JPH07238004A - Microbicidal sterilizer - Google Patents

Abstract

PURPOSE:To obtain an odorless and pale yellow microbicidal sterilizer pot containing an organic substance, free from any change due to light, temperature, etc., and stable for a long period by using an aqueous solution composed of a solution containing a group of minerals derived from an are belonging to mica as the main component. CONSTITUTION:The ore belonging to mica is obtained, e.g. by pretreating rotten granite which is weathered granite as necessary, subsequently allowing a mineral acid to act on it and further separating it. An aqueous solution composed of a solution containing a group of minerals derived from this ore belonging to mica is used as the main component to obtain the objective microbicidal sterilizer. The aqueous solution shows preferably properties capable of generating OH radicals in water and oxidizing organic and inorganic substances in the aqueous solution. The solution containing a group of minerals derived from the ore belonging to mica contains 1 to 20g/l aluminium, 1 to 20g/l iron, 1 to 10g/l Mg and 1 to 10g/l calcium as the main components and has 1.1 specific gravity and pH 0.1 to 2.0. As the metal pomponents in the solution containing a group of minerals, Al, Fe, Mg and K are abundantly contained.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sterilizing / disinfecting agent, and more particularly to a sterilizing / disinfecting agent containing an aqueous solution of a mineral-containing group solution derived from a mica-based mineral as a main component.

[0002]

2. Description of the Related Art Conventionally, various chemicals have been known as disinfectants. For example, as alcohols, ethyl alcohol and isopropyl alcohol are known. Since ethyl alcohol has a bactericidal action against vegetative bacteria, tubercle bacilli, rickettsia, and many viruses, it is used in addition to hand disinfection, syringes, and skin disinfection during surgery. Used for disinfecting insoluble materials. Isopropyl alcohol has a stronger bactericidal activity and is less volatile than ethyl alcohol, but it tends to ruin the skin of fingers. Examples of phenols include phenol, cresol, chlorhexidine, and hexachlorophene. Phenol is used for disinfection of instruments, fingers, excretions, and also for preservation of serum and vaccine. The mixture of cresol and potassium soap is also used for disinfecting fingers and equipment, and disinfecting sputum and feces, but it is irritating to the skin. Chlorhexidine is characterized by low skin irritation but low efficacy, and is used for disinfecting fingers, instruments, and skin, as well as for cleaning hospital rooms. Hexachlorophene is effective against Gram-positive bacteria and is used for hand disinfection in combination with a surfactant or in soaps and cosmetics. As halogens, chlorine gas, sodium hypochlorite, chlorchlore, chloramine T, iodine / iodine solution, tincture of iodine, and iodine hole are known, and chlorine gas is mainly used for disinfection of tap water. Although sodium is effective against viruses in addition to general bacteria, it has the characteristic of being corrosive to metals and irritating to skin and mucous membranes, and is used for sterilizing drinking water, pools, and equipment.
Chlor Kalky is made by quick-lime absorption of chlorine and is used for drinking water, vegetables, pools, etc. Chloramine T
Is effective for disinfecting wounds, ulcers, and fingers, iodine and iodine solution is disinfecting oral cavity and pharynx, tincture of iodine is sterilization during surgery, iodine is effective against general bacteria, tuberculosis bacteria, fungi and viruses, and the presence of organic substances Since the efficacy does not decrease even under
Used for disinfection of dishes, fingers, and highly contaminated instruments. Alkylating agents include formaldehyde and glutaraldehyde, which are used for rapid disinfection of fingers and various metals, and those reacted with potassium permanganate for fumigation of hospital rooms. Glutar aldecht is effective against bacteria, spores, and viruses and is stable, and is used for sterilizing surgical tools, catheters, tubes, anesthesia instruments, and endoscopes that cannot be steam sterilized. As surface-active substances, there are negative soap and positive soap. Oxidizing agents include hydrogen peroxide and potassium permanganate. Hydrogen peroxide is nascent oxygen and is effective for disinfecting and cleaning wounds, oral cavity, and pharynx. Potassium permanganate is oxygen when contacted with organic substances. It is used for wounds, skin, stomatitis, etc. As described above, the disinfectant is used for each application according to each characteristic.

[0003]

However, the disinfectants described above have drawbacks such as unpleasant odor and residual toxicity, irritation to the skin, and stains of substances. The present inventor has completed a completely novel bactericidal disinfectant which does not have the drawbacks of the above-mentioned commonly used disinfectants. The bactericidal disinfectant of the present invention is an odorless pale yellow solution, has a composition that is completely inorganic and does not contain organic matter at all, and has physical properties that are stable for a long time without any change due to light or temperature. Have.

[0004]

Means for Solving the Problems A mineral-containing group solution derived from a mica-based mineral of the present invention means a mica-based mineral, for example, a corroded granite that is a weathered body of granite, if necessary after pretreatment.
It is obtained by reacting this with a mineral acid and further separating it. The pretreatment of corrosive granite is adopted as necessary in order to allow the mineral acid to efficiently act, and can be obtained, for example, by pulverizing or firing weathered soil of granite containing mica-based minerals to an appropriate size. . The mica-based mineral used in the present invention is collected from mica, rock containing mica, or corrosive rock weathered with these. That is,
Vermiculite weathered with mica-based minerals is particularly preferred because it contains many types of minerals and has a high reaction with inorganic acids.

The treatment with mineral acid after the pretreatment may be carried out by allowing a mineral acid aqueous solution to act on the weathered soil of granite containing vermiculite to elute. As the mineral acid, sulfuric acid, hydrochloric acid or the like is used, but sulfuric acid is preferable. The mineral acid treatment is continued until the required minerals are eluted from the vermiculite. The mineral acid treatment is preferably carried out at a heating temperature if necessary. This mica-containing mineral-derived mineral-containing group solution is
For example, a sulfuric acid aqueous solution is added to a corroded granite containing vermiculite, preferably at a weight ratio of a corroded granite containing vermiculite to a sulfuric acid aqueous solution of 4: 4 to 3, and the mixture is reacted with stirring at room temperature to about 100 ° C. After the reaction,
The mineral-containing group solution of the present invention can be obtained by filtration.

The mineral acid-treated product thus obtained is separated into a solid substance and a liquid substance by solid-liquid separation.
As a means for solid-liquid separation, for example, a filter press or the like is used, and if necessary, further separation and purification are carried out. This mineral acid-treated liquid is a mineral-containing group solution derived from mica-based minerals,
It has a sterilizing effect.

The properties of the mineral-containing group solution derived from this mica-based mineral are as follows. 1. Mineral content: 20% of mineral content in terms of salt
Including the above. 2. Mineral content breakdown: (1) Contains a large amount of aluminum, iron, magnesium and potassium. (2) Contain most of phosphorus, titanium, calcium, manganese, sodium, silicon, cobalt, lithium, copper, nickel, zinc, and barium. (3) Does not contain arsenic, lead, mercury, chromium, or cadmium. 3. Specific gravity: 1.1 to 1.5 4. PH: 0.1 to 2.0 5. Actions: (1) Has an action of generating OH radicals and oxidizing organic substances and inorganic substances. (2) It has the effect of reducing water clusters. (3) It has deodorizing and deodorizing effects. (4) It has the function of aggregating suspended substances. 6. Physical Properties: (1) Odorless pale yellow liquid. (2) It is a completely inorganic substance and no organic substance. (3) It is stable for a long period without any change due to light, temperature, etc.

The mechanism of action of these substances cannot be said to be completely elucidated yet, but Si, Al, Mg, F in the mica-based minerals
Various metals such as e, K, Na (mostly transition metals)
And non-metals produce metal salts and non-metal salts with inorganic acids,
And as a result of many kinds of elements forming a double salt or complex salt with each other,
These serve as a medium to generate OH radicals having a strong oxidizing power, reduce the clusters of water molecules, and change them into active water. These are items that have already been confirmed by measurement by ESR (electron spin resonance device) and NMR (nuclear magnetic resonance device), and it is considered that diseased bacteria are sterilized, disinfected and prevented by these functions. Further, in the mineral-containing group solution derived from mica-based minerals, S
i, Al, Mg, Fe, K, Na, Ca, P, S
e, Ge, Zn, Mn, Cu, Co, Li, V, W, B
Many other trace minerals such as a, Ti and Rb are contained in good balance, and these trace minerals and their balance are useful for sterilizing, disinfecting and preventing disease-causing bacteria.

Table 1 shows a typical composition of a mineral-containing group solution derived from mica-based minerals. The main component of the mica-containing mineral-derived mineral group solution usually contains 1 to 20 g / liter of aluminum, 1 to 20 g / liter of iron, 1 to 10 g / liter of magnesium and 1 to 10 g / liter of potassium. There is.

[0010]

[Table 1]

The mineral-containing group solution derived from micaceous minerals described in detail above has a bactericidal and disinfecting effect against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, Salmonella typhi, human immunodeficiency virus, etc. It can be used for various purposes such as instruments, surgical clothes, baths, barber / cosmetic tools, and toothbrushes.

Hereinafter, the present invention will be described more specifically with reference to examples.

[0013]

Example 1 Production of Mineral-Containing Group Solution Derived from Mica-Based Minerals Weathered corroded granite containing vermiculite, which is a mica-based mineral, was selected and made into a powder of 4 mesh or less. Sulfate 1 m ³ was added to the powder 1 m ^3, 5 hours 100rp at 85 ° C.
The mixture was stirred at m (revolutions per minute). Then, this mixture was separated into a solid and a liquid by a filter and purified to obtain 1.5 m ³ of a mica-containing mineral-derived mineral-containing group solution.

[0014]

Example 2 In order to confirm that the mineral-containing group solution derived from the mica-based mineral produced in Example 1 produces OH radicals, ESR measurement was performed by the spin trap method. DMPO (5,5-dimethyl) as a radical scavenger
yrroline 1-oxide is a diluted aqueous solution (100 ppm, 10) of a mineral-containing group solution derived from mica-based minerals.
00 ppm) and measured. The result is shown in FIG. As is clear from FIG. 1, four characteristic peaks of DMPO-OH were obtained, and continuous generation of OH radicals was confirmed. ESR is measured by RE2 manufactured by JEOL Ltd.
It depends on X.

[0015]

Example 3 With respect to the mineral-containing group solution derived from the mica-based mineral produced in Example 1, changes in PH (hydrogen ion concentration) and conductivity when this solution was added to tap water were examined. The tap water used for dilution has a water temperature of 18 ° C, a pH of 7.6, and an electric conductivity of 0.
It was 148. The results are shown in FIGS. 2 and 3.

[0016]

Example 4 With respect to the mineral-containing group solution derived from the mica-based mineral produced in Example 1, the change of clusters when this solution was added to tap water was examined by ¹⁷ O-NMR. ¹⁷ O-NM
The setting conditions of R are as follows.

(Measurement conditions) Equipment used: JEOL JEOL JNM-GSX400
FT-NMR Measurement nuclide: ¹⁷ O Observation frequency: OBFRQ 54.10 MHz OBSET 80.00 MHz OBFIN 12073.8 MHz Observation frequency range: 24509.8 Hz Data points: 32768 points Pulse width: 22.0 μsec Measurement mode: SGNON Accumulation number: 500 times Filter: 12250Hz Solvent: Solvent-free Standard substance: H ₂ O Measurement temperature: 25 ° C

The results when tap water alone (collected at Oji, Kita-ku, Tokyo) are shown in FIG. 4, and the results when 100 ppm of a mineral-containing group solution derived from a mica-based mineral is added to tap water are shown in FIG. As is clear from this result, the half-width of the tap water was 132 Hz, while the half-width of the result when 100 ppm of the mineral-containing group solution derived from the mica-based mineral was added to the tap water was 74 Hz. It was shown that the clusters, which are aggregates, became smaller. It should be noted that water with smaller clusters is said to be good quality water, delicious water, or active water, and is considered to be useful water.

[0019]

[Example 4] Test of public bath tub Although the water quality standard of public bath tub varies depending on prefectures, for example, it is as follows in the Tokyo Metropolitan Ordinance. Turbidity should be 5 or less Potassium permanganate consumption should be 25 mg / ml or less Escherichia coli number should be 1 / ml or less PH is not specified. Itabashi Ward where approximately 200 people bathe every day Inner Radon Center 8
After the completion of the operation in the m ³ bath, 2 liters (250 pp) of the mica-based mineral-containing mineral group solution obtained in Example 1 was used.
m) was added, and 4 hours later, water was collected to examine Escherichia coli. As a result of the inspection, the number of E. coli was 0 / ml, and it passed the requirement that the number of coliforms in the Tokyo regulations is 1 / ml or less.

[0020]

Example 5 Test in Hotel Bathtub The same test as in Example 4 was conducted at a hotel in Taito-ku, Tokyo. A mineral-containing group derived from mica-based minerals obtained in Example 1 after collecting a sample of bath water from the bath having a bath capacity of 3 tons and the number of bathers of 100 to 150 / day and taking it as raw water on the day after bathing. Add 1 liter (333ppm) of solution and add 2
Samples were collected after 3 hours and after 3 hours and examined for turbidity and coliform count. The results are shown in Table 1. As can be seen from Table 2, the number of coliforms became 0 and the turbidity became almost 0 after 2 hours.

[0021]

[Table 2]

[0021]

Example 6 Measurement of Minimum Inhibitory Concentration (MIC) 0.1%, 1% of mineral-containing group solution derived from mica-based minerals,
An aqueous solution of 10% and undiluted solution was prepared, and 1 ml of each was placed in a Petri dish and mixed with 9 ml of the agar medium for sensitivity to prepare Example 1.
0.01-containing mineral group solution derived from mica-based minerals obtained in
%, 0.1%, 1% and 10% density plates were prepared.
The test bacterial solution was prepared based on the MIC measurement method (1980) defined by the Japanese Society of Chemotherapy, and the bacterial solution was applied to the drug-mixed plate medium one platinum loop at a time. It was cultured at 37 ° C. for 24 hours, and the minimum concentration at which no growth was observed was defined as the minimum inhibitory concentration (MIC). The results are shown in Table 3.

[0022]

[Table 3]

[0023]

Example 7 Measurement of Minimum Inhibitory Concentration (MIC) 0.1%, 1% and 10% of the mica-containing mineral-derived mineral-containing group solution obtained in Example 1 and an aqueous solution of the stock solution were prepared and 1 ml each was prepared. It was taken in a petri dish and mixed with 9 ml of potato dextrose agar medium to prepare 0.01%, 0.1%, 1% and 10% concentration plates of a mineral-containing group solution derived from mica-based minerals. The test mold spore suspension is JIS Z
Prepared according to 2911-1981, the suspension was applied to the drug-mixed plate medium one platinum loop at a time. 27 ° C,
After culturing for 7 days, the minimum concentration at which no growth was observed was defined as the minimum inhibitory concentration (MIC). The results are shown in Table 4.

[0024]

[Table 4]

[0025]

[Example 8] Bactericidal type bactericidal effect 10 ppm, 100 ppm, 1000 ppm (0.1 ppm) of the mica-containing mineral-derived mineral-containing group solution obtained in Example 1
%) 10000ppm (1%), 100000ppm
(10%) and stock solution 10000000 ppm (100
%) Aqueous solution, and sheep blood agar medium EX (Nissui Pharmaceutical Co., Ltd.) was prepared based on this. 9 ml of these concentrations of agar medium and 5 kinds of bacteria (Mcfarland No. 1
1 × 3 × 10 ⁸ cfu / ml) was mixed and the number of bacteria was measured after 24 hours. The results are shown in Table 5.

[0026]

[Table 5]

[0027]

Example 9 Staphylococcus aureus test Staphylococcus aureus (staph) having the sensitivities shown in Table 6
ylococcus aureus) to BHI (Bra
In Heart Infusion) medium was grown to obtain 100,000 cells / 10 ml of Staphylococcus aureus medium. The mineral-containing group solution derived from the mica-based mineral obtained in Example 1 was used as 50, 100, 150, 200, 250, 30.
The growth of cocci was tested by adding it to 100,000 / 10 ml of Staphylococcus aureus medium so that the concentration would be 0 ppm. The results are shown in Table 7. As can be seen from Table 6, when the mineral-containing group solution derived from the mica-based mineral was added to 250 ppm, the number of Staphylococcus aureus became 0 (zero).

[0028]

[Table 6]

Microscopic observation was carried out after 30 minutes and 60 minutes. For microscopic observation, agar salt mannitol re-inoculated and cultured at 37 ° C. for 24 hours was used. as a result,
Staphylococcus a
It was observed that the cell chamber and cell wall of Ureus) were destroyed.

[0030]

[Table 7]

[0031]

[Example 10] River water sterilization test of the Banda River The river water of the Banda River downstream of the Yodo River was collected, and the mineral-containing mineral group solution derived from the mica-based mineral obtained in Example 1 was added at 1 ppm and 4 p.
pm, 10 ppm, 40 ppm, 100 ppm, 400
ppm, 1000 ppm, 4000 ppm, 10000
It was added so as to be 0 ppm, and the bactericidal effect on coliform bacteria was examined. The results are shown in Table 8. As can be seen from Table 8, 100 pp of mineral-containing group solution derived from mica-based minerals
When it was more than m, the coliforms became negative and it was found that there was a bactericidal effect.

[0032]

[Table 8]

[0033]

Example 11 Hospital Floor A 300 ppm aqueous solution of the mineral-containing group solution derived from the mica-based mineral obtained in Example 1 was sterilized and sterilized on the hospital floor. After 30 minutes, perform 3 detection tests for general bacteria and E. coli.
When it was carried out at 0 places, no bacteria were detected in 23 places in general bacteria and 29 places in E. coli, but in the control group that was not sterilized with an aqueous solution of a mineral-containing group solution derived from mica-based minerals, the bacteria were found in all 30 places. Was detected.

[0034]

Example 12 Hand towel for business use After washing a hand towel for business use, it was immersed in a 400 ppm aqueous solution of the mineral-containing group solution derived from the mica-based mineral obtained in Example 1 and squeezed into a wrap film for storage. It was found that the odor did not develop even after one week had passed and the bacteria did not reproduce, but the hand towel which was not soaked in the aqueous solution of the mineral-containing group solution derived from the mica-based minerals had a bad odor after 3 days. Occurred and had to wash again.

[0035]

Example 13 Virus Inactivation The mineral-containing group solution derived from the mica-based mineral obtained in Example 1 and diluted with pure water three-fold in series and measles virus (100 TCI).
D50) in an equal amount and mixed at room temperature (20 ° C) for 60, 30,
After being left for 20, 10, and 0 minutes, the inactivating effect was examined using Vero cells. Irrespective of the reaction time, the measles virus was inactivated up to 243 times dilution. In this way, it was found that the virus inactivating effect of the mineral-containing group solution derived from mica-based minerals was immediate, and a certain amount of virus solution (cattle) was added to the dilution water of the mica-based mineral-containing mineral group solution. A solution containing 5% to 10% of fetal serum) was mixed, 10-fold serial dilution was performed, cells were inoculated, and CPE was observed to measure the inactivating effect. The virus used is measles virus and Nagahata strain. Human immunodeficiency virus, HI
JMH-1 as V. Herpes virus is a type-2 herpes simplex virus G strain. As the poliovirus, type 1 Sabin vaccine virus was used. First, with respect to HIV, even if the titer of HIV was 6.2 power per milliliter was mixed with pure water and assayed, the titer remained unchanged, but a mineral-containing group solution derived from mica-based minerals was added. In some cases 1.9% resulted in complete inactivation. Next, regarding HSV-2, the titer did not drop much at the final concentration of 0.2% of the mineral-containing group solution derived from the mica-based mineral obtained in Example 1, but was easily inactivated at 0.6% or more. did. When the same experiment was performed on polio, virus inactivation occurred in 16.7%.
The results are shown in Table 9.

[0036]

[Effect] The mineral-containing group solution derived from the mica-based mineral has a sterilizing / disinfecting effect, and can be used for various purposes such as surgical instruments, surgical clothes, baths, barber / cosmetics, toothbrushes.

[Table 9]

Claims (4)

[Claims] 1. A disinfectant comprising an aqueous solution of a mineral-containing group solution derived from a mica-based mineral as a main component. 2. An aqueous solution of a mineral-containing group solution derived from a mica-based mineral has a property of generating OH radicals in water and oxidizing organic substances and inorganic substances in the aqueous solution.
The disinfectant described. 3. The main component of the mica-containing mineral-derived mineral-containing group solution is 1 to 20 g / liter of aluminum, and 1 to 2
The composition contains 0 g / liter of iron, 1 to 10 g / liter of magnesium and 1 to 10 g / liter of potassium, and has a specific gravity of 1.1 to 1.5 and PH of 0.1 to 2.0. The disinfectant described. 4. A metal component of a mineral-containing group solution derived from a mica-based mineral, which contains a large amount of aluminum, iron, magnesium, potassium, and phosphorus, titanium, calcium,
The bactericidal disinfectant according to claim 1, which contains most of metals consisting of manganese, sodium, silicon, cobalt, lithium, copper, nickel, zinc and barium, and is free of arsenic, lead, mercury and chromium.