KR100532767B1 - Selection of Cymbidium Kanran 'Su-Kwan' new variety - Google Patents
Selection of Cymbidium Kanran 'Su-Kwan' new variety Download PDFInfo
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- KR100532767B1 KR100532767B1 KR10-2003-0078158A KR20030078158A KR100532767B1 KR 100532767 B1 KR100532767 B1 KR 100532767B1 KR 20030078158 A KR20030078158 A KR 20030078158A KR 100532767 B1 KR100532767 B1 KR 100532767B1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/62—Orchidaceae [Orchid family]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
- A01G22/63—Orchids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/12—Leaves
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
본 발명은 단엽종이며 잎의 얼룩이 복륜(覆輪) 형태이고, 초세가 강건하고, 잎의 얼룩모양이 복륜인 우수한 한란 신품종에 속하는 식물 '수관'을 제공하는 뛰어난 효과가 있다.The present invention has an excellent effect of providing a plant 'water tube', which belongs to an excellent new plant species, which is a monocotyledonous leaf, and the leaf spot is the abdominal ring form, the super fineness is strong, and the leaf spot shape is the abdominal ring.
Description
본 발명은 한란(Cymbidium kanran) 신품종 '수관'이라 명명한 식물에 관한 것이다. 더욱 상세하게는, 본 발명은 단엽종이며 잎의 얼룩이 복륜(覆輪) 형태이고, 초세가 강건하고 우수한 신품종으로 고정된 한란 신품종에 속하는 식물 '수관'에 관한 것이다.The present invention relates to a plant named Cymbidium kanran new varieties 'water pipe'. More specifically, the present invention relates to a plant 'water pipe' belonging to a new breed of orchids, which is a monocotyledonous plant, the leaves of which are in the form of abdominal rings, and which are secured with a superb hardy and excellent new species.
한란(寒蘭)으로 부르는 심비디움의 원종(原種)은 대체로 한국, 중국, 일본 등 온대 동남아시아 지방에서 자생하는 지생종(地生種)이며 원예품종은 이들 원종들 간을 교배하거나 이들 원종과 일본종, 중국종과의 교배에 의해 육성된 것들이다.The native species of symbidium called Hanran are mostly native species that grow in temperate Southeast Asia, such as Korea, China, and Japan, and horticultural varieties cross between these species, These were fostered by crosses with Chinese species.
한편, 한란의 대량 생산이 가능한 것은 조직배양(Tissue Culture)기법 특히, 교배된 배(胚)를 무균파종으로 발아하여 배양·증식함으로써 얻을 수 있는 영양계(營養系) 즉, 조직배양 기술을 이용한 영양계 번식의 기술에 의해서이다.On the other hand, it is possible to mass-produce eggs by using tissue culture techniques, in particular, by using nutrient systems, that is, tissue culture techniques, which are obtained by germinating and culturing mated embryos with aseptic seeding. By the technology of nutrient breeding.
특히, 한란은 상기 조직배양 기술을 이용한 영양계 번식 기술에 의해 대량 생산이 가능하고 새로운 품종의 육성이 용이해지고 그 결과 저렴한 가격에 의해 소비자의 수요를 만족할 수가 있다. 따라서, 본 발명의 목적은 수요자가 만족할 수 있는 한란의 신품종을 값싸게 대량 제공함에 있다.In particular, the egg can be mass-produced by the nutrient breeding technology using the tissue culture technology, the breeding of new varieties can be facilitated, and as a result, the demand of the consumers can be satisfied by the low price. Accordingly, an object of the present invention is to provide a large amount of cheap new varieties of eggs that can be satisfied by the consumer.
본 발명의 상기 목적은 기존의 한란 신품종을 무균종자 파종 후 발아된 수많은 근경 중에서 노란색부위 및 노란색 줄무늬 부위에서 그 영양체 선발 계대배양함으로써 달성하였다.The above object of the present invention was achieved by passing nutrient selection screening in the yellow region and the yellow stripe region among the numerous roots germinated after seeding the aseptic seed.
본 발명은 실생종자의 무균종자 발아단계와; 조직배양 기술을 이용한 영양계 번식의 계대배양 단계로 구성된다.The present invention provides aseptic seed germination step of live seed; It consists of passaging stages of nutrient breeding using tissue culture technology.
이하, 본 발명의 구체적인 구성 및 작용을 실시예를 들어 설명하지만, 본 발명의 권리범위가 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific configuration and operation of the present invention will be described with reference to Examples, but the scope of the present invention is not limited only to these Examples.
실시예 1Example 1
자가 교배된 무균종자 발아 및 근경생장Germinated and Root Growth of Self-crossed Sterile Seeds
한란 꽃의 주두(stigma)에서 단단한 노란색의 화분덩어리를 주두 안쪽에 핀셋으로 집어넣고 자가(自家)교배 후 1년 된 완숙 배(胚)를 선택하여 조직배양 무균파종 하였다.조직배양 발아소요기간이 4∼12개월 이내에 종자발아가 빠르고 발아개체수도 많이 발생할 수 있는 종자배양기술을 확립하였다.Tissue culture germ-free seeding was carried out by inserting a solid yellow pollen mass in the stigma of the orchid flower with tweezers inside the ovule, and then ripening pears 1 year old after self-breeding. A seed culture technique was established that could result in rapid seed germination within 4 to 12 months and a high number of germinated individuals.
삭제delete
실생종자를 발아시키기 위하여 하이포넥스(N-P-K=6-7-19) 원예용 4종 복합비료의 무기물 함량을 3g/ℓ하고, 발아촉진제 펩톤(Peptone) 3g/ℓ, 설탕(Sugar) 20g, pH 5.8으로 조정한 후 한천(Agar)이 첨가된 고체 배지를 제조하였으며 무균적으로 발아 생장시켰다.In order to germinate the seed, Hyponex (NPK = 6-7-19) mineral content of the four types of compound fertilizer for horticulture was 3g / l, 3g / l germination promoter peptone, 20g sugar, pH 5.8 After adjusting to Agar (Agar) added a solid medium was prepared and germinated and grown aseptically.
실시예 2Example 2
근경(rhizome)에서 단엽종 얼룩무늬 신초분화Monolithic speckle budding differentiation in rhizome
寒蘭의 경우 급속한 개체 증식을 위해서 근경에서 신초 분화를 위하여 다음의 배지를 사용하였다.In the case of pneumoniae, the following medium was used for differentiation of shoots from myopia for rapid individual proliferation.
한편, 파종하여 발아된 수많은 근경 중에서 노란색부위 및 노란색 줄무늬 부위에서 단엽종 얼룩무늬 신초(shoot)가 분화되는 것을 선발하였으며, 계대배양을 수회 반복하여 다량 생산된 근경에서 신초만 육묘용 500㎖ 배양병에 이식하되 5주를 기준으로 육묘하여 품종을 고정하였으며, 본 발명 신품종 '수관' 식물의 원예적 특성은 다음 표와 같다On the other hand, among the numerous roots germinated and germinated, single leaf speckle shoots were differentiated from yellow and yellow stripes. The cultivars were fixed by transplanting the seedlings based on 5 weeks, and the horticultural characteristics of the new varieties 'water tube' plants of the present invention are shown in the following table.
실시예 3Example 3
신품종 식물의 순화Purification of new varieties of plants
계대배양하여 1년 정도 경과한 벌브경 12mm 내외의 유묘는 배양병에 물을 넣어 핀셋으로 배지를 허물고 적취하여 수돗물로 배지를 깨끗이 제거한다.Seedlings around 12 mm bulb diameter that passed about one year after passage are added to the culture bottle, and the medium is tumbled with tweezers and removed.
순화조건은 통기성이 양호한 바구니에 이식하고 자연광의 10% 정도인 5,000∼10,000lux가 되도록 차광하고 대기습도는 70∼80%를 유지하되 건조되지 않도록 주의하였다.Purifying conditions were transplanted into a basket with good ventilation, and shaded to 5,000 to 10,000 lux, which is about 10% of natural light, and the air humidity was maintained at 70 to 80%.
이상과 같이 본 발명은 실시 예를 통하여 설명한 바와 같이, 본 발명은 단엽종이며 잎의 얼룩이 복륜(覆輪) 형태이고, 초세가 강건하고, 잎의 얼룩모양이 복륜인 우수한 한란 신품종에 속하는 식물을 제공하는 효과가 있으므로 식물 신품종 육성산업상 매우 유용한 발명인 것이다.As described above, the present invention, as described through the embodiment, the present invention is to provide a plant belonging to the excellent Hanran new varieties, which is a single-leafed species, the leaf spot is a double ring form, the super fineness is strong, the leaf spot is a double ring Because it is effective, it is a very useful invention for the new plant breeding industry.
도1은 본 발명의 한란 신품종에 속하는 식물의 잎, 줄기, 표현형질을 나타낸 사진도1 is a photograph showing the leaves, stems, phenotype of plants belonging to a new varieties of Hanran of the present invention
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CN103314862A (en) * | 2013-07-16 | 2013-09-25 | 葛建 | High-efficiency method for obtaining cymbidium detoxified seedling |
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CN106879474A (en) * | 2017-04-21 | 2017-06-23 | 山东省农作物种质资源中心 | A kind of method of restoration ecosystem after cold orchid germ plasm resource Plantlet in vitro and preservation |
CN107079813A (en) * | 2017-04-21 | 2017-08-22 | 山东省农作物种质资源中心 | A kind of method of restoration ecosystem after sword-leaved cymbidium germ plasm resource Plantlet in vitro and preservation |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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KR19990046057A (en) * | 1999-03-17 | 1999-06-25 | 김영우 | New variety of plant cymbidium Mariko |
KR20000056347A (en) * | 1999-02-19 | 2000-09-15 | 이종석 | New cultivar of Cymbidium IL-CHOOL |
KR20030017961A (en) * | 2001-08-25 | 2003-03-04 | 제주도(농업기술원) | Selection of Cymbidium Kanran 'Bohunzo' variety |
KR20030017960A (en) * | 2001-08-25 | 2003-03-04 | 제주도(농업기술원) | Selection of Cymbidium Kanran 'Backlokdam' variety |
KR20030017959A (en) * | 2001-08-25 | 2003-03-04 | 제주도(농업기술원) | Selection of Cymbidium Kanran 'Jeoktoma' variety |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20000056347A (en) * | 1999-02-19 | 2000-09-15 | 이종석 | New cultivar of Cymbidium IL-CHOOL |
KR19990046057A (en) * | 1999-03-17 | 1999-06-25 | 김영우 | New variety of plant cymbidium Mariko |
KR20030017961A (en) * | 2001-08-25 | 2003-03-04 | 제주도(농업기술원) | Selection of Cymbidium Kanran 'Bohunzo' variety |
KR20030017960A (en) * | 2001-08-25 | 2003-03-04 | 제주도(농업기술원) | Selection of Cymbidium Kanran 'Backlokdam' variety |
KR20030017959A (en) * | 2001-08-25 | 2003-03-04 | 제주도(농업기술원) | Selection of Cymbidium Kanran 'Jeoktoma' variety |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103314862A (en) * | 2013-07-16 | 2013-09-25 | 葛建 | High-efficiency method for obtaining cymbidium detoxified seedling |
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