JPWO2022145354A1 - - Google Patents
Info
- Publication number
- JPWO2022145354A1 JPWO2022145354A1 JP2022573049A JP2022573049A JPWO2022145354A1 JP WO2022145354 A1 JPWO2022145354 A1 JP WO2022145354A1 JP 2022573049 A JP2022573049 A JP 2022573049A JP 2022573049 A JP2022573049 A JP 2022573049A JP WO2022145354 A1 JPWO2022145354 A1 JP WO2022145354A1
- Authority
- JP
- Japan
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020219481 | 2020-12-28 | ||
| PCT/JP2021/048095 WO2022145354A1 (ja) | 2020-12-28 | 2021-12-24 | 標的核酸断片の検出方法及びキット |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPWO2022145354A1 true JPWO2022145354A1 (https=) | 2022-07-07 |
Family
ID=82259424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022573049A Pending JPWO2022145354A1 (https=) | 2020-12-28 | 2021-12-24 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20240094199A1 (https=) |
| EP (1) | EP4269581B1 (https=) |
| JP (1) | JPWO2022145354A1 (https=) |
| WO (1) | WO2022145354A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114072520A (zh) * | 2019-07-04 | 2022-02-18 | 国立研究开发法人理化学研究所 | 检测靶核酸片段的方法及试剂盒 |
| JPWO2023195542A1 (https=) * | 2022-04-08 | 2023-10-12 | ||
| JP2025033452A (ja) * | 2023-08-29 | 2025-03-13 | 国立研究開発法人産業技術総合研究所 | マルチプレックス核酸検出のためのセンサアレイ |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015115635A1 (ja) * | 2014-01-31 | 2015-08-06 | 凸版印刷株式会社 | 生体分子解析キット及び生体分子解析方法 |
| US20170362644A1 (en) * | 2016-06-16 | 2017-12-21 | The Regents Of The University Of California | Methods and compositions for detecting a target rna |
| WO2019098301A1 (ja) * | 2017-11-17 | 2019-05-23 | 凸版印刷株式会社 | 標的分子の検出方法 |
| WO2019104058A1 (en) * | 2017-11-22 | 2019-05-31 | The Regents Of The University Of California | Type v crispr/cas effector proteins for cleaving ssdnas and detecting target dnas |
| JP7579577B2 (ja) * | 2019-07-04 | 2024-11-08 | 国立研究開発法人理化学研究所 | 標的核酸断片の検出方法及びキット |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3727026B2 (ja) | 2003-04-10 | 2005-12-14 | 博行 野地 | 一分子酵素活性検出に用いられるマイクロチャンバと１０００ｆＬ以下の液滴を調製する方法 |
| US20140004539A1 (en) * | 2012-06-15 | 2014-01-02 | The Regents Of The University Of Michigan | Systems and methods for multiplex solution assays |
| CA3119971A1 (en) * | 2018-11-14 | 2020-05-22 | Pardis SABETI | Multiplexing highly evolving viral variants with sherlock |
| WO2022133108A2 (en) * | 2020-12-17 | 2022-06-23 | Mammoth Biosciences, Inc. | Methods and compositions for performing a detection assay |
-
2021
- 2021-12-24 US US18/269,264 patent/US20240094199A1/en active Pending
- 2021-12-24 WO PCT/JP2021/048095 patent/WO2022145354A1/ja not_active Ceased
- 2021-12-24 JP JP2022573049A patent/JPWO2022145354A1/ja active Pending
- 2021-12-24 EP EP21915214.7A patent/EP4269581B1/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015115635A1 (ja) * | 2014-01-31 | 2015-08-06 | 凸版印刷株式会社 | 生体分子解析キット及び生体分子解析方法 |
| US20170362644A1 (en) * | 2016-06-16 | 2017-12-21 | The Regents Of The University Of California | Methods and compositions for detecting a target rna |
| WO2019098301A1 (ja) * | 2017-11-17 | 2019-05-23 | 凸版印刷株式会社 | 標的分子の検出方法 |
| WO2019104058A1 (en) * | 2017-11-22 | 2019-05-31 | The Regents Of The University Of California | Type v crispr/cas effector proteins for cleaving ssdnas and detecting target dnas |
| JP7579577B2 (ja) * | 2019-07-04 | 2024-11-08 | 国立研究開発法人理化学研究所 | 標的核酸断片の検出方法及びキット |
Non-Patent Citations (4)
| Title |
|---|
| COMMUN. BIOL., vol. 4, 476, p.1-7, Supplementary Information, JPN6022010527, 19 April 2021 (2021-04-19), ISSN: 0005743388 * |
| NAT. COMMUN., vol. 5, 4519, p.1-8, JPN6022010525, 2014, ISSN: 0005743386 * |
| SCIENCE, vol. vol.356, p.438-442, JPN6022010526, 2017, ISSN: 0005743387 * |
| バイオサイエンスとインダストリー, vol. vol.79, no.5, p.427-429, JPN6022010528, 10 September 2021 (2021-09-10), ISSN: 0005743389 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4269581B1 (en) | 2025-06-11 |
| EP4269581C0 (en) | 2025-06-11 |
| US20240094199A1 (en) | 2024-03-21 |
| EP4269581A4 (en) | 2024-10-09 |
| WO2022145354A1 (ja) | 2022-07-07 |
| EP4269581A1 (en) | 2023-11-01 |
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