KR100419474B1 - New Variety "Jeogtoma"-Inbreeding of Jeju Cymbidium Kanran Makino - Google Patents

New Variety "Jeogtoma"-Inbreeding of Jeju Cymbidium Kanran Makino Download PDF

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KR100419474B1
KR100419474B1 KR10-2001-0052248A KR20010052248A KR100419474B1 KR 100419474 B1 KR100419474 B1 KR 100419474B1 KR 20010052248 A KR20010052248 A KR 20010052248A KR 100419474 B1 KR100419474 B1 KR 100419474B1
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jeju
new varieties
jeogtoma
inbreeding
new variety
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KR20030017959A (en
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고태신
양상호
성문석
문정수
강명선
김광호
소인섭
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제주도(농업기술원)
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/62Orchidaceae [Orchid family]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/021Methods of breeding using interspecific crosses, i.e. interspecies crosses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02Flowers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

본 발명은 초형이 수엽(垂葉) 형태이고, 초세가 강건하고, 내서성이 강하며, 개화시기가 10∼11월로 연지색(10RP 3/4)이고 화형은 만세피기로서 우수한 제주한란 신품종에 속하는 식물 '적토마'를 제공하는 뛰어난 효과가 있다.The present invention is the leaf type in the form of a leaf, the super fine strength, the strong resistance, the flowering period is from October to November, light green color (10RP 3/4), and the burn type is excellent in Jeju Hallan new varieties. It has an excellent effect of providing the plant 'Red Tomato'.

Description

제주한란 신품종 '적토마' 식물{New Variety "Jeogtoma"-Inbreeding of Jeju Cymbidium Kanran Makino}New Variety "Jeogtoma" -Inbreeding of Jeju Cymbidium Kanran Makino}

본 발명은 제주한란(JejuCymbidium kanranMakino) 신품종 '적토마(赤馬)'라 명명한 식물에 관한 것이다. 더욱 상세하게는, 본 발명은 초형이 수엽(垂葉) 형태이고, 초세가 강건하고, 내서성이 강하며, 개화시기가 10∼11월로 연지색(10RP 3/4)이고 화형은 만세(萬歲)피기로서 우수한 신품종으로 고정된 제주한란 신품종에 속하는 식물 '적토마'에 관한 것이다.The present invention is Jeju Cymbidium kanran Makino new varieties' red tomato (赤) It is about a plant named '馬)'. More specifically, in the present invention, the supermodel is in the form of a leaf, the superfine strength is strong, the resistance to resistance is strong, the flowering time is from October to November, light green color (10RP 3/4), and the burn type is long lived (萬歲) Jeju Hanran is a plant that belongs to the new varieties, which are fixed as new varieties.

한란(寒蘭 :Cymbidium KanranMakino)으로 부르는 심비디움의 원종(原種)은 대체로 한국, 중국, 일본 등 온대 동남아시아에서 자생하는 지생종(地生種)이며 원예품종은 이들 원종들 간을 교배하거나 이들 원종과 일본종, 중국종과의 교배에 의해 육성된 것들이다.The species of symbidium, called Cymbidium Kanran Makino, is an indigenous species that is native to temperate Southeast Asia, such as Korea, China, and Japan. These were fostered by crosses with Japanese and Chinese species.

한편, 한란의 대량 생산이 가능한 것은 식물조직배양(Plant Tissue Culture)기법 특히, 교배된 배(胚)를 무균파종으로 발아하여 배양·증식함으로써 얻을 수 있는 영양계(營養系) 즉, clone culture의 기술에 의해서이다.On the other hand, it is possible to mass-produce the eggs in the plant tissue culture technique, in particular, the nutrient system, which is obtained by germinating and cultivating cultivated embryos by aseptic seeding, ie, clone culture. By technology.

특히, 한란은 상기 clone culture 기술에 의해 대량 생산이 가능하고 새로운 품종의 육성이 용이해지고 그 결과 저렴한 가격에 의해 소비자의 수요를 만족할 수가 있다. 따라서, 본 발명의 목적은 수요자가 만족할 수 있는 제주한란의 신품종을 값싸게 대량 제공함에 있다.In particular, the egg can be mass-produced by the clone culture technology, and it is easy to cultivate new varieties, and as a result, it can satisfy the demand of consumers by the low price. Therefore, an object of the present invention is to provide a large amount of cheap new varieties of Jeju Hanlan that can satisfy the consumer.

본 발명의 상기 목적은 기존의 제주한란을 모본과 부본으로 선택하여 이를 교잡(Cross hybridization)하고 화색(花色)은 모본과 같고, 잎의 자세(草姿)는 부본과 같으며, 꽃피는 시기(開花期)가 모·부본과 같은 신품종을 무균종자 발아에 의하여 육성한 후 그 영양체를 계대배양함으로써 달성하였다.The object of the present invention is to select the existing Jeju Hanran as a parent and a copy, cross hybridization and the color (花色) is the same as the mother, the leaf posture (grass) is the same as the bourbon, flowering time ( This was achieved by subculture of the nutrients after cultivation of new varieties such as mother and stalk by germination of sterile seeds.

도1은 본 발명의 제주한란 신품종에 속하는 식물의 꽃, 잎, 줄기, 도2는 꽃모양의 정면, 도3은 꽃모양의 옆면 표현형질을 나타낸 사진도Figure 1 is a flower, leaf, stem of the plant belonging to the Jeju Hanran new varieties of the present invention, Figure 2 is a front view of the flower shape, Figure 3 is a photograph showing the lateral phenotype of the flower shape

본 발명은 실생종자의 무균종자 발아단계와; 배양된 clone cluture의 계대배양단계로 구성된다.The present invention provides aseptic seed germination step of live seed; It consists of subcultures of cultured clone cluture.

이하, 본 발명의 구체적인 구성 및 작용을 실시 예를 들어 설명하지만, 본 발명의 권리범위가 이들 실시 예에만 한정되는 것은 아니다.Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.

실시예 1Example 1

제주 자한란(♀) × 제주 청한란(♂) 계통이 교배된 무균종자 발아 및 근경생장Germination and Muscular Growth of Aseptic Seeds Crossed with Jeju Jahan Ran (♀) × Jeju Cheonghan Ran (♂)

제주한란 실생종자를 발아시키기 위하여 MS(Murashige and Skoog Media)의 무기물 함량을 반으로 감량하고, 설탕(sugar) 30g, pH 5.3으로 조정한 후 한천(agar)이 첨가되지 않은 액체 배지를 제조하였으며 회전진탕에서 무균적으로 발아시켜 보통의 방법으로 비배(肥培) 관리하여 생장시켰다.In order to germinate the seeds of Jeju Hanran, the mineral content of MS (Murashige and Skoog Media) was reduced by half, and after adjusting to 30 g of sugar and pH 5.3, a liquid medium without agar was prepared and rotated. Germinated aseptically in shake and grown by fertilization in the usual way.

실생종자는 모본(母本)으로 제주한란(화색이 연지색) 계통을, 부본(父本)으로 제주한란(화색이 연두색) 계통을 교배후 1년된 완숙 종자를 선택하였다.Seedlings were selected for 1-year-old ripening seed after breeding Jeju Hanlan (light color green) line as a parent and Jeju Hanlan (light green color) line as a copy.

종자발아 반응의 차이는 種, 品種, 交配組合에 따른 遺傳的 差異에 기인하거나 종자의 성숙도 또는 배의 충실도에 따라 반응이 달라질 수도 있는데 濟州寒蘭의 自家 및 他家交配 종자를 0.1N KOH(종피연화처리제) 용액과 Wilson's 용액(CaCl2 10g을 증류수 140㎖를 첨가시킨 후 여과지로 걸러낸 용액)에 침지하여 Voltex-mixer를 이용 소독 및 연화처리 한 후 배지에 파종하면 발아소요기간이 1년 이상에서 4∼6개월 이내로 종자발아가 빠르고 발아개체수도 많이 발생할 수 있는 종자배양기술을 확립하였다.Differences in seed germination may be due to a variety of strains, varieties, and combinations, or the response may vary depending on the maturity of the seeds or the fidelity of the embryos. Treatment agent) solution and Wilson's solution (10g of CaCl2 was added to 140ml of distilled water and filtered through a filter paper), soaked in Voltex-mixer, and then seeded in the medium. We have established a seed culture technology that enables seed germination to occur quickly and to generate a large number of germination within 6 months.

실시예 2Example 2

제주한란 근경(rhizome)에서 신초 분화Shoot Eruption in the Jeju Hanran Rhizome

濟州寒蘭의 경우 급속한 개체 증식을 위해서 근경분화배지를 조성할 때 植物生長調節物質(BA)를 첨가하는데 배양중 배지가 심하게 갈변되어 정상적인 생육이 저해되고, 植物生長調節物質의 효과가 나타나지 않는데 이러한 현상을 방지하기 위하여 산화방지에 효과가 있는 항산화제(Polyvinylpyrrolidone)을 선발하였고, 근경에서 신초 분화를 위하여 다음의 배지를 사용하였다.In the case of 濟 州 寒 蘭, 조성 物 生長 調節 物質 (BA) is added to form a root differentiation medium for rapid growth of the individual, and the medium is severely browned during culture, which inhibits normal growth and does not show the effect of 植物 生長 調節 物質. Antioxidant (Polyvinylpyrrolidone) was selected to prevent the antioxidant, and the following medium was used for shoot differentiation in the rhizome.

실시예 3Example 3

제주한란 기내육묘Jeju Hanlan In-flight nursery

조직배양을 이용하여 묘를 생산할 때 근경으로부터 개체 발생까지는 문제가 없으나 순화단계에서 큰 애로를 겪고 있는 경향인데 기존의 東洋蘭系統들의 근경배양은 왕성한 근경 생장에 비해 식물체의 크기가 작고 촉수가 많이 발생하지 않으며, 순화단계에서는 생존율이 낮은 경향이어서 충실하고 건강한 묘를 생산하기 위해서 배지에 천연산물 등을 첨가하여 배양하는 방법 등 배양병내에서 우량한 배양묘를 생산할 수 있는 배양기술을 개발하였다.When producing seedlings using tissue culture, there is no problem from the rhizome to the occurrence of the individual, but there is a tendency to suffer a great difficulty during the acclimatization stage. In the purifying stage, the survival rate was low, and thus, in order to produce a faithful and healthy seedling, a culture technique capable of producing superior cultured seedlings in a culture bottle was developed, such as culturing by adding natural products to the medium.

본 발명 제주한란 신품종 식물체를 유도하고 발근을 위한 배지는 아래 하이포넥스 배지(Kyoto Ⅱ)를 사용하고 여기서 바나나즙과 감자즙을 첨가하여 생장을 촉진시켰다.Inventive Jeju Hanlan new varieties of plants and rooting medium for the rooting was used below Hyphenex medium (Kyoto II), where banana juice and potato juice were added to promote growth.

한편, 근경으로부터 신초(shoot)가 분화되면 근경을 제거 한 신초만 육묘용 500㎖ flask에 이식하되 500㎖ 플라스크에 5주를 기준으로 육묘하여 품종을 고정하였으며, 본 발명 제주한란 신품종 '적토마' 식물의 원예적 특성은 다음 표와 같다.On the other hand, when shoots are differentiated from the roots of the shoots, only the shoots from which the roots are removed are transplanted into a 500 ml flask for seedlings, but the seedlings are fixed by seeding the seedlings based on 5 weeks in a 500 ml flask. The horticultural characteristics of are shown in the following table.

실시예 4Example 4

제주한란 신품종 식물의 순화Purification of new varieties of plants in Jeju Hanlan

계대배양하여 1년 정도 경과한 벌브경 12mm 내외의 유묘는 flask에 물을 넣어 핀셋으로 배지를 허물고 적취하여 배지를 증류수로 깨끗이 제거한다.Seedlings around 12 mm bulb diameter that passed about one year after passage are added to the flask, and the medium is removed by tweezers, and the medium is removed with distilled water.

순화조건은 자연광의 10% 정도인 5,000∼10,000lux가 되도록 하고 대기습도는 차광하여 통기성이 양호한 바구니에 식재하고 70∼80%를 유지하여 건조되지 않도록 주의하였다.Purifying conditions were set to 5,000 to 10,000 lux, which is about 10% of natural light, and the atmospheric humidity was shaded, planted in a basket with good ventilation, and 70-80% was maintained so as not to be dried.

순화된 본 발명 제주한란 신품종 '적토마' 식물의 개화된 전체의 모습, 꽃모양의 정면 및 옆면은 사진도 1, 2, 3과 같으며 기존의 한란계통과 현저히 구별되는 연지(10RP 3/4)색의 화형이 만세피기를 나타내었다.The entire appearance, flower front and side of the purified Jeju Jeju new varieties of 'red toma' plants are as shown in Figures 1, 2, and 3, and are distinctly different from the existing Hanlan system (10RP 3/4). Fire extinguisher showed

이상과 같이 본 발명은 실시 예를 통하여 설명한 바와 같이, 본 발명은 초형이 수엽(垂葉) 형태이고, 초세가 강건하고, 내서성이 강하며, 개화시기가 10∼11월로 연지색(10RP 3/4)을 가지는 만세피기로서 우수한 신품종에 속하는 제주한란 신품종 식물을 제공하는 효과가 있으므로 식물 신품종 육성산업상 매우 유용한 발명인 것이다.As described above, the present invention, as described through the embodiment, the present invention is the ultra-large leaf shape, ultra-strong, strong resistance, the flowering time of October to November light color (10RP 3 / 4) is a very useful invention in the plant new breeding industry because it is effective to provide a new varieties of plants of Jeju Hanlan belonging to the new varieties excellent as a mansion piggy.

Claims (1)

기존의 제주 자한란(♀) × 제주 청한란(♂) 계통의 교배종자를 무균발아 생장시킨 다음 근경(rhizome)을 증식하고 근경으로부터 신초(shoot)가 분화되면 이를 flask에 분주 육묘하여 3대 이상 계대배양에 의하여 무성번식시켜 고정시킴을 특징으로 하는 원예적 특성을 가지는 제주한란 신품종에 속하는 '적토마' 식물Aseptic germination of the existing Jeju Jahan-lan (♀) × Jeju Cheonghan-lan (♂) strains is grown, and then the rhizome is multiplied, and when shoots are differentiated from the root, the seedlings are seeded and seeded in flasks for three or more passages. 'Red Toma' plants belonging to the new varieties of Jeju Hanlan with horticultural characteristics characterized by asexual propagation and fixation by 가) 잎의 길이(葉長) : 33cm 내외A) Length of leaves: around 33cm 나) 잎의 수(葉數) : 3 ∼ 4장B) Number of leaves: 3 to 4 다) 꽃의 수 : 3 ∼ 6개C) Number of flowers: 3 to 6 라) 꽃대의 길이 : 26.7 ∼ 39.8cmD) Length of stalk: 26.7 ~ 39.8cm 마) 꽃색(花色) : 연지색(10RP 3/4)E) Flower color: Flower color (10RP 3/4) 바) 꽃의 크기(花幅) : 6.7 ∼ 8.0cmF) Flower size: 6.7∼8.0cm 사) 개화기(開花期) : 10 ∼ 11월G) Flowering season: October to November 아) 품종의 용도 : 관상용H) Use of varieties: ornamental 자) 내병성 : 다소강함I) Resistance to disease: somewhat stronger
KR10-2001-0052248A 2001-08-25 2001-08-25 New Variety "Jeogtoma"-Inbreeding of Jeju Cymbidium Kanran Makino KR100419474B1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100700446B1 (en) * 2005-05-30 2007-03-27 학교법인 정의학원 New Cultivar of Cymbidium Miak

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KR100532767B1 (en) * 2003-10-31 2005-11-30 제주도(농업기술원) Selection of Cymbidium Kanran 'Su-Kwan' new variety

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100700446B1 (en) * 2005-05-30 2007-03-27 학교법인 정의학원 New Cultivar of Cymbidium Miak

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