CN106556658A - A kind of method of content of ginsenoside in raising Folium Ginseng - Google Patents
A kind of method of content of ginsenoside in raising Folium Ginseng Download PDFInfo
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- CN106556658A CN106556658A CN201611024775.8A CN201611024775A CN106556658A CN 106556658 A CN106556658 A CN 106556658A CN 201611024775 A CN201611024775 A CN 201611024775A CN 106556658 A CN106556658 A CN 106556658A
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Abstract
The present invention relates to a kind of method for improving content of ginsenoside in Folium Ginseng, including by Folium Ginseng cleaning, be dried, crush, the step such as irradiation, organic solvent extraction under far infrared, the invention has the beneficial effects as follows：1) transformation efficiency height, speed are fast, and yield is high；2) physical transformations method, free from environmental pollution, environmental protection.
Description
Technical field
The present invention relates to ginsenoside in a kind of method for improving content of ginsenoside, more particularly to a kind of raising Folium Ginseng
The method of content, belongs to natural product conversion and extractive technique field.
Background technology
It has often been said that Radix Ginseng be Araliaceae Radix Ginseng (Panax ginseng C.A.Meyer) dry root, its medicine
With with a long history, it is the valuable resources of medicinal plant of China, its research and application have been subject to most attention both domestic and external.With people
To Radix Ginseng research deeply and develop, Chemical Constituents of Panax Ginseng and its pharmacological action are gradually found.Radix Ginseng contains variousization
Study point, such as ginsenoside, ginseng polysaccharide, Panaxynol, ginseng essential oil, aminoacid, alkaloid, polypeptide and trace element
Deng wherein ginsenoside is the significant composition of Radix Ginseng, is the glucoside compound that constitutes of being connected by sapogenin and sugar, total saponins
Content is about 4%.Modern pharmacology research shows that the principle active component in Radix Ginseng is ginsenoside, and its physiologically active is extremely
Extensively.Ginsenoside has resisting fatigue, improves memory, slow down aging, adjust nervus centraliss, improve body's immunity, change
The multiple pharmacological effects such as mercy blood vessel blood supply insufficiency, antitumor.
Ginsenoside in Radix Ginseng is different because the position of Radix Ginseng, processing method, Cultivation time and place of production etc. are different, different
The species and content of the ginsenoside in panax species also have very big difference.Ginsenoside contained by each position of panax ginseng plant
Species, content are different from, and in Radix Ginseng, total saponin content accounts for the 2.0～7.0% of dry weight, and in Folium Ginseng, total saponin content is about
For 7.6～12.6%, alabastrum total saponin content 14～15%, in tame Radix Ginseng, the content of ginsenoside is with growth year
And accumulate, the saponin content of usual 4～5 years strangers ginseng reaches highest.
Ginsenoside belongs to dammarane type four-ring triterpenoid class compound, can be divided into original by the structure difference of its aglycon part
Panoxadiol's type saponin, Protopanaxatriol's type saponin and oleanolic acid.In ginsenoside, some Hydrolizates have
The higher pharmacologically active of more primary saponin, such as ginsenoside Rg3, Rh2, Rh4, Rk1, Rg5 etc., as these ginsenosides derive
Thing is substantially not present in tame Radix Ginseng, therefore is referred to as rare ginsenoside.Newest pharmaceutical research shows, these
Rare ginsenoside, is reversed white for apoptotic protection with uniqueness pharmacologically active, especially ginsenoside Rk1 and Rg5
Disorders of blood cellular drug resistance, prevent senile dementia, memory reinforcing, by regulatory protein kinase c activity and glucocorticoid sample
Receptor acting suppresses propagation of tumor cell etc. all to have good function.These rare ginsenosides are played as natural drug
The material base of drug effect, depends merely on traditional extraction purification method, and yield is very low, it is impossible to meet production needs.In recent years, both at home and abroad
Researcher attempts the derivant by not having in various different approaches acquisition Radix Ginseng originally or content is rare.The acquisition way of derivant
Footpath mainly has：The methods such as acid hydrolysis, basic hydrolysiss, enzymatic conversion, microorganism conversion, lactate synthesis.Although these methods are rare people
The ginseng acquisition of saponin, development and utilization provide reference, but to there is pollution environment, efficiency low, expensive for these methods
And the defect such as time length.
Far infrared is the electromagnetic wave that wavelength field is 3～30um.FAR INFRARED HEATING is imitated as a small investment, energy-conservation
The significant technology of fruit, is widely used in promoting the maturation of food, improving quality, keeps nutritional labeling, antisepsis and sterilization, dehydration
The food processing industrys such as dried.When Far-infrared spoke shines object, irradiation can be passed to atom, molecule of object etc. by absorption
Particle, makes the irregular movement of these particles more and more violent, causes then the thermogenic action and resonance motion of object, with grain
Son continues to absorb radiation energy, resonance motion is further intensified, and object also occurs chemically and physically to change therewith.
The content of the invention
The deficiency that the present invention is present for the method that existing rare ginsenoside improves content, there is provided a kind of to improve Radix Ginseng
The method of content of ginsenoside in leaf.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of method of content of ginsenoside in raising Folium Ginseng, it is characterised in that comprise the steps：1) raw material is clear
Wash：The Folium Ginseng of collection is cleaned, to remove the material remained on defoliation；
2) it is dried：Folium Ginseng after cleaning is placed at shady and cool ventilation and is dried；
3) crush：Dried leaves of panax ginseng is crushed, it is standby in loading sealing container；
4) Far-infrared spoke shines：By step 3) in the Ginseng Leaf of gained be placed under far infrared and carry out radiation treatment, after
It is placed in sealing container and preserves；
5) by step 4) in the Ginseng Leaf of gained be placed in polar organic solvent and dissolve to obtain solution；
6) concentrate, lyophilizing obtains the extract rich in ginsenoside Rk1 and Rg5.
On the basis of above-mentioned technical proposal, the present invention can also do following improvement.
Further, the wave-length coverage of the far infrared is 2-14 μm.
Further, step 4) in radiation treatment temperature conditionss be 160-200 DEG C.
Further, step 4) in irradiation time be 30min.
Further, step 5) described in polar organic solvent be alcohols solvent, described alcohols solvent is ethanol or first
Alcohol.
The invention has the beneficial effects as follows：
1) transformation efficiency height, speed are fast, and yield is high；
2) it is using physical transformations method, free from environmental pollution, environmental protection.
Description of the drawings
High-efficient liquid phase chromatograms of the Fig. 1 for standard sample Rk1；
High-efficient liquid phase chromatograms of the Fig. 2 for standard sample Rg5；
High-efficient liquid phase chromatograms of the Fig. 3 for 1 products obtained therefrom of embodiment 1-5 and comparative example；
Specific embodiment
The principle and feature of the present invention are described below in conjunction with example, example is served only for explaining the present invention, and
It is non-for limiting the scope of the present invention.
In the embodiment of the present invention, the product of gained carries out qualitative and quantitative analysis using high performance liquid chromatography, and its design parameter is such as
Under：Chromatographic column：Kenetex ODS C18(4.6×150mm,5um)；Mobile phase：A：Distilled water；B：Acetonitrile；Gradient elution program：
0-30min, 17-21%B；30-35min, 21-23%B；35-45min, 23-25%B；45-65min, 25-40%B；65-
85min, 40-65%B；85-90min, 65%-65%B；Flow velocity is 1ml/min；35 DEG C of column temperature；Detection wavelength 203nm；Sample introduction
Amount：10uL.
Jing carrying out after demarcation measurement using pure ginsenoside Rk1 and Rg5 standard sample, ginsenoside Rk1 and Rg5 are determined
Appearance time be respectively 77.687min and 78.218min.
Embodiment 1：
In a kind of raising Folium Ginseng, the method for content of ginsenoside, comprises the steps：
1) raw material cleaning, takes twice of some use clean waters of wild Koryo Rhizoma panacis majoris, except the material remained on defoliation, Yi Mianying
Ring the accuracy of experimental data；
2) it is dried：Folium Ginseng after cleaning is placed at shady and cool ventilation and is dried, and weighed, until weight twice in front and back
Till data are constant；
3) crush：Dried Folium Ginseng pulverizer is crushed to into 30～60 mesh, is placed in standby in sealing container；
4) far infrared irradiation：Ginseng Leaf after crushing is placed under 2～14 μm of far infrared in 160 DEG C of irradiation
30min, after be placed in sealing container preserve；
5) by step 4) in gained Ginseng Leaf be placed in appropriate ethanol and carry out dissolving to obtain solution；
6) resulting solution is carried out into qualitative and quantitative analysis to ginsenoside Rk1 and Rg5 using high performance liquid chromatography.
Embodiment 2：
The present embodiment with the difference of embodiment 1 is, step 4) temperature of mid and far infrared line irradiation is 170 DEG C.
Embodiment 3：
The present embodiment with the difference of embodiment 1 is, step 4) temperature of mid and far infrared line irradiation is 180 DEG C.
Embodiment 4：
The present embodiment with the difference of embodiment 1 is, step 4) temperature of mid and far infrared line irradiation is 190 DEG C.
Embodiment 5：
The present embodiment with the difference of embodiment 1 is, step 4) temperature of mid and far infrared line irradiation is 200 DEG C.
Comparative example 1：
Twice of some use clean waters of wild Koryo Rhizoma panacis majoris are taken, except the material remained on defoliation；Afterwards by the Radix Ginseng after cleaning
Leaf is placed at shady and cool ventilation and is dried, and is weighed, till weight data is constant twice in front and back；Crush：Will be dried
Folium Ginseng pulverizer is crushed to 30～60 mesh, is placed in standby in sealing container；Gained Ginseng Leaf is placed in into appropriate second
Carry out dissolving to obtain solution in alcohol；Resulting solution is carried out to ginsenoside Rk1 and Rg5 using high performance liquid chromatography qualitative and quantitative
Analysis.
By embodiment 1-5 with 1 products obtained therefrom of comparative example using the high performance liquid chromatography result such as table 1 quantitative to Rk1 and Rg5
It is shown：
Table 1：The quantitative data of ginsenoside in 1 products obtained therefrom of embodiment 1-5 and comparative example
| Rk1(mg/g) | Rg5(mg/g) | |
| Comparative example 1 | Do not detect | Do not detect |
| Embodiment 1 | 2.09 | 6.07 |
| Embodiment 2 | 3.14 | 9.06 |
| Embodiment 3 | 7.79 | 22.66 |
| Embodiment 4 | 12.27 | 34.54 |
| Embodiment 5 | 12.80 | 34.72 |
Can be seen that by the result of Fig. 1 and Biao 1 and there is no rare Radix Ginseng in the Folium Ginseng without far infrared radiation treatment
Saponin Rk1 and Rg5, but after far infrared radiation treatment, start to be converted into, and as Far-infrared spoke is according to the rising for the treatment of temperature
Increase, wherein ginsenoside Rk1 through 200 DEG C of far infrared radiation treatment content be 160 DEG C process under 6.13 times, 170 DEG C
4.08 times under processing, 1.64 times under 180 DEG C of process, 1.04 times under 190 DEG C of process；5 content of ginsenoside Rg is with remote
The rising of infrared radiation treatment temperature and increase, wherein the content through 200 DEG C of far infrared radiation treatment is 160 DEG C and processes lower
5.72 times, 3.83 times under 170 DEG C of process, 1.53 times under 180 DEG C of process, 1.01 times under 190 DEG C of process.
The foregoing is only presently preferred embodiments of the present invention, not to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (6)
1. it is a kind of improve Folium Ginseng in content of ginsenoside method, it is characterised in that comprise the steps：
1) raw material cleaning：The Folium Ginseng of collection is cleaned, to remove the material remained on defoliation；
2) it is dried：Folium Ginseng after cleaning is placed at shady and cool ventilation and is dried；
3) crush：Dried leaves of panax ginseng is crushed, it is standby in loading sealing container；
4) Far-infrared spoke shines：By step 3) in the Ginseng Leaf of gained be placed under far infrared and carry out radiation treatment, after be placed in
Preserve in sealing container；
5) by step 4) in the Ginseng Leaf of gained be placed in polar organic solvent and dissolve to obtain solution；
6) concentrate, lyophilizing obtains the extract rich in ginsenoside Rk1 and Rg5.
2. it is according to claim 1 improve Folium Ginseng in content of ginsenoside method, it is characterised in that the far infrared
The wave-length coverage of line is 2-14 μm.
3. it is according to claim 1 and 2 improve Folium Ginseng in content of ginsenoside method, it is characterised in that step 4)
The temperature conditionss of middle radiation treatment are 160-200 DEG C.
4. it is according to claim 3 improve Folium Ginseng in content of ginsenoside method, it is characterised in that step 4) in spoke
According to time be 30min.
5. it is according to claim 3 improve Folium Ginseng in content of ginsenoside method, it is characterised in that step 5) in institute
The polar organic solvent stated is alcohols solvent.
6. it is according to claim 4 improve Folium Ginseng in content of ginsenoside method, it is characterised in that described alcohols
Solvent is ethanol or methanol.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611024775.8A CN106556658A (en) | 2016-11-17 | 2016-11-17 | A kind of method of content of ginsenoside in raising Folium Ginseng |
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| CN201611024775.8A CN106556658A (en) | 2016-11-17 | 2016-11-17 | A kind of method of content of ginsenoside in raising Folium Ginseng |
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| WO2005020715A1 (en) * | 2003-08-28 | 2005-03-10 | Neo Bio Co., Ltd. | Method of improving the content of anticancer substances in ginseng |
| CN101181328A (en) * | 2006-10-18 | 2008-05-21 | 成仁焕 | Method for producing ginseng fruit and ginseng flower stalk with high content of ginsenoside |
| CN102133251A (en) * | 2011-03-15 | 2011-07-27 | 李仁� | Processing method for improving ginsenosides content in radix ginseng rubra |
| KR20110134541A (en) * | 2010-06-09 | 2011-12-15 | 재단법인 홍천메디칼허브연구소 | Method for producing dried cultivated wild ginseng with increased functional compounds contents using far-infrared irradiation |
| CN102940076A (en) * | 2012-11-08 | 2013-02-27 | 太原市素有苦荞食品有限公司 | Ginseng buckwheat tea and preparation technology of ginseng buckwheat tea |
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| CN101181328A (en) * | 2006-10-18 | 2008-05-21 | 成仁焕 | Method for producing ginseng fruit and ginseng flower stalk with high content of ginsenoside |
| KR20110134541A (en) * | 2010-06-09 | 2011-12-15 | 재단법인 홍천메디칼허브연구소 | Method for producing dried cultivated wild ginseng with increased functional compounds contents using far-infrared irradiation |
| CN102133251A (en) * | 2011-03-15 | 2011-07-27 | 李仁� | Processing method for improving ginsenosides content in radix ginseng rubra |
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| KR20130049651A (en) * | 2011-11-04 | 2013-05-14 | 주식회사 동구제약 | Method for manufacturing the red ginseng tea bag used high-vacuum art and the red ginseng tea bag made thereby |
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Application publication date: 20170405 |