KR100695537B1 - Cosmetic material composition including Rhododendron schlippenbachii root - Google Patents

Abstract

The present invention relates to a cosmetic composition having an effect of improving the healing of atopic dermatitis or eczema containing azalea root extract.

Azalea root, Atopic dermatitis, Eczema, Anti-allergic

Description

Cosmetic composition containing azalea root extract {Cosmetic material composition including Rhododendron schlippenbachii root}

1 and 2 are photographs showing the atopic dermatitis improving effect of the present invention

Figure 3 is a photograph showing the eczema improving effect of the present invention.

The present invention relates to a cosmetic composition having a skin disease-improving effect containing a natural extract, and more specifically, Rhododendron schlippenbachii Maxim.var. It relates to a cosmetic composition having an effect of improving healing.

Azaleas are distributed in Manchuria and Ussuri, and are deciduous shrubs on the outcrop of sandy or mountainous ridges in Korea.

The leaves are revived, gathered at the end of 5 branches, rosette-shaped, broad ovate, flat at the edges, greenish, young hairy, but later disappeared, and the backside is light green, hairy on the veins.

Flowers bloom simultaneously with leaves, light pink, fragrant and toxic. The upper part of the corolla has brown spots, with 10 honeys, 1 pistil, and the fruit is sulcus, hairy, ovate. The flowering period is from April to May and the fruiting season is from October.

As a pharmacological action, it is known to use flowers for hypertension and swelling, but the action on atopic dermatitis or eczema of the present invention is unknown.

The term atopy tends to easily form immunoglobulin E, an immune substance that causes an allergic reaction, and refers to a case where the genetic tendency to cause disease-specific asthma, high fever, allergic rhinitis, and atopic dermatitis. Atopic dermatitis is a chronic dermatitis that develops in people with such atopic dermatitis. It begins with infant eczema, which is called fever, and has a chronic and recurrent course. Subjective symptoms are characterized by severe pruritus, depending on the time of onset, the characteristic distribution and appearance of the lesions, sometimes accompanied by allergic rhinitis, asthma and other history of atopic diseases or family history. Various skin irritation can be a deteriorating factor in atopic dermatitis, and relatively well known ones include excessive bathing, excessive soap use, dry skin due to low humidity conditions, mother's underwear, coarse chemical fiber products, towel bath, etc. Skin irritation, psychological tension, skin infections, sweat and fever, and foods (milk, eggs, wheat, nuts, seafood, etc.). In addition, various antigens such as house dust mites, animal hair and secretions may act as aggravating atopic dermatitis. These foods, pollen, dust and other inhalable agents are sometimes proved by skin tests or serum tests, but the results are not absolute because of false positive and false negative reactions.

Atopic dermatitis is characterized by the characteristics of skin lesions and their distribution according to the age of the patient. Infant (up to 2 years), childhood (3-11 years), adolescence (12-20 years) and adult (after 20 years) Divided into three periods. In infancy, they appear as red, moist, crusty lesions on the head, face, and trunk. In childhood, dry skin lesions such as papules, thymus glands, and scales appear in areas where the skin is folded, such as arms, legs, wrists, and ankles. do. In adolescence and adulthood, there are lesions such as papules, scales, pigmentation, and lichenitis in the head, face, neck, torso, limbs and limbs. These symptoms usually improve with age, but sometimes severe dermatitis can persist until adulthood. The main symptom of atopic dermatitis is itching. The patient feels itching more easily than a normal person and is sensitive to minor irritation. When itching to scratch, the skin develops into eczematous lesions, and as the lesion progresses, a more severe pruritus is caused again, which causes more severe scratching, causing a series of vicious cycles.

Eczema dermatitis is one of the most common skin diseases and refers to an inflammatory reaction of the skin caused by various factors of exogenous and endogenous, and in many cases it is difficult to identify the cause. The disease is diagnosed by visual appearance and can be classified according to the causative factor of the distribution or cause of the lesion. Eczema is usually accompanied by varying degrees of pruritus and can be divided into acute and chronic. In the acute phase, bullous papules, erythema, and edema appear, and in severe cases, exudative lesions occur. Eczema dermatitis is somewhat different in its classification, but it can be classified into three types, namely, external factors, internal factors, and other unknown causes. Eczema dermatitis caused by external factors includes primary irritant dermatitis and allergic contact dermatitis, phototoxic and photoallergic contact dermatitis, polymorphic photo-induced dermatitis, infectious eczema dermatitis, and internal eczema dermatitis caused by drugs. Eczema dermatitis, self-sensitizing or self-eczema lesions, and ulcerative dermatitis, and other causes of unknown eczema dermatitis include atopic dermatitis, seborrheic dermatitis, molecular eczema, neurodermatitis, herpes zoster, white nasal eczema, dry eczema, infant eczema , Malnourished dermatitis and deprived keratin exfoliation. When atopic dermatitis or eczema develops, patients are treated with steroid ointments and oral medications (including injections) in hospitals, clinics and pharmacies.However, when steroids are used as oral or injectables, subcutaneous blood, pigmentation, hair loss, itching, Side effects such as facial erythema have been reported, such as side effects of endocrine system, secondary adrenal insufficiency, diabetes, side effects of digestive system, peptic ulcer, gastritis, mental nervous system side effects, depressive state, headache, musculoskeletal side effects, osteoporosis, protein It is known that metabolic reactions cause squeaking dogs, nitrogen imbalance, electrolyte side effects, blood pressure rise, hypertension in eyes, glaucoma, etc.If steroid-based ointment is used, skin infections, steroid acne, steroid dermatitis, and other parts of the pituitary gland Has serious side effects such as suppression of neocortical function. Reported.

The inventors of the present invention to solve the above problems, in order to develop cosmetics that are effective in atopic dermatitis and eczema using natural herbal medicines, a histamine glass inhibitory activity of the azalea root extract during the study through antihistamine and antibacterial activity And found to possess both antimicrobial activity, containing azalea root extract as an active ingredient confirmed that the effect of treating atopic dermatitis or eczema without causing side effects when applied to the skin and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition that is effective in treating atopic dermatitis or eczema.

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Hereinafter, the present invention will be described in more detail.
Azalea root extract for treating atopic dermatitis or eczema of the present invention possesses anti-inflammatory and anti-itch action that inhibits histamine activity and antibacterial action for preventing bacterial infection.

5 to 20 times of distilled water, methanol or ethanol or a mixed solvent thereof is added to the flowers, leaves and / or roots of azaleas, and then extracted twice at 50 to 100 ° C. for 5 to 10 hours, then filtered and concentrated under reduced pressure. Add.

The cosmetic composition according to the present invention is characterized in that it contains 0.01 to 50% by weight, preferably 0.1 to 10% by weight of the azalea root extract prepared above.

The cosmetic composition for treating or improving atopic dermatitis or eczema of the present invention may have a conventional formulation, for example, formulated with softening longevity, astringent longevity, nourishing longevity, nutrition cream, body lotion, body oil, body essence, etc. Can be. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. In the following, representative skin and lotion compositions of the composition for treating atopic dermatitis or eczema will be described, for example, representative base ingredients and additives.

In addition, the cosmetic composition for treating atopic dermatitis or eczema according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the skin condition or taste of the user.

Hereinafter, the cosmetic composition for treating atopic dermatitis of the present invention will be described in more detail with reference to examples of the present invention, but the present invention is not limited to these examples.

Example 1 Preparation of Water Extract of Azalea Root

10 L of distilled water was added to 1 kg of dried azalea roots, followed by repeated extraction for 8 hours at 95 ° C., filtered through a 400 mesh filter cloth, filtered through a filter of 0.45 μm, and concentrated under reduced pressure to obtain 172 g of an extract.

Example 2 Preparation of Azalea Root Methanol Extract

10 L of methanol was added to 1 kg of azalea roots, followed by repeated extraction for 6 hours at 70 ° C., followed by filtration through 400 mesh filters, 0.45 μm filter filtration and concentration under reduced pressure to obtain 131 g of an extract.

Example 3 Preparation of Azalea Root Ethanol Extract

10 L of ethanol was added to 1 kg of dried azalea roots, followed by repeated extraction for 7 hours at 80 ° C., filtered through a 400 mesh filter cloth, filtered through a filter of 0.45 μm, and concentrated under reduced pressure to obtain an extract 57 g.

Example 4 Preparation of 50 wt% Methanol Extract of Azalea Root

10 L of 50 wt% methanol was added to 1 kg of dried azalea roots, followed by repeated extraction for 6 hours at 70 ° C., followed by filtration through a 400 mesh filter cloth, filtered through a 0.45 μm filter, and concentrated under reduced pressure to obtain 153 g of an extract.

Example 5 Preparation of 50 wt% Ethanol Extract of Azalea Root

10 L of 50 wt% ethanol was added to 1 kg of dried azalea roots, and extracted twice at 80 ° C. for 7 hours, filtered through a 400 mesh filter cloth, filtered through a 0.45 μm filter, and concentrated under reduced pressure to obtain 89 g of an extract.

Example 6: Antimicrobial Effect Experiment

The antimicrobial effect of the mixed extracts was measured for Examples 1 to 5.

Antimicrobial experiments were measured by agar diffusion using a paper disc. The strain was used as the following microbial test strain. Escherichia coli (ATCC25922), Candida albicans (ATCC 10259), Staphylococcus aureus (ATCC 29737), Bacillus subtilis (ATCC 6633) are selected. It was. As a medium, trypticase soy agar was used for E. coli, and nutritic agar was used for Candida albican, Staphylococcus aureus, and Bacillus subtilis. Strains were inoculated with 100 ml of liquid medium and incubated at 32 ° C. for 20 hours to activate. The solution was inoculated in 100 μl (107-108 cfu / ml) in each medium, covered on a plate, and each extract was placed on a sterile disc plate surface and 20 μl of the sample was instilled. Incubation for 36 hours to determine the antimicrobial activity by the diameter (mm) of the inhibitory ring formed around the disk (Table 1).

TABLE 1

Example 7: Antihistamine Activity Test

Lung tissue (approximately 2.8 g per horse) was isolated from 10 guinea pig females (approximately 250 g) to remove adipose tissue, bronchus and blood, followed by a tea containing Ca ²⁺ , Mg ²⁺ and 0.1% gelatin. Enzyme treatment (5 mg / ml collagenase, 1.8 unit / 27 μl elastase) was performed for 15, 15 and 25 minutes using Lloyd's buffer (TGCM buffer). Each enzyme treatment was filtered through a nylon mesh (nylon mesh) and a metal mesh (metal mesh) (100㎛) and centrifuged (called simple dispersed mast cells). The pellets are suspended in 16 ml of Ca ²⁺ , Mg ²⁺ -free, 0.1% gelatin-containing buffer (TG buffer) and loaded into rough percoll (1.041 mg / ml density). The pellet was centrifuged at 1500 rpm for 20 minutes to obtain pellets. The cells were suspended in 8 ml of TG buffer and loaded into discontinuous Percoll (1.06-1.10 mg / ml density) and centrifuged at 1500 rpm for 20 minutes to separate them into various cell layers. Since mast cells exist mainly in layers 3 and 4, cells of this layer were obtained and washed twice with TGCM buffer. Whole cells and mast cells were stained with trypan blue and alcian blue, respectively, and the number of cells was measured under a microscope to measure the purity of mast cells. Got it.

After elucidating IgG1 antibody (anti-OVA 1ml / 106 cells) to mast cells (4105 cells) and reacting for 45 minutes at 37 ° C, the cells were washed with TGCM buffer to remove the anti-OVA antibody not bound to the mast cell membrane. . The cells were suspended in 1 ml TGCM and then pretreated for 5 minutes with each concentration of drug (substance to be searched). Histamine was measured in the supernatant after centrifugation after cooling with ice for 10 minutes by sensitization with 1.0 μg / ml OVA (ovalbumin).

The amount of histamine liberated in each sample is modified by the method of Siraganian, which is based on automated continuous-flow extraction and fluorometric analyzer (Astoria analyzer series 300, Astoria-pacific international). , Oragon, USA). Prepare a 1N-hydrochloric acid, 0.73M phosphoric acid, 5N sodium hydroxide, 1N sodium hydroxide, saline diluent and sampler wash, o-phthalaldehyde solution and connect the tube connected to the analyzer. After ligation, the histamine stock solution was diluted to 20 ng, 10 ng, 5 ng, 3 ng, and 1 ng to obtain concentration-dependent results of the standard curve, and then each sample was diluted with 2% perchloric acid to measure the amount of histamine. The amount of histamine contained in each sample was expressed as a percentage of the amount of histamine contained in astronomically used cells (Table 2).

TABLE 2

Formulation Example 1 and Comparative Example 1: Preparation of skin lotion having a therapeutic effect on atopic dermatitis of the present invention

A skin lotion was prepared at the composition ratio as shown in Table 3 below.

[Table 3] Prescription of skin lotion

Test Example 1 Test for Confirming the Atopic Dermatitis Improvement Effect of the Skin Lotion

Two children who had atopic dermatitis for more than three years and who received hospital treatment but did not receive any effects were examined. The skin lotion of Formulation Example 1 was applied to the skin twice a day for 14 days in the morning and evening for 14 days on the hips (Fig. 1) and legs (Fig. 2), the site of the most severe atopic dermatitis, and improved atopic condition. Confirmed. As shown in the photographs on the right after 14 days compared to before application of Formulation Example 1 in each photograph on the left in FIG. 1 and FIG. 2, severe atopic symptoms were completely improved.

Example 9 Preparation of Milk Lotion Having an Atopic Dermatitis Treatment Effect

A skin lotion was prepared at the composition ratio as shown in Table 4 below.

[Table 4] Prescription milk lotion

Test Example 2: Confirmation test to improve the atopy healing effect of the present invention milk lotion

Twenty four patients aged 7 to 18 who had atopic dermatitis for more than 2 years were examined for atopic improvement. The same person washes the milk lotion of Formulation Example 2 on the left leg and the milk cream of Comparative Example 2 on the right leg every evening, and then applies it to the skin once a day for 14 days and then improves the atopic condition to confirm the improvement of atopic condition. The degree was measured.

Table 5

Test Example 3: Confirmation test for improving the eczema healing of the milk lotion of the present invention

Eczema patients were applied to the site of the milk lotion of Formulation Example 2 twice a day for 14 days in the morning and evening for 14 days, and then confirmed the improvement of atopic condition. Milk lotion of Formulation Example 2 was applied to the skin twice a day for 14 days in the morning and evening for 14 days on the back of the hand, the site of the most severe eczema of the patient, and the improvement of eczema was confirmed. Surprisingly, eczema symptoms were completely improved, as shown in the right photographs after 14 days, compared to before application of Formulation Example 2 in the left photograph of FIG. 3.

As can be seen from the above, the azalea root extract of the present invention has antibacterial and antihistamine activity and can be used as a cosmetic since it has an effect on the healing of atopic skin.

As described above, the composition of the present invention exhibits excellent antihistamine activity without the risk of side effects because it is a natural substance, and can be used as a medicine or health functional food for the treatment and prevention of allergy, asthma, or cold.

Claims (3)

Cosmetic composition for treating atopic dermatitis or eczema comprising azalea root extract extracted with at least one polar solvent selected from water, methanol or ethanol. The cosmetic composition for treating atopic dermatitis or eczema according to claim 1, comprising 5 wt% of the azalea root extract. delete

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