KR100385311B1 - Process for preparing ginkgo-tea and ginkgo-tea prepared thereby - Google Patents

Abstract

The present invention is a ginkgo leaf residue (GL) obtained by solvent extraction of the ginkgo biloba leaves with alcohol, ketone, or a mixed solvent thereof and water, and the remaining water layer after re-extracting the solvent extract with a non-polar solvent or a mixed solvent thereof and water ( GF), and optionally a chlorophyll obtained by extracting the tea leaves with a non-polar solvent, and dried, the method for producing ginkgo biloba tea and ginkgo biloba tea produced therefrom, according to the present invention contains a toxic substance of ginkgo biloba Ginkgo biloba tea can be produced without losing its pharmacological effects and the original taste and aroma of ginkgo biloba.

Description

Method for preparing ginkgo biloba tea and ginkgo biloba tea produced by the same {Process for preparing ginkgo-tea and ginkgo-tea prepared thereby}

The present invention relates to the production of ginkgo-tea, which does not contain the toxic substance of ginkgo biloba, but contains its active ingredient as it is. More specifically, the present invention relates to a method for producing ginkgo biloba tea by removing and drying its active ingredient in a water-soluble state on dried ginkgo biloba leaves from which toxic substances have been removed, and the ginkgo biloba tea produced thereby.

The pharmacological components contained in ginkgo biloba are a total of 25 or more flavonoid components and terpenelactone components, bilobalide, and gingkolide A, B, C, M and J. These components promote comprehensive blood flow and are effective in preventing and treating a series of diseases derived from blood flow disorders.

The main natural substance of green tea, which is another plant leaf included in the present invention, is a flavonoid component, and some of them overlap with flavonoids of ginkgo biloba. Therefore, the inclusion of ginkgo biloba leaves in the plant leaves will enhance the flavonoids components will further enhance their efficacy.

Various medicines have been developed by extracting components having a blood flow promoting action from ginkgo biloba and have been marketed under various names. However, natural ginkgo leaves contain unnecessary ingredients in addition to the active pharmacological ingredients. In particular, ginkgo biloba leaves contain ginkgolic acid and the like, which not only have a taste and odor that are not suitable for drinking as tea, but also irritate the skin. Therefore, the removal of these toxic substances contained in ginkgo biloba leaves, like ginkgo pulp, is the most important part of using ginkgo biloba as tea.

In general, the leaves of the plant contain more than 80% moisture, which is constantly maintained in the cell by the expansion, diffusion and osmotic pressure of the plant cells. The expandability is combined with water molecules such as gelatin-like proteins, mucilage, celluloid polymers, starch, etc. of plant cells, for example, -COOH, -OH, -NH ₂ , -CO- It refers to the phenomenon of dissolution, which increases the distance between polymers. Diffusion refers to the nature of the concentration of the substance to reach the equilibrium when the substance concentration in the plant cell membrane is different. However, since plant cell membranes have selectivity, the concentrations of substances inside and outside the cell membranes are different, resulting in osmotic pressure.

When the plant is dried and dehydrated, the plant cells become hygroscopic. At this time, the water content in the vacuole of the plant cells decreases, and the water content present between the polymers decreases, thereby decreasing the size and weight of the plant as the distance between the polymers approaches. Substances in the vacuoles of the dried leaf cells are concentrated heavily to replenish water with strong osmotic pressure upon contact with water. Dried polar polymers, on the other hand, combine with water to form a composite surrounded by water, with water molecules between them, resulting in cell expansion.

This hygroscopicity depends on the method of drying the leaves. For example, when certain leaves are dried at 0 ° C. to room temperature using a vacuum, the physiological function of the plant cells is almost maintained. When the dried leaves are brought into contact with water, they absorb the water and return to the natural leaves before drying. However, in most tea manufacturing processes, the tea leaves are heated and roasted to dry the shade or hot air. In addition, there is an example of fermenting tea leaves as in the case of manufacturing black tea. When heated or fermented, most leaf cells are damaged, and the contact with water reduces the expansion caused by osmotic pressure.

This has described the physiological phenomena between plant leaves and water. Indeed, all water dissolved in water, as well as water, travels through plant cell membranes. The selectivity of the cell membrane limits the movement of unwanted substances into the cells. However, if the leaves are lightly roasted or dried with heat, most of the leaf cells are damaged and their original function is lost. In particular, because the selectivity of the intracellular membrane is damaged, the osmotic phenomenon is damaged. In this case, only the diffusion of the solute and the ability to bind to the dehydrated polymer remain among the expansion factors of the leaves.

Based on the physiological phenomena of plant leaves as described above, the present inventors prepare dried ginkgo biloba leaves from which toxic substances have been removed by various means, and then, by relocating and drying the ginkgo biloba component in a water-soluble state to the cell surface or inside of the ginkgo biloba leaves. Toxic substances of ginkgo biloba are removed and its active ingredients are contained as it is, making a ginkgo biloba composition suitable for drinking tea.

Accordingly, it is an object of the present invention to provide a method for producing ginkgo biloba tea containing its active ingredients without containing toxic substances of ginkgo biloba and ginkgo biloba tea produced thereby.

The present invention obtained by re-extracting the ginkgo leaf residue (GL) and the solvent extract obtained by solvent extraction of the ginkgo biloba leaves with alcohol, ketone, or a mixed solvent of water and water and the solvent extract with a non-polar solvent or a mixed solvent of water and water and evaporated The present invention relates to a method for producing a ginkgo leaf tea by mixing and drying an aqueous layer (GF).

At this time, the chlorophyll obtained by extracting the leaves of Thea sinensis with a non-polar solvent may be further mixed. In this method, hexane, petroleum ether or ligroin can be used as the nonpolar solvent.

In the actual preparation of tea, it is preferable to mix the ginkgo leaf residue GL and the water layer GF at a ratio of 40 to 70:10 to 20 (weight: volume). Moreover, it is preferable to vacuum-dry this mixture at 0 degreeC-room temperature. In the method of adding chlorophyll to tea, the chlorophyll solution in a nonpolar solvent is equal to the solvent distillation rate under agitation at 500-1500 rpm for a mixture of ginkgo biloba residue (GL) and water layer (GF). It is preferable to add at a speed.

The present invention also extracts ginkgo biloba leaves with alcohol, ketone, or a mixed solvent of water and water and dried the remaining residues, and re-extracts the ginkgo leaf residue (GL) and the solvent extract with a non-polar solvent or a mixed solvent of water and evaporates the same. It relates to a ginkgo leaf tea produced by mixing and drying the obtained water layer (GF).

Ginkgo biloba tea according to the present invention may be prepared by further mixing chlorophyll obtained by extracting the tea leaf with a non-polar solvent. Here, the water content of the ginkgo leaf residue (GL) is preferably 5% or less and the water content of the final product of the ginkgo leaf tea is 10% or less.

Hereinafter, the present invention will be described in more detail.

As mentioned above, ginkgo biloba in the natural state contains components other than pharmacological agents that promote blood flow, such as skin irritant ginkgolic acid or the like. Therefore, it is important to remove these toxic substances when using ginkgo biloba as a tea. do. Since these toxic substances are solid substances and their boiling points are high and difficult to remove by distillation, the inventors determined that it would be most preferable to remove them by extraction. In addition, when selecting the ginkgo biloba extract solvent, it is important that the starch and its degradation products in the cell should not be eluted and lost. This is because the dried starch plays an important role in resorption of the active ingredient described later. As a result, it was found that alcohols or ketones such as acetone, methanol, ethanol, or a mixed solvent of them and water were the most suitable extraction solvents in terms of extraction efficiency and price. They elute not only the active ingredient of ginkgo biloba, but also the toxic substance ginkgolic acid and its analogs.

Briefly describing the extraction procedure of the present invention.

Ginkgo biloba is extracted by selecting one of alcohols, ketones, or a mixed solvent of these and water. Ginkgo leaf residue obtained after extraction is added to a vacuum dryer and dried to produce dried Ginkgo leaf residue (GL) having a moisture content of 5% or less and stored. Meanwhile, the solvent extract is reextracted with a nonpolar solvent or a mixed solvent of water and water to remove the zinc glycol acid and its analogue (GH). In this case, as the nonpolar solvent, hexane, petroleum ether, ligroin, and the like, preferably hexane can be used. The remaining water layer after extraction with the nonpolar solvent is the active extract (GF). Dehydrated GL was added to a certain amount of GF, mixed thoroughly, and the mixture was dried to 10% or less of moisture in a vacuum dryer to prepare ginkgo leaf tea.

Specific examples of the present invention are as follows.

Resorption of Ginkgo Biloba Active Ingredients from Ginkgo Leaf Residues (GL)

100 g of ginkgo biloba leaves and 500 ml of 60% acetone are added to a 2000 ml extractor and extracted for 5-10 hours. After filtration, 500 ml of 60% acetone was added to the remaining ginkgo biloba residues and extracted under the same conditions. The remaining ginkgo biloba residue after filtration was dried in a vacuum dryer and dried to be kept at 5% or less of moisture (GL). The weight of GL is in the range of 40 to 70 g.

Acetone is evaporated off from 60% acetone extract in a rotary vacuum distillation unit. The remaining aqueous layer is extracted three times with 400 ml each of hexane. Water is evaporated from the remaining aqueous layer (GF) to 30 ml (GF30).

Extract in the same way to make the final extract 20 mL (GF20), 10 mL (GF10), 5 mL (GF5) and dry matter (GF0). Each GF extract (if GF0 is added again) is mixed with GL obtained in each batch for 20 to 60 minutes, then added to a vacuum dryer and dried to prepare green tea.

1 g of the prepared green tea (dried) was added to 80 ml of water, warmed to 80 ° C, and the turbidity thereof was observed. Among the concentrations of GF, the best turbidity was obtained in GF20 and GF10, and some turbidity was observed in GF30 and GF5. The taste was typical of ginkgo leaf extract (slightly bitter).

Resorption of GF and Chlorophyll on Ginkgo Leaf Residue (GL)

The young leaves of dried Tea Tree ( Thea sinensis ) are extracted with hexane to obtain chlorophyll (TH), which is adsorbed with GL on GL. The purpose of this operation is to make ginkgo biloba tea taste and taste of chlorophyll. In the process of adding chlorophyll and GF to GL, the dispersion state of chlorophyll is very important. The finer the chlorophyll dispersion, the more uniform the chlorophyll adsorption and the better the adsorption of GF. At this time, since water plays a role of promoting mixing, additional water is added when using GF0 (dry). In addition, the presence of a solvent partially dissolved in water is important for the chlorophyll to be uniformly dispersed in water. At this time, the solvent may be used butanol, ethyl acetate, butanone.

Half of TH is dissolved in one of these solvents and then added to a mixture of GL and GF. Important here is the stirring speed of the mixture and the addition speed of TH. For example, a certain amount of GL and GF is added to a stirrer equipped with a vacuum distillation unit and the TH solution is added through the middle of the mixture while stirring at a rotational speed of 500 to 1500 rpm. do. Theoretically, the addition rate of the TH solution is preferably the same as the solvent distillation rate, and generally, the time required to add 50 ml of the TH solution is 5 to 30 minutes. The ginkgo biloba tea produced in this way contains the original active ingredient contained in the ginkgo biloba.

[Experiment 1]

Determination of Active Ingredients-Standards and Test Methods, etc. of the 2nd Amendment, page 1286 <Ginkgo Leaf X>

The average content of ginkgo furabon in 1 g of ginkgo leaf tea was 3.6 mg .

[Experiment 2]

Determination of Jingkolic Acid in Ginkgo Leaf Tea

HPLC methods of Sullivan et al. [Sullivan. J. T .; Richards. C. S .; LIoyd.H.A .; Krishna.G: Planta Med. 44, 175 (1982)] quantified 2-hydroxy-6- (8-pentadecenyl) benzoic acid (ginkgoic acid), which contains the most of ginkgolic acid derivatives in ginkgo biloba leaves. This acid was not detected at all.

EXAMPLE

Hereinafter, the present invention will be described in detail by way of examples, but the scope of the present invention is not limited in any way.

Example 1

100 g of dried ginkgo biloba leaves were taken into a 1000 ml extractor equipped with a stirring device, and 300 ml of 60% acetone was added thereto, followed by extraction at room temperature for 10 hours. After extraction, the solvent extract was separated and stored, and the extract was extracted twice with 300 ml of the same solvent to the ginkgo leaf residue, and the extracts were combined. The remaining ginkgo leaf residue was dried in a vacuum dryer to obtain a ginkgo leaf having a moisture content of 5% or less (GL). At this time, the amount of dried ginkgo leaves (GL) was 40 to 70 g depending on the state of the ginkgo leaves used. It was stored in a moisture-proof container. 60% acetone extract was distilled in a vacuum distillation to completely remove acetone. At this time, the remaining aqueous layer was extracted three times with 100 ml of hexane. Water was evaporated from the remaining aqueous layer (GF) after hexane extraction and 20 ml of concentrate (GF20) was obtained. The hexane dissolved portion was discarded.

Example 2

GF was obtained in the same manner as in Example 1, and water was evaporated to 10 ml (GF10).

Example 3

To the total amount of GL obtained in Example 1, GF20 obtained in Example 1 was added, mixed well, and added to a drier, followed by drying to obtain a ginkgo leaf tea having a water content of 10%. 1 g each was dosed.

Example 4

To the total amount of GL obtained in Example 1, GF10 obtained in Example 2 was added, mixed well, and added to a drier, followed by drying to obtain a tea having a water content of 5% or less. 1 g each was dosed.

Example 5

The dried ginkgo biloba was cut into 30-50 meshes, 100 g of which were placed in a 1000 ml extractor. To this was added 300 ml of methanol and extracted at room temperature for 5-10 hours. After extraction, the resultant was filtered and divided into a ginkgo leaf residue and a methanol extract. The same operation was repeated two more times for the ginkgo leaf residue. The remaining ginkgo leaf residue after filtration was added to a vacuum dryer and dried to obtain a water content of 5% or less (GLm). The methanol extracts were combined and the solvent was evaporated under reduced pressure. 40-50 mL of water was added to this residue, followed by shaking for 30 minutes after adding 100 mL of hexane. After standing for 10 minutes, the aqueous and hexane layers were separated. The same operation was performed twice about the lower water layer. The remaining aqueous layer (GFm) after hexane extraction was concentrated to 20 mL (GFm20). The hexane layer was discarded.

Example 6

GFm was obtained in the same manner as in Example 5, and water was evaporated to 10 ml (GFm10).

Example 7

GFm20 and GLm were mixed and dried to prepare a tea having a water content of 10% or less. 1 g each was dosed.

Example 8

GFm10 and GLm were mixed and dried to prepare tea with a moisture content of 5% or less. One dose was 1 g.

Example 9

Dried ginkgo biloba leaves were cut into 30-50 meshes, 100 g of which were added to a 1000 ml extractor, and 300 ml of ethanol were added, followed by stirring at room temperature for 5 to 10 hours. The ethanol extract was stored by filtration and the same operation was performed twice on the ginkgo leaf residue. Ginkgo leaf residue after ethanol extraction was dried in a vacuum dryer to less than 5% moisture content and stored in a sealed container (GLe). The ethanol extracts were combined and ethanol was removed in a reduced pressure distiller to obtain a residue. After adding 30-50 mL of water to this residue, 200 mL of hexane was added, followed by shaking at room temperature for 30 to 60 minutes, and the hexane layer and the aqueous layer were separated. For the aqueous layer, the same operation was repeated two more times. The aqueous layer was taken and concentrated to 20 mL (GFe20) and the hexane layer was discarded.

The entire procedure of the above example was repeated twice to obtain an aqueous layer and concentrated to obtain 10 ml (GFe10) and dried product (GFe0), respectively.

Example 10

After mixing GFe20 and GLe, tossed and dried under reduced pressure, a tea having a water content of 10% or less was prepared. One dose was 1 g.

Example 11

After mixing GFe10 and GLe, tossed and dried under reduced pressure, a tea having a water content of 5% or less was prepared. One dose was 1 g.

Example 12

After drying the young leaves of the tea tree, 100 g of these were added to the extractor. To this was added 300 ml of hexane and stirred at room temperature for 1-3 hours. After filtering, the hexane extract was stored and the same operation was repeated twice more for the remaining plants. The hexane layers were combined and concentrated to give a green residue. Half of the obtained residue was dissolved in 50 ml of ethyl acetate (TH50).

50 g of GLm (or GLe), GFm0 (or GFe0) and 50 to 70 ml of distilled water are added to a stirrer equipped with a 500 ml vacuum distillation unit, for example a rotary vacuum distiller, at a speed of 500 to 1000 rpm. Rotated. A small amount of TH50 was flown through the solvent injection tube to the midpoint of the mixture. When the flow rate was about 5 ml per minute, it was adjusted to evaporate about 5 ml per minute of ethyl acetate. The addition time was 10-20 minutes. When the addition of TH50 was completed, water was evaporated to prepare a dry dry matter. The dried product was dried under reduced pressure to make tea with 10% or less moisture. 1 g each was dosed.

According to the present invention can be produced without toxic substances of ginkgo biloba leaves ginkgo biloba tea can enjoy its pharmacological effects and the unique taste and aroma of ginkgo biloba as it is.

Claims (9)

Ginkgo biloba leaves obtained by solvent extraction for 5 to 10 hours with alcohol, ketone, or a mixed solvent of water and water and the remaining residues dried to below 5% water content (Gl) and the solvent extract to hexane, petroleum ether and ligroin The water layer (GF) obtained by re-extracting and evaporating with a nonpolar solvent selected from the group consisting of or a mixed solvent thereof and water is mixed at a ratio of 40 to 70: 10 to 20 (weight: volume), and dried in vacuo at 0 ° C to room temperature to make ginkgo leaves. How to make tea. The chlorophyll obtained by extracting the Thea sinensis leaves with a nonpolar solvent selected from the group consisting of hexane, petroleum ether and ligroin for 1 to 3 hours is further added with Ginkgo leaf residue (GL) and aqueous layer (GF) ( In case of GF0, water is added), and the mixture is mixed at the same rate as the solvent distillation rate under stirring at 500-1500 rpm. delete delete delete delete Ginkgo biloba tea, which is prepared according to the method of claim 1. 8. Ginkgo leaf tea according to claim 7, characterized in that it is prepared according to the method of claim 2. The ginkgo leaf tea according to claim 7 or 8, wherein the ginkgo leaf residue (GL) has a moisture content of 5% or less and the ginkgo leaf tea has a moisture content of 10% or less.