize the cells, the cells of the compositions of Examples 1 to 6 and Comparative Example were pre-incubated for 10 minutes before treatment at 37 ° C., and the aqueous solutions of Examples 1 to 6 and Comparative Examples were respectively 0.01, 0.1 and 1.0 mg / ml (distilled water). ELISA (enzyme linked immunosorbent assay) was performed to investigate the protein level of TNF-α in the supernatant so as to dissolve 0.01, 0.1, and 1.0 mg of the compositions of Examples 1 to 6 and Comparative Examples per ml.

Anti-human TNF (1 μg / ml), a monoclonal antibody against TNF-α, was diluted with PBS (pH 7.4), coated in 100 μl each in a 96-well plate, and left for 12 hours at room temperature. The plates were washed with PBS containing 0.05% of Twin and then blocked for 1 hour with PBS containing 1% BSA, 5% sucrose and 0.05% NaN ₂ . After washing several times, the supernatant obtained by centrifugation and recombinant TNF-α to be used as a standard curve were added, and then left at 37 ° C. for 2 hours. Each well was then washed again, and TNF-α (0.2 μg / ml) bound to secondary antibody biotin was added and left at 37 ° C. for 2 hours. The wells were washed and then avidin-linked peroxidase was added and left at 37 ° C. for 20 minutes. The wells were washed again, then ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)) substrate was added, and the color reaction was measured at 405 nm using an ELISA reader, and the results were measured. It is shown in Table 3 below.

TNF-α Secretion TNF-α inhibition rate (%) Example 1 0.196 ± 0.016 64.22 Example 2 0.216 ± 0.013 32.72 Example 3 0.213 ± 0.013 33.50 Example 4 0.214 ± 0.015 31.87 Example 5 0.214 ± 0.013 32.53 Example 6 0.209 ± 0.012 33.54 Comparative example 0.256 ± 0.014 -

As shown in Table 3, it was confirmed that the composition of Example 1 according to the present invention most inhibits TNF-α production at 1 mg / ml.

Therefore, according to the present invention by mixing the extracts of natural herbs such as myth, thistle, turmeric, Schisandra chinensis, burdock, etc. in order to alleviate the itching associated with atopic dermatitis Herbs that relieve or relieve each step such as itching, inflammation, microbial infection When the extracts of the present invention is applied to a patient, there is an effect of providing a composition for alleviating the symptoms of atopic dermatitis.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

Claims (7)

delete delete delete Commercialization selected from the group consisting of skin, lotions, creams, essences, shampoos, toothpastes, detergents, soaps as 0.1-20% by weight and the remaining amount of herbal extracts consisting of a mixture of xinhua extract, thistle extract, turmeric extract, schizandra extract and burdock extract Cosmetic composition showing the itching relief effect associated with atopic dermatitis, characterized in that comprises a known cosmetic composition composition. delete delete delete