KR20040087383A - Composition and method for treating contaminated water by using Paenibacillus kribbensis AP-2 - Google Patents

Abstract

PURPOSE: A water treatment composition is provided which is more environmentally friendly and safer to the human body and has superior control capacity of environmental factors compared with an existing water treatment composition using chemical synthetic materials, and a water treatment method using the same is provided. CONSTITUTION: The composition comprises a viscous substance produced by Paenibacillus kribbensis AP-2 (KCTC 10421BP), wherein the composition further comprises one or more materials selected from alum, polyaluminum chloride (PAC) and polyaluminum chloride silica (PACS). The composition is used to remove heavy metal ions contained in water system to be treated, and wherein the heavy metal ions are aluminum ions (Al¬+3) or copper ions (Cu¬+2).

Description

Composition and method for water treatment using penibacillus cribensis api-2 strain {Composition and method for treating contaminated water by using Paenibacillus kribbensis AP-2}

The present invention relates to a novel environmentally friendly composition for water treatment and a water treatment method using the same, and more particularly, Paenibacillus kribbensis AP-2; accession number-KCTC10421BP, deposit date-2003 May 10) relates to an environment-friendly water treatment composition using a viscous material produced by the strain and a water treatment method using the same.

In order to supply clean water resources that are most needed for daily life, it is necessary to remove suspended solids (SS) and various organic and inorganic materials that cause turbidity in the water system in particular. Accordingly, for the removal of the solid suspended substances contained in various water systems (including purified water, sewage and wastewater, etc.), most of them are manufactured by chemical synthesis such as polyacrylamide (PAA, polyacrylamide), aluminum sulfate (Alum), and the like. The compounds to be used are mainly used for the treatment of aqueous systems. In particular, for water treatment, alum, polyaluminum chloride (PAC) or polyaluminum chloride silica (PACS) is mainly used.

However, as the above chemical compounds are continuously used for a long time as a water treatment agent, the problem caused by the accumulation of aluminum, which is a heavy metal in the case of alum, and a strong neurotoxin depending on the degree of use in the case of polyacrylamide (PAA) There are many concerns about the environmental and safety aspects of many chemical synthetic materials that are currently widely used in water treatment formulations such as wastewater and sewage treatment, such as the problem of being able to work. {Dearfield & Ambermathy, 1988, "Acrylamide: its metabolism, developmental and reproductive effects, genotoxicity, and carcinogenicity," Mutant Research. 195; 45-77}.

Therefore, there is a great need for the development of water treatment formulations and water treatment methods, which are more environmentally friendly and safe for the human body, and have excellent control of environmental factors (including coagulation activity and turbidity, total nitrogen removal, chemical oxygen demand, and heavy metal removal). It is becoming.

The object of the present invention is more environmentally friendly and safer to the human body than the conventional water treatment formulations and water treatment methods using chemical synthetic materials, and environmental factors (coagulation activity and turbidity, total nitrogen removal ability, chemical oxygen demand, heavy metal removal, etc.). It is to provide a composition for water treatment and a water treatment method using the same having better controllability.

FIG. 1 is a scanning electron micrograph (× 8,000) of a cultured Paenibacillus kribbensis AP-2 (Accession No.—KCTC10421BP) strain used in the present invention.

Figure 2 is a graph of HPLC analysis of the components of the viscous substance obtained through the culture of the strain of Paenibacillus kribbensis AP-2 (Accession No.-KCTC10421BP) used in the present invention.

Figure 3 is an infrared spectrogram graph of the main functional groups of the viscous material obtained through the cultivation of the strain of Paenibacillus kribbensis AP-2 (Accession No.-KCTC10421BP) strain used in the present invention .

Figure 4 is a graph showing the change of the final pH, strain growth, viscosity and coagulation activity with the culture time of the culture of the Paenibacillus kribbensis AP-2 strain used in the present invention .

Figure 5 is, when a single treatment ferric chloride (FeCl ₃₎ in river water and, after treated with ferric chloride (FeCl ₃₎ Penny Bacillus Cri Ben AP cis -2 (Paenibacillus kribbensis AP-2) strain of It is a graph which compared turbidity in the case of processing the viscous material obtained from the culture.

FIG. 6 is obtained from cultures of Paenibacillus kribbensis AP-2 strains treated with aluminum chloride (AlCl ₃ ) alone and after treatment with aluminum chloride (AlCl ₃ ). It is a graph comparing the turbidity in the case of processing one viscous substance.

FIG. 7 shows the viscosity of aluminum and aluminum sulfate obtained from cultures of Paenibacillus kribbensis AP-2 when treated with aluminum sulfate (Al ₂ (SO ₄ ) ₃ ) alone in river water. It is a graph which compared turbidity and flocculating activity (FA) when the mixture of (alum) was processed.

FIG. 8 is a viscous material obtained by treating river water with polyaluminum chloride (PAC) or polyaluminum silica (PACS) alone, and in culture of a Paenibacillus kribbensis AP-2 strain. And a turbidity and aggregation activity when the mixture with PAC or PACS is treated.

FIG. 9 shows the viscous material and polyaluminum chloride (PAC) obtained from the treatment of the polyaluminum chloride (PAC) alone in the river water and the culture of the strain of Paenibacillus kribbensis AP-2. The graph shows a comparative observation of the control effect (%) on environmental factors (turbidity, TOC, COD and TN) in the case of treatment of a mixture of.

FIG. 10 shows the viscosity of polyvinyl chloride (PAC) and polyaluminum chloride (PAC) obtained from cultures of Paenibacillus kribbensis AP-2 strains treated with polyaluminum chloride (PAC) alone. The graph shows a comparative observation of the removal effect (%) of aluminum ions (Al ³⁺ ) and copper ions (Cu ²⁺ ) in river water when the mixture of ethylene is treated.

In the present invention, there is provided a composition for water treatment comprising a viscous material produced by the strain of Paenibacillus kribbensis AP-2 (Accession No. KCTC10421BP).

Further, in the present invention, Paenibacillus kribbensis AP-2 ( Paenibacillus kribbensis AP-2), which further comprises at least one of aluminum sulfate (alum), polyaluminum chloride (PAC) or polyaluminum chloride silica (PACS); Accession No. KCTC10421BP) provides a composition for water treatment comprising a viscous substance produced by the strain.

In the case of aluminum sulfate (alum), polyaluminum chloride (PAC), or polyaluminum chloride silica (PACS), which are additionally included in the present invention as a chemical synthetic material that is conventionally used as a water treatment agent, The type, content, etc. are determined according to the pollution degree of the water system to be treated, the substance to be treated, etc., which are obvious to those skilled in the art.

In a preferred embodiment of the present invention further comprising an alum, which is already used as a water treatment formulation, provides a water treatment composition in which the mixing ratio (weight ratio) of the viscous substance: alum produced by the strain is 1:10 to 1: 100. .

In a preferred embodiment of the present invention further comprising polyaluminum chloride (PAC), which is widely used as a water treatment agent, the mixing ratio (weight ratio) of the viscous substance: polyaluminum chloride produced by the strain is 1:10 to 1 It provides a composition for water treatment of 200.

In a preferred embodiment of the present invention further comprising polyaluminum chloride (PACS), which is conventionally used as a water treatment formulation, the mixing ratio (weight ratio) of the viscous material: polyaluminum chloride silica produced by the strain is 1:10 to It provides a water treatment composition of 1: 200.

The composition for water treatment of the present invention is useful for removing heavy metal ions (for example, Al ^{+ 3} , Cu ⁺² , Cr ⁺⁶ , Cd ⁺² , Zn ^+2, etc.) contained in the water system. The removal ability of Al ⁺³ and Cu ⁺² is excellent.

Further, in another aspect of the present invention, the method comprising the step of administering a viscous substance produced by the strain of Paenibacillus kribbensis AP-2 (Accession No. KCTC10421BP) or a water treatment composition comprising the same to the water system Provide a water treatment method.

In one preferred embodiment of the above water treatment method of the present invention, at least one of alum, polyaluminum chloride (PAC) or polyaluminum chloride silica (PACS) is added to the viscous material produced by the strain or a water treatment composition comprising the same. It provides a water treatment method characterized in that it is included.

In addition, in one embodiment of the water treatment method of the present invention, prior to the step of administering the viscous material produced by the strain to the water system, aluminum chloride (AlCl ₃ ), ferric chloride (FeCl ₃ ), calcium chloride (CaCl ₂ ) or potassium chloride (KCl), the method further comprises the step of administering. At this time, the type and dosage of aluminum chloride (AlCl ₃ ), ferric chloride (FeCl ₃ ), calcium chloride (CaCl ₂ ) or potassium chloride (KCl) are determined according to the pollution degree of the water system to be treated and the substance to be treated. This is obvious to those skilled in the art.

The "water treatment composition" of the present invention may include a variety of water treatment agents, solvents, additives, and the like that are commonly used in the treatment of water systems, including contaminated constant water, heavy water, sewage, and the like, which are common in the art. It is obvious to the technician.

As used herein, the term "viscous substance produced by the strain of Paenibacillus kribbensis AP-2; Accession No. KCTC 10421BP" refers to a viscous substance that is metabolized intracellularly and released extracellularly when the strain is cultured. Chemical analysis and collection method thereof is described in detail in Examples 2 and 3 below.

The water treatment composition provided by the present invention and the water treatment method using the same, by using a viscous material produced by the strain of Paenibacillus kribbensis AP-2 (Accession No. KCTC10421BP), Compared to water treatment formulations and water treatment methods using chemical synthetic materials, it is more eco-friendly and safer to human body, and has excellent control ability of environmental factors (aggregation activity and turbidity, total nitrogen removal ability, chemical oxygen demand, etc.) and removal ability of heavy metals. .

The following examples illustrate the invention in more detail, and these examples should not be construed as limiting the scope of the invention.

Example

Example 1 Isolation of Strains Used in the Present Invention

Water and soil were collected from polluted water systems such as contaminated soils and wastewater treatment plants in northern Gyeongsangbuk-do and used as samples for the separation of new microorganisms.

First, in order to select microorganisms that secrete a lot of viscous substances, 10 g / l of glucose, 2 g / l of ammonium nitrate, 0.8 g / l of potassium phosphate 1, 0.6 g / l of potassium diphosphate 2, calcium carbonate Sterile saline solution using a plate medium containing 0.3 g / l, Soyton 0.1 g / l, Tryptone 0.1 g / l, Yeast extract 0.1 g / l, Magnesium sulfate 0.05 g / l and Manganese sulfate 0.05 g / l The suspension prepared from the sample was smeared in three steps by dilution. The plated petri dishes were incubated for 1 to 3 days in an incubator at 30 ° C., and 20 kinds of microorganisms were isolated to produce a lot of viscous materials while showing excellent growth on plate medium.

After inoculating the separated 20 kinds of microorganisms in the same complex liquid medium, the culture medium was plated on the solid medium again to select 10 strains having good single colony formation and production of viscous substances. Then, the selected strains were placed in a culture test tube and incubated at 30 ° C. for 48 hours using the complex medium, and then the microorganisms forming a single colony were transferred to a test tube made of a slope medium and stored at 4 ° C. or 25% glycerol. Put and stored at -20 ℃ to use as needed.

10 microorganisms of the primary screened were inoculated by 2% (v / v) by using the above-described mixed liquid medium and shake-rotated at 30 ° C., and the aggregation activity was compared by using kaolin clay. Finally, a novel strain with good growth and good aggregation activity was finally identified and named as Paenibacilluskribbensis AP-2, which was deposited with the Korea Institute of Science and Technology Gene Bank. (Accession Number-KCTC10421BP; Deposit Date-February 10, 2003).

Looking at the photograph taken using a scanning electron microscope for the above strain, it can be seen that the identified strain has a form that is secreted to the outside of the cell after a certain viscous material is metabolized and produced in the cell (see : 1).

Example 2: Penivacillus crivensis api-2 Paenibacillus kribbensis Analysis of Viscous Substances Produced by AP-2)

After diluting the culture obtained by culturing the strain, the supernatant obtained by centrifugation was poured with cold ethanol (98%), which is three times the volume of the culture solution, to remove the white precipitate of pure viscous material, which is formed. After thawing it was dried by lyophilization. After the concentration of the sample thus obtained was about 1%, 2N trifluoroacetic acid was added, and hydrolysis was induced at high temperature and high pressure for 30 minutes, after cooling, neutralized, and the salt was removed to obtain a sample.

Sample analysis was predicted as per constituent using an RI analyzer (RID-6A, Shimadzu Co., Japan) through a carbopak NA1 column (flow rate-0.25 ml / min, column chamber temperature-room temperature) by High Pressure Liquid Chromatography (HPLC) As a result of analysis according to the time delay compared to the standard sugar, the viscous substance produced by the strain is a complex polysaccharide containing glucose, mannose, glucuronic acid and fucose in a molar ratio of 5: 1.86: 1.4: 1. (See FIG. 2).

In addition, in order to measure the molecular weight of the complex polysaccharides described above, the molecular weight of the complex polysaccharides prepared as 0.3% (w / v) using a PL-GFC 1000 Å column and using several dextran by molecular weight as standard carbohydrates It turned out to be about 230,000 dalton.

Example 3: Penivacillus crivensis api-2 ( Paenibacillus kribbensis Functional group analysis of viscous substances produced by AP-2)

In order to investigate the binding method of the complex polysaccharide produced in the strain and the functional group of the structural component, infrared spectroscopic analysis using a KBr pellet method [Model-Infiniti 60MI (Mattson, USA); Spectral range-400 to 4000 cm ^-1 ; Beam splitter-KBr] was obtained, as shown in FIG.

The complex polysaccharides produced in the strains were hydroxyl groups (-OH groups) at 3400 cm ^-1 , CH stretching at 2900 cm ^-1 , and glucosamine at 1600 cm ^-1 , which are common peaks in sugars and proteins. as show in the COC bond in R-NH ₂ group, and 1400cm-specific absorption band for generating possible glucan by the CH ₂ bond in the ^-1, 1080cm ^-1, which would normally be on a carbohydrate, an active group of the hydroxyl group It was confirmed that a carboxyl group, an amino group and the like were contained.

Example 4: Penivacillus crivensis api-2 Paenibacillus kribbensis Aggregation Activity of Cultures of AP-2)

A predetermined amount of the culture obtained by culturing the strain at 30 ° C. using the complex liquid medium described in Example 1 in a 5 L-fermentation tank was collected, and the final pH, viscosity, strain growth and aggregation activity according to the culture time were obtained. Investigate. At this time, the aggregation activity was measured according to a method commonly used in the art. {See: Yokoi H. et al., 1995, Characteristics of biopolymer flocculant produced by Bacillus sp. PY-90, J. Ferment. Bioeng. 79, 378- 380}.

As can be seen in Table 1 and Figure 4, the final pH, viscosity, growth and aggregation activity was changed according to the incubation time, especially cohesive activity of the viscous substance produced when incubated for about 48 hours to 60 hours This was found to be the highest. In particular, the coagulation activity of the viscous substances produced in Paenibacillus kribbensis AP-2 measured in this experiment was observed up to about 110, which is a chemical commonly used as a water treatment agent. It was also shown to be superior to the cohesive activity of the synthetic materials (eg, about 71 for alum).

Changes in Characteristics of Cultures of Paenibacillus kribbensis AP-2 with Culture Time Incubation time (hours) Final pH Viscosity (× 10 ³ ) Cell growth (OD, A ₆₆₀ ) Coagulation activity (F.A.) 12 5.92 1.02 0.15 39.66 24 5.75 1.85 0.28 49.65 36 5.64 2.03 1.25 99.65 48 5.45 2.63 2.54 110.77 60 5.40 2.88 3.45 110.77 72 5.52 2.87 2.85 99.65 84 5.60 2.85 2.24 82.99 100 5.78 2.75 2.24 66.32

Example 5: Penivacillus crivensis api-2 Paenibacillus kribbensis Culture of AP-2) and Ferric Chloride (FeCl) ₃ Agglomeration effect when using

After the different cases and, concentrations in the river water 1ℓ: (0.085 absorbance), only treatment with a ferric chloride (FeCl ₃₎ at different concentrations was added ferric chloride (FeCl ₃₎ turbidity of 53.2 NTU Turbidity when treated with 3 ml of the viscous material obtained from a culture of Penibacillus kribbensis AP-2 was compared (see FIG. 5).

The characteristics of the river water (1ℓ) used in this experiment are shown in Table 2 below:

Characteristics of river water used in this experiment Turbidity (NTU) TOC (ppm) COD (ppm) T-N (ppm) Alkalinity (ppm) Zeta potential (mV) Al content (ppm) Cu content (ppm) 53.2 9.73 2.52 3.57 48.03 2.53 2.47 1.26

As can be seen in Figure 5, compared with the case of using ferric chloride alone, the coagulant activity was higher when the culture of Paenibacillus kribbensis AP-2 was used together and the turbidity was removed. The effect was also excellent. In particular, the addition of about 100 to 125 ppm of ferric chloride (FeCl ₃ ) followed by the addition of 3 ml of the viscous material obtained from the culture of Paenibacillus kribbensis AP-2 It showed coagulation activity and turbidity removal effect.

Example 6: Penivacillus crivensis api-2 Paenibacillus kribbensis Culture of AP-2) and Aluminum Chloride (AlCl ₃ Agglomeration effect when using

In the same river water used in Example 5 1 L, the concentration of the aluminum chloride (AlCl ₃ ) was treated alone, and after the addition of aluminum chloride (AlCl ₃ ) at different concentrations, after the addition of Penicillus cribensis Turbidity when treated with 3 ml of the viscous material obtained from the culture of Apen ( Paenibacillus kribbensis AP-2) was compared with each other (see Fig. 6). The properties of the river water used in this experiment are shown in Table 2 above.

As can be seen in Figure 6, compared with the case of using aluminum chloride alone, the coagulant activity was higher when the culture of Paenibacillus kribbensis AP-2 was used together and the turbidity removal effect was also higher. Excellent. In particular, the highest flocculation activity and turbidity removal effect were added when 3 ml of viscous material obtained from the culture of Paenibacillus kribbensis AP-2 was added after the addition of about 10 to 40 ppm of aluminum chloride. Showed.

Example 7: Coagulation Effect of "A Mixture of Viscosity / Aluminum Sulfate (M / Alum) of Penivacillus cribensis epi-2"

When 1 L (53.2 NTU) of the same river water used in Example 5 was treated with aluminum sulfate (Al ₂ (SO ₄ ) ₃ , Alum), which has been widely used as a water treatment formulation, alone, and Phenibacillus cribensis Turbidity and flocculating activity when 3 ml of a viscous substance obtained from the culture of Paenibacillus kribbensis AP-2 and a mixture of aluminum sulfate (mixing ratios are described in the table below) were treated. FA) were compared with each other (see FIG. 7). The properties of the river water used in this experiment are shown in Table 2 above, and the comparison results are shown in Table 3 below.

Turbidity (NTU) Coagulation activity (F.A.) Alum sole 7.30 71.57 M / Alum (Strain Viscosity: Alum Content Ratio) 1:10 5.58 88.24 1:30 5.87 79.14 1:50 5.72 79.14 1:70 5.61 131.09 1: 100 4.95 131.09

As can be seen in Table 3 and Figure 7, compared to the case of using alum alone, a mixture of alum and culture of Paenibacillus kribbensis AP-2 in the above ratio When used, the coagulation activity and turbidity removal effect was found to be excellent.

Example 8: A mixture of viscous / polyaluminum chloride of Penivacillus cribensis epi-2 (M / PAC) or a mixture of viscous / polyaluminum silica of Penivacillus cribensis epi-2 (M / PACS) Coagulation effect of "

When 1 liter (53.2 NTU, absorbance 0.085) of the same river water used in Example 5 was treated with polyaluminum chloride (PAC) or polyaluminum silica (PACS), which is widely used as a water treatment formulation, alone (30 ppm each). ; When a mixture of viscous matter and polyaluminum chloride (PAC) obtained in a culture of Penivacillus cribensis epi-2 was treated; And turbidity, absorbance and flocculating activity (FA) in the case of treatment of the mixture of the slime and the polyaluminum chloride (PACS) obtained from the culture of Penivacillus cribensis epi-2. Compared. The properties of the river water used in this experiment are shown in Table 2 above, and the comparison results are shown in Table 4 and FIG. 8 below.

Turbidity (NTU) Absorbance (ABS) Coagulation activity (F.A.) PAC alone 4.6 0.006 154.9 PACS only 5.7 0.008 113.2 M / PAC (Strain Viscosity: PAC Content Ratio) ^# 1: 1 6.2 0.011 79.1 1: 3 6.2 0.011 79.1 1: 5 6.1 0.008 113.2 1: 7 5.8 0.008 113.2 1:10 5.4 0.007 131.1 1:30 5.1 0.007 131.1 1:50 4.9 0.006 154.9 1:70 4.1 0.006 154.9 1: 1001: 200 4.54.8 0.0060.007 154.9131.1 M / PACS (strain viscosity: PACS content ratio) ^# 1: 1 10.9 0.018 43.8 1: 3 9.3 0.015 54.9 1: 5 9.2 0.015 54.9 1: 7 8.5 0.013 65.2 1:10 7.5 0.011 79.1 1:30 6.7 0.010 88.2 1:50 6.6 0.009 99.3 1:70 5.4 0.008 113.2 1: 1001: 200 4.75.7 0.0070.009 131.199.3

{In the above table, the total amount of #-M / PAC or M / PACS mixture is 30 μl each, and according to the mixing ratio, the culture viscous material of the strain is mixed with PAC or PACS (for example, 1:10). PAC content is 27.0 ppm, and the content of PAC at 1: 200 is 29.85 ppm)}.

As can be seen in Table 4 and Figure 8, in terms of turbidity removal effect and flocculation activity, compared with the case of using polyaluminum chloride (PAC) or polyaluminum silica (PACS) alone, penicillus cribene When used in combination with a viscous substance of Cien API -2 ( Paenibacillus kribbensis AP-2) showed at least a similar or better effect. Particularly, when the mixing ratio of the viscous material of the peniebacillus kribbensis AP-2 and polyaluminum chloride (PAC) or polyaluminum silica (PACS) is 1:10 to 1: 200 (weight ratio), Coagulation activity and turbidity removal effect was found to be more excellent.

Example 9: Effect on Environmental Factors of "Viscous Matter / Mixture of Polyaluminum Chloride (M / PAC) of Penivacillus Cribensis API-2"

When 1 liter of the same river water used in Example 5 was treated with 30 ppm of polyaluminum chloride (PAC), which is widely used as a water treatment agent, alone, and Paenibacillus kribbensis AP-2. 30 μl of a mixture of a viscous substance and a polyaluminum chloride (PAC) obtained from the culture of (wherein the ratio of strain viscous: PAC is 1:70, PAC content-29.57ppm) to environmental factors The control effect was compared.

Specific properties of the river water used in this experiment are shown in Table 2 above. In this experiment, four environmental factors, including chemical oxygen demand (COD), total organic carbon (TOC), turbidity, and total nitrogen (TN), were compared and observed. Same as 9.

Turbidity (NTU) TOC COD T-N River water 53.2 9.73 2.52 3.6 PAC 2.44 (control rate-95.4%) 6.60 (control rate-32.1%) 0.94 (control rate-70.5%) 1.98 (control rate-34.3%) M / PAC mixture (strain viscous: PAC = 1:70) 2.13 (control rate-96.0%) 4.48 (control rate-53.9%) 0.74 (control rate-79.5%) 1.69 (control rate-40.3%)

As can be seen in Table 5 and Figure 9, compared to the case of using the polyaluminum chloride (PAC) which is most commonly used as a conventional water treatment formulation of the Penibacillus cribensis AP-2 ( Paenibacillus kribbensis AP-2) When used in combination with the viscous material obtained from the culture was more environmentally friendly and showed an excellent water treatment effect.

Example 10: Heavy metal removal effect of "mixture of viscous / aluminum polyaluminum (M / PAC) of Penivacillus cribensis epi-2"

When 1 liter of the same river water used in Example 5 was treated with 30 ppm of polyaluminum chloride (PAC), which is widely used as a water treatment agent, alone, and Paenibacillus kribbensis AP-2. 30 μl of a mixture of a viscous substance and a polyaluminum chloride (PAC) obtained in the culture of (wherein the ratio ratio of strain culture: PAC is 1:70, PAC content-29.57ppm) to the river water, The effect of removing the aluminum and copper ions contained was compared.

As can be seen in Table 6 and FIG. 10 below, Paenibacillus kribbensis AP-2 compared to the case of using polyaluminum chloride (PAC), which is most commonly used as a water treatment formulation, alone. When mixed with the viscous material obtained from the culture of the heavy metals, including aluminum and copper was shown to have a large effect.

Al ⁺³ concentration (ppm) Cu ⁺² concentration (ppm) River water 2.472 1.264 PAC 0.558 (Removal rate-77.4%) 1.258 (Removal rate-0.5%) M / PAC mixture (strain viscosity: PAC = 1:70) 0.369 (Removal rate-85.1%) 1.206 (Removal rate-4.6%)

Claims (10)

A composition for water treatment comprising a viscous substance produced by a strain of Paenibacillus kribbensis AP-2 (Accession No. KCTC10421BP). The water treatment composition of claim 1, further comprising at least one of alum, polyaluminum chloride (PAC), or polyaluminum chloride silica (PACS). The water treatment composition of claim 2, further comprising an alum, wherein a mixing ratio (weight ratio) of the viscous substance: alum produced by the strain is 1:10 to 1: 100. The method according to claim 2, wherein the polyvinyl chloride (PAC) further comprises, the mixing ratio (weight ratio) of the viscous material: polyaluminum chloride produced by the strain is characterized in that 1:10 to 1: 200. Water treatment composition. The method according to claim 2, wherein when the polysilica chloride (PACS) is further included, the mixing ratio (weight ratio) of the viscous material: polyaluminum chloride silica produced by the strain is 1:10 to 1: 200. Composition for water treatment. The water treatment composition according to claim 1 or 2, which is used for removing heavy metal ions contained in the water system to be treated. The composition for water treatment according to claim 6, wherein the heavy metal ion is aluminum ion (Al ⁺³ ) or copper ion (Cu ⁺² ). A water treatment method comprising the step of administering a viscous substance produced by a strain of Paenibacillus kribbensis AP-2 (Accession No. KCTC10421BP) or a water treatment composition comprising the same to an aqueous system. The method of claim 8, The water treatment method characterized in that the viscous material produced by the strain or a water treatment composition comprising the same further comprises at least one of alum, polyaluminum chloride (PAC) or polyaluminum chloride silica (PACS). The method according to claim 8, wherein before the step of administering the viscous material produced by the strain or the water treatment composition comprising the same to the aqueous system, aluminum chloride (AlCl ₃ ), ferric chloride (FeCl ₃ ), calcium chloride (CaCl ₂ ) or Water treatment method further comprises the step of administering at least one of potassium chloride (KCl).