KR100330122B1 - New variety of plant cymbidium marky special - Google Patents

Abstract

PURPOSE: A plant Cymbidium Marky special is provided, thereby producing high quality plant Cymbidium Marky special used for differentiation. CONSTITUTION: A plant Cymbidium Marky special is produced by cross-breeding 825-2 with Nana; germinating the cross-breeding plant; extracting the apicalmeristem to produce a protocorm-like body(PLB); differentiating the protocorm-like body(PLB) to produce shoots; transferring the shoots into a flask; and asexual reproducing the shoots by subculturing them above three generations, wherein the plant Cymbidium Marky special has the properties of the leaf length of about 75cm, the leaf number of 10 to 12, the flower number of 11 to 20, the flower stalk length of 55 to 75 cm, the flower color of red, the flower size of 3.3cmx6.5cm, the flower stalk slope of 2 degree, the flowering time of November to January, the use for differentiation, and the disease tolerance of B level.

Description

New breed of plant cymbidium marky special

The present invention relates to a new egg 'Cymbidium' new varieties 'Maqui Special' plants. More specifically, the present invention relates to a plant 'marky special' belonging to a new breed of poultry symbidium, which is fixed as an excellent new breed for differentiation as a red-wheeled shoe.

The origin of the symbidium called the egg eggs is a ring species native to the highlands (800-1,500m) of tropical Southeast Asia, such as northern India, Burma, Thailand and Vietnam. They are bred between the native species or by breeding these species with Japanese and Chinese species.

On the other hand, mass production of symbidium is possible by tissue culture techniques, in particular by the technology of nutrient system, that is, mericlone, which can be obtained by extracting, culturing and growing apical meristem.

In particular, symbidium can be mass-produced by the mericlone technology, and it is easy to cultivate new varieties, and as a result, it can satisfy the demand of consumers by the low price. Accordingly, an object of the present invention is to provide a large amount of new varieties of symbidium which can be satisfied by the consumer at low cost.

The above object of the present invention is to select the existing egg symbidium as the parent and the bundling and hybridization and color (flower), leaf posture (plantation), flowering time (開花 期) aseptic seed species and other new varieties After cultivation by germination, the nutrients, especially spermatozoon, were extracted, tissue cultured, and subcultured.

1 is a photograph showing the phenotype of flowers, leaves, stems, etc. of plants belonging to a new breed of poultry symbidium of the present invention.

The present invention provides aseptic seed germination step of live seed; Corrective cutting step; Fertility culture step; It consists of three stages of passage of cultured Mericlone.

Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.

Example 1

Germination and Growth of Aseptic Seeds Crossed 825-5 (♀) × Nana (♂)

In order to germinate symbiotic seedlings, Knudson C medium was selected and germinated aseptically in fertilized medium with relatively low concentrations of SO ₄ ^-2 , Ca ⁺² , NH ₄ ⁺¹ ions and instead of higher concentrations of K ⁺¹ ions. The embryos were grown in the usual manner and grown.

Live seed was selected from 825-2 (No Name) line as the parent and Nana line as the subordinate.

Sterilization of the seedlings was performed by sterilizing for 20 minutes in a 10% Ca (CLO) 2 solution, and then put into sterile water to use the seed precipitated using a magnetic stirrer.

Example 2

Composition of the initial tissue culture badge

Unlike the seed culture medium of Example 1, in order to promote the initial growth of fertilization or fertilization of fertilization (莖 頂) was used as a medium composition as follows.

For fertility extraction medium, 8 g / L agar is added to the growth of PLB. pH was set at 5.5.

Example 3

Proliferation of Protoplasts (PLB)

The following KC medium was used for proliferation of protoplasts.

Example 4

Induction and rooting of new plant species of the present invention

As a medium for inducing and rooting new varieties of the present invention, Knudson (1946) medium below was used, and activated carbon was added to promote rooting rate and ground and underground growth.

The medium composition was prepared 100 times the liquid and stored in the refrigerator used, but Ca ^{+ 2} and SO ₄ ^-2 , Mg ⁺² and PO ₄ ^- form an insoluble precipitate was added when used.

On the other hand, when shoots were differentiated from protoplasts, they were transplanted into 300mL Flasks for seedlings, but seedlings were fixed for three generations by seedlings based on 20 weeks in 300mL flasks and 40 weeks in 500mL flasks. The horticultural characteristics of the 'Maquisphall' plant are shown in the following table.

1: tilt of flower stand 1: upright 2: about 15 degree bend 3: 30 degree bend 2: disease resistance A: very resistant to root disease B: strong C: normal D: weak E: very weak Example 5

Purification of new varieties of plants

Seedlings that are about 15cm old after 3 months of passage are added to the flask and water is removed by tweezers. The medium is removed with distilled water.

Purified conditions were carefully placed in a basket with good air permeability and light permeability, and kept at 70 to 80% so that the moisture level was 5,000 to 10,000 lux, which is about 10% with nature.

The entire flowering state of the purified new varieties of 'Maquisshall' plants is shown in Figure 1, showing a red abdominal ring that is remarkably distinct from the existing egg production line.

As described above, the present invention, as described through the embodiment, the present invention is a very useful invention in the plant new breed growth industry because it has the effect of providing a plant belonging to the new breed of Symbidium excellent egg for differentiation as a red-wheeled shoes.

Claims (1)

Aseptic germination of existing 825-2 (♀) × Nana (♂) strains is carried out, followed by extraction of the fertilization, proliferation of protoplasts (PLB), and when shoots erupt from the protoplasts, Flask 'Maquissepar' plants belonging to the poultry symbidium having the following horticultural characteristics, characterized in that the seedlings are seeded and seeded by three or more subcultures. 1) Length of leaves: around 75㎝ 2) Number of leaves: 10-12 3) Number of flowers: 11-20 4) Length of stalks: 55∼75㎝ 5) Flower Color: Red, Shoe Ring 6) Flower size: 3.3cm in width, 6.5cm in length 7) tilting the stalk: 2 8) Blooming season: November to January 9) Use of varieties: for differentiation 10) Disease resistance: Class B