KR950016771A - Purification Method of Ginkgo Biloba Bioactive Substance - Google Patents

Purification Method of Ginkgo Biloba Bioactive Substance Download PDF

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Publication number
KR950016771A
KR950016771A KR1019930030200A KR930030200A KR950016771A KR 950016771 A KR950016771 A KR 950016771A KR 1019930030200 A KR1019930030200 A KR 1019930030200A KR 930030200 A KR930030200 A KR 930030200A KR 950016771 A KR950016771 A KR 950016771A
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South Korea
Prior art keywords
polar solvent
ginkgo biloba
solvent
water
physiologically active
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KR1019930030200A
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Korean (ko)
Inventor
안병구
최외순
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하기주
주식회사 코오롱
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Priority to KR1019930030200A priority Critical patent/KR950016771A/en
Publication of KR950016771A publication Critical patent/KR950016771A/en

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Abstract

본 발명은 은행잎 생리활성물질들을 보다 간단하고 안정하게 추출하는 방법에 관한 것으로서, 은행잎 생리활성물질을 물, 극성용매 및 상기 극성용매보다 상대적으로 극성이 낮은 용매로 이루어진 혼합용매중에서 용해도차이에 의해 추출함을 특징으로하며, 이러한 본발명에 의하면 무기염류첨가, 용액의 PH조절등의 과정을 배제하고도 간단하고 경제적인 방법으로 높은 순도를 유지하면서 효과적으로 은행잎 생리활성물질을 정제 추출할 수 있게 된다.The present invention relates to a method for more simple and stable extraction of ginkgo biloba bioactive substances, wherein the ginkgo biloba bioactive substance is extracted by a difference in solubility in water, a polar solvent and a mixed solvent consisting of a solvent having a relatively lower polarity than the polar solvent. According to the present invention, it is possible to effectively extract and extract ginkgo biloba bioactive substances while maintaining high purity in a simple and economical manner, excluding the addition of inorganic salts and pH control of the solution.

Description

은행잎 생리활성물질의 정제추출방법Purification Method of Ginkgo Biloba Bioactive Substance

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따라 실시한 실시예 1에서 제조된 은행잎 생리 활성성분을 지표물질인 퀘세틴, 캄페톨, 이소탐네틴에 대해 환산된 정량분석 고성능 액테크로마토그래피,1 is a quantitative analysis of high-performance ectochromatography converted from the physiologically active ingredient of ginkgo biloba prepared in Example 1 according to the present invention for quercetin, camphortol, and istamnetine,

제2도는 종전의 방법에 따른 비교예에서 제조된 은행잎 생리활성 성분의 정량분석 고성능 액체크로마토그래피,2 is a quantitative analysis of high performance liquid chromatography of ginkgo biloba bioactive components prepared in Comparative Example according to the conventional method,

제3도는 표준물질인 퀘세틴과 캄페톨의 고성능 액테크로마토 그라피.Figure 3 is a high performance actectomy chromatography of quercetin and camphortol as standard.

Claims (5)

은행잎으로부터 생리활성물질을 정제추출함에 있어서, 건조분쇄한 30∼50메쉬의 은행잎 분말을 저급알콜과 저급케톤, 물등의 혼합용매로 추출하고 비극성용매로 은행잎의 클로로필을 제거한 뒤 용액을 감압농축시켜 얻어진 잔사를 물/극성용매/중간극성용매의 부피비가 1/0.1∼0.6/0.5∼1.5로 조성된 혼합 용매로 용해시키고, 상온에서 방치하여 유기충과 물층으로 충분리시키고, 이때 얻어진 유기층을 감압농축하고, 얻어진 잔사를 에탄올에 용해시키고 여과한뒤 농축 및 건조시켜 은행잎으로부터 생리활성성분을 얻는 것을 특징으로 하는 은행잎 생리활성물질의 정제추출방법.In purifying physiologically active substances from ginkgo biloba, dry crushed 30-50 mesh ginkgo biloba powder was extracted with a mixed solvent such as lower alcohol, lower ketone, water, etc. The residue was dissolved in a mixed solvent having a volume ratio of water / polar solvent / intermediate polar solvent of 1 / 0.1 to 0.6 / 0.5 to 1.5, left at room temperature to suffice the organic layer and the water layer, and the organic layer was concentrated under reduced pressure. , The obtained residue is dissolved in ethanol, filtered and concentrated and dried to obtain a physiologically active component from the ginkgo biloba leaves, characterized in that the purified extraction method of physiologically active ginkgo leaves. 제1항에 있어서, 물/극성용매/중간극성용매의 부피비가 1/0.2∼0.4/0.9∼1.2인 것을 특징으로 하는 은행잎의 생리활성물질의 정제추출방법.The method of claim 1, wherein the water / polar solvent / medium polar solvent has a volume ratio of 1 / 0.2 to 0.4 / 0.9 to 1.2. 제1항에 있어서, 비극성용매는 노르말헥산, 석유에테르, 사염화탄소인 것을 특징으로 하는 은행잎 생리활성물질의 정제추출방법.The method of claim 1, wherein the non-polar solvent is normal hexane, petroleum ether, carbon tetrachloride. 제1항에 있어서, 극성용매는 메탄올, 에탄올, 아세톤, 아세토니트릴에서 선택됨을 특징으로 하는 은행잎 생리활성물질의 정제추출방법.The method of claim 1, wherein the polar solvent is selected from methanol, ethanol, acetone, acetonitrile. 제1항에 있어서, 중간극성용매는 이소프로판올, 노르말부탄올, 이소부탄온, 펜탄올, 리틸에틸케톤, 디에틸케톤, 벤젠, 톨루엔, 에틸아세테이트 중에서 선택됨을 특징으로 하는 은행잎 생리활성물질의 정제추출방법 .The method of claim 1, wherein the mesopolar solvent is selected from isopropanol, normal butanol, isobutanone, pentanol, ritriethyl ketone, diethyl ketone, benzene, toluene, and ethyl acetate. . ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930030200A 1993-12-28 1993-12-28 Purification Method of Ginkgo Biloba Bioactive Substance KR950016771A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100385311B1 (en) * 2000-10-11 2003-05-23 박화목 Process for preparing ginkgo-tea and ginkgo-tea prepared thereby

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100385311B1 (en) * 2000-10-11 2003-05-23 박화목 Process for preparing ginkgo-tea and ginkgo-tea prepared thereby

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