KR19990046066A - New variety of plant cymbidium Apple Tea - Google Patents

Abstract

The present invention has an excellent effect of providing a plant "apple tea" belonging to a new breed of excellent egg yolk symbiosis for the differentiation as the abdominal ring having a pink white color.

Description

New egg of Symbidium apple tea plant {New variety of plant cymbidium Apple Tea}

The present invention relates to a new breed of "Cymbidium" "apple tea" plants. More specifically, the present invention relates to a plant "Apple Tea" belonging to a new breed of poultry symbidium which is fixed as an excellent new breed for differentiation as a pinkish white shoe.

The origin of the symbidium called the egg eggs is a ring species native to the highlands (800-1,500m) of tropical Southeast Asia, such as northern India, Burma, Thailand and Vietnam. They are bred between the native species or by breeding these species with Japanese and Chinese species.

On the other hand, mass production of symbidium is possible by tissue culture techniques, in particular by the technology of nutrient system, that is, mericlone, which can be obtained by extracting, culturing and growing apical meristem.

In particular, symbidium can be mass-produced by the mericlone technology, and it is easy to cultivate new varieties, and as a result, it is possible to satisfy consumer demand by low price. Accordingly, an object of the present invention is to provide a large amount of new varieties of symbidium which can be satisfied by the consumer at low cost.

The above object of the present invention is to select the existing egg symbidium as the parent and the bundling and hybridization and color (flower), leaf posture (plantation), flowering time (開花 期) aseptic seed species and other new varieties After cultivation by germination, the nutrients, especially spermatozoon, were extracted, tissue cultured, and subcultured.

1 is a photograph showing the phenotype of flowers, leaves, stems, etc. of plants belonging to a new breed of poultry symbidium of the present invention.

The present invention provides aseptic seed germination step of live seed; Corrective cutting step; Fertility culture step; It consists of three stages of passage of cultured Mericlone.

Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.

Example 1

Germination and Growth of Aseptic Seeds Crossed 902-12 (♀) × Madonna (♂)

In order to germinate symbiotic seedlings, Nudson C medium was selected and germinated aseptically in fertilized medium with lower ratio of SO ₄ ^-2 , Ca ⁺² , NH ₄ ⁺¹ ions and higher ratio of K ⁺¹ ions. The embryo was managed by the method to grow.

Seeds were selected from 902-12 (No Name) lines as the parent and Madonna line as the parent.

Sterilization of the seedlings was performed by disinfection in 10% Ca (CLO) ₂ solution for 20 minutes and then put in sterile water and using the seed precipitated using a magnetic stirrer.

Example 2

Composition of the initial tissue culture badge

Unlike the seed culture medium of Example 1, in order to promote the initial growth of fertilization or fertilization of fertilization (莖 頂) was used as a medium composition as follows.

ingredient content KNO ₃ 0.52 g / L CaHPO ₄ 0.20 g / L KH ₂ PO ₄ 0.25g / L (NH ₄ ) ₂ SO ₄ 0.50 g / L MgSO _{4 7} H ₂ O 0.25g / L Fe ₂ (C ₄ H ₄ O ₆ ) ₃ 0.30 g / L Tryptone 2 g / L Sudang 20 g / L Agar 7.5g / L

For fertility extraction medium, 8 g / L agar is added to the growth of PLB. pH was set at 5.5.

Example 3

Proliferation of Protoplasts (PLB)

The following KC medium was used for proliferation of protoplasts.

ingredient content KH ₂ PO ₄ 0.25g / L Ca (NO ₃ ) ₂ 4H ₂ O 1.00 g / L (NH ₄ ) ₂ SO ₄ 0.5g / L MgSO _{4 7} H ₂ O 0.25g / L FeSO ₄ 7H ₂ O 0.025 g / L MnSO ₄ 4H ₂ O 0.0075 g / L Sudang 20 g / L Agar 7.5g / L

Example 4

Induction and rooting of new plant species

As a medium for inducing and rooting new varieties of the present invention, Knudson (1946) medium below was used, and activated carbon was added to promote rooting rate and ground and underground growth.

ingredient content KH ₂ PO ₄ 0.25g / L Ca (NO ₃ ) ₂ 4H ₂ O 1.00 g / L (NH ₄ ) ₂ SO ₄ 0.5g / L MgSO _{4 7} H ₂ O 0.25g / L FeSO ₄ 7H ₂ O 0.025 g / L MnSO ₄ 4H ₂ O 0.0075 g / L ZnSO ₄ 4H ₂ O 0.0033 g / L CuSO ₄ 0.0004 g / L Sudang 20 g / L Agar 7.5g / L

The media composition was prepared 100 times the liquid and stored in the refrigerator, but Ca ^{+ 2} and SO ₄ ^-2 , Mg ⁺² and PO ₄ ^- form an insoluble precipitate was added when used.

On the other hand, when shoots were differentiated from protoplasts, they were transplanted into 300mL Flasks for seedlings, but seedlings were fixed for three generations by seedlings based on 20 weeks in 300mL flasks and 40 weeks in 500mL flasks. The horticultural characteristics of the "apple tea" plant are shown in the following table.

Agency Characteristics Phenotype leaf Leaf length Around 79㎝ Number of leaves Chapter nine Flower Number of flowers 11-14 Length of stalk 53-56 cm Flower color Pink white, two-wheeled shoes Flower size 1.4cm in width, 6.3cm in length Tilting flower 2 Flowering period 12-March Use of varieties Eruption Disease resistance Class C

Example 5

Purification of new varieties of plants

Seedlings that are about 15cm old after passage for 3 months are poured into the Flask, and the medium is removed by tweezers, and the medium is removed with distilled water.

Purification conditions were 10% of natural light, 5,000 ~ 10,000 lux, so that atmospheric humidity was shaded and planted in a good air-permeable basket.

The entire flowering state of the purified new varieties of "apple tea" plants is shown in Figure 1, showing a pink white abdominal ring that is remarkably distinguished from the existing egg system.

As described above, the present invention, as described through the examples, the present invention is a very useful invention in the plant new breed growth industry because it has the effect of providing a plant belonging to the new breed of Symbidium excellent egg for differentiation as a bicyclic shoe having a pink white.

Claims (1)

Aseptic germination of the existing 902-12 (♀) × Madonna (♂) strains is carried out, followed by extraction of the fertilization and proliferation of protoplasts (PLB), and when shoots are differentiated from the protoplasts, Flask An "apple tea" plant belonging to a poultry mysterium having the following horticultural characteristics, characterized in that it is seeded and seeded in three or more subcultures. 1) Length of leaves: around 79㎝ 2) Number of leaves: 9 3) Number of flowers: 11-14 4) Length of stalk: 53∼56㎝ 5) Flower Color: Pink White, Shoe Ring 6) Flower Size: 1.4cm in width, 6.3cm in length 7) tilting the stalk: 2 8) Blooming season: December ~ March 9) Use of varieties: for differentiation 10) Disease resistance: Class C