KR100489614B1 - Pseudomonas sp. T-1 having a ability to resolve high-density Terephthalic acid and a method of waste-water treatment using the same - Google Patents

Abstract

The present invention is a novel microorganism Pseudomonas sp . T-1, which is good in aerobic and breathable conditions and has excellent resolution of terephthalic acid, and is discharged by a polyester weight loss process using the same. A method for biologically treating synthetic wastewater containing terephthalic acid.

Pseudomonas T-1 (Pseudomonas sp. T -1) is a nitrogen atom, personnel, the inorganic salts as base and as the selected microorganism in a terephthalic acid containing as a carbon source medium, I inhibition of microbial growth by incorporation of a decomposable material terephthalic acid is No impact load phenomenon due to flow rate and BOD load fluctuations, so it is an excellent new microorganism capable of completely decomposing terephthalic acid contained in industrial wastewater discharged from polyester weight loss process.

In addition, the biological treatment process of the wastewater containing terephthalic acid of the present invention, the treatment of wastewater containing a terephthalic acid, neutralizing the raw water, aeration of the neutralized wastewater in the aeration tank, precipitation of the wastewater that passed through the aeration tank And the step of pressurizing the wastewater that has passed through the settling tank, precipitating the wastewater that has passed the pressurized flotation step, and discharging the purified separated water.

Description

Terephthalic acid (Ｔｅｒｅｐｈｔｈａｌｉｃａｃｉｄ) Microbial Pseudomonas tee-1 (PES-T-1) having excellent degradability and a method for treating wastewater containing terephthalic acid using the same {Pseudomonas sp. T-1 having a ability to resolve high-density Terephthalic acid and a method of waste-water treatment using the same}

The present invention relates to a novel microorganism Pseudomonas sp . T-1 having a terephthalic acid (TPA) decomposing ability and a method for treating wastewater containing terephthalic acid using the same. More specifically, Pseudomonas sp . 1 ( Pseudomonas sp . T), a microorganism having good growth under aerobic and breathable conditions, resistant to changes in pH, temperature, flow rate and biochemical oxygen demand (BOD), and having excellent resolution of terephthalic acid. -1) and a method for biologically treating wastewater containing terephthalic acid using the same.

The current load and treatment status of dyeing wastewater by industry is in the case of dyeing complex where wastewater discharged from individual dyeing processing companies is collected and treated in a joint storage tank, and the chemical oxygen demand (COD) load by waste industry is reduced by polyester. 68% for processing, 22% for cotton, and 11% for other biochemical oxygen demand (BOD) loads (84% for polyester reduction, 11% for cotton, and 5% for other cotton). Due to the large amount of wastewater contained.

The number of companies involved in dyeing is about 5.02% of the total, but the wastewater discharge is about 316,000 tons / day, accounting for 16.61% of the total emissions, making it the second largest proportion of the total wastewater industry. In particular, since the wastewater contains a large amount of hardly decomposable substances such as terephthalic acid in the wastewater, the treatment efficiency is low even if the existing treatment method using the flocculation process and the activated sludge process is used. Terephthalic acid is a representative cause of environmental pollution. It is known to have a very big impact on public waters as a substance.

Conventionally, a method of treating waste by treating polyester wastewater with sulfuric acid, neutralizing it to pH 4 to precipitate and precipitate terephthalic acid, and then dehydrating and drying it, but the precipitated terephthalic acid sludge contains 88% water. Therefore, it has been pointed out that there are many problems in the treatment of the dehydrated liquid which is a high concentration of wastewater.

Chemical treatment of hardly degradable substances or harmful chemicals has been a difficult task, but recently, it has been considered as a technology that can be sufficiently solved by applying biotechnology, and various researches on biotechnological treatment methods have been conducted at home and abroad.

Benzene digestion method (Eric et al., Appl. Environ. Microbiol., Vol. 55, p.3221, 1989), Pseudomonas putida and Kreptococosellinobi were selected as screening and using strains having the ability to degrade specific aromatic compounds. Method of decomposing phenol by mixed culture and immobilization on activated carbon (Rehm et al., Appl. Microbiol. Biotechnol. Vol. 16, p283. 1987) and naphthalene decomposition method using Pseudomonas genus (Kobayshi et al., Environ. Sci. Technol., Vol. 16, p170, 1982) have been conducted in various angles to apply the microorganisms to the wastewater treatment of industrial sites.

However, only a limited number of hardly decomposable microorganisms have been developed, and the scope thereof has been limited. Researches on microorganisms that decompose terephthalic acid have not been conducted. Due to the lack of stability, it was urgent to develop microorganisms that were found to have excellent resolution in actual wastewater treatment.

Therefore, it is necessary to select microorganisms that decompose terephthalic acid and apply them to the treatment of polyester weight loss wastewater.In order to select and isolate microorganisms that decompose such substances, it is important to select a source and select a target substance. have.

Korean Patent No. 142,106 proposes Pseudomonas TS2, a novel microorganism using terephthalic acid as a substrate, and a method of treating wastewater using the same. However, this microorganism had a problem in that it could not completely decompose terephthalic acid.

The present inventors have conducted studies to solve the above problems, microorganisms that can decompose high-load, multimodal and malignant industrial wastewater containing terephthalic acid, a biodegradable material discharged from the polyester weight loss process Focusing on the point of view, samples were collected from wastewater and pollutants discharged from polyester weight reduction process of various textile factories in Korea for many years to separate bacteria which can decompose terephthalic acid component, and we conducted decomposition experiment of wastewater of terephthalic acid. Pseudomonas sp . T-1 was identified as a species with excellent resolution.

The present invention provides a microorganism having good growth under aerobic and breathable conditions and having excellent resolution of terephthalic acid which completely decomposes terephthalic acid even in actual weight loss wastewater treatment, and an effective method for biologically treating terephthalic acid-containing industrial wastewater using the microorganism. Its purpose is to.

In addition, the present invention does not suppress the growth of microorganisms due to the incorporation of hardly decomposable substances such as terephthalic acid, and the impact load phenomenon due to fluctuations in the flow rate and BOD load does not appear, and thus, a process for lowering the load by pre-aggregation treatment is unnecessary. It is an object of the present invention to provide a method for treating waste pulverized using the novel microorganism of the present invention.

The present invention is a novel microorganism Pseudomonas sp . T-1, which is a good microorganism having good growth under aerobic and breathable conditions, and has excellent resolution of terephthalic acid, and a method for treating wastewater containing terephthalic acid using the same. It is about.

More specifically, Pseudomonas sp.T ( Pseudomonas sp . T), which is a microorganism having good growth under aerobic and breathable conditions, resistant to changes in pH, temperature, flow rate and biochemical oxygen demand (BOD), and having excellent resolution of terephthalic acid. -1). The present invention also relates to a method for biologically treating wastewater containing terephthalic acid using the microorganism.

The present inventors collected the soil (pollution source) of the rivers surrounding the textile factory in Daegu area. The novel microorganism of the present invention is based on a nitrogen source, a person, an inorganic salt, and using a minimal medium containing terephthalic acid as the only carbon source, after separating the bacteria that can use terephthalic acid as a carbon source, it is selected as the strain having the highest resolution of terephthalic acid. It is. The characteristics of the novel microorganism according to the present invention are as follows. In more detail, the minimum medium is based on ammonium sulfate, potassium dihydrogen phosphate, sodium chloride and magnesium sulfate, and contains terephthalic acid as the only carbon source.

The results of examining the morphological and physiological characteristics of the isolates are shown in Tables 1 and 2 below. The isolates were Gram negative and motility as bacilli. In addition, as a result of examining the cultural properties, the catalase test, gelatin liquefaction ability, and nitrate reduction ability were all positive. Urease test, hydrogen sulfide production capacity (H ₂ S production) was negative. Carbohydrate availability includes glucose, mannose, maltose, maltose, sucrose, mannitol, citrate, gluconate, and N-acetylglucosamine And other carbohydrates were not available or weak.

Therefore, Pseudomonas genus was identified according to Bergeys Manual of determinative bactoeriology (10th ed.) And Manual for the Identification of medical bacteria (2nd ed.) According to the morphological, cultural and physiological characteristics of the isolated strains. Was identified.

Accordingly, the novel microorganism of the present invention was named Pseudomonas sp . T-1, and was deposited on July 20, 2002 under the accession number KFCC-11309 to the Korea Microbial Conservation Center, which is affiliated with the Korean spawn association.

Table 1. Morphological and Cultural Characteristics of Bacteria

Gram staining  voice(-)  Form  Rod Motility Motile  Optimal temperature 30 ℃ Optimum pH (Opt. PH) 7.5 Colony color Brown

Table 2. Physiological Characteristics and Carbon Source Availability of Bacteria

 Glucose + Arabinose ± Mannoose + Glucose + Glucose + Rhamnose-Melibiose ± Maltose + Sucrose + Mannitol + Mannitol + Sorbitol-Inositol-Gluconate + Citrate + Phenylacetate-N-acetylglutamsamine + N-acetylglucosamine Oxidase test- Indole production-Gelatin liquefaction + VP test-Nitrate reduction + Urease test-Hydrogen sulfide production (H ₂ S production)-OF test 0 Lysinedihydrola Ase-(Lysine dehydrolase) Ornithine dioxylase-(Ornitine decarboxylase) Arginine dihydrolase + (Arginine dehydrolase) Beta-galactosidase + Catalase test + Fluorescent pigment pigments) + Starch hydrolysis +

  Positive; - voice

Polyester wastewater is not only biologically decomposable but also hardly decomposable. However, existing treatment plants are pretreated by flocculation and then treated by activated sludge, resulting in low dissolved oxygen in aeration tanks and sludge bulking. There is a lot of difficulty in comprehensively treating wastewater such as generation. In the existing treatment plant, the first thing to coagulate is because the BOD load in the aeration tank is not high and waste water treatment is not performed. Therefore, the coagulation treatment is performed to pretreat the coagulation process to lower the BOD load.

In addition, since the composition of the dyeing wastewater is complex composition and large water quality fluctuation, various treatment methods and combinations thereof have been examined, but the exact wastewater treatment system has not been established. Therefore, in the existing treatment plants, condensation treatment is performed as a pretreatment to reduce the load of biochemical oxygen demand as the efficiency of wastewater treatment decreases due to the impact load caused by the flow rate and the load of biochemical oxygen demand (BOD) and the bulking phenomenon occurs. There is a situation.

However, the bacteria isolated in the present invention is resistant to changes in pH and temperature, there is no suppression of the growth of microorganisms due to the incorporation of hardly decomposable substances, and there is no impact load phenomenon due to load fluctuations in flow rate and biochemical oxygen demand. The treatment efficiency was also increased by the same process as 4 (raw water-neutralization-aeration tank-precipitation tank-pressurization tank-precipitation tank - discharge).

The process of treating wastewater containing terephthalic acid using Pseudomonas T-1 includes neutralizing raw water, aeration of the neutralized wastewater in an aeration tank, precipitating wastewater passing through the aeration tank, and passing through the precipitation tank. Pressurizing one of the wastewater; and again precipitating the wastewater that has passed through the pressurization step, and discharging the purified separated water. It will be described in more detail below step by step.

The raw water from the dyeing process, which is the raw water, is strong alkaline wastewater with a pH of about 12. Therefore, for biological treatment, the optimum pH of the bacterium should be formulated. Since the optimum pH of the bacterium was found to be 6 to 8, in order to adjust the optimum pH of the bacterium, an acid such as sulfuric acid was added to raw water to adjust the pH. Neutralization process to adjust to 6-8 is necessary.

In the aeration tank stage, the microorganism of the present invention is cultured and introduced into the aeration tank, thereby decomposing the hardly decomposable substance.

In the sedimentation tank stage, the microorganisms in the aeration tank are separated from the treated water, and the concentration of the microorganisms is controlled.

In the pressure flotation tank step, the organic matter remaining in the supernatant of the precipitation tank is completely removed from the pressure flotation tank, the wastewater passing through the pressure flotation tank is precipitated again, and finally, the separated water purified by the process is discharged. do.

The following examples further illustrate the invention, but are not intended to limit the scope of the invention to the following examples.

Example 1 Screening of Terephthalic Acid Degrading Strains

To screen for microorganisms with excellent terephthalic acid degradability and growth, samples were taken from wastewater treatment plants and nearby streams in the dyeing complex of Daegu, and then suspended from the sample with sterile physiological saline or buffer solution, and then containing flat terephthalic acid. Plated in a minimal medium and incubated at 30 ℃. At this time, the minimum medium used was 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.05% sodium chloride, 0.05% magnesium sulfate, and 1.5-3% agar, and each medium was added with the required amount of terephthalic acid. do.

Colonies obtained through the culture are secured through a screening operation through continuous repeat culture while rotating shaking in a minimal medium containing 1% terephthalic acid to secure a large number of terephthalic acid degradation strains.

They are grown in a terephthalic acid-containing minimum medium in which the concentration of terephthalic acid is changed from 1 to 5% in 1% increments, so that growth is not delayed even at high concentrations of terephthalic acid, and it is possible to directly and quickly complete the high concentration of terephthalic acid in wastewater. Passage yields a single, pure colony.

At this time, in order to measure the terephthalic acid and to determine the resolution of the terephthalic acid of the test bacteria, a small amount of bacteria were inoculated by UV scanning with UV / Vis (UV / Vis) and over time using a high pressure liquid chromatography column (HPLC). The degradation degree of terefe deoxidation was investigated (FIG. 2).

(Example 2)

In order to treat the wastewater containing terephthalic acid using the microorganism of Example 1 was subjected to the following treatment.

The raw water, which is the wastewater of the dyeing plant, is a strong alkaline wastewater with a pH of about 12. Therefore, for biological wastewater treatment, the optimal pH condition of the bacterium should be adjusted. The optimum pH of the bacterium was found to be between 6 and 8. In order to adjust the pH by adjusting the acid such as sulfuric acid was adjusted between 6-8.

The effective capacity of the aeration tank is 13.8 L and it is made of acrylic resin having a thickness of 5 mm. In order to maintain the temperature of the reactor, the temperature in the reactor was maintained at 30 ± 2 ℃ using a water jacket.

In order to supply the raw water and the sludge return to the reactor, the metering pump was continuously supplied, and the dissolved oxygen concentration of the reactor was maintained at 1.0 to 2.0 mg / l using an air blower. In addition, in order to maintain the CNP balance of the influent, nutrients are injected so that the chemical oxygen demand (COD): nitrogen (N): phosphorus (P) is maintained at 100: 5: 1 by using urea and phosphoric acid. The hydraulic residence time of the reactor was maintained at 30 to 35 hours.

Then, in the aeration tank, Pseudomonas ti-1 of the present invention was cultured and added to the aeration tank, and in the precipitation tank, the microorganism and the treated water in the aeration tank were separated and the concentration of the microorganism was adjusted. The organic matter remaining in the supernatant of the settling tank is completely removed from the pressurized floatation tank, the wastewater passing through the pressurized floatation tank is settled again, and finally, the discharged water (separated water) is discharged to complete the treatment of the wastewater.

As a result, the sludge returned from the 25th operation, and the aeration tank residence time was reduced from 35 hours to 30 hours, but the COD of 20.1-33.5 mg / ℓ and BOD of 0.4-3.5 mg / ℓ had no significant change in the water quality. The treated water was stably obtained. In addition, terephthalic acid was 4-9% in batch type and 2-3% in continuous type, but it was not detected at all in aeration tank and effluent by treatment with this bacteria, so terephthalic acid was 100 % Was shown to be removed (Table 3).

Table 3. Results of the application of this microorganism to the wastewater of the dyeing complex (water quality of effluent)

Item Effluent COD _Mn 20.1 to 33.5 mg / l COD _Cr 35.1 to 49.2 mg / l BOD ₅ 0.4 to 3.5 mg / l Total Organic Carbon (TOC) 21.4-31.73 mg / l Terephthalic acid Not detected Suspended solids (SS) 5-20 mg / l pH 7.5 to 8.2

Experimental Example 1

In order to check the resolution of terephthalic acid of Pseudomonas sp. T-1, the growth of bacteria and the concentration of terephthalic acid over time were cultivated while culturing the strain in a liquid medium in which the initial concentration of terephthalic acid was adjusted to 1000 ppm. As a result, after 36 hours the residual amount of terephthalic acid was 100ppm, the decomposition rate was 90%, the residual amount after 60 hours incubation was 20ppm, terephthalic acid was almost completely decomposed after 72 hours (Fig. 1). Therefore, it was shown that the application of Pseudomonas-1 to the wastewater treatment can completely decompose the terephthalic acid contained in the wastewater.

Experimental Example 2

In order to measure the resolution of terephthalic acid, the content of terephthalic acid in the medium was measured by terephthalic acid using high pressure liquid chromatography (HPLC columns) while incubating Pseudomonas sp -1 in a medium containing terephthalic acid. As a result of measuring the content of the terephthalic acid was shown to decompose over time and completely removed as shown in FIG.

Experimental Example 3

In order to investigate the effect of terephthalic acid on the growth of Pseudomonas-1, the concentration of terephthalic acid was varied in stages from 0.05% to 8%, and the effect of terephthalic acid on the growth of Pseudomonas-1 was investigated. , Up to 5% had no effect on the growth of the bacteria, but at 5% or more, the growth of the bacteria was inhibited. Thus, when the wastewater was treated by the bacterium, the concentration of TPA was up to 5% (Fig. 3).

As can be seen from the above description, the novel Pseudomonas sp . T-1 of the present invention is a microorganism selected from a medium containing a nitrogen source, a person, an inorganic salt, and containing terephthalic acid as a carbon source. There is no suppression of microbial growth by mixing terephthalic acid, which is a hard-decomposable substance, and no impact load due to fluctuations in flow rate and BOD load. Seems.

In addition, the present invention, by treating the dyeing wastewater by microorganisms, mineralizes specific hardly decomposable organic compounds contained in the dyeing wastewater to be used as a carbon source or energy source for growth, or chemical structure, even if it does not magnetize toxic contaminants It is effective in contributing to environmental cleanup by changing to non-toxic substances.

FIG. 1 shows the growth of bacteria and degradation of terephthalic acid over time while culturing Pseudomonas sp. T-1 in a minimal medium containing terephthalic acid.

FIG. 2 shows a process in which terephthalic acid is decomposed over time using a high pressure liquid chromatography (HPLC) while culturing Pseudomonas ti-1 in a minimal medium containing terephthalic acid.

Figure 3 shows the effect of the concentration of terephthalic acid on the growth of Pseudomonas ti-1 while changing the concentration of terephthalic acid in stages.

Figure 4 shows a comparison of the conventional wastewater treatment process and wastewater treatment process using Pseudomonas ti-1 of the present invention.

Claims (4)

delete Microorganism Pseudomonas sp. T-1, which is selected as a microorganism having a decomposing ability of terephthalic acid in a minimum medium containing terephthalic acid as the sole carbon source based on nitrogen sources, personnel and inorganic salts. (KFCC-11309). A method for treating wastewater containing terephthalic acid using the microorganism Pseudomonas sp. T-1 (KFCC-11309) of claim 2. Neutralizing the raw water containing terephthalic acid, aeration of Pseudomonas sp. T-1 (KFCC-11309) according to claim 2 together with the neutralized wastewater in the aeration tank, and the wastewater passing through the aeration tank Precipitating the wastewater that has passed through the settling tank, precipitating the wastewater that has passed through the pressurizing flotation step, and discharging the purified separation water to discharge the purified sewage water. .