KR100545324B1 - improvement method for content of anti-cancer activated substance in ginseng genus - Google Patents

improvement method for content of anti-cancer activated substance in ginseng genus Download PDF

Info

Publication number
KR100545324B1
KR100545324B1 KR1020030059966A KR20030059966A KR100545324B1 KR 100545324 B1 KR100545324 B1 KR 100545324B1 KR 1020030059966 A KR1020030059966 A KR 1020030059966A KR 20030059966 A KR20030059966 A KR 20030059966A KR 100545324 B1 KR100545324 B1 KR 100545324B1
Authority
KR
South Korea
Prior art keywords
ginseng
temperature
hours
content
steaming
Prior art date
Application number
KR1020030059966A
Other languages
Korean (ko)
Other versions
KR20050022973A (en
Inventor
안헌식
이태섭
신은명
김선화
Original Assignee
주식회사 네오바이오
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 네오바이오 filed Critical 주식회사 네오바이오
Priority to KR1020030059966A priority Critical patent/KR100545324B1/en
Priority to AU2003284753A priority patent/AU2003284753A1/en
Priority to PCT/KR2003/002547 priority patent/WO2005020715A1/en
Publication of KR20050022973A publication Critical patent/KR20050022973A/en
Application granted granted Critical
Publication of KR100545324B1 publication Critical patent/KR100545324B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 인삼류의 항암활성물질 함량의 증진방법에 관한 것으로, 인삼류를 증숙하기 전 단계에서 수분 함량이 45-55% 정도가 되도록 건조한 후, 95-100℃의 온도에서 1-12시간 동안 1차 증삼처리시킨 다음, 50-80℃의 온도에서 수분함량이 70-80%가 되도록 2차 건조시키고, 2차 건조된 가공삼을 영하 10-30℃의 온도에서 10-30시간 동안 방치하여 주근의 섬유조직을 단리시키고, 2차로 100-120℃의 온도에서 2-12시간 동안 증삼처리한 후, 50-80℃의 온도에서 1-5시간 동안 증숙시키는 과정을 포함하는 것을 특징으로 하며, 본 발명에 의하여 간단한 공정으로 인삼류의 유효성분(항암활성물질)인 Rg1, Rg2, Rg3, 및 Rf를 주성분으로 함유하는 총 진세노사이드의 함량을 현격하게 증가시킬 수 있다.The present invention relates to a method for enhancing the anticancer active substance content of ginseng, which is dried at about 45-55% of water content before steaming ginseng, followed by 1-12 hours at a temperature of 95-100 ° C. After the red ginseng treatment, the resultant was dried secondly at a temperature of 50-80 ° C. to have a water content of 70-80%, and the secondary dried processed ginseng was left at a temperature of minus 10-30 ° C. for 10-30 hours. Isolating the fibrous tissue, and after the second and third treatment for 2-12 hours at a temperature of 100-120 ℃, and steaming for 1-5 hours at a temperature of 50-80 ℃, characterized in that the present invention By a simple process it is possible to significantly increase the total ginsenoside content containing Rg1, Rg2, Rg3, and Rf as an active ingredient (anticancer active substance) of ginseng as a main component.

인삼, 산삼, 장뇌삼, 유효성분, 항암활성물질, 냉동공정, 초음파, 원적외선Ginseng, wild ginseng, camphor ginseng, active ingredient, anticancer active substance, freezing process, ultrasonic wave, far infrared ray

Description

인삼류의 항암활성물질 함량의 증진방법{improvement method for content of anti-cancer activated substance in ginseng genus}Improvement method for content of anti-cancer active substance in ginseng

본 발명은 인삼류의 항암활성물질 함량의 증진방법에 관한 것으로, 보다 상세하기로는 인삼류에 미량 존재하는 항암활성물질인 진세노이드(Ginsenoside) Rf, Rg1, Rg2, Rg3의 함량을 증진시키는 방법에 관한 것이다.The present invention relates to a method for enhancing the anticancer active substance content of ginseng, and more particularly to a method for enhancing the content of ginsenosides Rf, Rg1, Rg2, Rg3, which are anticancer active substances present in trace amounts in ginseng. .

인삼은 오가피과(Araliaceae) Panax 속 식물로 학명이 Panax ginseng C. A. Meyer인 다년생 초본으로써 한반도 및 만주 등 아시아의 극동지역에 분포한다. 본 발명에서 인삼류라 함은 이러한 인삼 외에, 산삼, 장뇌산삼을 포함하는 의미이다.Ginseng is a plant in the genus Araliaceae Panax . It is a perennial herb with the scientific name Panax ginseng CA Meyer, and is distributed in the Far East of Asia such as the Korean Peninsula and Manchuria. In the present invention, the ginseng is meant to include wild ginseng and long ginseng, in addition to such ginseng.

가공하지 아니한 인삼을 수삼이라고 하며, 수삼에는 항암활성물질로 인정되고 있는 진세노이드(Ginsenoside) Rf, Rg1, Rg2, Rg3의 함량이 매우 낮다. 이러한 수삼을 증숙하여 홍삼으로 가공하게 되면 상기 항암활성물질의 함량이 증가하게 된다.Unprocessed ginseng is called ginseng, and ginseng has very low content of ginsenosides Rf, Rg1, Rg2, and Rg3, which are recognized as anticancer active substances. When steamed ginseng is processed into red ginseng, the content of the anticancer active substance is increased.

전통적인 홍삼제조법은 4년 이상 6년 근의 수삼을 쪄서 건조하는 방식으로 제조하는데, 수삼을 수 차례의 증삼(찜)하는 과정과 건조과정을 거쳐 가공하게 된 다. 이러한 가열처리 제조과정에서 생리활성 성분이 생성되는 것으로 수삼에서 확인되지 않는 성분이 홍삼에서 극미량으로 확인된다.Traditional red ginseng manufacturing method is produced by steaming dried ginseng of more than 4 years and 6 years, which is processed through several times of steaming and drying process. Bio-active components are produced during the manufacturing process of the heat treatment, and the components not identified in the fresh ginseng are confirmed in trace amounts in the red ginseng.

대한민국 등록특허공보 특10-228510호(발명의 명칭 : 진세노사이드 Rg3 및/또는 Rg5의 제조방법)에서는 수삼 및 백삼 등의 인삼 식물에는 거의 존재하지 않고 홍삼 중에 극미량으로 존재하는 진세노사이드 성분을 110∼180℃의 고온에서 0.5∼20 시간 동안 가열하여 함량이 증가시키는 방법에 관한 것을 특징으로 하는 것이 개시하고 있고, 대한민국 등록특허공보 특10-336086호(발명의 명칭 : 홍삼엑스 제조방법)에서는 물을 가열하여 발생시킨 증기로 수삼 또는 백삼을 증삼하는 공정, 상기 증삼된 삼을 건조시키는 공정, 상기 건조된 삼을 가열하여 중탕시켜 유효성분을 추출하는 공정을 포함하여 홍삼 엑스를 제조하는 방법에 관한 것을 특징으로 하는 것을 개시하고 있습니다. Republic of Korea Patent Publication No. 10-228510 (Invention name: ginsenoside Rg 3 and / or Rg 5 manufacturing method) in ginsenosides such as ginseng and white ginseng, rarely present in ginseng plants and present in trace amounts in red ginseng. Disclosed is a method of increasing the content by heating the component at a high temperature of 110 to 180 ℃ for 0.5 to 20 hours, Korean Patent Publication No. 10-336086 (Invention: Red ginseng extract manufacturing method ) To manufacture red ginseng x, including the step of increasing the fresh ginseng or white ginseng with steam generated by heating water, drying the red ginseng, and extracting the active ingredient by heating and drying the dried ginseng. Disclosed is characterized by the method.

또한, 한국공개특허공보 특2001-38802호(발명의 명칭 : 고급 홍삼의 제조방법)에서는 수삼을 97∼100℃까지 가열하여 30분∼2시간을 유지시킨 다음, 65∼75℃까지 냉각시키는 증삼 공정과, 냉각 후 2∼3시간 동안 1차 및 2차 건조하여 제조하는 방법에 관한 것을 특징으로 개시하고 있고, 한국공개특허공보 특2001-6852호(발명의 명칭 : 홍삼제조방법)에서는 90℃ 전후의 증기로 약 25시간 증삼 후 건조하는 방법에 관한 것을 특징으로 개시하고 있으며, 한국공개특허공보 특2003-5089허(발명의 명칭 : 흑삼과 흑미삼의 제조방법)에서는 인삼을 95∼110℃의 온도에서 8시간 증숙시킨 다음 건조기에서 60∼70℃의 온도로 건조하는 과정을 7회 이상 반복하는 방법에 관한 것을 특징으로 개시하고 있습니다.In addition, Korean Laid-Open Patent Publication No. 2001-38802 (name of the invention: manufacturing method of high-quality red ginseng) is heated ginseng to 97-100 ° C. for 30 minutes to 2 hours, and then cooled to 65-75 ° C. The process and a method for producing by drying the first and second drying for 2 to 3 hours after cooling, and disclosed in Korea Patent Publication No. 2001-6852 (name of the invention: red ginseng manufacturing method) 90 ℃ It discloses a method for drying after about 25 hours of steaming before and after the steam before and after, and the Korean Patent Publication No. 2003-5089 (Invention: Manufacturing method of black and black rice) ginseng 95 ~ 110 ℃ It is characterized by a method of repeating the process of steaming at a temperature of 8 hours and then drying at a temperature of 60 to 70 ° C. in a drier at least 7 times.

상기 한 바와 같은 선행기술들은 통상의 홍삼제조방법에 비하여 항암활성성분의 함량을 얼마 간 증가시키는 효과를 발휘하지만 여전히 증가 효과가 부족하다는 공통적인 문제점을 가지고 있다.Prior arts as described above have the effect of increasing the content of the anticancer active ingredient for some time compared to the conventional red ginseng manufacturing method, but still has a common problem that the increase effect is insufficient.

본 발명은 상기한 바와 같은 문제점을 해결하기 위한 것으로, 인삼류를 가공함으로써 항암활성물질인 진세노사이드 Rg1, Rg2, Rg3, 및 Rf를 주성분으로 함유하는 총 진세노사이드의 함량을 현격하게 증가시키는 것을 목적으로 한다.
The present invention is to solve the problems as described above, and to significantly increase the content of ginsenosides containing ginsenosides Rg1, Rg2, Rg3, and Rf as main components by processing ginseng The purpose.

본 발명은 인삼류를 홍삼으로 만들기 위하여 증숙하기 전 단계에서 수분 함량이 45-55% 정도가 되도록 건조한 후, 95-100℃의 온도에서 1-12시간 동안 1차 증삼(증기로 인삼류를 찌는 과정)처리시킨 다음, 50-80℃의 온도에서 수분함량이 70-80%가 되도록 2차 건조시키고, 2차 건조된 가공삼을 영하 10-30℃의 온도에서 10-30시간 동안 방치하여 주근의 섬유조직을 단리시키고, 2차로 100-120℃의 온도로 2-12시간 동안 증삼처리한 후, 50-80℃의 온도에서 1-5시간 동안 증숙시키는 것으로 구성된다.In the present invention, the first ginseng (the process of steaming ginseng with steam) for 1-12 hours at a temperature of 95-100 ℃ after drying to make the water content 45-55% in the step before steaming ginseng to make red ginseng After the treatment, the second dried to a water content of 70-80% at a temperature of 50-80 ℃, and the secondary dried processed ginseng was left for 10-30 hours at a temperature of minus 10-30 ℃, the fiber of the main root The tissues are isolated, secondly triturated for 2-12 hours at a temperature of 100-120 ° C. and then steamed for 1-5 hours at a temperature of 50-80 ° C.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

전통적인 홍삼의 제조방법은 '증삼'과 '식힘'과정을 9회 반복하는 것이다. 이러한 전통적인 방법은 공정이 지나치게 복잡하므로 증삼과 식힘 과정을 7회로 단축시킨 발명이 전술한 바와 같은 한국 공개특허 제2003-0005089호이다. The traditional method of preparing red ginseng is to repeat the process of 'red ginseng' and 'cooling' nine times. In this traditional method, the process of making the process too complicated and reducing the process of increasing the ginseng and cooling to seven times is Korean Patent Publication No. 2003-0005089.

본 발명에서는 2회의 증삼과 식힘과정을 거치되 특히 1차 증삼 후 냉각공정을 거친 다음 2차 증삼과정을 거침으로써 공정이 크게 단축됨과 동시에, 종래의 기술들에 비하여 항암활성물질의 함량이 현격하게 증가된다.In the present invention, the process is greatly shortened by going through two steaming and cooling processes, especially after the first steaming, and then undergoing a second steaming process, and at the same time, the content of the anticancer active substance is significantly higher than that of the conventional techniques. Is increased.

냉각공정은 영하 10-30℃의 온도에서 10-30시간 동안 방치하는 공정이며, 냉각공정을 통하여 주근의 섬유조직을 단리(절단)시킨 후 2차 증삼과정을 거침으로써 유효성분의 함량을 크게 높일 수 있다.The cooling process is a process that is left for 10-30 hours at a temperature of minus 10-30 ℃, and the fiber structure of the main roots is isolated (cut) through the cooling process, and then the secondary steaming process is performed to greatly increase the content of the active ingredient. Can be.

냉각공정의 온도가 상기 범위보다 높을 경우에는 주근의 섬유조직 단리효과가 부족하게 되며, 상기 범위보다 낮을 경우에는 더 이상의 단리효과의 향상을 보이지 않지만 에너지 비용이 증가하게 된다.When the temperature of the cooling process is higher than the above range, the isolation of the fiber tissue of the main root is insufficient, and when the temperature is lower than the above range, no further isolation effect is improved, but the energy cost is increased.

항암활성물질의 함량을 더욱 높이기 위해서는 2차 증삼과정 및 증숙과정에서 원적외선 처리 또는 초음파처리를 할 수 있다. 초음파처리방법은 원적외선 처리방법에 비하여 항암활성물질 함량 증진효과가 더욱 우수하지만 조직을 파괴하므로 원형이 그대로 유지되어야만 하는 홍삼의 제조공정에는 적용시킬 수 없으며, 가공 후 유효성분을 추출하든가 분말화하는 경우에는 적용시킬 수 있다. 반면 원적외선 처리방법은 조직을 파괴하지 아니하므로 홍삼화 하는 경우에도 적용이 가능하다.In order to further increase the content of the anticancer active substance, far-infrared or ultrasonic treatment may be performed in the second steaming process and steaming process. Ultrasonic treatment method is more effective in improving anticancer active substance content than far infrared rays treatment method, but it can't be applied to manufacturing process of red ginseng that must keep its original shape as it destroys tissue. Can be applied. On the other hand, the far-infrared treatment method is applicable to red ginseng because it does not destroy tissue.

홍삼화 하는 경우에는 제품의 부패 또는 변질을 방지하기 위하여 상기 공정에 추가하여 수분 함량을 15% 이내로 건조시키는 공정이 필요하다. In the case of red ginseng in order to prevent spoilage or deterioration of the product in addition to the above process is required to dry the moisture content within 15%.

또한, 원료가 되는 인삼류를 선택함에 있어서, 본 출원인에 의하여 기출원된 "DNA 지문분석방법을 이용한 산삼확인법(제2002-36091호)"에서 개시되어 있는 DNA 지문분석방법인 AFLP(Amplified Fragment Length Polymorphism)를 통하여 우수한 유전인자를 가진 인삼류를 선택하는 것이 바람직하다.In addition, in selecting ginseng as a raw material, AFLP (Amplified Fragment Length Polymorphism), which is a DNA fingerprint analysis method disclosed in the "Ginseng Identification Method Using DNA Fingerprint Analysis Method (2002-36091)," filed by the applicant. It is desirable to select ginseng having excellent genetic factors through).

본 발명의 실시예는 아래와 같다.An embodiment of the present invention is as follows.

실시예 1 Example 1

*장뇌산삼의 주요생리활성성분인 Rg1, Rg2, Rg3, 및 Rf를 주성분으로 함유하는 총 진세노사이드의 수율증대를 위한 추출물의 제조방법* Preparation method of extract for increasing the yield of total ginsenoside containing Rg1, Rg2, Rg3, and Rf, the main physiologically active ingredients of camphor ginseng

가압증기멸균기에 10년근 장뇌산삼을 수삼상태에서 50% 건조한 후 100g 정량하여 담고 95-100℃의 온도에서 6시간 동안 1차 증삼한 후, 60℃가 유지되는 온풍건조기내에서 수분함량 75%상태로 건조하였다. 1차 가공 건조된 삼을 영하 20℃ 내외로 유지되는 냉동고에서 24시간 방치하여 주근의 섬유조직을 단리(절단)시키고, 2차로 110℃의 고온에서 6시간 동안 증삼한 후 70℃ 전후에서 2시간 동안 증숙과정을 거쳐 최종 산물인 홍삼을 수득하였다. 얻어진 홍삼을 주정(발효에탄올)과 물을 각 70% : 30%의 비율로 조제한 추출액에 넣고 70℃의 온도로 유지되는 추출기에 옮겨 담고 24시간동안 교반시켜 수분을 증발시키는 상태로 유효성분을 추출하였다. 50% dried 10-year-old camphor ginseng in a pressurized steam sterilizer, and weighed 100g after primary steaming for 6 hours at a temperature of 95-100 ℃, followed by water content 75% in a warm air dryer maintained at 60 ℃. Dried over. Primary processing The dried ginseng is left (cut) for 24 hours in a freezer maintained at minus 20 ℃ and then isolated (cut). Secondly, it is steamed for 6 hours at 110 ℃ and then 2 hours at 70 ℃. After steaming, the final product red ginseng was obtained. The red ginseng obtained in alcohol (fermented ethanol) and water in the extract prepared in the ratio of 70%: 30% each, transferred to an extractor maintained at a temperature of 70 ℃, stirred for 24 hours to extract the active ingredient in the state of evaporating moisture It was.

얻어진 추출액의 유효성분 함량은 아래의 표1과 같았다.The active ingredient content of the obtained extract was as Table 1 below.

실시예 2 Example 2

*일반재배인삼의 생리활성성분인 Rg1, Rg2, Rg3, 및 Rf를 주성분으로 함유하 는 총 진세노사이드의 수율증대를 위한 추출물의 제조방법 * Preparation method of extract for increasing the yield of total ginsenosides containing Rg1, Rg2, Rg3, and Rf, the main active ingredients of general cultivated ginseng

일반재배인삼에서도 생리활성성분의 진세노사이드 함량을 증대시켰으며, 실시예 1의 장뇌산삼 대신에 4-6년근 일반재배인삼을 사용한 것을 제외하고는 실시예 1과 동일한 방법으로 유효성분을 추출하였다.Ginsenosides content of physiologically active ingredients was also increased in general cultivated ginseng, and the active ingredient was extracted in the same manner as in Example 1 except that 4-6 year-old general cultivated ginseng was used instead of the camphor ginseng of Example 1. .

실시예 3 Example 3

실시예 2와 동일하게 시행하되, 교반이 완료된 추출액에 2시간 동안 초음파 처리를 하여 조직 내의 고분자 등의 원자간의 결합이 끊어지도록 유도하였다.In the same manner as in Example 2, the extraction was stirred for 2 hours sonication was induced to break the bond between atoms such as polymer in the tissue.

실시예 4 Example 4

*장뇌산삼을 이용한 장뇌홍삼(홍장뇌삼)의 제조방법 * Manufacturing method of camphor red ginseng (hongjang brain ginseng) using camphor mountain

가압증기멸균기에 장뇌산삼을 모양을 다듬어서 담고 95-100℃의 온도에서 6시간 동안 증삼한 후, 60℃의 온도로 유지되는 온풍건조기내에서 수분함량 75% 상태로 건조하였다. 1차 가공 건조된 삼을 영하 20℃ 내외의 온도로 유지되는 냉동고에서 24시간 방치하여 주근의 섬유조직을 단리(절단)시키고 다시 모양을 정돈하여 2차로 110도의 고온에서 6시간 동안 증삼한 다음, 70℃ 전후의 온도에서 2시간 동안 원적외선을 조사하면서 증숙시켰으며, 이어서 수분 함량이 10% 미만이 되도록 건조하여 최종 제품을 얻었다.The autologous sterilized ginseng was trimmed in a pressurized steam sterilizer, and then steamed for 6 hours at a temperature of 95-100 ° C., and dried in a warm air dryer maintained at a temperature of 60 ° C. to 75% moisture content. The first processed dried ginseng is left for 24 hours in a freezer maintained at a temperature of minus 20 ℃, isolating (cutting) and reshaping the fibrous tissue of the main roots and re-shape again and then steamed for 6 hours at a high temperature of 110 degrees. Steaming was carried out for 2 hours while irradiating far-infrared radiation at a temperature of about 70 ° C., followed by drying to a moisture content of less than 10% to obtain a final product.

이 최종 제품(홍장뇌삼)의 유효성분을 분석하기 위하여 실시예 1에서의 방법과 같은 방법으로 유효성분을 추출하였다.In order to analyze the active ingredient of this final product (red ginseng brain ginseng), the active ingredient was extracted in the same manner as in Example 1.

비교예 1 Comparative Example 1

실시예 2와 동일하게 시행하되, 냉동과정을 생략하고 2차 증숙온도를 120℃로 높혔다.The same procedure as in Example 2, but the refrigeration process was omitted and the secondary steaming temperature was increased to 120 ℃.

비교예 2 Comparative Example 2

실시예 2와 동일하게 시행하되, 냉동과정을 생략하고 2차 증숙온도를 98℃로 낮추었다.In the same manner as in Example 2, but the refrigeration process was omitted and the secondary steaming temperature was lowered to 98 ℃.

<표 1>TABLE 1

GinsenosidesGinsenosides 수삼Fresh ginseng 비교예1Comparative Example 1 비교예2Comparative Example 2 장뇌삼(수삼)Camphor ginseng 실시예1Example 1 실시예2Example 2 실시예3Example 3 실시예4Example 4 Rg3Rg3 0.0100.010 1.321.32 0.240.24 미량a very small amount 12.9012.90 1.751.75 3.063.06 3.963.96 Rg2Rg2 0.0720.072 0.300.30 0.200.20 0.330.33 6.216.21 1.041.04 0.970.97 1.711.71 Rg1Rg1 1.0201.020 0.220.22 0.350.35 0.760.76 1.001.00 0.830.83 1.041.04 1.221.22 RfRf 0.5250.525 0.100.10 0.120.12 0.450.45 1.611.61 0.680.68 0.660.66 0.980.98 ReRe 0.4420.442 0.020.02 0.300.30 1.111.11 1.231.23 0.280.28 0.740.74 0.430.43 RdRd 0.2380.238 0.140.14 0.270.27 0.190.19 0.860.86 0.300.30 0.480.48 0.370.37 RcRc 0.6040.604 0.170.17 0.570.57 0.580.58 0.370.37 0.460.46 0.370.37 0.220.22 Rb2Rb2 0.3310.331 0.100.10 0.440.44 0.490.49 0.630.63 0.390.39 0.690.69 0.610.61 Rb1Rb1 0.9280.928 0.120.12 0.500.50 1.011.01 1.161.16 0.570.57 0.840.84 1.031.03 합계Sum 4.174.17 2.492.49 2.992.99 4.924.92 25.9725.97 6.36.3 8.858.85 10.5310.53

* 상기 표1의 함량은 조사포닌 100g 중의 유효성분의 함량을 나타낸 것이며, 함량측정방법은 HPLC를 통하여 측정하였다.* The content of Table 1 shows the content of the active ingredient in 100 g of irradiated phononine, the content measurement method was measured through HPLC.

본 발명에 의하여 간단한 공정으로 인삼류의 유효성분(항암활성물질)인 Rg1, Rg2, Rg3, 및 Rf를 주성분으로 함유하는 총 진세노사이드의 함량을 현격하게 증가시킬 수 있다.According to the present invention, the content of ginsenosides containing Rg1, Rg2, Rg3, and Rf, which is an active ingredient (anticancer active substance) of ginseng as a main component, can be significantly increased by a simple process.

Claims (3)

인삼류를 증숙하기 전 단계에서 수분 함량이 45-55% 정도가 되도록 건조한 후, 95-100℃의 온도에서 1-12시간 동안 1차 증삼처리시킨 다음, 50-80℃의 온도에서 수분함량이 70-80%가 되도록 2차 건조시키고, 2차 건조된 가공삼을 영하 10-30℃의 온도에서 10-30시간 동안 방치하여 주근의 섬유조직을 단리시키고, 2차로 100-120℃의 온도에서 2-12시간 동안 증삼처리한 후, 50-80℃의 온도에서 1-5시간 동안 증숙시키는 과정을 포함하는 것을 특징으로 하는 인삼류의 항암활성물질 함량의 증진방법.Before steaming ginseng, the water content is dried to about 45-55%, and then first treated for 1-12 hours at a temperature of 95-100 ℃, and then the water content is 70 at a temperature of 50-80 ℃. Secondary drying to -80%, and the second dried dried ginseng is left for 10-30 hours at a temperature of minus 10-30 ℃ to isolate the fiber tissue of the roots, and secondly at a temperature of 100-120 ℃ The method of enhancing the anticancer active substance content of ginseng, which comprises a process of steaming for 1-5 hours at a temperature of 50-80 ° C. after -12 hours of steaming treatment. 삭제delete 삭제delete
KR1020030059966A 2003-08-28 2003-08-28 improvement method for content of anti-cancer activated substance in ginseng genus KR100545324B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1020030059966A KR100545324B1 (en) 2003-08-28 2003-08-28 improvement method for content of anti-cancer activated substance in ginseng genus
AU2003284753A AU2003284753A1 (en) 2003-08-28 2003-11-25 Method of improving the content of anticancer substances in ginseng
PCT/KR2003/002547 WO2005020715A1 (en) 2003-08-28 2003-11-25 Method of improving the content of anticancer substances in ginseng

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020030059966A KR100545324B1 (en) 2003-08-28 2003-08-28 improvement method for content of anti-cancer activated substance in ginseng genus

Publications (2)

Publication Number Publication Date
KR20050022973A KR20050022973A (en) 2005-03-09
KR100545324B1 true KR100545324B1 (en) 2006-01-24

Family

ID=34270624

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020030059966A KR100545324B1 (en) 2003-08-28 2003-08-28 improvement method for content of anti-cancer activated substance in ginseng genus

Country Status (3)

Country Link
KR (1) KR100545324B1 (en)
AU (1) AU2003284753A1 (en)
WO (1) WO2005020715A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101068163B1 (en) 2009-06-30 2011-09-28 신현중 A Process for Preparing Panax Ginseng Product with Improved Pharmacological Activity
US20130324486A1 (en) * 2011-03-08 2013-12-05 Hong Sik Lee Method for producing cultured root of purple ginseng
KR20220039579A (en) 2020-09-22 2022-03-29 김진용 Method for manufacturing Panax genus plant processed product

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100762965B1 (en) 2006-03-21 2007-10-04 바이오스펙트럼 주식회사 Preparation methods of Panax ginseng extract increased contents of physiologically active substances Rb1, Rb2, Rc and Rd
EP2031980B1 (en) * 2006-05-12 2010-06-02 Jung Won Lee Method of making a black-ginseng and a condensed black-ginseng and a black-ginseng preserved in honey
JP2008100999A (en) * 2006-10-18 2008-05-01 In-Hwan Seong Process for producing ginseng fruit and ginseng peduncle having high content of ginsenoside component
CN106556658A (en) * 2016-11-17 2017-04-05 鲁东大学 A kind of method of content of ginsenoside in raising Folium Ginseng
CN115487215A (en) * 2022-09-27 2022-12-20 成都大学 Ginseng radix Rubri processing method capable of simultaneously increasing ginsenoside Rb2, rd and Rh4 content

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100192678B1 (en) * 1995-06-07 1999-06-15 손경식 Processed ginseng product having an increased pharmacological activity
KR0183448B1 (en) * 1996-05-31 1999-05-01 손경식 Anti-carcinogenic composition containing ginsenoside rg5
KR100471340B1 (en) * 2003-11-29 2005-03-14 롯데제과주식회사 A new process for enhancing the efficacies of Ginseng

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101068163B1 (en) 2009-06-30 2011-09-28 신현중 A Process for Preparing Panax Ginseng Product with Improved Pharmacological Activity
US20130324486A1 (en) * 2011-03-08 2013-12-05 Hong Sik Lee Method for producing cultured root of purple ginseng
US9585904B2 (en) * 2011-03-08 2017-03-07 Hong Sik Lee Method for producing cultured root of purple ginseng
KR20220039579A (en) 2020-09-22 2022-03-29 김진용 Method for manufacturing Panax genus plant processed product

Also Published As

Publication number Publication date
WO2005020715A1 (en) 2005-03-10
AU2003284753A1 (en) 2005-03-16
KR20050022973A (en) 2005-03-09

Similar Documents

Publication Publication Date Title
KR101325832B1 (en) BLACK GINSENG HAVING EXCEL CONTENT OF GINSENOSIDE Rh2 INGREDIENT AND THE CONCENTRATE OF BLACK GINSENG AND BLACK GINSENG GOODS MANUFACTURED BY THE SAME
KR100545324B1 (en) improvement method for content of anti-cancer activated substance in ginseng genus
JP7350137B2 (en) Method for producing black ginseng concentrate with increased prosapogeninsenoside content using continuous evaporation and combined concentration technology
US20080113043A1 (en) Method for producing ginseng fruit and ginseng flower stalk with high content of ginsenoside
CN101780128B (en) Preparation method of brown ginseng
KR100844454B1 (en) Beverage of ginseng fruit and manufacturing method thereof
KR101598267B1 (en) Hwangchil ginseng impregnated with Hwangchu tree extract and its preparation method
KR20100043725A (en) Changes of protopanaxadiol ginsenosides in ginseng leaves by steaming heat treatment
KR100840490B1 (en) Method of manufacturing white ginseng and red ginseng by extra-high pressure treatment
KR101068216B1 (en) Method for producing dried ginseng with increased red ginseng components using far-infrared irradiation
KR100852137B1 (en) Manufacturing method of ginseng fruit and ginseng spray plenty ginsenoside quantity
KR101563930B1 (en) mountain-ash grain syrup and taffy and the manufacturing method thereof
KR100753771B1 (en) The manufacturing process of the black-ginseng and black-ginseng concentrate
KR102295116B1 (en) Manufacturing method of black ginseng to improve cognitive function and black ginseng manufactured by the method
KR20100043723A (en) Changes of protopanaxadiol ginsenosides in ginseng leaves by far infrared heat treatment
KR100336086B1 (en) Ginseng extract make method
KR100857767B1 (en) Manufacturing method of ginseng extract from ginseng fruit
KR20220001825A (en) Method for preparing red ginseng extract and red ginseng extract prepared by using the method
KR101413111B1 (en) Manufacturing method of red ginseng pure drinkable
KR101057226B1 (en) Method of manufacturing ginseng with reduced fragrance components using far infrared ray irradiation
KR102665525B1 (en) The method and the extract of extracting ginsenosides from red ginseng or black ginseng, manufactured using ultra-high pressure treatment, using high-temperature and high-pressure water
KR101068163B1 (en) A Process for Preparing Panax Ginseng Product with Improved Pharmacological Activity
KR101084416B1 (en) Production of Dried Ginseng with Increased Antioxidant Activity Using Far-infrared
KR20240074974A (en) Method for preparing red ginseng extract having high content of ginsenoside and red ginseng extract prepared by using the method
KR101908766B1 (en) Manufacturing method for the slice of red ginseng

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E90F Notification of reason for final refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20090112

Year of fee payment: 4

LAPS Lapse due to unpaid annual fee