KR100553265B1 - Composition for wet tissue comprising electrolyte and plant extract and wet tissue containing same - Google Patents

Abstract

 The present invention relates to a water tissue composition comprising an electrolytic water and forest resources extracts and water tissue containing the same. In particular, the present invention can significantly increase the antimicrobial effect by using electrolyzed water instead of purified water in water tissues, electrolytic water that can significantly suppress the growth of pathogenic food poisoning microorganisms and fecal contaminants by using forest resource extracts instead of conventional chemicals It provides a composition for water tissues comprising oak extract and rhubarb extract and water tissues containing the same.

Electrolyzed water, forest resource extract, water tissue, fecal contaminant bacteria, pathogenic food poisoning microorganism

Description

Composition for water tissue containing electrolyzed water and forest resources extract and water tissue containing same TECHNICAL FIELD

1 shows the degree of detection of total microorganisms over time in water tissues according to Examples 12 to 21.

Figure 2 shows the degree of detection of yeast and mold over time in water tissues according to Examples 12 to 21.

Figure 3 confirms the antimicrobial effect against the Listeria bacteria pathogenic food poisoning microorganisms in the water tissue of the present invention.

[TECHNICAL FIELD OF THE INVENTION]

The present invention relates to an electrolytic water and forest resource extract composition for water tissue and water tissue containing the same, and more specifically, electrolytic water, oak extract and rhubarb extract which can significantly inhibit the growth of pathogenic food poisoning microorganisms, fecal contaminant bacteria It relates to a composition for water tissue and water tissue comprising a.

[Private Technology]

Human life is enriched by various industrial, economic and cultural developments, but on the other hand, the incidence of various diseases such as adult diseases is increasing, as well as environmental pollution and bacterial contamination. Increasingly, interest in health is increasing.

In particular, from the point of view of food hygiene, researches to prevent the risks caused by decayed bacteria and pathogenic food poisoning microorganisms during distribution and preservation are becoming more important, and research fields to prevent infections or invasion of diseases with increased resistance in advance. Also pays much attention.

Wet tissue is a product that is sold for washing hands before meals, which are commonly used in restaurants. In addition, water tissues are produced in various forms for skin cleanliness.

Conventional water tissues are sterilized by adding purified water to a base for water tissues, and usually include surfactants, chemical preservatives, fragrances and alcohols. However, the use of chemicals can cause problems with skin stability, such as skin allergies, and can cause skin damage due to its strong cleansing action. Or there was a lack in removing pathogenic microorganisms.

In order to improve this, Korean Laid-Open Patent Publication No. 97-25529 discloses disposable water tissues containing natural active plant extracts such as thyme, marigold, Angelica, licorice, thought, peony, basswood, aloe, rosemary, garlic and oats. Korean Patent Laid-Open Publication No. 97-5276 discloses herbal bamboo salt water tissues impregnated with the addition of bamboo salt and grapefruit extract to a herbal medicine mixture. In addition, the Republic of Korea Patent Publication No. 2003-178452 discloses a water tissue containing germanium and eoseongcho, and the Republic of Korea Patent Publication No. 2003-8556 provides a water tissue composition for protecting the skin from dryness due to atopic dermatitis Suggesting.

It is an object of the present invention to provide a composition for antibacterial water tissues having increased antibacterial activity, including electrolyzed water, and water tissues containing the same.

In addition, an object of the present invention to provide a composition for antimicrobial water tissue comprising an electrolytic water and forest resources extract and water tissue containing the same.

It is another object of the present invention to provide a composition for antimicrobial water tissue and a water tissue containing the same.

In order to achieve the above object, the present invention provides a composition for water tissue comprising electrolyzed water, oak extract and rhubarb extract.

The present invention also provides a water tissue prepared by containing a composition for water tissue in a base paper.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for water tissue using electrolytic water and a water tissue comprising the same.

Electrolyzed water is water obtained by electrolysis. There are weakly electrolyzed and strongly electrolyzed water. The weak electrolytic water is a weak electrolytic reduced water and a weak electrolytic oxidation water prepared by passing a weak current through the installation of a separator that allows only ions to penetrate between the anode and the cathode while purifying impurities. The strong electrolytic water has 99% purity in water. Sodium chloride or potassium chloride is a strong electrolytic oxidation water and a strong electrolytic reduction water obtained when electrolysis is performed by flowing a strong current. Electrolytic reduced water is called reduced water, alkaline water or alkaline ionized water, and electrolytic oxidation water is called oxidized water, acidic water or acidic ionized water.

The electrolyzed water of the present invention may be weakly electrolyzed or strongly electrolyzed water, and acidic and alkaline water may be prepared by a conventional electrolytic water production method. Acidic water is charged with negative (-1) ions using a special ion diaphragm that can pass only ions with electrical properties. ^­ , Cl ^­ Etc.), as the electrons (e) are lost to the electrodes, chlorine gas (Cl ₂ ) and hypochlorous acid (HClO) are generated, and H ⁺ is increased and OH ⁻ is reduced. The physical properties of acidic water are pH 2.5 to 2.7 and redox potential (ORP) 600 to 1200 mV, with a preferred dissolved oxygen level of 25 ppm. Alkaline water is produced by using a special ion diaphragm that can pass only ions with electrical properties, so that positively charged ions (CH ⁺ ) receive electrons from the electrode, increase OH ⁻ , and decrease H ⁺ . . At this time, hydrogen gas (H ₂ ) is generated, a part of which is dissolved in water to produce a lot of dissolved hydrogen water. The physical properties of alkaline water are pH 11.0 to 11.5 and redox potential (ORP) -850 to -50 mV. The preferred dissolved oxygen content is 0 ppm and the dissolved hydrogen content is 400 to 500 ppb.

Electrolyzed water is used in water tissues to increase the antibacterial effect.

The water tissue of this invention contains the composition for water tissues in the base paper.

Base paper is a substrate commonly used in water tissue, for example, non-woven, woven, paper or pulp.

The composition for water tissue includes electrolyzed water and forest resource extracts. The forest extract is preferably an oak extract and / or a yellow lotus extract. The composition for the water tissue is 0.1 to 20% by weight of oak extract, 0.1 to 20% by weight of rhubarb extract and the balance of electrolyzed water. If the content of the oak extract and rhubarb extract is less than 0.1% by weight antimicrobial effect may be insignificant, if it exceeds 20% by weight may be uneconomical compared to the effect. Preferably the content of each oak extract and rhubarb extract is 0.5 to 2% by weight.

Each of the oak extract and the rhubarb extract is a dried product extracted with distilled water or an organic solvent as a solvent. Preferably, the oak leaf or the root of the rhubarb is used as an extraction site. Oak can be used as oak, brown oak, oyster oak or oak belonging to the oak family. The organic solvent may be an alcohol having 1 to 5 carbon atoms, hexane, chloroform or ethyl acetate, preferably ethanol.

In addition, the composition for water tissues may further include other fragrances or moisturizers.

The composition for water tissue can be prepared in water tissue by containing in a ratio of 1: 0.01 to 5 by weight in the base paper, preferably, the content is appropriately adjusted according to the type of base paper and the size or use of the water tissue. The water tissue manufacturing method may be absorbed by impregnating the base paper in the composition for water tissue or by spraying the composition on the base paper. Other conventional methods may be applied to prepare water tissue.

The water tissue of the present invention improves the antimicrobial effect by using electrolyzed water, and unlike the water tissue including the conventional chemicals, as well as using forest resource extracts, significantly inhibit the growth of fecal contaminants and pathogenic food poisoning microorganisms.

Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the present invention is not limited to the following examples.

Example 1: Preparation of Forest Resource Extract

Brown oak leaf and rhododendron root dried material were purchased and then ground to powder. 500 g of each powder was placed in a flask with a reflux condenser attached vertically, and 5 kg of 70% ethanol was added at 60 ° C., followed by extracting twice for 12 hours. The extract was filtered under reduced pressure and the filtrate was concentrated using a rotary vacuum evaporator (Eyela N-N-series, Japan), lyophilized and used in the experiment.

Example 2-9 Preparation of Antimicrobial Blends

Using the oak extract and rhubarb extract prepared in the same manner as in Example 1 to prepare an antimicrobial mixture of Table 1.

Experimental group Composition ratio (wt%) Purified water Acid water Alkaline water Brown oak extract Yellow lotus extract Example 2 100 - - - - Example 3 Remaining amount - - One - Example 4 - 100 - - - Example 5 - - 100 - - Example 6 - Remaining amount - One Example 7 - - Remaining amount One Example 8 - Remaining amount - One One Example 9 - Remaining amount One One

The acidic water of Table 1 was prepared by electrolysis using an electrolytic water maker (A2, E & S first, Korea) by adding 20% NaCl to tap water, pH 2.52, redox potential is 1100 mV, dissolved oxygen amount Is 25 ppm. Alkaline water was prepared by electrolysis by adding 20% NaCl to tap water using an electrolytic water maker (A2, E & S first Korea Co., Ltd.), pH 11.2, redox potential is -800 mV, dissolved oxygen 0 ppm.

The antimicrobial mixed raw materials of Examples 2 to 9 having the composition ratios of Table 1 were prepared by mixing each composition and then stirring.

Examples 10-17 Preparation of Antibacterial Water Tissues

The antimicrobial mixed raw materials of Examples 2 to 9 were sprayed onto the tissue paper with a nebulizer, and the antimicrobial mixed raw materials of Examples 10 to 17 were prepared by absorbing the antimicrobial mixed raw materials at 3 weight times the weight of the raw paper. Thereafter, the antibacterial activity of each tissue was checked.

Comparative Example 1

A water tissue distributed in Korea (product name: JOHN Laugh Water Tissue, Sales: LG Co., Ltd.) was used as a comparative example. Comparative Example is that the natural fiber spunlace contains vitamin E and aloe, and the product properties are described as containing clean after five steps of water purification process.

Experimental Example

end. Antimicrobial Activity against Coliform Group

The antimicrobial water tissues of Examples 10 to 17 were checked for the growth of Escherichia coli group in water tissues by storage period while standing at room temperature (25 ° C.).

Escherichia coli group is generated by manure discharged from humans and animals, and is used as an indicator of pathogenic bacteria in terms of environment and health. In other words, the detection of E. coli group means that it is contaminated by manure or livestock manure, which means that pathogenic bacteria may be present. Escherichia coli group is a gram-stained negative bacillus, most of which is aerobic or breathable, and develops well in the medium and decomposes glucose and lactose to generate acids and gases. In general, since E. coli is a stronger bacterium than pathogens, it is possible to determine the reduction rate of E. coli and the presence of pathogens when chlorine disinfection of effluent, and also to determine the purification function when treating sewage. The allowable limit for manure treatment plants and the like is set at 3000 pieces / cm 3.

Each antimicrobial tissue was placed in a homogenized bag (stomacher bag) with sterilized peptone water and stirred for 120 seconds, followed by dilution. Dilutions were plated with 0.1 ml each of plate count agar (PCA) plate media. E. coli group was measured using a 3M Petri film after incubation for 24 hours at 35 ℃ in PCA medium. The experimental results are shown in Table 2 below.

wet tissue Coliform count, log CFU / g 0 day log CFU / g 1 day 2 days 3 days Example 10 2.1 2.5 3.83 3.35 Example 11 1.86 2.2 2.57 3.03 Example 12 ND ND ND ND Example 13 ND ND ND ND Example 14 ND ND ND ND Example 15 ND ND ND ND Example 16 ND ND ND ND Example 17 ND ND ND ND Comparative Example 1 ND ND ND ND * ND: Not Detected

In Table 2, the E. coli group was not detected in the water tissues of Examples 10 to 17, and the E. coli group was detected only in Examples 10 and 11 treated with purified water and brown oak extract, respectively. In addition, E. coli group was not detected in Examples 12 and 13 treated with acidic and alkaline water, respectively, and it was confirmed that the use of electrolytic water rather than purified water for water tissue was a method that can effectively prevent microbial contamination.

I. Antimicrobial activity against other microorganisms

The antimicrobial water tissues of Examples 10 to 17 were confirmed at the room temperature (25 ℃) while the growth of microorganisms in the water tissues by storage period.

Each antimicrobial tissue was placed in a homogenized bag (stomacher bag) with sterilized peptone water and stirred for 120 seconds, followed by dilution. Dilutions were plated by 0.1 ml on plate count agar (PCA) plates and potato dextrose agar (PDA) plates. PCA was placed at 35 ° C for 24 hours to measure general bacteria, and PDA was placed at 25 ° C for 48 hours to measure yeast and mold counts. After the incubation, the microbial flora was expressed in log CFU / g counting the number of colonies.

The results of measuring the change in the total number of bacteria per hour in the antimicrobial tissues of Examples 10 to 17 are shown in Table 3 and FIG. 1.

wet tissue log CFU / g 0 1 day 2 days 3 days Example 10 7.37 7.6 7.71 7.77 Example 11 7.14 7.43 7.51 7.78 Example 12 2 5.22 5.44 6 Example 13 2.48 5.51 6.62 6.85 Example 14 2.3 3.41 3.63 4.75 Example 15 3.51 4.86 6.51 6.65 Example 16 0 2.3 4.25 5.03 Example 17 3.3 3.56 5.35 5.4 Comparative Example 1 0 1.2 3.3 4.2

In Table 3 and FIG. 1, a large amount of microorganisms were detected in Example 12, which was treated with purified water, and Example 13, which was treated only with brown oak extract, and in Example 14 and 15, which treated acidic and alkaline water, respectively, the microorganisms were remarkably marked. Reduced. That is, the use of acidic water or alkaline treatment is effective for the antimicrobial activity of microorganisms.

The best antibacterial effect was the water tissue of Example 14 treated with acidic water + 1% larch extract. In addition, Examples 16 and 17 also had excellent antimicrobial activity compared to the purified water treatment group, and significantly inhibited the microbial growth at 10 ³ CFU / g compared to the control 10 ⁷ CFU / g after 3 days of storage.

In the antimicrobial tissue tissues of Examples 10 to 17, the results of measuring the bacterial counts of yeast and mold over time are shown in Table 4 and FIG. 2.

wet tissue log CFU / g 0 1 day 2 days 3 days Example 10 7.44 7.48 7.56 8.85 Example 11 6.8 7.03 7.57 8.89 Example 12 0 5.43 5.76 6.15 Example 13 3.57 4.27 6.04 6.29 Example 14 3.3 4.9 5.58 5.79 Example 15 2.7 4.93 5.5 6.08 Example 16 2.3 3.86 5.03 5.24 Example 17 3.3 3.53 4.08 4.57 Comparative example 0 0 2.6 3.48

In Table 4 and Figure 2, in Example 10 treated only with purified water and Example 11 treated only with brown oak extract, a large amount of yeast and mold were detected, in particular alkaline water + 1% brown oak extract + 1% rhubarb extract The water tissue of Example 17 was very good antibacterial effect. In addition, the water tissues of Examples 12 to 17 treated with electrolytic water showed high antimicrobial effect compared to Example 10 treated with purified water.

In the case of water tissues prepared with acidic and alkaline water, both general bacteria, yeasts and molds showed less bacterial counts than those made with alkaline water. Compared with the water tissue of Comparative Example 1, it is thought that a commercially available water tissue using acidic or alkaline water with a difference of about 10 ¹ CFU / g can show a greater microbial inhibitory effect.

All. E. coli measurement

E. coli was measured after incubation at 35 ° C. for 24 hours using 3M Petri film while stored at room temperature (25 ° C.) for 3 days.

As a result, E. coli was not measured in both the water tissues of Examples 10 to 17 and the water tissues of Comparative Example 1.

Therefore, the water tissue prepared using acidic or alkaline water showed high antibacterial effect in all groups of bacteria, yeast, mold and E. coli compared to the water tissue prepared with purified water only. If it is not visible, it can be used as a new antibacterial tissue because it can be prevented from contaminants or microorganisms by manufacturing water tissue using acidic and alkaline water.

la. Determination of antimicrobial activity against Listeria monocytogenes

1 ml of Listeria monosytogenes ATCC 19111 was inoculated into 10 ml of sterile TSB (tryptic soy broth) medium, pre-incubated at 35 ° C. for 24 hours, and then centrifuged to collect cell pellets and then diluted appropriately. Samples of Comparative Examples 2 to 4 and Example 10 (water tissue prepared with purified water), Example 14 (acidic water + 1% oak extract), which were distributed so that the initial concentration of the test bacteria was 1 × 10 ⁵ CFU / mL, and Example 16 (acidic water + 1% oak extract + 1% rhubarb extract) was inoculated in water tissues and then grown at 25 ° C and observed growth.

Comparative Example 2 used is Yuhan Kimberley Co., Ltd. product name is white feminine cleansing wipes soluble in water, described as a main ingredient, spunlace non-woven fabric, purified water, skin moisturizer, cleaning agent, and has the following product characteristics : Mild and gentle pH5.5 cleaning ingredient, well-recorded in water, colorless, non-fluorescent, fragrance-free, non-alcoholic products, natural pulp, soft natural tissue wipes, and clean water after 6 steps of water purification.

Comparative Example 3 is a product name sold by Yuhan-Kimberly Co., Ltd., Kleenex S & N Wet Wipes, and is used for cleansing the skin, and has been described as having the following product characteristics. Keeps skin healthy, non-alcoholic, non-fluorescent, and non-pigmented, with no irritation to the skin, less stickiness after brushing, and a refreshing, soft natural texture.

Comparative Example 4 is a product name sold by Dongsan C & G Co., Ltd., Wet Beige Wipes, and its purpose is to change diapers when going out with a baby, and to wipe the baby's face, hands and feet. Its characteristics are as follows: It contains 99% pure plant ingredients, so it can be used safely on soft baby skin.

Antibacterial effect against Listeria in each tissue of water is shown in Table 5 and FIG.

wet tissue log CFU / g 0 1 day 2 days 3 days Example 10 4.41 4.35 4.56 4.39 Example 14 4.27 3.92 0 0 Example 16 4.13 3.51 0 0 Comparative Example 2 4.76 2.84 0 0 Comparative Example 3 4.66 0 0 0 Comparative Example 4 4.54 0 0 0

As a result, even if the storage days were increased only in water tissues prepared with purified water, the effect of reducing Listeria was not observed, and all other water tissues showed antimicrobial effect against Listeria. In addition, Comparative Examples 3 and 4 of the water tissues in circulation were found to be killed Listeria bacteria after 1 day of storage, Example 14 and 16 and Comparative Example 2 prepared with acidic water were all Listeria bacteria after 2 days of storage The death was observed. As a result, compared to the water tissue prepared by adding the forest resources to the water tissues and acidic water distributed in the purified water after 6 stages of water purification, and the antibacterial substance was added, only the forest resources were added to the acidic water. It is thought that it is possible to manufacture water tissue using acidic water and forest resources because of high killing effect in tissue.

As described above, the water tissue of the present invention improves the antibacterial effect by using electrolyzed water, and unlike the water tissue including the conventional chemicals, it not only uses plant extracts but also significantly suppresses the growth of fecal indicator bacteria and pathogenic food poisoning microorganisms. do.

Claims (6)

Antibacterial composition for water tissue containing 0.1 to 20% by weight of oak extract and the balance of electrolyzed water. The antimicrobial composition of claim 1, wherein the electrolyzed water is acidic or alkaline water. The antimicrobial composition of claim 2, wherein the acidic water has a pH of 2.5 to 2.7 and a redox potential (ORP) of 600 to 1200 mV. The antimicrobial composition of claim 2, wherein the alkaline water has a pH of 11.0 to 11.5 and a redox potential (ORP) of −850 to −50 mV. The method of claim 1. Antibacterial composition for water tissue that further comprises 0.1 to 20% by weight of the yellow lotus extract. A water tissue prepared by containing the antimicrobial composition for water tissue according to any one of claims 1 to 5 in a base paper.

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