JPWO2021209601A5 - - Google Patents
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- Publication number
- JPWO2021209601A5 JPWO2021209601A5 JP2022543191A JP2022543191A JPWO2021209601A5 JP WO2021209601 A5 JPWO2021209601 A5 JP WO2021209601A5 JP 2022543191 A JP2022543191 A JP 2022543191A JP 2022543191 A JP2022543191 A JP 2022543191A JP WO2021209601 A5 JPWO2021209601 A5 JP WO2021209601A5
- Authority
- JP
- Japan
- Prior art keywords
- anion exchanger
- salt
- wash
- range
- salt solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 11
- 150000001450 anions Chemical class 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 150000003839 salts Chemical class 0.000 description 9
- 239000012266 salt solution Substances 0.000 description 8
- 239000000356 contaminant Substances 0.000 description 7
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 239000006167 equilibration buffer Substances 0.000 description 2
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- MBYLVOKEDDQJDY-UHFFFAOYSA-N tris(2-aminoethyl)amine Chemical compound NCCN(CCN)CCN MBYLVOKEDDQJDY-UHFFFAOYSA-N 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 150000002357 guanidines Chemical class 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 125000001302 tertiary amino group Chemical group 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20170184.4 | 2020-04-17 | ||
| EP20170184.4A EP3896159A1 (fr) | 2020-04-17 | 2020-04-17 | Procédé de purification d'arn simple brin utilisant un échangeur d'anions |
| PCT/EP2021/059911 WO2021209601A1 (fr) | 2020-04-17 | 2021-04-16 | Procédé de purification d'arn simple brin utilisant un échangeur d'anions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023522526A JP2023522526A (ja) | 2023-05-31 |
| JPWO2021209601A5 true JPWO2021209601A5 (fr) | 2024-04-26 |
Family
ID=70482246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022543191A Pending JP2023522526A (ja) | 2020-04-17 | 2021-04-16 | アニオン交換体を用いる一本鎖ｒｎａ精製方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20230142167A1 (fr) |
| EP (1) | EP3896159A1 (fr) |
| JP (1) | JP2023522526A (fr) |
| CN (1) | CN115087736A (fr) |
| WO (1) | WO2021209601A1 (fr) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7626017B2 (en) * | 1997-10-31 | 2009-12-01 | Pressure Biosciences, Inc. | Pressure-enhanced extraction and purification |
| WO2009100172A1 (fr) * | 2008-02-07 | 2009-08-13 | Ge Healthcare Bio-Sciences Corp. | Isolation de l'adn, de l'arn et de protéine à partir d'un seul et même échantillon |
| CN103993002A (zh) * | 2013-02-19 | 2014-08-20 | 百奥迈科生物技术有限公司 | 一种大规模合成长链rna药物的生产新工艺 |
| EP2983804A4 (fr) | 2013-03-15 | 2017-03-01 | Moderna Therapeutics, Inc. | Purification d'arnm par échange d'ions |
| MX2018012880A (es) * | 2016-04-22 | 2019-03-28 | Biontech Rna Pharmaceuticals Gmbh | Metodos para proporcionar arn de hebra sencilla. |
| WO2018096179A1 (fr) * | 2016-11-28 | 2018-05-31 | Curevac Ag | Procédé de purification d'arn |
-
2020
- 2020-04-17 EP EP20170184.4A patent/EP3896159A1/fr active Pending
-
2021
- 2021-04-16 WO PCT/EP2021/059911 patent/WO2021209601A1/fr active Application Filing
- 2021-04-16 JP JP2022543191A patent/JP2023522526A/ja active Pending
- 2021-04-16 CN CN202180013502.6A patent/CN115087736A/zh active Pending
- 2021-04-16 US US17/918,242 patent/US20230142167A1/en active Pending
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