JPWO2008081539A1 - Supplements containing useful bacteria such as lactic acid bacteria using porous silica - Google Patents
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 title claims abstract description 82
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 66
- 241000894006 Bacteria Species 0.000 title claims abstract description 40
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 33
- 239000004310 lactic acid Substances 0.000 title claims abstract description 33
- 239000000377 silicon dioxide Substances 0.000 title claims abstract description 21
- 239000013589 supplement Substances 0.000 title claims abstract description 13
- 239000005909 Kieselgur Substances 0.000 claims abstract description 32
- 241000233866 Fungi Species 0.000 claims abstract description 20
- 210000000936 intestine Anatomy 0.000 claims abstract description 17
- 239000002253 acid Substances 0.000 claims description 10
- 239000000741 silica gel Substances 0.000 claims description 7
- 229910002027 silica gel Inorganic materials 0.000 claims description 7
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 14
- 230000006835 compression Effects 0.000 abstract description 4
- 238000007906 compression Methods 0.000 abstract description 4
- 235000013618 yogurt Nutrition 0.000 description 14
- 235000013336 milk Nutrition 0.000 description 9
- 239000008267 milk Substances 0.000 description 9
- 210000004080 milk Anatomy 0.000 description 9
- 210000004051 gastric juice Anatomy 0.000 description 8
- 239000003610 charcoal Substances 0.000 description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000011707 mineral Substances 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 210000002784 stomach Anatomy 0.000 description 5
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000000748 compression moulding Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 238000000635 electron micrograph Methods 0.000 description 2
- 235000021109 kimchi Nutrition 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 235000013557 nattō Nutrition 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- -1 stomach acid Chemical class 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
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Abstract
【課題】本発明は、乳酸菌等有用菌類を、生きたまま、安定に、腸内に送り届ける多孔質シリカを用いた乳酸菌等有用菌含有サプリメントを提供する。【解決手段】本発明の乳酸菌等有用菌類の担持材として、珪藻土及び多孔質シリカが菌類に無害であり、多孔質空間内を利用できることから、休眠胞子の物理的機械的担持（多孔質シリカと休眠胞子を混合し、そのまま圧縮成型）、培地ごと乾燥休眠させ担持する（多孔質シリカと混合した培地を乾燥させ、同時に菌類を休眠させ、それらを圧縮成型）等が選択できる。【選択図】なしThe present invention provides a supplement containing useful bacteria such as lactic acid bacteria using porous silica that delivers useful fungi such as lactic acid bacteria to the intestine stably and alive. As a supporting material for useful fungi such as lactic acid bacteria of the present invention, diatomaceous earth and porous silica are harmless to fungi and can be used in a porous space. The dormant spores are mixed and compression molded as it is, and the medium is dried and rested and supported (the medium mixed with the porous silica is dried, and at the same time the fungi are dormant, and they are compression molded). [Selection figure] None
Description
本発明は、乳酸菌等、人体及び生物に有用な菌類を生きたまま安定に腸内へ送りと届ける多孔質シリカを用いた乳酸菌等有用菌含有サプリメントに関する。 The present invention relates to a supplement containing useful bacteria such as lactic acid bacteria using porous silica that stably delivers living fungi useful for human bodies and living organisms such as lactic acid bacteria to the intestines.
従来、乳酸菌等有用菌類の腸への生きたままの供給は、胃内部での強酸性に耐性を持つ菌類の開発や、ゼラチン等のカプセルを用いて行われている。
このような供給方法は、前者の場合、ヨーグルト等の継続摂取を必要とし、後者の場合は、摂取者の体質により、うまく腸内で溶けなかったり、逆に胃酸で消化されてしまう等の問題がある。
このような問題を解決するため、炭を用いて微生物を腸へ送る方法の技術が知られている。
この公知技術は、粉末状の炭を乾燥させ、減菌処理を施し、ヨーグルト、乳酸菌飲料、納豆、キムチに炭を混ぜ、時間の経過により炭の表面に存在するミクロンサイズの穴まで素材が浸透するまで待ち、ヨーグルト、乳酸菌飲料、納豆、キムチに炭を混ぜたものを飲み込むことで、粉末状の炭が微生物に胃液で変質しない隠れ家的スペースを与え、胃の中において胃液に触れず、口から飲んだときと同じ状態の微生物を腸まで容易に送ることができるものである（特許文献１を参照）。
しかしながら、これまでの報告で明らかになったように、耐酸性菌も完全ではなく、ヨーグルトの継続摂取も、飽きがくることから難しい。
また，カプセルの利用も摂取者の体質に合わせた調整が難しく、万人に対して、安定に有用菌を生きたまま腸内に送り届けることはできない。
Such a supply method requires continuous ingestion of yogurt or the like in the former case, and in the latter case, it does not dissolve well in the intestine due to the constitution of the ingestor or conversely digests with stomach acid. There is.
In order to solve such a problem, a technique of sending a microorganism to the intestine using charcoal is known.
In this known technology, powdered charcoal is dried, sterilized, mixed with yogurt, lactic acid bacteria beverages, natto and kimchi, and the material penetrates into micron-sized holes on the charcoal surface over time. Wait until it is swallowed, yogurt, lactic acid bacteria beverage, natto, kimchi mixed with charcoal, powdered charcoal gives microorganisms a hideaway space that does not change with gastric juice, does not touch gastric juice in the stomach, mouth The microorganisms in the same state as when drinking from can be easily sent to the intestines (see Patent Document 1).
However, as revealed by previous reports, acid-resistant bacteria are not perfect, and continuous intake of yogurt is difficult because it makes you tired.
In addition, the use of capsules is also difficult to adjust according to the constitution of the ingestor, and it is impossible to deliver useful bacteria stably to the intestines to all people.
本発明は、乳酸菌等有用菌類を、生きたまま、安定に、腸内に送り届ける多孔質シリカを用いた乳酸菌等有用菌含有サプリメントを提供することを目的とする。 An object of the present invention is to provide a supplement containing useful bacteria such as lactic acid bacteria using porous silica that delivers useful fungi such as lactic acid bacteria to the intestine stably and alive.
本発明の乳酸菌等有用菌類担持材は、天然鉱物資源である珪藻土及びシリカゲル等の人工多孔質シリカを圧縮成型したものである。
本発明のサプリメントを胃液等の酸から守るコーティング材は、人体や生物に無害で、腸内で分解される炭水化物等である。The useful fungi-supporting material such as lactic acid bacteria of the present invention is obtained by compression-molding natural porous minerals such as diatomaceous earth and silica gel.
The coating material that protects the supplement of the present invention from acid such as gastric juice is a carbohydrate that is harmless to the human body and living organisms and is decomposed in the intestine.
本発明の乳酸菌等有用菌類の担持材である、天然鉱物資源の珪藻土及びシリカゲル等の人工多孔質シリカは、胃酸等の強酸に影響を受けないこと、胃液の様な粘性の有る液体を吸水しないこと、菌類及び人体並びに生物に無害であることが確認されており、このような安全な素材は他に無く、加工・成型も容易であることから実用化の可能性も高い。
本発明のサプリメントに使用される天然鉱物資源の珪藻土及びシリカゲル等の人工多孔質シリカは、胃液の内部への浸透を防ぎ、耐酸性も高いことから、確実に腸に達することができる。さらに、胃を通過した後の十二指腸でアルカリ性膵液が分泌されるので、アルカリ性には弱いため、腸内で分解し、そこで内部に担持した有用菌類を放出する。The artificial porous silica such as diatomaceous earth and silica gel, which are natural mineral resources, which is a support material for useful fungi such as lactic acid bacteria of the present invention, is not affected by strong acids such as stomach acid, and does not absorb viscous liquids such as gastric juice In addition, it has been confirmed that it is harmless to fungi, human bodies and living organisms, and there is no other such safe material, and since it is easy to process and mold, there is a high possibility of practical application.
Artificial porous silica such as diatomaceous earth and silica gel, which are natural mineral resources used in the supplement of the present invention, prevents gastric juice from penetrating into the inside and has high acid resistance, so that it can reliably reach the intestines. Furthermore, since alkaline pancreatic juice is secreted in the duodenum after passing through the stomach, it is weak against alkalinity, so it decomposes in the intestine and releases useful fungi carried therein.
本発明の多孔質シリカを用いた乳酸菌等有用菌含有サプリメントの一実施例を以下に説明する。
本発明の乳酸菌等有用菌類の担持材として、胃液の酸性に耐えられ、人体及び生物に無害で、菌類を生きたまま供給でき、腸内のアルカリ性で分解する材料を検討した結果、天然鉱物資源の珪藻土及びシリカゲル等の人工多孔質シリカを選択した。
珪藻土は、植物性プランクトン（藻）が化石化したもので、それら化石が組み合わさって空間ができ、主成分はガラスと同じ珪酸質（ＳｉＯ２）で、平均粒径０．０５ｍｍの土に無数の孔が空いている超多孔質である。また、シリカゲル等の人工多孔質シリカも、珪藻土とほぼ同等の性質を持つ。
この珪藻土が持つ耐酸性、乳酸菌等有用菌類に対する無毒性、人体及び生物に対する安全性、腸内のアルカリ性環境下における分解性等を利用して、乳酸菌等有用菌類を安定に腸まで送り届ける錠剤（サプリメント）を作製する。
錠剤（サプリメント）は、珪藻土及び多孔質シリカに乳酸菌等有用菌類を担持させた後、珪藻土及び多孔質シリカを軽度に圧縮成型するだけで良いので製品化が容易である。
一方、錠剤（サプリメント）の表面のコーティング材は、人体及び生物に無害で、胃液の酸性に耐えられると同時に、腸内で分解される必要があるため、この様な性質を持つ、例えば澱粉などの炭水化物を使用する。
乳酸菌等有用菌類の担持は、下記の実験の結果から、珪藻土及び多孔質シリカが菌類に無害であり、多孔質空間内を利用できることから、休眠胞子の物理的機械的担持（多孔質シリカと休眠胞子を混合し、そのまま圧縮成型）、培地ごと乾燥休眠させ担持する（多孔質シリカと混合した培地を乾燥させ、同時に菌類を休眠させ、それらを圧縮成型）等が選択できる。One example of supplement containing useful bacteria such as lactic acid bacteria using the porous silica of the present invention will be described below.
As a support material for useful fungi such as lactic acid bacteria of the present invention, as a result of examining materials that can withstand the acidity of gastric juice, are harmless to the human body and living organisms, can be supplied alive, and decomposes alkaline in the intestines, natural mineral resources Artificial porous silica such as diatomaceous earth and silica gel was selected.
Diatomaceous earth is fossilized from phytoplankton (algae), and these fossils combine to create a space. The main component is the same siliceous (SiO 2 ) as glass, and countless in soil with an average particle size of 0.05 mm. It is super porous with pores. Artificial porous silica such as silica gel has almost the same properties as diatomaceous earth.
Tablets (supplements) that deliver useful fungi such as lactic acid bacteria stably to the intestines by utilizing the acid resistance, non-toxicity to useful fungi such as lactic acid bacteria, safety to humans and organisms, and degradability in an alkaline environment in the intestine. ).
Tablets (supplements) can be easily commercialized because diatomaceous earth and porous silica need only be lightly compression-molded after supporting useful fungi such as lactic acid bacteria on diatomaceous earth and porous silica.
On the other hand, the coating material on the surface of the tablet (supplement) is harmless to the human body and living organisms, can withstand the acidity of the gastric juice, and at the same time needs to be decomposed in the intestines. Use carbohydrates.
Based on the results of the following experiment, lactic acid bacteria and porous silica are supported by diatomaceous earth and porous silica, which are harmless to fungi and can be used in the porous space. Spores can be mixed and compression molded as it is, and the medium can be dried and rested and supported (the medium mixed with porous silica is dried, and at the same time the fungi are allowed to sleep, and they are compression molded).
次に、珪藻土等の多孔質シリカが乳酸菌に対して無毒であることを確認するため、珪藻土存在下での乳酸菌培養試験を行った。 Next, in order to confirm that porous silica such as diatomaceous earth is non-toxic to lactic acid bacteria, a lactic acid bacteria culture test in the presence of diatomaceous earth was conducted.
実験例１：珪藻土原土での乳酸菌培養
珪藻土の乳酸菌への無毒性を確認するため、珪藻土原土を粉砕し、等体積の牛乳と混練し、それに小さじ一杯のヨーグルトを添加し、３０℃、静置で乳酸菌の培養を行った。
その結果、２４時間後に牛乳部分は固化した。
生成物がヨーグルトかどうかを確認するため、７２時間後の同試料から、小さじ一杯の固化物を取り出し、７５mlの牛乳に添加した。
添加後、３０℃、静置、２４時間で、ヨーグルトＮＯ，１の生成を確認した。
２週間後、再び、最初の珪藻土と牛乳の混錬物から小さじ一杯の固形物を２００mlの牛乳に添加した。
添加後、３０℃、静置、２４時間で、やはりヨーグルトＮＯ，２の生成を確認した。
念のため、ヨーグルトＮＯ，１から小さじ一杯の固形物を２００mlの牛乳に添加して、ヨーグルト生成の確認を行った。
やはり、添加後、３０℃、静置、２４時間で、ヨーグルトの生成を確認した。Experimental Example 1: Lactic acid bacteria culture in diatomaceous earth To confirm the non-toxicity of diatomaceous earth to lactic acid bacteria, diatomaceous earth is pulverized and kneaded with an equal volume of milk, and a teaspoon of yogurt is added thereto, The lactic acid bacteria were cultured by standing.
As a result, the milk part solidified after 24 hours.
To ascertain whether the product is yogurt, a teaspoon of solidified material was removed from the same sample after 72 hours and added to 75 ml of milk.
After the addition, the production of yogurt NO, 1 was confirmed at 30 ° C. and standing for 24 hours.
Two weeks later again, a teaspoon of solids from the first diatomaceous earth and milk kneaded material was added to 200 ml of milk.
After the addition, the formation of yogurt NO, 2 was also confirmed at 30 ° C. and standing for 24 hours.
As a precaution, yogurt NO, 1 teaspoon of solids was added to 200 ml of milk to confirm the production of yogurt.
Again, after the addition, the production of yogurt was confirmed at 30 ° C. and standing for 24 hours.
実験例２：高温加熱処理をした珪藻土での乳酸菌培養
珪藻土原土は、硫黄等各種揮発性成分を含んでいるため、それらが高温で除去された状態でも乳酸菌類が安定に培養できるかどうかの確認を行う。
７００℃、２４時間焼成した珪藻土と等体積の牛乳を混錬し、実験例１と同様に、ヨーグルトを添加した。
その結果、３０℃、静置、２４時間で牛乳部分は固化した。
生成物がヨーグルトかどうかを確認するため、７２時間後の同試料から、小さじ一杯の固化物を取り出し、２００mlの牛乳に添加した。
添加後、３０℃、静置、２４時間で、ヨーグルトの生成を確認した。
以上の実験の結果、珪藻土原土及び焼成した珪藻土の両方において安定に乳酸菌が培養できることが確認された。
このことから、珪藻土が乳酸菌に無毒であり、多孔空間を利用することにより、乳酸菌等有用菌類を物理的機械的、或いは、培地ごと珪藻土及び多孔質シリカに担持させられることが明らかになった。Experimental example 2: Lactic acid bacteria culture in diatomaceous earth subjected to high-temperature heat treatment Since diatomaceous earth contains various volatile components such as sulfur, whether lactic acid bacteria can be stably cultured even when they are removed at high temperature Confirm.
A diatomaceous earth baked at 700 ° C. for 24 hours and an equal volume of milk were kneaded, and yogurt was added as in Experimental Example 1.
As a result, the milk part solidified at 30 ° C. and standing for 24 hours.
To ascertain whether the product is yogurt, a teaspoon of solidified material was removed from the same sample after 72 hours and added to 200 ml of milk.
After the addition, the production of yogurt was confirmed at 30 ° C. and standing for 24 hours.
As a result of the above experiment, it was confirmed that lactic acid bacteria can be stably cultured in both diatomaceous earth and calcined diatomaceous earth.
From this, it became clear that diatomaceous earth is non-toxic to lactic acid bacteria, and by utilizing the porous space, useful fungi such as lactic acid bacteria can be supported on diatomaceous earth and porous silica together with the physical or mechanical medium.
実験例３：珪藻土の圧縮成型（細孔組織の耐圧性の確認）
珪藻土原土は多孔質であるが、実際の錠剤等作製時には高い圧力がかかるため、これら細孔が加圧後も維持されているかどうかの確認を行う。
珪藻土原土を米粒大に粉砕し、３００ｔの圧力をプレス機で加え、成型を行った。
成型された試料を走査型電子顕微鏡で観察し、成型された試料の内部構造（図１を参照）と、珪藻土原土の内部構造（図２を参照）と比較を行った結果、加圧後も個々の珪藻化石は壊れておらず、内部の細孔構造が維持されていることが確認された。
以上の実験の結果、加圧成型を行っても、珪藻土内に細孔空間が維持され、乳酸菌を担持できることが確認された。Experimental example 3: compression molding of diatomaceous earth (confirmation of pressure resistance of pore structure)
Diatomaceous earth is porous, but high pressure is applied during the production of actual tablets and the like, so it is confirmed whether these pores are maintained after pressurization.
The diatomaceous earth was pulverized to a grain size, and 300 t of pressure was applied with a press to perform molding.
As a result of observing the molded sample with a scanning electron microscope and comparing the internal structure of the molded sample (see Fig. 1) with the internal structure of the diatomaceous earth (see Fig. 2), after pressurization It was confirmed that the individual diatom fossils were not broken and the internal pore structure was maintained.
As a result of the above experiments, it was confirmed that the pore space was maintained in the diatomaceous earth even when pressure molding was performed, and lactic acid bacteria could be supported.
本発明の乳酸菌等有用菌類担持材は、天然鉱物資源である珪藻土及びシリカゲル等の人工多孔質シリカを圧縮成型したものである（図３を参照）。
本発明のサプリメントを胃液等の酸から守るコーティング材は、人体や生物に無害で、腸内で分解される澱粉などの炭水化物である。The useful fungi-supporting material such as lactic acid bacteria of the present invention is obtained by compression-molding artificial porous silica such as diatomaceous earth and silica gel, which are natural mineral resources (see FIG. 3).
The coating material that protects the supplement of the present invention from acid such as gastric juice is a carbohydrate such as starch that is harmless to the human body and living organisms and decomposes in the intestine.
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| PCT/JP2006/326236 WO2008081539A1 (en) | 2006-12-28 | 2006-12-28 | Supplement containing useful bacterium such as lactic acid bacterium with the use of porous silica |
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| HU1000278D0 (en) * | 2010-05-28 | 2010-07-28 | Egis Gyogyszergyar Nyilvanosan | Novel pharmaceutical use uf silicic acid |
| BR112013027129A2 (en) * | 2011-04-22 | 2017-01-10 | Pepsico Inc | porous particle extract encapsulation |
| JP6468633B2 (en) * | 2014-10-03 | 2019-02-13 | 富士カプセル株式会社 | Three-layer structure seamless capsule |
| JP6640966B2 (en) * | 2018-12-05 | 2020-02-05 | 富士カプセル株式会社 | Three-layer seamless capsule |
| CN111012804B (en) * | 2019-12-16 | 2022-05-17 | 南京大学 | Probiotic mineral material composite preparation and application of mineral material in preparation of medicine for inhibiting malignant tumor growth |
| JP7222548B2 (en) * | 2019-12-26 | 2023-02-15 | 富士カプセル株式会社 | Three-layer structure seamless capsule |
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