JPWO2021174004A5 - - Google Patents
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- JPWO2021174004A5 JPWO2021174004A5 JP2022552125A JP2022552125A JPWO2021174004A5 JP WO2021174004 A5 JPWO2021174004 A5 JP WO2021174004A5 JP 2022552125 A JP2022552125 A JP 2022552125A JP 2022552125 A JP2022552125 A JP 2022552125A JP WO2021174004 A5 JPWO2021174004 A5 JP WO2021174004A5
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- Prior art keywords
- cells
- concentration
- cell
- induction medium
- bulk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 68
- 210000004027 cell Anatomy 0.000 claims 52
- 239000002609 medium Substances 0.000 claims 25
- 230000006698 induction Effects 0.000 claims 22
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 16
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims 11
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 claims 10
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 claims 10
- 238000012258 culturing Methods 0.000 claims 9
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 9
- 102100020715 Fms-related tyrosine kinase 3 ligand protein Human genes 0.000 claims 8
- 101710162577 Fms-related tyrosine kinase 3 ligand protein Proteins 0.000 claims 8
- 102000036693 Thrombopoietin Human genes 0.000 claims 8
- 108010041111 Thrombopoietin Proteins 0.000 claims 8
- 229960005070 ascorbic acid Drugs 0.000 claims 8
- 235000010323 ascorbic acid Nutrition 0.000 claims 8
- 239000011668 ascorbic acid Substances 0.000 claims 8
- 108010002586 Interleukin-7 Proteins 0.000 claims 7
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 6
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 6
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 claims 6
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 6
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 6
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 6
- 150000001875 compounds Chemical class 0.000 claims 5
- 210000000822 natural killer cell Anatomy 0.000 claims 5
- 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 claims 4
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 claims 4
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 claims 4
- 108010002350 Interleukin-2 Proteins 0.000 claims 4
- 239000012826 P38 inhibitor Substances 0.000 claims 4
- 102100021669 Stromal cell-derived factor 1 Human genes 0.000 claims 4
- 101710088580 Stromal cell-derived factor 1 Proteins 0.000 claims 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 4
- 229910052760 oxygen Inorganic materials 0.000 claims 4
- 239000001301 oxygen Substances 0.000 claims 4
- 238000012174 single-cell RNA sequencing Methods 0.000 claims 4
- 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 claims 3
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 claims 3
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 claims 3
- 210000002865 immune cell Anatomy 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 239000012190 activator Substances 0.000 claims 2
- 201000011510 cancer Diseases 0.000 claims 2
- 239000006143 cell culture medium Substances 0.000 claims 2
- 239000003814 drug Substances 0.000 claims 2
- 238000000684 flow cytometry Methods 0.000 claims 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 claims 1
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 claims 1
- 102000000704 Interleukin-7 Human genes 0.000 claims 1
- 206010025323 Lymphomas Diseases 0.000 claims 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
- 102100030742 Transforming growth factor beta-1 proprotein Human genes 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 238000002659 cell therapy Methods 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 208000032839 leukemia Diseases 0.000 claims 1
- 210000001616 monocyte Anatomy 0.000 claims 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062983511P | 2020-02-28 | 2020-02-28 | |
| US62/983,511 | 2020-02-28 | ||
| PCT/US2021/019917 WO2021174004A1 (en) | 2020-02-28 | 2021-02-26 | Method for producing natural killer cells from pluripotent stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023516632A JP2023516632A (ja) | 2023-04-20 |
| JPWO2021174004A5 true JPWO2021174004A5 (zh) | 2024-03-05 |
Family
ID=75108897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022552125A Pending JP2023516632A (ja) | 2020-02-28 | 2021-02-26 | 多能性幹細胞からナチュラルキラー細胞を産生するための方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20210292713A1 (zh) |
| EP (1) | EP4110904A1 (zh) |
| JP (1) | JP2023516632A (zh) |
| KR (1) | KR20220148174A (zh) |
| CN (1) | CN115427555A (zh) |
| AR (1) | AR121455A1 (zh) |
| AU (1) | AU2021225952A1 (zh) |
| BR (1) | BR112022017216A2 (zh) |
| CA (1) | CA3169617A1 (zh) |
| CO (1) | CO2022011682A2 (zh) |
| IL (1) | IL295964A (zh) |
| MX (1) | MX2022010521A (zh) |
| TW (1) | TW202146646A (zh) |
| WO (1) | WO2021174004A1 (zh) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022144632A1 (en) | 2020-12-30 | 2022-07-07 | Crispr Therapeutics Ag | Compositions and methods for differentiating stem cells into nk cells |
| CN113801846B (zh) * | 2021-09-22 | 2024-06-04 | 南京艾尔普再生医学科技有限公司 | 一种从人诱导多能干细胞分化为自然杀伤细胞的方法 |
| CN114350608B (zh) * | 2022-01-27 | 2024-05-28 | 昭泰英基生物医药（香港）有限公司 | 一种诱导t细胞重编程为类nk细胞的组合物及其应用 |
| CN114517176B (zh) * | 2022-01-27 | 2022-12-02 | 广东普罗凯融生物医药科技有限公司 | 一种将ips细胞诱导成nk细胞的试剂盒及其应用方法 |
| WO2024101862A1 (ko) * | 2022-11-07 | 2024-05-16 | 마루테라퓨틱스 주식회사 | Nk 세포의 분화 효율 및 기능성 증가를 위한 배양 방법 |
| KR20240076715A (ko) * | 2022-11-17 | 2024-05-30 | 주식회사 씨티셀즈 | 미분화 줄기세포로부터 자연 살해 세포로의 분화 방법 및 이의 용도 |
| CN115537397B (zh) * | 2022-12-02 | 2023-03-24 | 广东先康达细胞库有限公司 | Nk细胞诱导培养基及其培养方法 |
| CN116410336B (zh) * | 2023-06-02 | 2023-09-22 | 云南赛元生物技术有限公司 | 一种嵌合抗原受体的编码核苷酸、car-nk细胞及其构建方法和应用 |
| CN117050940B (zh) * | 2023-10-11 | 2024-01-26 | 苏州艾凯利元生物科技有限公司 | 一种制备自然杀伤细胞的方法 |
| CN117050941B (zh) * | 2023-10-11 | 2024-01-26 | 苏州艾凯利元生物科技有限公司 | 一种制备自然杀伤细胞的方法 |
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| US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5139941A (en) | 1985-10-31 | 1992-08-18 | University Of Florida Research Foundation, Inc. | AAV transduction vectors |
| US6013516A (en) | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| US5994136A (en) | 1997-12-12 | 1999-11-30 | Cell Genesys, Inc. | Method and means for producing high titer, safe, recombinant lentivirus vectors |
| US9453219B2 (en) | 2003-05-15 | 2016-09-27 | Mello Biotech Taiwan Co., Ltd. | Cosmetic designs and products using intronic RNA |
| EP2208786B1 (en) | 2005-12-13 | 2018-08-01 | Kyoto University | Nuclear reprogramming factor |
| CN101743306A (zh) | 2007-03-23 | 2010-06-16 | 威斯康星校友研究基金会 | 体细胞重编程 |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| EP3078738B1 (en) | 2007-08-31 | 2020-05-20 | Whitehead Institute for Biomedical Research | Wnt pathway stimulation in reprogramming somatic cells |
| KR101564044B1 (ko) | 2007-10-31 | 2015-10-28 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 핵초기화 방법 |
| WO2009075119A1 (ja) | 2007-12-10 | 2009-06-18 | Kyoto University | 効率的な核初期化方法 |
| CA2709566A1 (en) | 2007-12-17 | 2009-06-25 | Gliamed, Inc. | Stem-like cells and method for reprogramming adult mammalian somatic cells |
| EP2229444B1 (en) | 2008-01-16 | 2019-10-30 | Shi-Lung Lin | Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant rna agents |
| EP2250252A2 (en) | 2008-02-11 | 2010-11-17 | Cambridge Enterprise Limited | Improved reprogramming of mammalian cells, and the cells obtained |
| EP2090649A1 (en) | 2008-02-13 | 2009-08-19 | Fondazione Telethon | Method for reprogramming differentiated cells |
| US20110014164A1 (en) | 2008-02-15 | 2011-01-20 | President And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
| CA2718904C (en) | 2008-03-17 | 2017-01-03 | The Scripps Research Institute | Combined chemical and genetic approaches for generation of induced pluripotent stem cells |
| WO2009114949A1 (en) | 2008-03-20 | 2009-09-24 | UNIVERSITé LAVAL | Methods for deprogramming somatic cells and uses thereof |
| EP2274424A4 (en) | 2008-04-07 | 2012-02-01 | Nupotential Inc | REPROGRAMMING A CELL BY INDUCING A PLURIPOTENT GENE USING AN HDAC MODULATOR |
| JP5340265B2 (ja) | 2008-06-27 | 2013-11-13 | 国立大学法人京都大学 | 効率的な人工多能性幹細胞の樹立方法 |
| WO2010009015A2 (en) | 2008-07-14 | 2010-01-21 | Oklahoma Medical Research Foundation | Production of pluripotent cells through inhibition of bright/arid3a function |
| WO2010147612A1 (en) | 2009-06-18 | 2010-12-23 | Lixte Biotechnology, Inc. | Methods of modulating cell regulation by inhibiting p53 |
| WO2010033920A2 (en) | 2008-09-19 | 2010-03-25 | Whitehead Institute For Biomedical Research | Compositions and methods for enhancing cell reprogramming |
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| WO2010042800A1 (en) | 2008-10-10 | 2010-04-15 | Nevada Cancer Institute | Methods of reprogramming somatic cells and methods of use for such cells |
| WO2010050626A1 (en) | 2008-10-30 | 2010-05-06 | Kyoto University | Method for producing induced pluripotent stem cells |
| WO2010056831A2 (en) | 2008-11-12 | 2010-05-20 | Nupotential, Inc. | Reprogramming a cell by inducing a pluripotent gene through use of an hdac modulator |
| US9045737B2 (en) | 2008-12-13 | 2015-06-02 | Dnamicroarray, Inc. | Artificial three-dimensional microenvironment niche culture |
| EP2401372A4 (en) | 2009-02-27 | 2012-09-19 | Univ Kyoto | NEW NUCLEAR REPROGRAMMING SUBSTANCE |
| WO2010102267A2 (en) | 2009-03-06 | 2010-09-10 | Ipierian, Inc. | Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells |
| WO2010111409A2 (en) | 2009-03-25 | 2010-09-30 | The Salk Institute For Biological Studies | Pluripotent stem cells |
| US20120076762A1 (en) | 2009-03-25 | 2012-03-29 | The Salk Institute For Biological Studies | Induced pluripotent stem cell generation using two factors and p53 inactivation |
| WO2010115050A2 (en) | 2009-04-01 | 2010-10-07 | The Regents Of The University Of California | Embryonic stem cell specific micrornas promote induced pluripotency |
| US20110088107A1 (en) | 2009-04-24 | 2011-04-14 | Yaqub Hanna | Compositions and methods for deriving or culturing pluripotent cells |
| WO2010147395A2 (en) | 2009-06-16 | 2010-12-23 | Korea Research Institute Of Bioscience And Biotechnology | Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same |
| TWI654206B (zh) | 2013-03-16 | 2019-03-21 | 諾華公司 | 使用人類化抗－ｃｄ１９嵌合抗原受體治療癌症 |
| EP3365433A1 (en) * | 2015-10-20 | 2018-08-29 | FUJIFILM Cellular Dynamics, Inc. | Methods for directed differentiation of pluripotent stem cells to immune cells |
| CA3003152A1 (en) * | 2015-11-04 | 2017-05-11 | Fate Therapeutics, Inc. | Methods and compositions for inducing hematopoietic cell differentiation |
-
2021
- 2021-02-26 CN CN202180017317.4A patent/CN115427555A/zh active Pending
- 2021-02-26 AR ARP210100517A patent/AR121455A1/es unknown
- 2021-02-26 IL IL295964A patent/IL295964A/en unknown
- 2021-02-26 WO PCT/US2021/019917 patent/WO2021174004A1/en unknown
- 2021-02-26 TW TW110107162A patent/TW202146646A/zh unknown
- 2021-02-26 JP JP2022552125A patent/JP2023516632A/ja active Pending
- 2021-02-26 KR KR1020227028995A patent/KR20220148174A/ko unknown
- 2021-02-26 MX MX2022010521A patent/MX2022010521A/es unknown
- 2021-02-26 AU AU2021225952A patent/AU2021225952A1/en active Pending
- 2021-02-26 BR BR112022017216A patent/BR112022017216A2/pt unknown
- 2021-02-26 US US17/186,877 patent/US20210292713A1/en active Pending
- 2021-02-26 EP EP21713302.4A patent/EP4110904A1/en active Pending
- 2021-02-26 CA CA3169617A patent/CA3169617A1/en active Pending
-
2022
- 2022-08-19 CO CONC2022/0011682A patent/CO2022011682A2/es unknown
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