JPWO2019241438A5 - - Google Patents
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- JPWO2019241438A5 JPWO2019241438A5 JP2020569121A JP2020569121A JPWO2019241438A5 JP WO2019241438 A5 JPWO2019241438 A5 JP WO2019241438A5 JP 2020569121 A JP2020569121 A JP 2020569121A JP 2020569121 A JP2020569121 A JP 2020569121A JP WO2019241438 A5 JPWO2019241438 A5 JP WO2019241438A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- protein
- peptide
- polypeptide
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 229920001184 polypeptide Polymers 0.000 claims description 263
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 114
- YHIPILPTUVMWQT-UHFFFAOYSA-N Coelenterazine Chemical group C1=CC(O)=CC=C1CC(C(N1C=C(N2)C=3C=CC(O)=CC=3)=O)=NC1=C2CC1=CC=CC=C1 YHIPILPTUVMWQT-UHFFFAOYSA-N 0.000 claims description 57
- 230000004927 fusion Effects 0.000 claims description 54
- 239000000758 substrate Substances 0.000 claims description 40
- 230000003993 interaction Effects 0.000 claims description 39
- 150000007523 nucleic acids Chemical group 0.000 claims description 22
- 238000001514 detection method Methods 0.000 claims description 12
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 238000005755 formation reaction Methods 0.000 claims description 4
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 6
- 230000001809 detectable Effects 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 description 158
- 108090000623 proteins and genes Proteins 0.000 description 158
- 230000000295 complement Effects 0.000 description 65
- 108090000765 processed proteins & peptides Proteins 0.000 description 64
- 102000004196 processed proteins & peptides Human genes 0.000 description 64
- 108010016626 Dipeptides Proteins 0.000 description 62
- 102000004965 antibodies Human genes 0.000 description 39
- 108090001123 antibodies Proteins 0.000 description 39
- 239000000523 sample Substances 0.000 description 37
- 238000004458 analytical method Methods 0.000 description 33
- 101700058282 M Proteins 0.000 description 26
- 108060006943 RdRp Proteins 0.000 description 26
- 101710033820 XVIII Proteins 0.000 description 26
- 101700018328 ccdB Proteins 0.000 description 26
- 239000000203 mixture Substances 0.000 description 26
- 101700045377 mvp1 Proteins 0.000 description 26
- 150000001413 amino acids Chemical class 0.000 description 24
- 239000000427 antigen Substances 0.000 description 23
- 102000038129 antigens Human genes 0.000 description 23
- 108091007172 antigens Proteins 0.000 description 23
- 239000003596 drug target Substances 0.000 description 22
- 239000003814 drug Substances 0.000 description 16
- 229940079593 drugs Drugs 0.000 description 16
- 108020004707 nucleic acids Proteins 0.000 description 16
- 229920002395 Aptamer Polymers 0.000 description 13
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 13
- 229920002224 Peptide nucleic acid Polymers 0.000 description 13
- 238000005415 bioluminescence Methods 0.000 description 13
- 230000029918 bioluminescence Effects 0.000 description 13
- 238000004020 luminiscence type Methods 0.000 description 13
- 239000002751 oligonucleotide probe Substances 0.000 description 13
- 230000003834 intracellular Effects 0.000 description 12
- 210000001519 tissues Anatomy 0.000 description 10
- 150000003384 small molecules Chemical class 0.000 description 9
- 108010043958 Peptoids Proteins 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 239000012491 analyte Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 239000012139 lysis buffer Substances 0.000 description 3
- 210000004027 cells Anatomy 0.000 description 2
- 230000001413 cellular Effects 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 230000002934 lysing Effects 0.000 description 2
- 210000000056 organs Anatomy 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N HEPES Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- PNNNRSAQSRJVSB-BXKVDMCESA-N Rhamnose Chemical compound C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O PNNNRSAQSRJVSB-BXKVDMCESA-N 0.000 description 1
- 239000012131 assay buffer Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002860 competitive Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N oxo-oxoalumanyloxy-[oxo(oxoalumanyloxy)silyl]oxysilane;dihydrate Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024005285A JP2024054136A (ja) | 2018-06-12 | 2024-01-17 | 多分子ルシフェラーゼ |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862684014P | 2018-06-12 | 2018-06-12 | |
| US62/684,014 | 2018-06-12 | ||
| PCT/US2019/036844 WO2019241438A2 (fr) | 2018-06-12 | 2019-06-12 | Luciférase multipartite |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024005285A Division JP2024054136A (ja) | 2018-06-12 | 2024-01-17 | 多分子ルシフェラーゼ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2021526832A JP2021526832A (ja) | 2021-10-11 |
| JPWO2019241438A5 true JPWO2019241438A5 (fr) | 2022-06-17 |
Family
ID=68842790
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020569121A Pending JP2021526832A (ja) | 2018-06-12 | 2019-06-12 | 多分子ルシフェラーゼ |
| JP2024005285A Pending JP2024054136A (ja) | 2018-06-12 | 2024-01-17 | 多分子ルシフェラーゼ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024005285A Pending JP2024054136A (ja) | 2018-06-12 | 2024-01-17 | 多分子ルシフェラーゼ |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20200270586A1 (fr) |
| EP (1) | EP3807419A4 (fr) |
| JP (2) | JP2021526832A (fr) |
| CN (1) | CN112567028A (fr) |
| WO (1) | WO2019241438A2 (fr) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022509200A (ja) | 2018-11-28 | 2022-01-20 | プロメガ コーポレイション | 反応性ペプチド標識付け |
| JP2022526907A (ja) | 2019-03-20 | 2022-05-27 | プロメガ コーポレイション | 光親和性プローブ |
| US11360096B2 (en) * | 2019-05-16 | 2022-06-14 | Duke University | Complex BRET technique for measuring biological interactions |
| CA3158729A1 (fr) * | 2019-11-27 | 2021-06-03 | Virginia Kincaid | Peptides et polypeptides de luciferase multipartites |
| WO2021231481A1 (fr) * | 2020-05-11 | 2021-11-18 | Chan Zuckerberg Biohub, Inc. | Dosage de détection du virus sars-cov-2 |
| EP4157854A2 (fr) * | 2020-05-27 | 2023-04-05 | University of Washington | Plate-forme de biocapteur modulaire et généralisable basée sur des commutateurs de protéine conçus de novo |
| CN113461809A (zh) * | 2020-07-17 | 2021-10-01 | 南京诺唯赞生物科技股份有限公司 | 筛选结合抑制剂和/或结合区域的方法 |
| US20220064696A1 (en) | 2020-08-28 | 2022-03-03 | Promega Corporation | Target engagement assay for ras proteins |
| US20220178923A1 (en) * | 2020-12-04 | 2022-06-09 | Brigham Young University | Method for detection of viral infections using split enzymes |
| CN112694535B (zh) * | 2021-01-05 | 2023-03-31 | 重庆医科大学 | 用于抗体检测的多功能蛋白分子开关 |
| US20240132859A1 (en) | 2022-05-04 | 2024-04-25 | Promega Corporation | Modified dehalogenase with extended surface loop regions |
| US20240174992A1 (en) | 2022-05-04 | 2024-05-30 | Promega Corporation | Split modified dehalogenase variants |
| WO2023215432A1 (fr) | 2022-05-04 | 2023-11-09 | Promega Corporation | Variants de déshalogénase à permutation circulaire |
| CN116068198B (zh) * | 2022-11-30 | 2024-01-09 | 深圳湾实验室 | Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用 |
| CN117330755B (zh) * | 2023-08-25 | 2024-03-26 | 广东省农业科学院农业生物基因研究中心 | 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110136730A1 (en) * | 2008-04-18 | 2011-06-09 | John Fuller Atkins | "Method for screening compounds comprising the use of picornavirus protease 2A" |
| WO2013177255A2 (fr) * | 2012-05-24 | 2013-11-28 | Connecticut College | Luciférases chimériques |
| SG10201601929YA (en) * | 2013-03-15 | 2016-04-28 | Promega Corp | Activation Of Bioluminescence By Structural Complementation |
| US10634680B2 (en) * | 2016-04-26 | 2020-04-28 | University Of Utah Research Foundation | Target-binding activated split reporter systems for analyte detection and related components and methods |
-
2019
- 2019-06-12 JP JP2020569121A patent/JP2021526832A/ja active Pending
- 2019-06-12 CN CN201980053139.3A patent/CN112567028A/zh active Pending
- 2019-06-12 EP EP19820498.4A patent/EP3807419A4/fr active Pending
- 2019-06-12 US US16/439,565 patent/US20200270586A1/en active Pending
- 2019-06-12 WO PCT/US2019/036844 patent/WO2019241438A2/fr unknown
-
2024
- 2024-01-17 JP JP2024005285A patent/JP2024054136A/ja active Pending
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