KR101065614B1 - Micro-Pump for Lab-on-a-chip and the Method of producting that - Google Patents

Abstract

The present invention relates to a micropump for a lab-on-a-chip, a micropump manufacturing method, and a fluid transfer method using the same. In a micro-pump for a lab-on-a-chip, a temperature in at least a portion of a micro-channel of a lab-on-a-chip is Fixing the temperature-sensitive polymer shrinking or expanding in accordance with the change, by controlling the temperature of the microchannel portion in which the temperature-sensitive polymer is fixed to move the microfluid in the microchannel by the contraction or expansion of the temperature-sensitive polymer The micro-pump for a lab-on-a-chip, and according to the present invention, it is possible to move the fluid on the micro-channel with a simple configuration can contribute to simplify and miniaturization of the lab-on-a-chip.

Lab-on-a-chip, temperature sensitive, microchannel, micropump.

Description

Micro Pump for Lab-on-a-Chip and Micro Pump Manufacturing Method. {Micro-Pump for Lab-on-a-chip and the Method of producting that}

The present invention relates to a micropump for a lab-on-a-chip and a method for manufacturing a micropump, and more particularly, to fix a thermosensitive polymer on a micro-channel of a lab-on-a-chip and shrink or expand the temperature-sensitive polymer according to temperature control. The present invention relates to a micropump for moving a fluid on the microchannel and a method of manufacturing the same.

Biochip refers to the accumulation of biomolecules such as DNA and protein on a small substrate made of glass, silicon or nylon.In this case, the DNA is called DNA chip when the DNA is accumulated, and the protein chip when the protein is integrated This is called. Biochips can also be broadly divided into microarray chips and microfluidics chips.

Microarray chips are biochips that can arrange thousands or tens of thousands or more of DNA or proteins at regular intervals, process analytes, and analyze their binding patterns. Microfluidics chip is a biochip that analyzes the reaction of various kinds of biomolecules or sensors integrated in the chip while flowing a small amount of analyte. It is also called a lab on a chip. It is a cutting-edge technology that combines the functions of sensor, pump, valve, reactor, extractor, separation system, etc., which are essential for the sample pretreatment process of the automatic analyzer used for the analysis of biochemicals.

Looking more closely at Lab-on-a-Chip, Lab-on-A-Chip is designed to perform sample injection, pretreatment, chemical reactions, separation / analysis, etc., which are performed at the laboratory level in order to analyze chemical and biochemical materials. It is a microanalysis device.

Lab-on-a-chip technology combines micro flow control technology with MEMS micromachining technology to accurately transfer, distribute and mix samples from several picoliters (pl) to tens of microliters (μl). It is a core technology.

Lab-on-a-Chip, which uses trace amounts of samples and analyzes chemical components quickly and easily, is widely used to select useful new drugs out of many new drug candidates at high speed.In recent years, lab-on-a-chip is used to detect environmental pollutants and diagnose diseases. Kind of lab-on-a-chip is under research and development.

Unlike micro-array chips such as DNA chips and protein chips, lab-on-a-chips are still at the stage of R & D in the world, and commercialization is limited and small-scale. In the case of a lab-on-a-chip, the network of microchannels is simple and the reaction process is implemented at a stage not complicated.

In order for Lab-on-A-Chip to play a full role in the BT industry, it must have a function as a micro-to-analysis system (micro-TAS) that can accurately and quickly perform the complex test procedures in hospitals. Therefore, it is necessary to develop a new method of microfluidic control system that can effectively control microfluidic in a complex microchannel network.

Currently, the system for controlling the micro-flow inside the lab-on-a-chip uses a simple pressure load or an electric drive method. A small pump connected or mechanical pressure was applied to flow the fluid.

1 illustrates a method of manipulating a fluid in a substrate, such as a wrap-on-a-chip in accordance with the prior art.

As a prior art, a method of manipulating fluid in a substrate (Patent No.:10-0451154) is a mechanical pressure for directly contacting a microchannel in a substrate made of elastic polymer with the substrate directly from the outside as shown in FIG. In order to prevent the flow of fluid, the externally applied portion is moved in the longitudinal direction of the microchannel to push the fluid contained in the microchannel in a desired direction as shown in FIG. 1A or as shown in FIG. Pull in direction to feed.

However, according to the related art, a separate driving device is required to move the fluid in the microchannel, and the configuration of the biochip such as the lab-on-a-chip is complicated.

Furthermore, according to the technology development, the biochip is bound to the technology of NT field. Therefore, the size of the biochip is inevitably smaller. However, if the conventional technology requires a separate driving device, the size of the biochip is limited. It is an obstacle to simplification and miniaturization.

The present invention is intended to solve the problem that the configuration of a biochip such as a lab-on-a-chip according to the installation of a separate drive to move the fluid in the micro-channel on the lab-on-a-chip.

Furthermore, it is an object of the present invention to provide a micro-pump for a lab-on-a-chip that can contribute to the miniaturization of the lab-on-a-chip without the need for additional external machinery.

In order to solve the above problems, the present invention, in the lab-on-chip micro-pump, fixing a temperature-sensitive polymer that shrinks or expands in accordance with a temperature change to at least a portion of the inside of the micro-channel of the lab-on-a-chip Let's

The micro-pump for a lab-on-a-chip is characterized in that the microfluidic fluid in the microchannel is moved by contracting or expanding the temperature-sensitive polymer by controlling the temperature of the microchannel in which the temperature-sensitive polymer is fixed.

Preferably, the micro-channel, the capillary tube is formed on one side is formed with an inlet port for the introduction of the microfluid and the other side is discharge port for the discharge of the microfluid; A temperature sensitive polymer fixed along the capillary inner surface; And a temperature adjusting means positioned on the outer surface of the capillary tube or on the microchannel in contact with the capillary tube to apply heat to the temperature sensitive polymer.

Furthermore, the microchannel may be divided into a plurality of zones, and the temperature adjusting means may include a plurality of microelectrodes positioned in each of the plurality of zones and a controller for controlling a current supply to the plurality of microelectrodes.

Preferably, the temperature control means, the first micro-electrode positioned near the inlet of the micro-channel on the wrap-on-a-chip substrate; A second microelectrode positioned near an outlet of the microchannel on the wrap-on-a-chip substrate; And a third microelectrode positioned between the first microelectrode and the second microelectrode.

Herein, a polymer hydrogel may be applied as the temperature sensitive polymer, and more preferably, poly N isopropyl acrylamide (PNIPAAm) may be applied as the temperature sensitive polymer.

The present invention also provides a method for manufacturing a micro pump for a lab on a chip, comprising: a) injecting a thermosensitive polymer and a crosslinking agent for fixing the thermosensitive polymer onto a capillary tube, and then Heating to a temperature to fix the temperature sensitive polymer to the capillary inner surface; And b) forming a temperature control means for applying heat to the capillary on the wrap-on-a-chip substrate on which the microchannel is to be placed, and mounting the capillary to which the temperature sensitive polymer is fixed to the microchannel. It is a manufacturing method of a microchip for on-chip.

Preferably, the step a) comprises the steps of: a-1) injecting a surfactant into the capillary for treating the inner surface of the capillary; a-2) injecting a temperature sensitive polymer and a crosslinking agent to fix the temperature sensitive polymer to the inner surface of the capillary tube into the inner surface treated capillary tube; And a-3) heating the capillary tube to a predetermined temperature to fix the temperature sensitive polymer to an inner surface of the capillary tube.

Furthermore, in the step a-2), a catalyst for promoting the fixing of the temperature sensitive polymer may be further injected into the inner surface of the capillary.

Here, as the temperature sensitive polymer, poly N isopropyl acrylamide (PNIPAAm) may be used.

In addition, as the surfactant in step a-2), 3-propyl methacrylic acid (3- (trimethoxysilyl) propyl methacrylate) may be used, and as a crosslinking agent in step a-2), N, N ' -Methylenebisacrylamide (N, N'-methylenebisacrylamide) may be used, and as the catalyst, 2,2'-azobis (2,2'-azobis (isobutyronitrille)) may be used.

Furthermore, the step b) may include b-1) dividing the microchannel into a plurality of zones in a longitudinal direction and forming temperature control means in each of the plurality of zones; And b-2) mounting a capillary tube on which the temperature sensitive polymer is fixed on the microchannel on which the temperature adjusting means is formed.

Preferably, the step b-1) may include forming a microelectrode at each position corresponding to a plurality of zones of the microchannel on the substrate of the lab-on-a-chip on which the microchannel is to be formed.

More preferably, the step b-1) may include a first microelectrode near the inlet of the microchannel on the substrate of the lab-on-a-chip and a second microelectrode near the outlet of the microchannel. And forming a third microelectrode between the first microelectrode and the second microelectrode.

According to the present invention, it is possible to simplify the configuration of a lab-on-a-chip by providing a micropump capable of moving a fluid on a microchannel without using a bulky mechanical device, and at the same time, contributing to the miniaturization of the lab-on-a-chip. Will be.

Furthermore, it is possible to provide a micro pump for a lab-on-a-chip that can effectively control the microfluid through a simple manufacturing process, thereby lowering the manufacturing cost of the lab-on-a-chip.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects, features and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings.

2 shows an embodiment in which a micro pump according to the present invention is mounted on a micro channel of a lab-on-a-chip.

The lab-on-a-chip 100 injects a sample into an inlet and injects a reagent on the reagent chamber 400 so that the sample and the reagent are moved to the reaction chamber through a micro channel, and the sample and the reagent react on the reaction chamber. It will be discharged to the outlet.

The lab-on-a-chip 100 should effectively control the movement of microfluidics in a complex microchannel network. The present invention provides a micro pump for controlling the movement of microfluidics such as a sample or a reagent, and FIG. The embodiment is equipped with a micro-pump 200 according to the present invention for controlling the.

In the present invention, the temperature-sensitive polymer is fixed to at least a portion of the inside of the micro-channel of the lab-on-a-chip 100 to contract or expand according to temperature change, and the temperature-sensitive polymer is controlled by adjusting the temperature of the micro-channel portion to which the temperature-sensitive polymer is fixed. Contraction or expansion of the polymer causes the microfluidic fluid in the microchannels to move. Here, more preferably, the microchannel may be formed using a capillary tube and the temperature sensitive polymer may be fixed to the inner surface of the capillary tube.

As the thermosensitive polymer, a gel-type polymer hydrogel (Hydrogel) may be used, and poly N isopropyl acrylamide (PNIPAAm) may be used. The poly N isopropyl acrylamide (PNIPAAm) is usually suitable for use as a temperature sensitive polymer according to the present invention because it has a property of shrinking at 40 degrees or more and expanding at room temperature.

3 shows a perspective view of an embodiment of a micropump using a capillary tube according to the invention.

The microchannel on the lab-on-a-chip can be formed into a capillary tube. In the present invention, the micropump 200 can be mounted on the microchannel formed by the capillary tube, and FIG. 3 shows a temperature-sensitive polymer in the capillary tube 210. 250 represents a fixed embodiment.

As shown in FIG. 3, the microchannel of the lab-on-a-chip 100 has a temperature along an inner surface of a capillary tube 210 on which one side is formed with an inlet through which microfluid is introduced, and the other side has a discharge port through which the microfluid is discharged. The micro pump 200 for moving the microfluid in the capillary tube 210 may be mounted as the thermosensitive polymer 250 is contracted or expanded by adjusting the temperature of the thermosensitive polymer 250 by forming the sensitive polymer 250. have.

Figure 4 shows an embodiment of a temperature control means for controlling the temperature of the capillary 210, the temperature-sensitive polymer 250 is fixed as shown in FIG.

As shown in FIG. 4, a fine microelectrode is formed to control the temperature of the temperature sensitive polymer 250 and the current flow of the microelectrode is controlled to generate a temperature of the temperature sensitive polymer 250 with heat generated from the microelectrode. I can regulate it.

In the embodiment of FIG. 4, the microchannels on the lab-on-a-chip 100 are divided into a plurality of zones, and a portion 130 in which the microchannels on the substrate 101 of the lab-on-a-chip 100 is located is located in each of the plurality of zones. The plurality of microelectrodes 261, 262, 263, 264, 265, and 266 are formed to correspond to each other, and the controller 290a controls the current flow of the plurality of microelectrodes 261, 262, 263, 264, 265, and 266 to be fixed to the capillary tube 210 by heat generation from the plurality of microelectrodes 261, 262, 263, 264, 265, and 266. The temperature of the temperature sensitive polymer 250 can be adjusted.

The controller 290a may be located outside the wrap-on-a-chip 100 to supply current to control heat generation in the plurality of microelectrodes 261, 262, 263, 264, 265, and 266.

Figures 5a to 5c is an embodiment showing a cross section of the micro-channel for showing the operating principle of the micropump according to the present invention.

In FIG. 5A, the temperature sensitive polymer 250 is fixed along the inner surface of the capillary tube 210. The microchannel using the capillary tube 210 is divided into a plurality of sections, and microelectrodes 261 to 266 are formed in each section. It is.

When poly N isopropyl acrylamide (PNIPAAm) is used as the temperature sensitive polymer 250, FIG. 5A shows that all of the plurality of microelectrodes 261 to 266 generate heat and the temperature sensitive polymer 250 is contracted. It is a state.

As shown in FIG. 2B, when the temperature of the microchannel portion corresponding to the first microelectrode 261a is lowered by blocking the current flowing through the first microelectrode 261a, poly N isopropyl acrylamide is a temperature sensitive polymer 250. (PNIPAAm) expands to block the passage on the microchannel.

FIG. 2C shows the same process as in FIG. 2B, and when the current is blocked to the second microelectrode 262a, the temperature of the portion is lowered, so that poly N isopropyl acrylamide (PNIPAAm), which is a temperature sensitive polymer 250, is Since it expands, it is blocked up to the passage on the microchannel corresponding to the second microelectrode 262a.

Figure 6 shows another embodiment of the temperature control means for controlling the temperature of the capillary 210, the temperature sensitive polymer 250 is fixed according to the present invention.

In FIG. 6, the temperature adjusting means forms a first microelectrode 271 near the inlet of the portion 130 on which the microchannel on the lab-on-a-chip 100 substrate 101 is to be positioned, and the microchannel is positioned ( The second microelectrode 273 is formed near the outlet of the 130, and the third microelectrode 272 is formed between the first microelectrode 271 and the second microelectrode 273, and the first microelectrode is formed. (271), the control unit 290b for controlling the second microelectrode 273 and the third microelectrode 272 is formed.

7 shows a cross-sectional view of the embodiment of FIG. 6.

Since FIG. 7 is the same process as the embodiment of FIGS. 5A to 5C, a detailed description thereof will be omitted. Here, the first microelectrode 271 may block the inlet of the microchannel and simultaneously push the fluid on the microchannel. The three microelectrodes 272 actually push the fluid on the microchannel, and the second microelectrode 273 blocks the outlet of the microchannel while finally pushing the remaining fluid on the microchannel.

Then, the process of moving the fluid using a micro-pump for lab-on-a-chip according to the present invention will be described.

FIG. 8 illustrates a process of moving a fluid in the embodiment of FIGS. 4 to 5.

In FIG. 8A, heat is generated according to the operation of all the microelectrodes 261-266 of the micropump 200 to shrink the temperature sensitive polymer 250 to open the passage of the microchannel. The fluid 285 is introduced into the inlet of the capillary 210 on the microchannel to fill the fluid 285 on the microchannel.

As shown in FIG. 8B, the temperature sensitive polymer 250 fixed to the capillary 210 on the microchannel corresponding to the first microelectrode 261a is expanded by blocking the current flow of the first microelectrode 261a. As the passage portion on the microchannel corresponding to the first microelectrode 261a is blocked, the fluid located in this portion is pushed out in the right direction.

As shown in FIG. 8C, the temperature sensitive polymer 250 fixed to the capillary 210 on the microchannel corresponding to the second microelectrode 262a is expanded by blocking the current flow of the second microelectrode 262a. While pushing the fluid located in this area to the right side more.

8 (d) to (e) likewise control the current flow of each microelectrode 263a, 264a, and 265a so that heat generation of each microelectrode 263a, 264a, and 265a is cut off to correspond to a corresponding position. As the temperature sensitive polymer 250 expands, it continuously pushes the fluid present at each position to the right.

Eventually, when reaching (f) of FIG. 8, the current flowing through all of the microelectrodes 261a to 266a is cut off, and the temperature on the corresponding capillary 250 is lowered so that the temperature-sensitive polymer 250 expands. All the fluid filled on the microchannel on the N-axis moves in the direction of the right arrow.

9 illustrates a process of moving a fluid in the embodiment of FIGS. 6 to 7.

In FIG. 9A, similar to FIG. 8A, heat is generated in accordance with the operation of all the microelectrodes 271, 272, and 273 of the micropump 200 to shrink the temperature sensitive polymer 250 to allow passage of the microchannel. To open. The fluid 285 is introduced into the inlet of the capillary 210 on the microchannel to fill the fluid 285 on the microchannel.

In FIG. 9B, the temperature sensitive polymer 250 fixed to the capillary 210 on the microchannel corresponding to the first microelectrode 271a is expanded by blocking the current flow of the first microelectrode 271a. As the passage portion on the microchannel corresponding to the first microelectrode 271a is blocked, the fluid located at the portion is pushed out in the right direction. Here, as illustrated in FIG. 9B, the inlet of the microchannel may be blocked through the first microelectrode 271 and the fluid on the microchannel may be pushed to the right.

Thereafter, as shown in FIG. 9C, the temperature of the temperature sensitive polymer 250 on the capillary tube 210 corresponding to the third microelectrode 272a is lowered by cutting off the current of the third microelectrode 272a. The thermosensitive polymer in the part expands gradually, which results in a narrower passageway on the microchannel in this part, pushing the volume of fluid to the right.

Furthermore, as shown in FIG. 9D, when the temperature sensitive polymer 250 of the capillary tube 210 corresponding to the third microelectrode 272a is at room temperature, the passage of the microchannel of this part is completely blocked. As a result, the fluid is pushed further to the right.

As a result, as shown in FIG. 9E, when the current of the second microelectrode 273a is also blocked, the temperature sensitive polymer 250 of the capillary 210 corresponding to the second microelectrode 273a also expands, thereby allowing passage of the microchannel. Is completely blocked and any remaining fluid will be discharged to the right.

As described above, the micro-pump for the lab-on-a-chip according to the present invention can move the fluid on the microchannel without using a relatively bulky mechanical device, thereby simplifying the configuration of the lab-on-a-chip. It can also contribute to miniaturization.

The present invention provides a method for manufacturing a micro pump for a lab-on-a-chip, which will be described below.

10 is a schematic flowchart of a manufacturing method of a micro pump for a lab-on-a-chip according to the present invention.

In the present invention, the microchannel may be formed using a capillary tube. The method of manufacturing a micro-pump for a lab-on-a-chip according to the present invention is roughly injected by injecting a thermosensitive polymer and a crosslinking agent to fix the thermosensitive polymer onto the capillary tube. And heating the capillary tube to a predetermined temperature to fix the temperature sensitive polymer to the capillary inner surface, and forming temperature control means for applying heat to the capillary tube on the wrap-on-a-chip substrate where the microchannel is to be placed. It consists of the step of mounting the capillary fixed to the temperature sensitive polymer.

Looking at the process of fixing the temperature-sensitive polymer to the capillary tube in more detail with reference to Figure 11, to form a capillary 210 to be used as a micro channel of the lab-on-a-chip, the capillary 210 by elongating a silica tube, such as a fine channel It is possible to form a capillary with.

In order to effectively fix the temperature-sensitive polymer 250 inside the capillary tube 210, the inner surface of the capillary tube 210 is treated, as shown in FIG. The surfactant is injected (S10) and the inner surface is treated (S20) to facilitate fixing of the temperature sensitive polymer by the surfactant. In this case, 3-propyl methacrylic acid (3- (trimethoxysilyl) propyl methacrylate) may be used. FIG. 11 (c) shows the inner surface 220 of the capillary tube 210 treated with the surfactant.

When the inner surface of the capillary tube 210 is completed, as shown in (d) of FIG. 11, a thermosensitive polymer 250 and a crosslinking agent are injected into the capillary 210 (S30), wherein the thermosensitive polymer is a polymer hydrogel (Hydrogel). ), Poly N isopropyl acrylamide (PNIPAAm) may be used, and N, N'-methylenebisacrylamide may be used as a crosslinking agent.

Furthermore, a catalyst for promoting the fixing of the temperature sensitive polymer may be additionally injected such that the temperature sensitive polymer may be fixed in a faster time, and as the catalyst, 2,2'-azobis (2,2'-azobis) may be added. (isobutyronitrille)) can be used.

11 (e) shows a state in which a temperature sensitive polymer, a crosslinking agent, and a catalyst are injected into the capillary 210.

As such, after injecting the temperature sensitive polymer, the crosslinking agent, and the catalyst into the capillary tube and applying the heat to the capillary tube at about 40 ° C., the temperature of the capillary tube The temperature sensitive polymer is fixed (S40) along the inner surface, and FIG. 11 (f) shows a state in which the temperature sensitive polymer 250 is fixed by applying heat to the capillary 210.

Looking at the process of forming a temperature control device for controlling the temperature of the temperature-sensitive polymer 250, forming a temperature control means on the substrate of the lab-on-a-chip to place the micro channel (S50), wherein the micro channel It may be divided into a plurality of zones in the longitudinal direction and the temperature control means to correspond to each of the plurality of zones.

Preferably, a micro electrode may be formed at each position corresponding to the plurality of zones of the micro channel on the substrate of the wrap-on-a-chip on which the micro channel is to be formed, and a temperature adjusting means including the micro electrode may be formed. Preferably, on the substrate of the wrap-on-a-chip, a first microelectrode near the inlet of the microchannel, a second microelectrode near the outlet of the microchannel, and the first microelectrode and the second microelectrode A third microelectrode may be formed between the electrodes to be used as the temperature control means.

When the temperature control means is formed as described above, the microcapacitor for lab-on-a-chip according to the present invention is formed by mounting a capillary in which the temperature-sensitive polymer is fixed on the substrate of the lab-on-a-chip.

Here, although the temperature control means has been described as a process after the manufacture of the capillary tube 210 to which the temperature sensitive polymer 250 is fixed, this is only a process for convenience of description and after the formation of the temperature control means temperature sensitive polymer The capillary 210 to which the 250 is fixed may be manufactured and mounted on the lab-on-a-chip.

In this simple process, a micro-pump for a lab-on-a-chip can be manufactured, and since no additional mechanical device is required, the configuration is simple and the manufacturing cost can be lowered, thereby maximizing efficiency in manufacturing the lab-on-a-chip.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Accordingly, the embodiments described in the present invention are not intended to limit the technical spirit of the present invention but to describe the technical spirit of the present invention. The protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

1 illustrates a method of manipulating a fluid in a substrate such as a lab-on-a-chip according to the prior art,

2 shows an embodiment in which a micro pump according to the present invention is mounted on a micro channel of a lab-on-a-chip.

Figure 3 shows a perspective view of an embodiment of a micro pump using a capillary tube according to the present invention,

Figure 4 shows an embodiment of a temperature control means for controlling the temperature of the capillary tube is fixed to the temperature-sensitive polymer as shown in Figure 3,

5a to 5c is an embodiment showing a cross section of the micro-channel for showing the operating principle of the micro-pump according to the present invention,

Figure 6 shows another embodiment of the temperature control means for controlling the temperature of the capillary in which the temperature sensitive polymer is fixed according to the present invention,

7 shows a sectional view of the embodiment of FIG. 6,

8 illustrates a process of moving a fluid in the embodiment of FIGS. 4 to 5;

9 illustrates a process of moving a fluid in the embodiment of FIGS. 6 to 7;

10 is a schematic flowchart of a manufacturing method of a micro pump for a lab-on-a-chip according to the present invention;

11 illustrates a process of fixing a temperature sensitive polymer to a capillary tube to be used as a micro channel of a lab-on-a-chip according to the present invention.

<Description of Major Symbols in Drawing>

100: lab-on-chip, 200: micro pump,

210: capillary, 250: temperature sensitive polymer,

260 ~ 273: micro electrode,

290, 290, 290a, 290b, 295: passageway on the micro channel,

290a, 290b: micro electrode control unit.

Claims (16)

In the micro pump for Lab on a chip, A temperature-sensitive polymer that contracts or expands with temperature conversion is fixed along the inner circumference of the capillary tube formed inside the micro channel of the lab-on-a-chip. The micro channel is divided into a plurality of zones, each of the plurality of zones of the micro channel is provided with individual temperature control means, And the temperature control means controls the internal temperature of the microchannel to move the microfluid in the microchannel for each zone of the microchannel by contraction or expansion of the temperature sensitive polymer. The method of claim 1, The micro channel is, A capillary tube formed with an inlet through which one side of the microfluid flows and an outlet through which the microfluid is discharged; A temperature sensitive polymer fixed along the capillary inner surface; And And a temperature adjusting means positioned on the outer surface of the capillary tube or on the microchannel in contact with the capillary tube to apply heat to the temperature sensitive polymer. The method of claim 2, The micro channel is divided into a plurality of zones, The temperature control means is a micro-pump for a lab-on-a-chip, characterized in that it comprises a plurality of micro-electrodes positioned in each of the plurality of zones and a control unit for controlling the supply of current to the plurality of micro-electrodes. The method of claim 3, wherein The temperature control means, A first microelectrode positioned near an inlet of the microchannel on the lab-on-a-chip substrate; A second microelectrode positioned near an outlet of the microchannel on the wrap-on-a-chip substrate; And And a third microelectrode positioned between the first microelectrode and the second microelectrode. The method according to any one of claims 1 to 4, The temperature-sensitive polymer is a lab-on-a-chip micro pump, characterized in that the polymer hydrogel (Hydrogel). The method of claim 5, The temperature sensitive polymer is poly N isopropyl acrylamide (PNIPAAm), the micro-pump for lab-on-a-chip. In the manufacturing method of a micro pump for a lab on a chip, a) injecting a temperature sensitive polymer and a crosslinking agent for fixing the temperature sensitive polymer onto a capillary tube and then heating the capillary tube to a predetermined temperature to fix the temperature sensitive polymer along the circumference of the capillary inner surface; And b) forming a temperature control means for applying heat to the capillary on a wrap-on-a-chip substrate on which the microchannel is to be placed, and mounting the capillary to which the temperature-sensitive polymer is fixed to the microchannel. Method for producing a micro pump for a chip. The method of claim 7, wherein Step a) is a-1) injecting a surfactant into the capillary for treating the inner surface of the capillary; a-2) injecting a temperature sensitive polymer and a crosslinking agent to fix the temperature sensitive polymer to the inner surface of the capillary tube into the inner surface treated capillary tube; And a-3) The method of manufacturing a micro-pump for a lab-on-a-chip comprising heating the capillary to a predetermined temperature to fix the temperature sensitive polymer to the inner surface of the capillary. The method of claim 8, Step a-2), The method of manufacturing a micro-pump for a lab-on-a-chip, characterized in that further injection of a catalyst for promoting the fixing of the temperature-sensitive polymer on the inner surface of the capillary. The method of claim 8, The temperature sensitive polymer is poly N isopropyl acrylamide (PNIPAAm), the method for producing a micro-pump for a lab-on-a-chip. The method of claim 8, The surfactant in step a-2) is 3-propyl methacrylic acid (3- (trimethoxysilyl) propyl methacrylate) The manufacturing method of the micro-pump for lab-on-a-chip. The method of claim 8, The cross-linking agent of step a-2) is N, N'-methylenebisacrylamide (N, N'-methylenebisacrylamide) The manufacturing method of the micro-pump for lab-on-a-chip. The method of claim 9, The catalyst is a 2,2'-azobis (2,2'-azobis (isobutyronitrille)) method for producing a micro-pump for a lab-on-a-chip, characterized in that. The method of claim 7, wherein B), b-1) dividing the microchannel into a plurality of zones in a longitudinal direction and forming temperature control means in each of the plurality of zones; And b-2) a method for manufacturing a micro-pump for a lab-on-a-chip comprising mounting a capillary on which the temperature sensitive polymer is fixed on a micro channel on which the temperature adjusting means is formed. The method of claim 14, Step b-1), And forming a microelectrode at each position corresponding to a plurality of zones of the microchannel on the substrate of the wrap-on-a-chip on which the micro-channel is to be formed. The method of claim 14, Step b-1), On the substrate of the lab-on-a-chip, a first microelectrode near the inlet of the microchannel, a second microelectrode near the outlet of the microchannel, and between the first microelectrode and the second microelectrode The method of manufacturing a micro-pump for a lab-on-a-chip comprising the step of forming a third microelectrode.

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