KR100462645B1 - Caviar genus Bacillus 03 with floc forming ability and wastewater treatment method using the same - Google Patents
Caviar genus Bacillus 03 with floc forming ability and wastewater treatment method using the same Download PDFInfo
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Abstract
본 발명은 플록 형성능을 갖는 신균주 바실러스 속(Bacillus sp.) KB03(KCTC0482BP)에 관한 것으로서, 이를 이용하여 생물학적으로 폐수를 처리하는 경우 플록 형성능이 우수하여 활성오니 플록내 미생물 군집의 안정화를 촉진시키며 침강성을 개선시켜 화학응집제의 사용비용을 절감시킴으로써 최소의 비용으로 신속히 생물학적 폐수처리의 효율성을 높일 수 있으며, 생물학적 처리시설의 초기 운전시 활성오니의 안정화 기간을 단축하여 운전비용의 절감을 유도할 수 있다.The present invention relates to a new bacterium Bacillus sp. By improving the sedimentation properties and reducing the cost of using chemical coagulants, the efficiency of biological wastewater treatment can be increased quickly and at the minimum cost, and the operating cost can be reduced by shortening the stabilization period of the activated sludge during the initial operation of the biological treatment facility. have.
Description
본 발명은 플록 형성능을 갖는 균주 바실러스 속(Bacillus sp.) KBO3(KCTC 0482BP)에 관한 것으로서, 더욱 상세하게는 신규한 바실러스 속(Bacillus sp.) 미생물을 이용하여 생물학적 폐수처리의 과정 중의 활성오니 플록 내 미생물 군집의 안정화를 촉진시키며 침강성을 개선시켜 적은 비용으로 신속하게 처리의 효율성을 높일 수 있는 방법에 관한 것이다.The present invention relates to a strain Bacillus sp. KBO3 (KCTC 0482BP) having a floc forming ability, and more particularly, active sludge floc during the process of biological wastewater treatment using a novel Bacillus sp. The present invention relates to a method for promoting stabilization and improving sedimentation resistance of microorganisms in a community, thereby increasing treatment efficiency quickly and at low cost.
하수 및 폐수의 처리 방법에는 물리화학적 처리방법과 생물학적 처리방법이 있는 바, 이중 생물학적 처리방법에는 활성오니법, 생물막법, 안정화지법 등 여러 종류가 있지만 특히 활성오니법은 처리효율이 좋기 때문에 가장 많이 이용되고 있다.Sewage and wastewater treatment methods include physical and chemical treatment methods and biological treatment methods. Among them, there are various types of biological treatment methods, such as activated sludge method, biofilm method, and stabilization paper method. It is used.
활성오니법에 의한 생물학적 처리는 기본적으로 미생물의 대사작용 즉, 미생물 고유의 기능에 의한 폐수 내에 존재하는 유기물의 분해, 합성, 변환기능을 이용하여 경제적으로 폐수를 처리하는 방법으로서, 주로 폐수 중의 유기물 제거, 질소, 인의 제거, 발생 슬러지의 감량화, 자원 회수 및 처리의 효율화를 목표로 하여 이용되고 있다.Biological treatment by the activated sludge method is basically a method of treating wastewater economically by using the metabolism of microorganisms, that is, decomposition, synthesis, and conversion of organic matter present in wastewater by inherent functions of microorganisms. It is used for the purpose of removal, removal of nitrogen and phosphorus, reduction of generated sludge, resource recovery and treatment efficiency.
그러나, 이와같은 활성오니 방법도 여러 가지 단점을 가지고 있다.However, such activated sludge methods also have a number of disadvantages.
이와같은 점을 해결하기 위한 일환으로 대한민국 특허 출원 제91-25833호에서는 폐수중의 단백질 또는 펩톤에 대한 탄수화물의 중량비를 조절하여 처리하여 활성오니 플록 중의 고분자 물질의 축적을 억제하는 생물학적 폐수처리 방법에 대하여 개시하고 있다.In order to solve this problem, Korean Patent Application No. 91-25833 discloses a method for treating biological wastewater that controls the accumulation of high molecular weight substances in activated sludge by controlling the weight ratio of carbohydrates to protein or peptone in the wastewater. It is disclosed.
그리고, 미생물 성장억제제로 안식향산류를 사용한 폐수처리용 미생물제제(특허출원 제91-5155호)에 대해서도 개시되어 있고, 그밖에도 생물학적 방법으로 폐수를 처리하는 방법에 대하여 다양한 방법이 개시되어 있다(특허출원 제94-29086호, 제94-25926호).In addition, a microbial agent for wastewater treatment using benzoic acid as a microbial growth inhibitor (patent application No. 91-5155) is disclosed, and various methods for treating wastewater by a biological method are disclosed. Application 94-29086, 94-25926).
그러나, 상기와 같은 방법은 대부분 공정개선이나 미생물의 유기물 제거측면만 중점적으로 연구하고 있다.However, most of the above methods focus on improving the process or removing organic matter from microorganisms.
통상 활성오니를 이용한 생물처리에 있어서 나타나는 문제점은 사상성 세균에 의한 벌킹현상과 고부하 또는 빈부하시 발생되는 핀플록 현상, 또는 오니일령의 증가에 의한 오니해체 현상 등이 대표적이다.In general, problems in biological treatment using activated sludge are typical of the bulking phenomenon caused by filamentous bacteria, pin floc phenomenon occurring at high or poor load, or sludge disintegration due to an increase in sludge age.
이를 해소하기 위해서는 일반적으로 화학응집제를 폭기조에 투입함으로써 일시적인 효과를 볼 수 있으나, 궁극적인 해결방안으로 볼 수 없으며 오히려 미생물의 폐수처리 기능을 상실하게 하는 역할로 나타나고 있다.In order to solve this, chemical coagulant is generally added to the aeration tank to provide a temporary effect, but it cannot be seen as an ultimate solution, but rather appears to play a role in losing the microbial wastewater treatment function.
뿐만 아니라, 화학응집제를 투입함에 따라 추가적으로 비용이 발생되는 문제점도 있다.In addition, there is a problem that additional costs are generated as a chemical coagulant is added.
이에 본 발명자들은 미생물의 폐수처리 기능을 유지하면서도 지속적이며 고효율의 플록 형성기능을 갖는 미생물을 통해 활성오니 플록 내 미생물 군집의 안정화를 촉진시키며 침강성을 개선시켜 화학응집제의 사용비용을 절감시키고 최소의 비용으로 신속히 생물학적 폐수처리의 효율성을 높일 수 있는 균주를 찾아내고자 노력하였다.Therefore, the present inventors promote the stabilization of the microbial community in the active sludge and improve the sedimentation through the microorganisms having the continuous and highly efficient floc formation function while maintaining the wastewater treatment function of the microorganisms, thereby reducing the use cost of the chemical coagulant and the minimum cost. In an effort to quickly find a strain that can increase the efficiency of biological wastewater treatment.
본 발명의 목적은 플록 형성능이 우수하여 활성오니 플록내 미생물 군집의 안정화를 촉진시키며, 침강성을 개선시켜 화학응집제의 사용비용을 절감시키고 최소의 비용으로 신속히 생물학적 폐수처리의 효율성을 높일 수 있을 뿐만 아니라, 생물학적 처리시설의 초기 운전시 활성오니의 안정화 기간을 단축하여 운전비용의 절감을 유도할 수 있는 신균주를 제공하는 데 있다.An object of the present invention is to promote the stabilization of microbial community in the active sludge with excellent floc forming ability, to improve the sedimentation can reduce the use cost of chemical flocculant and to quickly increase the efficiency of biological wastewater treatment at a minimum cost Therefore, the present invention provides a new strain capable of reducing the operating cost by shortening the stabilization period of the activated sludge during the initial operation of the biological treatment facility.
본 발명의 다른 목적은 이와같은 신균주를 이용하여 생물학적 방법으로 폐수를 처리하는 방법을 제공하는 데 있다.Another object of the present invention is to provide a method for treating wastewater by a biological method using such a new strain.
이와같은 목적을 달성하기 위한 본 발명의 신균주는 플록 형성능을 갖는 바실러스 속(Bacillus sp.) KB03(KCTC 0482BP)이다.The new strain of the present invention for achieving this object is Bacillus sp. KB03 (KCTC 0482BP) having a floc forming ability.
이와같은 본 발명을 더욱 상세하게 설명하면 다음과 같다.The present invention will be described in more detail as follows.
본 발명은 플록 형성능을 갖는 신균주 바실러스 속 KB03에 관한 것으로서, 이 균주의 분리, 동정은 다음과 같다.The present invention relates to a new strain Bacillus genus KB03 having a floc forming ability, the isolation and identification of this strain is as follows.
신균주의 분리Isolation
산업폐수로부터 시료를 채취하여 이 시료를 멸균한 식염수에 희석한 후 포테이토 덱스트로스 아가 배지에 도말하여 20∼30℃에서 2일간 배양하여 균주를 분리하였다. 분리된 균주를 각각 뉴트리언트 브로스 배지를 사용하여 20∼30℃에서 2일간 배양한 후 그 배양액으로 플록형성능 시험을 하여 플록형성능을 갖는 미생물을 선정한다.Samples were collected from industrial wastewater, and the samples were diluted in sterile saline solution, smeared in potato dextrose agar medium, and incubated at 20 to 30 ° C for 2 days to isolate strains. The isolated strains were incubated at 20 to 30 ° C. for 2 days using nutrient broth medium, and then the floc forming ability test was performed with the culture medium to select the microorganisms having the floc forming ability.
신균주의 동정Sympathy of New Mycology
상기에서 분리된 균주를 버기스 매뉴얼에 따라 동정하였다. 이때, 동정을 위해 시험된 항목과 그 결과는 다음 표 1∼3과 같다.Strains isolated from above were identified according to the Bugis manual. At this time, the items tested for identification and the results are shown in Tables 1 to 3 below.
여기서, 표 1은 형태적 특성을 나타낸 것이고, 표 2는 생리생화학적 특성을 나타낸 것이며, 표 3은 지방산 조성을 측정한 것이다.Here, Table 1 shows the morphological properties, Table 2 shows the physiological biochemical properties, Table 3 is a measurement of fatty acid composition.
상기 표 1, 2 및 3의 결과에 따라 본 발명에서 분리해 낸 균주는 바실러스속(Bacillus sp.)에 속하는 것으로 판명되었다. 이 균주를 바실러스속 KB03이라 명명하고 한국과학기술연구원 부설 생명공학연구소 내 유전자 은행에 1998년 5월 26일자로 기탁하였고, 수탁번호 KCTC 0482BP를 부여받았다.According to the results of Tables 1, 2 and 3, the strain isolated in the present invention was found to belong to the genus Bacillus ( Bacillus sp.). The strain was named KB03 genus and was deposited with the Gene Bank within the Biotechnology Research Institute of the Korea Institute of Science and Technology on May 26, 1998 and was given accession number KCTC 0482BP.
이하, 본 발명을 실시예에 의거 상세히 설명하면 다음과 같은 바, 본 발명이 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail with reference to Examples, but the present invention is not limited by the Examples.
실시예 1Example 1
바실러스 속 KB03 균주를 글루코오즈 3%, NaNO3 0.1%, K2HPO4 0.1%, KH2PO4 0.08%, MnSO4·4H2O 0.01% 및 NaCl 0.05%가 포함된 배양액에서 32℃, pH 6.5의 조건으로 배양하여 균주의 배양액을 얻었다.The Bacillus KB03 strain glucose 3%, NaNO 3 0.1%, K 2 HPO 4 0.1%, KH 2 PO 4 0.08%, MnSO 4 · 4H 2 O 0.01% and 32 ℃ in a culture medium that contains NaCl 0.05%, pH Cultured under the conditions of 6.5 to obtain a culture of the strain.
실시예 2Example 2
바실러스 속 KB03 균주의 무기성 입자와 유기성 입자에 대한 응집침강 효율을 알아보기 위하여 다음과 같이 실시하였다.In order to determine the coagulation sedimentation efficiency of the inorganic particles and the organic particles of the KB03 genus strain was carried out as follows.
무기성 입자로 카오린(준세이 공업, 일본) 5000mg/ℓ을 증류수에 현탁시켜 급속 교반하고 상기 실시예 1로부터 얻은 본 발명 균주의 배양액을 첨가한 후 완속교반하여 플록의 침강성을 측정하였고, 유기성 입자로 하수처리장 폭기조 활성오니를 초음파 분쇄기와 마쇄기로 분쇄한 후 현탁성 입자만을 회수하여 2000mg/ℓ로 조정한 후 무기성 입자와 같이 플록의 침강성을 측정하였다. 침강성의 측정은 플록이 형성되어 침강할 때 수면에서 슬러지 부피가 50%에 도달되는 시점을 침강속도로 환산하여 나타내었다.Suspension of floc was measured by suspending 5000 mg / l of kaolin (Junsei Kogyo, Japan) in distilled water as an inorganic particle and rapidly stirring and adding the culture solution of the strain of the present invention obtained in Example 1, and then stirring it slowly to determine the flocculation. After the aeration tank activated sludge in the sewage treatment plant was pulverized by an ultrasonic grinder and a crusher, only suspended particles were collected and adjusted to 2000 mg / l, and then the floc was settled like the inorganic particles. The sedimentation measurement was expressed in terms of sedimentation rate when the floc formed and settled to 50% of the sludge volume on the surface.
그 결과는 다음 표 4와 같다.The results are shown in Table 4 below.
상기 표 4의 결과로부터 본 발명의 균주 배양액을 처리한 것은 대조구에 배하여 무기성 입자의 경우 6.5배, 유기성 입장의 경우 5배 정도 향상된 침강속도를 나타냄을 알 수 있다.From the results of Table 4, the treated strain culture solution of the present invention can be seen that the sedimentation rate improved by 6.5 times for the inorganic particles, 5 times for the organic position in the control.
실시예 3Example 3
본 발명 균주를 이용하여 실제 폐수에 적용하였을 때의 효과를 알아보기 위하여 다음과 같이 실시하였다.In order to determine the effect when applied to the actual wastewater using the strain of the present invention was carried out as follows.
대상폐수는 도축폐수로 고부하에 의한 플록의 분산이 나타나 30분간의 침강슬러지가 90% 이상으로 나타났고, 결과적으로 방류수의 수질이 기준치 이상으로 악화되었다. 대상폐수를 실험실 내에 운반하여 2ℓ 용량의 시험폭기조를 이용하여 현장의 처리조건과 동일하게 운전한 다음, 화학적 산소요구량(COD), 생물화학적 산소요구량(BOD), 현탁고형물(SS), 침강슬러지(SV30) 및 슬러지용적지수(SVI)를 측정하였으며 그 결과를 다음 표 5에 나타내었다. 이때, 처리구는 상기 실시예 1에 따라 얻어진 본 발명 균주의 배양액을 운전개시일에 200mg/ℓ투여하고, 이후로 20mg/ℓ을 매일 투여한 것으로 하였다. 실험검정은 운전개시일 후 5일차에 방류수를 대상으로 실시하였다.The target wastewater was slaughtered wastewater, which showed floc dispersion due to high load, resulting in more than 90% of sedimentation sludge over 30 minutes. The wastewater was transported to the laboratory and operated in the same conditions as the on-site treatment conditions using a 2 liter test aeration tank. SV30) and sludge volume index (SVI) were measured and the results are shown in Table 5 below. At this time, the treatment was administered 200mg / ℓ of the culture medium of the strain of the present invention obtained according to Example 1 at the start of operation, and then 20mg / ℓ was administered daily. Experimental tests were performed on the discharged water on the 5th day after the start of operation.
상기 표 5의 결과로부터 처리구는 대조구에 비하여 30분간의 침강슬러지(SV30)와 슬러지용적지수(SVI)가 크게 감소하였으며, 이와 더불어 화학적 산소요구량(CODMn), 생물화학적 산소요구량(BOD), 현탁고형물(SS) 역시 감소하는 것을 알 수 있다.From the results of Table 5, the treatment group had significantly reduced sedimentation sludge (SV30) and sludge volume index (SVI) for 30 minutes, as well as chemical oxygen demand (COD Mn ), biochemical oxygen demand (BOD), and suspension compared to the control. Solids (SS) can also be seen to decrease.
이상에서 상세히 설명한 바와 같이, 본 발명에 따른 신균주 바실러스 속 KB03(KCTC 0482BP)는 플록 형성능이 우수하여 생물학적 폐수처리의 과정 중의 활성오니 플록 내 미생물 군집의 안정화를 촉진시킬 수 있고, 침강성을 개선시켜 적은 비용으로 신속하게 폐수처리의 효율성을 높일 수 있는 효과가 있다. As described in detail above, the new strain Bacillus genus KB03 (KCTC 0482BP) according to the present invention has excellent floc forming ability to promote the stabilization of the microbial community in the activated sludge floc during biological wastewater treatment, There is an effect that can quickly increase the efficiency of wastewater treatment at a low cost.
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