KR0138542B1 - The fomentation method using high-temperature fermentation microorganism - Google Patents

Abstract

The present invention relates to a method of steaming using high temperature fermentation microorganisms. More specifically, the present invention relates to a method of steaming the human body using metabolism of heat and electric microorganisms generated by high-temperature fermentation microorganisms. The mixed culture of the high temperature fermentation microorganism in the present invention is about 2 to 3% by volume relative to the fermentation medium containing 2 to 80% by weight of agricultural by-products, honey trace amount, 20 to 98% by weight of water can be used as a carbon source Inoculated as possible, and left for about 16 to 30 hours when the temperature of the culture solution was raised to 60 ℃ or more was used as a medium for poultice. By replacing the fermentation medium used in the steaming of the present invention with fresh fermentation medium of 2 to 4% by weight daily, continuous use was possible. The method of steaming according to the present invention has an effect on skin waste, sweat and toxin decomposition, blood circulation, promotion of physiological activity, stress relief, skin softening and regeneration, removal of spots, diabetes, renal failure and blood pressure control.

Description

Steaming method using high temperature fermentation microorganism

The present invention relates to a method of steaming using high temperature fermentation microorganisms. More specifically, the present invention relates to a method of steaming the human body using metabolism of heat and electric microorganisms generated by high-temperature fermentation microorganisms.

In general, the poultice may be divided into a moist heat method and a dry heat method. Representative methods of moist heat include a method of exposing the whole body for a predetermined time using a warm bath and steam soaking the whole body in hot water for a predetermined time. In addition, typical methods using dry heat include a method of steaming a local part of the body using an electric device that generates heat, and a method of steaming by exposing a part of the body using infrared rays. Of the two methods, moist heat steaming is the preferred method of folk remedies, most of the cases to steam the whole body.

In general, it is known that the systemic treatment by moist heat is effective in relieving stress, improving metabolism, muscle relaxation, sweat release, toxin release, and eliminating spots. It is known to have effects such as muscle relaxation and softening of joints. Such a method of steaming is widely used in physical therapy, and can be said to be an easy way to relieve the fatigue accumulated in everyday life as well as the treatment of diseases.

Expecting various physiotherapy effects by simply soaking the whole body in hot water is the most popular method of steaming. In order to increase the effect of poultice, other substances (such as bioactive substances or skin cosmetics) may be added to hot water. For example, systemic immersion baths that use bran to alleviate dermatological disease, increase tension between nerves and skin, relieve skin itching, release scales and dead skin cells. It is effective against skin diseases such as elimination, itching and skin excitability, poison ivy, prickly heat and eczema, and these therapeutic effects are effective in calming and stimulating the skin's excitement in bran. It is known to be due to a softening component (Min Kyung-ok, Heat & Hydrotherapy, 250-251 (1985), University College).

However, in the case of the whole body immersion bath using the bran mentioned in the foregoing, there is a problem such as not using the fermentation microorganism as in the present invention, of course, energy consumption for maintaining 37 to 40 ℃ at the temperature of the preferred hot water And its effect was limited.

The inventors of the present invention utilize the metabolism of heat and electric microorganisms generated by high-temperature fermentation microorganisms without supplying energy from the outside, to decompose skin wastes, sweat and toxins, promote blood circulation, promote physiological activity, relieve stress, To find out how to soften and regenerate the skin, remove blemishes, diabetes, renal failure and blood pressure control, and to complete the present invention.

After all, the main object of the present invention is to provide a steaming method using the metabolism of heat and electric microorganisms generated by high-temperature fermentation microorganisms.

Hereinafter, the present invention will be described in more detail.

In order to separate the high-temperature fermentation microorganisms from the natural world in accordance with the object of the present invention, the sample is suspended in sterile distilled water during fermentation in a natural state such as compost in the home, and then serially diluted to nutrient agar, Plated in potato dextrose agar and yeast malt-extract agar plate medium and incubated at 40-65 ° C. for 3 days. Bacteria isolated from colonies formed on plate media are used as seed for seed culture and main culture. First, the seed is cultivated in the seed culture medium, and gradually cultured by increasing the culture solution, and then inoculating the electroculture solution to the fermentation medium containing the agricultural by-product and honey or sugar which can be used as a carbon source. The composition of the seed culture medium was 5 g / l peptone, 3 g / l yeast extract, 3 g / l malt extract and 10 g / l glucose; The composition of the culture medium is 5 to 10 g / l degreasing steel powder, trace amount of honey, 0.02 to 0.08 g / l peptone, 0.02 to 0.08 g / l yeast extract and 0.02 to 0.08 g / l malt extract. In the present invention, the term "fermentation medium" is a term introduced to indicate the physical properties of the dough state is not a complete liquid or a complete solid of the material used in the steaming.

The cultured high-temperature fermentation microorganism culture medium is 2 to 80% by weight, more preferably 30 to 70% by weight, most preferably 40 to 60% by weight of the agricultural and by-products that can be used as a carbon source and a trace amount of honey or sugar And fermentation medium containing 20 to 98% by weight of water, more preferably 30 to 79% by weight, most preferably 40 to 60% by weight of water. That is, after inoculating the culture solution of the high-temperature fermentation microorganism in the container containing the fermentation medium enough to the human body to be about 2 to 3% by volume of the total fermentation medium, the temperature of the culture solution is left for about 16 to 30 hours. If it rises above 60 ℃, use it as a medium for steaming. In addition, the agricultural by-products in the present invention is preferably a substance that can be used as a carbon source, such as skim steel, salt, sawdust, rice bran, barley bran or bran, more preferably skim steel, salt or It is appropriate to use sawdust. On the other hand, the fermentation medium can be used continuously by replacing with 2 to 4% by weight of fresh fermentation medium every day.

In the present invention, the container used as a fermentation tank for steaming, preferably uses a size of 2m to 2m, 30cm, 1m to 1m 50cm in height and 50cm to 70cm in height, can be appropriately selected according to the size of the human body . In the fermentation tank, the human body is preferably steamed in a state of lying down as possible, and the steaming time is appropriate for 20 to 30 minutes, but may be appropriately added or subtracted depending on the state of health.

As the high temperature fermentation microorganism in the present invention, yeast, Bacillus genus, Lactobacillus genus, Streptococcus genus and actinomycetes may be used, and a mixed culture of these microorganisms is preferably used.

The steaming method using the high temperature fermentation microorganism of the present invention, compared with other well-known method of moist heat steaming, skin waste, sweat and toxin decomposition, blood circulation, stimulation of physiological activity, stress relief, skin softening and regeneration promotion, removal of spots, diabetes Excellent for renal failure and blood pressure control.

Hereinafter, the present invention will be described in detail by examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples.

Example 1

Pure separation of yeast

Composts from the maturity are collected and suspended in sterile distilled water, followed by serial dilution to plate nutrient agar, potato dextrose agar and yeast malt-extract agar. The plate was incubated for 3 days at 65 ° C. Then, a colony formed on the plate medium was secured, and the above process was repeated twice to select one of the high-temperature fermenting microorganisms having a large colony and excellent growth at 65 ° C. as the best dominant species. Selected strains were partially identified as yeast based on colony color, morphology, size and oxidation / fermentation test (see P. Gerhardt et al., Manual of Methods for General Bacteriology, 420). 443 (1981), American Society of Microbiology.

Example 2

Cultivation of yeast

Yeast, the best dominant species in Example 1, was aseptically inoculated into a test tube containing 5 ml of sterile species culture medium, and cultured for 12 hours at a temperature of 30 ° C. and 180 rpm in a rotory shaker. The composition of the medium is 5 g / l peptone, 3 g / l yeast extract, 3 g / l malt extract and 10 g / l glucose. After taking 1 ml of the culture medium, the cells were inoculated into a 500 ml flask containing 100 ml of sterilized seed culture medium and incubated for 12 hours in a rotary shaker at 30 ° C. and 180 rpm. Then, 50 ml of the culture solution was taken, and then inoculated into a 10 L jar fermentor containing 8 L of sterilized main culture medium, followed by incubation for 12 hours while feeding at a culture pH of 7.0, a culture temperature of 30 ° C., a stirring speed of 200 rpm, and an oxygen supply amount of 1 vvm. It was used as the inoculum for fermentation of the fermentation medium. The composition of the main culture medium is 8 g / l skim steel powder, 0.03 g / l honey, 0.05 g / l peptone, 0.03 g / l yeast extract and 0.03 g / l malt extract.

Example 3

Fermentation of Fermentation Medium Using Yeast

The culture medium prepared in Example 2 is 8-12 L in a container having a width of 2 m 20 cm, 1 m, 30 cm, 60 cm high with fermentation medium containing 200 to 240 kg of skim steel or ink or sawdust, about 250 kg of honey or sugar and 160 to 200 kg of water. And incubated. As a result, the temperature rose to 45 ° C. after about 12 hours, and reached almost 60 ° C. after about 20 hours, and the temperature rose to about 65 ° C. after about 30 hours. When the fermentation medium subjected to the electric fermentation was removed by about 8 to 16kg daily, and the same amount of fermentation medium was added, the temperature could be maintained continuously.

Example 4

Pure separation of high temperature fermentation microorganism

Part of the fermentation medium in Example 3 during the fermentation was taken, suspended in sterile distilled water, and then serially diluted to nutrient agar, potato dextrose agar and yeast malt-extract agar ( yeast malt-extract agar) was plated on a plate medium and incubated for 3 days at 40 ℃. Then, the colonies (colony) formed on the plate medium was secured, and the above-described process was repeated twice to select high-fermentation microorganisms having a large colony and excellent growth at a culture temperature of 40 ° C as dominant species. Each of the selected dominant species was partially identified based on the color, shape, size, and oxidation / fermentation test of the colony (see P. Gerhardt et al., Manual of Methods for General Bacteriology, 420). 443 (1981), American Society of Microbiology. As a result, in addition to one yeast in Example 1, two species of Bacillus (Bacillus), one species of Lactobacillus (Lactobacillus) and one species of Streptococcus (Steptococcus) and three actinomycetes were identified. Therefore, it was found that various microorganisms were involved in addition to the yeast of Example 1 in the actual fermentation process of the fermentation medium.

Example 5

Mixed culture of high temperature fermentation microorganism

The high-temperature fermentation microorganisms separated in Example 4 were sterilely inoculated into a test tube containing 5 ml of sterile seed culture medium, and mixed culture was carried out for 12 hours at 30 ° C. and 180 rpm in a rotary shaker. The composition of the culture medium is 5 g / l peptone, 3 g / l yeast extract, 3 g / l malt extract and 10 g / l glucose. After 1 ml of the culture was inoculated into a 500 ml flask containing 100 ml of sterile cultivation medium and incubated for 12 hours in a rotary vibrator at 30 ° C. and 180 rpm. Then, 50 ml of the culture solution was taken, and then inoculated into a 10 L jar fermentor containing 8 L of sterilized main culture medium. The culture medium was composed of 8 g / l skim, 0.03 g / l honey, 0.05 g / l peptone, 0.03 g / l yeast extract and 0.03 g / l malt extract.

Example 6

Fermentation medium of fermentation medium using mixed culture of high temperature fermentation microorganism

The mixed culture solution prepared in Example 5 was cultured by adding 8 to 12L to the same container as in Example 3 containing fermentation medium of skimmed steel, ink or sawdust 160 to 200 kg, about 250 kg of honey and 200 to 240 kg of water. As a result, the temperature rose to 45 ° C. after about 8 hours, reached almost 60 ° C. after about 16 hours, and the temperature rose to about 65 ° C. after about 26 hours. When the fermentation medium having undergone the electric fermentation was removed about 8 to 16 kg daily and the same amount of fresh fermentation medium was added, this temperature could be maintained continuously.

Therefore, when inoculating the fermentation medium with yeast alone as in Example 3, the inoculation of the mixed culture medium of the high-temperature fermentation microorganisms reduced the overall running time of the fermentation by about 4 hours, the mixture of the electric high-temperature fermentation microorganisms in the fermentation medium Inoculation of the culture solution has been found to be desirable.

Example 7

Mixed culture fermentation with different composition of fermentation medium

As a result of incubating the mixed culture solution prepared in Example 5 in the same container as in Example 3 containing fermentation medium of rice bran, barley bran or wheat bran 240 to 280 kg, about 250 kg of sugar and 120 to 160 kg of water, The results were almost similar to 6. Therefore, it was confirmed that agricultural by-products that can be used as carbon sources such as rice bran, barley bran, or bran can be used as fermentation medium instead of degreasing steel, jelly or sawdust.

Example 8

Effect test by the poultice method of the present invention

In order to test the effect of the steaming method of the present invention, a total of 50 volunteers seeking diversion were placed in containers containing fermentation mediums A, B, and C prepared in Examples 3, 6, and 7, respectively. Then, by steaming for about 30 minutes, the poultice effect was investigated sensually (see Table 1).

[Table 1] Poultice effect according to the poultice method of the present invention

As shown in Table 1 above, when steaming by the method of steaming of the present invention, the mood is refreshed and the body and spirit become poor by the decomposition of skin waste and the discharge of sweat, and the skin becomes soft with the release of stress. I could see that. In addition, it can be seen that the fermentation mediums A, B, and C prepared in Examples 3, 6, and 7 have almost no difference from each other.

As described and demonstrated in detail above, the present invention provides a method for steaming the human body using heat and their metabolism generated by high-temperature fermentation microorganisms. The method of steaming according to the present invention has an effect on skin waste, sweat and toxin decomposition, blood circulation, promoting physiological activity, relieving stress, promoting skin softening and regeneration, removing blemishes, diabetes, renal failure and blood pressure control.

Claims (3)

In a fermentation medium consisting of 2 to 80% by weight of agricultural by-products, 20 to 98% by weight of honey or sugar and water to 2 to 3% by volume of the total fermentation medium, Adding, and heating the human body by using the metabolism of heat and electric microorganisms generated by high-temperature fermentation microorganisms by leaving it for about 16 to 30 hours. The method according to claim 1, wherein the agricultural by-products are at least one substance selected from the group consisting of skimmed steel, jelly, sawdust, rice bran, barley bran, and bran. The method of claim 1, wherein the high temperature fermentation microorganism is at least one microorganism selected from the group consisting of yeast, Bacillus genus, Lactobacillus genus, Streptococcus genus and actinomycetes.