CN108174747A - A kind of preparation method of birds, beasts and eggs Cordyceps militaris - Google Patents
A kind of preparation method of birds, beasts and eggs Cordyceps militaris Download PDFInfo
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- CN108174747A CN108174747A CN201810074277.7A CN201810074277A CN108174747A CN 108174747 A CN108174747 A CN 108174747A CN 201810074277 A CN201810074277 A CN 201810074277A CN 108174747 A CN108174747 A CN 108174747A
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- eggs
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- cordyceps militaris
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Abstract
The invention discloses a kind of preparation methods of birds, beasts and eggs Cordyceps militaris.The product mainly using fresh birds, beasts and eggs, by simply processing, accesses Cordyceps militaris spawn, cultivates fruiting bodies of cordyceps militaris.Party's forensic science, easy to operate, raw material is easy to get, environmentally safe.The birds, beasts and eggs Cordyceps militaris cultivated not only had been rich in the aminoacid ingredient of birds, beasts and eggs, but also rich in cordycepin, cordycepic acid and adenosine and other functional ingredient in Cordyceps militaris.With improving immunity, alleviating the different physiological roles edible safeties such as body fatigue, beautifying face and moistering lotion, without any side effects, it is suitble to promote and apply.
Description
Technical field
The invention belongs to field of functional food, and in particular to a kind of preparation method of birds, beasts and eggs Cordyceps militaris.
Background technology
Cordyceps militaris Cordyceps militaris L.Link also known as northern Chinese caterpillar Fungus, Cordceps militaris, pupa grass, pupa grass bacterium etc.,
Belong to Ascomycota Ascomyeota, Hypocreales Hypocreales, cordyceps sinensis Cordycepps Cordycipitaceae, Cordyceps
Cordyceps.The phorozoon of Cordyceps militaris draws up mould Paecilomyces militaris for pupa.The host range packet of Cordyceps militaris
Include pupa, adult or the larva of the insects such as Lepidoptera, coleoptera and Diptera.Cordyceps militaris have it is similar with cordyceps sinensis it is active into
Point and pharmacological action, and artificial culture technique is ripe, has been increasingly becoming the ideal substitute of cordyceps sinensis.
Cordyceps militaris is one of important artificial substituting product of cordyceps sinensis, in reducing blood lipid, prevents artery sclerosis, the protection heart,
Brain tissue, tranquilizing soporific enhance macrophage activity, and anticancer, anti-inflammatory, antibacterial, anti anoxia etc. have good effects.It is domestic
Analysis comparison is carried out to Cordyceps militaris and cordyceps sinensis chemical composition, the two ingredient is quite similar, cordyceps sinensis it is main effectively into
It is divided into：Cordycepin, cordycepic acid, a sterols, ucleosides, Cordyceps sinensis polysaccharide, SOD enzymes etc..Pharmaceutical research show these effectively into
Divide and played an important role to medical-therapeutic treatment of human body, health care etc.
Not yet there are birds, beasts and eggs and the product of Cordyceps militaris combination at present.
Invention content
Birds, beasts and eggs and Cordyceps militaris are effectively combined the object of the present invention is to provide a kind of, it is easier to absorption of human body, utmostly
Ground retains effective active constituent, a kind of preparation method of the high birds, beasts and eggs Cordyceps militaris product of nutritive value.
The purpose of the present invention is what is be accomplished by the following way：
A kind of preparation method of birds, beasts and eggs Cordyceps militaris, this method are prepared by the following method to obtain：
1) by fresh birds, beasts and eggs a concentration of 75% alcohol solution dipping 1-2 hours, surface is cleaned with sterile material, using going out
Bacterium draw point drills through small sircle hole on birds, beasts and eggs top, and Cordyceps militaris strain is injected into along the aperture drilled through in birds, beasts and eggs, is adjusted
Injection position in birds, beasts and eggs causes strain multiple spot in birds, beasts and eggs to be distributed.
2) birds, beasts and eggs after injection strain are placed in culture bottle, keep vaccination upward, and cover the bottle with ventilated membrane
Lid；
3) culture bottle equipped with birds, beasts and eggs after inoculation is subjected to dark culturing.
The cultivation temperature of above-mentioned dark culturing is 18-20 DEG C, humidity 60%-80%, is cultivated 30-35 days.It is preferred that dark training
The foster time is 35 days.
Above-mentioned Cordyceps militaris strain is injected into birds, beasts and eggs, and each birds, beasts and eggs are injected 3-5 times, and each 1-2ml preferably injects 3-
4 times, each 1ml.The injection position of the adjustment in birds, beasts and eggs is mobile syringe needle, respectively the upper, middle and lower of egg into
Row injection.
The commercially available purchase of cordyceps of use obtains, and can also be provided by Chinese Academy of Sciences's Culture Collection.
It is preferred that use strain of the Cordyceps militaris strain for the small intensive suspension of bacterium ball.
Cordyceps militaris strain can also be prepared in the following way:PDA enrichment incubation based formulas：Potato (peeling)
200g, glucose 20g, peptone 5g, agar 18g, water 1000mL, pH are natural.It is prepared as follows：Take fresh peeled potatoes
200g is cut into 1cm fritters, and water 800mL is added to boil, until potato is thoroughly well cooked but not mushy, through 4 layers of filtered through gauze, 18g fine jades are added in into filtrate
Fat continues to heat, and is stirred continuously until that agar dissolves completely, adds in glucose and peptone stirs evenly, last warm water is extremely
1000mL is distributed into test tube while hot, and every is filled to test tube 1/3 and highly locates, and is wrapped with 7 for a bundle and is put into high-pressure sterilizing pot
In, after the completion of sterilizing, inclined-plane is put into after being cooled to 60 DEG C by 121 DEG C of sterilizing 30min, is subject to liquid level from test tube plug 2cm, solidification
After be put into 28 DEG C of incubator 1d, continue to employ after observing no varied bacteria growing.
It takes out in soya bean size Cordyceps militaris fungus block access blank cuvette, is inoculated with 20 altogether, test tube is protected from light in 25 DEG C of incubators
Culture to mycelia covers with 4/5 inclined-plane.
The present invention has the following advantages compared with other products：
(1) this product is birds, beasts and eggs and the product of Cordyceps militaris combination.Double effects with the two, and it is more easy to absorption of human body.
(2) birds, beasts and eggs are rich in protein, and good protein content is very high, by taking egg as an example.Contain protein per hectogram egg
12.8 grams, predominantly ovalbumin and ovoglobulin, wherein containing 8 kinds of amino acid needed by human, and with the group of human body protein
Poling is approximation, and human body may be up to 98% to the absorptivity of egg protein.It is main to collect per fatty 11~15 grams of hectogram egg
In in yolk, be also easily digested, in yolk containing abundant lecithin, steroid, lecithin and calcium,
Phosphorus, iron, vitamin A, vitamin D and B family vitamin.These ingredients are of great advantage to the function of promoting nervous system, therefore, chicken
Egg is preferable brain-care foods again.
Heat contained by one egg is equivalent to the heat of half of apple or half cup milk, but it also possesses 8%
Phosphorus, 4% zinc, 4% iron, 12.6% protein, 6% vitamin D, 3% vitamin E, 6% vitamin A, 2%
Vitamin B, 5% vitamin B2,4% vitamin B6.These nutrition are all that human body is essential, they are played extremely
Important role, such as repair tissue, form new tissue, consumption energy and participate in complicated metabolic processes.
(3) this product is integrally fermented for Poultry and Eggs, is not required to filter after fermentation, is also not required to as other culture mediums (such as rice
Culture medium) removal culture medium.The birds, beasts and eggs active ingredient in product is remained well；
(4) after this product culture, by being simply dried, so that it may which direct marketing is edible.In process of production,
Without stages such as boilings, reduce the variations such as protein denaturation, farthest retain effective active constituent；
(5) present invention uses specific liquid spawn preparation process, can improve functional component-adenosine in egg Cordyceps militaris
With the content of polysaccharide.
(6) this product is convenient, tasty, is suitble to promote and apply.
Specific embodiment
It is further illustrated the present invention below by way of specific embodiment.But the detail of embodiment is only used for explaining this hair
It is bright, it should not be construed as limited overall technical solution.
Embodiment 1：
It is prepared by 1.1 Cordyceps militaris strains:
Cordyceps militaris spawn is provided by Chinese Academy of Sciences's Culture Collection, and through Chinese Academy of Sciences's microbe research
It is identified.PDA enrichment incubation based formulas：Potato (peeling) 200g, glucose 20g, peptone 5g, agar 18g, water 1000mL,
PH is natural.It is specific as follows：Fresh peeled potatoes 200g is taken, is cut into 1cm fritters, water 800mL is added to boil, until potato is thoroughly well cooked but not mushy,
Through 4 layers of filtered through gauze.18g agar is added in into filtrate, continues to heat, is stirred continuously until that agar dissolves completely.Add in grape
Sugar and peptone stir evenly, and last warm water to 1000mL is distributed into test tube while hot, and every is filled to test tube 1/3 and highly locates.With 7
Branch is wrapped for a bundle and is put into high-pressure sterilizing pot, 121 DEG C of sterilizing 30min.After the completion of sterilizing, inclined-plane is put into after being cooled to 60 DEG C,
Liquid level be subject to from test tube plug 2cm.28 DEG C of incubator 1d are put into after solidification, are continued to employ after observing no varied bacteria growing.
Between being first inoculated with and superclean bench is through ultraviolet disinfection 30min, and personnel change after isolation takes to enter and be inoculated with.Both hands are used
After 75% ethanol disinfection, Cordyceps militaris parent species test tube plug is opened by alcolhol burner flame, takes out the big small bacteria block access blank examination of soya bean
Guan Zhong is inoculated with 20 altogether.Test tube is protected from light culture in 25 DEG C of incubators and covers with 4/5 inclined-plane to mycelia, is placed in 4 DEG C of refrigerators and preserves, protects
The phase of depositing can reach 6 months.
1.2 get out Fresh Egg, culture bottle, draw point, disposable 1ml asepsis injectors.
For Fresh Egg in advance with 75% alcohol solution dipping 1 hour, taking-up before inoculation cleans surface with sterilizing filter paper.
Culture bottle and draw point etc. shift to an earlier date sterilization treatment.
Egg and each operation utensil are placed under ultraviolet irradiation before operation and sterilize 30min.
1.3 drill through small sircle hole using draw point on egg top；
1.4 draw Cordyceps militaris strain using disposable sterilized injector, and along the above-mentioned aperture drilled through, bacterium solution is noted
It injects in egg.Total co-injection 3 times, each 1ml adjusts the injection position in egg and strain multiple spot in egg is distributed
(mainly moving syringe needle, injected respectively in the upper, middle and lower of egg).
1.5 are placed in the egg after injection strain in culture bottle, keep vaccination upward, and cover the bottle with ventilated membrane
Lid.
Culture bottle equipped with egg after inoculation is placed in growth cabinet and carries out dark culturing by 1.6, cultivation temperature 18-20
DEG C, humidity 60%-80%.Cultivation cycle about 35 days.
Embodiment 2：
It is prepared by 1.1 Cordyceps militaris strains:
Cordyceps militaris spawn is provided by Chinese Academy of Sciences's Culture Collection, and through Chinese Academy of Sciences's microbe research
It is identified.PDA enrichment incubation based formulas：Potato (peeling) 200g, glucose 20g, peptone 5g, agar 18g, water 1000mL,
PH is natural.It is as follows：Take fresh peeled potatoes 200g, be cut into 1cm fritters, water 800mL is added to boil, until potato it is ripe without
It is rotten, through 4 layers of filtered through gauze.18g agar is added in into filtrate, continues to heat, is stirred continuously until that agar dissolves completely.Add in Portugal
Grape sugar and peptone stir evenly, and last warm water to 1000mL is distributed into test tube while hot, and every is filled to test tube 1/3 and highly locates, with
It wraps for 7 and is put into high-pressure sterilizing pot for a bundle, 121 DEG C of sterilizing 30min.After the completion of sterilizing, inclined-plane is put into after being cooled to 60 DEG C,
Liquid level be subject to from test tube plug 2cm.28 DEG C of incubator 1d are put into after solidification, are continued to employ after observing no varied bacteria growing.
Between being first inoculated with and superclean bench is through ultraviolet disinfection 30min, and personnel change after isolation takes to enter and be inoculated with.Both hands are used
After 75% ethanol disinfection, Cordyceps militaris parent species test tube plug is opened by alcolhol burner flame, takes out the big small bacteria block access blank examination of soya bean
Guan Zhong is inoculated with 20 altogether.Test tube is protected from light culture in 25 DEG C of incubators and covers with 4/5 inclined-plane to mycelia, is placed in 4 DEG C of refrigerators and preserves.
1.2 get out Fresh Egg, culture bottle, draw point, disposable 1ml asepsis injectors.
For Fresh Egg in advance with 75% alcohol solution dipping 2 hours, taking-up before inoculation cleans surface with sterilizing filter paper.
Culture bottle and draw point etc. shift to an earlier date sterilization treatment.
Egg and each operation utensil are placed under ultraviolet irradiation before operation and sterilize 30min.
1.3 drill through small sircle hole using draw point on egg top；
1.4 draw Cordyceps militaris strain using disposable sterilized injector, and along the above-mentioned aperture drilled through, bacterium solution is noted
It injects in egg.Total co-injection 3 times, each 1ml adjusts the injection position in egg and strain multiple spot in egg is distributed
(mainly moving syringe needle, injected respectively in the upper, middle and lower of egg).
1.5 are placed in the egg after injection strain in culture bottle, keep vaccination upward, and cover the bottle with ventilated membrane
Lid.
Culture bottle equipped with egg after inoculation is placed in growth cabinet and carries out dark culturing by 1.6, cultivation temperature 18-20
DEG C, humidity 60%-80%, cultivation cycle about 32 days.
Embodiment 3：
It is prepared by 1.1 Cordyceps militaris strains:
Cordyceps militaris spawn is provided by Chinese Academy of Sciences's Culture Collection, and through Chinese Academy of Sciences's microbe research
It is identified.PDA enrichment incubation based formulas：Potato (peeling) 200g, glucose 20g, peptone 5g, agar 18g, water 1000mL,
PH is natural.It is as follows：Take fresh peeled potatoes 200g, be cut into 1cm fritters, water 800mL is added to boil, until potato it is ripe without
It is rotten, through 4 layers of filtered through gauze, 18g agar is added in into filtrate, continues to heat, is stirred continuously until that agar dissolves completely, add in Portugal
Grape sugar and peptone stir evenly, and last warm water to 1000mL is distributed into test tube while hot, and every is filled to test tube 1/3 and highly locates, with
It wrapping for 7 and is put into high-pressure sterilizing pot for a bundle, after the completion of sterilizing, inclined-plane is put into after being cooled to 60 DEG C by 121 DEG C of sterilizing 30min,
Liquid level be subject to from test tube plug 2cm, 28 DEG C of incubator 1d are put into after solidification, are continued to employ after observing no varied bacteria growing.
Between being first inoculated with and superclean bench is through ultraviolet disinfection 30min, and personnel change after isolation takes to enter and be inoculated with.Both hands are used
After 75% ethanol disinfection, test tube plug is opened by alcolhol burner flame, takes out in the big small bacteria block access blank cuvette of soya bean, is inoculated with altogether
20, test tube is protected from light culture in 25 DEG C of incubators and covers with 4/5 inclined-plane to mycelia, is placed in 4 DEG C of refrigerators and preserves, storage life can reach
6 months.
1.2 get out fresh duck egg, culture bottle, draw point, disposable 1ml asepsis injectors.
For fresh duck egg in advance with 75% alcohol solution dipping 2 hours, taking-up before inoculation cleans surface with sterilizing filter paper.Training
It supports bottle and draw point etc. and shifts to an earlier date sterilization treatment.Duck's egg and each operation utensil are placed under ultraviolet irradiation before operation and sterilize 30min.
1.3 drill through small sircle hole using draw point on duck's egg top；
1.4 draw Cordyceps militaris bacterium solution using disposable sterilized injector, and along the above-mentioned aperture drilled through, bacterium solution is injected into
In duck's egg.Total co-injection 5 times, each 1ml adjusts the injection position in duck's egg and strain multiple spot in duck's egg is distributed, main
If mobile syringe needle, is injected respectively in the upper, middle and lower of egg.
1.5 are placed in the duck's egg after injection strain in culture bottle, keep vaccination upward, and cover the bottle with ventilated membrane
Lid.
Culture bottle equipped with duck's egg after inoculation is placed in growth cabinet and carries out dark culturing by 1.6, cultivation temperature 18-20
DEG C, humidity 60%-80%.Cultivation cycle about 35 days.
The quality measurements for the product being prepared according to embodiment method see the table below：
Claims (6)
1. a kind of preparation method of birds, beasts and eggs Cordyceps militaris, it is characterised in that this method is prepared by the following method to obtain：
1) by fresh birds, beasts and eggs a concentration of 75% alcohol solution dipping 1-2 hours, surface is cleaned with sterile material, utilizes the steel that sterilizes
Needle drills through small sircle hole on birds, beasts and eggs top, and Cordyceps militaris strain is injected into along the aperture drilled through in birds, beasts and eggs, is adjusted in birds, beasts and eggs
In injection position cause strain in birds, beasts and eggs multiple spot be distributed.
2) birds, beasts and eggs after injection strain are placed in culture bottle, keep vaccination upward, and cover the bottle cap with ventilated membrane；
3) culture bottle equipped with birds, beasts and eggs after inoculation is subjected to dark culturing.
2. the preparation method of birds, beasts and eggs Cordyceps militaris according to claim 1, it is characterised in that the culture of the dark culturing
Temperature is 18-20 DEG C, humidity 60%-80%, is cultivated 30-35 days.
3. the preparation method of birds, beasts and eggs Cordyceps militaris according to claim 1, it is characterised in that the time of the dark culturing
It is 35 days.
4. the preparation method of birds, beasts and eggs Cordyceps militaris according to claim 1, it is characterised in that the Cordyceps militaris bacterium solution injection
Enter in birds, beasts and eggs, each birds, beasts and eggs are injected 3-5 times, each 1-2ml.
5. the preparation method of birds, beasts and eggs Cordyceps militaris according to claim 1, it is characterised in that the adjustment is in birds, beasts and eggs
It is mobile syringe needle to inject position, is injected respectively in the upper, middle and lower of egg.
6. the preparation method of birds, beasts and eggs Cordyceps militaris according to claim 1, it is characterised in that the Cordyceps militaris strain
Strain for the small intensive suspension of bacterium ball.
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| CN201810074277.7A CN108174747A (en) | 2018-01-25 | 2018-01-25 | A kind of preparation method of birds, beasts and eggs Cordyceps militaris |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110295117A (en) * | 2019-07-26 | 2019-10-01 | 周口职业技术学院 | The culture and preparation method of a kind of Cordceps militaris in egg |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1626647A (en) * | 2003-12-12 | 2005-06-15 | 张天宝 | Chinese caterpillar fungus and producing method |
| CN1935978A (en) * | 2006-10-16 | 2007-03-28 | 曾树生 | Method for culturing Chinese caterpillar domestic fungus from fresh egg |
| CN101113415A (en) * | 2007-07-08 | 2008-01-30 | 曾建军 | Method for solid culture of Cordyceps sinensis edible mushroom |
| CN102726208A (en) * | 2011-04-14 | 2012-10-17 | 李永安 | Egg-sprouted worm grass |
| CN104823714A (en) * | 2015-05-04 | 2015-08-12 | 聂晶石 | Large-scale cultivation method for cordyceps militaris |
-
2018
- 2018-01-25 CN CN201810074277.7A patent/CN108174747A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1626647A (en) * | 2003-12-12 | 2005-06-15 | 张天宝 | Chinese caterpillar fungus and producing method |
| CN1935978A (en) * | 2006-10-16 | 2007-03-28 | 曾树生 | Method for culturing Chinese caterpillar domestic fungus from fresh egg |
| CN101113415A (en) * | 2007-07-08 | 2008-01-30 | 曾建军 | Method for solid culture of Cordyceps sinensis edible mushroom |
| CN102726208A (en) * | 2011-04-14 | 2012-10-17 | 李永安 | Egg-sprouted worm grass |
| CN104823714A (en) * | 2015-05-04 | 2015-08-12 | 聂晶石 | Large-scale cultivation method for cordyceps militaris |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110295117A (en) * | 2019-07-26 | 2019-10-01 | 周口职业技术学院 | The culture and preparation method of a kind of Cordceps militaris in egg |
| CN110295117B (en) * | 2019-07-26 | 2021-05-28 | 周口职业技术学院 | Method for culturing and preparing cordyceps militaris in eggs |
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