KR100490150B1 - New variety of plant Cymbidium ENZAN CLASSIC Malibu girl - Google Patents

Abstract

The present invention has an excellent effect of providing a plant 'Anzan Classic Malive Girl' belonging to a new breed of excellent egg symbidium for eruption having a pink.

Description

New breed of plant Cymbidium ENZAN CLASSIC Malibu girl

The present invention relates to a new egg 'Cymbidium' new breed 'Anjan Classic Malive Girl' plant. More specifically, the present invention relates to a plant 'ENZAN CLASSIC Malibu girl' belonging to a new breed of poultry symbidium, which is fixed as an excellent new breed for differentiation with pink color.

The origin of the symbidium, known as the Yangran, is a ring species native to the highlands (800-1,500m) of tropical Southeast Asia, such as northern India, Burma, Thailand, and Vietnam. Sword-shaped leaves, usually called bulbs, have large stems, thick roots (about 0.5 cm in diameter), and roots such as several clusters of flowers in clusters of two or three rows, with long flowering periods. It is characteristic. Therefore, it is widely used for flower arrangements and small bouquets, and is the most popular breed for poultry in Korea. Of the approximately 70 species found so far, small symbidium originated from East Asia, which has been observed to erupt in Korea, China, and Japan for a long time, is regarded as horticultural eggs separately. Currently called symbidium or western symbidium and produced by differentiation and cut flowers are species found in Southeast Asia and Australia and transmitted to Europe in the 19th to early 20th centuries and horticultural breeds centered around them.

The breeding of symbidium began in England in 1889, and soon after breeding in Australia and the United States, and in 1930, breeding was carried out only between large tropical species. However, in 1935, large symbidium was bred in Geumneung-byeon, a type of oriental egg in Japan, and cultivated a robust small variety of polymorphism called 'mini'. Since then, breeding using oriental egg and tropical small symbidium has become active in the US and Australia.

Compared to the large symbidium, the whole plant including flowers are small and well bloomed, they are rich in color and bloom, are quick to bloom and easy to cultivate.

Korea's symbiotic cultivation was introduced in Japan in the 1970s, but initially it was a valuable and expensive hobby flower grown only by specific people.

With the development of the economy since the 1970s, new varieties imported from abroad attracted attention as high-grade cuts or differentiation, which led to the planting of symbidium by some of the flowering plants, plants, plants and vegetables. However, since it was reproduced by division, it was difficult to obtain seedlings in quantity or mask, and the market was limited. Therefore, symbidium production did not spread smoothly. Symbidium has been mass-produced because of the world's rapidly growing tissue culture technique, especially the mercury obtained by extracting, culturing and growing apical meristem. As technology spreads and they begin to be produced by differentiation. In particular, symbidium can be mass-produced by the mercury technology, and it is easy to cultivate new varieties, and as a result, it is possible to satisfy consumer demand by low price.

Accordingly, an object of the present invention is to provide a large amount of new varieties of symbidium which can be satisfied by the consumer at low cost.

The above object of the present invention is to select the existing egg symbidium as the parent and the bundling and hybridization and color (flower), leaf posture (plantation), flowering time (開花 期) aseptic seed species and other new varieties After cultivation by germination, the nutrients, especially spermatozoon, were extracted, tissue cultured, and subcultured.

The present invention provides aseptic seed germination step of live seed; Corrective cutting step; Fertility culture step; It consists of three passages of cultured Mericlone.

Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.

Example 1

Germination and growth of sterile seeds crossed with pretty elves (♀) × great flower ballerina (♂) strain

In order to germinate symbiotic seedlings, Knudson C medium was selected and the ratio of SO ₄ ^-2 , Ca ⁺² , NH ₄ ⁺ ions was lowered and the ratio of K ⁺ ions was increased. The embryo was managed by the method to grow. ingredient content KH ₂ PO ₄ 0.3 g / L Ca (NO ₃ ) ₂ .4H ₂ O 0.5 g / L NH ₄ NO ₃ 0.5 g / L MgSO _{4 7} H ₂ O 0.21 g / L Fe ₂ SO ₄ · 7H ₂ O 0.025 g / L MnSO ₄ 4H ₂ O 0.0075 g / L Sudang 20 g / L Agar 7.5 g / L

Seeds were selected for crossing the Pretty Elf line as the parent and the Great flower "Ballerina" line as the copy.

Sterilization of the seedling was sterilized in 10% Ca (ClO) ₂ solution for 20 minutes and then put into sterile water to use the seed precipitated using a magnetic stirrer.

Example 2

Composition of the initial tissue culture badge

Unlike the seed culture medium of Example 1, a medium prepared as follows was used in order to promote the initial growth of fertilization or fertilization of fertilization (莖 頂).

ingredient content KNO ₃ 0.50 g / L CaHPO ₄ 0.22 g / L KH ₂ PO ₄ 0.35 g / L (NH ₄ ) ₂ SO ₄ 0.51 g / L MgSO _{4 7} H ₂ O 0.21 g / L Fe ₂ (C ₄ H ₄ O ₆ ) ₃ 0.30 g / L Tryptone 2 g / L Sudang 20 g / L Agar 7.5 g / L

For fertility extraction medium, 8 g / L agar is added to the growth of PLB. pH was set at 5.5.

Example 3

Proliferation of Protoplasts (PLB)

The following KC medium was used for proliferation of protoplasts.

ingredient content KH ₂ PO ₄ 0.35 g / L Ca (NO ₃ ) ₂ .4H ₂ O 0.5 g / L (NH ₄ ) ₂ SO ₄ 0.51 g / L MgSO _{4 7} H ₂ O 0.21 g / L Fe ₂ SO ₄ · 7H ₂ O 0.030 g / L MnSO ₄ 4H ₂ O 0.0080 g / L Sudang 20 g / L Agar 7.5 g / L

Example 4

Induction and rooting of new plant species of the present invention

As a medium for inducing and rooting new varieties of the present invention, Knudson (1946) medium below was used, and activated carbon was added to promote rooting rate and ground and underground growth.

ingredient content KH ₂ PO ₄ 0.35 g / L Ca (NO ₃ ) ₂ .4H ₂ O 0.5 g / L (NH ₄ ) ₂ SO ₄ 0.51 g / L MgSO _{4 7} H ₂ O 0.21 g / L FeSO ₄ 7H ₂ O 0.030 g / L MnSO ₄ 4H ₂ O 0.0080 g / L ZnSO ₄ 4H ₂ O 0.0033 g / L CuSO ₄ 0.0002 g / L Sudang 20 g / L Agar 7.5 g / L

The media composition was prepared 100 times the liquid and stored in the refrigerator, but Ca ^{+ 2} and SO ₄ ^-2 , Mg ⁺² and PO ₄ ^- form an insoluble precipitate was added when used.

On the other hand, when shoots were differentiated from protoplasts, they were transplanted into 300 mL flasks for seedlings, but seeded at 20 weeks in 300 mL flasks and 40 weeks in 500 mL flasks. The horticultural characteristics of the new varieties of the present invention 'Anjan classical Malive girl' plants are shown in the following table.

Agency characteristic Phenotype leaf Leaf length 78 cm Number of leaves Chapter 11 Leaf width 3 cm Leaf color green Flower Number of flowers All 25 Length of stalk 68 cm Flower Color Pink (DIC process color guide 1000, P-452) Flower discoloration Pink on white border Flowering period January-February Use of varieties Eruption In the above table, the number of flowers having the color closest to the flower color in the shade without direct sunlight was written using the DIC process color guide 1000.

Example 5

Purification of new varieties of plants

Seedlings of about 15 cm, which have been passaged for about 3 months, are poured into a flask, and the medium is removed by tweezers, and the medium is removed with distilled water.

Purifying conditions were to be placed in a basket with good air permeability, shaded and air permeable to maintain a range of 70 ~ 80% so that the moisture is 5,000 ~ 10,000 lux, which is about 10 of the natural light.

The entire flowering state of the purified new varieties of the 'Anzan Classic Malive Girl' plant is shown in Figure 1, and shows a distinct pink color (DIC process color guide 1000, P-452) from the existing egg system.

As described above, the present invention has been described as an embodiment, the present invention is very useful invention in the new plant breeding industry because it has the effect of providing a plant belonging to a new breed of Symbidium excellent egg for differentiation with pink.

 1 is a photograph showing the phenotype of flowers, leaves, stems, etc. of plants belonging to a new breed of poultry symbidium of the present invention.

Claims (1)

Aseptic germination of existing pretty elves (♀) x Great Flower Ballerina (♂) strains is carried out, followed by extraction of the fertilization, proliferation of protoplasts (PLB), and shoots from the protoplasts. Anzan classical Malibu girl belonging to a poultry symbidium having the following horticultural characteristics, characterized in that the seeding and seeding seedlings in a flask to be asexual propagation by three or more passages. 1) Length of leaves: 78 cm 2) Number of leaves: 11 sheets 3) The width of the leaves: 3 cm 4) Leaf Color: Green 5) Number of flowers: 25 6) the length of the flower stalk: 68 cm 7) Flower Color: Pink (DIC process color guide 1000, P-452) 8) Flower discoloration (Nibul): Pink with white border 9) Blooming season: January to February 10) Use of varieties: for differentiation