KR100647082B1 - The production method of broiler meat containing taurine by using the feed containing feather meal and pyridox and thereof broiler meat - Google Patents

Abstract

The present invention relates to a method for producing taurine-enhanced chicken meat, characterized in that it is specified as feed containing cow's milk powder and chicken meat produced by the above method, and more specifically, by feeding the feed containing cow's milk powder and pyridoxine. It relates to a method for producing chicken with increased taurine and chicken produced by the method.

In the present invention, the content of taurine is increased in the leg muscles of broiler broiler fed untreated cow powder in the late broiler season, and the content of taurine in broiled chicken broiler containing feed powder and pyridoxine during whole broiler breeding period is significant. It is effective to increase.

Dairy powder, pyridoxine, taurine, poultry, feed

Description

TECHNICAL METHOD OF BROILER MEAT CONTAINING TAURINE BY USING THE FEED CONTAINING FEATHER MEAL AND PYRIDOX AND THEREOF BROILER MEAT}

Figure 1 shows the taurine content of the leg muscles of broiler chickens fed with feed containing the feather according to the present invention.

Figure 2 shows the taurine content of the pectoral muscles of broiler broiler diets containing feather powder according to the present invention.

Figure 3 shows the taurine content of the heart muscle of broiler broiler feed containing the feather according to the present invention.

Figure 4 shows the taurine content of leg muscles of broiler chickens fed with feed containing cow feather powder and pyridoxine according to the present invention.

Figure 5 shows the taurine content of the pectoral muscles of broilers broiled as feed containing feather powder and pyridoxine according to the present invention.

Figure 6 shows the taurine content of the liver tissue of broiler broiler diets containing feather powder and pyridoxine according to the present invention.

The present invention relates to a chicken production method in which taurine is increased to broiler, which is specified as feed containing feather powder and pyridoxine, and chicken produced by the method.

In connection with the present invention, the feather powder is dried and pulverized by heating the feathers of poultry under high pressure, and the content of cystine, a precursor of taurine, is 4.34% (NRC, 1994), which is the highest among livestock feed. Feather powder has high digestibility and good feed value compared to the dried one, but it is treated as a kind of pollution industry due to strong wastewater and odor during the pretreatment process, and the utilization of protein was very low (Moran et al., 1966; Morris and others). Balloun. 1973). Digestion rate of raw milk protein was 7.7%, but the quality of high quality milk powder protein was about 80% (McCasland and Richardson, 1966). Feathers contain more than 85% crude protein, which means that they can be supplemented with scarce animal proteins and wastes. However, 85 to 90% of feather protein is derived from keratin (Harrap and Woods, 1964). Its structure consists of peptide bonds that form beta-helix and numerous hydrogen bonds in the chain, which are twisted like cables by disulfide bonds, physically and chemically. It has a stable form and is difficult to be digested easily by digestive enzymes (Schor and Krimm; 1961; Moran et al., 1966). In particular, the poor balance of amino acids results in a lack of methionine, lysine, histidine, tryptophan, and feather protein, which has very low digestibility, which reduces feed value. It is becoming a factor.

On the other hand, taurine is involved in various essential physiological functions in the body, pyridoxine (pyridoxine) plays an important role as a coenzyme in synthesizing from cystine and enhance the vitality of the enzymes involved.

To date, there have been attempts to develop taurine-enriched livestock products, but there are no studies on the use of feather powder and pyridoxine treated with various methods.

An object of the present invention for solving the above problems is to provide a method for producing taurine-enhanced poultry by supplying a cow's powder containing cystine, which is a precursor of taurine, and pyridoxine to help enzyme activity in order to increase taurine content. To provide.

It is also an object of the present invention to provide a chicken with increased taurine produced by the above method.

The present invention for achieving the above technical problem relates to a poultry production method with increased taurine as a broiler characterized as feed containing cow's powder and pyridoxine, and a poultry produced by the method.

Hereinafter, the configuration of the present invention will be described in detail.

The present inventors are a source of cystine, a precursor of taurine, to add broth powder and synthetic taurine, respectively, which have been processed with acid or alkali to improve the utilization rate of the hair powder and the hair powder to broilers. Tests for broiler weight gain, feed intake, feed demand, mortality and chest muscle, leg muscle, and taurine content in the heart.

In addition, the inventors of the present invention are a source of cystine, a precursor of taurine, a feed containing a feather powder, a feed containing a feather powder and pyridoxine, a feed containing a decomposition product of a feather powder treated with hydrogen peroxide, and a powdered enzyme powder. By examining broilers with feed containing degradation products, the effects on broiler weight gain, feed intake, feed demand and mortality are tested.

In addition, the inventors of the present invention, breast meat collected from a broiler characterized by feed containing nutrient powder, feed containing nutrient powder and pyridoxine, feed containing nutrient dehydrogenated hydrogen peroxide, feed containing enzyme soluble nutrient powder, The increase in taurine in the leg muscles and liver tissues is measured.

In addition, the inventors of the present invention, the leg muscles and breasts of the meat specification, respectively, with feed containing feather powder, feed containing feather powder and pyridoxine, feed containing hydrogen peroxide-treated digest powder, feed containing enzyme-treated digest powder Samples of muscles are taken to evaluate flavor, flesh color, unpleasantness, effusion level and overall desirability in fresh meat, and flavor, flavor, juiciness, tenderness of meat and overall desirability in seasoned meat. do.

Hereinafter, the configuration of the present invention will be described in detail through examples, test examples, drawings and diagrams. However, the scope of the present invention is not limited to the claims of the present invention by examples, test examples, drawings and diagrams.

In Examples 1 and 2 and Test Examples 1 to 3 below, the broiler was designated as feed containing feather powder, acid or alkaline treated feather powder, and taurine, respectively, to measure the weight gain, feed intake, feed demand, and mortality of broilers. In addition, the leg muscles, chest muscles, heart muscles of broiler chickens specified as the respective feeds are collected to measure the taurine content.

Example 1

The blending ratio and components of the basic feed (control) used in this test are shown in Table 1 below. Using this basic feed as a control, 5 wt% Feather meal (FM), 4 wt% NaOH-treated feed (NaOH-FM), nitric acid (HNO) ₃ ) Treated feather powder 5% by weight of feed (HNO ₃ -FM), and 0.5% by weight of synthetic taurine (Taurine) was added to the test feed.

Ingredients and Contents of Basic Feed ingredient Content (% by weight) Corn 55.88 Soybean meal (44% CP) 31.52 Corn gluten 3.57 Animal fat 4.00 Calcium Phosphate (Calphos-18) 1.70 Rapeseed meal 1.30 Fish meal 1.00 Limestone 0.38 Salt 0.20 Vitamin premix 0.15 Mineral premix 0.10 Sum 100 Design sum Metabolizable Energy (㎉ / ㎏) 3,150 Crude Protein (%) 22.00 Calcium (%) 0.95 Lysine (%) 1.18 Methionine & Cystine (%) 0.90 Soluble Phosphorus (Phos-Avail,%) 0.25 ^First feed supply per ㎏: vitamin A, 13,500 IU; vitamin D ₃ , 3375 IU; vitamin E, 33.8 mg; vitamin K ₃ , 2.3 mg; vitamin B ₁ , 2.7 IU; vitamin B ₂ , 6.8 mg; vitamin B ₆ , 4.1 mg; vitamin B ₁₂ , 21.6 μg; biotin, 113.4 μg; niacin, 45.9 mg; pantothenic acid, 12.2 mg; folic acid, 1.4 mg. ² contents / kg: Zn, 67.5 g; Mn, 67.5 g; Fe, 67.5 g; Cu, 6.8 g; I, 1.5 mg; Se, 0.4 mg

Example 2 Preparation of Feather Digest

Fecal powder was hydrolyzed at 60 ° C. for 1 hour with respective 1N-sodium hydroxide (NaOH) solution, 1N-nitric acid (HNO ₃ ) solution, and neutralized with 1N-hydrochloric acid (HCl) solution and 1N-sodium hydroxide (NaOH) solution. Fecal powder decomposition product was prepared.

The feather powder treated with the 1N-nitric acid (HNO ₃ ) solution was removed by sodium nitrate (NaNO ₃ ), a salt produced by treating with nitric acid (HNO ₃ ) solution, and then dried and ground to prepare a wool powder decomposition product.

Test Example 1

For the specification test, 100 freshly hatched broilers (Ross®) were disclosed, and 5 treatments were repeated twice, and the steel cage (width: 90 cm, length: 90) was repeated twice for 10 repetitions (5 males and 5 males) per repetition. Cm, height: 50 cm). The test was conducted for 42 days, and control broilers were fed to all broilers in all treatments from 1 to 21 days of the 42 days, and test feeds from 22 to 42 days of age. During the test period, water and feed were free-vegetated, lights were turned on for 24 hours, and weight gain, feed intake, feed demand and mortality of broilers were measured weekly.

The results according to Test Example 1 are shown in Table 2 below.

Effects of Feather Additive Feed, Acid Treated Feather Additive Feed, Alkaline Treated Feather Additive Feed, and Taurine Additive Feed on the Weight Gain, Feed Intake, Feed Demand, and Mortality of Broilers  Item Week Test Zone¹  SEM Control Feather NaOH-feather powder HNO₃-feather powder Taurine Broiler weight gain (g / bird) 0-3 699.0 687.1 709.1 718.2 691.6 21.045 4 ~ 6 1465.8 1355.3 1325.8 1447.3 1356.8 54.703 0-6 2164.8 2042.3 2034.8 2165.4 2048.4 36.283 Feed Intake (g / bird) 0-3 973.9 943.0 942.8 1021.3 949.5 44.823 4 ~ 6 2700.4 ^a 2440.0 ^b 2623.5 ^ab 2550.0 ^ab 2492.5 ^ab 56.784 0-6 3674.3 ^a 3382.9 ^b 3566.3 ^ab 3571.3 ^ab 3442.0 ^b 70.013 Feed requirement (g / g) 0-3 1.39 1.37 1.33 1.42 1.37 0.023 4 ~ 6 1.84 ^ab 1.80 ^ab 1.98 ^a 1.76 ^b 1.84 ^ab 0.051 0-6 1.69 ^ab 1.66 ^b 1.75 ^a 1.65 ^b 1.67 ^ab 0.002 % Mortality 0-6 0 0 0 0 0 0 ^ab Treatments with different superscripts differ significantly at P <0.05. ¹ feather powder; Feed with 5% by weight of feather powder, NaOH-FM; Feed supplemented with 5% by weight NaOH treatment, HNO ₃ -FM; Feed supplemented with 5% by weight of feather powder treated with HNO ₃ , taurine; Synthetic Taurine 0.5% Additive Feed

As shown in Table 2, the test groups were higher than the control group in weight gain, but there was no significant difference.

Feed intake was lower in taurine and uranium (FM) feeds than in control, and thorium (NaOH) and nitrate (HNO ₃ ) -treated feathers showed higher trends than feathers.

In addition, the feed demand rate was higher in the sodium hydroxide (NaOH) -treated woofer group than in the nitric acid (HNO ₃ ) -treated woofer and woomin groups, but there was no significant difference between the control and taurine addition groups.

Low weight gain or feed intake of feather powder or feather digesters in this study was low in milk powder availability, poor amino acid composition, and 5% by weight of feather powder or feather digest as a simple addition of energy / protein ratio. Because you can't balance. It is expected that the addition of taurine or the cystine from the feathers will be converted to taurine in the body and affect growth, but many researchers have reported that taurine is in the process of growth. It is thought to have an early effect.

Example 3 Sample Collection

After completion of the specification test of the control and four test specimens (42 days old), the chest muscles, the leg muscles and the heart muscles were collected by carcassing a total of 100 heads, each of 5 males and 5 males, which were close to the average body weight, per treatment. Frozen at -50 ° C until analysis.

Test Example 2 Taurine Analysis

Taurine analysis was based on Paola Zunin et al. (1999). The unit of the obtained result was represented by (micro | micron | gram) / g and fresh matter.

전 Sample preparation

Homogenized frozen sample and 0.4M perchloric acid in a ratio of 1: 5, homogenized, and centrifuged at 13,000 rpm for 20 minutes at 4 ° C to collect supernatant. Repeated times.

2 ml of the supernatant was passed through anion-exchange-column (AG 50W-X8, 200-400 mesh, H + form, 5 × 15 mm, Bio-Rad Laboratories) and washed 1 ml of distilled water three times. A total of 5 ml extract was prepared by washing.

Performance High-performance liquid chromatography (HPLC) system

The HPLC used in this test was HPLC Model 305 system (Gilson, France), Fluorometer Model 121 (Gilson, Frnace), Merck LichroCART column (Superspher 100 RP-18 end-capped, 125 × 4 mm ID) combined with a Merck LichroCART pre-column (Lichrospher 100 RP-18, 4 × 4 mm ID)

⑶ O-phthalaldehyde (OPA) induction reaction

In order to measure the content of only the primary amino acid, 100 μl of the extract was reacted with the OPA derivative for 5 minutes, and then passed through a column to measure the fluorometer.

OPA (25 mg) was dissolved in 0.6 ml of methanol, and 404 mg of sodium tetraborate was dissolved in HPLC water (5 ml) in a water bath and cooled. A boric acid solution (borate solution) was added to the OPA solution (OPA solution ①) and 25 μl of 2-mercaptoethanol was added and mixed to prepare a fluorescent material. OPA derivatives were stored in the dark at 4 ° C. after preparation and used within 12 hours (Pittaluga et al., 1977).

Test Example 3

Statistical processing of the data obtained in Test Example 1 was analyzed using GLM (General Linear Model) procedure of SAS (1995), and the difference between the treatment averages was tested at P <0.05 by Duncan's multiple range test.

The results by Test Examples 2 and 3 are shown in FIGS. 1, 2 and 3.

As shown in FIGS. 1, 2 and 3, the taurine content of the control alone was the highest in the order of heart muscle in the order of 1482 ㎍ / g, leg muscle 778 ㎍ / g, and chest muscle in the order of 79 ㎍ / g. appear. For leg and chest muscles, there was a significant difference (P <0.01) between treatments, but no difference in heart.

As shown in FIG. 1, in the case of leg muscles, the taurine content of FM, FM digest, and synthetic taurine added groups was significantly higher than that of the control group, which was 170% higher in the taurine group than the control group. 123% added, 122% added NaOH-FM and 63% added HNO ₃ -FM.

As shown in FIG. 2, in the pectoral muscle, the synthetic taurine treatment was 246% higher than that of the control, but there were no significant differences in the other treatments (hair powder, feather digest). Heart muscle had the highest taurine content compared to other muscles, but there was no significant difference among all treatments. In broilers, there was a big difference in the taurine content according to the muscle area. The breast muscle, which is involuntary and white, was about 1/10 of the leg muscle, which is voluntary and red.

As shown in FIG. 3, taurine content in the heart muscle was the highest, but was not affected by the treatment.

According to the study, “Myocardial dysfunction (Schaffer et al., 1982) occurred in turkey when taurine content in heart muscle decreased, and the incidence rate of meat bone meal decreased in relation to the sudden death of broilers. ), But it was not found to be related to the sudden death of broilers and the taurine content in heart muscle (Jacob et al., 1991). ”This is consistent with the test results of the present invention. It was.

Comprehensive review of the above results in the addition of feather powder or taurine (taurine) in the late broiler, the productivity is not improved, but the taurine content in the leg muscles could be significantly increased.

Taurine content varies greatly between muscles, the highest content of heart muscle is not significantly affected by the treatment, and leg muscles contain about 10 times more taurine than breast muscle and 5% by weight feathering treatment Increased taurine content by 170%. On the other hand, the treatment of feather powder with sodium hydroxide (NaOH) and nitric acid (HNO ₃ ) did not help to strengthen taurine.

In Examples 3 to 5 and Test Examples 4 to 5, the broiler was added with each of the feather powder, the feather powder and pyridoxine, hydrogen peroxide or the enzyme-treated cow powder decomposition products, and the weight gain, feed intake, feed demand and mortality of the broilers were compared and measured. do. In addition, the leg muscles, breast muscles, liver tissue of the broilers specified as the feed for each of the taurine content is measured and compared.

Example 3

The blending ratio and components of the basic feed (control) used in this test are shown in Table 3 below. The starter feed was given for 3 weeks and the finisher feed for 2 weeks. This feed was used as a control, and 5% by weight of milk powder, 5% by weight of milk powder + 21 mg / kg of pyridoxin, 5% by weight of milk peroxide (H ₂ O ₂ ) treated enzyme, enzyme Five test strips were placed, including feed containing 5% by weight of the treated feather powder.

Ingredients (content, weight%) and nutritional ingredients of basic pigment Element Starter Finisher Corn 42.82 41.80 Soybean meal 20.02 18.24 Wheat 15.00 15.00 Wheat bran 5.00 5.00 Corn gluten 3.22 2.26 Tallow 2.81 4.93 Lupin 3.00 3.00 Calcium Phosphate (Dical-P) 1.62 1.35 Animal oil meal 1.00 1.50 Rapeseed meal 1.00 1.00 Rice bran 0.50 2.00 Fish meal 1.00 1.00 Limestone 0.99 0.96 Lysine 0.71 0.60 Additives¹ 0.56 0.59 Salt 0.20 0.17 Methionine 0.18 0.11 Vitamin mixture (Vitamin premix²) 0.15 0.15 Mineral premix³ 0.12 0.12 Electrolyte 0.12 Choline chloride 0.10 0.10 Sum 100 100 Design total Metabolic energy (Metabolizable, ㎉ / ㎏) 3050 3170 Crude Protein (%) 19.50 19.00 Calcium (%) 0.95 0.90 Phosphorus (%) 0.69 0.66 ¹ additive: electricity; clopidol, avilamycin, tetracycline, mycobond (toxin binder), oxyguard (oxbile), pigment, herbal extract, probiotics, later; maduramycin, avilamycin, tetracycline, mycobond (toxin binder), oxyguard (oxbile), herbal extract, probiotics. ² Feed per kg feed: vitamin A, 12,000 IU; vitamin D, 2500 IU; vitamin E, 40 mg; vitamin K, 2 mg; vitamin B ₁ , 2 mg; vitamin B _2, 5 mg; vitamin B ₆ , 3 mg; vitamin B ₁₂ , 20 mg; biotin, 150 mg; niacin, 40 mg; folic acid, 1.0 mg; Ca-PAN, 10 mg; Antioxidant, 60 mg. ³ content / kg: Zn, 90 mg; Mn, 96 mg; Fe, 50 mg; Cu, 24 mg; I, 1.2 mg; Se, 0.36 mg.

Example 4 Preparation of Feather Digest

Hydrogen peroxide (H ₂ O ₂ ) -treated feather powder was added to 20 kg of feather powder, 20 liters of water and 600 ml of hydrogen peroxide (H ₂ O ₂ ) were added and reacted for 12 hours, followed by 600 ml of 5N-sodium hydroxide (5N-NaOH). After the reaction was carried out for 12 hours, the enzymatic digestion was performed by adding 30 ml of Devolase (Vision Biochem Co., Ltd.) to 5 liters of water and reacting for 12 hours, followed by drying and grinding.

Test Example 4

1000 freshly hatched broiler chickens (Ross®) were published and 50 birds (25 females and 25 females) per 4 treatments were placed in a flat cage (width: 200 cm, length: 240 cm). During the 35-day feeding period, water and feed were free-vegetated, lights were lit for 24 hours, and weekly gains, feed intake, feed demand, and mortality of broilers were measured.

Statistical processing of the measured data was analyzed using the General Linear Model (GLM) procedure of SAS (1995), and the difference between treatment averages was tested at P <0.01 by Duncan's multiple range test.

The results according to Test Example 4 are shown in Table 4 below.

Effects of Feather Additives, Feathers and Pyridoxine Feeds, Hydrogen Peroxide-treated Feathered Additives, and Enzyme-treated Feathered Additives on Weight Gain, Feed Intake, Feed Demand, and Mortality in Broilers Item Weeks (wks) Test Zone¹ SEM Control Feather Feces + Pyridoxine Hydrogen Peroxide-Feathers Enzyme-Feather Broiler weight gain (g / bird) 0-3 718.4 ^ab 692.8 ^bc 723.3 ^a 664.4 ^c 680.6 ^c 9.32 4 ~ 5 956.5 890.7 904.8 901.8 916.6 31.16 0-5 1674.9 1583.5 1628.1 1566.3 1597.2 34.25 Feed Intake (g / bird) 0-3 1098.3 ^a 1056.0 ^ab 1081.8 ^a 996.5 ^b 1052.4 ^ab 20.47 4 ~ 5 1859.4 ^a 1813.9 ^ab 1722.6 ^bc 1823.5 ^ab 1626.4 ^c 35.96 0-5 2957.7 ^a 2869.8 ^ab 2804.4 ^bc 2820.0 ^abc 2675.8 ^c 45.51 Feed requirement (g / g) 0-3 1.53 1.53 1.50 1.50 1.55 0.020 4 ~ 5 1.95 ^a 2.04 ^a 1.91 ^ab 2.03 ^a 1.78 ^b 0.046 0-5 1.77 ^ab 1.81 ^b 1.72 ^bc 1.80 ^a 1.68 ^c 0.022 % Mortality 0-3 0.5 0 0 0 1.5 0.49 4 ~ 5 1.0 0 0.5 0 0 0.31 0-5 1.5 0 0.5 0 1.5 0.60 ^ac treatments with different superscripts differ significantly at P <0.05. ¹ FM; Feed with 5% by weight of feather powder, FM + Pyridox; 5% by weight of feather powder + Pyridoxine 21 mg / kg feed, H ₂ O ₂ -FM; Feed supplemented with 5% by weight H ₂ O ₂ -treated feather powder, Enzyme-FM; Addition of 5% by weight of enzyme-treated feather powder

As shown in Table 4, there was a significant difference in weight gain up to 3 weeks of age (P <0.05), but there was no statistically significant difference thereafter. The weight gain up to 3 weeks of age was the highest in the feather added and pyridoxine 21 mg / kg (FM + Pyridox) added diets.

In addition, there was a significant difference in feed intake for the whole period. The control was the highest and the enzyme-feather supplement was the lowest.

In addition, there were significant differences in feed demand rate between late stage and whole period. Feather and hydrogen peroxide (H ₂ O ₂ ) -treated feathers were higher and enzyme-treated feathers were lower.

There was no significant difference in mortality between the groups.

When comprehensively reviewing the above results, it was found that when the 5% by weight of feather powder or feather powder decomposition products were added to the control, the overall weight gain was lowered. This result is due to the poor digestibility and amino acid composition of the feathers and feather powder could not balance the energy / protein ratio. However, the initial growth rate of feather powder + pyridoxine added group seems to be due to the effect of pyridoxine added at 10 times the requirement of the National Research Council (NRC, 1994).

Increasing the growth rate due to the pyridoxine, pyridoxine acts as a coenzyme in decarboxylation, transamination, desulfhydration, etc. during the amino acid metabolism process, and is also involved in the oxidation of amines, phosphorylation of muscles and transport of amino acids (Leeson and Summers, 2001), in particular methionine It can be inferred to be due to the role of coenzyme in the process of synthesizing taurine from cystine. Therefore, it may have helped to metabolize excess protein by adding 5% by weight of feather powder, especially as a result of increased taurine synthesis in the body.

As taurine affects the growth of animals in the early stage of growth, it is inferred that by supplying a large amount of pyridoxine to cow powder, the most raw material of livestock feed, cysteine increases taurine content and improves the initial growth rate of broilers. Could. On the other hand, when the feather powder was used with hydrogen peroxide (H ₂ O ₂ ) or enzyme, there was no improvement effect on the weight gain, but the enzyme-treated feather powder was found to improve feed requirements.

Example 5 Sample Collection

After the end of the specification test (35 days of age), each of the 10 males and females close to the average weight of each treatment was selected for each treatment.

Test Example 5. Taurine Analysis

The taurine analysis was based on the analysis method of Paola Zunin et al. (1999) as in Test Example 2. The unit of the obtained result was represented by (micro | micron | gram) / g and fresh matter.

The result by the said test example 5 is shown to FIG. 4, 5, 6. FIG.

4, 5, and 6, there was a significant difference (P <0.01) between the treatments of the leg muscles and the breast muscles, but there was no significant difference in the liver.

As shown in Figure 4, the leg muscles were higher in the taurine content of the feather powder and feather digester added groups compared to the control group, 88% in the feather powder + pyridoxine addition group, 82% in the hydrogen peroxide (H ₂ O ₂ ) -treated feather powder There was an increase of 47% in the fractions and 14% in the enzyme-treated feathers.

As shown in FIG. 5, the chest muscle was the highest in the addition of feather powder + pyridoxine, but was 15% higher than the control.

As shown in FIG. 6, the woowoo powder + pyridoxine addition group was the highest in the liver, but there was no significant difference in the treatment section.

The inventors added pyridoxal or pyridoxalamine instead of pyridoxine based on the fact that pyridoxine added to the present invention is a precursor of coenzyme pyridoxalic acid together with pyridoxal and pyridoxamine as vitamin B ₆ . It could be inferred that the same result would be obtained even if the broilers were rejected.

Example 5 Sensory Test

In this example, the leg and chest samples were performed at the Chung-Ang University Department of Food and Nutrition for 5 males and 5 females per repetition. The test materials were prepared in a raw material and oven and broiled at 200 ° C. for 20 minutes, and the sensory tests were performed by dividing them by gender and parts. The evaluation items included aroma, color, off-flavor, juice purge, and overall acceptability in fresh meat, and aroma in cooked meat. ), Flavor, juiciness, tenderness of meat, and overall acceptability.

The sensory test was conducted once for each material, and the sensory test panel was conducted for 50 untrained consumers. In order to identify consumer's acceptance of functional chicken, the Hedonic Scale Acceptance Test is conducted to find out the sensory attributes that are considered important for determining palatability, while the scale of the scale to be used is 5 points. I used 5 points for very good and 1 point for very bad. The reason for selecting the degree of juice exudation and juiciness as an evaluation attribute is that the quality of the chicken meat is desirable in terms of quality and preference, not in a dry state, but in water holding capacity. The higher the score, the higher the water retention capacity. Juicyness of cooked chicken was evaluated based on the amount of water produced by chewing the ingredients 8 to 9 times.

In addition, when broiling chicken, the flavor and flavor were separated and evaluated in order to evaluate the unique aroma and taste produced from the meat. will be.

The results for Example 5 are shown in Tables 5, 6, 7, and 8 below.

Sensory test result (male) of chest muscle and leg muscle of fresh meat Pectoral muscles Leg muscle F-value T1 T2 T3 T4 T5 Average T1 T2 T3 T4 T5 Average Flavor 3.76 ^ab 4.00 ^a 3.38 ^ab 4.23 ^a 4.30 ^a 3.93 3.00 ^b 3.46 ^ab 4.15 ^a 3.84 ^ab 3.69 ^ab 3.63 2.10 ^* Meat color 2.84 3.15 3.00 2.07 2.53 2.72 3.07 3.00 3.07 3.38 3.61 3.23 1.72 Displeasure 2.76 3.07 2.61 3.00 3.07 2.90 2.15 2.61 2.92 2.92 2.61 2.64 0.42 Juicy exudation 3.76 ^a 3.38 ^ab 2.76 ^bc 2.38 ^c 3.53 ^ab 3.16 3.00 ^abc 2.69 ^bc 3.00 ^abc 3.00 ^abc 3.07 ^abc 2.95 1.95 ^* Full preference 3.30 3.46 2.76 2.76 3.30 3.12 2.92 2.84 3.15 3.53 3.53 3.19 1.31 ¹ T1; Control T2; Feed supplemented with 5% by weight of feather, T3; 5% by weight of feather powder + 21 mg / kg pyridoxine feed, T4; 5% by weight of hydrogen peroxide-treated feather powder feed, T5; Feed containing 5% by weight of enzyme-treated feather powder. Treatments with different superscripts differ significantly at P <0.05.

Sensory test results of breast and leg muscles of fresh meat (female) Pectoral muscles Leg muscle F-value T1 T2 T3 T4 T5 Average T1 T2 T3 T4 T5 Average Flavor 3.84 3.84 3.92 4.00 3.84 3.89 4.07 3.84 4.07 3.76 3.38 3.82 0.36 Meat color 2.92 ^abc 1.76 ^a 2.53 ^bca 2.38 ^ca 3.07 ^abc 2.53 3.61 ^a 3.23 ^abc 3.53 ^ab 2.46 ^ca 2.53 ^bca 3.07 3.27 ^*** Displeasure 2.92 2.84 3.00 3.00 3.00 2.95 3.07 2.84 2.92 2.84 2.38 2.81 0.17 Juicy exudation 3.53 ^ab 3.15 ^bc 3.46 ^ab 4.23 ^a 3.61 ^ab 3.60 3.15 ^bc 3.07 ^bc 2.92 ^bc 2.84 ^bc 2.38 ^c 2.87 2.92 ^** Overall desirability 3.23 3.15 3.30 3.53 3.53 3.35 3.53 3.23 3.53 3.23 2.76 3.26 0.78 ¹ T1; Control T2; Feed supplemented with 5% by weight of feather, T3; 5% by weight of feather powder + 21 mg / kg pyridoxine feed, T4; Feed supplemented with 5% by weight of hydrogen peroxide-treated feather powder, T5; Feed containing 5% by weight of enzyme-treated feather powder. Treatments with different superscripts differ significantly at P <0.05.

Sensory test result (female) of chest muscle and leg muscle of cooked meat Pectoral muscles Leg muscle F-value T1 T2 T3 T4 T5 Average T1 T2 T3 T4 T5 Average Flavor 2.53 2.93 2.86 2.93 2.66 2.78 3.00 2.26 2.86 2.66 2.60 2.68 0.52 zest 2.40 2.86 3.06 2.93 2.40 2.73 2.60 2.53 2.80 2.53 2.60 2.61 0.76 Succulent 2.26 ^bc 2.60 ^ab 3.00 ^a 2.33 ^abc 2.20 ^bc 2.48 1.86 ^c 1.60 ^c 1.80 ^c 1.60 ^c 1.86 ^c 1.74 3.95 ^*** Softness 2.93 ^a 2.66 ^a 3.13 ^a 2.80 ^a 2.73 ^a 2.85 1.60 ^b 1.66 ^b 1.66 ^b 1.73 ^b 1.73 ^b 1.68 6.68 ^*** Overall desirability 2.60 ^abcd 2.86 ^ab 2.86 ^ab 3.00 ^a 2.80 ^abc 2.82 2.00 ^d 2.13 ^cd 2.26 ^bcd 2.13 ^cd 2.26 ^bcd 2.16 3.00 ^{** 1} T1; Control T2; Feed supplemented with 5% by weight of feather, T3; 5% by weight of feather powder + 21 mg / kg pyridoxine feed, T4; Feed supplemented with 5% by weight of hydrogen peroxide-treated feather powder, T5; Feed containing 5% by weight of enzyme-treated feather powder. ** Treatments with different superscripts differ significantly at P <0.01. *** Treatments with different superscripts differ significantly at P <0.01.

Sensory test result (male) of chest muscle and leg muscle of cooked meat Pectoral muscles Leg muscle F-value T1 T2 T3 T4 T5 Average T1 T2 T3 T4 T5 Average Flavor 2.80 2.60 2.20 2.33 2.73 2.53 2.80 2.53 2.53 2.66 2.73 2.65 0.46 zest 2.46 2.46 2.13 2.40 2.40 2.37 3.06 2.60 2.66 2.80 3.00 2.82 1.22 Succulent 2.06 2.33 2.53 2.53 2.60 2.41 2.00 2.13 1.86 1.80 1.73 1.90 1.38 Softness 2.53 ^ab 2.46 ^ab 2.93 ^a 3.00 ^a 3.00 ^a 2.78 2.20 ^abc 2.06 ^bc 1.40 ^c 1.40 ^c 1.46 ^c 1.70 5.66 ^*** Overall desirability 2.53 2.66 3.00 2.86 2.40 2.69 2.60 2.40 2.00 2.00 2.20 2.24 1.59 ¹ T1; Control T2; Feed supplemented with 5% by weight of feather, T3; 5% by weight of feather powder + 21 mg / kg pyridoxine feed, T4; Feed supplemented with 5% by weight of hydrogen peroxide-treated feather powder, T5; Feed containing 5% by weight of enzyme-treated feather powder. *** Treatments with different superscripts differ significantly at P <0.01.

As shown in Tables 5 and 6, males were better than females in the case of flavor and flesh color in the case of fresh meat, and females had increased discomfort, juice exudation, and overall desirability. .

In the case of leg muscles of fresh meat, females showed higher flavor, displeasure, and overall desirability than males.

As shown in Tables 7 and 8, all items of flavor, flavor, juiciness, softness, and general preference were found to be higher in males than in females, and in the case of leg muscles, the five items were higher than females in males. Appeared.

In a comprehensive review of the above results, the overall desirability of fresh meat was better than that of hens in terms of gender, but no significant difference was observed. In the case of flesh color, the cock was better than the hen in breast and leg muscles. The score was better, the juicy effusion was better in hen breast, and the leg flavor of rooster was highest in FM + pyridoxine supplement.

In comparison, the breast muscles were better than the leg muscles in both hens and roosters. This trend shows that hens are consistently better than roosters.

In the case of cooked meat, there was no significant difference according to gender in terms of gender, but in the case of rooster, breast muscle tended to have better scores than hens and leg muscles in hens.

In comparison, the chest muscles and leg muscles were compared and evaluated. The breast muscles in cocks were better evaluated in all attributes, including overall desirability. In particular, significant differences were observed between the treatments in the juiciness and tenderness of the meat. This tendency was similar in hens, indicating that the breast muscles of the hens scored higher than the leg muscles in terms of overall desirability, juiciness, and softness of the flesh.

As a result of the above, the inventors added pyridoxine to the feed added with 5% by weight of feather powder to increase the initial gain and effectively strengthen taurine in the leg, especially the leg, and by sex, site, There were differences according to cooking status and there was no constant tendency, but it was proved that feather powder-pyridoxine addition group was relatively good among feather powder or feather digest products.

The present invention is not limited to the technical spirit described in the specific embodiments, test examples, drawings or diagrams described above, and the general knowledge in the technical field to which the present invention belongs without departing from the gist of the present invention as claimed in the claims. Of course, any person having a variety of modifications can be made, and such changes are within the scope of the claims.

As described in detail above, the present invention relates to a method for producing taurine-containing poultry, characterized in that it is specified as feed containing cow's powder, taurine content in poultry, specified as untreated cow powder in the late broiler It was confirmed that the increase in taurine, especially in the leg muscles.

In another aspect, the present invention has the effect of increasing the taurine content in the leg muscles, breast muscles, liver of broilers that were specified as feed supplemented with feather powder and pyridoxine in the early broiler.

In addition, the present invention has the effect of increasing the weight gain of broiler compared to the feed containing the cow's milk powder and pyridoxine.

Claims (4)

delete A method for producing taurine-enhanced chicken meat, characterized in that it is specified as a feed containing feather powder and pyridoxine. The method of claim 2, The feed is a method for producing taurine-enhanced chicken meat, characterized in that the feed is characterized by a feed containing 5% by weight of the cow powder and pyridoxine 21mg / kg. Chickens with increased taurine produced by the method of claim 2 or 3.

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