KR100588805B1 - Apparatus and Methods for Manufacturing micro-structure - Google Patents

Abstract

The present invention relates to an apparatus for manufacturing microstructures such as microwires and microtubes, microbeads, and a manufacturing method using the same. More particularly, the fabrication of the apparatus and the manufacture of the microstructures can be easily and economically performed. The present invention relates to a manufacturing apparatus capable of manufacturing structures of various diameters by controlling only the flow rate, and to a method of manufacturing a microstructure at atmospheric pressure and room temperature.

In the microstructure manufacturing apparatus according to the present invention, a mixture solution injection hole into which a mixture solution of distilled water and polyvinyl alcohol (PVA) is injected, a hollow part of a discharge pipe communicating with the mixture solution injection hole, and a spray hole is located at the front end of the hollow part, It is composed of a first injection nozzle to inject, the polymer solution injected from the first injection nozzle passes through the center of the mixed liquid passing through the hollow portion in a concentric circular flow is cured by ultraviolet irradiation means to produce a microwire in the laminar flow phenomenon It features.

In addition, in the present invention, a solution that is not mixed with water such as mineral oil is injected into the mixed solution injection hole so that the flow of the polymer solution is formed into a droplet shape, and the droplet-shaped polymer solution is cured by ultraviolet irradiation means. It is characterized in that the microbead (bead) is produced.

In addition, in the present invention, a polymer injection hole communicating with the first injection nozzle to inject the polymer solution, and a injection hole having a diameter smaller than that of the injection hole of the first injection nozzle are located in the center of the hollow portion of the first injection nozzle, The mixture further comprises a second injection nozzle to which a mixture of polyvinyl alcohol (PVA) is injected, such that the mixed solution injected from the second injection nozzle flows into the polymer solution injected from the first injection nozzle and passes through the hollow part. The polymer solution is allowed to flow out of the polymer solution, and the polymer solution is cured by ultraviolet irradiation means while passing through a tubular flow, thereby producing a microwire tube with laminar flow.

Therefore, according to the present invention, the device manufacturing cost is low, and it is possible to manufacture under normal temperature and atmospheric pressure without constraints such as high temperature and ultra high pressure, and very easy and economical microstructures can be manufactured. In addition, it is possible to manufacture structures of various diameters by controlling only the flow rate of the fluid, so that the commercial utility is very high.

Microwires, tubes, manufacturing equipment, manufacturing method, photocurable polymers, microfluidics

Description

Apparatus and method for manufacturing microstructures using microchannels {Apparatus and Methods for Manufacturing micro-structure}

1 is a cross-sectional perspective view of a microwire manufacturing apparatus according to an embodiment of the present invention.

Figure 2 is a photograph showing a microwire manufactured using a microwire manufacturing apparatus according to an embodiment of the present invention.

3 is a view showing a microbead manufacturing state using a microwire manufacturing apparatus according to an embodiment of the present invention.

Figure 4 is a photograph showing a microbead (microbead) manufactured by a microwire manufacturing apparatus according to an embodiment of the present invention.

5 is a graph showing the correlation between the injection speed of the injected polymer solution and the diameter of the microwire and the flow rate of the injected mixed solution.

6 is a flow chart showing a microwire manufacturing method according to an embodiment of the present invention.

Figure 7 is a cross-sectional perspective view of the microwire tube manufacturing apparatus according to the embodiment of the present invention.

Figure 8 is a photograph showing a microtube (microtube) manufactured by a microwire tube manufacturing apparatus according to an embodiment of the present invention.

9 is a flowchart illustrating a method for manufacturing a micro wire tube according to an embodiment of the present invention.

10 is a perspective view showing a microwire manufacturing apparatus partitioned into two layers.

** Description of the symbols for the main parts of the drawings **

100: micro wire manufacturing apparatus

200: micro tube manufacturing apparatus

300: compartmentalized micro wire manufacturing apparatus

1: main body 1a: mixed liquid injection hole

1b: polymer injection hole 2: first injection nozzle

2a: injection hole of the first injection nozzle 2b: hollow part of the first injection nozzle

3: discharge pipe 3a: hollow part of the discharge pipe

4: second injection nozzle 4a: injection hole of second injection nozzle

5: micro wire tube 6: UV irradiation means

7: microbead

The present invention relates to a microstructure manufacturing apparatus and a manufacturing method using the same, and more particularly, to a manufacturing apparatus and a manufacturing method using a microstructure, such as microwires, microtubes, microbeads.

In general, an extrusion method is used to manufacture microstructures such as microwires, microtubes, microbeads, and the like. Microwires, tubes, and micro beads (beads) manufactured in this manner are recently used in biotechnology, chemistry, and the like.

Conventional injection method is carried out under high temperature and ultra-high pressure to manufacture the microstructure. Therefore, the injection molding machine is made large in order to withstand this condition. The large sized injection molding machine takes a lot of manufacturing cost and is subject to space limitations. In addition, since it is manufactured under high temperature and ultra high pressure, there is a disadvantage in that it is not easy to combine various chemicals, enzymes and the like to the microwire.

In addition, there is a problem in that it takes a lot of cost and difficulty in manufacturing an injection mold because it is not a prefabricated one-piece.

On the other hand, in order to control the diameter of the microwire manufactured by the conventional injection method, it is necessary to control the injection pressure and temperature, etc., and replace with a suitable mechanism. Therefore, it is not easy to control this, and an additional device for controlling it is required, which results in a complicated device.

Accordingly, the present invention has been made to solve the problems of the prior art as described above, the object of the present invention is that it is possible to manufacture the device without the constraints of space, easy to manufacture, manufacturing cost and wire manufacturing of the device It is to provide a cost-effective microstructure manufacturing apparatus and a manufacturing method using the same. The ultimate object of the present invention is to implement a microstructure manufacturing apparatus that can be manufactured on a desk. It also provides a method for easily attaching biological and chemical substances such as enzymes, antigen-antibodies to the microstructures.

According to a feature of the present invention for achieving the object as described above, as shown in Figure 1, the mixed liquid injection hole (1a) is a mixture of distilled water and polyvinyl alcohol (PVA) is injected, and the mixed liquid injection hole ( It consists of a hollow portion (3a) of the discharge pipe (3) in communication with the 1a), and the first injection nozzle (2) for injecting the polymer solution, the injection hole (2a) is located at the front end of the hollow portion (3a), The polymer solution injected from the first injection nozzle 2 is cured by the ultraviolet irradiation means 6 while passing through the center of the mixed solution passing through the hollow portion 3a in a concentric manner, thereby producing a microwire in a laminar flow phenomenon. It is characterized by.

In addition, in the present invention, as shown in Figure 3, a solution that is not mixed with water such as mineral oil is injected into the mixed solution injection hole (1a) so that the flow of the polymer solution is formed in the shape of droplets (droplet), This droplet-shaped polymer solution is cured by ultraviolet irradiation means 6, characterized in that a microbead 7 is produced.

In addition, the present invention, as shown in Figure 7, the first injection nozzle (2) is in communication with the polymer injection hole (1b) for injecting a polymer solution, and the hollow portion of the first injection nozzle (2) (2b) a second injection nozzle (4a) having a diameter smaller than that of the injection hole (2a) of the first injection nozzle (2), the injection of a mixture of distilled water and polyvinyl alcohol (PVA) (2b) 4) further comprising a mixed liquid injected from the second injection nozzle 4 to flow into the polymer solution injected from the first injection nozzle 2 and passing through the hollow portion 3a. The polymer solution is allowed to flow out of the polymer solution, and the polymer solution passes through a tubular flow and is cured by the ultraviolet irradiation means 6 to produce a laminar flow microwire tube.

In the method of manufacturing a microstructure according to the present invention, a photocurable polymer solution spraying step 610 of injecting a photocurable polymer solution through the first injection nozzle 2 and distilled water to flow out of the sprayed photocurable polymer solution And a mixed solution injection step 620 for injecting a mixed solution of polyvinyl alcohol (PVA) and a polymer solution injected from the first injection nozzle 2 in a concentric manner in the center of the mixed solution injected from the mixed solution injection hole 1a. Ultraviolet curing step of curing the polymer solution flowing in the concentric phase discharged to the discharge pipe (3) to form a laminar flow while passing through the discharge pipe (3) using the ultraviolet irradiation means (6) It comprises 640, characterized in that the micro wire is manufactured.

In addition, the mixed solution injection step 620 is characterized in that the microbead (bead) is prepared by injecting a solution that is not mixed with water, such as mineral oil, so that the polymer solution flows into the droplet (droplet) shape.

In addition, before the discharge step (630), further comprises a mixed solution spraying step (600) for injecting a mixture of distilled water and polyvinyl alcohol (PVA) to the first injection nozzle (2), the photocurable polymer solution In the spraying step 610, the mixed solution is sprayed to flow into the polymer solution injected from the first injection nozzle 2, so that the polymer solution flows into an empty tube, and thus the microwire tube is manufactured.

In addition, the present invention, as shown in Figure 10, further comprises a separating plate (2c) located inside the first injection nozzle (2) divided into one or more partitioned space, the polymer solution with different components It is characterized in that the micro-wire having one or more components are integrally manufactured by injecting the separated into the partitioned space to be injected into the first injection nozzle (2).

Hereinafter, with reference to the accompanying drawings a preferred embodiment of a microstructure manufacturing apparatus and a manufacturing method using the same according to the present invention having the configuration as described above will be described in detail.

1 is a cross-sectional perspective view of a microwire manufacturing apparatus according to an embodiment of the present invention.

As shown, the micro-wire manufacturing apparatus 100 according to an embodiment of the present invention, the main body 1 is formed in communication with the mixed liquid injection hole (1a) is formed with a mixed liquid injection hole (1a) is injected, And a first injection nozzle 2 into which the photocurable polymer solution is injected and injected, a discharge pipe 3 through which the mixed solution and the injected polymer solution are discharged, and ultraviolet irradiation means 6 for curing the injected polymer solution.

The main body 1 has the mixed liquid injection hole (1a) is injected into the upper or lower portion of the mixed solution of distilled water and polyvinyl alcohol (PVA), the mixed liquid injection hole (1a) is a through the main body 1 and In communication.

The first injection nozzle 2 is inserted into the main body 1 and injected with a photocurable polymer solution. The first injection nozzle (2) is installed so that the injection hole (2a) is located in the center of the hollow portion (3a) of the discharge pipe (3) which is in communication with the mixed liquid injection hole (1a) through the injection hole (2a) The chemical polymer solution is injected and sprayed. At this time, the photocurable polymer solution used is a solution such as 4-hydroxybutyl acrylate (4-HYDROXYBUTYL ACRYLATE), and mixed with various chemicals, biological substances such as enzymes, antigen-antibodies, etc. Since the structure can be manufactured, it can be applied as a drug delivery medium or a micro sensor. It can also be used using chemical reactants in place of photocurable polymer solutions. When the photocurable polymer is flowed through the first injection nozzle 2, and a solution of PVA and distilled water is flowed into the mixture injection hole 1a, as shown in the circular diagram, the photocurable polymer is formed from the point A to the center. A concentric fluid flow consisting of a mixed solution (b) is formed outside the solution (a). Since the structure itself is a microsystem, this concentric fluid flow will continue for a considerable distance. Therefore, when ultraviolet rays are irradiated to the concentric fluid flow discharged to the discharge pipe, the photocurable polymer solution is rapidly cured to become a wire. This hardened wire is discharged without being stuck or entangled on the inner wall of the discharge pipe 3 while floating in the mixed liquid.

The discharge pipe (3) is located in front of the injection side of the first injection nozzle (2) and is inserted into the main body (1) to communicate with the mixture liquid injection hole (1a). Since the discharge pipe 3 discharges the mixed solution injected from the mixed solution injection hole 1a and the polymer solution injected from the first injection nozzle 2 while passing through the center of the hollow portion 3a, the mixed solution injection hole ( The mixed liquid injected from 1a) is discharged while contacting each other to the outside of the polymer solution injected from the first injection nozzle 2. Therefore, the mixed liquid discharged to the hollow portion 3a of the discharge pipe 3 acts on the surface of the injected polymer solution and is irradiated by the ultraviolet irradiation means 6 located on the discharge side of the discharge pipe 3. It prevents the tangling of the wire cured by the ultraviolet rays to serve as a smooth discharge to the discharge pipe (3).

The ultraviolet irradiation means 6 is located on the discharge side of the discharge pipe 3, and cures the polymer solution discharged from the discharge pipe (3). Therefore, when the ultraviolet ray (Ultraviolet Rays) is irradiated to the discharged polymer solution, the photocurable polymer is rapidly cured, and the cured polymer is attached to the inner wall of the discharge pipe 3 while being suspended in the mixed solution in the form of a wire. Will be discharged without 2 is a view showing a continuous state of the cured wire, the diameter of the wire is 1mm.

In the microwire manufacturing apparatus 100 having the above-described configuration, the mixed liquid injection hole 1a is formed, and the first injection nozzle 2 is inserted into the main body portion 1 in communication with the mixed liquid injection hole 1a. , The discharge pipe (3) is manufactured by being inserted to communicate with the mixed liquid injection hole (1a) from the discharge side. Therefore, the assembly is easily manufactured, the manufacturing cost of the device and the wire manufacturing cost is low, there is an advantage that can be manufactured on the desk without space constraints. In addition, there is an advantage that can be manufactured at room temperature and atmospheric pressure without restrictions such as high temperature and ultrahigh pressure.

3 is a view illustrating a state in which microbeads are manufactured. As shown in the drawing, when an immiscible solution such as mineral oil is used instead of a mixture of distilled water and polyvinyl alcohol (PVA) injected into the mixed liquid injection hole 1a, the first injection nozzle is used. In the portion A injected from the injection hole 2a of (2), the flow of the photocurable polymer solution is formed into a droplet shape by a phenomenon of minimizing the surface area, which is cured using ultraviolet (UV) light. This makes it possible to produce a microbead 7 as shown in FIG.

In addition, the diameter of the microwire can be varied by controlling the injection speed of the injected polymer solution and the flow rate of the mixed solution injected from the mixed solution injection hole 1a.

5 is a graph showing the correlation between the injection speed of the injected polymer solution and the diameter of the microwire and the flow rate of the injected mixed solution. As shown, as the sample flow rate of the polymer solution increases, the contact time with the mixture solution injected from the mixed solution injection hole 1a is short, so that the diameter of the injected polymer solution becomes large. As the flow rate of the mixed solution injected from the injection hole 1a increases, the diameter of the injected polymer solution decreases.

Therefore, by using the micro-wire manufacturing apparatus and the manufacturing method using the same according to the present invention, it is possible to manufacture a very easy and economical microstructure, and also to produce a microstructure of various diameters by controlling the flow rate of the fluid There is this.

6 is a flowchart illustrating a method for manufacturing a micro wire according to an embodiment of the present invention. With the above configuration, the microwires are manufactured by the following method using the microwire manufacturing apparatus 100.

Injecting the photocurable polymer solution through the first injection nozzle (2) (610), injecting a mixture of distilled water and polyvinyl alcohol (PVA) to flow to the outside of the injected photocurable polymer solution 620, the laminar flow is concentrically discharged into the discharge pipe 3 by the mixed solution injected from the mixed solution injection hole 1a to the outside of the polymer solution injected from the first injection nozzle 2 (630), The micro wire is manufactured by curing the polymer solution flowing in the concentric shape discharged to the discharge pipe 3 using the ultraviolet irradiation means 6 (640).

In addition, if a mixed solution of distilled water and polyvinyl alcohol (PVA) injected into the mixed liquid injection hole 1a is used, an immiscible solution such as mineral oil may be used. It becomes possible to manufacture.

Figure 7 is a cross-sectional perspective view of a micro wire tube manufacturing apparatus according to an embodiment of the present invention.

As shown, the micro-wire tube manufacturing apparatus 200 according to an embodiment of the present invention, the mixed liquid injection hole (1a) to be injected with the mixed liquid and the polymer injection hole (1b) into which the photocurable polymer solution is injected is formed The main body 1 through which the injection holes 1a and 1b communicate, the first injection nozzle 2 through which the photocurable polymer solution injected from the polymer injection hole 1b is injected, and the first injection nozzle 2 A second injection nozzle (4) for injecting a mixed solution so as to penetrate the inside of the photocurable polymer solution injected from the first injection nozzle (2), a mixed liquid penetrating the inside, and a tubular shape outside the mixed liquid A discharge pipe 3 through which the mixed solution flowing out of the polymer solution flowing into the tubular liquid and the polymer solution flowing into the tubular liquid is discharged, and ultraviolet ray irradiation means 6 for curing the polymer solution flowing into the injected tubular liquid.

The main body 1 has a mixture injection hole 1a into which a mixture of distilled water and polyvinyl alcohol (PVA) is injected and a polymer injection hole 1b into which a photocurable polymer solution is injected, and the injection holes 1a and 1b are formed. ) Is in communication with the main body 1.

The first injection nozzle 2 is inserted into the main body and the photocurable polymer solution injected into the polymer injection hole 1b is injected. The first injection nozzle (2) is installed so that the injection hole (2a) is located in the center of the hollow portion (3a) of the discharge pipe (3) which is in communication with the mixed liquid injection hole (1a) through the injection hole (2a) The chemical polymer solution is injected and sprayed. Therefore, as shown in the side view of the point B, the inside becomes a mixed liquid and the outside becomes a polymer solution to enter the first injection nozzle 2.

The second injection nozzle 4 is inserted into the main body 1 so as to be injected into the hollow portion 2b of the first injection nozzle 2 to inject a mixture of distilled water and polyvinyl alcohol (PVA). The diameter of the injection hole 4a of the second injection nozzle 4 is smaller than the diameter of the injection hole 2a of the first injection nozzle 2. Thus, since the diameter of the injection hole 4a of the said 2nd injection nozzle 4 is smaller than the diameter of the injection hole 2a of the said 1st injection nozzle 2, as shown in the side view of the point A, the said 1st The mixed liquid injected from the injection hole 4a of the second injection nozzle 4 is injected into the photocurable polymer solution a injected from the injection hole 2a of the injection nozzle 2, thereby mixing the mixture solution b into the polymer solution. ) Flows, and the mixed solution (c) injected into the mixed solution injection hole (1a) flows to the outside of the polymer solution in which the mixed solution flows to be discharged to the discharge pipe. That is, the inside and outside of the polymer solution injected at the point A becomes a tubular fluid flow in which the mixed solution flows. Again, because the structure itself is a microsystem, this tubular fluid flow will continue for a considerable distance. Accordingly, when ultraviolet rays are irradiated to the tubular fluid flow discharged to the hollow portion 3a of the discharge pipe 3, the photocurable polymer solution is rapidly cured, and the hollow microwire tube 5 as shown in FIG. )

Therefore, the micro wire tube 5 is manufactured by the method as shown in FIG. 9 using the micro wire tube manufacturing apparatus 200 having the above configuration.
In the manufacturing step of the micro-wire tube 5, the mixed solution of distilled water and polyvinyl alcohol (PVA) is injected into the second injection nozzle 4 so as to flow inside the polymer solution injected from the first injection nozzle 2. Spray (600).

The photocurable polymer solution is injected into the polymer injection hole 1b, and the photocurable polymer solution is injected through the first injection nozzle 2 (610), and distilled water and poly are flowed to the outside of the injected photocurable polymer solution. The mixed liquid of vinyl alcohol (PVA) is injected (620), and the polymer solution injected from the first injection nozzle (2) forms a laminar flow by the mixed liquid injected from the mixed liquid injection hole (1a), and the discharge pipe (3) It is discharged to the hollow portion 3a (630). Subsequently, the microwire tube 5 is manufactured by curing the polymer solution flowing in the tubular shape discharged to the hollow portion 3a of the discharge pipe 3 by using the ultraviolet irradiation means 6 (640). The microwire tube 5 produced in this way is used for vessels in the form of blood vessels or other tubes to move the same amount of material.

10 is a perspective view showing a micro wire manufacturing apparatus 300 partitioned into two layers.

As shown, the first injection nozzle 2 into which the polymer solution is injected and injected is divided into two compartments by the separating plate 2c, and the polymer solution is injected into the divided spaces by varying each component. By spraying with the injection nozzle 2, as shown in the side view of the point A, the polymer solution (a, b), which is divided in half, is injected, and the mixed solution (c) flows outside the micro-part divided into two. Wire 300 is manufactured. As such, the polymer solution having different components such as DNA, RNA, enzyme, antibody, and the like is separately injected and sprayed into each partitioned space so that the micro wires are integrally manufactured to efficiently perform various reaction tests simultaneously. do.

 As described above, according to the present invention, the manufacturing cost of the device is low, and it can be manufactured at room temperature and atmospheric pressure without restrictions such as high temperature and ultra high pressure, and very easy and economical microstructure can be manufactured. Due to these characteristics, it is possible to manufacture functional microstructures having chemical or biological substances, and because of the advantage of manufacturing structures of various diameters by controlling only the flow rate of the fluid, the commercial utility is very high.

Claims (7)

A mixed liquid injection hole 1a into which a mixed liquid of distilled water and polyvinyl alcohol (PVA) is injected, A hollow portion 3a of the discharge pipe 3 communicating with the mixed liquid injection hole 1a, The injection hole 2a is positioned at the front end of the hollow part 3a and is composed of a first injection nozzle 2 for injecting a polymer solution, and the polymer solution injected from the first injection nozzle 2 is formed in the hollow part. Microstructure manufacturing apparatus characterized in that the microwire is produced by laminar flow phenomenon by curing by ultraviolet irradiation means (6) while passing through the center of the mixed liquid passing through (3a) in a concentric flow. The method of claim 1, A solution that is not mixed with water such as mineral oil is injected into the mixed solution injection hole 1a to form a flow of the polymer solution in a droplet shape, and the droplet-shaped polymer solution is applied to the ultraviolet irradiation means 6. Microstructure manufacturing apparatus characterized in that the hardened by the manufacture of a microbead (bead). The method of claim 1, A polymer injection hole 1b communicating with the first injection nozzle 2 to inject a polymer solution, The injection hole 4a having a diameter smaller than that of the injection hole 2a of the first injection nozzle 2 is located at the center of the hollow part 2b of the first injection nozzle 2, and distilled water and polyvinyl alcohol (PVA) It further comprises a second injection nozzle (4) is injected to the mixture of the liquid, so that the mixed liquid injected from the second injection nozzle (4) flows into the polymer solution injected from the first injection nozzle (2) And the mixed solution passing through the hollow portion (3a) flows to the outside of the polymer solution, the polymer solution passes through the tubular flow and is cured by the ultraviolet irradiation means (6) to produce a laminar flow microwire tube (tube) Microstructure manufacturing apparatus characterized in that the. A photocurable polymer solution injection step 610 of injecting a photocurable polymer solution through the first injection nozzle 2; A mixed liquid injection step 620 of injecting a mixed liquid of distilled water and polyvinyl alcohol (PVA) to flow out of the sprayed photocurable polymer solution; Discharge step 630, the polymer solution injected from the first injection nozzle (2) is discharged to the discharge pipe (3) forming a laminar flow while passing through the center of the mixed solution injected from the mixed liquid injection hole (1a) in a concentric circular flow, And And a UV wire curing step (640) for curing the polymer solution flowing in the concentric circles discharged to the discharge pipe (3) using ultraviolet irradiation means (6). The method of claim 4, wherein The mixed solution injection step 620 is a microstructure manufacturing method characterized in that the microbeads are manufactured by injecting a solution that is not mixed with water such as mineral oil so that the polymer solution flows in the shape of droplets. The method of claim 4, wherein Before the photocurable polymer solution spraying step 610, and further comprises a mixed liquid spraying step 600 for spraying a mixture of distilled water and polyvinyl alcohol (PVA) to the second spray nozzle (4), the photocurable In the polymer solution spraying step 610, a micro-wire tube is manufactured by injecting a mixed solution to flow into the polymer solution injected from the first injection nozzle 2 so that the polymer solution flows into an empty tube. Microstructure manufacturing method. The method of claim 1, It further comprises a separating plate (2c) located in the first injection nozzle 2 divided into one or more partitioned space, by separating and injecting a polymer solution of different components into the partitioned space the first injection nozzle ( 2) The microstructure manufacturing apparatus, characterized in that the microwire divided into one or more are manufactured integrally by spraying.

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