JPWO2021133829A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2021133829A5 JPWO2021133829A5 JP2022538726A JP2022538726A JPWO2021133829A5 JP WO2021133829 A5 JPWO2021133829 A5 JP WO2021133829A5 JP 2022538726 A JP2022538726 A JP 2022538726A JP 2022538726 A JP2022538726 A JP 2022538726A JP WO2021133829 A5 JPWO2021133829 A5 JP WO2021133829A5
- Authority
- JP
- Japan
- Prior art keywords
- cas12l
- polypeptide
- guide rna
- nucleic acid
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920001184 polypeptide Polymers 0.000 claims 50
- 108090000765 processed proteins & peptides Proteins 0.000 claims 50
- 102000004196 processed proteins & peptides Human genes 0.000 claims 50
- 108020005004 Guide RNA Proteins 0.000 claims 22
- 150000007523 nucleic acids Chemical class 0.000 claims 20
- 108020004707 nucleic acids Proteins 0.000 claims 19
- 102000039446 nucleic acids Human genes 0.000 claims 19
- 230000004927 fusion Effects 0.000 claims 18
- 239000002773 nucleotide Substances 0.000 claims 16
- 125000003729 nucleotide group Chemical group 0.000 claims 16
- 108020004414 DNA Proteins 0.000 claims 15
- 230000003321 amplification Effects 0.000 claims 12
- 238000000034 method Methods 0.000 claims 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims 12
- 230000000694 effects Effects 0.000 claims 10
- 125000003275 alpha amino acid group Chemical group 0.000 claims 5
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 4
- 239000013604 expression vector Substances 0.000 claims 4
- 108020004999 messenger RNA Proteins 0.000 claims 4
- 238000003259 recombinant expression Methods 0.000 claims 4
- 108060004795 Methyltransferase Proteins 0.000 claims 3
- 238000006073 displacement reaction Methods 0.000 claims 3
- 230000035897 transcription Effects 0.000 claims 3
- 238000013518 transcription Methods 0.000 claims 3
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 2
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102100034343 Integrase Human genes 0.000 claims 2
- 238000007397 LAMP assay Methods 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 102000018120 Recombinases Human genes 0.000 claims 2
- 108010091086 Recombinases Proteins 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 108020000946 Bacterial DNA Proteins 0.000 claims 1
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 claims 1
- 108010061833 Integrases Proteins 0.000 claims 1
- 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 claims 1
- 102000016397 Methyltransferase Human genes 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 102000008579 Transposases Human genes 0.000 claims 1
- 108010020764 Transposases Proteins 0.000 claims 1
- 108020005202 Viral DNA Proteins 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 125000004122 cyclic group Chemical group 0.000 claims 1
- 230000009615 deamination Effects 0.000 claims 1
- 238000006481 deamination reaction Methods 0.000 claims 1
- 230000007423 decrease Effects 0.000 claims 1
- 230000027832 depurination Effects 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 238000011901 isothermal amplification Methods 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 239000002502 liposome Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 230000010076 replication Effects 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962952909P | 2019-12-23 | 2019-12-23 | |
| US62/952,909 | 2019-12-23 | ||
| PCT/US2020/066672 WO2021133829A1 (en) | 2019-12-23 | 2020-12-22 | Crispr-cas effector polypeptides and methods of use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023508362A JP2023508362A (ja) | 2023-03-02 |
| JPWO2021133829A5 true JPWO2021133829A5 (pt) | 2023-12-01 |
Family
ID=76573115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022538726A Pending JP2023508362A (ja) | 2019-12-23 | 2020-12-22 | Ｃｒｉｓｐｒ－ｃａｓエフェクターポリペプチド及びその使用方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20230028178A1 (pt) |
| EP (1) | EP4081533A4 (pt) |
| JP (1) | JP2023508362A (pt) |
| KR (1) | KR20220130686A (pt) |
| CN (1) | CN115698041A (pt) |
| AU (1) | AU2020412714A1 (pt) |
| BR (1) | BR112022009670A2 (pt) |
| CA (1) | CA3158403A1 (pt) |
| IL (1) | IL292896A (pt) |
| MX (1) | MX2022007858A (pt) |
| WO (1) | WO2021133829A1 (pt) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112023024985A2 (pt) | 2021-06-01 | 2024-02-20 | Arbor Biotechnologies Inc | Sistemas de edição de genes compreendendo uma crispr nuclease e usos dos mesmos |
| EP4373963A2 (en) | 2021-07-21 | 2024-05-29 | Montana State University | Nucleic acid detection using type iii crispr complex |
| WO2023039346A1 (en) * | 2021-09-10 | 2023-03-16 | The Regents Of The University Of California | Temperature regulated crispr-cas systems and methods of use thereof |
| WO2023240157A2 (en) | 2022-06-08 | 2023-12-14 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of dmd |
| WO2023250384A2 (en) * | 2022-06-22 | 2023-12-28 | The Regents Of The University Of California | Crispr-cas effector polypeptides and methods of use thereof |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2072527A1 (en) * | 2007-12-21 | 2009-06-24 | Altonabiotec AG | Fusion polypeptides comprising a SHBG dimerization component and uses thereof |
| RU2721275C2 (ru) * | 2012-12-12 | 2020-05-18 | Те Брод Инститьют, Инк. | Доставка, конструирование и оптимизация систем, способов и композиций для манипуляции с последовательностями и применения в терапии |
| DK3597740T3 (da) * | 2014-11-06 | 2022-06-20 | Dupont Us Holding Llc | Peptidmedieret indføring af rna-styret endonuklease i celler |
| KR20230170126A (ko) * | 2016-09-30 | 2023-12-18 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | Rna-가이드된 핵산 변형 효소 및 이의 사용 방법 |
| GB2569734B (en) * | 2016-09-30 | 2022-09-07 | Univ California | RNA-guided nucleic acid modifying enzymes and methods of use thereof |
| US20200255858A1 (en) * | 2017-11-01 | 2020-08-13 | Jillian F. Banfield | Casy compositions and methods of use |
| US10253365B1 (en) * | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
| US20220282308A1 (en) * | 2019-09-09 | 2022-09-08 | Arbor Biotechnologies, Inc. | Novel crispr dna targeting enzymes and systems |
-
2020
- 2020-12-22 JP JP2022538726A patent/JP2023508362A/ja active Pending
- 2020-12-22 AU AU2020412714A patent/AU2020412714A1/en active Pending
- 2020-12-22 BR BR112022009670A patent/BR112022009670A2/pt not_active Application Discontinuation
- 2020-12-22 CA CA3158403A patent/CA3158403A1/en active Pending
- 2020-12-22 MX MX2022007858A patent/MX2022007858A/es unknown
- 2020-12-22 WO PCT/US2020/066672 patent/WO2021133829A1/en unknown
- 2020-12-22 KR KR1020227023340A patent/KR20220130686A/ko unknown
- 2020-12-22 IL IL292896A patent/IL292896A/en unknown
- 2020-12-22 US US17/781,674 patent/US20230028178A1/en active Pending
- 2020-12-22 CN CN202080096862.2A patent/CN115698041A/zh active Pending
- 2020-12-22 EP EP20907560.5A patent/EP4081533A4/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Polidoros et al. | Rolling circle amplification-RACE: a method for simultaneous isolation of 5′ and 3′ cDNA ends from amplified cDNA templates | |
| Zhu et al. | Syn5 RNA polymerase synthesizes precise run-off RNA products | |
| US9255291B2 (en) | Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing | |
| JP7292258B2 (ja) | 縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変 | |
| US11761037B1 (en) | Probe and method of enriching target region applicable to high-throughput sequencing using the same | |
| EA038500B1 (ru) | Термостабильные нуклеазы cas9 | |
| JP2022111117A5 (pt) | ||
| CN102301010A (zh) | 闭合线状dna的制备 | |
| US11401543B2 (en) | Methods and compositions for improving removal of ribosomal RNA from biological samples | |
| JP5200302B2 (ja) | ヘリカーゼを使用するｒｎａターゲットの同定 | |
| JP6583796B2 (ja) | 核酸の増幅方法 | |
| Liu et al. | Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes | |
| WO2023246032A1 (zh) | 一锅法单链DNA环化扩增和CRISPR/Cas介导的核酸分子检测方法 | |
| US10858694B2 (en) | Methods and reagents for reverse-transcription polymerase chain reaction | |
| US20240200047A1 (en) | Enzymes with ruvc domains | |
| JPWO2021133829A5 (pt) | ||
| CN114391043B (zh) | 哺乳动物dna的甲基化检测及分析 | |
| WO2023093060A1 (zh) | 基于重组酶聚合酶扩增技术的逆转录扩增系统和方法 | |
| CN104059905B (zh) | 室温扩增dna的方法 | |
| Fujii et al. | Error-prone rolling circle amplification greatly simplifies random mutagenesis | |
| Takahashi et al. | Cell-free cloning using multiply-primed rolling circle amplification with modified RNA primers | |
| JP2017534297A (ja) | トランスポゼースによる核酸の切断及びリンカー付加の方法及び試薬 | |
| Kirsch et al. | A streamlined protocol for the detection of mRNA–sRNA interactions using AMT-crosslinking in vitro | |
| CN111850100A (zh) | 核酸扩增方法及其应用 | |
| JPWO2021050755A5 (pt) |