KR100412229B1 - Extraction Method of Polysaccharide from Acanthopanax Fruits - Google Patents

Abstract

The present invention relates to a method of extracting polysaccharides of the fruit of Aggalpi and to a food comprising the extract prepared therefrom. More specifically, (i) the organolpi fruit is kept at a relative humidity of 80-100% and a temperature of 25 ° C.-40 ° C. for dehydration passage. Forming a moisture and drying step including reducing the relative humidity to 5-20% and drying at a temperature of 45 ° C.-60 ° C .; (Ii) pre-treatment step of immersing the dried organolpi fruit in water of 10 ℃-22 ℃ for 3 hours-15 hours; And (iii) an extraction method comprising a high pressure extraction step comprising the step of adding water to the cold-pretreated pre-processed organolpi fruit and a high pressure extraction at 1.0 atm-2.0 atm and a health food comprising an extract prepared therefrom. will be.

Description

Extraction Method of Polysaccharide from Acanthopanax Fruits}

The present invention relates to a method for extracting polysaccharides of the fruit of Aggalpi and a food comprising an extract prepared therefrom, and more particularly to a health food comprising a novel method of extracting polysaccharides from the organolpi fruit in high yield and an extract prepared therefrom. It is about.

As used herein, the term "augalpi (fruit) polysaccharide" is a term that refers to a polysaccharide contained in the genus (Orchidaceae), and its molecular weight is 25,000-500,000, predominantly referred to as a heteroglycan (heteroglycan) (Wagner et al., Drug Res. , 35 (II), Nr. 7: 1069-1075 (1985))

Polysaccharides contained in the plants of the genus Ogalpi are natural substances that have no side effects on the human body, and have been proved to be of great interest because of their excellent biological activity (Woo Wonsik, Natural Products Chemistry Research Method, Minumsa, 194-204 (1984). Belew, Journal of Nursing Midwifery. 44 (3): 232-252 (1999));

The polysaccharides have been reported to exhibit a variety of biological activities, which are as follows: First, the organopolysaccharide was found to have anticancer activity. And increased by 81% (Wang et al., Planta Med. 59: 54-58 (1993); Wang et al., Cancer Letter , 65: 79-84 (1992)). Immunity-enhancing effect was 52% more effective in the treatment group than the control group, and among the 13 plants with superior immunity, the organopolysaccharide was the highest (Wagner et al . , Drug Res. , 35 (II), Nr 7: 1069-1075 ( 1985)). In addition, the effect of phagocytosis in vivo was increased by 68%, and the spleen cell weight was increased by 40% while the number of spleen cells was increased by 26.7% (Wang et al., Planta Med. 57: 335-336 (1991)). ). In addition, the extract containing the organopolysaccharide was drinking to 50 healthy men and women and not only lowered cholesterol LDL by 30% but also reduced triglyceride by 30.1% (Szolomicki et al. Phytother. Res. 14: 30-35 (2000); Deutsche Apotheker zeitung, 130. Jahrg. Nr. 27. May 7: 1499-1508 (1990)). Experiments on the toxic substance resistance and the inhibitory action of Mycobacterium tuberculosis (Shen et al. J. Immunopharmac. , 13 (5): 549-554 (1991)) showed that the resistance to toxic substances in vivo was about 200% higher than the control group. , The number of Mycobacterium tuberculosis was suppressed by about 300%.

As mentioned above, although the organopolysaccharide has been reported to have a pharmacologically important biological activity, it is a source for obtaining the organopoly polysaccharide, more specifically a large amount, in developing a pharmaceutical or food containing the organopolysaccharide. The primary obstacle was to obtain the fruit of the organolpi, and the secondary obstacle was to obtain a fruit with a high polysaccharide content among the organolpi fruit. There was this.

Accordingly, the present inventors have developed a strain of the plant of the algae tree, that is, the koomagapigo tree, which greatly improved the yield of the algae tree fruit by greatly improving the fruiting rate and the fruiting rate as a result of trying to solve the above problems. Filed under application number 2000-11450.

However, until now, no method has been developed for effectively extracting polysaccharides as active ingredients from the organza fruit.

Throughout this specification, numerous patent documents and articles are referred to and their citations are shown in parentheses. The disclosures of cited patents and articles are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the present invention are more clearly explained.

Accordingly, it is an object of the present invention to provide a method for extracting polysaccharides from the organopi fruit.

It is another object of the present invention to provide a polysaccharide extract of the organgalpi fruit.

Another object of the present invention to provide a food comprising a polysaccharide extract of the organgalpi fruit.

According to one embodiment of the present invention, the present invention maintains the organolpi fruit at a relative humidity of 80-100% and a temperature of 25 ℃ -40 ℃ to form a dehydration passage and to reduce the relative humidity to 5-20% and temperature 45 A humid drying step comprising a step of drying at a temperature of about 60 ° C .; And it provides a method of extracting a polysaccharide from the organopi fruit comprising an extraction step.

According to another embodiment of the present invention, the present invention comprises cold pretreatment step of immersing the organolpi fruit in water of 10 ℃-22 ℃ for 3 hours-15 hours; Drying step; And it provides a method for extracting a polysaccharide from the organopi fruit including the extraction step.

According to another embodiment of the present invention, the present invention comprises the steps of drying the organolpi fruit; And it provides a method for extracting a polysaccharide from the organolpi fruit comprising a high-pressure extraction step comprising the step of adding water to the dried organolpi fruit by the drying step and the high pressure extraction at 1.0 atm-2.0 atm.

According to another embodiment of the present invention, the present invention maintains (v) organolpi fruit at a relative humidity of 80-100% and a temperature of 25 ℃ -40 ℃ to form a dehydration passage and to reduce the relative humidity to 5-20% And drying at a temperature of 45 ° C.-60 ° C .; (Ii) pre-treatment step of immersing the dried organolpi fruit in water of 10 ℃-22 ℃ for 3 hours-15 hours; And (iii) provides a method for extracting a polysaccharide from the organopi fruit comprising a high-pressure extraction step comprising the step of adding water to the cold pre-processed organopi fruit and high pressure extraction at 1.0 atm-2.0 atm.

The present invention is described in detail as follows:

Although the method of extracting polysaccharides from the organolpi fruit of the present invention is most preferably applied to the organolpi fruit, it will be apparent to those skilled in the art that it can of course also be applied to other parts (stems, leaves, roots, etc.) of the organolpi tree.

According to the extraction method of the present invention, the problem of the conventional dried dried galvanic fruit method has been greatly improved. According to the conventional method, drying the dried galvanic fruit at room temperature constitutes the mainstream, and even in the case of using a dryer, the temperature under the condition of atmospheric humidity It is used to adjust the drying method. In the conventional drying method, due to the difference in humidity between the inside and the outside of the fruit, the drying is performed first at the outside of the fruit, so that it is difficult to sufficiently elute moisture in the deep portion of the fruit, and thus it is difficult to lower the water content to 14% or less.

However, in the case of the high humidity drying method of the present invention, the moisture content of the fruit can be lowered to 12%, and the fruit cells are decomposed to deep cells to facilitate the extraction of polysaccharides, as well as to increase the shelf life of the final product. It also has the advantage of maintaining uniformity. This advantage is due to the fact that the fruit's external humidity and deep humidity become the same during the maintenance process in the humid conditions, so that a dehydration passage is formed.

In the high humidity drying method of the present invention, when the relative humidity is set to less than 80% in the process of forming the dehydration passage, there is a problem that formation of the dehydration passage is difficult due to a large water difference between the outside of the fruit and the core, and the temperature is less than 25 ° C. When the temperature is low, there is a problem that the drying efficiency is lowered, when the temperature is maintained above 40 ℃ there is a problem that active components are reduced by active metabolism. In addition, when the relative humidity in the drying process is less than 5%, there is an uneconomic problem, if it exceeds 20%, there is a problem that it is difficult to lower the water content to less than 12%, when the drying temperature is less than 45 ℃ drying efficiency is lowered There is a problem that, in excess of 60 ℃ there is a problem that the enzymes in vivo are killed.

More preferably, the high humidity drying method of the present invention is a process of maintaining the relative humidity at 95-99% and the temperature of 30 ° C-35 ° C, and reducing the relative humidity to 8-12% and drying at the temperature of 45 ° C-55 ° C. Most preferably, the process is maintained at a relative humidity of 97-99% and a temperature of 30 ° C.-35 ° C. and a step of slowly decreasing the relative humidity to 10% and drying at a temperature of 50 ° C.

Cold pre-treatment method of the present invention is carried out to facilitate the extraction of the polysaccharide component by infiltrating moisture to the outer layer as well as the core of the organopi fruit. In the cold pre-treatment method of the present invention, when the temperature of the water in which the galvanic fruit is deposited is less than 10 ° C., water does not reach the deep part of the fruit, and when it exceeds 22 ° C., metabolism occurs in the seeds in the fruit. There is a problem that the content of is reduced.

In a more preferred embodiment of the present invention, the cold pre-treatment method is carried out by immersing the organolpi fruit in water of 15 ℃-22 ℃ for 6 hours-12 hours, most preferably in water of 18 ℃-20 ℃ 6 It is carried out by dipping for 9 hours.

On the other hand, as described above, the polysaccharide of the organophyll fruit has a large molecular weight of about 500,000, so that the yield of the polysaccharide is limited under standard pressure. In addition, if the pressure is too high, the sugar bonds of the polysaccharide component are cleaved and decomposed to accelerate the decomposition. Therefore, in order to maximize the extraction of the polysaccharide component of the organopi fruit it is necessary to optimize the pressure used during extraction. The high pressure extraction method of the present invention is devised to meet the above requirements.

In the high pressure extraction method of the present invention, when the pressure is less than 1.0 atm, the pressure is low and the polysaccharide yield is too low. When the pressure is higher than 2.0 atm, glycoside bonds of the polysaccharide are cleaved, and it is difficult to obtain a polysaccharide as a bioactive component. According to a more preferred embodiment of the present invention, the high pressure extraction method is carried out for 30 minutes-70 minutes at 1.2 atm-1.7 atm, most preferably at 1.5 atm for 50 minutes.

Preferred extraction method of the present invention is carried out by combining the cold pretreatment method, the high humidity drying method and the high pressure extraction method. Various combinations can be expected in the form of the combination, but most preferably, all three methods are combined.

The polysaccharide extraction method of the organolpi fruit of the present invention can be applied to a variety of augalpi species, most preferably applied to the fruit of the Komao Galpi tree (Patent Application No. 2000-11450), which is a variety plant developed by the present inventors will be.

The present invention provides a polysaccharide extract of the fruit of the organ galpi prepared by the extraction method of the present invention described above. The extract of the present invention contains various polysaccharides while maintaining bioactivity. Polysaccharides included in the extract of the present invention include polysaccharide PES A, polysaccharide PES B and the like (Deutsche Apotheker zeitung, 130. Jahrg. Nr. 27. 5. 7: 1499-1508 (1990)).

The present invention provides a food comprising the polysaccharide extract of the above-mentioned organgalpi fruit. The food of the present invention may include ingredients that are commonly added during food production. For example, when prepared as a drink, in addition to the polysaccharide extract of the fruit of the gallbladder of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, tofu extract, jujube extract, licorice extract and the like may be further included. The food of the present invention can exert effects such as known bioactive anticancer activity, increased immunity, lowered blood cholesterol, and the like, for the polysaccharides contained in the aforementioned organ blood.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Example I: Comparison of Polysaccharide Extraction of Augalpi Fruits by Drying Methods

Ⅰ-1: Humidity drying method of this invention

The fruit of the organolpi used in this example and the following examples is a fruit obtained from the Komaogalpi varieties plants developed and patented by the present inventor (application number: 2000-11450). The Komao Galpi fruits are generally harvested from late November to mid-December.

First, put 2.5 kg of the Komao galpi fruit in the drying chamber, adjust the relative humidity 97-99% with a nebulizer, and maintain for 48 hours at 30-35 ℃ to dehydrate the fruit to equal the external humidity and the deep humidity of the fruit A passage was formed. The humidity was then slowly reduced to 10% while the temperature was raised to 50 ° C. and dried for 72 hours. Then, 5 liters of distilled water was added to 200 g of the dried fruit, hot water was extracted twice at 100 ° C. for 3 hours in a heating container, and then a large amount of the extracts were extracted by combining the extracts.

Ⅰ-2: Conventional drying method

2.5 kg of the same amount of fruit was put in the same drying chamber used in the above-mentioned high humidity drying method and maintained at a temperature of 30-35 ° C. for 48 hours under the condition of natural atmospheric humidity, and the temperature was raised to 50 ° C. and dried for 72 hours. Then, 5 l of distilled water was added to 200 g of the fruit sample as described in I-1 above, and hot water was extracted twice at 100 ° C. for 3 hours in a heating vessel, and then a large amount of the extract was extracted by combining the extracts.

Ⅰ-3: Measurement of yield of water content and mass flow

Komao galpi fruit used as a sample was dried by two methods of drying and drying by 2.5 kg each of the harvested from the same tree by the method of measuring the weight of the fruit was measured the moisture content of the dried fruit.

The yield measurement of the polysaccharide was carried out as follows: First, the hydrothermal extracts obtained in the above examples were combined and then Whatman No. 4 filter paper, and then concentrated under reduced pressure. Then, 99.5% ethanol was added to the concentrate by diluting 3-fold dilution, and then the precipitate was partitioned and centrifuged. Then, the clarification liquid was discarded, and only the residue was taken and freeze-dried to isolate light brown acicular polysaccharide. The yield of polysaccharide was finally determined by measuring the weight of the isolated crystals. The measurement results are shown in Table 1 below.

division Conventional drying method Humidity Drying Method Function rate 14 ± 0.5% 12 ± 0.5% Yield ^* 100% 108% * Yield is converted to 100 conventional drying method

As can be seen in Table 1, in the case of the humid drying method according to the present invention, by reducing the moisture content than the conventional drying method, not only to facilitate the extraction of polysaccharides to collapse the deep cells of the fruit, but also to increase the shelf life of the final product It has a great effect on maintaining uniformity. Therefore, as shown in Table 1, the yield of the polysaccharide is also increased in the case of the humid drying method of the present invention than the conventional drying method.

Example II Implementation of Cold Pretreatment Method

200 g of dried fruit of Komagapi was immersed in 5 L of distilled water at 18-20 DEG C for 3, 6, 9 and 12 hours. Subsequently, drying was carried out by the conventional method in the same manner as described in Example I-2, and then polysaccharide was extracted in the same manner as in Example I. The yield of the extracted polysaccharide is shown in Table 2 below.

division No treatment 3 hours 6 hours 9 hours 12 hours Yield ^* 100% 105% 114.4% 113.2% 111% * Yield is converted to 100 without treatment

As can be seen in Table 2, the polysaccharide yield of the 6 hours pretreatment test and 9 hours test was 13.2-14.4% higher than that of the no treatment, and the yield tended to decrease in the 12 hours test.

Example III Implementation of High Pressure Extraction Method

200 g of dried dried fruit was dried by a conventional method in the same manner as described in Example I-2, and 5 L of distilled water was added to the dried fruit. Then, high pressure extraction for 50 minutes at the pressure and temperature of Table 3. Then, the amount of polysaccharide was measured and the yield was obtained in the same manner as described in Example I-3. The results are shown in Table 3 below.

division 1.0 atm 1.5 atm 2.0 atm Temperature 100 ℃ 110 ℃ 120 ℃ Yield ^* 100% 112% 108% * Yield is converted to 1.0 atm test port as 100

As can be seen in Table 3, the extraction was the highest yield when the extraction at 1.5 atm pressure, in this case it was found that the temperature of 110 ℃ is the most preferred temperature, the treatment time is 50 minutes is the most preferred time.

Example IV: Most Preferred Extraction Methods of the Invention

By adopting all the methods of Examples I, II, and III described above, polysaccharides were extracted from the organophyllus fruit as follows:

First, 2.5 kg of Koomagapi fruit were put in a drying chamber, and the relative humidity was adjusted to 97-99% with an atomizer, and then maintained at 30-35 ° C. for 48 hours. The humidity was then slowly reduced to 10% while the temperature was raised to 50 ° C. and dried for 72 hours. Then, 5 liters of distilled water was added to 200 g of the dried fruit, pretreated with cold water at 18-20 ° C. for 6 hours, and high pressure extraction was performed at a pressure of 1.5 atm and a temperature of 110 ° C. for 50 minutes. The yield of the extracted polysaccharide is shown in Table 4 below.

division Conventional Extraction Method Invention Extraction Method Yield ^* 100% 124.4% * Yield is converted to 100 by conventional extraction method

Preparation Example 1 Health Food Drink

Purified water was added to the components of the composition of Table 5 to obtain a final volume of 85 ml to prepare a drink containing 150 mg of the polysaccharides of the organo fruit and bark of the bark. Augalpi fruit extract is an extract extracted by the method of Example IV.

division Ogalpi Fruit Extract Bark Golpi Bark Ingredients Tofu Jujube licorice Ingredient ratio 50% 30% 10% 5% 5%

Experimental Example 1 Polysaccharide Content of Fruits by Varieties of Ogalpi

In order to investigate the polysaccharide content of the fruit by the cultivar of the Gallica, the polysaccharide content was calculated in the same manner as in Example IV, and the results are shown in Table 6 below.

division Go away Central Ogalpi Thank you Yield ^* 100% 107% 118% * Yield is converted to 100 ps.

As can be seen in Table 6, when producing a variety of products containing the extract extract, it can be seen that the Komao galpi invented by the present inventors can be a better source of polysaccharides than other conventional varieties.

Experimental Example 2: Polysaccharide Content of Komao Galpi

In order to investigate the polysaccharide content of each part of Koomagapi tree, the extraction was performed in the same manner as in Example IV to calculate the polysaccharide content, and the results are shown in Table 7 below.

division Bark Muscle blood Fruit Yield ^* 100% 121% 184% * Yield is converted to 100 bark

As can be seen in Table 7, the fruit part of the organ parts of the tree is significantly higher in polysaccharide content than the other parts.

As can be seen from the above Experimental Examples I and II, when preparing the agalpi extract of the present invention, Komao galpi is used, and it is most preferable to use the fruit portion.

The present invention provides a method for extracting polysaccharides from the organ galpi. In addition, the present invention provides a polysaccharide extract of the organgalpi fruit. On the other hand, the present invention provides a food comprising a polysaccharide extract of the organgalpi fruit. The extraction method of the present invention is a method that can greatly extract the bioactive polysaccharides from the organolpi fruit in a high yield by greatly improving the conventional method, the food of the present invention containing the extract by this extraction method is a polysaccharide component of the organolpi fruit It is an excellent health food that exerts various effects (anticancer effect, increased immunity, etc.) known for.

Claims (13)

In the method of extracting the polysaccharides from the organolpi fruit including the drying step and the extraction step of the organolpi fruit, The drying step is to maintain the organolpi fruit at a relative humidity of 80-100% and temperature 25 ℃ -40 ℃ to form a dehydration path and to reduce the relative humidity to 5-20% and to dry at 45 ℃ -60 ℃ Method of extracting polysaccharides from the organopi fruit, characterized in that the step of high humidity drying. The method of claim 1, wherein the step of drying the humidity is maintained at a relative humidity of 95-99% and a temperature of 30 ° C-35 ° C to form a dehydration passage, and to reduce the relative humidity to 8-12% and a temperature of 45 ° C-55 ° C. Method for extracting a polysaccharide from the organopi fruit, characterized in that carried out in the process of drying in. In the method of extracting the polysaccharides from the organolpi fruit including the drying step and the extraction step of the organolpi fruit, The method further comprises a cold pretreatment step of immersing the organolpi fruit in water of 10 ° C-22 ° C for 3 hours-15 hours before the drying step. The method of claim 3, wherein the cold pretreatment step of extracting the polysaccharides from the organolpi fruit, characterized in that is carried out by immersing the organolpi fruit in water of 15 ℃-22 ℃ for 6 hours to 12 hours. In the method of extracting the polysaccharides from the organolpi fruit including the drying step and the extraction step of the organolpi fruit, The extracting step is a method for extracting polysaccharides from the organolpi fruit, characterized in that the high-pressure extraction step comprising the step of extracting the high pressure at 1.0 atm-2.0 atm and the process of adding water to the dried organolpi fruit. 6. The method of claim 5, wherein the high pressure extraction step is carried out at 1.2 atm-1.7 atm for 30 minutes-70 minutes. The method of extracting polysaccharides from the organ galpi fruit comprising the following steps: (Iii) cold sedimentation pretreatment step of dipping the organopi fruit in water of 10 ° C.-22 ° C. for 3 hours to 15 hours; (Ii) maintaining the cold treated pre-galvanic fruit at 80-100% relative humidity and 25 ° -40 ° C. to form a dehydration passage and reducing the relative humidity to 5-20% at a temperature of 45 ° C.-60 ° C. A humid drying step comprising a drying step; And (Iii) a high pressure extraction step comprising the step of adding water to the dried organopi fruit and high pressure extraction at 1.0 atm-2.0 atm. 8. The method of claim 7, wherein the cold pretreatment step is carried out by immersing the organolpi fruit in water of 15 ° C-22 ° C for 6 hours-12 hours. The method of claim 7, wherein the step of drying the humidity is maintained at a relative humidity of 95-99% and a temperature of 30 ℃ -35 ℃ to form a dehydration passage and to reduce the relative humidity to 8-12% and a temperature of 45 ℃ -55 ℃ Method for extracting a polysaccharide from the organopi fruit, characterized in that carried out in the process of drying in. 8. The method of claim 7, wherein the high-pressure extraction of the high pressure extraction step is a polysaccharide extract from the organolpi fruit, characterized in that carried out for 30 to 70 minutes at 1.2 atm-1.7 atm. Polysaccharide extract of the fruit of the organgalpi prepared by the method of any one of claims 1 to 9. 11. The polysaccharide extract of the fruit of Ogalpi, according to claim 10, wherein the extract is obtained from the fruit of Koomagapi. delete