KR100647085B1 - The production method of taurine enriched pork by supplementation of feather meal and thereof the pork - Google Patents

The production method of taurine enriched pork by supplementation of feather meal and thereof the pork Download PDF

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KR100647085B1
KR100647085B1 KR1020050013453A KR20050013453A KR100647085B1 KR 100647085 B1 KR100647085 B1 KR 100647085B1 KR 1020050013453 A KR1020050013453 A KR 1020050013453A KR 20050013453 A KR20050013453 A KR 20050013453A KR 100647085 B1 KR100647085 B1 KR 100647085B1
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pork
taurine
feed
pyridoxine
test
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백인기
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제일사료 주식회사
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/08Radiation
    • A61L2/10Ultraviolet radiation
    • AHUMAN NECESSITIES
    • A47FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
    • A47LDOMESTIC WASHING OR CLEANING; SUCTION CLEANERS IN GENERAL
    • A47L5/00Structural features of suction cleaners
    • A47L5/12Structural features of suction cleaners with power-driven air-pumps or air-compressors, e.g. driven by motor vehicle engine vacuum
    • A47L5/22Structural features of suction cleaners with power-driven air-pumps or air-compressors, e.g. driven by motor vehicle engine vacuum with rotary fans
    • A47L5/24Hand-supported suction cleaners
    • A47L5/26Hand-supported suction cleaners with driven dust-loosening tools
    • AHUMAN NECESSITIES
    • A47FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
    • A47LDOMESTIC WASHING OR CLEANING; SUCTION CLEANERS IN GENERAL
    • A47L9/00Details or accessories of suction cleaners, e.g. mechanical means for controlling the suction or for effecting pulsating action; Storing devices specially adapted to suction cleaners or parts thereof; Carrying-vehicles specially adapted for suction cleaners
    • A47L9/10Filters; Dust separators; Dust removal; Automatic exchange of filters
    • A47L9/102Dust separators
    • A47L9/104Means for intercepting small objects
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A61L2/0023Heat
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/10Apparatus features
    • A61L2209/14Filtering means

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Biomedical Technology (AREA)
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  • Chemical & Material Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

본 발명은 우모분을 이용한 타우린 함량이 증가된 돈육의 생산방법 및 상기 방법으로 생산된 돈육에 관한 것으로, 보다 상세하게는 우모분 및 피리독신을 첨가한 사료를 급여하여 타우린 함량이 증가된 돈육의 생산방법 및 상기 방법에 의해 생산된 돈육에 관한 것이다.The present invention relates to a method of producing pork with increased taurine content using cow flour and pork produced by the above method, and more particularly, to feed a feed containing cow flour and pyridoxine to produce pork with increased taurine content. The method and the pork produced by the method.

본 발명에 의하면, 가금육의 소비 후 폐기처리되는 우모를 사료로 재활용할 수 있고, 타우린 함량이 증가된 기능성 돈육을 생산하도록 함으로써 타우린 섭취가 부족한 사람들에게 타우린을 간편하게 공급해 줄 수 있다.According to the present invention, it is possible to recycle the feathers disposed of after the consumption of poultry meat as feed, and to provide taurine to those who lack taurine by producing functional pork with increased taurine content.

우모분, 피리독신, 타우린, 돈육, 사료 Dairy powder, pyridoxine, taurine, pork, fodder

Description

우모분을 이용한 타우린 강화 돈육의 생산방법 및 상기 방법으로 생산된 돈육{THE PRODUCTION METHOD OF TAURINE ENRICHED PORK BY SUPPLEMENTATION OF FEATHER MEAL AND THEREOF THE PORK }The production method of taurine fortified pork using woowoo powder and pork produced by the above method {THE PRODUCTION METHOD OF TAURINE ENRICHED PORK BY SUPPLEMENTATION OF FEATHER MEAL AND THEREOF THE PORK}

본 발명은 우모분을 이용한 타우린 함량이 증가된 돈육의 생산방법 및 상기 방법으로 생산된 돈육에 관한 것이다.The present invention relates to a production method of pork with an increased taurine content using a feather powder and pork produced by the above method.

타우린(taurine)(2-amino-ethanesulfonic acid)은 포유동물의 체내에서 생합성되는 함황아미노산(sulfonic amino acid)의 하나로서 세포 내액에서 유리된 상태로 존재하고(Huxtable R.J., Physiol. Rev., 72, pp101-163, 1992; Worden J.A. and Stipanuk M.H., Comp. Biochem. Physiol., 82B2, pp233-239, 1985), 특히 뇌, 안구, 근육, 간 등의 골격근과 신경계의 유리 아미노산 집단에서 높은 농도로 발견되며, 해독과 항산화 작용을 통해 간 기능 강화와 혈중 콜레스테롤 수치 감소 및 혈압 조절 등 다양한 기능성을 가진 건강 소재로 오래전부터 식품 및 음료에 사용되어 왔다. 타우린은 육식동물 체내에서 담즙산을 포함하여 타우로콜산(taurocholic acid)의 형태로 담즙을 통해 배출되는데, 담즙으로 배출된 타우린은 계면활성 작용에 의해 장내에서 지방 또는 지용성 비타민의 흡수를 촉진시키고, 또 한 이담작용에 의해 급성 간염시 황달의 경감작용과 고지혈증 환자의 혈청 내 중성지방과 콜레스테롤의 수치를 저하시키는 효과가 있으며, 이외에도 뇌의 발육, 망막의 광수용체 활성, 생식 및 정상적인 성장 발달, 항산화 활성, 신경 전달 등 다양한 기능을 가지고 있는 것으로 보고되고 있다(Jacobsen et al., Physiol. Rev., 48, pp424-511, 1968). 이와 같은 생리활성으로 인해 타우린은 유아용 이유식이나 껌, 음료 및 자양강장 드링크제 등의 각종 건강식품이나 의약품의 첨가물로 사용되고 있으나 약물로서는 자주 사용되지 않으며, 치료적 사용은 심장질환의 치료에 대해서만 보고되고 있다.Taurine (2-amino-ethanesulfonic acid) is one of the sulfuric acid amino acids that are biosynthesized in the body of mammals and exists freely in intracellular fluids (Huxtable RJ, Physiol. Rev., 72 , pp101-163, 1992; Worden JA and Stipanuk MH, Comp. Biochem.Physiol . , 82B2 , pp233-239, 1985), found in high concentrations in free amino acid populations of skeletal muscle and nervous system, especially brain, eye, muscle and liver It has been used in food and beverages for a long time as a healthy material with various functions such as strengthening liver function, reducing blood cholesterol level and controlling blood pressure through detoxification and antioxidant activity. Taurine is excreted through bile in the form of taurocholic acid, including bile acids, in the carnivorous body. Taurine, which is released as bile, promotes the absorption of fat or fat-soluble vitamins in the intestine by interfacial action. A bile biliary effect reduces jaundice during acute hepatitis and lowers serum triglyceride and cholesterol levels in hyperlipidemic patients.In addition, brain development, retinal photoreceptor activity, reproductive and normal growth development, and antioxidant activity , Neurotransmission, etc., has been reported (Jacobsen et al., Physiol. Rev. , 48 , pp424-511, 1968). Due to such physiological activity, taurine is used as an additive to various health foods and medicines such as baby food, gum, beverages, and nutrient tonic drinks, but it is not frequently used as a drug, and therapeutic use is reported only for the treatment of heart disease. .

포유류의 조직에서 타우린은 함황 아미노산인 시스테인(cysteine)과 메티오닌(methionine)으로부터 생합성되는데, 인체의 경우 타우린의 생합성에 관여하는 시스테인 다이옥시게나제(cysteine dioxygenase)와 시스테인 디카르복실라제(cysteine decarboxylase)의 활성이 매우 낮아 타우린의 생합성이 거의 이루어지지 않고 있으며, 따라서 외부로부터 타우린을 공급받아야 한다(Vinton et al., Pediatr. Res., 21, pp399-403, 1987). In mammalian tissues, taurine is biosynthesized from the sulfur-containing amino acids cysteine and methionine. In the human body, taurine is synthesized from cysteine dioxygenase and cysteine decarboxylase, which are involved in the biosynthesis of taurine. The activity is so low that biosynthesis of taurine is hardly achieved, and therefore, taurine must be supplied from the outside (Vinton et al., Pediatr. Res. , 21 , pp 399-403, 1987).

그러나, 타우린을 직접 제품에 추가하거나 타우린을 풍부하게 함유하고 있는 사료를 직접 공급하여 타우린을 다량 함유하는 식품 등의 제품을 생산하는 방법에는 다음과 같은 문제점이 있다. However, there are the following problems in the method of producing products such as foods containing a large amount of taurine by directly adding taurine to the product or by directly supplying a feed containing abundant taurine.

먼저 합성 타우린을 직접 사료에 첨가하는 경우는 첨가수준과 돈육으로의 전이율을 감안할 때 경제성이 떨어질 수 있으며, 합성타우린 첨가로 생산된 돈육은 체내에서 자연적으로 합성된 타우린이 강화된 돈육에 비해 소비자의 기호도가 떨어 진다. 또한 타우린을 풍부하게 함유하고 있는 오징어, 오징어 내장, 조개류 등을 사료로 직접 공급하는 것은 실용적으로나 경제적으로 비현실적이다. First, when synthetic taurine is added directly to the feed, it may be less economical in view of the level of addition and the transfer rate to pork, and the pork produced by the addition of synthetic taurine is more consumer than the taurine-enriched pork that is naturally synthesized in the body. The degree of acceptability decreases. In addition, it is practically and economically impractical to feed squid, squid guts, and shellfish, which are rich in taurine, directly.

한편, 우모분은 가금의 깃털(우모)을 고압 하에서 가열처리하여 건조, 분쇄한 것으로 타우린의 전구물질인 시스틴(cystine)의 함량이 4.34%(NRC, 1994)로 가축사료 원료 중 가장 높다. 현재까지 알려진 타우린의 생합성 경로는, 시스테인 설피네이트 (cysteinesulfinate)경로와 시스타민(cysteamine) 경로 두 가지가 보고 되었다. 대부분의 조직에서 타우린 생합성의 주된 경로로서 시스테인 설피네이트(cysteine sulfinate, CSA)는 시스테인 디옥시제네이즈(cysteine dioxygenase, CD)의 작용에 의해 시스테인(cysteine)이 CSA로 산화된 후 다시 시스테인 설피네이트 디카복실레이즈 (cysteine sulfinate decarboxylase, CSAD)의 작용에 의해 하이포타우린 (hypotaurine)으로 전환되고 시스타민(cysteamine)은 효소의 작용 없이 타우린(taurine)으로 전환 되어진다. 위 효소들은 함황아미노산 대사에 중요한 시스타치온 합성효소(cystathione synthase), 시스타치온네이즈 (cystathionase), 시스테인 디옥시제네이즈(cysteine dioxygenase)와 함께 피리독신 (pyridoxine Pyridoxine-5-phosphate)의 조효소 작용에 크게 의존한다. On the other hand, feather powder is dried and pulverized by heating the feathers (feathers) under high pressure. The content of cystine, a precursor of taurine, is 4.34% (NRC, 1994), which is the highest among livestock feed. Two known biosynthetic pathways of taurine have been reported, the cysteinesulfinate pathway and the cysteamine pathway. Cysteine sulfinate (CSA) is a major pathway of taurine biosynthesis in most tissues. By the action of cysteine sulfinate decarboxylase (CSAD) it is converted to hypotaurine and cysteamine is converted to taurine without the action of enzymes. These enzymes, together with cystathione synthase, cystathionase and cysteine dioxygenase, are important for the coenzyme action of pyridoxine Pyridoxine-5-phosphate Depends heavily

현재까지 타우린 강화 축산물 개발이 시도되었으나 타우린의 생합성 경로를 이용하여 우모분과 피리독신을 첨가함으로써 자연적으로 돈육 내 타우린을 강화시키는 방법에 대한 연구 사례는 전무한 실정이다.To date, there have been attempts to develop taurine-enriched livestock products, but there are no studies on how to strengthen taurine naturally in pork by adding woowoo powder and pyridoxine using taurine biosynthetic pathway.

상기와 같은 문제점을 해결하기 위한 본 발명의 목적은 돈육에 타우린함량을 증가시키기 위하여 타우린의 전구체인 시스틴(cystine)을 함유한 우모분을 함유한 사료를 급여하여 생산된 타우린 함량이 증가된 돈육의 생산방법을 제공하고자 한다.An object of the present invention for solving the above problems is to increase the taurine content of pork in order to increase the taurine content produced by feeding a feed containing a cow's powder containing cystine (cystine) precursor of taurine To provide a production method.

또한 본 발명의 목적은 상기 방법으로 생산된 타우린 함량이 증가된 돈육을 제공하고자 한다.It is also an object of the present invention to provide a pork with increased taurine content produced by the above method.

상기와 같은 기술적 과제를 달성하기 위한 본 발명은 우모분을 함유한 사료로 사양된 것을 특징으로 하는 타우린 함량이 증가된 돈육의 생산방법을 제공한다. The present invention for achieving the above technical problem provides a production method of pork with increased taurine content, characterized in that it is specified as feed containing feather powder.

또한, 본 발명은 상기 방법으로 생산된 타우린 함량이 증가된 돈육을 제공한다.The present invention also provides pork with increased taurine content produced by the above method.

이하 본 발명의 구성을 상세히 설명하기로 한다.Hereinafter, the configuration of the present invention will be described in detail.

본 발명은 우모분을 함유한 사료로 사양된 것을 특징으로 하는 타우린 함량이 증가된 돈육의 생산방법을 제공한다. 이때, 상기 사료는 급여되는 사료 전체 중량대비 1 ~ 10 중량%의 우모분을 함유한 사료인 것이 바람직하며, 여기에 5 ~ 20 ppm의 피리독신을 더 첨가한 사료인 것이 바람직하다. 10 중량%를 초과하여 우모분을 첨가하면 소화이용율이 떨어지는 문제가 있으며, 피리독신은 우모분에 함유된 타우린 합성의 전구체인 시스틴(cystine)의 전환에 필요한 효소들의 작용에 조효소로 작용하므로 첨가되는 우모분 함량에 따라 조절될 수 있으나, 5 ~ 20 ppm이 바람직하며, 10 ppm인 것이 가장 바람직하다.The present invention provides a method for producing pork with increased taurine content, characterized in that it is specified as feed containing cow's powder. At this time, the feed is preferably a feed containing 1 to 10% by weight of the feather content relative to the total weight of the feed, it is preferable that the feed is added to 5 to 20 ppm of pyridoxine. Digestion utilization is lowered when more than 10% by weight of feather powder is added, and pyridoxine acts as a coenzyme for the action of enzymes required for the conversion of cystine, a precursor of taurine synthesis, which is contained in feather powder. Although it can be adjusted according to the powder content, 5 to 20 ppm is preferable, and 10 ppm is most preferable.

또한, 본 발명은 상기 방법으로 생산된 타우린 함량이 증가된 돈육을 제공한 다.In addition, the present invention provides pork with increased taurine content produced by the above method.

구체적으로, 본 발명자는 타우린 합성의 전구물질인 시스틴(cystine)공급원으로서 우모분과 피리독신을 각각 기초사료에 첨가하여 육성 비육돈의 육성기(개시일 ~ 3주), 육성-비육기(3 ~ 8주) 및 비육기(8 ~ 11주) 각각에 대한 일당 증체량, 사료섭취량, 사료요구율, 폐사율과 등심, 안심, 햄, 심장 및 간에서의 타우린(taurine)함량에 미치는 영향을 시험한다. 이때 상기 사료는 기초사료에 우모분을 사료 전체 중량대비 3 중량% 및 6 중량% 단독으로 첨가한 시험구 및 상기에 각각 피리독신 10 ppm을 추가로 첨가한 시험구를 제조하여 그 영향을 비교한다. 본 시험에서 각 시험구 중에서 6 중량%의 우모분과 10ppm의 피리독신이 첨가된 사료로 사양된 돈육 내 타우린 강화 효과가 가장 우수하며, 특히 심장, 안심, 햄 및 등심의 경우 기초사료만으로 사양된 돈육에 비해 타우린 함량이 각각 91%, 23%, 27% 및 29% 증가하는 효과를 나타낸다.Specifically, the present inventors add woowoo powder and pyridoxine as a source of cystine, a precursor of taurine synthesis, to the basic feed, respectively, for the rearing period (starting to 3 weeks), the rearing period (3 to 8 weeks), and The daily weight gain, feed intake, feed demand, mortality and mortality, tenderloin, ham, heart, and taurine content in the finishing period (8-11 weeks) are tested. At this time, the feed is prepared by the test feed with the addition of 3% by weight and 6% by weight relative to the total weight of the feed and the base and the addition of 10 ppm of pyridoxine, respectively, to compare the effect. In this test, the taurine fortification effect in pork, which was fed with 6% by weight of feather powder and 10ppm of pyridoxine, was the best in each test, especially in the heart, tenderloin, ham and sirloin. In comparison, the taurine content is increased by 91%, 23%, 27% and 29%, respectively.

또한 본 발명자는 상기 우모분과 피리독신을 함유한 사료로 사양한 돈육의 등심과 삼겹살 샘플을 채취하여 신선육의 경우, 향미, 육색, 불쾌취, 육즙의 삼출정도, 전반적인 기호도를 평가하고, 조리육의 경우, 향미, 풍미, 다즙성, 육질의 부드러운 정도, 전반적인 기호도를 평가하였다. In addition, the present inventors collected the sirloin and pork belly sample of the pork specification as feed containing the feather powder and pyridoxine to evaluate the flavor, meat color, unpleasantness, effusion of juice, overall palatability in the case of fresh meat, and cooked meat. , Flavor, flavor, juiciness, tenderness of meat, and overall palatability were evaluated.

이하 본 발명의 구성을 실시예, 시험예, 도면 및 도표를 통하여 상세히 설명하기로 한다. 다만, 본 발명의 권리범위는 실시예, 시험예, 도면 및 도표에 의하여 본 발명의 청구범위가 한정되는 것은 아니다.Hereinafter, the configuration of the present invention will be described in detail through examples, test examples, drawings and diagrams. However, the scope of the present invention is not limited to the claims of the present invention by examples, test examples, drawings and diagrams.

실시예 1. 시험사료의 준비Example 1 Preparation of Test Feed

본 시험에 사용된 기초사료의 배합비와 성분은 하기 표 1에 나타난 바와 같으며, 상기 기초사료를 대조구로 하고, 상기 대조구에 기초사료의 전체중량 대비 우모분(Feather meal; FM) 3 중량% 첨가구(3% FM), 대조구에 우모분 3 중량% 및 피리독신(pyridoxin) 10ppm 첨가구(3% FM-피리독신), 대조구에 우모분 6 중량% 첨가구(6% FM) 및 대조구에 우모분 6 중량% 및 피리독신 10ppm 첨가구(6% FM-피리독신)를 각각 제조한 후 시험사료로 이용하였다. The blending ratio and components of the basic feed used in this test are as shown in Table 1 below, with the basic feed as a control, and added 3 wt% Feather meal (FM) to the total weight of the basic feed to the control. Household (3% FM), 3% by weight of feather powder and 10 ppm pyridoxin (3% FM-pyridoxine) in control, 6% by weight of feather (6% FM) and 6 in control Weight% and 10 ppm pyridoxine addition sphere (6% FM-pyridoxine) were prepared and used as test feeds.

성분(중량%)Ingredient (% by weight) 육성기Breeder 육성-비육기Upbringing 비육기Fattening 옥수수(Corn)Corn 58.1058.10 66.8866.88 71.7971.79 대두박(Soybean meal)Soybean meal 30.4730.47 22.4422.44 17.2717.27 석회석(Ca carbonate)Limestone (Ca carbonate) 0.220.22 0.310.31 0.350.35 인산칼슘(DEFL phosphorus)Calcium Phosphate (DEFL phosphorus) 1.461.46 1.411.41 1.061.06 식염(Salt)Salt 0.150.15 0.150.15 0.150.15 동물성 지방(Animal fat)Animal fat 4.474.47 4.234.23 3.993.99 당밀(malasses)Molasses 4.504.50 4.004.00 5.005.00 염화 콜린(Choline chloride)(60%)Choline chloride (60%) 0.070.07 0.030.03 0.050.05 CuSO4(34% Cu)CuSO 4 (34% Cu) 0.040.04 0.040.04 0.030.03 L-라이신(L-lysine)(78%)L-lysine (78%) 0.180.18 0.210.21 0.170.17 DL-메티오닌(DL-methionine)(98%)DL-methionine (98%) 0.050.05 0.040.04 0.010.01 항생제(antibiotic)Antibiotic 0.110.11 0.060.06 -- 비타민과 미네랄 혼합물1 Vitamin and Mineral Mixtures 1 0.200.20 0.200.20 0.130.13 합계Sum 100.00100.00 100.00100.00 100.00100.00 화학성분의 환산치Conversion of chemical components 대사에너지(Metabolizable Enery, ㎉/㎏)Metabolic energy (㎉ / ㎏) 3,300.003,300.00 3,300.003,300.00 3,300.003,300.00 조단백질(%)Crude Protein (%) 19.0019.00 16.0016.00 14.0014.00 라이신(%)Lysine (%) 1.131.13 0.960.96 0.790.79 칼슘(%)calcium(%) 0.700.70 0.700.70 0.600.60 인(%)sign(%) 0.610.61 0.570.57 0.480.48 1사료 ㎏ 당 공급량: vitamin A, 10,000 IU; vitamin D3, 2,000 IU; vitamin E, 42 IU; vitamin K, 5㎎; vitamin B2, 9.6㎎; vitamin B6, 2.45㎎; vitamin B12, 40㎍; pantothenic acid, 27㎎; niacin, 49㎎; biotin, 0.05㎎; Cu, 140㎎; Fe, 179㎎; Zn, 179㎎; Mn, 12.5㎎; I, 0.5㎎; Co, 0.25㎎; Se, 0.4㎎ First feed supply per ㎏: vitamin A, 10,000 IU; vitamin D 3 , 2,000 IU; vitamin E, 42 IU; vitamin K, 5 mg; vitamin B 2 , 9.6 mg; vitamin B 6 , 2.45 mg; vitamin B 12 , 40 μg; pantothenic acid, 27 mg; niacin, 49 mg; biotin, 0.05 mg; Cu, 140 mg; Fe, 179 mg; Zn, 179 mg; Mn, 12.5 mg; I, 0.5 mg; Co, 0.25 mg; Se, 0.4 mg

시험예 1. 시험동물의 준비 및 설계Test Example 1. Preparation and Design of Test Animals

사양시험을 위하여 3원 교잡종(Yorkshire x Landrace x Duroc)(경인축산) 육성 비육돈 135두를 공시하였으며, 시험개시시의 평균 체중은 46kg 이었다. 상기 실시예 1에서 제조한 5가지의 시험처리구를 3반복 반복당 9두(암컷: 4두, 거세수컷: 5두)로 하여 평사 스크레파식 돈방(4m x 3m)에 각 처리구별로 체중이 비슷한 개체들을 수용하여 완전 임의배치 하였다. For the specification test, 135 pigs were raised to raise three-way hybrids (Yorkshire x Landrace x Duroc) (Gyeongin livestock), and the average weight of the test was 46 kg. The five test treatments prepared in Example 1 were 9 heads per three repetitions (female: 4, castration male: 5), and the weight of each treatment in a plain screpa-type piglet (4m x 3m). Similar individuals were accepted and completely randomized.

실험기간 동안 물과 사료는 자유 급여되도록 하였으며, 체중 및 사료섭취량은 사양시험 개시 시와 개시일로부터 3주째인 육성기(grower stage), 8주째인 육성-비육기(grow-finisher stage) 및 11주째인 비육기(finisher stage)에 측정하여 기간별 일당 증체량, 일당 사료 섭취량 및 사료 요구율을 계산하였다.During the experimental period, water and feed were freely fed, and body weight and feed intake were at the growth stage at the start of the specification test and at the 3rd week from the start date, at the growth-finisher stage at the 8th week, and at the 11th week. Measurements were made at the finisher stage to calculate daily gain, daily feed intake and feed rate.

항목 Item 주 (week)Week 시험구Test SEM SEM 대조구Control 3% FM3% FM 3% FM + 피리독신3% FM + pyridoxine 6% FM6% FM 6% FM + 피리독신6% FM + pyridoxine 일당증체량 (g)Daily gain (g) 0~30-3 683.81683.81 815.71815.71 710.00710.00 770.00770.00 805.24805.24 50.7650.76 3~83 ~ 8 728.86728.86 714.57714.57 744.29744.29 725.14725.14 708.86708.86 63.4663.46 8~118 ~ 11 953.81953.81 947.62947.62 1013.811013.81 935.71935.71 924.29924.29 37.4737.47 0~110-11 777.92777.92 805.71805.71 808.44808.44 794.81794.81 793.90793.90 34.9934.99 사료섭취량 (g)Feed Intake (g) 0~30-3 1698.57b 1698.57 b 1962.86a 1962.86 a 1754.29ab 1754.29 ab 1889.05ab 1889.05 ab 1889.05ab 1889.05 ab 68.8068.80 3~83 ~ 8 2048.292048.29 2245.142245.14 2207.432207.43 2174.002174.00 2257.432257.43 151.8151.8 8~118 ~ 11 2877.142877.14 2997.142997.14 3058.573058.57 3029.053029.05 2898.572898.57 92.4792.47 0~110-11 2178.962178.96 2373.252373.25 2315.972315.97 2329.482329.48 2331.822331.82 92.1692.16 사료요구율 (g/g)Feed requirement (g / g) 0~30-3 2.482.48 2.412.41 2.472.47 2.452.45 2.352.35 0.110.11 3~83 ~ 8 2.812.81 3.143.14 2.972.97 3.003.00 3.183.18 0.160.16 8~118 ~ 11 3.02b 3.02 b 3.16ab 3.16 ab 3.02b 3.02 b 3.24a 3.24 a 3.14ab 3.14 ab 0.060.06 0~110-11 2.80b 2.80 b 2.95a 2.95 a 2.86ab 2.86 ab 2.93a 2.93 a 2.94a 2.94 a 0.030.03 폐사율(%)% Mortality 0~110-11 00 00 00 00 00 00 a-b 서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. ab Treatments with different superscripts differ significantly at P <0.05.

상기 표 2에 나타난 바와 같이, 육성기(0~3주)에서의 일당증체량은 우모분 첨가구들이 대조구에 비하여 높았으나 유의차는 나타나지 않았다. 사료섭취량은 3% FM구가 대조구에 비하여 유의하게 높았다(p<0.05). 사양시험 전 기간에서 일당증체량과 사료섭취량은 우모분 첨가구가 높게 나타났으나 유의차는 없었다. 한편, 사료효율은 우모분 첨가구들이 대조구보다 유의하게 떨어지는 결과를 나타내었다(p<0.05). 본 시험에서 우모분 첨가구의 증체율이나 사료섭취량이 낮은 것은 우모분의 이용율이 낮고 아미노산 조성이 양호하지 못하며, 우모분 3중량% 및 6 중량%의 단순첨가로 에너지/단백질 비율 균형이 맞지 않았기 때문인 것으로 보인다.As shown in Table 2, the daily weight gain in the growing period (0-3 weeks) was higher than the control group, but no significant difference appeared. Feed intake was significantly higher in the 3% FM group than the control group ( p <0.05). The daily weight gain and feed intake were higher in the fed group, but there was no significant difference. On the other hand, feed efficiency was significantly lower than the control group ( p <0.05). The low weight gain or feed intake of the feather powder added in this test was due to the low utilization of milk powder, poor amino acid composition, and the unbalanced energy / protein ratio due to the simple addition of 3% by weight and 6% by weight of milk powder. see.

시험예 2. 우모분 첨가에 따른 등지방 두께와 도체등급Test Example 2 Backfat Thickness and Carcass Grade According to Feather Addition

우모분 첨가에 따른 돈육의 등지방 두께와 도체등급을 측정하기 위해 샘플의 채취는 상기 시험예 1의 사양시험 종료 후, 각 처리 별로 암·수(거세돈) 각 6두씩, 총 60두를 도체 하여 등심, 안심, 햄, 심장 및 간을 채취한 후 분석 시까지 -50℃에 냉동 보관 하였다. 한편, 등지방 두께 측정은 각 처리별 12두씩 총 60두를 도축한 후 11 ~ 12번째와 마지막 늑골 부분을 측정하여 평균값으로 하였다. In order to measure the backfat thickness and the carcass grade of the pork with the addition of feather powder, the samples were collected. Sirloin, tenderloin, ham, heart and liver were collected and stored frozen at -50 ° C until analysis. On the other hand, the back fat thickness was measured as the average value by measuring the 11 to 12th and the last rib portion after slaughtering a total of 60 heads of 12 heads for each treatment.

항목Item 시험구Test SEM SEM 대조구Control 3% FM3% FM 3% FM + 피리독신3% FM + pyridoxine 6% FM6% FM 6% FM + 피리독신6% FM + pyridoxine 등지방 두께(㎜)Back fat thickness (mm) 20.83b 20.83 b 22.83ab 22.83 ab 22.08ab 22.08 ab 24.33ab 24.33 ab 25.42a 25.42 a 1.271.27 도체등급1 Conductor Class 1 1.751.75 1.751.75 2.082.08 1.501.50 2.252.25 0.300.30 1 1=등급A, 2=등급B, 3=등급C, 4=등급D a-b 서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. 1 1 = grade A, 2 = grade B, 3 = grade C, 4 = grade D ab Treatments with different superscripts differ significantly at P <0.05.

상기 표 3에 나타난 바와 같이, 등지방 두께는 우모분 첨가구들이 대조구에 비하여 유의적으로(p<0.05) 증가하였으나, 도체등급에 있어서 유의한 차이가 없었다.As shown in Table 3, the backfat thickness increased significantly ( p <0.05) in the feather added groups, but there was no significant difference in the carcass grade.

시험예 3. 우모분 첨가에 따른 돈육 내 타우린 함량의 분석Test Example 3 Analysis of Taurine Contents in Pork with Addition of Feathers

돈육 내 타우린 분석은 파올라 주닌의 분석방법(Paola Zunin, Filippo Evangelisti., Determination of free amino acids in infant formulars, International Dairy Journal, 9: pp653-656, 1999)에 따라 실시하였다. 얻어진 결과의 단위는 ㎍/g, fresh matter로 표시하였다.The analysis of taurine in pork was carried out according to the analysis method of paola junin (Paola Zunin, Filippo Evangelisti., Determination of free amino acids in infant formulars, International Dairy Journal , 9: pp653-656, 1999). The unit of the obtained result was represented by (micro | micron | gram) / g and fresh matter.

3-1. 샘플의 전처리3-1. Sample pretreatment

상기 시험예 2에서 냉동 보관된 돈육 샘플(sample), 즉 등심, 안심, 햄, 심장 및 간과 0.4M 과염소산(perchloric acid)을 1:5의 비율로 넣어 균질하고 난 후, 4℃에서 13,000rpm으로 20분 동안 원심분리하여 상층액을 수집하고 같은 과정을 2회 반복하였다. 수집된 상층액 2㎖를 음이온교환칼럼(anion-exchange-column, AG 50W-X8, 200~400mesh, H+form, 5×15㎜, Bio-Rad Laboratories)에 통과시킨 후, 다시 1㎖의 증류수로 3회 세척(washing)하여 총 5㎖의 추출액을 제조하였다.Pork samples frozen in Test Example 2, that is, sirloin, tenderloin, ham, heart and liver and 0.4M perchloric acid (homogeneous) in a ratio of 1: 5, and then homogeneous, at 13,000 rpm at 4 ° C. The supernatant was collected by centrifugation for 20 minutes and the same procedure repeated twice. 2 ml of the collected supernatant was passed through anion-exchange-column (AG 50W-X8, 200-400 mesh, H + form, 5 × 15 mm, Bio-Rad Laboratories), and then again 1 ml of distilled water. Washing three times with 3 to prepare a total of 5ml extract.

3-2. 고성능 액체 크로마토그래피(High-performance liquid chromatography; HPLC) 시스템3-2. High-performance liquid chromatography (HPLC) system

본 시험에 사용된 HPLC는 HPLC 모델 305 시스템(Gilson, France), 형광검출기 모델 121(Fluorometer Model 121, Gilson, Frnace) 및 머크 프리-칼럼(Merck LichroCART pre-column, Lichrospher 100 RP-18, 4×4㎜ ID)이 장착된 머크 칼럼(Merck LichroCART column, Superspher 100 RP-18 end-capped, 125× 4㎜ ID)을 이용하였다. 분석시 이동상으로는 0.8㎖/min 속도로 0.1M 소듐 아세테이트(sodium acetate)(pH 5.80)와 메탄올을 각각 80:20 (v/v)으로 혼합한 용액 A와 20:80 (v/v)으로 혼합한 용액 B를 흐르게 하여 형광분석기(fluorescence detector)(excitation wavelength: 350nm, emission wavelength: 426nm)로 측정하였으며, 이때 타우린은 12.56±0.05분 후에 분리되었다. 샘플 20㎕를 주입한 피크(peak)의 면적을 표준 타우린(standard taurine)(Sigma)과 각 시료 중 타우린의 얻어진 피크의 면적과 비교하여 계산하였다.The HPLC used for this test was HPLC Model 305 system (Gilson, France), Fluorometer Model 121 (Fluorometer Model 121, Gilson, Frnace) and Merck LichroCART pre-column, Lichrospher 100 RP-18, 4 × Merck LichroCART column (Superspher 100 RP-18 end-capped, 125 × 4 mm ID) equipped with 4 mm ID) was used. In mobile phase, 0.1M sodium acetate (pH 5.80) and methanol were mixed at 80:20 (v / v) and 20:80 (v / v) at 0.8 ml / min. One solution B was flowed and measured by a fluorescence detector (excitation wavelength: 350 nm, emission wavelength: 426 nm), at which time the taurine was separated after 12.56 ± 0.05 minutes. The area of the peak injected with 20 µl of the sample was calculated by comparing the area of the obtained peak of taurine in each sample with standard taurine (Sigma).

통계처리는 SAS (1995)의 프로그램 패키지(program package)의 GLM(General Linear Model) 방법에 의해 분산분석을 실시하였고, 처리 평균 간의 차이는 던컨스 다중 검정(Duncan's multiple range test)에 의해 유의성을 분석하였다.Statistical analysis was performed by variance analysis using the General Linear Model (GLM) method of the program package of SAS (1995), and the difference between the processing averages was analyzed by Duncan's multiple range test. It was.

3-3.O-프탈알데히드(O-phthalaldehyde; OPA) 유도반응3-3.O-phthalaldehyde (OPA) induction reaction

상기 시험예 3-2에서 생산된 추출액 중 100㎕를 OPA 유도체와 5분 동안 반응시킨 후, 칼럼을 통과시켜 형광검출기로 측정하여 1차 아미노산의 함량을 측정하였다. 이때, OPA 유도체는 OPA(25㎎)를 메탄올(methanol) 0.6㎖에 용해시킨 OPA 용액과 붕산나트륨(sodium tetraborate) 404㎎을 수조(water bath)에서 HPLC water(5㎖)에 녹인 후 식혀서 제조한 붕산용액(borate solution)을 각각 제조한 후, 상기 붕산용액을 OPA 용액에 첨가하고 형광물질을 제조하기 위하여 2-머캅토에탄올(2-mercaptoethanol) 25㎕를 더하여 혼합하여 제조하였다. 상기 방법으로 제조된 OPA 유도체는 4℃의 어두운 곳에 보관하고 12시간 이내에 사용하였다.After 100 μl of the extract produced in Test Example 3-2 was reacted with the OPA derivative for 5 minutes, the content of the primary amino acid was measured by measuring a fluorescent detector through a column. At this time, the OPA derivative was prepared by dissolving OPA (25 mg) in 0.6 ml of methanol and 404 mg of sodium tetraborate in HPLC water (5 ml) in a water bath and cooling. After each boric acid solution was prepared, the boric acid solution was added to the OPA solution, and 25 μl of 2-mercaptoethanol was added and mixed to prepare a fluorescent material. The OPA derivative prepared by the above method was stored in the dark at 4 ° C. and used within 12 hours.

항목Item 시험구Test SEM SEM 대조구Control 3% FM3% FM 3% FM + 피리독신3% FM + pyridoxine 6% FM6% FM 6% FM + 피리독신6% FM + pyridoxine 심장Heart 1393.80c 1393.80 c 1705.90bc 1705.90 bc 2056.10b 2056.10 b 2596.40a 2596.40 a 2667.10a 2667.10 a 129.60129.60 liver 647.30647.30 781.50781.50 762.00762.00 833.80833.80 859.80859.80 74.9174.91 안심relief 601.20c 601.20 c 654.80b 654.80 b 659.90b 659.90 b 728.80a 728.80 a 742.20a 742.20 a 18.6918.69 ham 462.40b 462.40 b 570.00ab 570.00 ab 580.40ab 580.40 ab 561.40ab 561.40 ab 588.40a 588.40 a 38.5538.55 등심sirloin 375.80b 375.80 b 417.90ab 417.90 ab 428.10ab 428.10 ab 463.50ab 463.50 ab 483.40a 483.40 a 31.1531.15 a-c서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. ac treatments with different superscripts differ significantly at P <0.05.

상기 표 4에 나타난 바와 같이, 대조구 사료를 급여하여 육성한 돈육에서 타우린 함량은 심장이 1393.80ppm 으로 가장 높았고 간이 647.30ppm, 안심이 601.20ppm, 햄이 462.40ppm, 등심이 375.80ppm순으로 나타났다. 심장, 안심, 햄 및 등심에서는 처리간에 유의차(p<0.05)가 있었으나 간에서는 유의차가 나타나지 않았다. 한편, 6% FM-피리독신 처리구의 경우, 대조구에 비해 심장, 안심, 햄 및 등심에서의 타우린 함량이 각각 91%, 23%, 27% 및 29% 증가하였다. 특히 우모분 첨가에 따른 심장의 타우린 함량이 증가하였다. 즉, 육성 비육단계(grow-finisher stage)나 비육단계(finisher stage)에서 사료 내 추가적 우모분 첨가가 생산성에는 영향을 미치지 않으나, 돈육 내 타우린 함량을 유의하게 증가시킴을 확인할 수 있었다.As shown in Table 4, the taurine content of the pigs fed the control diet was 1393.80ppm in the heart, liver 647.30ppm, tenderloin 601.20ppm, ham 462.40ppm, sirloin 375.80ppm in order. There was a significant difference ( p <0.05) between treatments in heart, tenderloin, ham and sirloin, but no difference in liver. On the other hand, 6% FM-pyridoxine treated group, the taurine content in the heart, tenderloin, ham and sirloin increased 91%, 23%, 27% and 29%, respectively, compared to the control. In particular, the taurine content of the heart increased with the addition of feather powder. In other words, the addition of supplementary milk powder in the feed at the growth-finisher stage or the finisher stage did not affect the productivity, but it was confirmed that the taurine content in the pork was significantly increased.

시험예 4. 타우린 함량이 증가된 돈육에 대한 관능검사Test Example 4 Sensory Evaluation of Pork with Increased Taurine Content

관능검사에 사용된 돈육은 사양시험 종료 후 각 처리구별로 평균 체중에 가까운 암·수(거세돈) 각 6두씩, 총 60두를 도축하여 등심과 삼겹살을 채취한 후 관능검사시까지 냉동 보관 하였다. 검사 재료는 신선육(raw meat)과 오븐에 넣고 200℃에서 20분간 조리한 조리육(boiled meat)으로 준비하였으며, 이를 다시 부위별로 구분하여 관능검사를 실시하였다. 평가항목은 신선육의 경우 향미(aroma), 육색(color), 불쾌치(off-flavor), 육즙의 삼출정도(purge), 전반적인 기호도(overall acceptability)를 조사하였으며, 조리육의 경우 향미(aroma), 풍미(flavor), 다즙성(juiciness), 육질의 부드러운 정도(tenderness), 전반적인 기호도(overall acceptability)를 조사하였다. Pork used for the sensory test was slaughtered for 60 heads of 6 males and females (neutered pigs) close to the average weight of each treatment group after the end of the specification test, and the loin and pork belly were collected and stored frozen until the sensory test. . The test material was prepared into raw meat and cooked meat cooked in an oven for 20 minutes at 200 ° C., and the sensory test was performed by dividing the meat into parts. The evaluation items were aroma, color, off-flavor, juice purge, and overall acceptability for fresh meat, and aroma for cooked meat. , Flavor, juiciness, tenderness of meat, and overall acceptability were investigated.

관능검사는 재료별로 각각 1회씩 실시하였으며 관능검사 패널은 훈련 받지 않은 일반 소비자 20명을 대상으로 하였다. 본 발명에 의해 생산된 돈육에 대한 소비자의 수용도를 파악하기 위하여 기호도 측정을 계량화할 수 있으면서도 기호도 결정에 중요하다고 생각되는 제품속성(Sensory Attributes)을 알아내기 위한 테스트(Hedonic Scale Acceptance Test)를 실시하고 사용할 척도의 구간은 5점을 사용하였는데 매우 좋은 것이 5점 그리고 매우 나쁜 것이 1점으로 배정하였다. 평가 속성으로 육즙의 삼출정도와 다즙성을 선정한 이유는 육질이 건조한 상태가 아니라 보수력(water holding capacity)을 가지고 있어야 품질이나 기호적인 측면에서 바람직하기 때문이며 점수가 높을수록 육질의 보수력이 높다고 평가하였다. 조리육의 평가 항목 중 다즙성은 재료를 8~9회 정도 씹었을 때 나오는 수분의 양을 가지고 이를 평가하도록 하였다. 또한 돈육을 조리한(broiling) 경우 고기에서 생성되는 독특한 향과 맛을 평가하기 위하여 향미와 풍미를 분리하여 평가하였으며 향미와는 달리 풍미는 고기를 씹었을 때 입안에서 느껴지는 전체적인 질감과 맛을 평가하는 것이다.The sensory test was conducted once for each material, and the sensory test panel was conducted for 20 untrained consumers. In order to grasp the consumer's acceptance of the pork produced by the present invention, it is possible to quantify the taste measurement, while conducting a test (Hedonic Scale Acceptance Test) to find out the sensory attributes that are considered important for determining the taste. 5 points were used as the intervals for the scales, and 5 points for the very good and 1 point for the very bad. The reason for selecting the degree of juice exudation and juiciness as an evaluation attribute is that it is desirable in terms of quality or preference in terms of water holding capacity, not dryness, and the higher the score, the higher the water retention capacity. Among the evaluation items of cooked meat, juiciness was made by evaluating the amount of moisture produced by chewing the material about 8-9 times. In addition, in the case of broiling pork, the flavor and flavor are separated and evaluated to evaluate the unique aroma and taste produced from the meat. Unlike the flavor, the flavor evaluates the overall texture and taste in the mouth when the meat is chewed. will be.

상기 시험예 4에 대한 결과를 하기 표 5, 6, 7, 8에 나타내었다.The results for Experiment 4 are shown in Tables 5, 6, 7, and 8 below.

신선육의 등심과 삼겹살에 대한 관능검사(수컷)Sensory Evaluation of Sirloin and Pork Belly in Fresh Meat (Male) 등심sirloin 삼겹살pork belly F-값F-value T1T1 T2T2 T3T3 T4T4 T5T5 평균Average T1T1 T2T2 T3T3 T4T4 T5T5 평균Average 향미Flavor 3.153.15 2.382.38 2.382.38 2.612.61 2.382.38 2.582.58 2.842.84 2.762.76 2.152.15 2.842.84 2.152.15 2.552.55 1.661.66 육색Meat color 2.53abc 2.53 abc 2.38abc 2.38 abc 2.00c 2.00 c 2.53abc 2.53 abc 2.15bc 2.15 bc 2.322.32 3.15a 3.15 a 2.76abc 2.76 abc 2.92ab 2.92 ab 3.00a 3.00 a 2.46abc 2.46 abc 2.862.86 2.192.19 불쾌취Displeasure 2.612.61 2.302.30 2.072.07 2.302.30 2.382.38 2.332.33 2.382.38 2.612.61 2.072.07 2.612.61 2.232.23 2.382.38 0.680.68 육즙의 삼출정도Juicy exudation 2.38bcd 2.38 bcd 1.92d 1.92 d 2.23bcd 2.23 bcd 2.15cd 2.15 cd 2.38bcd 2.38 bcd 2.212.21 3.23a 3.23 a 2.84abc 2.84 abc 2.92abc 2.92 abc 3.00ab 3.00 ab 2.53abcd 2.53 abcd 2.902.90 2.912.91 전반적인 기호도Overall preference 2.612.61 2.382.38 2.232.23 2.232.23 2.612.61 2.412.41 3.003.00 2.762.76 2.692.69 2.762.76 2.302.30 2.702.70 1.171.17 1T1; 대조구, T2; 3% FM, T3; 3% FM+피리독신, T4; 6% FM, T5; 6% FM+피리독신 첨가사료 a-d 서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. 1 T1; Control, T2; 3% FM, T3; 3% FM + pyridoxine, T4; 6% FM, T5; 6% FM + pyridoxine feed ad treatments with different superscripts were significantly different at P <0.05.

신선육의 등심과 삼겹살에 대한 관능검사(암컷)Sensory Evaluation of Sirloin and Pork Belly in Fresh Meat (Female) 등심sirloin 삼겹살pork belly F-값F-value T1T1 T2T2 T3T3 T4T4 T5T5 평균Average T1T1 T2T2 T3T3 T4T4 T5T5 평균Average 향미Flavor 2.842.84 2.762.76 2.922.92 2.532.53 2.002.00 2.612.61 2.382.38 2.462.46 2.302.30 2.762.76 2.382.38 2.462.46 1.691.69 육색Meat color 2.76ab 2.76 ab 2.23bc 2.23 bc 2.30bc 2.30 bc 2.15bc 2.15 bc 1.61c 1.61 c 2.212.21 3.46a 3.46 a 2.53b 2.53 b 2.46bc 2.46 bc 3.00ab 3.00 ab 2.53a 2.53 a 2.802.80 3.193.19 불쾌취Displeasure 2.612.61 2.762.76 2.612.61 2.382.38 2.072.07 2.492.49 2.382.38 2.072.07 2.072.07 2.612.61 2.382.38 2.302.30 1.281.28 육즙의 삼출정도Juicy exudation 2.382.38 2.612.61 2.462.46 2.612.61 1.841.84 2.382.38 2.762.76 2.302.30 2.382.38 2.842.84 2.532.53 2.562.56 1.011.01 전반적인 기호도Overall preference 2.76ab 2.76 ab 2.69ab 2.69 ab 2.69ab 2.69 ab 2.46abc 2.46 abc 1.84c 1.84 c 2.492.49 2.61ab 2.61 ab 2.46abc 2.46 abc 2.23bc 2.23 bc 3.07a 3.07 a 2.46abc 2.46 abc 2.572.57 2.052.05 1T1; 대조구, T2; 3% FM, T3; 3% FM+피리독신, T4; 6% FM, T5; 6% FM+피리독신 첨가사료 a-c서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. 1 T1; Control, T2; 3% FM, T3; 3% FM + pyridoxine, T4; 6% FM, T5; 6% FM + Pyridoxine feed ac Treated treatments with different superscripts were significantly different at P <0.05.

조리육의 등심과 삼겹살에 대한 관능검사(수컷)Sensory Evaluation of Sirloin and Pork Belly in Cooked Meat (Male) 등심sirloin 삼겹살pork belly F-값F-value T1T1 T2T2 T3T3 T4T4 T5T5 평균Average T1T1 T2T2 T3T3 T4T4 T5T5 평균Average 향미Flavor 3.263.26 3.333.33 3.003.00 3.263.26 2.802.80 3.133.13 2.782.78 2.712.71 3.073.07 3.283.28 3.213.21 3.013.01 1.051.05 풍미zest 3.53a 3.53 a 3.13ab 3.13 ab 3.00abc 3.00 abc 2.86abc 2.86 abc 2.33c 2.33 c 2.972.97 2.57bc 2.57 bc 2.57bc 2.57 bc 2.71bc 2.71 bc 3.14ab 3.14 ab 3.14ab 3.14 ab 2.832.83 2.612.61 다즙성Succulent 2.862.86 2.862.86 2.602.60 2.732.73 3.063.06 2.822.82 3.143.14 3.003.00 3.003.00 3.213.21 3.783.78 3.233.23 1.831.83 육질의 부드러운정도The softness of meat 3.00b 3.00 b 2.60bc 2.60 bc 2.66bc 2.66 bc 2.20bc 2.20 bc 2.13c 2.13 c 2.522.52 2.57bc 2.57 bc 2.71bc 2.71 bc 2.78bc 2.78 bc 3.78a 3.78 a 4.21a 4.21 a 3.213.21 6.496.49 전반적인 기호도Overall preference 3.40abc 3.40 abc 3.00bcd 3.00 bcd 2.73d 2.73 d 2.73d 2.73 d 2.46d 2.46 d 2.862.86 2.85cd 2.85 cd 2.85cd 2.85 cd 3.07bcd 3.07 bcd 3.57ab 3.57 ab 3.92a 3.92 a 3.253.25 4.584.58 1T1; 대조구, T2; 3% FM, T3; 3% FM+피리독신, T4; 6% FM, T5; 6% FM+피리독신 첨가사료 a-d 서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. 1 T1; Control, T2; 3% FM, T3; 3% FM + pyridoxine, T4; 6% FM, T5; 6% FM + pyridoxine feed ad treatments with different superscripts were significantly different at P <0.05.

조리육의 등심과 삼겹살에 대한 관능검사(암컷)Sensory Evaluation of Sirloin and Pork Belly in Cooked Meat (Female) 등심sirloin 삼겹살pork belly F-값F-value T1T1 T2T2 T3T3 T4T4 T5T5 평균Average T1T1 T2T2 T3T3 T4T4 T5T5 평균Average 향미Flavor 2.662.66 3.133.13 3.333.33 2.862.86 3.063.06 3.013.01 3.423.42 2.642.64 2.712.71 2.712.71 2.782.78 2.852.85 1.741.74 풍미zest 2.662.66 2.932.93 3.133.13 3.063.06 2.862.86 2.932.93 3.213.21 2.642.64 2.572.57 2.502.50 3.003.00 2.782.78 1.801.80 다즙성Succulent 2.932.93 2.732.73 3.003.00 2.662.66 3.003.00 2.862.86 3.283.28 2.572.57 2.572.57 2.782.78 3.073.07 2.852.85 1.081.08 육질의 부드러운 정도Tenderness of meat 2.60bcd 2.60 bcd 2.53bcd 2.53 bcd 2.86abc 2.86 abc 2.53bcd 2.53 bcd 2.40cd 2.40 cd 2.582.58 3.50a 3.50 a 2.42cd 2.42 cd 2.00d 2.00 d 3.21ab 3.21 ab 3.00abc 3.00 abc 2.832.83 3.183.18 전반적인 기호도Overall preference 2.73b 2.73 b 3.06ab 3.06 ab 3.06ab 3.06 ab 2.73b 2.73 b 2.86ab 2.86 ab 2.892.89 3.35a 3.35 a 2.50b 2.50 b 2.50b 2.50 b 2.92ab 2.92 ab 3.00ab 3.00 ab 2.852.85 2.062.06 1T1; 대조구, T2; 3% FM, T3; 3% FM+피리독신, T4; 6% FM, T5; 6% FM+피리독신 첨가사료 a-d서로 다른 윗첨자를 가진 처리구는 P<0.05 수준에서 유의적으로 다르다. 1 T1; Control, T2; 3% FM, T3; 3% FM + pyridoxine, T4; 6% FM, T5; 6% FM + pyridoxine feed ad treatments with different superscripts were significantly different at P <0.05.

신선육의 경우, 상기 표 5에 나타난 바와 같이 수컷(거세돈)의 육색과 육즙 삼출 정도에서 삼겹살의 대조구가 각각 3.15점과 3.23점으로 가장 높은 점수를 나타내었다. 등심과 삼겹살의 속성별 평균치를 비교하였을 때 향미를 제외한 모든 속성에서 삼겹살이 등심보다 더 좋은 점수를 나타내었다. 또한, 상기 표 6에 나타난 바와 같이 암컷에서는 육색과 전반적인 기호도에만 유의차가 관찰 되었다. 육색의 경우 삼겹살의 대조구가 3.46점으로 가장 좋은 점수를 나타내었으며, 등심의 6% FM-피리독신 처리구가 1.61점으로 가장 낮은 점수를 나타내었다. 전반적인 기호도의 경우 삼겹살의 6% FM 처리구가 3.07점으로 가장 좋게 평가 되었으며, 등심의 6% FM-피리독신 처리구가 1.84점으로 가장 낮은 것으로 평가 되었다. 부위별 속성의 평균치를 비교하였을 때에는 육색, 육즙의 삼출정도 및 전반적인 기호도에서 삼겹살이 등심보다 더 좋은 점수를 나타내었다.In the case of fresh meat, the control group of pork belly showed the highest scores of 3.15 points and 3.23 points, respectively, in meat color and juicy effusion of males (kerate) as shown in Table 5 above. When comparing the mean values of sirloin and pork belly, the pork belly scored better than sirloin in all properties except flavor. In addition, as shown in Table 6, significant differences were observed only in meat color and overall acceptability in females. In case of meat color, the control group of pork belly showed the best score with 3.46 points, and the 6% FM-pyridoxine treated group showed the lowest score with 1.61 points. In terms of overall acceptability, the 6% FM treatment of pork belly was best rated at 3.07 points, and the 6% FM-pyridoxine treatment of sirloin was lowest at 1.84 points. When comparing the mean values of each region, pork belly had better scores than sirloin in meat color, juice exudation, and overall preference.

조리육의 경우, 상기 표 7에 나타난 바와 같이 수컷(거세돈)의 풍미는 등심의 대조구가 3.53점으로 가장 좋다고 평가 되었으며, 다즙성, 연도 및 전반적인 기호도에는 삼겹살의 6% FM-피리독신 처리구가 가장 좋게 평가 되었으며(p<0.05), 등심의 3% FM-피리독신 처리구와 6% FM-피리독신 처리구가 가장 낮은 점수를 나타냈다(p<0.01). 처리구별 평균치에서 보면 향미와 풍미를 제외하고는 삼겹살이 등심에 비해 더 좋은 점수를 나타내었다. 또한, 상기 표 8에 나타난 바와 같이 암컷에서는 육질의 부드러운 정도와 전반적인 기호도에서만 유의차가 관찰되었는데, 이 두 가지 속성에 대해 삼겹살의 대조구가 가장 높은 점수를 나타냈으며, 속성별 평균치를 비교할 경우 육질의 연도를 제외하고는 모든 속성에서 등심이 삼겹살 보다 더 좋은 점수를 나타냈다. 이상의 관능검사를 종합적으로 살펴보면 처리간에 일정한 경향치는 나타나지 않아 우모분 및 피리독신의 처리에 의해 돈육의 기호성에 큰 변화가 없는 것으로 사료된다.In the case of cooked meat, the taste of the male (caster pig) was evaluated to be the best as the control of the sirloin with a 3.53 point, and 6% FM-pyridoxine treatment of pork belly was the best in the juiciness, year and overall preference. was assessed (p <0.05), a 3% FM- pyridoxine treatment with 6% FM- pyridoxine treatment of fillets showed the lowest score (p <0.01). The average value of the treatment groups showed that pork belly had a better score than sirloin except for flavor and flavor. In addition, as shown in Table 8, significant differences were observed only in the softness and overall preference of the meat in females. The control score of pork belly showed the highest score for the two properties, and the year of meat quality was compared when comparing the average value of each property. Except for, the sirloin scored better than pork belly in all properties. Comprehensive examination of the sensory test did not show a constant tendency between treatments, so it seems that there is no significant change in the palatability of pork by the treatment of feather powder and pyridoxine.

본 발명은 상술한 특정의 실시예, 시험예, 도면 또는 도표에 기재된 내용에 기술적 사상이 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형의 실시가 가능한 것은 물론이고, 그와 같은 변경은 청구범위 기재의 범위 내에 있게 된다.The present invention is not limited to the technical spirit described in the specific embodiments, test examples, drawings or diagrams described above, and the general knowledge in the technical field to which the present invention belongs without departing from the gist of the present invention as claimed in the claims. Of course, any person having a variety of modifications can be made, and such changes are within the scope of the claims.

상기에서 상세히 설명한 바와 같이, 본 발명은 우모분 및 피리독신을 첨가한 사료를 급여하여 타우린 함량이 증가된 돈육의 생산방법 및 상기 방법에 의해 생산된 돈육에 관한 것으로, 본 발명에 의하면 가금육의 소비 후 폐기처리되는 우모를 사료로 재활용할 수 있고, 타우린 함량이 증가된 기능성 돈육을 생산하도록 함으로써 타우린 섭취가 부족한 사람들에게 타우린을 간편하게 공급해 줄 수 있다.As described in detail above, the present invention relates to a method of producing pork with increased taurine content by feeding feed with feather powder and pyridoxine, and to pork produced by the method according to the present invention. The discarded cows can be recycled as feed, and by producing functional pork with increased taurine content, the taurine can be easily supplied to people who are not consuming taurine.

Claims (4)

삭제delete 삭제delete 사료 전체 중량대비 1~10 중량%의 우모분과 5 ~ 20 ppm의 피리독신을 함유한 사료로 사양된 것을 특징으로 하는 타우린 함량이 증가된 돈육의 생산방법. A method of producing pork with increased taurine content, characterized in that it is characterized by a feed containing 1 to 10% by weight of the nutrient content and 5 to 20 ppm of pyridoxine relative to the total weight of the feed. 제 3항의 방법으로 생산된 타우린 함량이 증가된 돈육.Pork with increased taurine content produced by the method of claim 3.
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