KR19990046071A - New variety of plant cymbidium Aguri - Google Patents

New variety of plant cymbidium Aguri Download PDF

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KR19990046071A
KR19990046071A KR1019990009047A KR19990009047A KR19990046071A KR 19990046071 A KR19990046071 A KR 19990046071A KR 1019990009047 A KR1019990009047 A KR 1019990009047A KR 19990009047 A KR19990009047 A KR 19990009047A KR 19990046071 A KR19990046071 A KR 19990046071A
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aguri
length
plant
protoplasts
new
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KR100330123B1 (en
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김영우
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김영우
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/62Orchidaceae [Orchid family]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/60Flowers; Ornamental plants
    • A01G22/63Orchids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/021Methods of breeding using interspecific crosses, i.e. interspecies crosses
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02Flowers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 복숭아색을 가지는 복륜화로서 분화용의 우수한 양란 심비디움 신품종에 속하는 식물 "아구리"를 제공하는 뛰어난 효과가 있다.The present invention has an excellent effect of providing a plant "Aguri" belonging to a new breed of excellent egg yolk symbiosis for the differentiation as a peach-colored abalone.

Description

양란 심비디움 신품종 아구리 식물 {New variety of plant cymbidium Aguri}New egg of Symbidium Agri plant {New variety of plant cymbidium Aguri}

본 발명은 양란 심비디움(Cymbidium) 신품종 "아구리" 식물에 관한 것이다. 더욱 상세하게는, 본 발명은 복숭아색을 가지는 복륜화로서 분화용의 우수한 신품종으로 고정된 양란 심비디움 신품종에 속하는 식물 "아구리(Aguri)"에 관한 것이다.The present invention relates to a new egg "Cymbidium" varieties "Aguri" plants. More specifically, the present invention relates to a plant "Aguri" belonging to a new breed of poultry symbidium, which is a peach-colored abalone, which is fixed as an excellent new breed for differentiation.

양란(洋蘭)으로 부르는 심비디움의 원종(原種)은 대체로 인도 북부, 버마, 타이, 베트남 등 열대 동남아시아의 고지대(800∼1,500m)에 자생하는 반지생종(半地生種)이며 원예품종은 이들 원종들 간을 교배하거나 이들 원종과 일본종, 중국종과의 교배에 의해 육성된 것들이다.The origin of the symbidium called the egg eggs is a ring species native to the highlands (800-1,500m) of tropical Southeast Asia, such as northern India, Burma, Thailand and Vietnam. They are bred between the native species or by breeding these species with Japanese and Chinese species.

한편, 심비디움의 대량 생산이 가능한 것은 조직배양(Tissue Culture)기법 특히, 경정분열조직(apical meristem)을 적출하여 배양·증식함으로써 얻을 수 있는 영양계(營養系) 즉, mericlone의 기술에 의해서이다.On the other hand, mass production of symbidium is possible by tissue culture techniques, in particular by the technology of nutrient system, that is, mericlone, which can be obtained by extracting, culturing and growing apical meristem.

특히, 심비디움은 상기 mericlone 기술에 의해 대량 생산이 가능하고 새로운 품종의 육성이 용이해지고 그 결과 저렴한 가격에 의해 소비자의 수요를 만족할 수가 있다. 따라서, 본 발명의 목적은 수요자가 만족할 수 있는 심비디움의 신품종을 값싸게 대량 제공함에 있다.In particular, symbidium can be mass-produced by the mericlone technology, and it is easy to cultivate new varieties, and as a result, it is possible to satisfy consumer demand by low price. Accordingly, an object of the present invention is to provide a large amount of new varieties of symbidium which can be satisfied by the consumer at low cost.

본 발명의 상기 목적은 기존의 양란 심비디움을 모본과 부본으로 선택하여 이를 교잡하고 화색(花色), 잎의 자세(草姿), 꽃피는 시기(開花期)가 모·부본과 다른 신품종을 무균종자 발아에 의하여 육성한 후 그 영양체 특히, 경정(莖頂)을 적출하여 이를 조직배양한 후 계대배양함으로써 달성하였다.The above object of the present invention is to select the existing egg symbidium as the parent and the bundling and hybridization and color (flower), leaf posture (plantation), flowering time (開花 期) aseptic seed species and other new varieties After cultivation by germination, the nutrients, especially spermatozoon, were extracted, tissue cultured, and subcultured.

도 1 은 본 발명의 양란 심비디움 신품종에 속하는 식물의 꽃, 잎, 줄기 등의 표현형질을 나타낸 사진도.1 is a photograph showing the phenotype of flowers, leaves, stems, etc. of plants belonging to a new breed of poultry symbidium of the present invention.

본 발명은 실생종자의 무균종자 발아단계와; 경정절취단계와; 경정배양단계와; 배양된 Mericlone의 3대에 걸친 계대배양 단계로 구성된다.The present invention provides aseptic seed germination step of live seed; Corrective cutting step; Fertility culture step; It consists of three stages of passage of cultured Mericlone.

이하, 본 발명의 구체적인 구성 및 작용을 실시예를 들어 설명하지만, 본 발명의 권리범위가 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.

실시예 1Example 1

Lapine Joyner(♀) × Red Gloria(♂) 계통이 교배된 무균종자 발아 및 생장Germination and Growth of Aseptic Seeds Crossed with Lapine Joyner (♀) × Red Gloria (♂)

심비디움 실생종자를 발아시키기 위하여 Nudson C배지를 선택하고 SO4 -2, Ca+2, NH4 +1이온의 비율을 낮추고 대신 K+1이온의 비율을 높힌 수정배지에서 무균적으로 발아시켜 보통의 방법으로 비배(肥培) 관리하여 생장시켰다.In order to germinate symbiotic seedlings, Nudson C medium was selected and germinated aseptically in fertilized medium with lower ratio of SO 4 -2 , Ca +2 , NH 4 +1 ions and higher ratio of K +1 ions. The embryo was managed by the method to grow.

실생종자는 모본(母本)으로 Lapine Joyner 계통을, 부본(父本)으로 Red Gloria 계통을 교배한 종자를 선택하였다.Seeds were selected to cross the Lapine Joyner line as the parent and the Red Gloria line as the parent.

종피의 살균은 10% Ca(CLO)2용액에 20분간 살균한 후 멸균수에 투입하여 자력교반기를 사용하여 침전된 종자를 사용하였다.Sterilization of the seedlings was performed by disinfection in 10% Ca (CLO) 2 solution for 20 minutes and then put in sterile water and using the seed precipitated using a magnetic stirrer.

실시예 2Example 2

초대 조직배양 배지의 조성Composition of the initial tissue culture badge

상기 실시예 1의 종자배양배지와는 달리 경정(莖頂)의 활착 또는 양란의 초기생육을 촉진하기 위하여 다음과 같이 조성된 배지를 사용하였다.Unlike the seed culture medium of Example 1, in order to promote the initial growth of fertilization or fertilization of fertilization (莖 頂) was used as a medium composition as follows.

성 분ingredient 함 량content KNO3 KNO 3 0.52g/L0.52 g / L CaHPO4 CaHPO 4 0.20g/L0.20 g / L KH2PO4 KH 2 PO 4 0.25g/L0.25g / L (NH4)2SO4 (NH 4 ) 2 SO 4 0.50g/L0.50 g / L MgSO4·7H2OMgSO 4 7 H 2 O 0.25g/L0.25g / L Fe2(C4H4O6)3 Fe 2 (C 4 H 4 O 6 ) 3 0.30g/L0.30 g / L TryptoneTryptone 2g/L2 g / L 서당Sudang 20g/L20 g / L 한천(agar)Agar 7.5g/L7.5g / L

경정적출용 배지는 8g/L의 한천을 첨가해 PLB의 증식에 사용한다. pH는 5.5로 조성하였다.For fertility extraction medium, 8 g / L agar is added to the growth of PLB. pH was set at 5.5.

실시예 3Example 3

원괴체(PLB) 증식Proliferation of Protoplasts (PLB)

원괴체의 증식을 위하여 다음의 KC 배지를 사용하였다.The following KC medium was used for proliferation of protoplasts.

성 분ingredient 함 량content KH2PO4 KH 2 PO 4 0.25g/L0.25g / L Ca(NO3)4H2OCa (NO 3 ) 2 4H 2 O 1.00g/L1.00 g / L (NH4)2SO4 (NH 4 ) 2 SO 4 0.5g/L0.5g / L MgSO4·7H2OMgSO 4 7 H 2 O 0.25g/L0.25g / L FeSO4·7H2OFeSO 4 7H 2 O 0.025g/L0.025 g / L MnSO4·4H2OMnSO 4 4H 2 O 0.0075g/L0.0075 g / L 서당Sudang 20g/L20 g / L 한천(agar)Agar 7.5g/L7.5g / L

실시예 4Example 4

본 발명 신품종 식물체 유도 및 발근Induction and rooting of new plant species

본 발명 신품종 식물체를 유도하고 발근을 위한 배지는 아래 Knudson(1946) 배지를 사용하고 여기서 활성탄을 첨가하여 발근율과 지상 및 지하부 생장을 촉진시켰다.As a medium for inducing and rooting new varieties of the present invention, Knudson (1946) medium below was used, and activated carbon was added to promote rooting rate and ground and underground growth.

성 분ingredient 함 량content KH2PO4 KH 2 PO 4 0.25g/L0.25g / L Ca(NO3)4H2OCa (NO 3 ) 2 4H 2 O 1.00g/L1.00 g / L (NH4)2SO4 (NH 4 ) 2 SO 4 0.5g/L0.5g / L MgSO4·7H2OMgSO 4 7 H 2 O 0.25g/L0.25g / L FeSO4·7H2OFeSO 4 7H 2 O 0.025g/L0.025 g / L MnSO4·4H2OMnSO 4 4H 2 O 0.0075g/L0.0075 g / L ZnSO4·4H2OZnSO 4 4H 2 O 0.0033g/L0.0033 g / L CuSO4 CuSO 4 0.0004g/L0.0004 g / L 서당Sudang 20g/L20 g / L 한천(agar)Agar 7.5g/L7.5g / L

상기 배지조성액들은 100배 액을 만들어 냉장고에 보관하여 사용하되 Ca+2와 SO4 -2, Mg+2와 PO4 -는 불용성 침전물을 형성하므로 이들은 사용시 첨가하였다.The media composition was prepared 100 times the liquid and stored in the refrigerator, but Ca + 2 and SO 4 -2 , Mg +2 and PO 4 - form an insoluble precipitate was added when used.

한편, 원괴체로부터 신초(shoot)가 분화되면 육묘용 300mL Flask에 이식하되 300mL 플라스크에 20주, 500mL 플라스크에 40주를 기준으로 육묘하여 3대에 걸쳐 계대배양하여 품종을 고정하였으며, 본 발명 신품종 "아구리" 식물의 원예적 특성은 다음 표와 같다.On the other hand, when shoots were differentiated from protoplasts, they were transplanted into 300mL Flasks for seedlings, but seedlings were fixed for three generations by seedlings based on 20 weeks in 300mL flasks and 40 weeks in 500mL flasks. The horticultural characteristics of the "Aguri" plant are shown in the following table.

기 관Agency 특 성Characteristics 표현형질Phenotype leaf 잎의 길이(葉長)Leaf length 62㎝ 내외62 cm 잎의 수(葉數)Number of leaves 10∼12장10-12 pieces Flower 꽃의 수Number of flowers 16∼19개16 to 19 꽃대의 길이Length of stalk 50∼80㎝50 to 80 cm 꽃 색(花色)Flower color 복숭아색, 복륜화Peach color 꽃 크기Flower size 가로 3.0㎝, 세로 5.8㎝3.0cm in width, 5.8cm in length 꽃대 기울기Tilting flower 22 개화기(開花期)Flowering period 11∼12월November to December 품종의 용도Use of varieties 분화용Eruption 내병성Disease resistance B급Class B

실시예 5Example 5

신품종 식물의 순화Purification of new varieties of plants

계대배양하여 3개월 정도 경과한 15㎝내외의 유묘는 Flask에 물을 넣어 핀셋으로 배지를 허물고 적취하여 배지를 증류수로 깨끗이 제거한다.Seedlings that are about 15cm old after passage for 3 months are poured into the Flask, and the medium is removed by tweezers, and the medium is removed with distilled water.

순화조건은 자연광의 10% 정도인 5,000∼10,000lux가 되도록 대기습도는 차광하고 통기성이 양호한 바구니에 식재하고 70∼80%를 유지하여 건조되지 않도록 주의하였다.Purification conditions were 10% of natural light, 5,000 ~ 10,000 lux, so that atmospheric humidity was shaded and planted in a good air-permeable basket.

순화된 본 발명 신품종 "아구리" 식물의 개화된 전체의 모습은 사진도 1과 같으며 기존의 양란계통과 현저히 구별되는 복숭아색의 복륜화를 나타내었다.The entire flowering state of the purified new varieties "Aguri" plants is shown in Figure 1, showing a peach-colored abdominal ring that is remarkably distinct from the existing egg system.

이상과 같이 본 발명은 실시예를 통하여 설명한 바와 같이, 본 발명은 복숭아색을 가지는 복륜화로서 분화용의 우수한 양란 심비디움 신품종에 속하는 식물을 제공하는 효과가 있으므로 식물 신품종 육성산업상 매우 유용한 발명인 것이다.As described above, the present invention as described through the embodiment, the present invention is a very useful invention in the plant new breed growth industry because it has the effect of providing a plant belonging to the new breed of Symbidium excellent egg for differentiation as a peach-colored biped.

Claims (1)

기존의 Lapine Joyner(♀) × Red Gloria(♂) 계통의 교배종자를 무균 발아생장시킨 다음 경정(莖頂)을 적출하여 원괴체(PLB)를 증식하고 원괴체로부터 신초(shoot)가 분화되면 이를 Flask에 분주 육묘하여 3대 이상 계대배양에 의하여 무성번식시켜 고정시킴을 특징으로 하는 하기 원예적 특성을 가지는 양란 신비디움에 속하는 "아구리" 식물.Aseptic germination of conventional Lapine Joyner (♀) × Red Gloria (♂) strains is carried out, followed by extraction of the fertilization, proliferation of protoplasts (PLB) and flask when shoots erupt from the protoplasts. An "Aguri" plant belonging to a poultry mysterium having the following horticultural characteristics, characterized in that it is seeded and seeded in three or more subcultures. 1) 잎의 길이 : 62㎝ 내외1) Length of leaves: about 62㎝ 2) 잎의 수 : 10∼12장2) Number of leaves: 10-12 3) 꽃의 수 : 16∼19개3) Number of Flowers: 16 ~ 19 4) 꽃대의 길이 : 50∼80㎝4) Length of stalk: 50∼80㎝ 5) 꽃 색 : 복숭아색, 복륜화5) Flower Color: Peach, Shoe Ring 6) 꽃의 크기 : 가로 3.0㎝, 세로 5.8㎝6) Flower Size: 3.0cm in width, 5.8cm in length 7) 꽃대 기울기 : 27) tilting the stalk: 2 8) 개화기 : 11∼12월8) Blooming season: November to December 9) 품종의 용도 : 분화용9) Use of varieties: for differentiation 10) 내병성 : B급10) Disease resistance: Class B
KR1019990009047A 1999-03-17 1999-03-17 New variety of plant cymbidium Aguri KR100330123B1 (en)

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