KR100638352B1 - Extract of Gromwell - Google Patents

Abstract

The present invention relates to a licorice extract, and more particularly, in the case of atopic dermatitis or dryness in which the fat layer structure of the skin epidermal barrier is damaged and the epidermis proliferates, the epidermis is suppressed to prevent epidermal hyperproliferation and prevents the loss of moisture of the epidermis. Relates to an extract.

According to the present invention, it is possible to treat atopic dermatitis or dryness inexpensively and simply by using the Korean herbaceous extract which is a domestic plant resource.

Self-Contained, Atopic Inflammation

Description

Herbal Extracts {Extract of Gromwell}

1 is a normal control group ingesting black currant oil (BCO) or borage oil (BO) for 10 weeks, unsaturated fatty acid-deficient guinea pigs ingesting coconut oil (HCO) for 10 weeks, or 8 weeks after coconut oil intake. The state has been shown to reduce trans epidermal water loss (TEWL) through the skin of guinea pigs fed black currant oil (HBCO), borage oil (HBO), organic herb extract (GO), or hot boiled extract (GW). It is a result of a measurement.

FIG. 2 is a view over time of epidermal growth of guinea pigs in each treatment group as in FIG. 1. FIG.

Fig. 3 shows the skin thickness of guinea pigs in each treatment group.

Fig. 4 shows the reaction for detecting [3H] thymidine into epidermal DNA of guinea pigs in each treatment group.

5 and 6 show the unsaturated fatty acid (PUFA) composition of phospholipids and ceramides in the epidermis of guinea pigs of each treatment group.

Figure 7 shows altered intracellular levels of 13-HODE in the epidermis of guinea pigs of each treatment group.

8 shows altered intracellular levels of 15-HETrE in the epidermis of guinea pigs of each treatment group.

The present invention relates to a licorice extract, and more particularly, in the case of atopic dermatitis or dryness in which the fat layer structure of the skin epidermal barrier is damaged and the epidermis proliferates, the epidermis is suppressed to prevent epidermal hyperproliferation and prevents the loss of moisture of the epidermis. Relates to an extract.

The epidermis, the outermost layer of the skin, protects the body from the outside. The stratum corneum, the uppermost layer of the epidermis, is the epidermal barrier, which inhibits evaporation and loss of the epidermis, prevents the epidermis from drying out, and prevents bacteria, fungi, and viruses. It acts as the first line of human defense mechanism to prevent skin invasion.

The stratum corneum has a layered structure in which fats are placed between the keratinocytes and the bricks and the plaster therebetween.

The epidermal barrier is a mixture of lipids such as phospholipids, cholesterol, and ceramides. These lipids are linolenic acid (LA: 18: 2n-6) and arachidonic acid (AA: 20: 4n-), which are representative unsaturated fatty acids in the epidermis to maintain normal layered structure. 6), γ-linolenic acid (GLA: 18: 3n-6), which is mainly bound to the phospholipids in the basal epidermis and migrates to ceramides as keratinocytes differentiate to form acyl ceramides.

On the other hand, atopic dermatitis, in which the lipid layer structure of the epidermal barrier is damaged, exhibits symptoms of dryness, hyperproliferation and inflammation of the epidermis due to moisture evaporation and infection through the epidermis.

Chronic skin diseases, including atopic dermatitis and epidermal dryness, abnormal proliferation and inflammation, account for more than 5% of outpatients in dermatology in Korea. Current treatments include steroid preparations or UV therapy to inhibit DNA synthesis. However, these therapies focus on inhibiting cell growth and inhibiting inflammation, but these therapies involve multiple side effects and are difficult to expect fundamental healing, and are only meant to prolong inflammation and prevent relapses and to prolong remission. It was urgent to develop a therapeutic agent that can be used for a long time without being involved.

In the stratum corneum of patients with atopic dermatitis, levels of various lipids, including unsaturated fatty acids, have been reported, and atopic dermatitis treatment through dietary addition of unsaturated fatty acids was attempted. Among these, γ-linolenic acid (GLA: 18: 3n-6) was studied. Intake of Evening Primrose Oil ( Oenothera biennis ) and Borage Oil ( Borago officinalis ), the main source of oil), has been shown to be clinically superior in the treatment of the disease.

In terms of fatty acid metabolism of the epidermis, γ-linolenic acid (GLA: 18: 3n-6), unlike other tissues in the body, is not recognized in the linkage pathways of n-6 unsaturated fatty acids such as LA-AA. It is explained by the strong activity of the absence and elongation enzymes. In other words, GLA can be converted to dihomo-γ-linolenic acid (DGLA: 20: 3n-6) due to the strong activity of the long chain enzyme in the epidermis, but not to AA, which requires the Δ5 unsaturated process, and LA is absent from the Δ6 unsaturated enzyme. It is not possible to switch to GLA.

In the epidermis, γ-linolenic acid (GLA: 18: 3n-6) is converted to dihomo-γ-linolenic acid (DGLA: 20: 3n-6) and not converted to AA by the strong activity of the long chain enzyme, whereas As a substrate of the sanoid, cyclooxygenase (COX) is used to form phosphaglandin E1 (PGE1), and 15-lipoxygenase (15-LOX) is used to form 15-HETrE (15-hydroxy-8,11). , 14-Eicosatetraenoic acid) are metabolites that have the effect of inhibiting epidermal aberration and inflammation, and their activity efficiency is 20 times higher than that of other unsaturated fatty acids.

Γ-linolenic acid (GLA: 18: 3n-6), which is growing in demand as a pharmacological fatty acid, is known to be distributed in several plant species, including Boraginaceae, Scrophulariaceae and Saxifraceae. These major resource plants are produced in the Americas and New Zealand. Therefore, it is difficult to use and expensive because it depends entirely on imports in Korea.

On the other hand, is self-inflicted domestic plant resources (saengyakmyeong: Radix Lithospermi) is exhausted: Behold a perennial plant of the (scientific Lithospermum erythrorhizon) Jiyugaoka against inde maintain derived from Borago officinals seed, as the roots of the fig (Gromwell) Classico Nin (Shikonin ) And Acetylshikonin together with other components such as polysaccharides, Lithsperman, etc., and it is used to treat boils, burns, eczema, etc. due to blood circulation promoting action, detoxification action, antibacterial and anti-inflammatory action. It is known as a commonly used herbal medicine.

In addition, Korean Patent Laid-Open Publication No. 2000-0038904 discloses that the herbaceous plant promotes angiogenesis of wound and wound surface cells, and has anti-inflammatory and wound healing promoting effects.

Thus, the present inventors have searched for GLA-containing plants from domestic resource plants for the treatment of atopic dermatitis. I noticed that I used it as a disinfectant on my back. As a result of the analysis of the grass, it was found that 0.3 mg / g dry weight of GLA was detected in the grass.

The present invention was devised to solve the above problems, and an object thereof is to provide a self-extracting extract as a treatment for atopic dermatitis or dryness in which the fat layer structure of the skin epidermal barrier is damaged and the epidermis is abnormally multiplied.

Other objects and advantages of the invention will be described below and will be appreciated by the practice of the invention. In addition, the objects and advantages of the present invention can be realized by means and combinations indicated in the claims.

In order to achieve the above object, the present invention, in the case of atopic dermatitis or dryness in which the fat layer structure of the skin epidermal barrier is damaged and the epidermis proliferates, the epidermal hyperplasia inhibits epidermal hyperplasia and prevents water loss of the epidermis. To provide.

The extract was added to 10 times the amount of Polts solution [chloroform / methanol (2: 1 v / v)] and 2 times 0.1M KCl, and then centrifuged (800 xg, 10 minutes) to pulverized liquor, It may be in powder form prepared by concentrating and freeze drying for 72 hours.
In addition, the extract may be in the form of a powder prepared by adding 20 times the amount of distilled water to the pulverized licorice and heating the hot water for 2 hours in a boiling water extractor, concentrated under reduced pressure and freeze-dried for 72 hours.

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Licorice extract according to the present invention can be used in combination with other drugs useful for the treatment of atopic dermatitis or dryness. The individual components of such a mixture may be administered separately at different times during treatment or simultaneously or once in divided forms in a mixed form. Accordingly, the invention is to be understood as encompassing all areas of concurrency or replacement and the term “administration” is to be interpreted accordingly. The range of mixing the herbaceous extract of the present invention with other drugs useful for the treatment of atopic dermatitis or dryness includes all mixing with pharmaceutical compositions useful for the treatment of atopic dermatitis or dryness.

The herbaceous extract of the present invention can be administered orally in the form of tablets, capsules (each of which is a sustained release or secretory formulation after a certain time), pills, powders, particulates, elixirs, tinctures, suspensions, syrups and emulsions.

Prescription dosages using the compounds of the present invention take into account various factors such as type, species, age, weight, sex, and the patient's health condition, the severity of the condition to be treated, the route of administration, and the function of the patient's kidney or liver. Is selected. Ordinary physicians, veterinarians or clinicians can easily determine and prescribe the pharmacologically effective amount of the drug required to prevent, counter or prevent the development of the condition.

The oral dosage of the present invention used for the indicated effect is between about 0.01 mg (mg / kg / day) to about 100 mg (mg / kg / day) per kg of weight per day, preferably per kg of weight per day It is between about 0.01 mg (mg / kg / day) to about 10 mg (mg / kg / day), more preferably from 0.1 to about 5.0 mg / kg / day. For oral administration, the compositions contain 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, and 500 mg active factors for symptomatic dose adjustments to patients to be treated. It is preferably provided in the form of a containing syrup. Drugs typically contain from about 0.01 mg to about 500 mg of active factor, and preferably from about 1 mg to about 100 mg of active factor. Intravenously, the most preferred dose is 0.1 to 10 mg / kg / min during continuous infusion. Preferably, the licorice extract of the present invention may be taken once a day, or divided into two, three or four times a day to administer a daily dose. In addition, the licorice extract of the present invention may be administered in nasal form via topical using a suitable nasal carrier.

The herbaceous extract of the present invention may be in the form of an active factor, and is a pharmaceutically suitable diluent, excipient or carriers selected herein for dosage forms of oral tablets, capsules, elixirs, syrups, etc. Is administered by typical pharmaceutical techniques.

For example, for oral administration in the form of a tablet or capsule, the active drug ingredient may be an oral, nontoxic, pharmaceutically acceptable, lactose, starch, sucrose, glucose, methyl cellulose, magnesium stearate, diphosphate Compound with inert carriers such as calcium, calcium sulfate, mannitol, sorbitol, and the like; For oral administration in liquid form, the active drug ingredient may be combined with an inert carrier such as oral, nontoxic, pharmaceutically acceptable, ethanol, glycerol, water, and the like.

In addition, if necessary, suitable binders, lubricants, dispersants and coloring agents may also be added to the mixture. Suitable binders include natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic rubbers such as corn sweeteners, acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycols, waxes, and the like. . Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Dispersants include, but are not limited to, starch, methylcellulose, agar, bentonite, xant rubber and the like.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms or words used in the specification and claims should not be construed as having a conventional or dictionary meaning, and the inventors should properly explain the concept of terms in order to best explain their own invention. Based on the principle that can be defined, it should be interpreted as meaning and concept corresponding to the technical idea of the present invention.

Therefore, the embodiments described in the specification and the drawings shown in the drawings are only the most preferred embodiments of the present invention and do not represent all of the technical idea of the present invention, various equivalents that may be substituted for them at the time of the present application It should be understood that there may be water and variations.

<Example>

1. Experimental Animal

The experimental animals purchased 50 male guinea pigs of two-week-old Hartley species (Samtako, Korea) and were adapted to a solid blended diet for 1 week before breeding on the experimental diet. Coconut oil was deficient in unsaturated fatty acids for 8 weeks to induce epidermal hyperproliferation, and divided into groups of egg mass according to body weight to compare the inhibitory effects of induced epidermal hyperproliferation. It was bred for two weeks with an experimental diet containing. The diet was fed a daily dose of 40g and freely ingested water containing 0.05% of vitamin C. Experimental animals were reared one by one in stainless steel cages for 10 weeks. The temperature of the feeding room was maintained at 22 ± 1 ℃ and 60 ± 5% of humidity, and the photoperiod and dark cycle were adjusted to 12 hours daily.

2. Licorice Extract

Licorice used in the experiment was harvested and dried in 2001 in Yeongju, Gyeongsangbuk-do, and prepared both hot water extract and organic extract.

After adding 10 times the amount of Polts solution [Chloroform / Methanol (2: 1 v / v)] and 2 times 0.1M KCl, the resultant was centrifuged (800 xg, 10 minutes), and the lower layer was concentrated under reduced pressure. Lyophilization for a period of time to prepare an organic extracted porcelain powder in powder form.

However, because the intake of sage, which is derived from oriental medicine and folk medicine and used for the treatment of various skin diseases, is made by conventional scalding methods, sorghum powder extracted from boiling water was also prepared in order to apply realistic intake method of sage. That is, 20 times the amount of distilled water was added to the pulverized licorice, and the resultant was heated for 2 hours in a boiling water extractor.

After extraction and freeze-drying process, the yields of the organic extract and hot water extract in powder form were 23.5% and 15%, respectively, based on the initial sample weight.

3. Dietary composition

The dietary composition of each experimental group is shown in Table 1, and the dietary fat was fixed at 6% of the dietary weight according to the Guinea Pig basic dietary standard.

Table 1

Unit: g / Kg dry diet

Furtherance  Dietary group BO / HBO BC / HBC HCO GO GW Coconut Keep - - 60 60 60 Borage 60 - - - - Black Currant Oil - 60 - - - casein 300 300 300 300 300 Corn starch 257.98 257.98 257.98 246.23 250.48 Sucrose 100 100 100 100 100 Cellulose 130 130 130 130 130 Mineral mixtures 60 60 60 60 60 Vitamin mixtures 40 40 40 40 40 Baby 20 20 20 20 20 DL-Methionine 2 2 2 2 2 Potassium acetate 25 25 25 25 25 Magnesium oxide 5 5 5 5 5 Zinc carbonate 0.02 0.02 0.02 0.02 0.02 Organic Organic Extract - - - 11.75 - Jacho Hot Water Extract - - - - 7.5

Hydrogenated coconut oil (HCO) was added to induce epidermal hyperproliferation, and borage oil, blackcurrant oil or turmeric hot water and organic extracts were added to the diet for inhibition of induced epidermal hyperproliferation. Induction of unsaturated fatty acid deficiency and epidermal hyperproliferation by dietary feeding of coconut oil for 8 weeks was characterized by accumulation in the epidermis of etaosatrienoic acid (ETA) (20: 3n-9) (biochemical indicator of unsaturated fatty acid deficiency) using gas chromatography. It was previously validated by histological analysis.

After eight weeks of feeding coconut oil to the diet, the divided experimental groups boiled coconut oil (HCO group), borage oil (HBO group), blackcurrant oil (HBC group), or 5% of the total diet weight. GW group) or organic extract (GO group) was mixed with the diet and fed for 2 weeks.

The weight difference of the dry starch diet resulting from the use of lyophilized licorice extract was adjusted by subtracting the corn starch weight.

The experimental group (HCO group), which supplies coconut oil for 8 + 2 weeks, served as a negative control for epidermal hyperproliferation inhibition. In addition, the experimental group (BO group: BC group) to supply borage or black currant oil for 8 + 2 weeks was a normal control. The fatty acid composition (g / 100g total fatty acid) of each fat and oil was shown in Table 2.

[Table 2]

fatty acid BO BC HCO 8: 0 7.1 10: 0 6.5 12: 0 51.2 14: 0 17.5 16: 0 8.7 8.0 8.8 18: 0 3.6 5.7 7.1 18: 1n-9 16.6 15.3 18: 2n-6 36.2 45.0 18: 3n-3 - 12.0 18: 3n-6 22.3 14.0

4. Epidermal proliferation measurement

1) Histological Evaluation

In order to visually measure the extent of epidermal growth, epidermal tissue of 4 mm 2 was taken from the back and cut to 6 μm, and then fixed and stained with H & E (hematoxylin & eosin) and observed under a microscope.

2) Thymidine Incorporation

Since the increase of DNA is an indicator of cell proliferation, the degree of epidermal proliferation was measured by incorporation of [3H] thymidine, a DNA structure. Epidermal tissue was collected by a 6 mm 2 biopsy punch, incubated with [3 H] thymidine (0.1 Ci / ml; 5 ml) in 1 x DMEM for 3 hours at 37 ° C and liquid nitrogen to inhibit DNA synthesis. Stored in. 2 ml of 0.5 M NaOH was added to the thawed epidermal tissue and heated at 95 ° C. for 30 minutes to release the DNA from the tissue, and a portion of it was added to a cellulose filter treated with 1% trichloroacetic acid to measure radioactivity with a scintillation tube. A portion of the DNA free solution was taken and the amount of DNA was quantified using absorbance measurement at 280 nm and the thymidine detection reaction was expressed as [3H] cpm Thymidine / mg DNA.

5. Epidermal Lipid Fraction and Fatty Acid Analysis

4 cm 2 of guinea pig epidermis was pulverized with a polytron (PT-2100, Kinematica, Swiss), and lipid was extracted using a Polz solution. It was dried with nitrogen gas and dissolved in 100 μl of a solution of Polts, and then added to HP-TLC (high performance thin layer chromatograph: sillica gel 60, 200 μm thickness, Whatman, USA), and the primary developer chloroform: methanol: acetone (76 : 20: 4, v / v / v), secondary developer chloroform: methanol: acetone (80:10:10, v / v / v) and tertiary developer chloroform: ethyl acetate: ethyl ether: methanol (76 : 20: 6: 2, v / v / v / v) was used to fractionate phospholipids and ceramide lipids. Each separated lipid was scraped from the thin layer and then the lipids were extracted with a Polts solution and the fatty acid was methylated using methanol containing 6% HCl. Fatty acid methyl ester (FAME) is a gas chromatography (5890 series II Gas) equipped with an Omegawax 320 Fused Silica Capillary column (30m x 0.32mm id, 0.25μm film thickness, Supelco, USA) chromatograph, Hewlett packard, USA) and analyzed the fatty acid profiles of each fraction. The analytical conditions were maintained at 240 ° C. for 10 minutes by maintaining the temperature of the oven at 140 ° C. for 5 minutes at a 250 ° C. inlet and a 260 ° C. flame ionization detector. At this time, helium gas (carrier gas) was flowed at a flow rate of 3 ml / min, and 1 μl of sample was injected under the condition that the split ratio was 20: 1.

6. Analysis and Measurement of Unsaturated Fatty Lipoxygenase Metabolites

RIPA buffer (20 mM Tris-HCl, pH 3.0, 150 mM NaCl, 10 mM NaPO4, 10% glycerol, 100M Na3VO4, 100M ammonium molybdate, 1% NP-40, 0.1% SDS) was added to 4 cm2 of guinea pig epidermis and triturated with polytron. It was. The same amount of Polts solution was added and centrifuged (3000 rpm, 5 minutes). The lower layer was taken, dried over nitrogen gas and dissolved in methanol.

It was injected into HPLC (Waters 1500 series HPLC: 1525 binary HPLC pump, 2487 Dual Absorbance detector, U.S.A.) to compare and analyze unsaturated fatty acid metabolites. The ODS 18 column (reverse phase, 25 cm x 4.6 μm id, Beckman, USA) was analyzed at 237 nm and the solvent (flow rate: 1 mL / min) was H2O containing methanol: 0.08% acetic acid (76: 26, v / v).

7. Statistical Analysis

 The experimental results of the present invention are expressed as the mean value and the standard deviation, and the significance between the experimental groups using the SAS program was verified by one-way ANOVA and compared by Duncan's multiple range test at p <0.05 level.

<Result>

1. Effect of Dietary Supplementation of Swine on Epidermal Growth (TEWL, H & E, Ki67, thymidine)

1 is a normal control group ingesting black currant oil (BCO) or borage oil (BO) for 10 weeks, unsaturated fatty acid-deficient guinea pigs ingesting coconut oil (HCO) for 10 weeks, or 8 weeks after coconut oil intake. The state has been shown to reduce trans epidermal water loss (TEWL) through the skin of guinea pigs fed black currant oil (HBCO), borage oil (HBO), organic herb extract (GO), or hot boiled extract (GW). It is a result of a measurement. The result is mean ± SEM (n = 10).

As a result of measuring trans epidermal water loss (TEWL), the average loss of moisture in the GO group fed with organically-derived licorice powder was similar to BC, BO, and HBO groups, compared to HCO, HBC, and GW groups. Although low, there was no significant difference among the groups in the statistical analysis (see Table 3).

Table 3

Dietary group TEWL BC 8.8 ± 0.9 BO 9.3 ± 1.2 HCO 10.1 ± 1.4 HBC 11.6 ± 2.5 HBO 9.3 ± 0.9 GO 9.6 ± 1.8

FIG. 2 is a view over time of epidermal growth of guinea pigs in each treatment group as in FIG. 1. FIG. The arrow indicates the basal layer of the epidermis and epidermal growth is related to the epidermal thickness from the arrow to the top.

FIG. 3 is the thickness of the skin of the guinea pigs of the same treatment groups as in FIG. The result of Fig. 2 is numerically calculated, and the result is mean ± SEM (n = 10). The mean of the other characters differs by p <0.0001. Epidermal thickness hyperplasia of the HCO group can be seen. Dietary supplementation of S. aureus inhibited epidermal hyperproliferation, especially in GO group fed with S. aureus extract, and significantly reduced epidermal hyperproliferation. Similar to the BO group. Epidermal hyperproliferation was also inhibited in the HBC and HBO groups but thicker than the BO group.

Histological evaluation of epidermal proliferation using H & E staining revealed that the epidermal layer was thickest in the HCO group fed dietary coconut oil (see Table 4).

This is because the unsaturated fatty acid deficiency destroys the water barrier of the skin tissue and leads to excessive proliferation and keratinization of epidermal cells with excessive production of sterol-ester. Dietary supplementation of S. aureus inhibited epidermal hyperproliferation, especially in GO group fed with S. aureus extracts ( P <0.0001). Epidermal hyperproliferation inhibitory effect was similar to that of the BO group. Epidermal hyperproliferation was also inhibited in the HBC and HBO groups, but the epidermis was thicker than the BO group.

Table 4

Dietary group Skin layer thickness (㎛) BC 29.4 ± 4.4 ^abc BO 15.7 ± 1.9 ^dc HCO 45.4 ± 5.2 ^a HBC 22.5 ± 2.5 ^bcd HBO 38.8 ± 6.3 ^ab GO 9.2 ± 1.2 ^d GW 15.3 ± 6.1 ^cd

Fig. 4 shows the reaction for detecting [3H] thymidine into epidermal DNA of guinea pigs of each treatment group as in Fig. 1. Results are mean ± SEM. The mean of the other characters differs by p <0.05.

[3H] thymidine detection reaction was used to biochemically assess the extent of epidermal proliferation in each experimental group (see Table 5).

Table 5

Dietary group cpm / μg DNA BC 231.1 ± 45.8 ^ab BO 233.6 ± 41.3 ^ab HCO 301.5 ± 46.1 ^a HBC 270.8 ± 46.9 ^a HBO 224.8 ± 20.1 ^ab GO 104.2 ± 4.3 ^c GW 143.8 ± 12.3 ^bc

The thymidine incorporation rate was the highest in the HCO group, indicating that epidermal hyperproliferation is in parallel with increased DNA synthesis. The thymidine incorporation rate of the HBO group was similar to the BC and BO groups. The thymidine incorporation rate was the lowest in the GO group after dietary administration of S. aureus extract, and the levels were significantly lower than those of the BC and BO groups. The thymidine incorporation rate after dietary feeding (GW group) of scorched extracts was also similar to that of GO group. In addition to unsaturated fatty acid deficiency diets, including linolenic acid, infants fed skim milk powder or enteral nutrition lacking lipids have been reported to result in epidermal dropout and epidermal hyperplasia with decreased growth. Was consistent with these. Induction of epidermal hyperplasia showed a tendency of suppression of proliferation by dietary supplementation of black currant oil, borage oil and soybean extract, but the effect of the organic extract was most significant. Combined histological and biochemical evaluations, dietary supplementation of organically extracted soybean powder is considered to be the most effective in suppressing epidermal hyperproliferation.

2. Effect of Dietary Supplementation of Licorice on Fatty Acid Composition of Epidermis

Linolenic acid levels in the HCO group were significantly lower than in the HBC and BO groups (see Table 6).

Table 6

The GO group fed dietary herb extracts increased linolenic acid content to a similar level as the HBO group. The content of linolenic acid after dietary supplementation (GW group) of the liquor hot water extract was similar to that after the diet of unsaturated fatty acid deficiency diet (HCO group), and there was a significant difference between the groups. The content of γ-linolenic acid in each group tended to be similar to that of linolenic acid. The GO and HBO groups had similar levels, and the GW and HCO groups showed similar levels.

5 and 6 show the unsaturated fatty acid (PUFA) composition of phospholipids and ceramides in the epidermis of guinea pigs of each treatment group as shown in FIG. Results are mean ± SEM. The mean of the other characters differs by p <0.05.

Lipids contained in the epidermis were divided into phospholipids (PL), ceramides (CE) and neutral lipids (NL), and the contents of LA and DGLA (g / 100g total fatty acids) were compared (see Table 7).

Table 7

 PUFA Fat Diet Group BC BO HCO HBC HBO GO GW 18: 2n-6 PL 9.0 ± 6.2 ^bc 10.5 ± 3.4 ^b 4.4 ± 2.4 ^c 8.1 ± 4.4 ^bc 10.0 ± 3.1 ^b 15.5 ± 4.0 ^a 9.4 ± 2.4 ^bc CE 9.3 ± 2.5 ^ab 8.6 ± 4.7 ^b 4.6 ± 3.0 ^b 8.5 ± 6.8 ^b 7.3 ± 6.0 ^b 14.6 ± 3.4 ^a 10.7 ± 3.8 ^ab NL 14.9 ± 5.6 ^ab 14.9 ± 7.7 ^ab 7.4 ± 3.8 ^b 10.0 ± 6.8 ^b 12.0 ± 8.6 ^ab 19.8 ± 6.5 ^a 8.7 ± 4.6 ^b 20: 3 n-6 PL 1.8 ± 0.9 ^ab 1.6 ± 1.3 ^ab 0.9 ± 0.8 ^ab 1.5 ± 1.4 ^b 1.3 ± 0.8 ^b 2.7 ± 0.8 ^a 2.8 ± 1.1 ^a CE 1.8 ± 0.7 ^ab 2.3 ± 0.9 ^a 0.7 ± 0.3 ^ab 1.4 ± 0.4 ^bc 1.2 ± 0.4 ^bc 2.4 ± 1.5 ^a 1.1 ± 0.5 ^bc NL 1.7 ± 1.6 2.7 ± 1.8 1.0 ± 0.4 1.3 ± 1.1 1.5 ± 1.0 1.8 ± 0.9 1.5 ± 0.6

In the GO group, the levels of LA in phospholipids, ceramides, and neutral lipids were the highest, which were higher than those in the BC and BO groups. In addition, the level of DGLA contained in ceramide was also the highest in the GO group. DGLA levels contained in neutral lipids did not show any significant differences among the groups.

3. Effect of Dietary Supplementation of Licorice on the Production of Unsaturated Fatty Acid Lipoxygenase Metabolites in Epidermis

FIG. 7 shows altered intracellular levels of 13-HODE in the epidermis of guinea pigs of each treatment group as in FIG. 1. Results are mean ± SEM.

FIG. 8 shows altered intracellular levels of 15-HETrE in the epidermis of guinea pigs of each treatment group as in FIG. 1. Results are mean ± SEM. The mean of the other characters differs by p <0.05.

The production of 13-HODE (13-hydroxyoctadecadienoic acid) and 15-HETrE, the metabolites of 15-lipoxygenase, reflected the levels of linolenic acid and dihomo-γ-linolenic acid in phospholipids and ceramides (Table 5), respectively. See Table 8).

Table 8

Dietary group 13-HODE (μg / g protein) 15-HETrE (μg / g protein) BC 265.2 ± 75.2 87.5 ± 31.5 ^abc BO 259.5 ± 71.1 72.2 ± 24.5 ^ab HCO 168.4 ± 46.8 31.2 ± 8.9 ^c HBC 259.2 ± 20.5 61.1 ± 7.5 ^bc HBO 191.9 ± 52.9 49.6 ± 5.2 ^bc GO 338.5 ± 64.3 143.3 ± 13.7 ^a GW 300.9 ± 79.0 102.0 ± 12.0 ^ab

The mean of 13-HODE production was high in GO group, but there was no significant difference from other groups. In the HCO group, 15-HETrE production was the lowest. The amount of 15-HETrE produced was the highest in the GO group. This result suggests that the intake of organically extracted soybean powder can be developed into a food material that supplies γ-linolenic acid to the body more effectively than borage oil, blackcurrant oil and soybean hot water extract.

Induction of epidermal hyperproliferation by unsaturated fatty acid deficiency diet, followed by suppression of epidermal hyperproliferation by dietary supplementation of soy extract or blackcurrant oil and borage oil and linolenic acid (LA: 18: 2n-6), di-phosphate and ceramide The results of comparing and analyzing homo-γ-linolenic acid (DGLA: 20: 3n-6), 13-HODE, 15-HETrE, and ceramide production are summarized as follows.

1) Histological evaluation of epidermal proliferation and biochemical evaluation of epidermal proliferation using the thymidine detection reaction showed that the epidermal layer was thickened and the thymidine incorporation rate was the highest in the HCO group that induced hyperproliferation. In the HBC and HBO groups, the epidermal hyperproliferation was inhibited, but the hyperproliferation was most significantly reduced in the GO group fed with the organic extract.

2) The level of linolenic acid in total lipids of epidermis was similar to that of HBO group in GO group fed dietary extract. The content of linolenic acid in the GW group fed dietary water of Jacho hot water extract was similar to that of HCO group.

3) In comparison of the contents of LA and DGLA in phospholipids, ceramides and neutral lipids, the level of LA in phospholipids, ceramides and neutral lipids was the highest in the GO group, and the level of DGLA in ceramides was also the highest. .

4) In the GO group, 15-HETrE production was reported to be the metabolite of DGLA 15-lipoxygenase, which reported the anti-proliferative and anti-inflammatory physiological activities. The production of 13-HODE and 15-HETrE in HCO group was lower than that of normal control group (BCO group, BO group) and showed significant difference between groups.

Taken together, dietary supplementation of the organic extract with guinea pigs induced epidermal hyperproliferation induced dichomo-γ-linolenic acid in phospholipids and ceramides, a lipid component important for maintaining the epidermal barrier more significantly than black currant oil or borage oil. Increasing production of 15-HETrE inhibits epidermal hyperproliferation. On the other hand, the epidermal hyperproliferation inhibitory effect of the hot water extract of the soybean powder is lower than that of the soybean organic extract, but little by little.

According to the present invention, there is an advantage that it is possible to treat atopic dermatitis or dryness inexpensively and simply by using the Korean herbaceous extract, which is a domestic plant resource.

In other words, it is possible to develop self-contained and skin-related health functional foods as a resource that surpasses black currant oil or borage oil, which has been used as an epidermal hyperproliferation inhibitor.

Claims (6)

A dietary supplemental herbaceous extract that inhibits epidermal hyperproliferation by preventing epidermal hyperproliferation in the case of atopic dermatitis or dryness in which the fatty layer structure of the skin epidermal barrier is damaged and the epidermis proliferates. The method of claim 1, The extract was added to 10 times the amount of Polts solution [chloroform / methanol (2: 1 v / v)] and 2 times 0.1M KCl, and then centrifuged (800 xg, 10 minutes) to pulverized liquor, Licorice extract, characterized in that the powder form prepared by concentration and freeze-dried for 72 hours. The method of claim 1, The extract is a licorice extract, characterized in that the powder form prepared by adding 20 times the amount of distilled water to the pulverized porcelain, heat-preheated in a boiling water extractor for 2 hours, concentrated under reduced pressure and freeze-dried for 72 hours. delete delete delete

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