KR100678591B1 - Extraction method of Acanthopanax senticosus - Google Patents
Extraction method of Acanthopanax senticosus Download PDFInfo
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Abstract
본 발명은 오가피 추출 방법에 관한 것으로, 1-50메쉬(mesh)의 오가피 분말에 물질이동을 용이하게 하는 전처리를 수행한 후, 가압조건하에서 추출하여 유효성분을 추출하는 오가피 추출 방법을 제공하되, 상기 전처리 공정은 상기 오가피 분말에 증류수를 1g당 5-50㎖ 첨가하고, 그 혼합물에 마이크로파 혹은 초음파를 5-30분간 조사하거나, 상기 오가피 분말을 볶음장치에 넣고 150-250℃에서 5-30분간 로스팅하는 것에 의하여 오가피의 유효성분 추출률이 향상되도록 한 것이다.The present invention relates to a method for extracting scabies, after performing a pretreatment to facilitate mass transfer to 1-50 mesh (mesh) scabies powder, and providing an scabbard extraction method for extracting active ingredients by extracting under a pressurized condition. In the pretreatment step, 5-50 ml of distilled water per 1 g is added to the organo powder, and the mixture is irradiated with microwave or ultrasonic wave for 5-30 minutes, or the organo powder is placed in a roasting apparatus at 150-250 ° C. for 5-30 minutes. Roasting is to improve the extraction rate of the active ingredient of the organ.
오가피, 마이크로파, 초음파, 볶음, 열수추출 Ogapi, microwave, ultrasonic, stir, hot water extraction
Description
도 1은 본 발명의 전처리구와 대조구의 가용성 고형분 함량을 나타내는 도면1 is a view showing the soluble solids content of the pretreatment and control of the present invention
도 2는 본 발명의 전처리구와 대조구의 총당 함량을 나타내는 도면2 is a view showing the total sugar content of the pretreatment and control of the present invention
도 3은 본 발명의 전처리구와 대조구의 단백질 함량을 나타내는 도면Figure 3 is a diagram showing the protein content of the pretreatment and control of the present invention
도 4는 본 발명의 전처리구와 대조구의 폐놀성물질 함량을 나타내는 도면4 is a view showing the waste phenolic content of the pretreatment and control of the present invention
도 5는 본 발명의 전처리구와 대조구의 DPPH유리기 소거능을 나타내는 도면5 is a view showing the DPPH free radical scavenging ability of the pretreatment and control of the present invention
본 발명은 오가피에서 유효성분을 추출하기 위한 방법에 관한 것으로, 보다 상세하게는 오가피를 볶거나, 마이크로파 혹은 초음파를 조사하여 전처리함으로써 추출공정에서의 추출율이 향상되도록 하는 오가피 추출 방법에 관한 것이다.The present invention relates to a method for extracting an active ingredient from the scabies, and more particularly, to a scabbard extraction method for improving the extraction rate in the extraction process by roasting or pretreating microwaves or ultrasonic waves.
오가피는 전통적으로 근육과 골격을 강화시키고 사지마비, 하지, 무력감, 골절상, 타박상, 부종 등에 약효를 지니는 한약재로 널리 사용되어 왔으며 근래에 들어서는 과학적 연구를 통해 혈압강하작용, 간기능 보호능, 조혈촉진 및 면역기능 증진작용, 혈중콜레스테롤 저하작용, 항산화작용, 항알러지작용 및 항암작용 등을 가지는 것으로 규명되어 건강기능성 식품분야에서도 관심과 사용이 증가하는 추세 에 있다.Ogapi has traditionally been widely used as a medicinal herb for strengthening muscles and skeletons, and having medicinal effects on limb paralysis, lower limbs, weakness, fractures, bruises, and swelling.In recent years, scientific research has been used to lower blood pressure, protect liver function, and promote hematopoiesis. And it has been found to have an immune function enhancing action, blood cholesterol lowering action, antioxidant action, anti-allergic action and anti-cancer action, and the interest and use in the health functional food field is increasing.
약용식물체의 활용에 있어 가장 기본이 되는 처리공정은 재료로부터 유용성분을 분리하는 추출공정이며 주로 열수추출법이 사용되고 있으나, 추출에 장시간 소요되고 추출고형분의 수율이 낮은 단점이 있었다.The most basic treatment process for the utilization of medicinal plants is an extraction process for separating useful components from materials, and hot water extraction is mainly used, but it has a disadvantage in that the extraction takes a long time and the yield of extraction solids is low.
이러한 열수추출법의 문제점을 해결하기 위해 특수한 장치를 사용하는 마이크로파 추출(microwave-assisted extraction) 및 초임계유체 추출(supercritical fluid extraction) 그리고 초음파 세척기를 사용하는 초음파 추출(ultrasound-assisted extraction) 등의 방법이 연구되었으나 비경제적이라는 문제가 가장 크게 작용하여 상용화에 어려움이 있다.In order to solve the problem of hydrothermal extraction, methods such as microwave-assisted extraction and supercritical fluid extraction using special devices, and ultrasound-assisted extraction using an ultrasonic cleaner are provided. Although it is studied, the problem of non-economic is the biggest problem, which makes it difficult to commercialize.
상기한 바와 같은 문제점을 해결하기 위한 본 발명의 목적은 오가피를 볶거나, 마이크로파 혹은 초음파처리를 함으로써 추출공정에서의 추출률이 향상되도록 하는 데 있다.An object of the present invention for solving the problems as described above is to improve the extraction rate in the extraction process by roasting the organ, microwave or ultrasonic treatment.
상기 목적을 달성하기 위한 본 발명의 특징은 1-50메쉬(mesh)의 오가피 분말에 물질이동을 용이하게 하는 전처리를 수행한 후, 가압조건하에서 추출하여 유효성분을 추출하는 오가피 추출 방법을 제공하여 오가피의 유효성분 추출률이 향상되도록 한 것이다.A feature of the present invention for achieving the above object is to perform a pretreatment to facilitate mass transfer to 1-50 mesh (mesh) of the powder, and then to provide a method for extracting the extract to extract the active ingredient under pressure conditions It is to improve the extraction rate of the active ingredient of Ogapi.
상기한 구성에서 전처리 공정은 상기 오가피 분말에 증류수를 1g당 5-50㎖ 첨가하고, 그 혼합물에 마이크로파 혹은 초음파를 5-30분간 조사하거나, 상기 오가 피 분말을 볶음장치에 넣고 150-250℃에서 5-30분간 로스팅하는 것이다.In the above-described configuration, the pretreatment step is 5-50 ml of distilled water per 1 g of the organ powder, irradiated with microwave or ultrasonic wave for 5-30 minutes to the mixture, or put the organ powder in a stir fryer at 150-250 ° C Roast for 5-30 minutes.
상기한 특징을 갖는 본 발명의 실시예를 하기에서 첨부된 도면을 참조하여 보다 상세하게 살펴본다.An embodiment of the present invention having the above-mentioned features will be described in more detail with reference to the accompanying drawings.
본 발명에 따른 오가피 추출 방법은 8-20메쉬(mesh)의 오가피 분말에 물질이동을 용이하게 하는 전처리를 수행하는 전처리공정과, 상기 전처리공정을 완료한 후 가압조건하에서 열수추출하여 유효성분을 추출하는 가압추출공정으로 구성된다.Extraction method according to the present invention is a pre-treatment step to perform a pre-treatment to facilitate the movement of material to 8-20 mesh (o) mesh powder, and extract the active ingredient by extracting hot water under pressurized conditions after completing the pretreatment step It consists of a pressure extraction process.
상기한 구성에서 오가피(Acanthopanax senticosus)는 대구약령시장에서 구입하여, 실험에 사용하기 위해 분쇄기로 분쇄하고 표준체(8, 20 mesh)로 체질한 다음 플라스틱 필름봉지로 밀봉하여 4℃에서 보관한다.In the above configuration, Acanthopanax senticosus is purchased at Daegu Yangnyeong Market, crushed with a grinder for use in experiments, sieved with standard bodies (8, 20 mesh), sealed with a plastic film bag, and stored at 4 ° C.
그리고 가압추출공정은 오토클레이브(autoclave)를 사용하여 100-200℃(본 발명의 실시예에서는 121℃)에서 1-3시간(본 발명의 실시예에서는 2시간) 실시한다.And the pressure extraction process is carried out for 1 to 3 hours (2 hours in the embodiment of the present invention) at 100-200 ℃ (121 ℃ in the embodiment of the present invention) using an autoclave (autoclave).
한편, 상기 전처리 공정을 하기에서 각 실시예별로 살펴본다.Meanwhile, the pretreatment process will be described for each embodiment below.
실시예 1Example 1
8-20메쉬의 오가피 분말 10g에 증류수 200㎖를 첨가하고 그 혼합물을 전자레인지에 넣고 2,450 MHz의 마이크로파로 10분간 조사하여 전처리 공정을 수행한다.To 10 g of 8-20 mesh organ powder, 200 ml of distilled water is added, and the mixture is placed in a microwave oven and irradiated with microwave at 2,450 MHz for 10 minutes to perform a pretreatment process.
실시예 2Example 2
시료 분말(8~20 mesh) 10g에 증류수 200㎖를 첨가한 혼합물을 초음파 파쇄기(Sonic Dismembrator F550, Fisheer Scientific, USA)의 20 kHz로 10분간 전처리공 정을 수행한다.The mixture obtained by adding 200 ml of distilled water to 10 g of sample powder (8-20 mesh) is pretreated for 10 minutes at 20 kHz using an ultrasonic crusher (Sonic Dismembrator F550, Fisheer Scientific, USA).
실시예 3Example 3
시료 분말(8~20 mesh) 10g을 볶음장치에 넣고 180℃에서 10분간 수행한다.10g of the sample powder (8 ~ 20 mesh) is put into the roasting apparatus and performed at 180 ° C for 10 minutes.
한편, 상기 전처리공정에서 볶음 처리는 고온에 기인된 성분과 조직의 변화를 유발하여 추출수율과 풍미의 향상을 유발하고, 마이크로파와 볶음 처리는 물질이동을 용이하게 하는 조직의 가열연화를 유도하며, 초음파 처리는 물질전달 통로를 형성하는 성질로 인해 구성성분의 열수추출의 효율을 개선시키며, 이러한 전처리 공정의 효과를 하기에서 대조구와의 비교를 통해서 보다 상세하게 살펴본다.On the other hand, in the pretreatment process, the roasting process causes changes in the components and tissues due to the high temperature, which leads to an improvement in extraction yield and flavor, and the microwave and roasting process induces heat softening of the tissue to facilitate material movement. The sonication improves the efficiency of hot water extraction of the constituents due to the nature of forming the material transfer passage, and the effect of this pretreatment process will be discussed in more detail by comparison with the control.
상기한 바와 같은 공정을 통한 추출효율을 확인하기 위하여. 대조구로 무처리 오가피 분말을 사용하고, 추출은 전처리공정을 수행한 본 발명의 전처리구와 대조구 각각 10g을 증류수 200㎖로 정용하고 히팅맨틀(heating mantle)을 사용한 상압(100℃)과 오토클레이브(autoclave)를 사용한 가압(121℃) 조건하에서 각각 2시간씩 실시한다. In order to confirm the extraction efficiency through the process as described above. As a control, an untreated organ powder was used, and the extraction was performed with a pretreatment of the present invention and a control which were subjected to a pretreatment process, respectively, 10 g of distilled water with 200 ml of distilled water, and an atmospheric pressure (100 ° C.) and an autoclave using a heating mantle. 2 hours are performed under pressurized (121 degreeC) conditions using ().
이와 같이 전처리구와 대조구의 결과를 하기에서 비교하여 살펴보되, 실험결과의 통계처리는 SPSS software(ver. 12, SPSS Inc., USA)를 이용하여 분산분석과 Duncan's multiple range test(α=0.05)를 실시한다.As described above, the results of the pretreatment and the control were compared, and the statistical results of the experimental results were analyzed by ANOVA and Duncan's multiple range test (α = 0.05) using SPSS software (ver. 12, SPSS Inc., USA). Conduct.
가용성 고형분 측정Soluble solids measurement
가용성 고형분의 함량은 각 조건으로 추출한 액 일부를 취해 굴절계(N-1E, Atago, Japan)를 사용하여 측정하였다.The content of soluble solids was measured using a refractometer (N-1E, Atago, Japan) taking a portion of the liquid extracted under each condition.
본 발명의 오가피 열수 추출액의 가용성 고형분 농도를 측정한 결과는 도 1에 나타내는 바와 같이 가용성 고형분의 함량은 상압과 가압추출액 모두에서 대조구에 비해 전처리구에서 높았으며, 동일한 전처리구일 경우는 상압추출구보다는 가압추출구에서 높은 가용성 고형물 함량을 나타내었다.As a result of measuring the concentration of soluble solids of the extract of the hot-water extract of the present invention, as shown in FIG. 1, the content of the soluble solids was higher in the pretreatment than the control in both the atmospheric pressure and the pressurized extract. It showed high soluble solids content in the pressure extraction port.
이러한 결과를 볼 때, 오가피로부터 총괄적인 수용성 성분의 추출은 추출방법에 영향은 받지만 추출 전 초음파, 마이크로파 및 볶음 처리에 의해 촉진되므로, 추출시간을 단축시킬 수 있게 된다.In view of these results, the extraction of the overall water-soluble component from the ogapi is influenced by the extraction method, but is accelerated by the ultrasonic, microwave and roasting process before extraction, it is possible to shorten the extraction time.
총당 측정Total sugar
총당 함량은 phenol-sulfuric acid법에 따라 측정하였다. 즉, 추출액 1㎖에 5% 페놀1㎖와 황산5㎖를 가하여 발색시킨 다음 20분간 방치 후 spectrophotometer(UV1601, Shimadzu, Japan)를 이용하여 470nm에서 흡광도를 측정하였다. 총당의 정량은 glucose 표준품을 사용하여 검량선을 작성하여 실시하고, 총당의 추출수율은 오가피 시료무게에 대한 추출된 총당의 무게로 나타내었다.Total sugar content was measured by phenol-sulfuric acid method. That is, 1 ml of 5% phenol and 5 ml of sulfuric acid were added to the extract, and the resultant was allowed to stand for 20 minutes and the absorbance was measured at 470 nm using a spectrophotometer (UV1601, Shimadzu, Japan). The total sugar was quantitatively prepared by using a calibration standard using glucose standard, and the extraction yield of total sugar was expressed as the weight of extracted total sugar to the weight of Ogapi sample.
전처리 및 추출 방법에 따른 오가피로부터 총당의 추출수율을 측정한 결과는 도 2에 나타내는 바와 같이 추출방법에 상관없이 초음파와 마이크로파 전처리구에서는 대조구보다 높았으나 볶음처리구는 대조구와 유의적인 차이를 보이지 않았다.As a result of measuring the extraction yield of the total sugar from the agapi according to the pre-treatment and extraction method, as shown in Figure 2, the ultrasonic and microwave pre-treatment was higher than the control, regardless of the extraction method, but the roasting treatment did not show a significant difference from the control.
그리고, 각 전처리구에서는 상압추출보다는 가압추출의 경우가 약 25% 높은 총당의 추출수율을 보였다.In addition, in each pretreatment, the extraction yield of total sugar was about 25% higher than that of normal extraction.
따라서, 오가피의 열수추출에 있어 초음파와 마이크로파 전처리는 총당의 추출수율을 증대시키는 효과가 있으며 여기에 가압추출이 병행되면 추출수율은 더욱 증가됨을 알 수 있다.Therefore, the ultrasonic and microwave pretreatment in the extraction of hot water of Ogapi has the effect of increasing the extraction yield of total sugar, and it can be seen that the extraction yield is further increased when the extraction is combined with pressure.
단백질 측정Protein measurement
단백질 함량은 Lowry법에 따라 측정하였다. 즉, 추출액 0.1㎖에 2N NaOH 0.1㎖를 가하고 10분간 가열한 후 상온으로 냉각시키고 여기에 complex-forming reagent 1㎖를 가하고 10분간 방치 후 Folin reagent 0.1㎖를 넣고 혼합 후 30분간 방치하고 spectrophotometer(UV1601, Shimadzu, Japan)를 이용하여 750 nm에서 흡광도를 측정하였다. 단백질의 정량은 bovine serum albumin 표준품을 사용하여 검량선을 작성하여 실시하고, 단백질의 추출수율은 오가피 시료무게에 대한 추출된 단백질의 무게로 나타내었다.Protein content was measured according to the Lowry method. That is, 0.1 ml of 2N NaOH was added to 0.1 ml of the extract, heated for 10 minutes, cooled to room temperature, 1 ml of a complex-forming reagent was added thereto, and left for 10 minutes, 0.1 ml of Folin reagent was added thereto, mixed, and allowed to stand for 30 minutes, followed by spectrophotometer (UV1601). , Shimadzu, Japan) was used to measure the absorbance at 750 nm. Protein quantification was performed by preparing a calibration curve using bovine serum albumin standard, and the extraction yield of protein was expressed as the weight of the extracted protein to the weight of the sample.
전처리 및 추출 방법에 따른 오가피로부터 단백질의 추출수율을 측정한 결과는 도 3에 나타낸다. 단백질의 열수 추출수율은 상압추출구에서는 볶음처리구만이 다른 방법의 전처리구보다 유의적으로 높게 나타내었으나 가압추출구에서는 대조구보다 초음파와 마이크로파 처리구가 약간 높은 추출수율을 나타낸 반면 볶음처리구는 상당히 높은 수율을 나타내었다.The result of measuring the extraction yield of the protein from the shreds according to the pretreatment and extraction method is shown in FIG. 3. The extraction yield of hot water of protein was significantly higher in the extracts than in the other methods, but the extraction and extraction of the hot water were slightly higher than those of the control. Indicated.
즉, 동일한 전처리 조건일 경우는 상압보다는 가압추출에서 유의적으로 높은 단백질 추출수율을 나타내고, 오가피의 열수 추출에 있어 단백질의 추출수율은 추출온도의 영향이 적으며 볶음 전처리에 의해서 더욱 증가된다.That is, under the same pretreatment conditions, the protein extraction yield was significantly higher than that at normal pressure, and the extraction yield of protein in the extraction of hot water of Ogapi was less affected by the extraction temperature and was further increased by the roasting pretreatment.
페놀성물질 측정Phenolic substance measurement
총페놀성 물질의 함량은 Folin-Denis법에 따라 측정한다. 즉, 추출액 5㎖를 취하여 Folin-Denis reagent 5㎖를 가하고 3분간 정치한 다음 10% Na2CO3 용액 5㎖를 가한다. 이 혼합액을 1시간 동안 정치한 후 spectrophotometer(UV1601, Shimadzu, Japan)를 이용하여 760nm에서 흡광도를 측정하고, gallic acid 표준품으로 검량선을 작성하여 정량한다. 페놀성 물질의 추출수율은 오가피 시료무게에 대한 추출된 단백질의 무게로 나타낸다.The total phenolic content is determined according to the Folin-Denis method. In other words, take 5 ml of the extract, add 5 ml of Folin-Denis reagent, allow to stand for 3 minutes, and then add 5 ml of 10% Na 2 CO 3 solution. After allowing the mixture to stand for 1 hour, absorbance at 760 nm is measured using a spectrophotometer (UV1601, Shimadzu, Japan), and a calibration curve is prepared using a gallic acid standard. The extraction yield of phenolic material is expressed as the weight of the extracted protein to the weight of the sample.
전처리 및 추출 방법에 따른 오가피로부터 페놀성 물질의 추출수율을 측정한 결과는 도 4에 나타낸다. 페놀성 물질의 열수 추출수율은 전반적으로 상압 추출구보다는 가압 추출구에서 높게 나타났다. 전처리 방법의 영향을 보면, 페놀성 물질의 추출수율이 상압추출구에서는 대조구보다 초음파와 마이크로파 처리구에서 높게 나타났으나 가압추출구에서는 볶음처리구 역시 초음파와 마이크로파 처리구와 더불어 대조구보다 높게 나타났다.The result of measuring the extraction yield of the phenolic substance from Ogapi according to the pretreatment and extraction method is shown in FIG. The yield of hot water extraction of phenolic compounds was higher in the pressure extraction section than in the pressure extraction section. In the effect of pretreatment method, the extraction yield of phenolic substance was higher in the ultrasonic and microwave treatments than in the control, but the roasting treatment was also higher than the control in the pressure extraction.
이로써 오가피의 열수 추출에 있어 페놀성 물질의 추출수율은 추출조건에 따라 약간의 차이는 있지만 추출 전 초음파, 마이크로파 및 볶음 처리는 추출수율 증대에 유효한 것으로 판단된다.As a result, the extraction yield of phenolic substances in the extraction of hot water of Ogapi was slightly different depending on the extraction conditions, but the ultrasonic, microwave and roasting treatments before extraction were considered effective for increasing the extraction yield.
DPPH유리기 소거능 측정DPPH free radical scavenging capacity measurement
Diphenyl picryl hydrazyl(DPPH) radical radical scavenging activity는 Blois의 방법으로 측정한다. 즉, 추출액 0.2㎖에 에탄올에 용해한 4×10-4M DPPH 용액 0.8㎖에 에탄올 2.8㎖을 혼합한 것을 가하고 10초간 강하게 진탕하고 10분간 정치한 후에 spectrophotometer(UV1601, Shimadzu, Japan)를 이용하여 525nm에서 흡광도를 측정한다. 소거활성은 다음 식으로 계산한다.Diphenyl picryl hydrazyl (DPPH) radical radical scavenging activity is measured by Blois method. That is, 0.2 ml of the extract was mixed with 0.8 ml of 4 × 10-4M DPPH solution dissolved in ethanol, and 2.8 ml of ethanol. After 10 minutes of vigorous shaking, the mixture was left to stand for 10 minutes, followed by spectrophotometer (UV1601, Shimadzu, Japan) at 525 nm. Measure the absorbance. Scavenging activity is calculated by the following equation.
전처리 및 추출 방법에 따른 오가피 추출액의 DPPH radical scavenging activity를 측정한 결과는 도 5에 나타낸다. 추출액의 DPPH radical scavenging activity는 상압 및 가압 추출조건에서 대조구보다 초음파와 마이크로파 처리구에서 약간 높게 나타난 반면에 볶음처리구에서는 상당히 높은 값을 나타내었다. 이러한 결과를 볼 때, 오가피의 열수 추출액의 DPPH radical scavenging activity는 초음파, 마이크로파 및 볶음 전처리에 의해 높아지지만 특히, 볶음 처리에 의해 더욱 높아지는 것으로 판단된다. DPPH radical scavenging activity는 항산화력을 나타내는 지표로 사용하며 일반적으로 페놀성 물질의 함량과 연관성이 아주 높은 것으로 알려져 있다.The results of measuring DPPH radical scavenging activity of the extract of Ogapi extract according to the pretreatment and extraction method are shown in FIG. 5. The DPPH radical scavenging activity of the extract was slightly higher in the ultrasonic and microwave treatments than in the control under normal and pressurized extraction conditions, while in the roasting treatments. From these results, DPPH radical scavenging activity of the hot water extract of Ogapi was increased by ultrasonic wave, microwave and roasting pretreatment, but especially by roasting process. DPPH radical scavenging activity is used as an indicator of antioxidant activity and is generally known to be highly related to the content of phenolic substances.
그러나 도 4에 나타내는 바와 같이 볶음처리구의 페놀성 물질의 함량이 다른 전처리구보다 높지 않음에도 불구하고 DPPH radical scavenging activity는 높게 나타난 것은 볶음처리에 의해 항산화력이 강한 물질이 생성되었기 때문이다.However, as shown in FIG. 4, the DPPH radical scavenging activity was high even though the content of the phenolic substance of the roasting treatment was not higher than that of the other pretreatment, because the strong antioxidant power was produced by the roasting treatment.
따라서, 상기한 실험결과에 나타내는 바와 같이 오가피의 열수 추출에 있어 초음파, 마이크로파 및 볶음 전처리와 가압추출을 시행하는 것은 영양 및 생리활성 성분의 추출수율을 대폭 증대시킨다.Therefore, as shown in the above experimental results, the ultrasonic extraction, microwave and roasting pretreatment and pressurization extraction in hot water extraction of organopi significantly increase the extraction yield of nutrient and bioactive components.
본 발명에 따른 오가피 추출 방법에 의하면 비교적 범용성을 가진 장비들 즉 , 전자오븐, 초음파 파쇄기, 볶음장치를 이용하여 유용성 성분의 추출효율을 향상시킬 수 있게 되므로, 유용성 성분의 추출을 위한 장비의 채택에 어려움이 발생되지 않는다.According to the ogapi extraction method according to the present invention can improve the extraction efficiency of the useful component by using a relatively general equipment, that is, an electronic oven, an ultrasonic crusher, a roasting device, the adoption of equipment for the extraction of the useful component Difficulties do not arise.
또한, 본 발명에 따른 전처리는 성분들의 추출수율과 유리기 소거능을 증가 시키는 경향을 보이며 특히, 가용성 고형물, 총당 및 페놀성 물질의 추출수율은 마이크로파와 초음파에 의해, 단백질의 추출수율과 유리기 소거능은 볶음처리에 의해 더욱 증가된다.In addition, the pretreatment according to the present invention shows a tendency to increase the extraction yield and free radical scavenging ability of the components, in particular, the extraction yield of soluble solids, total sugars and phenolic substances by microwave and ultrasonic, the extraction yield and free radical scavenging ability of the protein is stir-fry Further increased by treatment.
그리고, 상압추출보다 가압추출에 의해 가용성 고형물, 총당, 단백질 및 페놀성 물질의 추출수율이 증대된다.In addition, the extraction yield of soluble solids, total sugars, proteins and phenolic substances is increased by pressure extraction rather than atmospheric extraction.
따라서, 본 발명에 의하면 오가피의 유효성분을 추출함에 있어서 시간이 대폭 감소되고, 그 추출율이 대폭 향상되는 효과가 있다.Therefore, according to the present invention, there is an effect that the time is greatly reduced in extracting the active ingredient of Ogapi, and the extraction rate is greatly improved.
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| KR101548165B1 (en) * | 2013-08-07 | 2015-08-31 | 이한신 | Method for producing Acanthopanax leaves extract with the enhanced taste and flavor and Acanthopanax leaves extract produced by the same method |
| KR101556561B1 (en) * | 2013-12-26 | 2015-10-01 | 장흥군 | Method for producing Chungtaejeon extract and Chungtaejeon extract produced by same method |
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| KR101980449B1 (en) * | 2017-11-06 | 2019-05-20 | 임경하 | Method for preparing pine nut with an improved taste and flavor |
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