KR100544607B1 - Natural disinfectant for equipment raising fish - Google Patents
Natural disinfectant for equipment raising fish Download PDFInfo
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- KR100544607B1 KR100544607B1 KR1020030078349A KR20030078349A KR100544607B1 KR 100544607 B1 KR100544607 B1 KR 100544607B1 KR 1020030078349 A KR1020030078349 A KR 1020030078349A KR 20030078349 A KR20030078349 A KR 20030078349A KR 100544607 B1 KR100544607 B1 KR 100544607B1
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- 239000000645 desinfectant Substances 0.000 title claims abstract description 45
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 42
- 239000002023 wood Substances 0.000 claims abstract description 14
- 239000000052 vinegar Substances 0.000 claims abstract description 12
- 235000021419 vinegar Nutrition 0.000 claims abstract description 12
- 241000241413 Propolis Species 0.000 claims abstract description 10
- 229940069949 propolis Drugs 0.000 claims abstract description 10
- 201000010099 disease Diseases 0.000 claims abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 8
- 239000012895 dilution Substances 0.000 claims description 77
- 238000010790 dilution Methods 0.000 claims description 77
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 238000004659 sterilization and disinfection Methods 0.000 claims description 20
- 241000607142 Salmonella Species 0.000 claims description 13
- 238000009372 pisciculture Methods 0.000 claims description 13
- 241000607471 Edwardsiella tarda Species 0.000 claims description 10
- 239000000203 mixture Substances 0.000 abstract description 6
- 238000007865 diluting Methods 0.000 abstract 1
- 239000005416 organic matter Substances 0.000 description 46
- 238000011282 treatment Methods 0.000 description 28
- 238000012360 testing method Methods 0.000 description 22
- 239000008233 hard water Substances 0.000 description 20
- 239000012153 distilled water Substances 0.000 description 18
- 230000000844 anti-bacterial effect Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 14
- 241000191967 Staphylococcus aureus Species 0.000 description 8
- 241000194056 Streptococcus iniae Species 0.000 description 8
- 241000544286 Vibrio anguillarum Species 0.000 description 8
- 244000052769 pathogen Species 0.000 description 7
- 230000001717 pathogenic effect Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 238000012827 research and development Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- RGSFGYAAUTVSQA-UHFFFAOYSA-N Cyclopentane Chemical compound C1CCCC1 RGSFGYAAUTVSQA-UHFFFAOYSA-N 0.000 description 2
- 241000607598 Vibrio Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- QTWJRLJHJPIABL-UHFFFAOYSA-N 2-methylphenol;3-methylphenol;4-methylphenol Chemical compound CC1=CC=C(O)C=C1.CC1=CC=CC(O)=C1.CC1=CC=CC=C1O QTWJRLJHJPIABL-UHFFFAOYSA-N 0.000 description 1
- 241000256837 Apidae Species 0.000 description 1
- 206010063659 Aversion Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019093 NaOCl Inorganic materials 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229930003836 cresol Natural products 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- DMEGYFMYUHOHGS-UHFFFAOYSA-N heptamethylene Natural products C1CCCCCC1 DMEGYFMYUHOHGS-UHFFFAOYSA-N 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 150000003216 pyrazines Chemical class 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 231100000456 subacute toxicity Toxicity 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 천연 소독제에 관한 것으로서, 특히 목초액과 프로폴리스가 97:3의 비율로 혼합 사용되는 것을 특징으로 한다. 이와 같은 본원 발명에 의하면, 적정한 농도로 희석하여 사용시에 질병 예방 및 치료에 효과가 있고, 환경 및 어류에 미치는 영향이 적어 혐오감을 줄일 수 있으며, 나아가, 어민 소득 향상에 기여할 수 있다.The present invention relates to a natural disinfectant, characterized in that the mixture of wood vinegar and propolis in a ratio of 97: 3. According to the present invention, by diluting to an appropriate concentration, it is effective in preventing and treating diseases at the time of use, and has a low impact on the environment and fish, thereby reducing disgust and further improving fishermen's income.
소독제, 목초액, 프로폴리스, 양어장, 천연Disinfectant, wood vinegar, propolis, fish farm, natural
Description
본 발명은 양어장용 천연 소독제에 관한 것으로서, 보다 상세하게는 양어장 등에서 위생을 위해 사용할 수 있는 목초액 및 프로폴리스로 구성된 천연 소독제에 관한 것이다.The present invention relates to a natural disinfectant for fish farms, and more particularly to a natural disinfectant composed of wood vinegar and propolis that can be used for hygiene in fish farms.
양어장이라 함은 물고기의 서식 환경을 인위적으로 조성하여 물고기를 길러 번식시키는 곳으로서, 상기 양어장과 같은 시설에서는 위생이 중요한 과제이며, 따라서, 양어장 등에서는 수중 세균 및 병원성 세균의 발생과 증식을 방지하기 위해 차아염소산나트륨(NaOCl) 용액과 같은 화학 약품으로 이루어진 화학 소독제를 이용해 시설을 소독해 왔다. A fish farm is a place where fish are artificially created to breed and breed fish. Hygiene is an important task in facilities such as fish farms. Therefore, fish farms, etc., prevent the occurrence and proliferation of aquatic and pathogenic bacteria. Hazards have been used to disinfect facilities with chemical disinfectants consisting of chemicals such as sodium hypochlorite (NaOCl) solutions.
그러나, 상기한 바와 같은 화학 소독제는 과도하게 사용하는 경우에 어류 및 인체에 좋지 않은 영향을 미치며 환경을 파괴하는 부작용이 있다는 문제점이 있었다.However, the chemical disinfectant as described above has a problem that adverse effects on fish and the human body and destroy the environment when used excessively.
본 발명은 상기한 바와 같은 문제점을 해결하기 위한 것으로서, 양어장 시설 등을 소독하는데 있어 화학 소독제의 사용을 대체 내지는 감소시키는 양어장용 천 연 소독제를 제공하는 것을 목적으로 한다.An object of the present invention is to provide a natural disinfectant for fish farms that replaces or reduces the use of chemical disinfectants in disinfecting fish farm facilities and the like.
상기한 목적을 달성하기 위한 본 발명은 양어장용 천연 소독제에 있어서, 목초액과 프로폴리스가 97:3의 비율로 혼합 사용되는 것을 특징으로 한다.The present invention for achieving the above object is characterized in that in the natural disinfectant for fish farms, wood vinegar and propolis is used in a ratio of 97: 3.
상기 목초액은 목재의 열분해에 의하여 얻어지는 것으로 약 300여종 이상의 성분이 포함되어 있으며, 셀룰로오스(cellulose), 리그닌(lignin)의 조성 비율에 따라 특성이 달라진다. 상기 목재의 다양한 성분 중 초산이 가장 높은 비율을 차지하며 그 외에, 프로피온산, 부틸산 등의 산류와 페놀, 크레졸 등 알콜류, 기타 사이클로펜탄, 아세톤, 피라진 화합물 등이 함유되어 있으며, pH 3.0의 강산성을 나타낸다.The wood vinegar is obtained by pyrolysis of wood, and contains about 300 or more components, and its properties vary depending on the composition ratio of cellulose and lignin. Among the various components of the wood, acetic acid occupies the highest ratio, and in addition, acids such as propionic acid and butyl acid, alcohols such as phenol and cresol, and other cyclopentane, acetone and pyrazine compounds are contained, and the strong acidity of pH 3.0 is contained. Indicates.
이와 같은 목초액은 자연 순환형 비료를 제조하는데 사용되며, 토양의 산도를 증가시키고, 총질소 함량, 유효인산 및 토양 내 양이온의 함량을 증가시키는 효과가 있으며, 수중세균인 에드워드균(Edwardsiella tarda) 등에 대하여 500배 희석 농도에서 살균효과가 있다.Such wood vinegar is used to produce natural circulating fertilizer, and has the effect of increasing the acidity of the soil, increasing the total nitrogen content, the effective phosphorus and the cation content in the soil, Edwardsella tarda, etc. It has a bactericidal effect at a 500-fold dilution concentration.
한편, 상기 목초액은 아급성 독성이 포함되어 있으나, 그 정도가 약하여 어류와 같은 해양동물의 생장에 큰 영향을 미치지는 않을 것으로 예견된다.On the other hand, the wood vinegar is included in the subacute toxicity, but the extent is not expected to have a significant effect on the growth of marine animals such as fish.
상기 프로폴리스는 꿀벌이 미생물 번식을 막기 위하여 분비하는 물질로서, 함곰팡이성, 항세균성 및 항바이러스 작용이 있으며, 화농성 연쇄상구균(Streptococcus pyogenes)에 살균효과가 있다.The propolis is a substance secreted by honey bees to prevent microbial growth, has fungal, antibacterial and antiviral effects, and has a bactericidal effect on Streptococcus pyogenes.
상기한 바와 같은 목초액 및 프로폴리스로 구성되는 본 발명에 의한 천연 소 독제의 효력인 살균능을 조사하기 위하여 시험용 균주인 공시 세균으로서 살모넬라콜레라스위스(Salmonella cholerasuis), 에드워드균(Edwardsiella tarda), 비브리오균(Vibrio anguillarum), 화농성 연쇄상구균(Streptococcus iniae), 황색포도상구균(Staphylococcus aureus)가 사용되며, 상기 공시 세균의 출처 및 성상을 표 1에 나타내었다.Salmonella cholerasuis (Edwardsiella tarda), Vibrio bacteria as a test strain as a test bacterium to investigate the bactericidal activity of the natural disinfectant according to the present invention composed of wood vinegar and propolis as described above (Vibrio anguillarum), Streptococcus iniae, Staphylococcus aureus are used, and the source and properties of the disclosed bacteria are shown in Table 1.
상기 시험용 균주는 동결보존되어, 평판배지에서 배양되고 영양배지(nutrient broth)에서 증식된 것으로서, 사용 직전까지 37℃로 보관되고, 시험시의 농도는 108CFU/mL이다.The test strain was cryopreserved, cultured in plate media and grown on nutrient broth, stored at 37 ° C. until just before use, and the concentration at the time of testing was 10 8 CFU / mL.
또한, 본 발명에 의한 천연 소독제의 효력을 시험하기 위한 처리구는 표 2와 같다.In addition, the treatment tool for testing the effectiveness of the natural disinfectant according to the present invention is shown in Table 2.
한편, 본 발명에 의한 천연 소독제는 0.45㎛ 필터로 여과 멸균한 후 표 3과 같이 2배씩 단계 희석하였다. On the other hand, the natural disinfectant according to the present invention was filtered and sterilized by 0.45㎛ filter, and then diluted twice stepwise as shown in Table 3.
즉, 시험관-1의 소독제의 희석배수가 2가 되도록 경수로 희석하여 희석액을 만든 후에 시험관-2에 시험관-1의 희석액 2.5㎖를 섞어 시험관-2의 소독제의 희석배수가 4배가 되도록 하며, 같은 방법으로 시험관-9까지 소독제를 희석한다. 이후, 시험관-9에서 희석액 2.5㎖를 버린 후에, 희석된 소독제가 들어있는 시험관들을 4℃에서 보관한다.That is, after dilution with hard water so that the dilution factor of test tube-1 is 2, make a dilution solution, and then mix 2.5 ml of test tube-1's diluent with test tube-2 to make the dilution factor of test tube-2 4 times. Dilute the disinfectant to Tube-9. Then, 2.5 ml of the diluent is discarded in Test Tube-9, and the test tubes containing the diluted disinfectant are stored at 4 ° C.
계속해서, 37℃로 보관된 공시 세균(108CFU/mL) 4mL를 4℃의 유기물 희석액(yeast extract) 96mL에 섞어 혼합액을 만든 후에, 혼합액 2.5mL를 꺼내어 4℃에 보관된 희석된 소독제가 들어있는 시험관에 넣고 혼합한 다음 4℃에서 정확히 30분간 반응시킨다. 이 때, 각 시험관 처리는 1분 간격으로 실시하며 10분마다 흔들어 준다.Subsequently, 4 mL of the published bacteria (10 8 CFU / mL) stored at 37 ° C. were mixed with 96 mL of 4 ° C. yeast extract at 4 ° C. to prepare a mixed solution, and then 2.5 mL of the mixed solution was taken out and the diluted disinfectant stored at 4 ° C. Place in a test tube, mix and react for exactly 30 minutes at 4 ℃. At this time, each test tube treatment is performed at 1 minute intervals and shaken every 10 minutes.
시험관별로 30분간 반응시킨 후 소독제의 효능을 중화하기 위해 즉시 각 시험관에서 1.0mL를 취하여 37℃의 9.0mL중화배지(영양배지 + 불활화 처리한 말혈청 5%)에 넣고 혼합한 다음, 각 희석액별로 0.1mL씩 취하여 영양배지가 들어있는 5개의 시험관에 넣어 혼합하고 37℃ 항온실에서 48시간 증식시킨다.In order to neutralize the efficacy of the disinfectant, after reacting for 30 minutes by tube, take 1.0 mL from each test tube immediately, mix it in 37 mL 9.0mL neutralizing medium (nutritional medium + inactivated horse serum 5%), and mix each diluted solution. Each 0.1mL is taken and mixed in 5 test tubes containing nutrient medium and grown for 48 hours at 37 ℃ constant temperature room.
상기 과정을 통해 3개의 동일 소독제 희석배수의 영양배지에서 2개 이상 증 식이 인정되지 않는 최종 소독 희석 단계를 희석배수로 한다.Through this process, the final disinfection dilution step in which two or more growths are not recognized in the nutrient medium of three identical disinfectant dilution drainages is used as the dilution drainage.
상기한 바와 같은 과정을 통하여, 유효희석배수는 처리구 2(유기물 저) 및 처리구 3(유기물 고)은 처리구 4(병원체 대조)를 대조로 하고, 처리구 1(유기물 무)은 처리구 5를 대조로 하여 3번 반복하여 시험한 결과의 중위수(median)로 한다. 이를 구체적으로 설명하기 위해 살모넬라 콜레라스위스(Salmonella cholerasuis)에 대한 살균 효과를 표 4에 나타내었다.Through the process as described above, the effective dilution drainage is treated as treatment 2 (organic low) and treatment 3 (organic high) as treatment 4 (pathogen control), and treatment 1 (organic-free) is treated as treatment 5 The median of the result of the test is repeated three times. To specifically illustrate this, Table 4 shows the bactericidal effect on Salmonella cholerasuis.
즉, 본 발명에 의한 천연 소독제는 살모넬라 콜레라스위스(Salmonella cholerasuis)에 대해서는 유기물 무의 조건에서는 5배, 유기물 저의 조건에서는 10배, 유기물 고의 조건에서는 2배 희석시까지 살균효과가 나타난다.That is, the natural disinfectant according to the present invention exhibits a bactericidal effect until Salmonella cholerasuis is diluted 5 times in the condition of organic matter, 10 times in the condition of organic matter and 2 times in the condition of organic matter.
한편, 본 발명에 의한 천연 소독제의 에드워드균(Edwardsiella tarda)에 대한 살균 효과를 표 5에 나타내었다.On the other hand, Table 5 shows the bactericidal effect against Edward bacteria (Edwardsiella tarda) of the natural disinfectant according to the present invention.
표 5에 나타난 바와 같이, 에드워드균(Edwardsiella tarda)에 대해 본 발명에 의한 천연 소독제는 유기물 무의 조건에서는 13배, 유기물 저의 조건에서는 21배, 유기물 고의 조건에서는 4배 회석시까지 살균효과가 나타난다.As shown in Table 5, the natural disinfectant according to the present invention against Edwardsiella tarda has a bactericidal effect up to 13 times under the condition of organic matter, 21 times under the condition of organic matter and 4 times under the condition of organic matter. appear.
다른 한편, 본 발명에 의한 천연 소독제의 비브리오균(Vibrio anguillarum)에 대한 살균 효과를 표 6에 나타냈다.On the other hand, Table 6 shows the bactericidal effect against Vibrio anguillarum of the natural disinfectant according to the present invention.
따라서, 비브리오균(Vibrio anguillarum)에 대해서는 유기물 무의 조건에서는 170배, 유기물 저의 조건에서는 256배, 유기물 고의 조건에서는 53배 희석시까 지 살균효과가 나타난다.Therefore, for Vibrio bacteria (Vibrio anguillarum), bactericidal effect is achieved until the dilution is 170 times in the condition of organic matter, 256 times in the condition of organic matter and 53 times in the condition of organic matter.
또 다른 한편, 본 발명에 의한 천연 소독제의 화농성 연쇄상구균(Streptococcus iniae)에 대한 살균 효과를 표 7에 나타내었다.On the other hand, Table 7 shows the bactericidal effect of the natural disinfectant according to the present invention purulent Streptococcus iniae.
즉, 화농성 연쇄상구균(Stretococcus iniae)에 대해서는 유기물 무의 조건에서는 32배, 유기물 저의 조건에서는 32배, 유기물 고의 조건에서는 4배 희석시까지 살균효과가 나타난다.In other words, the bactericidal effect of Streptococcus iniae is 32 times in the condition of organic matter, 32 times in the condition of organic matter, and 4 times dilution in the condition of organic matter.
마지막으로, 본 발명에 의한 천연 소독제의 황색 포도상구균(Staphylococcus aureus)에 대한 살균 효과를 표 8에 나타냈다.Finally, the bactericidal effect against Staphylococcus aureus of the natural disinfectant of the present invention is shown in Table 8.
요컨대, 황색 포도상구균(Staphylococcus aureus)에 대해서는 유기물 무의 조건에서는 42배, 유기물 저의 조건에서는 16배, 유기물 고의 조건에서는 2배 희석시까지 살균효과가 나타난다.In other words, for Staphylococcus aureus, the bactericidal effect is shown up to 42 times in the condition of organic matter, 16 times in the condition of organic matter, and 2 times dilution in the condition of organic matter.
이하에서, 본 발명에 의한 천연 소독제를 양어장에서 실제로 사용하는 경우의 유효희석배수에 대해 표 9를 참조하여 설명한다.Hereinafter, the effective dilution drainage when the natural disinfectant according to the present invention is actually used in a fish farm will be described with reference to Table 9.
즉, 살모넬라 콜레라스위스(Salmonella cholerasuis)의 경우, 질병 발생한 지역의 소독조, 출입구, 양어장 바닥, 수조, 차량에 대해서는 2배 희석시까지, 양어공간 및 양어기구에 대해서는 10배 희석시까지, 질병 비발생 지역의 일반차량 및 양어장에 대해서는 2배 희석시까지 소독효과가 나타났다. In other words, Salmonella cholerasuis has a two-fold dilution for disinfection tanks, doorways, fish farm floors, tanks and vehicles, and ten-fold dilution for fish farms and fish utensils. Disinfection effect was shown up to 2 times dilution for local vehicles and fish farms.
또한, 에드워드균(Edwardsiella tarda)의 경우, 질병발생한 지역의 소독조, 출입구, 양어장 바닥, 수조, 차량에 대해서는 4배 희석시까지, 양어공간 및 양어기구에 대해서는 21배 희석시까지, 질병 비발생 지역의 일반차량 및 양어장에 대해서는 4배 희석시까지 소독효과가 나타났다. In the case of Edwardsiella tarda, the disease-prone areas of the disinfection tank, the entrance, the bottom of the fish farm, the tank, and the vehicle are diluted four times, and the fish farming space and fish farming equipment are diluted until 21 times. Disinfection effect was shown up to 4 times dilution for general vehicles and fish farms.
또한, 비브리오균(Vibrio anguillarum)의 경우, 질병 발생한 지역의 소독조, 출입구, 양어장 바닥, 수조, 차량에 대해서는 53배 희석시까지, 양어공간 및 양어기구에 대해서는 256배 희석시까지, 질병 비발생 지역의 일반차량 및 양어장에 대해서는 53배 희석시까지 소독효과가 나타났다. In the case of Vibrio anguillarum, up to 53 times dilution for disinfection tanks, entrances, fish farm floors, water tanks, vehicles, and up to 256 times dilution for fish farming and fish farming areas, Disinfection effect was shown up to 53 times dilution for general vehicles and fish farms.
또한, 화농성 연쇄상구균(Streptococcus iniae)의 경우, 질병 발생한 지역의 소독조, 출입구, 양어장 바닥, 수조, 차량에 대해서는 4배 희석시까지, 양어공간 및 양어기구에 대해서는 32배 희석시까지, 질병 비발생 지역의 일반차량 및 양어장에 대해서는 4배 희석시까지 소독효과가 나타났다. In the case of Streptococcus iniae, up to 4 times dilution for disinfection tanks, entrances, fish farm floors, water tanks, vehicles, and up to 32 times dilution for fish farming and fish farming equipment, and disease-free Disinfection effect was shown up to 4 times dilution for local vehicles and fish farms.
마지막으로, 황색 포도상구균(Staphylococcus aureus)의 경우, 질병 발생한 지역의 소독조, 출입구, 양어장 바닥, 수조, 차량에 대해서는 2배 희석시까지, 양어공간 및 양어기구에 대해서는 16배 희석시까지, 질병 비발생 지역의 일반차량 및 양어장에 대해서는 2배 희석시까지 소독효과가 나타났다. Finally, for Staphylococcus aureus, up to 2 fold dilution for disinfection tanks, entrances, fish farm floors, tanks, vehicles, and 16 fold dilution for fish farming and fish farming in diseased areas. Disinfection effect was shown up to 2 times dilution for general vehicles and fish farms.
이상에서 본 발명에 의한 천연 소독제를 희석 사용하는 것에 대해 상세하게 설명하였다. 이를 요약하여 각 공시세균에 대한 유효 희석 농도를 나타내면 표 10과 같다.The dilution of the natural disinfectant according to the present invention has been described above in detail. In summary, the effective dilution concentrations for each test bacterium are shown in Table 10.
즉, 상기한 바와 같이 살모넬라 콜레라스위스(Salmonella cholerasuis) 외 4종류의 균주에 대하여 경수 및 유기물량이 높은 조건에서 본 발명에 의한 천연 소독제의 소독 효과를 조사한 결과, 살모넬라 콜레라스위스(Salmonella cholerasuis)에 대하여 경수 조건에서는 10배 희석시까지, 유기물량이 높은 조건에서는 2배 희석시까지 소독 효과가 나타났다.That is, as described above, Salmonella cholerasuis (Salmonella cholerasuis) and other four strains were examined for the disinfection effect of the natural disinfectant according to the present invention under conditions of high water and organic matter, Salmonella cholerasuis (Salmonella cholerasuis) In hard water conditions, disinfection effect was shown up to 10-fold dilution and up to 2-fold dilution in high organic matter conditions.
또한, 에드워드균(Edwardsiella tarda)에 대하여 경수 조건에서는 21배 희석시까지, 유기물량이 높은 조건에서는 4배 희석시까지 소독 효과가 나타났으며, 비브리오균(Vibrio anguillarum)에 대하여 경수 조건에서는 256배 희석시까지, 유기물량이 높은 조건에서는 53배 희석시까지 소독 효과가 나타났다.In addition, Edwardsiella tarda showed disinfection effects up to 21-fold dilution in hard water conditions and up to 4-fold dilution in high organic matter conditions, and 256-fold in hard water conditions for Vibrio anguillarum. Until dilution, disinfection effect was shown up to 53 times dilution under high organic matter conditions.
또한, 본 발명에 의한 천연 소독제는 화농성 연쇄상구균(Streptococcus iniae)에 대하여 경수 조건에서는 32배 희석시까지, 유기물량이 높은 조건에서는 4배 희석시까지 소독 효과를 나타냈으며, 황색 포도상구균(Staphylococcus aureus)에 대하여 경수 조건에서는 16배 희석시까지, 유기물량이 높은 조건에서는 2배 희석시까지 소독 효과를 나타냈다.In addition, the natural disinfectant according to the present invention exhibited disinfection effect against P. aureus (Streptococcus iniae) up to 32-fold dilution in hard water conditions and up to 4-fold dilution in high organic matter conditions, and Staphylococcus aureus. ), Disinfection effect was achieved up to 16-fold dilution in hard water conditions and up to 2-fold dilution in high organic matter conditions.
본 발명에 의하면, 양어장용 천연 소독제에 있어서, 적정한 농도로 희석하여 사용시에 질병 예방 및 치료에 효과가 있고, 환경 및 어류에 미치는 영향이 적어 혐오감을 줄일 수 있으며, 나아가, 어민 소득 향상에 기여할 수 있다.According to the present invention, in a natural disinfectant for fish farms, dilution to an appropriate concentration is effective in the prevention and treatment of diseases during use, less impact on the environment and fish can reduce aversion, further contribute to fishermen's income improvement have.
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