KR100680140B1 - A method of manufacturing feed and water additives by using pig placenta and use of feed containing extract of pig placenta - Google Patents

Abstract

The present invention relates to a method for producing feed additives and negative additives using pig placenta, and has an effect of providing a method for preparing pig placenta extract for animal negative or feed additives that can promote animal growth. Porcine placenta extract of the present invention can be used as an animal's negative or feed additives to produce high quality meat.

Porcine Placenta Extract, Negative Additives, Feed Additives

Description

A method of manufacturing feed and water additives by using pig placenta and use of feed containing extract of pig placenta}

Figure 1 shows the process of producing the sap and feed additives using the pig placenta of the present invention.

Figure 2 is a graph showing the weight gain (Daily gain, A) and feed conversion rate (B) of ICR (Institute of Cancer Research, USA) mice grown after the negative administration of each test substance for 9 days.

Figure 3 shows the growth change in pigs fed pig placenta extract feed additives (0.2% and 0.4% (w / w) addition).

The present invention relates to a method for producing feed additives and negative additives using pig placenta. More specifically, the present invention relates to a method for producing animal feed additives and negative additives capable of producing high quality meat using pig placenta.

Placenta is called “Jaha” for herbal wood and Dongbobom and has been used as a herbal medicine and is currently applied to many products such as aging prevention, liver function improvement, and health supplements as an expensive raw material due to various pharmacologically active functions. It is becoming.

Therefore, the present inventors have developed a product that can be applied to livestock using pig placenta, which is not currently used in farms and not used in human placenta, and consequently contributed to the production of high quality pigs. Grain, which is the main feed for livestock, contains many amino acids, but relatively few sulfur-containing amino acids act as limiting amino acids for livestock growth. Reduced feed intake due to heat, disease or excessive stress in livestock, such as disease or encroachment, can result in a deficiency of the restricted amino acids, which can lead to disease deficits in the animal's immune system and consequently lead to growth stagnation. In addition, high saturated, unsaturated fatty acids and amino acids, organic chelating minerals are expensive to form in the market, it is difficult to artificially add a large amount to the feed.

Accordingly, it is an object of the present invention to provide a method for preparing animal feed additives or negative additives that can increase the animal's growth ability using pig placenta.

The object of the present invention is to prepare an extract for the sap and feed additives using pig placenta, to analyze the components of the sap, and to treat the mice with the sap and commercial products to compare the increase rate and feed requirements, the feed An extract for the additive was added to the feed of the mouse to investigate the growth of the mouse and was achieved by serum analysis.

The present invention comprises the steps of preparing the extract for the sap and feed additives using pig placenta; Analyzing the components of the fluid and growth change in the mouse; And, by adding the extract for feed additives to the feed consists of a growth change investigation and serum analysis step in the mouse.

Method of producing a pig placenta extract for the negative additive of the animal of the present invention is characterized by consisting of the following steps:

Acid hydrolysis of pig placenta at 110 ° C. for 5 hours,

Filtering the hydrolyzate to remove solids,

Placenta extract, from which solid matter was removed, was neutralized by treatment with anion exchange resin after concentration under reduced pressure.

Neutralizing pig placenta extract is prepared by filtration and sterilization for 15 minutes at 121 ℃.

Pig placenta extract prepared according to the above method has the effect of promoting the growth of animals.

Porcine placenta extract for animal feed additives of the present invention, after the acidic hydrolysis of the pig placenta at 110 ℃ for 5 hours to adjust the placental hydrolyzate to pH2 using NaOH, the placental hydrolyzate is 1: It is characterized by being prepared by mixing 1 to dry the moisture content to 10% or less.

When added to the animal feed additives placenta extract for animal feed additives 0.2 to 0.4% of the feed weight of the present invention when fed to the animal has the effect of promoting the growth of the animal.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are merely illustrative of the present invention and the present invention is not limited thereto.

EXAMPLE

Example  1: pig placenta extract manufacturer

The pig placenta was collected from a brother farm (Gimcheon, Gyeongbuk). After thawing the frozen placenta, it was washed by removing cord blood and feces. 1 kg of washed placenta was mixed with 500 ml of 3N HCl, and hydrolyzed at 110 ° C. for about 5 hours. The complete process was confirmed by the biuret and trichloroacetic acid reactions. After confirming the protein negative reaction, the solid material was removed by filtration with an 8㎛ filter. The placenta extract from which the solid material was removed was concentrated under reduced pressure and neutralized by treatment with an anion exchange resin (pH 7). The neutralized pig placenta extract was removed with residual foreign substances with 0.45 μm and 0.2 μm filters, and finally, sterilized for 15 minutes at 121 ° C. to prepare a final pig placenta extract solution, and the crude protein content was measured using Kjeldahl method. And it was used for the mouse negative administration experiment.

Placenta extract was used for feed addition using placenta collected from sibling farm (Gyeongbuk Gimcheon) and Hanaum farm (Gyeonggi Yongin), and 1 kg of washed placenta was mixed with 500 ml of 3N HCl and hydrolyzed at 110 ° C for about 5 hours. Afterwards, the placental hydrolysis solution was adjusted to pH 2 using NaOH. Placental hydrolysate adjusted to pH2 was mixed 1: 1 with Corncorp and dried as moisture content below 10% and used as feed additive. The components are as follows (Tables 1 and 2).

Analysis of free amino acid (A), mineral (B) and heavy metal (C) components of pig placental extract (PPE) (A) Porcine Placenta Extract Item Free amino acid (%) Nitrogen content (0.2%) Item Free amino acid (%) Nitrogen content (0.2%) L-Aspartic acid L-Threonine L-Serine L-Glutamic acid L-Proline L-Glycine L-Alanine L-Valine L-Isoleucine L-Leucine L-Trypsine 0.014 ± 0.014 0.016 ± 0.016 0.048 ± 0.008 0.051 ± 0.004 ND 0.215 ± 0.015 0.093 ± 0.005 0.010 ± 0.010 0.016 ± 0.001 0.049 ± 0.001 0.018 ± 0.007 L-Histiding L-Lysine L-Arginine L-Cysteine L-Methionine Phosphoserin Taurine L-Orinithine 1-Methyl-L-Histidine 3-Methyl-L-Histidine L-Phenylalanine ND 0.012 ± 0.012 ND ND 0.012 0.009 ± 0.009 0.016 ± 0.016 0.094 0.076 ± 0.002 0.018 ± 0.001 0.017 ± 0.003 (B) Porcine placenta extract (nitrogen content 0.2%) Mineral ppm Ca PKFeMgNaCuMnZn Salt (%) 168.55 ± 2.65 3.42 ± 0.03 43.01 ± 1.47 5.35 ± 0.35 18.42 ± 0.38 44.4 ± 0.96 0.22 ± 0.02 0.20 ± 0.01 2.52 ± 0.008 0.21 (C) Porcine placenta extract (0.2% nitrogen content) heavy metal ppm Cd Pb Cr As Hg ND ND ND ND ND

* ND: Not detectable

Fatty Acid Analysis of Pig Placental Extract (PPE) Porcine Placenta Extract (Nitrogen Content 0.2%) Item fatty acid% Myristic acid Pentadecamoic acid Palmitic acid Palmitoleic acid Magaric acid Magaroleic acid Stearic acid Oleic acid Linoleic acid Arachidic acid Eicosenoic acid Eicosadienoic acid cis-11,14,17-Eicosatrienoic acid Heneicosanoic acid Lignoceric acid DHA Butyric acid Caproic acid Capryic acid Caprylic acid Tridecanoic acid Myristoleic acid cis-10-Pentadecenoic acid Linoleladic acid r-Linolenic acid Arachidonic acid EPA Behenic acid Erucic anid cis-13,16-Docosadienoic acid Tricosanoic acid Nervonic acid 0.652 0.307 22.257 2.543 0.747 0.419 10.254 44.657 6.328 0.475 0.175 0.463 0.551 0.520 3.306 1.846------------------ gun 100

Example  2: Porcine placenta extract for mice ( PPE Negative dose experiment

Porcine placenta extract prepared in Example 1 was added to the negative water or feed to compare the effect of Product A with Company A.

Experimental Animals and Breeding Conditions

Three-week-old ICR (Institute of Cancer Research, USA) mice (Biolink) were used in this experiment for 7 days in a nursery controlled at a temperature of 21-24 ° C and a humidity of 40 ± 5% for 12 hours (hrs). Through the period of purification, 30 healthy males and females were used for the experiment, and 5 mice were distributed to each mouse cage and reared for 9 days.

Feed salary and water salary

The feed was fed a sufficient amount of 60g daily to each mouse cage by using a solid feed, pure water was supplied to the control group, and PPE (nitrogen content: 0.1%, 0.2 according to each condition) to the pure water for the control group %, 0.4%), HPE (Intestinal Placenta Extract, Nitrogen Content: 0.2%), Company A's product A was added to the negative amount of 4%. The dose was 0.2 ml per mouse on a daily basis.

Experiment method

The experiment was divided into a total of six, including the control (Table 3), and was fed for 9 days with sufficient feed and drinking water. Mortality was measured every day during the breeding period, weight change, feed intake, and drinking volume were measured for 9 days, and the change was divided by the number of individuals, and the effect was analyzed.

In addition, blood was collected at the end of the experiment to measure total protein, cholesterol, alkaline phosphatase, blood urea nitrogen (BUN), triglycerides, and immunoglobulin G (immunoglobulin G, IgG). Histological examination was performed.

Pig placenta extract negative administration experimental condition gender Dosing conditions Control PPE (0.1% N) PPE (0.2% N) PPE (0.4% N) HPE (0.2% N) A product F (number of objects) 5 5 5 5 5 5 M (number of objects) 5 5 5 5 5 5 Total number of objects Female: 30 Male: 30

Dosage Unit: 0.2ml PPE / mouse / day

Growth Analysis of ICR (Institute of Cancer Research, USA) Mice with Different Dosage Concentrations and Dose Concentrations Under Standard Breeding Conditions gender Item Dosing conditions Control 0.1% (N) PPE 0.2% (N) PPE 0.4% (N) PPE 0.2% (N) HPE A product Female Breeding days 9 9 9 9 9 9 Starting weight (g) 21.6 21.4 21.2 22 22 21.6 End weight (g) 23.6 23.6 24.0 25.2 24.8 24.0 Drinking water intake (ml / day) 5.20 5.88 5.36 6.32 6.40 6.66 Weight gain (g) 2.0 2.2 2.8 3.2 2.8 2.4 Daily weight gain (g / day) 0.22 0.24 0.31 0.36 0.31 0.26 Feed Intake (g / day) 4.05 3.86 3.93 4.10 3.92 3.85 Feed efficiency 18.41 16.08 12.68 11.38 12.65 14.80 Male Breeding days 9 9 9 9 9 9 Starting weight (g) 27.6 27.2 25.6 27.6 27.2 26.2 End weight (g) 31.2 31.2 29.2 32.4 31.6 29.6 Drinking water intake (ml / day) 6.20 7.28 6.84 7.76 6.69 6.36 Weight gain (g) 3.6 4.0 3.6 4.8 4.4 3.4 Daily weight gain (g / day) 0.4 0.45 0.4 0.53 0.49 0.38 Feed Intake (g / day) 5.08 4.91 4.43 5.06 4.83 4.34 Feed efficiency 12.70 10.91 11.08 9.55 9.86 11.42

* The numbers shown in Table 4 were bred by 5 males and females for each condition according to gender and expressed as averages.

As shown in Table 4 and FIG. 2, there was no death of the Institute of Cancer Research, USA (ICR) mice during the 9-day breeding period. As a result of analyzing daily weight gain and feed requirement after the experiment, both of the male and female showed the highest gain rate in the treatment group with high nitrogen content of placenta extract added to the negative water, and the feed demand ratio was also the lowest. When the nitrogen content of pig placenta was 0.1%, the increase rate was increased by 1.1 times in females, 1.4 times in 0.2%, and 1.6 times in 0.4%. Although males were lower than females, males also showed a 1.3-fold higher gain rate than the control group in 0.4% of the placenta extract. The placenta extract treatment (0.2% N) also showed an increase in body weight when compared to the control, and compared with the pig placenta extract treatment, the female showed the same gain rate as the pig placenta extract treatment with the same nitrogen content, but in the male placenta extract treatment. The weight gain was slightly higher than that of pig placenta extract. However, compared to A, a third-party amino acid product, the placenta extract showed higher weight gain and feed efficiency. In addition, the autopsy after the end of the experiment showed no abnormal symptoms in the control and all treatments.

Immunoglobulin G and IgG Concentrations According to Each Experimental Condition gender Item Dosing conditions Control PPE (0.1% N) PPE (0.2% N) PPE (0.4% N) Male IgG 7 or less 10.2 10 8.4 cholesterol 140 132 111 118 Female IgG 7 or less 7 or less 7 or less 7 or less cholesterol 187 108 101 111

As shown in Table 5, as a result of the analysis of the component content of each treatment section through the serum test, total protein and albumin, alkaline phosphatase, blood urea nitrogen (BUN), triglyceride, etc. did not show a difference, but cholesterol Both males and females showed relatively lower values in the treatment group than the control group, and immunoglobulin G (IgG) was also higher in the male than the control group.

Example  3: Porcine placenta extract for mice ( PPE Feed Addition Experiment

In Table 4, a negative amount of the pig placenta extract was administered to increase the weight gain of the mouse and boost the immune system. ) Was supplemented with 0.4% nitrogenous placenta extract.

Porcine placenta extract for feed addition prepared in Example 1 was mixed with an appropriate amount of powdered mouse feed and used after drying in solid form.

Experimental Animals and Breeding Conditions

Three-week-old female ICR (Institute of Cancer Research, USA) mice (Biolink), used in this experiment, were purchased in a breeding room controlled at a temperature of 21-24 ° C and a humidity of 40 ± 5% for 12 hours (hrs). Thirty healthy mice were used in the experiment after the daily purifying period, and five experiments were distributed to each mouse cage, and the experiment was repeated twice for each condition. Total breeding days were 10 days.

Feed salary and water salary

The feed was mixed with 0.4% nitrogen placental pig placenta extract at 0%, 0.4%, and 4% of the weight of the mouse feed, dried in solid form, and supplied to the mouse cage a sufficient amount of 60g daily. All nine bulbs were sufficiently supplied with pure water.

Experiment method

The experiment was repeated twice, divided into three groups, including the control group (see Table 6), and fed for 10 days with sufficient feed and drinking water. Mortality was measured every day during the breeding period. After 10 days of breeding, the weight change, feed intake and drinking water were measured.

In addition, blood was collected from each mouse at the end of the experiment to measure total protein, cholesterol, alkaline phosphatase, blood urea nitrogen (BUN), triglycerides, and immunoglobulin G (immunoglobulin G, IgG). The difference of significance was confirmed by analytical method (design expert6.0), and histopathological examination was performed by autopsy.

Experimental condition of pig placenta extract (PPE) feed addition gender repeat PPE addition amount Control 0.4% added 4% added Female One 5 5 5 Female 2 5 5 5

Comparison of Mouse Growth According to Feed Concentration of Pig Placenta Extract (PPE) Item Dosing conditions Control 0.4% added 4% added Breeding days 10 10 10 Starting weight (g) 22.3 22.0 22.6 End weight (g) 26.0 25.9 26.2 Weight gain (g) 3.7 3.9 3.6 Daily weight gain (g / day) 0.37 0.39 0.36 Feed Intake (g) 46.0 46.9 46.3 Daily feed intake (g / day) 4.60 4.69 4.63 Feed requirement 12.43 12.02 12.86

As shown in Table 7, 10-day mouse breeding with feed containing pig placenta extract showed the highest weight gain (3.9 g) and added 4% when pig placenta extract was added with 0.4% content. In the case of the control, rather than the control showed a decreasing effect.

As a result of confirming the feed demand using the weight gain and the feed intake, the lowest feed demand (12.02) was also found when the pig placenta extract was added to the mouse feed with 0.4% content.

Serum Analysis of the Effect of Porcine Placenta Extract (PPE) on Mice Item Dosing conditions Control 0.4% added 4% added Total protein 4.47 ^a 4.95 ^b 5.12 ^b albumin 3.02 ^a 3.35 ^b 3.43 ^b Alkaline phosphatase 84.75 89.00 88.25 Serum glutamic oxalate transaminase (SGOT) 117.75 143.00 135.50 Serum glutamic pyruvate transaminase (SGPT) 29.00 31.00 26.75 Blood Urea Nitrogen (BUN) 23.82b 23.15 ^b 18.10 ^a  Total cholesterol 114.25 99.25 107.50 Triglycerides 178.00 180.75 182.50 Immunoglobulin G (IgG) 7.67 and below 7.67 and below 7.67 and below

As shown in Table 8, the results of the serum analysis showed that the total protein and albumin tended to increase as the content of the pig placenta extract added to the feed increased, and the significance (p <0.05) was recognized in the statistical treatment. Blood urea nitrogen (BUN) also showed a statistically significant difference from the control, but decreased with the addition of placenta extract. However, the remaining items such as serum glutamic oxalate transaminase (SGOT), serum glutamic pyruvate transaminase (SGTG), triglyceride, alkaline phosphatase, total cholesterol, and immunoglobulin G (IgG) showed a tendency to increase or decrease between control and experimental groups. There was no significance (p> 0.05) for.

Example  4: pig placenta extract for pigs ( PPE ) PPE  Feed additive experiment

The pig feed to which the placenta extract used in this experiment was added was dried by mixing the pig placenta extract prepared according to Example 1 using the placenta collected from the brother farm (Gyeongbuk Gimcheon) and Hanaum farm (Gyeonggi Yongin) with the excipients. Post feed was used.

This experiment was conducted for 19 days after weaning using weaning pigs at the Han-Ae Hanaum Farm, 40 doses to feed pigs with placenta extract and 40 controls to feeds without pig placenta extract. Three replicates (240 heads in total) were performed in two. The pig placental feed additives added to the feed were fed at the weaning age of 0.2% and 0.4% (w / w). Before and after 19 days, the average starting weight and average ending weight of each condition were measured, and the weight gain per day was measured and the results were compared.

As shown in Figure 3A, weaning start weaning 80 pigs (three repetition) classified under two conditions and 19 days pig pig placenta extract feed additives were added to the weaning pig feed 0.2% (w / w) test results However, although the mean starting weight was 0.91 kg less than the control, the mean ending weight was 0.08 kg higher than the control.

In addition, as shown in FIG. 3B, the control group fed the pig placenta extract feed additive showed a daily weight gain of 0.3 g / day, whereas the control group showed a daily weight gain of 0.25 g / day, which was about 17% higher than the weight gain. Could be obtained from nine.

As shown in Fig. 3C and 3D, the result of feeding 0.4% (w / w) pig placenta extract feed additive to the weaning pig feed also showed 1.12kg higher weight gain in the administration, resulting in about 19% higher weight gain per day. Could get

Therefore, it could be concluded that feeding pig placenta extract feed additives to weaning pigs increased the growth rate of weaning pigs.

As described through the above embodiment, the present invention relates to a method for preparing feed additives and negative additives using pig placenta, and to a method for preparing pig placenta extract for animal negative or feed additives that can promote animal growth. It is effective to provide. Porcine placenta extract of the present invention is a very useful invention in the animal feed industry because it can be used to produce high quality meat by using as an animal negative additive or feed additive.

Claims (5)

Acid hydrolysis of pig placenta at 110 ° C. for 5 hours, Filtering the hydrolyzate to remove solids, Placenta extract, from which solid matter was removed, was neutralized by treatment with anion exchange resin after concentration under reduced pressure. Neutralizing pig placenta extract is filtered, sterilized for 15 minutes at 121 ℃ produced method of animal placenta for pig placenta extract, characterized in that prepared. Pig placenta extract for animal negative additives prepared according to the method of claim 1. After acidic hydrolysis of pig placenta for 5 hours at 110 ° C, the placenta hydrolysate was adjusted to pH 2 using NaOH, and the placental hydrolysate was mixed 1: 1 with an excipient to dry the moisture content below 10%. Method for producing a pig placenta extract for animal feed additives, characterized in that it is prepared by. Pig placenta extract for animal feed additives prepared according to the method of claim 3. Animal feed, characterized in that the pig placenta extract prepared according to claim 3 prepared by mixing 0.2 ~ 0.4% of the feed weight.

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